FvH4_3g21271

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001151
Physical Location & Seq
Forward (+)
14244420 .. 14247247
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21271.t1

Sequence Viewer

Length: 1314 bp
ATGGGGCAAATCGTAATTAACCGGGCATTTCTGTCAAAACGGCAAAAGCGACCCTTTTTCTTCTTCATTCTCATTTACCGATCAGTCAATCCTCCTTCTTACGATGGACCAGCAGTCAATCCCACTGATGCTGCCAACGTGGCAACCCGCCATCCGTCAGGATCCTGGCATTCATCTTCCTCACGGTGGCGATTGGACGGAAAATCGCTGCATGGCCGCCACCTCTGGCTTCCACAAACAGTTTTGGCGGGAAATTACCCTCAAGAAATTCAGCAATCTTCAAAGGCTGTTTCTGAGGTGGAGAAGTTCCGGCTAAGTCAAAAATGGTGGCTCCTGTTTCAGATTTCCGCACCCGATAGAGGAAAGTCCAAGTTTTTGCTGCCGTTTCACAGCTCCTTCCACAATTCAAATCCACAACTTTGTTGCTGGTGCTGTCTTCCGAGAACAAGATTTTCCTGCAGACTTGGAAAACGTGACACTGATACAATATATCCATCACAGTCCATAACGGGCTCTGGTACCTTGGTTTCTTCCGGCGCAAGCTTTGAATTGGGACTGTTCTCCCCCGGAAACTCTGGAGCCTGGTACTTGGGAATTTGGTACAAGAAATTTCCTGATATTGTAGTATGGGTTGCCAATAGAGAAAATCCAATCCCTGGATCACATGGAGCCTTGAGGTTGTCCAAAAATGGCAGTCTAGTCATTTTAGATCAGATGAACAACACCATTTGGTCTTCTAATTCTTCCAGAGTAGCAGAAGATCCTGTTGCACAACTTTTGGAGAGTGGAAATCTTGTTGTTAGAGACAGTGCTGCCACAGGTTCTGAAAACTATGTGTGGCAAAGTTTTGATTTCCCATCAGACACATTGTTACCGGGGATGAAGCTGGGGTGGACCTTCAAAAGGGGCCTCAGCCAATTCTTGACTTCATGGAAAAATGCTAGTCATCCATCTCTTGGGGCATATACTTATGGCATGGATAATGATGTGGTGCCTCAGTTTGTTTTTGCCAAAGGGTCAAGCAAAATCTTCCGTACTGGACCTTGGAATGGTGTTCGATTTCCTGGCACTTTTGTTTCCAACCGTAATTTCACAGTTTTAAAACCAAATTTTGTGTACAATAGCAATGAGATATATTACACGTTTGATGCTGCTGAAAGCTCTATTACTACAAGACTACAGCTGTCTGAATCAGGTCAGATGCTGCGGCTAGTGCTGAATAAAGGAAGCACAGAATGGGCCCTCATGTATACATTGCAGAATGATCAATGTAACAATTATGGAGTGTGGAGCAAATGGAATTTGCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

438

Amino Acids

49.26

Weight (kDa)

9.62

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 208 - 312 4.2e-37 D-mannose binding lectin
S_locus_glycop PF00954 344 - 429 3e-12 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 518
AccB1I GGYRCC 2 cut(s) 518, 991
AccB7I CCANNNNNTGG 2 cut(s) 656, 956
AccI GTMKAC 1 cut(s) 1250
AciI CCGC 5 cut(s) 148, 217, 248, 348, 1207
AclWI GGATC 4 cut(s) 156, 169, 667, 755
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 5 cut(s) 267, 594, 608, 1108, 1300
AfaI GTAC 5 cut(s) 520, 587, 602, 1036, 1118
AfiI CCNNNNNNNGG 6 cut(s) 186, 359, 656, 903, 956, 1049
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 4 cut(s) 282, 408, 548, 901
AhdI GACNNNNNGTC 1 cut(s) 113
AjnI CCWGG 4 cut(s) 164, 581, 655, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AluBI AGCT 5 cut(s) 393, 543, 886, 1161, 1183
AluI AGCT 5 cut(s) 393, 543, 886, 1161, 1183
Alw26I GTCTC 1 cut(s) 798
AlwI GGATC 4 cut(s) 156, 169, 667, 755
AlwNI CAGNNNCTG 2 cut(s) 824, 1204
AoxI GGCC 3 cut(s) 214, 907, 1239
ApaI GGGCCC 1 cut(s) 1243
ApeKI GCWGC 6 cut(s) 131, 208, 379, 812, 1151, 1204
ApoI RAATTY 5 cut(s) 267, 594, 608, 1108, 1300
Asp718I GGTACC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 6 cut(s) 107, 894, 907, 1040, 1239, 1240
AsuC2I CCSGG 3 cut(s) 23, 567, 876
AvaII GGWCC 3 cut(s) 107, 894, 1040
BaeGI GKGCMC 1 cut(s) 1243
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BamHI GGATCC 1 cut(s) 161
BanI GGYRCC 2 cut(s) 518, 991
BanII GRGCYC 2 cut(s) 515, 1243
BbsI GAAGAC 2 cut(s) 428, 726
BbvCI CCTCAGC 1 cut(s) 911
BbvI GCAGC 6 cut(s) 118, 195, 366, 799, 1138, 1191
BccI CCATC 5 cut(s) 98, 159, 502, 865, 958
BceAI ACGGC 2 cut(s) 56, 367
BciT130I CCWGG 4 cut(s) 166, 583, 657, 1065
BclI TGATCA 1 cut(s) 1264
BcnI CCSGG 3 cut(s) 23, 567, 876
BcoDI GTCTC 1 cut(s) 798
BfaI CTAG 3 cut(s) 698, 942, 1211
BfmI CTRYAG 2 cut(s) 457, 1178
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 8 cut(s) 132, 209, 217, 380, 813, 1152, 1205, 1208
BlsI GCNGC 8 cut(s) 133, 210, 218, 381, 814, 1153, 1206, 1209
Bme1390I CCNGG 7 cut(s) 23, 166, 567, 583, 657, 876, 1065
Bme18I GGWCC 3 cut(s) 107, 894, 1040
BmeRI GACNNNNNGTC 1 cut(s) 113
BmgT120I GGNCC 6 cut(s) 107, 894, 907, 1040, 1239, 1240
BmiI GGNNCC 8 cut(s) 163, 332, 520, 580, 670, 908, 993, 1241
BmrFI CCNGG 7 cut(s) 23, 166, 567, 583, 657, 876, 1065
BmsI GCATC 3 cut(s) 118, 1138, 1191
BpiI GAAGAC 2 cut(s) 428, 726
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 911
BpuEI CTTGAG 2 cut(s) 246, 694
BpuMI CCSGG 3 cut(s) 23, 567, 876
BsaJI CCNNGG 5 cut(s) 522, 565, 655, 875, 1043
Bsc4I CCNNNNNNNGG 6 cut(s) 186, 359, 656, 903, 956, 1049
Bse1I ACTGG 1 cut(s) 1042
Bse3DI GCAATG 2 cut(s) 1132, 1253
BseBI CCWGG 4 cut(s) 166, 583, 657, 1065
BseDI CCNNGG 5 cut(s) 522, 565, 655, 875, 1043
BseGI GGATG 3 cut(s) 151, 885, 946
BseLI CCNNNNNNNGG 6 cut(s) 186, 359, 656, 903, 956, 1049
BseMI GCAATG 2 cut(s) 1132, 1253
BseMII CTCAG 3 cut(s) 285, 925, 1010
BseNI ACTGG 1 cut(s) 1042
BseSI GKGCMC 1 cut(s) 1243
BseXI GCAGC 6 cut(s) 118, 195, 366, 799, 1138, 1191
BseYI CCCAGC 1 cut(s) 886
BshFI GGCC 3 cut(s) 216, 909, 1241
BshNI GGYRCC 2 cut(s) 518, 991
BsiSI CCGG 5 cut(s) 22, 310, 534, 567, 875
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 6 cut(s) 186, 359, 656, 903, 956, 1049
BsmAI GTCTC 1 cut(s) 798
BsmFI GGGAC 1 cut(s) 567
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 3 cut(s) 216, 909, 1241
Bsp120I GGGCCC 1 cut(s) 1239
Bsp1286I GDGCHC 2 cut(s) 515, 1243
Bsp1407I TGTACA 1 cut(s) 1116
Bsp143I GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
BspACI CCGC 5 cut(s) 148, 217, 248, 348, 1207
BspANI GGCC 3 cut(s) 216, 909, 1241
BspCNI CTCAG 3 cut(s) 286, 924, 1009
BspLI GGNNCC 8 cut(s) 163, 332, 520, 580, 670, 908, 993, 1241
BspMAI CTGCAG 1 cut(s) 461
BspPI GGATC 4 cut(s) 156, 169, 667, 755
BspT107I GGYRCC 2 cut(s) 518, 991
BsrDI GCAATG 2 cut(s) 1132, 1253
BsrGI TGTACA 1 cut(s) 1116
BsrI ACTGG 1 cut(s) 1042
BssECI CCNNGG 5 cut(s) 522, 565, 655, 875, 1043
BssMI GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
BssNAI GTATAC 1 cut(s) 1251
BssT1I CCWWGG 2 cut(s) 522, 1043
Bst1107I GTATAC 1 cut(s) 1251
Bst2UI CCWGG 4 cut(s) 166, 583, 657, 1065
Bst4CI ACNGT 7 cut(s) 186, 241, 501, 558, 809, 1085, 1096
BstAUI TGTACA 1 cut(s) 1116
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 4 cut(s) 294, 314, 911, 996
BstENI CCTNNNNNAGG 1 cut(s) 901
BstF5I GGATG 3 cut(s) 151, 885, 946
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 6 cut(s) 83, 164, 662, 712, 763, 1267
BstMAI GTCTC 1 cut(s) 798
BstMBI GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
BstMWI GCNNNNNNNGC 2 cut(s) 140, 1213
BstNI CCWGG 4 cut(s) 166, 583, 657, 1065
BstSCI CCNGG 7 cut(s) 21, 164, 565, 581, 655, 874, 1063
BstSFI CTRYAG 2 cut(s) 457, 1178
BstSLI GKGCMC 1 cut(s) 1243
BstV1I GCAGC 6 cut(s) 118, 195, 366, 799, 1138, 1191
BstV2I GAAGAC 2 cut(s) 428, 726
BstX2I RGATCY 2 cut(s) 161, 760
BstYI RGATCY 2 cut(s) 161, 760
BstZ17I GTATAC 1 cut(s) 1251
BsuRI GGCC 3 cut(s) 216, 909, 1241
BtsCI GGATG 3 cut(s) 151, 885, 946
BtsIMutI CAGTG 3 cut(s) 123, 477, 814
Cac8I GCNNGC 1 cut(s) 541
CaiI CAGNNNCTG 2 cut(s) 824, 1204
CfoI GCGC 1 cut(s) 539
Cfr13I GGNCC 6 cut(s) 107, 894, 907, 1040, 1239, 1240
Csp6I GTAC 5 cut(s) 519, 586, 601, 1035, 1117
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviAII CATG 5 cut(s) 212, 665, 930, 976, 1246
CviQI GTAC 5 cut(s) 519, 586, 601, 1035, 1117
DdeI CTNAG 4 cut(s) 294, 314, 911, 996
DpnI GATC 6 cut(s) 82, 163, 661, 711, 762, 1266
DpnII GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
DraI TTTAAA 1 cut(s) 1101
DriI GACNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 214
Eam1105I GACNNNNNGTC 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 522, 1043
Eco24I GRGCYC 2 cut(s) 515, 1243
Eco47I GGWCC 3 cut(s) 107, 894, 1040
EcoNI CCTNNNNNAGG 1 cut(s) 901
EcoO109I RGGNCCY 2 cut(s) 907, 1240
EcoRII CCWGG 4 cut(s) 164, 581, 655, 1063
EcoT14I CCWWGG 2 cut(s) 522, 1043
EcoT38I GRGCYC 2 cut(s) 515, 1243
ErhI CCWWGG 2 cut(s) 522, 1043
FaeI CATG 5 cut(s) 215, 668, 933, 979, 1249
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 567
FatI CATG 5 cut(s) 211, 664, 929, 975, 1245
FauI CCCGC 2 cut(s) 155, 241
FbaI TGATCA 1 cut(s) 1264
FblI GTMKAC 1 cut(s) 1250
Fnu4HI GCNGC 8 cut(s) 132, 209, 217, 380, 813, 1152, 1205, 1208
FokI GGATG 3 cut(s) 138, 892, 933
FriOI GRGCYC 2 cut(s) 515, 1243
Fsp4HI GCNGC 8 cut(s) 132, 209, 217, 380, 813, 1152, 1205, 1208
FspBI CTAG 3 cut(s) 698, 942, 1211
GlaI GCGC 1 cut(s) 538
GluI GCNGC 8 cut(s) 132, 209, 217, 380, 813, 1152, 1205, 1208
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 3 cut(s) 216, 909, 1241
HapII CCGG 5 cut(s) 22, 310, 534, 567, 875
HhaI GCGC 1 cut(s) 539
Hin1II CATG 5 cut(s) 215, 668, 933, 979, 1249
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 1 cut(s) 1190
HpaII CCGG 5 cut(s) 22, 310, 534, 567, 875
Hpy166II GTNNAC 3 cut(s) 894, 1117, 1251
Hpy188I TCNGA 8 cut(s) 295, 342, 441, 714, 826, 862, 1189, 1200
Hpy188III TCNNGA 6 cut(s) 159, 263, 576, 614, 747, 922
Hpy8I GTNNAC 3 cut(s) 894, 1117, 1251
HpyAV CCTTC 3 cut(s) 105, 406, 907
HpyCH4III ACNGT 7 cut(s) 186, 241, 501, 558, 809, 1085, 1096
HpyCH4IV ACGT 3 cut(s) 138, 472, 1142
HpyCH4V TGCA 5 cut(s) 211, 459, 770, 1258, 1306
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 1213
HpyF3I CTNAG 4 cut(s) 294, 314, 911, 996
HpySE526I ACGT 3 cut(s) 138, 472, 1142
Hsp92II CATG 5 cut(s) 215, 668, 933, 979, 1249
HspAI GCGC 1 cut(s) 537
KpnI GGTACC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 1264
Kzo9I GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
LmnI GCTCC 5 cut(s) 336, 398, 578, 668, 1290
Lsp1109I GCAGC 6 cut(s) 118, 195, 366, 799, 1138, 1191
LweI GCATC 3 cut(s) 118, 1138, 1191
MaeI CTAG 3 cut(s) 698, 942, 1211
MaeII ACGT 3 cut(s) 138, 472, 1142
MaeIII GTNAC 3 cut(s) 473, 870, 1271
MalI GATC 6 cut(s) 82, 163, 661, 711, 762, 1266
MboI GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
MflI RGATCY 2 cut(s) 161, 760
MhlI GDGCHC 2 cut(s) 515, 1243
MmeI TCCRAC 1 cut(s) 1104
MseI TTAA 3 cut(s) 18, 1100, 1312
MspA1I CMGCKG 1 cut(s) 1183
MspI CCGG 5 cut(s) 22, 310, 534, 567, 875
MspR9I CCNGG 7 cut(s) 23, 166, 567, 583, 657, 876, 1065
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 4 cut(s) 166, 583, 657, 1065
MwoI GCNNNNNNNGC 2 cut(s) 140, 1213
NciI CCSGG 3 cut(s) 23, 567, 876
NdeII GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
NlaIII CATG 5 cut(s) 215, 668, 933, 979, 1249
NlaIV GGNNCC 8 cut(s) 163, 332, 520, 580, 670, 908, 993, 1241
NmuCI GTSAC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 1190
PflMI CCANNNNNTGG 2 cut(s) 656, 956
PkrI GCNGC 8 cut(s) 133, 210, 218, 381, 814, 1153, 1206, 1209
Psp6I CCWGG 4 cut(s) 164, 581, 655, 1063
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 4 cut(s) 164, 581, 655, 1063
PspN4I GGNNCC 8 cut(s) 163, 332, 520, 580, 670, 908, 993, 1241
PspOMI GGGCCC 1 cut(s) 1239
PspPI GGNCC 6 cut(s) 107, 894, 907, 1040, 1239, 1240
PstI CTGCAG 1 cut(s) 461
PstNI CAGNNNCTG 2 cut(s) 824, 1204
PsuI RGATCY 2 cut(s) 161, 760
PvuII CAGCTG 1 cut(s) 1183
RsaI GTAC 5 cut(s) 520, 587, 602, 1036, 1118
RsaNI GTAC 5 cut(s) 519, 586, 601, 1035, 1117
SaqAI TTAA 3 cut(s) 18, 1100, 1312
SatI GCNGC 8 cut(s) 132, 209, 217, 380, 813, 1152, 1205, 1208
Sau3AI GATC 6 cut(s) 80, 161, 659, 709, 760, 1264
Sau96I GGNCC 6 cut(s) 107, 894, 907, 1040, 1239, 1240
ScrFI CCNGG 7 cut(s) 23, 166, 567, 583, 657, 876, 1065
SduI GDGCHC 2 cut(s) 515, 1243
SfaNI GCATC 3 cut(s) 118, 1138, 1191
SfcI CTRYAG 2 cut(s) 457, 1178
SinI GGWCC 3 cut(s) 107, 894, 1040
SmlI CTYRAG 2 cut(s) 261, 673
SmoI CTYRAG 2 cut(s) 261, 673
SsiI CCGC 5 cut(s) 148, 217, 248, 348, 1207
SspMI CTAG 3 cut(s) 698, 942, 1211
StyD4I CCNGG 7 cut(s) 21, 164, 565, 581, 655, 874, 1063
StyI CCWWGG 2 cut(s) 522, 1043
TaaI ACNGT 7 cut(s) 186, 241, 501, 558, 809, 1085, 1096
TaiI ACGT 3 cut(s) 141, 475, 1145
TaqI TCGA 1 cut(s) 1057
TatI WGTACW 1 cut(s) 1116
TauI GCSGC 2 cut(s) 219, 1210
TfiI GAWTC 1 cut(s) 1190
Tru1I TTAA 3 cut(s) 18, 1100, 1312
Tru9I TTAA 3 cut(s) 18, 1100, 1312
TscAI CASTG 3 cut(s) 130, 484, 814
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 6 cut(s) 131, 208, 379, 812, 1151, 1204
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 5 cut(s) 55, 162, 731, 896, 918
TspGWI ACGGA 3 cut(s) 144, 213, 1022
TspRI CASTG 3 cut(s) 130, 484, 814
Van91I CCANNNNNTGG 2 cut(s) 656, 956
VpaK11BI GGWCC 3 cut(s) 107, 894, 1040
XagI CCTNNNNNAGG 1 cut(s) 901
XapI RAATTY 5 cut(s) 267, 594, 608, 1108, 1300
XmiI GTMKAC 1 cut(s) 1250
XspI CTAG 3 cut(s) 698, 942, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.