Rh5BG400000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001151
Physical Location & Seq
Reverse (-)
64950864 .. 64952667
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG400000.1

Sequence Viewer

Length: 1440 bp
ATGAAGGGCCTCATCTTCATCTTCATCTCTCTATTCCTATTTTCAGCCTTGAATTTCTCCATTGCAGCTGATACCATATCTCCATCACAATCCATCACCGGCTCGAACACCCTGGTTTCTTCCAACCAGAGATTTGTACTAGGACTCTTCTCCCCACCCAATTCTAGAGCATGGTACTTAGGACTATGGTACAAGAAATTTCCAGAGGTTGTTGTATGGATTGCAAACAGAGAAAACCCACTTCCGGGTTCAAATGGAGCCTTGACATTATCAGAAAAAGGCACCCTCATCCTTTCAGACCAAATGAACAAGACAATTTGGTTCTCAAATTCTTCCAGAGTAGCCGAAAACCCAATTGCGCAGCTTTTGGTGACGGGAAATCTTGTTGTCAGAGACCAGGCGTCTCCAACAGCAGCAGCAGAGTCCGAAAGCTATGTTTTTCAAAGCTTTGATTTCCCATCAAACACGCTTTTACCAGATATGAAGGTGGGGTGGGACTTCAGAACTGGCCAAAACCATTTCTTGACTAGTTGGAAAAATGCTAGCGATCCCTCCCCTGGAGAATATACCTATAGGATTGAAAACCTTGAGTTGCCTCAGCTTGTTGTCGCTCAAGGATCAAAGAAAGTGTTCAGGACAGGGCCCTGGAATGGTCTTCGATTTACAGGTACTCCTGTTTCGGTTGTTACAGCAGCATCATTTTTGGTACCAATCTTTGTCTACAATACCAAGGAGTTGTACTACTCATATGAGGTTGCTGATGATTCTATTCTGACAAGAGTGACGCTGACTGAGTCGGGTTTGGCTCAGCGGCTAGTGATGAATGAAGGGAGCAGTGAATGGGCTGTTATGTACACTCTGCAGAACAATCAGTGTGATGATTATGGTCAGTGTGGGCCAAATGGCATCTGCAAGATTTACAAATCACCCATTTGTGAATGCTTGCAGGGGTTTGTTCCCAAATCTCCAAAGGAATGGGAGTTTCTTAACTGGACAACCGGGTGCACGAGGAGGACAGCACTGCATTGTGAGAGAGGGGAAGGATTTGTGAAAGTTAATAATGTGAAACTGCCGGACTTGTTGGATTTTCTGGTCAACAAAACCATGCTCCGCGGAGAGGAATGTGAGGCAGAGTGTTTGAGGAACTGCTCCTGTATGGCTTATGCCAATTCGAACATAACCAGTGGAGGCAAAGGATGTCTTATGTGGTTTGGCAAGCTGATTGATATGCGGGAGATCGTTGAAGATAGTGACCAGATATATATTCGGATGCCAGCTTCCGAACTAGGTGATACCAGCCAGAAACATAAAAGAGTATTGCTCGTCTCGGTCATATCATCAGCAGGTTTTGTGCTTCTTTTTCTGGGTTTCTCATGCTGCTATATTTATGTGAAGAAGAGGGAAAAGAAAATGGGTAAGCTTGCATATTTCCCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

479

Amino Acids

53.59

Weight (kDa)

7.81

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 179 2.6e-32 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 4.1e-28 S-locus glycoprotein domain
PAN_2 PF08276 343 - 410 2.9e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 1334
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 281, 706
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 1113
AciI CCGC 4 cut(s) 811, 1111, 1113, 1231
AclWI GGATC 2 cut(s) 542, 625
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 52, 197, 328
AcuI CTGAAG 1 cut(s) 484
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 7 cut(s) 138, 176, 191, 670, 708, 740, 854
AfiI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
AgsI TTSAA 5 cut(s) 52, 252, 443, 581, 1244
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 4 cut(s) 111, 396, 556, 644
AluBI AGCT 8 cut(s) 68, 364, 432, 447, 601, 1219, 1277, 1420
AluI AGCT 8 cut(s) 68, 364, 432, 447, 601, 1219, 1277, 1420
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 3 cut(s) 387, 408, 1330
Alw44I GTGCAC 1 cut(s) 1003
AlwI GGATC 2 cut(s) 542, 625
AoxI GGCC 4 cut(s) 7, 508, 641, 896
ApaI GGGCCC 1 cut(s) 645
ApaLI GTGCAC 1 cut(s) 1003
ApeKI GCWGC 6 cut(s) 65, 361, 413, 416, 692, 1377
ApoI RAATTY 3 cut(s) 52, 197, 328
Asp700I GAANNNNTTC 1 cut(s) 629
Asp718I GGTACC 1 cut(s) 706
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 4 cut(s) 7, 641, 642, 896
AsuC2I CCSGG 2 cut(s) 246, 1000
AsuHPI GGTGA 4 cut(s) 88, 382, 918, 1301
AsuII TTCGAA 1 cut(s) 1172
AsuNHI GCTAGC 1 cut(s) 542
BaeGI GKGCMC 2 cut(s) 645, 1007
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 2 cut(s) 281, 706
BanII GRGCYC 1 cut(s) 645
BauI CACGAG 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 647
Bbv12I GWGCWC 1 cut(s) 1007
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 6 cut(s) 77, 373, 425, 428, 704, 1364
BccI CCATC 3 cut(s) 91, 101, 466
BciT130I CCWGG 4 cut(s) 113, 398, 558, 646
BcnI CCSGG 2 cut(s) 246, 1000
BcoDI GTCTC 3 cut(s) 387, 408, 1330
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 7 cut(s) 140, 165, 528, 543, 815, 1286, 1438
BfmI CTRYAG 2 cut(s) 571, 860
BfuAI ACCTGC 1 cut(s) 1334
BisI GCNGC 7 cut(s) 66, 362, 414, 417, 693, 812, 1378
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 7 cut(s) 67, 363, 415, 418, 694, 813, 1379
Bme1390I CCNGG 6 cut(s) 113, 246, 398, 558, 646, 1000
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 4 cut(s) 7, 641, 642, 896
BmiI GGNNCC 4 cut(s) 259, 283, 643, 708
BmrFI CCNGG 6 cut(s) 113, 246, 398, 558, 646, 1000
BmsI GCATC 3 cut(s) 704, 915, 1260
BmtI GCTAGC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 647
BplI GAGNNNNNCTC 2 cut(s) 1305, 1337
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 1172
BpuEI CTTGAG 2 cut(s) 597, 608
BpuMI CCSGG 2 cut(s) 246, 1000
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 655, 685
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 511, 995, 1182
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 4 cut(s) 113, 398, 558, 646
BseDI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BseGI GGATG 3 cut(s) 288, 1202, 1275
BseLI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 3 cut(s) 611, 783, 821
BseNI ACTGG 3 cut(s) 511, 995, 1182
BseRI GAGGAG 1 cut(s) 1024
BseSI GKGCMC 2 cut(s) 645, 1007
BseXI GCAGC 6 cut(s) 77, 373, 425, 428, 704, 1364
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 4 cut(s) 9, 510, 643, 898
BshNI GGYRCC 2 cut(s) 281, 706
BsiHKAI GWGCWC 1 cut(s) 1007
BsiSI CCGG 4 cut(s) 99, 245, 999, 1073
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
BsmAI GTCTC 3 cut(s) 387, 408, 1330
BsmBI CGTCTC 2 cut(s) 408, 1330
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 4 cut(s) 9, 510, 643, 898
Bso31I GGTCTC 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 1172
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 645, 1007
Bsp1407I TGTACA 1 cut(s) 852
Bsp143I GATC 3 cut(s) 547, 617, 1236
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 4 cut(s) 811, 1111, 1113, 1231
BspANI GGCC 4 cut(s) 9, 510, 643, 898
BspCNI CTCAG 3 cut(s) 610, 784, 820
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 4 cut(s) 259, 283, 643, 708
BspMAI CTGCAG 1 cut(s) 864
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 542, 625
BspT104I TTCGAA 1 cut(s) 1172
BspT107I GGYRCC 2 cut(s) 281, 706
BspTNI GGTCTC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 852
BsrI ACTGG 3 cut(s) 511, 995, 1182
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BssMI GATC 3 cut(s) 547, 617, 1236
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 1006
BssT1I CCWWGG 1 cut(s) 729
Bst2BI CACGAG 1 cut(s) 1006
Bst2UI CCWGG 4 cut(s) 113, 398, 558, 646
Bst6I CTCTTC 2 cut(s) 152, 1391
BstACI GRCGYC 1 cut(s) 401
BstAUI TGTACA 1 cut(s) 852
BstBI TTCGAA 1 cut(s) 1172
BstC8I GCNNGC 5 cut(s) 544, 944, 1217, 1275, 1422
BstDEI CTNAG 4 cut(s) 178, 597, 792, 807
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 3 cut(s) 288, 1202, 1275
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 550, 620, 1239
BstMAI GTCTC 3 cut(s) 387, 408, 1330
BstMBI GATC 3 cut(s) 547, 617, 1236
BstNI CCWGG 4 cut(s) 113, 398, 558, 646
BstSCI CCNGG 6 cut(s) 111, 244, 396, 556, 644, 998
BstSFI CTRYAG 2 cut(s) 571, 860
BstSLI GKGCMC 2 cut(s) 645, 1007
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 6 cut(s) 77, 373, 425, 428, 704, 1364
BstV2I GAAGAC 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 975
BsuRI GGCC 4 cut(s) 9, 510, 643, 898
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 3 cut(s) 288, 1202, 1275
BtsI GCAGTG 2 cut(s) 841, 1019
BtsIMutI CAGTG 5 cut(s) 841, 878, 896, 1019, 1189
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 5 cut(s) 544, 944, 1217, 1275, 1422
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 7, 641, 642, 896
Cfr42I CCGCGG 1 cut(s) 1114
CseI GACGC 2 cut(s) 390, 793
Csp6I GTAC 7 cut(s) 137, 175, 190, 669, 707, 739, 853
CviAII CATG 3 cut(s) 171, 1105, 1374
CviQI GTAC 7 cut(s) 137, 175, 190, 669, 707, 739, 853
DdeI CTNAG 4 cut(s) 178, 597, 792, 807
DpnI GATC 3 cut(s) 549, 619, 1238
DpnII GATC 3 cut(s) 547, 617, 1236
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 2 cut(s) 152, 1391
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 2 cut(s) 152, 1391
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 484
EcoO109I RGGNCCY 3 cut(s) 7, 641, 642
EcoRII CCWGG 4 cut(s) 111, 396, 556, 644
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 729
Esp3I CGTCTC 2 cut(s) 408, 1330
FaeI CATG 3 cut(s) 174, 1108, 1377
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 170, 1104, 1373
FauI CCCGC 1 cut(s) 1224
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 66, 362, 414, 417, 693, 812, 1378
FokI GGATG 3 cut(s) 275, 1209, 1282
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 7 cut(s) 66, 362, 414, 417, 693, 812, 1378
FspBI CTAG 7 cut(s) 140, 165, 528, 543, 815, 1286, 1438
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GluI GCNGC 7 cut(s) 66, 362, 414, 417, 693, 812, 1378
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 4 cut(s) 9, 510, 643, 898
HapII CCGG 4 cut(s) 99, 245, 999, 1073
HgaI GACGC 2 cut(s) 390, 793
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 3 cut(s) 174, 1108, 1377
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 2 cut(s) 445, 1418
HinfI GANTC 4 cut(s) 144, 422, 764, 794
HpaII CCGG 4 cut(s) 99, 245, 999, 1073
HphI GGTGA 4 cut(s) 88, 382, 918, 1301
Hpy166II GTNNAC 4 cut(s) 721, 855, 1005, 1096
Hpy188I TCNGA 8 cut(s) 274, 298, 392, 427, 503, 774, 1269, 1282
Hpy188III TCNNGA 5 cut(s) 165, 203, 336, 523, 634
Hpy8I GTNNAC 4 cut(s) 721, 855, 1005, 1096
HpyAV CCTTC 3 cut(s) 478, 821, 1034
HpyCH4V TGCA 8 cut(s) 65, 224, 862, 912, 946, 1005, 1024, 1424
HpyF3I CTNAG 4 cut(s) 178, 597, 792, 807
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 3 cut(s) 174, 1108, 1377
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 710
KspI CCGCGG 1 cut(s) 1114
Kzo9I GATC 3 cut(s) 547, 617, 1236
LmnI GCTCC 4 cut(s) 257, 831, 1113, 1154
Lsp1109I GCAGC 6 cut(s) 77, 373, 425, 428, 704, 1364
LweI GCATC 3 cut(s) 704, 915, 1260
MaeI CTAG 7 cut(s) 140, 165, 528, 543, 815, 1286, 1438
MaeIII GTNAC 4 cut(s) 370, 685, 781, 1250
MalI GATC 3 cut(s) 549, 619, 1238
MboI GATC 3 cut(s) 547, 617, 1236
MboII GAAGA 9 cut(s) 7, 13, 111, 139, 324, 647, 1256, 1405, 1408
MfeI CAATTG 1 cut(s) 354
MhlI GDGCHC 2 cut(s) 645, 1007
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 7 cut(s) 52, 160, 197, 315, 328, 354, 1168
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 3 cut(s) 138, 431, 803
MmeI TCCRAC 4 cut(s) 147, 431, 512, 1062
Mox20I TGGCCA 1 cut(s) 510
MroXI GAANNNNTTC 1 cut(s) 629
MscI TGGCCA 1 cut(s) 510
MseI TTAA 2 cut(s) 987, 1056
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 68, 811, 1113
MspI CCGG 4 cut(s) 99, 245, 999, 1073
MspR9I CCNGG 6 cut(s) 113, 246, 398, 558, 646, 1000
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 4 cut(s) 113, 398, 558, 646
MvnI CGCG 1 cut(s) 1113
NciI CCSGG 2 cut(s) 246, 1000
NdeI CATATG 1 cut(s) 748
NdeII GATC 3 cut(s) 547, 617, 1236
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 3 cut(s) 174, 1108, 1377
NlaIV GGNNCC 4 cut(s) 259, 283, 643, 708
NmuCI GTSAC 3 cut(s) 370, 781, 1250
NsbI TGCGCA 1 cut(s) 360
NspV TTCGAA 1 cut(s) 1172
PctI GAATGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 764
PflFI GACNNNGTC 1 cut(s) 793
PkrI GCNGC 7 cut(s) 67, 363, 415, 418, 694, 813, 1379
PleI GAGTC 3 cut(s) 138, 430, 802
PpsI GAGTC 3 cut(s) 138, 430, 802
Psp6I CCWGG 4 cut(s) 111, 396, 556, 644
PspGI CCWGG 4 cut(s) 111, 396, 556, 644
PspN4I GGNNCC 4 cut(s) 259, 283, 643, 708
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 4 cut(s) 7, 641, 642, 896
PstI CTGCAG 1 cut(s) 864
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 7 cut(s) 138, 176, 191, 670, 708, 740, 854
RsaNI GTAC 7 cut(s) 137, 175, 190, 669, 707, 739, 853
SacII CCGCGG 1 cut(s) 1114
SaqAI TTAA 2 cut(s) 987, 1056
SatI GCNGC 7 cut(s) 66, 362, 414, 417, 693, 812, 1378
Sau3AI GATC 3 cut(s) 547, 617, 1236
Sau96I GGNCC 4 cut(s) 7, 641, 642, 896
SchI GAGTC 3 cut(s) 138, 431, 803
ScrFI CCNGG 6 cut(s) 113, 246, 398, 558, 646, 1000
SduI GDGCHC 2 cut(s) 645, 1007
SfaNI GCATC 3 cut(s) 704, 915, 1260
SfcI CTRYAG 2 cut(s) 571, 860
Sfr303I CCGCGG 1 cut(s) 1114
SfuI TTCGAA 1 cut(s) 1172
SgrBI CCGCGG 1 cut(s) 1114
SmlI CTYRAG 2 cut(s) 587, 612
SmoI CTYRAG 2 cut(s) 587, 612
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 7 cut(s) 52, 160, 197, 315, 328, 354, 1168
SsiI CCGC 4 cut(s) 811, 1111, 1113, 1231
SspMI CTAG 7 cut(s) 140, 165, 528, 543, 815, 1286, 1438
StyD4I CCNGG 6 cut(s) 111, 244, 396, 556, 644, 998
StyI CCWWGG 1 cut(s) 729
TaqI TCGA 3 cut(s) 104, 658, 1172
TaqII GACCGA 1 cut(s) 1318
TasI AATT 7 cut(s) 52, 160, 197, 315, 328, 354, 1168
TatI WGTACW 3 cut(s) 136, 738, 852
TauI GCSGC 1 cut(s) 814
TfiI GAWTC 1 cut(s) 764
Tru1I TTAA 2 cut(s) 987, 1056
Tru9I TTAA 2 cut(s) 987, 1056
TscAI CASTG 5 cut(s) 841, 878, 896, 1026, 1189
TseFI GTSAC 3 cut(s) 370, 781, 1250
TseI GCWGC 6 cut(s) 65, 361, 413, 416, 692, 1377
Tsp45I GTSAC 3 cut(s) 370, 781, 1250
TspDTI ATGAA 7 cut(s) 7, 13, 17, 320, 497, 836, 840
TspRI CASTG 5 cut(s) 841, 878, 896, 1026, 1189
Tth111I GACNNNGTC 1 cut(s) 793
VneI GTGCAC 1 cut(s) 1003
XapI RAATTY 3 cut(s) 52, 197, 328
XbaI TCTAGA 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 7 cut(s) 140, 165, 528, 543, 815, 1286, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.