FvH4_3g21260

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001151
Physical Location & Seq
Forward (+)
14247383 .. 14249846
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21260.t1

Sequence Viewer

Length: 1344 bp
ATGCAAAATGTGAAACTGCCAGACCTGCTGGATACATTGGTAAACAAGAGCATGAGCACAGAAGAATGTGAGGCAAACTGCTTGAAGAATTGTTCCTGCATAGCTTATGCTAATTCAGATATTCGTAGTGGAGGCAGTGGCTGTGTTATGTGGTTTGGAAATCTGATGGATATGCGGGAGTTTGTTGAGGATCTTAGTGAGCAAGATATCTTTATTCGGATGCCAGCTTCTGAACTAGCAGCGTCTGTGCTTCTTCTTTTTGGTTTGTTATGTTGGTGTTTAATTCTGAAGCAGAGAGGAAATAAACGGGGTTCAAGAGCTAGTAAGGAAGACTTGGAGTTGCCCCAGTTTGATTTTCATACTATAGCAACTGCCACCGATAGCTTCTCCACCAAAAACAAACTTGGAGAGGGAGGTTTTGGTCCAGTTTACAAGGTCAGACTAACAGAAGAAGAATCAATAGCAGTAAAGAGACTCTCAAAATATTCTGGGCAAGGTGTCGAGGAGTTTAAGAATGGAGTTATGATGATAGCCAATCTTCAACACCGCAACCTGGTTAAGCTTTTGGGCTGCTGCACTGAAGGAGAAGAAAGGATGCTAATCTACGAGTACATGCCCAACAAAAGCTTGGACTGCTTCATCTTTGATCAAAATAGAAAGGTGGTGTTGAGTTGGCAGAAGCGATTTGACATTATCATGGGGATTTCAAGAGGATTACTCTACCTCCACCAGGAGTCCAGAGTAAGAATCATTCACAGGGACCTTAAGAGTAGCAACATCCTACTTGACAATGAGCTAAACCCAAAAATATCCGACTTTGGCATTGCTAGAATTTTTGGAAACAATCAAAGTGAAGCTAAAACCAAACGAGTAATTGGCACATATGGTTATTTGTCTCCTGAGTATGCAATTGATGGCAAGTTTTCAGTGAAATCCGATGTCTTCAGTTTTGGAGTGATTTTGTTAGAAATAATAAGTGGCAGAAAGAACAGAGGGTTTCTTCATCCAGACCACTATCACAGTCTTCTGGGACATGCTTGGCTACTTTGGAACAAAGACAAAGGTTTGGAACTAATAGATCCTTGCTTGGAGGACTCATATGTTGAGTTCGAAGTGCTAAGATGCATTCAAGTGGGCCTTTTGTGCGTTCAAAAGCTGCCAGAAGACAGACCAGAAATGTCATCAGTGCTTCTTATGTTGAGCAATGAGAGTGTAACATTGCCTCAACCGAAGGAGCCTGGTTTCTTCACAGAAAGGGGTTCTATAGACATTGATACCATCATAAGTGATGGAAGAAGTCACACAGGAACCACATTTACAATTACTGTGGTAGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

448

Amino Acids

50.55

Weight (kDa)

5.83

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 1 - 58 1.6e-19 PAN-like domain
Pkinase PF00069 130 - 328 3.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 131 - 330 1.6e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 402 - 447 1e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 33
AciI CCGC 2 cut(s) 175, 547
AclWI GGATC 2 cut(s) 198, 1073
AcsI RAATTY 1 cut(s) 831
AcuI CTGAAG 3 cut(s) 308, 600, 928
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 2 cut(s) 553, 730
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 6 cut(s) 85, 315, 542, 708, 1130, 1151
AjnI CCWGG 3 cut(s) 552, 729, 1237
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 2 cut(s) 466, 900
AlwI GGATC 2 cut(s) 198, 1073
AlwNI CAGNNNCTG 3 cut(s) 141, 230, 245
AoxI GGCC 1 cut(s) 1135
ApeKI GCWGC 4 cut(s) 239, 570, 573, 1156
ApoI RAATTY 1 cut(s) 831
AspS9I GGNCC 3 cut(s) 422, 760, 1135
AsuII TTCGAA 1 cut(s) 1110
AvaII GGWCC 2 cut(s) 422, 760
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BbsI GAAGAC 4 cut(s) 336, 934, 1016, 1170
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 4 cut(s) 251, 557, 560, 1143
BccI CCATC 4 cut(s) 160, 908, 1283, 1286
BciT130I CCWGG 3 cut(s) 554, 731, 1239
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 466, 900
BfaI CTAG 3 cut(s) 236, 321, 828
BfmI CTRYAG 2 cut(s) 363, 1263
BfrI CTTAAG 1 cut(s) 764
BfuAI ACCTGC 1 cut(s) 33
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 4 cut(s) 240, 571, 574, 1157
BlsI GCNGC 4 cut(s) 241, 572, 575, 1158
Bme1390I CCNGG 3 cut(s) 554, 731, 1239
Bme18I GGWCC 2 cut(s) 422, 760
BmgT120I GGNCC 3 cut(s) 422, 760, 1135
BmiI GGNNCC 3 cut(s) 761, 1236, 1309
BmrFI CCNGG 3 cut(s) 554, 731, 1239
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 3 cut(s) 210, 585, 1112
BmuI ACTGGG 1 cut(s) 340
BpiI GAAGAC 4 cut(s) 336, 934, 1016, 1170
BplI GAGNNNNNCTC 2 cut(s) 702, 734
Bpu14I TTCGAA 1 cut(s) 1110
BsaBI GATNNNNATC 1 cut(s) 599
BsaXI ACNNNNNCTCC 2 cut(s) 708, 738
Bsc4I CCNNNNNNNGG 2 cut(s) 553, 730
Bse1I ACTGG 2 cut(s) 346, 425
Bse3DI GCAATG 3 cut(s) 822, 1210, 1217
Bse8I GATNNNNATC 1 cut(s) 599
BseBI CCWGG 3 cut(s) 554, 731, 1239
BseGI GGATG 4 cut(s) 225, 600, 777, 1003
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 2 cut(s) 553, 730
BseMI GCAATG 3 cut(s) 822, 1210, 1217
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 2 cut(s) 346, 425
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 4 cut(s) 251, 557, 560, 1143
BsgI GTGCAG 1 cut(s) 559
BshFI GGCC 1 cut(s) 1137
BsiHKAI GWGCWC 1 cut(s) 59
BslFI GGGAC 2 cut(s) 773, 1044
BslI CCNNNNNNNGG 2 cut(s) 553, 730
BsmAI GTCTC 2 cut(s) 466, 900
BsmFI GGGAC 2 cut(s) 773, 1044
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 1 cut(s) 1137
Bsp119I TTCGAA 1 cut(s) 1110
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 3 cut(s) 190, 646, 1078
BspACI CCGC 2 cut(s) 175, 547
BspANI GGCC 1 cut(s) 1137
BspCNI CTCAG 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 761, 1236, 1309
BspMI ACCTGC 1 cut(s) 33
BspPI GGATC 2 cut(s) 198, 1073
BspT104I TTCGAA 1 cut(s) 1110
BspTI CTTAAG 1 cut(s) 764
BsrDI GCAATG 3 cut(s) 822, 1210, 1217
BsrI ACTGG 2 cut(s) 346, 425
BssMI GATC 3 cut(s) 190, 646, 1078
Bst2UI CCWGG 3 cut(s) 554, 731, 1239
Bst4CI ACNGT 2 cut(s) 1022, 1327
BstAFI CTTAAG 1 cut(s) 764
BstBI TTCGAA 1 cut(s) 1110
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 3 cut(s) 194, 900, 1118
BstENI CCTNNNNNAGG 1 cut(s) 728
BstF5I GGATG 4 cut(s) 225, 600, 777, 1003
BstKTI GATC 3 cut(s) 193, 649, 1081
BstMAI GTCTC 2 cut(s) 466, 900
BstMBI GATC 3 cut(s) 190, 646, 1078
BstMWI GCNNNNNNNGC 3 cut(s) 25, 633, 1143
BstNI CCWGG 3 cut(s) 554, 731, 1239
BstNSI RCATGY 2 cut(s) 616, 1037
BstSCI CCNGG 3 cut(s) 552, 729, 1237
BstSFI CTRYAG 2 cut(s) 363, 1263
BstV1I GCAGC 4 cut(s) 251, 557, 560, 1143
BstV2I GAAGAC 4 cut(s) 336, 934, 1016, 1170
BstX2I RGATCY 2 cut(s) 190, 1078
BstYI RGATCY 2 cut(s) 190, 1078
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 1 cut(s) 1137
BtsCI GGATG 4 cut(s) 225, 600, 777, 1003
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 4 cut(s) 142, 576, 933, 1191
BveI ACCTGC 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 225
CaiI CAGNNNCTG 3 cut(s) 141, 230, 245
Cfr13I GGNCC 3 cut(s) 422, 760, 1135
CseI GACGC 1 cut(s) 231
CsiI ACCWGGT 1 cut(s) 552
Csp6I GTAC 1 cut(s) 610
CspCI CAANNNNNGTGG 2 cut(s) 1308, 1343
CviAII CATG 4 cut(s) 52, 613, 697, 1034
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 3 cut(s) 194, 900, 1118
DpnI GATC 3 cut(s) 192, 648, 1080
DpnII GATC 3 cut(s) 190, 646, 1078
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 422, 760
Eco57I CTGAAG 3 cut(s) 308, 600, 928
EcoNI CCTNNNNNAGG 1 cut(s) 728
EcoO109I RGGNCCY 1 cut(s) 760
EcoRII CCWGG 3 cut(s) 552, 729, 1237
EcoRV GATATC 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 1127
FaeI CATG 4 cut(s) 55, 616, 700, 1037
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 1122, 1154
FaqI GGGAC 2 cut(s) 773, 1044
FatI CATG 4 cut(s) 51, 612, 696, 1033
FauI CCCGC 1 cut(s) 168
FauNDI CATATG 2 cut(s) 883, 1099
FbaI TGATCA 1 cut(s) 646
Fnu4HI GCNGC 4 cut(s) 240, 571, 574, 1157
FokI GGATG 4 cut(s) 232, 607, 764, 990
Fsp4HI GCNGC 4 cut(s) 240, 571, 574, 1157
FspBI CTAG 3 cut(s) 236, 321, 828
GluI GCNGC 4 cut(s) 240, 571, 574, 1157
HaeIII GGCC 1 cut(s) 1137
HgaI GACGC 1 cut(s) 231
Hin1II CATG 4 cut(s) 55, 616, 700, 1037
HindIII AAGCTT 2 cut(s) 560, 625
HinfI GANTC 5 cut(s) 455, 474, 734, 747, 1094
Hpy166II GTNNAC 2 cut(s) 43, 430
Hpy188I TCNGA 8 cut(s) 118, 165, 219, 232, 288, 440, 814, 937
Hpy188III TCNNGA 5 cut(s) 315, 708, 738, 899, 1007
Hpy8I GTNNAC 2 cut(s) 43, 430
HpyAV CCTTC 2 cut(s) 575, 1225
HpyCH4III ACNGT 2 cut(s) 1022, 1327
HpyCH4V TGCA 5 cut(s) 4, 99, 576, 908, 1125
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 633, 1143
HpyF3I CTNAG 3 cut(s) 194, 900, 1118
Hsp92II CATG 4 cut(s) 55, 616, 700, 1037
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 3 cut(s) 190, 646, 1078
LmnI GCTCC 1 cut(s) 1234
Lsp1109I GCAGC 4 cut(s) 251, 557, 560, 1143
LweI GCATC 3 cut(s) 210, 585, 1112
MabI ACCWGGT 1 cut(s) 552
MaeI CTAG 3 cut(s) 236, 321, 828
MaeIII GTNAC 2 cut(s) 1213, 1298
MalI GATC 3 cut(s) 192, 648, 1080
MboI GATC 3 cut(s) 190, 646, 1078
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 2 cut(s) 190, 1078
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 7 cut(s) 88, 112, 282, 831, 873, 909, 1320
MlyI GAGTC 3 cut(s) 468, 743, 1088
MmeI TCCRAC 1 cut(s) 837
Mph1103I ATGCAT 1 cut(s) 1127
MseI TTAA 4 cut(s) 281, 510, 558, 765
MslI CAYNNNNRTG 2 cut(s) 695, 1130
MspCI CTTAAG 1 cut(s) 764
MspR9I CCNGG 3 cut(s) 554, 731, 1239
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 3 cut(s) 554, 731, 1239
MwoI GCNNNNNNNGC 3 cut(s) 25, 633, 1143
NdeI CATATG 2 cut(s) 883, 1099
NdeII GATC 3 cut(s) 190, 646, 1078
NlaIII CATG 4 cut(s) 55, 616, 700, 1037
NlaIV GGNNCC 3 cut(s) 761, 1236, 1309
NmuCI GTSAC 1 cut(s) 1298
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 2 cut(s) 616, 1037
NspV TTCGAA 1 cut(s) 1110
PctI GAATGC 1 cut(s) 1125
PfeI GAWTC 2 cut(s) 455, 747
PkrI GCNGC 4 cut(s) 241, 572, 575, 1158
PleI GAGTC 3 cut(s) 468, 742, 1088
PpsI GAGTC 3 cut(s) 468, 742, 1088
PpuMI RGGWCCY 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 3 cut(s) 552, 729, 1237
PspGI CCWGG 3 cut(s) 552, 729, 1237
PspN4I GGNNCC 3 cut(s) 761, 1236, 1309
PspPI GGNCC 3 cut(s) 422, 760, 1135
PspPPI RGGWCCY 1 cut(s) 760
PsrI GAACNNNNNNTAC 2 cut(s) 1300, 1332
PstNI CAGNNNCTG 3 cut(s) 141, 230, 245
PsuI RGATCY 2 cut(s) 190, 1078
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
RseI CAYNNNNRTG 2 cut(s) 695, 1130
SaqAI TTAA 4 cut(s) 281, 510, 558, 765
SatI GCNGC 4 cut(s) 240, 571, 574, 1157
Sau3AI GATC 3 cut(s) 190, 646, 1078
Sau96I GGNCC 3 cut(s) 422, 760, 1135
SchI GAGTC 3 cut(s) 468, 743, 1088
ScrFI CCNGG 3 cut(s) 554, 731, 1239
SduI GDGCHC 1 cut(s) 59
SexAI ACCWGGT 1 cut(s) 552
SfaNI GCATC 3 cut(s) 210, 585, 1112
SfcI CTRYAG 2 cut(s) 363, 1263
SfuI TTCGAA 1 cut(s) 1110
SinI GGWCC 2 cut(s) 422, 760
SmiMI CAYNNNNRTG 2 cut(s) 695, 1130
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 7 cut(s) 88, 112, 282, 831, 873, 909, 1320
SsiI CCGC 2 cut(s) 175, 547
SspI AATATT 1 cut(s) 485
SspMI CTAG 3 cut(s) 236, 321, 828
StyD4I CCNGG 3 cut(s) 552, 729, 1237
TaaI ACNGT 2 cut(s) 1022, 1327
TaqI TCGA 3 cut(s) 501, 1110, 1339
TasI AATT 7 cut(s) 88, 112, 282, 831, 873, 909, 1320
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 2 cut(s) 455, 747
Tru1I TTAA 4 cut(s) 281, 510, 558, 765
Tru9I TTAA 4 cut(s) 281, 510, 558, 765
TscAI CASTG 4 cut(s) 142, 583, 933, 1191
TseFI GTSAC 1 cut(s) 1298
TseI GCWGC 4 cut(s) 239, 570, 573, 1156
Tsp45I GTSAC 1 cut(s) 1298
TspDTI ATGAA 3 cut(s) 347, 628, 992
TspRI CASTG 4 cut(s) 142, 583, 933, 1191
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 422, 760
XagI CCTNNNNNAGG 1 cut(s) 728
XapI RAATTY 1 cut(s) 831
XceI RCATGY 2 cut(s) 616, 1037
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XspI CTAG 3 cut(s) 236, 321, 828
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.