RchiOBHm_Chr4g0393831

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000778
Physical Location & Seq
Forward (+)
9387830 .. 9390382
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36637

Sequence Viewer

Length: 1458 bp
ATGGATTACAGTAACCCTTACATTTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTTTTGCATTCCTTTTTTCTGCTTATAAGTCAAAATCCCATGAAAAATCCATTAATCTTCCCCCAGGCAGTTTTGGATGGCCGATTATCGGCGAAACCTTTGCCTTCCTACACAATGACCATAAAAAATTTGTAGGGGATAGAATGAAGAAATACTCGTCCAAAATCTTCAAAACCAAGATATTAGGTGAGCGAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTCCGTTCTTCAAACCAAAAAGCTGCTTCCACCATCATGCCAAGACAAATGGAAAAGCACGTATCTCAGGCACCGGGGTTTCTGAGAGCCGAAGCCCTGGTTGGCTACGTGGCAGCGATGGATTCCATAGTTCTCGACCACCTGAAAAAGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCACCTCACACAGCTACTCGTTTTGACTCTCTCGGCCAAGTTCTTCATGGGACTTGAAGACAGTTGTCGTATTGATAAGCTTGTGGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTTTGAATATTCCGGGGACTGCATTTCATCGAGCAATGAAAGCAGCGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGCGTCTACCGGAAACAAAATACATGATCTTTTGTCTTATATGATTGCTACGCCAGACCCAACCGGAAGGTTCATGCCAGAGAATGAAATTACTGATAAAATTATGGGGAGTGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCTTGAAATTCCTGGGCGAAAGACCTGACATATATGACAAAGTCCGAATTGAACAACTGGAGATTTTGAAGTCTAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTCAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGACCCTACAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTCGGGAGTGGTCCTCGAATTTGCCCCGGAAAGGACTATGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGTCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

485

Amino Acids

54.91

Weight (kDa)

9.27

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 457 1.5e-56 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 87, 1346
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccI GTMKAC 3 cut(s) 32, 764, 1155
AciI CCGC 2 cut(s) 876, 1247
AcoI YGGCCR 3 cut(s) 140, 567, 873
AcsI RAATTY 5 cut(s) 188, 287, 920, 1284, 1317
AcuI CTGAAG 2 cut(s) 1067, 1442
AcyI GRCGYC 1 cut(s) 761
AfiI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
AgsI TTSAA 9 cut(s) 232, 363, 590, 664, 886, 919, 965, 982, 1354
AjnI CCWGG 4 cut(s) 124, 447, 924, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 8 cut(s) 313, 349, 374, 547, 613, 896, 1439, 1454
AluI AGCT 8 cut(s) 313, 349, 374, 547, 613, 896, 1439, 1454
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 2 cut(s) 322, 1436
AoxI GGCC 3 cut(s) 140, 567, 873
ApeKI GCWGC 6 cut(s) 320, 374, 464, 701, 710, 1103
ApoI RAATTY 5 cut(s) 188, 287, 920, 1284, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 3 cut(s) 646, 706, 1204
AspS9I GGNCC 1 cut(s) 1277
AsuC2I CCSGG 3 cut(s) 426, 672, 1293
AsuHPI GGTGA 2 cut(s) 260, 527
AvaII GGWCC 1 cut(s) 1277
BanI GGYRCC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 6 cut(s) 332, 361, 476, 697, 713, 1090
BccI CCATC 9 cut(s) 53, 132, 392, 463, 503, 619, 1140, 1344, 1409
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 4 cut(s) 126, 449, 926, 1055
BcnI CCSGG 3 cut(s) 426, 672, 1293
BcoDI GTCTC 2 cut(s) 322, 1436
BfoI RGCGCY 3 cut(s) 647, 707, 1205
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 7 cut(s) 321, 375, 465, 702, 711, 876, 1104
BlsI GCNGC 7 cut(s) 322, 376, 466, 703, 712, 877, 1105
Bme1390I CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1293
Bme18I GGWCC 1 cut(s) 1277
BmgT120I GGNCC 1 cut(s) 1277
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1293
BmrI ACTGGG 2 cut(s) 514, 1440
BmsI GCATC 1 cut(s) 627
BmuI ACTGGG 2 cut(s) 514, 1440
BoxI GACNNNNGTC 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 3 cut(s) 876, 992, 1076
BpuEI CTTGAG 1 cut(s) 1125
BpuMI CCSGG 3 cut(s) 426, 672, 1293
BsaAI YACGTR 2 cut(s) 412, 460
BsaHI GRCGYC 1 cut(s) 761
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 9 cut(s) 124, 258, 425, 447, 671, 925, 1053, 1123, 1291
BsaWI WCCGGW 2 cut(s) 767, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
Bse118I RCCGGY 2 cut(s) 278, 706
Bse1I ACTGG 5 cut(s) 509, 975, 1163, 1409, 1446
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 4 cut(s) 126, 449, 926, 1055
BseDI CCNNGG 9 cut(s) 124, 258, 425, 447, 671, 925, 1053, 1123, 1291
BseGI GGATG 5 cut(s) 143, 514, 1021, 1151, 1260
BseLI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 2 cut(s) 425, 431
BseNI ACTGG 5 cut(s) 509, 975, 1163, 1409, 1446
BseRI GAGGAG 1 cut(s) 1233
BseXI GCAGC 6 cut(s) 332, 361, 476, 697, 713, 1090
BshFI GGCC 3 cut(s) 142, 569, 875
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 1172
BsiSI CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
BslFI GGGAC 2 cut(s) 597, 688
BslI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
BsmAI GTCTC 2 cut(s) 322, 1436
BsmBI CGTCTC 1 cut(s) 1436
BsmFI GGGAC 2 cut(s) 597, 688
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 3 cut(s) 142, 569, 875
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1172
Bsp143I GATC 2 cut(s) 784, 1383
BspACI CCGC 2 cut(s) 876, 1247
BspANI GGCC 3 cut(s) 142, 569, 875
BspCNI CTCAG 2 cut(s) 426, 430
BspLI GGNNCC 1 cut(s) 423
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 2 cut(s) 278, 706
BsrI ACTGG 5 cut(s) 509, 975, 1163, 1409, 1446
BssAI RCCGGY 2 cut(s) 278, 706
BssECI CCNNGG 9 cut(s) 124, 258, 425, 447, 671, 925, 1053, 1123, 1291
BssMI GATC 2 cut(s) 784, 1383
BssNI GRCGYC 1 cut(s) 761
BssT1I CCWWGG 1 cut(s) 1123
Bst2UI CCWGG 4 cut(s) 126, 449, 926, 1055
Bst4CI ACNGT 4 cut(s) 11, 259, 596, 1165
Bst6I CTCTTC 3 cut(s) 356, 1086, 1203
BstACI GRCGYC 1 cut(s) 761
BstBAI YACGTR 2 cut(s) 412, 460
BstC8I GCNNGC 2 cut(s) 642, 708
BstDEI CTNAG 3 cut(s) 417, 434, 1322
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 5 cut(s) 143, 514, 1021, 1151, 1260
BstH2I RGCGCY 3 cut(s) 647, 707, 1205
BstHHI GCGC 3 cut(s) 646, 706, 1204
BstKTI GATC 2 cut(s) 787, 1386
BstMAI GTCTC 2 cut(s) 322, 1436
BstMBI GATC 2 cut(s) 784, 1383
BstMWI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
BstNI CCWGG 4 cut(s) 126, 449, 926, 1055
BstPAI GACNNNNGTC 1 cut(s) 597
BstSCI CCNGG 7 cut(s) 124, 424, 447, 670, 924, 1053, 1291
BstV1I GCAGC 6 cut(s) 332, 361, 476, 697, 713, 1090
BstV2I GAAGAC 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 640
BsuRI GGCC 3 cut(s) 142, 569, 875
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 2 cut(s) 482, 1076
BtsCI GGATG 5 cut(s) 143, 514, 1021, 1151, 1260
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 502, 1170
Cac8I GCNNGC 2 cut(s) 642, 708
CfoI GCGC 3 cut(s) 646, 706, 1204
Cfr10I RCCGGY 2 cut(s) 278, 706
Cfr13I GGNCC 1 cut(s) 1277
CseI GACGC 1 cut(s) 750
CviAII CATG 8 cut(s) 101, 324, 388, 580, 634, 782, 832, 1180
DdeI CTNAG 3 cut(s) 417, 434, 1322
DpnI GATC 2 cut(s) 786, 1385
DpnII GATC 2 cut(s) 784, 1383
EaeI YGGCCR 3 cut(s) 140, 567, 873
Eam1104I CTCTTC 3 cut(s) 356, 1086, 1203
EarI CTCTTC 3 cut(s) 356, 1086, 1203
EciI GGCGGA 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 1123
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 2 cut(s) 1067, 1442
EcoRII CCWGG 4 cut(s) 124, 447, 924, 1053
EcoT14I CCWWGG 1 cut(s) 1123
ErhI CCWWGG 1 cut(s) 1123
Esp3I CGTCTC 1 cut(s) 1436
FaeI CATG 8 cut(s) 104, 327, 391, 583, 637, 785, 835, 1183
FaqI GGGAC 2 cut(s) 597, 688
FatI CATG 8 cut(s) 100, 323, 387, 579, 633, 781, 831, 1179
FblI GTMKAC 3 cut(s) 32, 764, 1155
Fnu4HI GCNGC 7 cut(s) 321, 375, 465, 702, 711, 876, 1104
FokI GGATG 5 cut(s) 150, 521, 1028, 1158, 1247
Fsp4HI GCNGC 7 cut(s) 321, 375, 465, 702, 711, 876, 1104
GlaI GCGC 3 cut(s) 645, 705, 1203
GluI GCNGC 7 cut(s) 321, 375, 465, 702, 711, 876, 1104
GsuI CTGGAG 3 cut(s) 876, 992, 1076
HaeII RGCGCY 3 cut(s) 647, 707, 1205
HaeIII GGCC 3 cut(s) 142, 569, 875
HapII CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
HgaI GACGC 1 cut(s) 750
HhaI GCGC 3 cut(s) 646, 706, 1204
Hin1I GRCGYC 1 cut(s) 761
Hin1II CATG 8 cut(s) 104, 327, 391, 583, 637, 785, 835, 1183
Hin6I GCGC 3 cut(s) 644, 704, 1202
HinP1I GCGC 3 cut(s) 644, 704, 1202
HindIII AAGCTT 2 cut(s) 611, 1452
HinfI GANTC 4 cut(s) 473, 559, 1350, 1399
HpaII CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
HphI GGTGA 2 cut(s) 260, 527
Hpy166II GTNNAC 4 cut(s) 33, 765, 1156, 1369
Hpy188I TCNGA 2 cut(s) 435, 959
Hpy188III TCNNGA 4 cut(s) 485, 916, 1231, 1270
Hpy8I GTNNAC 4 cut(s) 33, 765, 1156, 1369
HpyAV CCTTC 4 cut(s) 175, 819, 1144, 1417
HpyCH4III ACNGT 4 cut(s) 11, 259, 596, 1165
HpyCH4IV ACGT 3 cut(s) 411, 459, 1047
HpyCH4V TGCA 9 cut(s) 68, 320, 343, 680, 1024, 1106, 1315, 1333, 1380
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
HpyF3I CTNAG 3 cut(s) 417, 434, 1322
HpySE526I ACGT 3 cut(s) 411, 459, 1047
Hsp92I GRCGYC 1 cut(s) 761
Hsp92II CATG 8 cut(s) 104, 327, 391, 583, 637, 785, 835, 1183
HspAI GCGC 3 cut(s) 644, 704, 1202
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
Kzo9I GATC 2 cut(s) 784, 1383
LguI GCTCTTC 1 cut(s) 356
Lsp1109I GCAGC 6 cut(s) 332, 361, 476, 697, 713, 1090
LweI GCATC 1 cut(s) 627
MaeII ACGT 3 cut(s) 411, 459, 1047
MaeIII GTNAC 2 cut(s) 11, 281
MalI GATC 2 cut(s) 786, 1385
MboI GATC 2 cut(s) 784, 1383
MfeI CAATTG 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 1172
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 324
MroNI GCCGGC 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 3 cut(s) 114, 738, 1456
MslI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
MspI CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
MspR9I CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1293
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 4 cut(s) 126, 449, 926, 1055
MwoI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
NaeI GCCGGC 1 cut(s) 708
NciI CCSGG 3 cut(s) 426, 672, 1293
NdeII GATC 2 cut(s) 784, 1383
NgoMIV GCCGGC 1 cut(s) 706
NlaIII CATG 8 cut(s) 104, 327, 391, 583, 637, 785, 835, 1183
NlaIV GGNNCC 1 cut(s) 423
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 356
PctI GAATGC 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 473, 1350, 1399
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 7 cut(s) 322, 376, 466, 703, 712, 877, 1105
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
Ppu21I YACGTR 2 cut(s) 412, 460
PshAI GACNNNNGTC 1 cut(s) 597
PshBI ATTAAT 1 cut(s) 114
PsiI TTATAA 2 cut(s) 87, 1346
Psp6I CCWGG 4 cut(s) 124, 447, 924, 1053
PspGI CCWGG 4 cut(s) 124, 447, 924, 1053
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 1277
PsyI GACNNNGTC 1 cut(s) 953
RseI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 3 cut(s) 114, 738, 1456
SatI GCNGC 7 cut(s) 321, 375, 465, 702, 711, 876, 1104
Sau3AI GATC 2 cut(s) 784, 1383
Sau96I GGNCC 1 cut(s) 1277
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1293
SduI GDGCHC 1 cut(s) 1172
SfaNI GCATC 1 cut(s) 627
SinI GGWCC 1 cut(s) 1277
SmiMI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
SmlI CTYRAG 1 cut(s) 1140
SmoI CTYRAG 1 cut(s) 1140
SsiI CCGC 2 cut(s) 876, 1247
SspI AATATT 2 cut(s) 667, 1193
StyD4I CCNGG 7 cut(s) 124, 424, 447, 670, 924, 1053, 1291
StyI CCWWGG 1 cut(s) 1123
TaaI ACNGT 4 cut(s) 11, 259, 596, 1165
TaiI ACGT 3 cut(s) 414, 462, 1050
TaqI TCGA 5 cut(s) 486, 525, 688, 1230, 1282
TauI GCSGC 1 cut(s) 878
TfiI GAWTC 3 cut(s) 473, 1350, 1399
Tru1I TTAA 3 cut(s) 114, 738, 1456
Tru9I TTAA 3 cut(s) 114, 738, 1456
TscAI CASTG 3 cut(s) 280, 509, 1170
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 6 cut(s) 320, 374, 464, 701, 710, 1103
Tsp45I GTSAC 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 3 cut(s) 280, 509, 1170
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 1415
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 5 cut(s) 188, 287, 920, 1284, 1317
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmiI GTMKAC 3 cut(s) 32, 764, 1155
XmnI GAANNNNTTC 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.