RchiOBHm_Chr5g0059581

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001151
Physical Location & Seq
Reverse (-)
64588869 .. 64592805
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33618

Sequence Viewer

Length: 2445 bp
ATGAAGGGCCTCATCTTCATCTTCATCTCTCTATTCCTATTTTCAGCCTTGAATTTCTCCATTGCAGCTGATACCATATCTCCATCACAATCCATCACCGGCTCGAACACCCTGGTTTCTTCCAACCAGAGATTTGTACTAGGACTCTTCTCCCCACCCAATTCTAGAGCATGGTACTTAGGACTATGGTACAAGAAATTTCCAGAGGTTGTTGTATGGATTGCAAACAGAGAAAACCCACTTCCGGGTTCAAATGGAGCCTTGACATTATCAGAAAAAGGCACCCTCATCCTTTCAGACCAAATGAACAAGACAATTTGGTTCTCAAATTCTTCCAGAGTAGCCGAAAACCCAATTGCGCAGCTTTTGGTGACGGGAAATCTTGTTGTCAGAGACCAGGCGTCTCCAACAGCAGCAGCAGAGTCCGAAAGCTATGTTTTTCAAAGCTTTGATTTCCCATCAAACACGCTTTTACCAGATATGAAGGTGGGGTGGGACTTCAGAACTGGCCAAAACCATTTCTTGACTAGTTGGAAAAATGCTAGCGATCCCTCCCCTGGAGAATATACCTATAGGATTGAAAACCTTGAGTTGCCTCAGCTTGTTGTCGCTCAAGGATCAAAGAAAGTGTTCAGGACAGGGCCCTGGAATGGTCTTCGATTTACAGGTACTCCTGTTTCGGTTGTTACAGCAGCATCATTTTTGGTACCAATCTTTGTCTACAATACCAAGGAGTTGTACTACTCATATGAGGTTGCTGATGATTCTATTCTGACAAGAGTGACGCTGACTGAGTCGGGTTTGGCTCAGCGGCTAGTGATGAATGAAGGGAGCAGTGAATGGGCTGTTATGTACACTCTGCAGAACAATCAGTGTGATGATTATGGTCAGTGTGGGCCAAATGGCATCTGCAAGATTTACAAATCACCCATTTGTGAATGCTTGCAGGGGTTTGTTCCCAAATCTCCAAAGGAATGGGAGTTTCTTAACTGGACAACCGGGTGCACGAGGAGGACAGCACTGCATTGTGAGAGAGGGGAAGGATTTGTGAAAGTTAATAATGTGAAACTGCCGGACTTGTTGGATTTTCTGGTCAACAAAACCATGCTCCGCGGAGAGGAATGTGAGGCAGAGTGTTTGAGGAACTGCTCCTGTATGGCTTATGCCAATTCGAACATAACCAGTGGAGGCAAAGGATGTCTTATGTGGTTTGGCAAGCTGATTGATATGCGGGAGATCGTTGAAGATAGTGACCAGATATATATTCGGATGCCAGCTTCCGAACTAGGTGATACCAGCCAGAAACATAAAAGAGTATTGCTCGTCTCGGTCATATCATCAGCAGGTTTTGTGCTTCTTTTTCTGGGTTTCTCATGCTGCTATATTTATGTGAAGAAGAGGGAAAAGAAAATGGGCTCAAGAACTAGTGAGGACTTGGAGTTGCCACTGTTTGATTATGGTAGAATTGCAACCGCCACCAATGAATTCTCCGGGACAAACAAACTGGGAGAGGGAGGTTTTGGTCCAGTTTACAAGGCCAAATTATCACAAGAAGAGTTTGTAGCAGTAAAGAGACTCTCAAAAGATTCTGGGCAAGGTCTTGAAGAGTTTAAGAACGAAGTTACAATGATGGCTAATCTTCAACACTGGAATCTTGTTAAACTTCTGGGCTGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCCAACAAAAGCTTGGACTGCTTTATTTTCGATGAAAACAACAAGTTGTTGCTGAAGTGGCAAAAGCGATTTGACATAATAGTAGGAATTGCTAGAGGACTTCTCTACCTACACCAAGACTCCAGGCTAAGAATCATTCATAGGGACCTTAAGAGTAGCAACATCTTACTCGATGATGAGCTGAACCCCAAAATTTCTGACTTTGGTATAGCAAGAATTTTCGGACGCAATCAGAATGAAGCTAAAACAAAACGAGTAATTGGCACATACGGATATATGTCCCCGGAGTATGCAATTGATGGAAAATTTTCAGTGAAATCTGATGTCTTCAGCTTTGGAGTGCTTTTGTTAGAGATAGTAAGCGGCAGAAAGAACAGAGGGTTTCATCATCCTGATCACCATCACAATCTTTTGGGACATGCATGGTTATTGTGGAAACAAAACAAGGGTTTGGAGCTAATGGATGTATGCCTGGAGGACTCATATGTTGAGTTTGAATTGCTAAGGTGCATTCAAGTGGGTCTGTTGTGTGTTCAAAAGCTGCCTGAAGACAGACCAGTAATGTCATCGGTGGTTCTTATGTTAAGCAATGAGGGAGCAACTTTACCTCAGCCAAAGGAGCCTGGATTCTTTATAGAAAGAGGTTCCTTGGACTTTGATAGCTTAAGAGACCCAGGAAAAAGCAACACAGGAAACACAATTACCATTACCACAGTGCAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

814

Amino Acids

91.63

Weight (kDa)

6.51

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 179 6.1e-32 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 9.4e-28 S-locus glycoprotein domain
PAN_2 PF08276 343 - 410 6.1e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 499 - 697 5.1e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 499 - 696 2.1e-44 Protein kinase domain
DUF3403 PF11883 769 - 814 2.6e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 1334
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 281, 706
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 1113
AciI CCGC 6 cut(s) 811, 1111, 1113, 1231, 1473, 2079
AclWI GGATC 2 cut(s) 542, 625
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 7 cut(s) 52, 197, 328, 1484, 1908, 1932, 2021
AcuI CTGAAG 4 cut(s) 484, 1790, 2029, 2283
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 8 cut(s) 138, 176, 191, 670, 708, 740, 854, 1712
AfiI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
AflII CTTAAG 2 cut(s) 1865, 2380
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 2 cut(s) 527, 1424
AjnI CCWGG 8 cut(s) 111, 396, 556, 644, 1838, 2187, 2338, 2389
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 5 cut(s) 387, 408, 1330, 1567, 2379
Alw44I GTGCAC 1 cut(s) 1003
AlwI GGATC 2 cut(s) 542, 625
AoxI GGCC 5 cut(s) 7, 508, 641, 896, 1536
ApaI GGGCCC 1 cut(s) 645
ApaLI GTGCAC 1 cut(s) 1003
ApeKI GCWGC 9 cut(s) 65, 361, 413, 416, 692, 1377, 1671, 1674, 2257
ApoI RAATTY 7 cut(s) 52, 197, 328, 1484, 1908, 1932, 2021
Asp700I GAANNNNTTC 2 cut(s) 629, 2023
Asp718I GGTACC 1 cut(s) 706
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 6 cut(s) 7, 641, 642, 896, 1523, 1861
AsuC2I CCSGG 4 cut(s) 246, 1000, 1492, 2000
AsuHPI GGTGA 5 cut(s) 88, 382, 918, 1301, 2105
AsuII TTCGAA 1 cut(s) 1172
AsuNHI GCTAGC 1 cut(s) 542
AvaII GGWCC 2 cut(s) 1523, 1861
BaeGI GKGCMC 2 cut(s) 645, 1007
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 2 cut(s) 281, 706
BanII GRGCYC 2 cut(s) 645, 1418
BauI CACGAG 1 cut(s) 1006
BbsI GAAGAC 3 cut(s) 647, 2035, 2271
Bbv12I GWGCWC 1 cut(s) 1007
BbvCI CCTCAGC 2 cut(s) 597, 2325
BbvI GCAGC 9 cut(s) 77, 373, 425, 428, 704, 1364, 1658, 1661, 2244
BccI CCATC 6 cut(s) 91, 101, 466, 1624, 2009, 2124
BciT130I CCWGG 8 cut(s) 113, 398, 558, 646, 1840, 2189, 2340, 2391
BclI TGATCA 1 cut(s) 2110
BcnI CCSGG 4 cut(s) 246, 1000, 1492, 2000
BcoDI GTCTC 5 cut(s) 387, 408, 1330, 1567, 2379
BcuI ACTAGT 2 cut(s) 527, 1424
BfaI CTAG 9 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 1809, 2439
BfmI CTRYAG 2 cut(s) 571, 860
BfrI CTTAAG 2 cut(s) 1865, 2380
BfuAI ACCTGC 1 cut(s) 1334
BlpI GCTNAGC 1 cut(s) 807
Bme18I GGWCC 2 cut(s) 1523, 1861
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 6 cut(s) 7, 641, 642, 896, 1523, 1861
BmiI GGNNCC 7 cut(s) 259, 283, 643, 708, 1862, 2337, 2362
BmrI ACTGGG 1 cut(s) 1514
BmsI GCATC 4 cut(s) 704, 915, 1260, 1686
BmtI GCTAGC 1 cut(s) 546
BmuI ACTGGG 1 cut(s) 1514
BpiI GAAGAC 3 cut(s) 647, 2035, 2271
BplI GAGNNNNNCTC 4 cut(s) 1305, 1337, 1803, 1835
BpmI CTGGAG 3 cut(s) 579, 1822, 2210
Bpu10I CCTNAGC 3 cut(s) 597, 2219, 2325
Bpu1102I GCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 1172
BpuEI CTTGAG 3 cut(s) 597, 608, 1402
BpuMI CCSGG 4 cut(s) 246, 1000, 1492, 2000
BsaBI GATNNNNATC 2 cut(s) 1700, 2115
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 2 cut(s) 387, 2379
BsaJI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1998, 2364, 2389
BsaXI ACNNNNNCTCC 6 cut(s) 64, 94, 655, 685, 1820, 1850
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 7 cut(s) 511, 995, 1182, 1509, 1526, 1652, 2273
Bse3DI GCAATG 2 cut(s) 60, 2311
Bse8I GATNNNNATC 2 cut(s) 1700, 2115
BseBI CCWGG 8 cut(s) 113, 398, 558, 646, 1840, 2189, 2340, 2391
BseDI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1998, 2364, 2389
BseGI GGATG 6 cut(s) 288, 1202, 1275, 1701, 2104, 2185
BseJI GATNNNNATC 2 cut(s) 1700, 2115
BseLI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
BseMI GCAATG 2 cut(s) 60, 2311
BseMII CTCAG 4 cut(s) 611, 783, 821, 2339
BseNI ACTGG 7 cut(s) 511, 995, 1182, 1509, 1526, 1652, 2273
BseRI GAGGAG 1 cut(s) 1024
BseSI GKGCMC 2 cut(s) 645, 1007
BseXI GCAGC 9 cut(s) 77, 373, 425, 428, 704, 1364, 1658, 1661, 2244
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 5 cut(s) 9, 510, 643, 898, 1538
BshNI GGYRCC 2 cut(s) 281, 706
BsiHKAI GWGCWC 1 cut(s) 1007
BsiSI CCGG 6 cut(s) 99, 245, 999, 1073, 1491, 2000
BslFI GGGAC 5 cut(s) 509, 1507, 1874, 1981, 2145
BslI CCNNNNNNNGG 5 cut(s) 244, 245, 557, 650, 1117
BsmAI GTCTC 5 cut(s) 387, 408, 1330, 1567, 2379
BsmBI CGTCTC 2 cut(s) 408, 1330
BsmFI GGGAC 5 cut(s) 509, 1507, 1874, 1981, 2145
BsmI GAATGC 2 cut(s) 944, 2226
BsnI GGCC 5 cut(s) 9, 510, 643, 898, 1538
Bso31I GGTCTC 2 cut(s) 387, 2379
Bsp119I TTCGAA 1 cut(s) 1172
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 645, 1007, 1418
Bsp1407I TGTACA 1 cut(s) 852
Bsp143I GATC 4 cut(s) 547, 617, 1236, 2110
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 6 cut(s) 811, 1111, 1113, 1231, 1473, 2079
BspANI GGCC 5 cut(s) 9, 510, 643, 898, 1538
BspCNI CTCAG 4 cut(s) 610, 784, 820, 2338
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 7 cut(s) 259, 283, 643, 708, 1862, 2337, 2362
BspMAI CTGCAG 1 cut(s) 864
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 542, 625
BspT104I TTCGAA 1 cut(s) 1172
BspT107I GGYRCC 2 cut(s) 281, 706
BspTI CTTAAG 2 cut(s) 1865, 2380
BspTNI GGTCTC 2 cut(s) 387, 2379
BsrDI GCAATG 2 cut(s) 60, 2311
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 852
BsrI ACTGG 7 cut(s) 511, 995, 1182, 1509, 1526, 1652, 2273
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1998, 2364, 2389
BssMI GATC 4 cut(s) 547, 617, 1236, 2110
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 1006
BssT1I CCWWGG 2 cut(s) 729, 2364
Bst2BI CACGAG 1 cut(s) 1006
Bst2UI CCWGG 8 cut(s) 113, 398, 558, 646, 1840, 2189, 2340, 2391
Bst4CI ACNGT 2 cut(s) 1449, 2431
Bst6I CTCTTC 4 cut(s) 152, 1391, 1548, 1599
BstACI GRCGYC 1 cut(s) 401
BstAFI CTTAAG 2 cut(s) 1865, 2380
BstAUI TGTACA 1 cut(s) 852
BstBI TTCGAA 1 cut(s) 1172
BstC8I GCNNGC 5 cut(s) 544, 944, 1217, 1275, 2436
BstDEI CTNAG 7 cut(s) 178, 597, 792, 807, 1844, 2219, 2325
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 6 cut(s) 288, 1202, 1275, 1701, 2104, 2185
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 4 cut(s) 550, 620, 1239, 2113
BstMAI GTCTC 5 cut(s) 387, 408, 1330, 1567, 2379
BstMBI GATC 4 cut(s) 547, 617, 1236, 2110
BstMWI GCNNNNNNNGC 3 cut(s) 1734, 1774, 2335
BstNI CCWGG 8 cut(s) 113, 398, 558, 646, 1840, 2189, 2340, 2391
BstNSI RCATGY 2 cut(s) 1717, 2138
BstSFI CTRYAG 2 cut(s) 571, 860
BstSLI GKGCMC 2 cut(s) 645, 1007
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 9 cut(s) 77, 373, 425, 428, 704, 1364, 1658, 1661, 2244
BstV2I GAAGAC 3 cut(s) 647, 2035, 2271
BstXI CCANNNNNNTGG 1 cut(s) 975
BsuRI GGCC 5 cut(s) 9, 510, 643, 898, 1538
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 6 cut(s) 288, 1202, 1275, 1701, 2104, 2185
BtsI GCAGTG 2 cut(s) 841, 1019
BtsIMutI CAGTG 9 cut(s) 841, 878, 896, 1019, 1189, 1445, 1645, 2034, 2436
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 5 cut(s) 544, 944, 1217, 1275, 2436
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 6 cut(s) 7, 641, 642, 896, 1523, 1861
Cfr42I CCGCGG 1 cut(s) 1114
CseI GACGC 3 cut(s) 390, 793, 1950
Csp6I GTAC 8 cut(s) 137, 175, 190, 669, 707, 739, 853, 1711
CviAII CATG 6 cut(s) 171, 1105, 1374, 1714, 2135, 2139
CviQI GTAC 8 cut(s) 137, 175, 190, 669, 707, 739, 853, 1711
DdeI CTNAG 7 cut(s) 178, 597, 792, 807, 1844, 2219, 2325
DpnI GATC 4 cut(s) 549, 619, 1238, 2112
DpnII GATC 4 cut(s) 547, 617, 1236, 2110
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 4 cut(s) 152, 1391, 1548, 1599
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 4 cut(s) 152, 1391, 1548, 1599
Eco130I CCWWGG 2 cut(s) 729, 2364
Eco24I GRGCYC 2 cut(s) 645, 1418
Eco31I GGTCTC 2 cut(s) 387, 2379
Eco47I GGWCC 2 cut(s) 1523, 1861
Eco57I CTGAAG 4 cut(s) 484, 1790, 2029, 2283
EcoO109I RGGNCCY 4 cut(s) 7, 641, 642, 1861
EcoRI GAATTC 1 cut(s) 1484
EcoRII CCWGG 8 cut(s) 111, 396, 556, 644, 1838, 2187, 2338, 2389
EcoT14I CCWWGG 2 cut(s) 729, 2364
EcoT22I ATGCAT 1 cut(s) 2140
EcoT38I GRGCYC 2 cut(s) 645, 1418
ErhI CCWWGG 2 cut(s) 729, 2364
Esp3I CGTCTC 2 cut(s) 408, 1330
FaeI CATG 6 cut(s) 174, 1108, 1377, 1717, 2138, 2142
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 5 cut(s) 509, 1507, 1874, 1981, 2145
FatI CATG 6 cut(s) 170, 1104, 1373, 1713, 2134, 2138
FauI CCCGC 1 cut(s) 1224
FauNDI CATATG 2 cut(s) 748, 2200
FbaI TGATCA 1 cut(s) 2110
FblI GTMKAC 1 cut(s) 720
FokI GGATG 6 cut(s) 275, 1209, 1282, 1708, 2091, 2192
FriOI GRGCYC 2 cut(s) 645, 1418
FspBI CTAG 9 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 1809, 2439
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GsuI CTGGAG 3 cut(s) 579, 1822, 2210
HaeIII GGCC 5 cut(s) 9, 510, 643, 898, 1538
HapII CCGG 6 cut(s) 99, 245, 999, 1073, 1491, 2000
HgaI GACGC 3 cut(s) 390, 793, 1950
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 6 cut(s) 174, 1108, 1377, 1717, 2138, 2142
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 2 cut(s) 445, 1726
HpaII CCGG 6 cut(s) 99, 245, 999, 1073, 1491, 2000
HphI GGTGA 5 cut(s) 88, 382, 918, 1301, 2105
Hpy166II GTNNAC 5 cut(s) 721, 855, 1005, 1096, 1531
Hpy188III TCNNGA 8 cut(s) 165, 203, 336, 523, 634, 1419, 1601, 2108
Hpy8I GTNNAC 5 cut(s) 721, 855, 1005, 1096, 1531
HpyAV CCTTC 4 cut(s) 478, 821, 1034, 1676
HpyCH4III ACNGT 2 cut(s) 1449, 2431
HpyF10VI GCNNNNNNNGC 3 cut(s) 1734, 1774, 2335
HpyF3I CTNAG 7 cut(s) 178, 597, 792, 807, 1844, 2219, 2325
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 6 cut(s) 174, 1108, 1377, 1717, 2138, 2142
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 710
Ksp22I TGATCA 1 cut(s) 2110
KspI CCGCGG 1 cut(s) 1114
Kzo9I GATC 4 cut(s) 547, 617, 1236, 2110
LmnI GCTCC 7 cut(s) 257, 831, 1113, 1154, 2170, 2312, 2335
Lsp1109I GCAGC 9 cut(s) 77, 373, 425, 428, 704, 1364, 1658, 1661, 2244
LweI GCATC 4 cut(s) 704, 915, 1260, 1686
MaeI CTAG 9 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 1809, 2439
MaeIII GTNAC 5 cut(s) 370, 685, 781, 1250, 1621
MalI GATC 4 cut(s) 549, 619, 1238, 2112
MboI GATC 4 cut(s) 547, 617, 1236, 2110
MfeI CAATTG 2 cut(s) 354, 2010
MhlI GDGCHC 3 cut(s) 645, 1007, 1418
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 6 cut(s) 138, 431, 803, 1569, 1829, 2189
MmeI TCCRAC 4 cut(s) 147, 431, 512, 1062
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 2140
MroXI GAANNNNTTC 2 cut(s) 629, 2023
MscI TGGCCA 1 cut(s) 510
MseI TTAA 7 cut(s) 987, 1056, 1611, 1659, 1866, 2300, 2381
MslI CAYNNNNRTG 1 cut(s) 2231
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 68, 811, 1113
MspCI CTTAAG 2 cut(s) 1865, 2380
MspI CCGG 6 cut(s) 99, 245, 999, 1073, 1491, 2000
MunI CAATTG 2 cut(s) 354, 2010
Mva1269I GAATGC 2 cut(s) 944, 2226
MvaI CCWGG 8 cut(s) 113, 398, 558, 646, 1840, 2189, 2340, 2391
MvnI CGCG 1 cut(s) 1113
MwoI GCNNNNNNNGC 3 cut(s) 1734, 1774, 2335
NciI CCSGG 4 cut(s) 246, 1000, 1492, 2000
NdeI CATATG 2 cut(s) 748, 2200
NdeII GATC 4 cut(s) 547, 617, 1236, 2110
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 6 cut(s) 174, 1108, 1377, 1717, 2138, 2142
NlaIV GGNNCC 7 cut(s) 259, 283, 643, 708, 1862, 2337, 2362
NmuCI GTSAC 3 cut(s) 370, 781, 1250
NsbI TGCGCA 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 2140
NspI RCATGY 2 cut(s) 1717, 2138
NspV TTCGAA 1 cut(s) 1172
PctI GAATGC 2 cut(s) 944, 2226
PdmI GAANNNNTTC 2 cut(s) 629, 2023
PfeI GAWTC 5 cut(s) 764, 1586, 1651, 1848, 2343
PflFI GACNNNGTC 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 1490
PleI GAGTC 6 cut(s) 138, 430, 802, 1569, 1829, 2189
PpsI GAGTC 6 cut(s) 138, 430, 802, 1569, 1829, 2189
PpuMI RGGWCCY 1 cut(s) 1861
Psp5II RGGWCCY 1 cut(s) 1861
Psp6I CCWGG 8 cut(s) 111, 396, 556, 644, 1838, 2187, 2338, 2389
PspGI CCWGG 8 cut(s) 111, 396, 556, 644, 1838, 2187, 2338, 2389
PspN4I GGNNCC 7 cut(s) 259, 283, 643, 708, 1862, 2337, 2362
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 6 cut(s) 7, 641, 642, 896, 1523, 1861
PspPPI RGGWCCY 1 cut(s) 1861
PstI CTGCAG 1 cut(s) 864
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 8 cut(s) 138, 176, 191, 670, 708, 740, 854, 1712
RsaNI GTAC 8 cut(s) 137, 175, 190, 669, 707, 739, 853, 1711
RseI CAYNNNNRTG 1 cut(s) 2231
SacII CCGCGG 1 cut(s) 1114
SaqAI TTAA 7 cut(s) 987, 1056, 1611, 1659, 1866, 2300, 2381
Sau3AI GATC 4 cut(s) 547, 617, 1236, 2110
Sau96I GGNCC 6 cut(s) 7, 641, 642, 896, 1523, 1861
SchI GAGTC 6 cut(s) 138, 431, 803, 1569, 1829, 2189
SduI GDGCHC 3 cut(s) 645, 1007, 1418
SfaNI GCATC 4 cut(s) 704, 915, 1260, 1686
SfcI CTRYAG 2 cut(s) 571, 860
Sfr303I CCGCGG 1 cut(s) 1114
SfuI TTCGAA 1 cut(s) 1172
SgrBI CCGCGG 1 cut(s) 1114
SinI GGWCC 2 cut(s) 1523, 1861
SmiMI CAYNNNNRTG 1 cut(s) 2231
SmlI CTYRAG 5 cut(s) 587, 612, 1417, 1865, 2380
SmoI CTYRAG 5 cut(s) 587, 612, 1417, 1865, 2380
SpeI ACTAGT 2 cut(s) 527, 1424
SsiI CCGC 6 cut(s) 811, 1111, 1113, 1231, 1473, 2079
SspMI CTAG 9 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 1809, 2439
StyI CCWWGG 2 cut(s) 729, 2364
TaaI ACNGT 2 cut(s) 1449, 2431
TaqI TCGA 5 cut(s) 104, 658, 1172, 1746, 1887
TaqII GACCGA 1 cut(s) 1318
TatI WGTACW 4 cut(s) 136, 738, 852, 1710
TauI GCSGC 2 cut(s) 814, 2082
TfiI GAWTC 5 cut(s) 764, 1586, 1651, 1848, 2343
Tru1I TTAA 7 cut(s) 987, 1056, 1611, 1659, 1866, 2300, 2381
Tru9I TTAA 7 cut(s) 987, 1056, 1611, 1659, 1866, 2300, 2381
TscAI CASTG 9 cut(s) 841, 878, 896, 1026, 1189, 1452, 1652, 2034, 2436
TseFI GTSAC 3 cut(s) 370, 781, 1250
TseI GCWGC 9 cut(s) 65, 361, 413, 416, 692, 1377, 1671, 1674, 2257
Tsp45I GTSAC 3 cut(s) 370, 781, 1250
TspGWI ACGGA 1 cut(s) 2001
TspRI CASTG 9 cut(s) 841, 878, 896, 1026, 1189, 1452, 1652, 2034, 2436
Tth111I GACNNNGTC 1 cut(s) 793
Vha464I CTTAAG 2 cut(s) 1865, 2380
VneI GTGCAC 1 cut(s) 1003
VpaK11BI GGWCC 2 cut(s) 1523, 1861
XapI RAATTY 7 cut(s) 52, 197, 328, 1484, 1908, 1932, 2021
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 2 cut(s) 1717, 2138
XcmI CCANNNNNNNNNTGG 1 cut(s) 1726
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 629, 2023
XspI CTAG 9 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 1809, 2439
Zsp2I ATGCAT 1 cut(s) 2140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.