RchiOBHm_Chr2g0129461

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000778
Physical Location & Seq
Reverse (-)
45111963 .. 45115504
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50110

Sequence Viewer

Length: 1458 bp
ATGGATTACAGCCTTTATATTTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTCTTACGTTCCTTTTTTCTACTTATAAGTCAAAATCCCATGAAAAATCCATTAATCTTCCCCCTGGCAGTTTTGGGTGGCCGATTATCGGCGAAACCTTTGCCTTTCTACACAATGACCATGAAAAATTTATAGGGGATAGAATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCGAACAGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTCCGTTCTTCATACCAAAAAGCTACTTCCATCATCATGCCAAAACAAATGGAAAAGCACGTATCTCAGGCACCGGGGTTTCTGAGAGCCGAAGCCCTTGTTGGCTATGTTGGAGCGATGGATTCCATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCACCTCACGCAGCTACTCGTTTTGACTCTCTCAGCCAAGTCATTCTTGGGACTCGAAGACCATTGTCGTATTGATAAGCTTGCGAAGTTGATGGACACCATGATGCCGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATCGAGCAATGAAAGCAGTGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGCGTCTACAGGAAACAACATGCATGATTTTTTGTCTTATATGATTGGTACGGCAGACCCGGTTACCGGAAGGTTCATGCCGGATAATGAAATTGCTGACAAAATCATGGGGATTGTGGCCGCTTCCTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATTTATGACAAAGTCCGAATTGAACAAATGGAGATTTTGAAGGCCAAAAAATTTGGCGAGGCACTCAATTGGGATGATCTGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAAAGGACTTCACTTACGAAGGTTATGCAATTCCTAAAGGATGGAAGGTTTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCAACAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTCGGGAGCGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

485

Amino Acids

54.94

Weight (kDa)

9.2

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 40 - 457 1.1e-54 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 84, 1346
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccBSI CCGCTC 1 cut(s) 1275
AccI GTMKAC 2 cut(s) 29, 761
AciI CCGC 3 cut(s) 876, 1247, 1275
AclWI GGATC 1 cut(s) 1211
AcoI YGGCCR 2 cut(s) 137, 873
AcsI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
AcuI CTGAAG 2 cut(s) 1067, 1442
AcyI GRCGYC 1 cut(s) 758
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
AgsI TTSAA 5 cut(s) 229, 886, 965, 982, 1354
AjnI CCWGG 3 cut(s) 121, 924, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 8 cut(s) 310, 346, 371, 544, 610, 896, 1439, 1454
AluI AGCT 8 cut(s) 310, 346, 371, 544, 610, 896, 1439, 1454
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 1211
AoxI GGCC 3 cut(s) 137, 873, 984
ApeKI GCWGC 4 cut(s) 317, 541, 707, 1103
ApoI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AseI ATTAAT 1 cut(s) 111
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 643, 1204
AspS9I GGNCC 1 cut(s) 1277
AsuC2I CCSGG 4 cut(s) 423, 669, 815, 1293
AsuHPI GGTGA 2 cut(s) 257, 524
AvaII GGWCC 1 cut(s) 1277
BanI GGYRCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 4 cut(s) 329, 553, 694, 1090
BccI CCATC 8 cut(s) 50, 386, 460, 500, 616, 1140, 1344, 1409
BceAI ACGGC 1 cut(s) 822
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 1112, 1146
BciT130I CCWGG 3 cut(s) 123, 926, 1055
BcnI CCSGG 4 cut(s) 423, 669, 815, 1293
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 2 cut(s) 644, 1205
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 5 cut(s) 318, 542, 708, 876, 1104
BlsI GCNGC 5 cut(s) 319, 543, 709, 877, 1105
Bme1390I CCNGG 7 cut(s) 123, 423, 669, 815, 926, 1055, 1293
Bme18I GGWCC 1 cut(s) 1277
BmgT120I GGNCC 1 cut(s) 1277
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 7 cut(s) 123, 423, 669, 815, 926, 1055, 1293
BmrI ACTGGG 2 cut(s) 511, 1440
BmsI GCATC 1 cut(s) 624
BmuI ACTGGG 2 cut(s) 511, 1440
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 594
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 2 cut(s) 876, 1076
BpuMI CCSGG 4 cut(s) 423, 669, 815, 1293
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 7 cut(s) 121, 422, 474, 668, 925, 1053, 1291
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
Bse118I RCCGGY 3 cut(s) 275, 637, 703
Bse1I ACTGG 4 cut(s) 506, 1163, 1409, 1446
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 3 cut(s) 123, 926, 1055
BseDI CCNNGG 7 cut(s) 121, 422, 474, 668, 925, 1053, 1291
BseGI GGATG 4 cut(s) 511, 1021, 1151, 1260
BseLI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 3 cut(s) 422, 428, 576
BseNI ACTGG 4 cut(s) 506, 1163, 1409, 1446
BseRI GAGGAG 2 cut(s) 205, 1233
BseXI GCAGC 4 cut(s) 329, 553, 694, 1090
BshFI GGCC 3 cut(s) 139, 875, 986
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 1172
BsiSI CCGG 9 cut(s) 276, 422, 638, 668, 704, 815, 822, 836, 1293
BslFI GGGAC 2 cut(s) 594, 685
BslI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
BsmAI GTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 594, 685
BsnI GGCC 3 cut(s) 139, 875, 986
Bso31I GGTCTC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 1172
Bsp143I GATC 3 cut(s) 1018, 1216, 1383
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 3 cut(s) 876, 1247, 1275
BspANI GGCC 3 cut(s) 139, 875, 986
BspCNI CTCAG 3 cut(s) 423, 427, 575
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 1 cut(s) 1211
BspQI GCTCTTC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 319
BsrBI CCGCTC 1 cut(s) 1275
BsrDI GCAATG 1 cut(s) 696
BsrFI RCCGGY 3 cut(s) 275, 637, 703
BsrI ACTGG 4 cut(s) 506, 1163, 1409, 1446
BssAI RCCGGY 3 cut(s) 275, 637, 703
BssECI CCNNGG 7 cut(s) 121, 422, 474, 668, 925, 1053, 1291
BssMI GATC 3 cut(s) 1018, 1216, 1383
BssNI GRCGYC 1 cut(s) 758
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 123, 926, 1055
Bst4CI ACNGT 2 cut(s) 256, 1165
Bst6I CTCTTC 3 cut(s) 353, 1086, 1203
BstACI GRCGYC 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 3 cut(s) 612, 639, 705
BstDEI CTNAG 4 cut(s) 414, 431, 562, 1322
BstDSI CCRYGG 1 cut(s) 474
BstEII GGTNACC 1 cut(s) 817
BstF5I GGATG 4 cut(s) 511, 1021, 1151, 1260
BstH2I RGCGCY 2 cut(s) 644, 1205
BstHHI GCGC 2 cut(s) 643, 1204
BstKTI GATC 3 cut(s) 1021, 1219, 1386
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 1018, 1216, 1383
BstMWI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
BstNI CCWGG 3 cut(s) 123, 926, 1055
BstNSI RCATGY 1 cut(s) 778
BstPAI GACNNNNGTC 1 cut(s) 594
BstPI GGTNACC 1 cut(s) 817
BstSCI CCNGG 7 cut(s) 121, 421, 667, 813, 924, 1053, 1291
BstSFI CTRYAG 1 cut(s) 762
BstV1I GCAGC 4 cut(s) 329, 553, 694, 1090
BstV2I GAAGAC 1 cut(s) 594
BsuRI GGCC 3 cut(s) 139, 875, 986
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 2 cut(s) 479, 1076
BtsCI GGATG 4 cut(s) 511, 1021, 1151, 1260
BtsI GCAGTG 2 cut(s) 270, 705
BtsIMutI CAGTG 5 cut(s) 261, 270, 499, 705, 1170
Cac8I GCNNGC 3 cut(s) 612, 639, 705
CfoI GCGC 2 cut(s) 643, 1204
Cfr10I RCCGGY 3 cut(s) 275, 637, 703
Cfr13I GGNCC 1 cut(s) 1277
CseI GACGC 1 cut(s) 747
Csp6I GTAC 1 cut(s) 804
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 4 cut(s) 414, 431, 562, 1322
DpnI GATC 3 cut(s) 1020, 1218, 1385
DpnII GATC 3 cut(s) 1018, 1216, 1383
EaeI YGGCCR 2 cut(s) 137, 873
Eam1104I CTCTTC 3 cut(s) 353, 1086, 1203
EarI CTCTTC 3 cut(s) 353, 1086, 1203
EciI GGCGGA 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 2 cut(s) 1067, 1442
Eco91I GGTNACC 1 cut(s) 817
EcoO65I GGTNACC 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 121, 924, 1053
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 474
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 594, 685
FblI GTMKAC 2 cut(s) 29, 761
Fnu4HI GCNGC 5 cut(s) 318, 542, 708, 876, 1104
FokI GGATG 4 cut(s) 518, 1028, 1158, 1247
Fsp4HI GCNGC 5 cut(s) 318, 542, 708, 876, 1104
FspBI CTAG 1 cut(s) 242
GlaI GCGC 2 cut(s) 642, 1203
GluI GCNGC 5 cut(s) 318, 542, 708, 876, 1104
GsuI CTGGAG 2 cut(s) 876, 1076
HaeII RGCGCY 2 cut(s) 644, 1205
HaeIII GGCC 3 cut(s) 139, 875, 986
HapII CCGG 9 cut(s) 276, 422, 638, 668, 704, 815, 822, 836, 1293
HgaI GACGC 1 cut(s) 747
HhaI GCGC 2 cut(s) 643, 1204
Hin1I GRCGYC 1 cut(s) 758
Hin6I GCGC 2 cut(s) 641, 1202
HinP1I GCGC 2 cut(s) 641, 1202
HindIII AAGCTT 2 cut(s) 608, 1452
HinfI GANTC 5 cut(s) 470, 556, 582, 1350, 1399
HpaII CCGG 9 cut(s) 276, 422, 638, 668, 704, 815, 822, 836, 1293
HphI GGTGA 2 cut(s) 257, 524
Hpy166II GTNNAC 4 cut(s) 30, 762, 1156, 1369
Hpy188I TCNGA 2 cut(s) 432, 959
Hpy188III TCNNGA 4 cut(s) 482, 916, 1231, 1270
Hpy8I GTNNAC 4 cut(s) 30, 762, 1156, 1369
HpyAV CCTTC 6 cut(s) 819, 892, 976, 1118, 1144, 1417
HpyCH4III ACNGT 2 cut(s) 256, 1165
HpyCH4IV ACGT 3 cut(s) 65, 408, 1047
HpyF10VI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
HpyF3I CTNAG 4 cut(s) 414, 431, 562, 1322
HpySE526I ACGT 3 cut(s) 65, 408, 1047
Hsp92I GRCGYC 1 cut(s) 758
HspAI GCGC 2 cut(s) 641, 1202
KroI GCCGGC 2 cut(s) 637, 703
KroNI GCCGGC 2 cut(s) 639, 705
Kzo9I GATC 3 cut(s) 1018, 1216, 1383
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 2 cut(s) 461, 1272
Lsp1109I GCAGC 4 cut(s) 329, 553, 694, 1090
LweI GCATC 1 cut(s) 624
MaeI CTAG 1 cut(s) 242
MaeII ACGT 3 cut(s) 65, 408, 1047
MaeIII GTNAC 2 cut(s) 278, 817
MalI GATC 3 cut(s) 1020, 1218, 1385
MbiI CCGCTC 1 cut(s) 1275
MboI GATC 3 cut(s) 1018, 1216, 1383
MfeI CAATTG 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 1172
MlyI GAGTC 2 cut(s) 550, 576
MmeI TCCRAC 3 cut(s) 321, 439, 1244
Mph1103I ATGCAT 1 cut(s) 780
MroNI GCCGGC 2 cut(s) 637, 703
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 3 cut(s) 111, 735, 1456
MslI CAYNNNNRTG 4 cut(s) 383, 504, 632, 1178
MspI CCGG 9 cut(s) 276, 422, 638, 668, 704, 815, 822, 836, 1293
MspR9I CCNGG 7 cut(s) 123, 423, 669, 815, 926, 1055, 1293
MunI CAATTG 1 cut(s) 1009
MvaI CCWGG 3 cut(s) 123, 926, 1055
MwoI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
NaeI GCCGGC 2 cut(s) 639, 705
NciI CCSGG 4 cut(s) 423, 669, 815, 1293
NcoI CCATGG 1 cut(s) 474
NdeII GATC 3 cut(s) 1018, 1216, 1383
NgoMIV GCCGGC 2 cut(s) 637, 703
NlaIV GGNNCC 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 780
NspI RCATGY 1 cut(s) 778
PciSI GCTCTTC 1 cut(s) 353
PdiI GCCGGC 2 cut(s) 639, 705
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 470, 1350, 1399
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 5 cut(s) 319, 543, 709, 877, 1105
PleI GAGTC 2 cut(s) 550, 576
PpsI GAGTC 2 cut(s) 550, 576
Ppu21I YACGTR 1 cut(s) 409
PshAI GACNNNNGTC 1 cut(s) 594
PshBI ATTAAT 1 cut(s) 111
PsiI TTATAA 2 cut(s) 84, 1346
Psp6I CCWGG 3 cut(s) 121, 924, 1053
PspEI GGTNACC 1 cut(s) 817
PspGI CCWGG 3 cut(s) 121, 924, 1053
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 1 cut(s) 1277
PsyI GACNNNGTC 1 cut(s) 953
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 4 cut(s) 383, 504, 632, 1178
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 111, 735, 1456
SatI GCNGC 5 cut(s) 318, 542, 708, 876, 1104
Sau3AI GATC 3 cut(s) 1018, 1216, 1383
Sau96I GGNCC 1 cut(s) 1277
SchI GAGTC 2 cut(s) 550, 576
ScrFI CCNGG 7 cut(s) 123, 423, 669, 815, 926, 1055, 1293
SduI GDGCHC 1 cut(s) 1172
SfaNI GCATC 1 cut(s) 624
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 1277
SmiMI CAYNNNNRTG 4 cut(s) 383, 504, 632, 1178
SsiI CCGC 3 cut(s) 876, 1247, 1275
SspI AATATT 2 cut(s) 664, 1193
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 7 cut(s) 121, 421, 667, 813, 924, 1053, 1291
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 256, 1165
TaiI ACGT 3 cut(s) 68, 411, 1050
TaqI TCGA 6 cut(s) 483, 522, 585, 685, 1230, 1282
TauI GCSGC 1 cut(s) 878
TfiI GAWTC 3 cut(s) 470, 1350, 1399
Tru1I TTAA 3 cut(s) 111, 735, 1456
Tru9I TTAA 3 cut(s) 111, 735, 1456
TscAI CASTG 5 cut(s) 261, 277, 506, 705, 1170
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 4 cut(s) 317, 541, 707, 1103
Tsp45I GTSAC 1 cut(s) 278
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 5 cut(s) 261, 277, 506, 705, 1170
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 1415
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 111
XapI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
XceI RCATGY 1 cut(s) 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 2 cut(s) 29, 761
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 242
Zsp2I ATGCAT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.