RchiOBHm_Chr5g0036351

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001151
Physical Location & Seq
Forward (+)
30545179 .. 30546162
984 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31517

Sequence Viewer

Length: 984 bp
ATGAACAACACCATTTGGTCTTCCAATTCTTCCAGAGTCCCAGAAAATCCACTTGCACAGCTTTTGGAGACTGGAAATTTTGTTGTTAGAGACAGTGCTATGACAGGTTCTGAAAGCTATGTGTGGCAAAGTTTTGATTTCCCATCAGACACAATATTGTTAGCGGGGATGAAGCTGGGATGGAACTTCAAAAGGGGCCTCAACCAATATATGACTTCATGGAAAAATGCCACTGATCCATCTCTTGGGGCATATACCTATGGGATGGATAATGATGTGTTGCCTCAGTTTGTTTTTGCAGAAGGGTCTAGCAAAAGGTTCCATACTGGACCTTGGAATGGTCTTCGATTCCCTGGCTCTTATGTTTCGAACAATACTAACACAGTTATAAAGCCAACTTTTGTGTACAATACCAATGAGATATATTACACGTTTGAGGCTGCTGAAAGCTCTATTACTACAAGACTACAGTTGTCTGAATTGGGTGTGATGCAGCGACTAGTGCTGAATAAGGGAAGCACAGAATGGGCCCTTATGTATATTTTTCTGGATGATCAGTGTAAAAATTATGGGGAGTATGGAGCAAATGGCATTTGCAGAATTAACAAGGTACCAATTTGCGATTGCTTGCAGGGGTTTGTTCCTAAATCACCGAAAAAGTGGGTGGTGCTTAATAGGACAGGTGGTTGCAAAAGGGAGACGACACTAGATTGCCAAAAGGGAGAAGGGTTTCTAAAACTGCAAAATGTGAAACCGCCAGACCTGCTGGATATTTTGGTGAACAAGAGCATGAGCACGGAGGAATGTGAGGCAAATTGCTTGAAGAATTGTTCCTGCATAGCTTATTCGAATTCAGATATTCGGAGTGGAGGCAGTGGTTGTGTTATGTGGTTTGGAAATCTGAGTGATATGCGGGAGTTTGTTGAGGATCTTGGTAAGCAAGATATCTTTATTCAGATGCCAGCTTCTGAACTAGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

327

Amino Acids

36.64

Weight (kDa)

5.45

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 2 - 75 8.6e-20 D-mannose binding lectin
S_locus_glycop PF00954 107 - 217 1.2e-22 S-locus glycoprotein domain
PAN_2 PF08276 238 - 304 8.3e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 771
Acc65I GGTACC 1 cut(s) 610
AccB1I GGYRCC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 3 cut(s) 164, 755, 913
AclWI GGATC 2 cut(s) 230, 936
AcsI RAATTY 2 cut(s) 76, 850
AfaI GTAC 2 cut(s) 407, 612
AfiI CCNNNNNNNGG 2 cut(s) 245, 338
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 2 cut(s) 190, 823
AhlI ACTAGT 1 cut(s) 499
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 6 cut(s) 61, 117, 175, 450, 842, 965
AluI AGCT 6 cut(s) 61, 117, 175, 450, 842, 965
Alw21I GWGCWC 1 cut(s) 797
Alw26I GTCTC 3 cut(s) 62, 84, 692
AlwI GGATC 2 cut(s) 230, 936
AlwNI CAGNNNCTG 2 cut(s) 110, 968
AoxI GGCC 2 cut(s) 196, 528
ApaI GGGCCC 1 cut(s) 532
ApeKI GCWGC 2 cut(s) 440, 493
ApoI RAATTY 2 cut(s) 76, 850
Asp700I GAANNNNTTC 1 cut(s) 729
Asp718I GGTACC 1 cut(s) 610
AspS9I GGNCC 4 cut(s) 196, 329, 528, 529
AsuHPI GGTGA 2 cut(s) 642, 790
AsuII TTCGAA 2 cut(s) 368, 848
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 610
BanII GRGCYC 1 cut(s) 532
BbsI GAAGAC 2 cut(s) 12, 335
Bbv12I GWGCWC 1 cut(s) 797
BbvI GCAGC 2 cut(s) 427, 505
BccI CCATC 4 cut(s) 151, 174, 247, 259
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 3 cut(s) 62, 84, 692
BcuI ACTAGT 1 cut(s) 499
BfaI CTAG 4 cut(s) 309, 500, 707, 974
BfmI CTRYAG 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 771
BisI GCNGC 2 cut(s) 441, 494
BlsI GCNGC 2 cut(s) 442, 495
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 4 cut(s) 196, 329, 528, 529
BmiI GGNNCC 4 cut(s) 197, 320, 530, 612
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 480, 948
BpiI GAAGAC 2 cut(s) 12, 335
Bpu14I TTCGAA 2 cut(s) 368, 848
BsaJI CCNNGG 2 cut(s) 332, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 338
Bse1I ACTGG 2 cut(s) 76, 331
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 332, 352
BseGI GGATG 4 cut(s) 174, 185, 270, 556
BseLI CCNNNNNNNGG 2 cut(s) 245, 338
BseMII CTCAG 2 cut(s) 299, 893
BseNI ACTGG 2 cut(s) 76, 331
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 427, 505
BseYI CCCAGC 1 cut(s) 175
BshFI GGCC 2 cut(s) 198, 530
BshNI GGYRCC 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 797
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 245, 338
BsmAI GTCTC 3 cut(s) 62, 84, 692
BsmBI CGTCTC 1 cut(s) 692
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 2 cut(s) 198, 530
Bsp119I TTCGAA 2 cut(s) 368, 848
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 2 cut(s) 532, 797
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 3 cut(s) 235, 553, 928
BspACI CCGC 3 cut(s) 164, 755, 913
BspANI GGCC 2 cut(s) 198, 530
BspCNI CTCAG 2 cut(s) 298, 894
BspLI GGNNCC 4 cut(s) 197, 320, 530, 612
BspMI ACCTGC 1 cut(s) 771
BspPI GGATC 2 cut(s) 230, 936
BspT104I TTCGAA 2 cut(s) 368, 848
BspT107I GGYRCC 1 cut(s) 610
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 76, 331
BssECI CCNNGG 2 cut(s) 332, 352
BssMI GATC 3 cut(s) 235, 553, 928
BssT1I CCWWGG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 3 cut(s) 95, 385, 471
BstAUI TGTACA 1 cut(s) 405
BstBI TTCGAA 2 cut(s) 368, 848
BstC8I GCNNGC 2 cut(s) 629, 963
BstDEI CTNAG 2 cut(s) 285, 902
BstF5I GGATG 4 cut(s) 174, 185, 270, 556
BstKTI GATC 3 cut(s) 238, 556, 931
BstMAI GTCTC 3 cut(s) 62, 84, 692
BstMBI GATC 3 cut(s) 235, 553, 928
BstMWI GCNNNNNNNGC 2 cut(s) 502, 763
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 467
BstSLI GKGCMC 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 427, 505
BstV2I GAAGAC 2 cut(s) 12, 335
BstX2I RGATCY 1 cut(s) 928
BstYI RGATCY 1 cut(s) 928
BsuRI GGCC 2 cut(s) 198, 530
BtsCI GGATG 4 cut(s) 174, 185, 270, 556
BtsI GCAGTG 1 cut(s) 880
BtsIMutI CAGTG 4 cut(s) 100, 231, 563, 880
BveI ACCTGC 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 629, 963
CaiI CAGNNNCTG 2 cut(s) 110, 968
Cfr13I GGNCC 4 cut(s) 196, 329, 528, 529
Csp6I GTAC 2 cut(s) 406, 611
CviAII CATG 2 cut(s) 219, 790
CviQI GTAC 2 cut(s) 406, 611
DdeI CTNAG 2 cut(s) 285, 902
DpnI GATC 3 cut(s) 237, 555, 930
DpnII GATC 3 cut(s) 235, 553, 928
Eco130I CCWWGG 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 532
Eco32I GATATC 1 cut(s) 946
Eco47I GGWCC 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 196, 529
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 352
EcoRV GATATC 1 cut(s) 946
EcoT14I CCWWGG 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 332
Esp3I CGTCTC 1 cut(s) 692
FaeI CATG 2 cut(s) 222, 793
FaqI GGGAC 1 cut(s) 23
FatI CATG 2 cut(s) 218, 789
FauI CCCGC 2 cut(s) 157, 906
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 441, 494
FokI GGATG 4 cut(s) 181, 192, 277, 563
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 2 cut(s) 441, 494
FspBI CTAG 4 cut(s) 309, 500, 707, 974
GluI GCNGC 2 cut(s) 441, 494
GsaI CCCAGC 1 cut(s) 179
HaeIII GGCC 2 cut(s) 198, 530
Hin1II CATG 2 cut(s) 222, 793
HinfI GANTC 2 cut(s) 36, 348
HphI GGTGA 2 cut(s) 642, 790
Hpy166II GTNNAC 2 cut(s) 406, 781
Hpy188I TCNGA 8 cut(s) 112, 148, 478, 856, 864, 903, 957, 970
Hpy188III TCNNGA 2 cut(s) 33, 548
Hpy8I GTNNAC 2 cut(s) 406, 781
HpyAV CCTTC 2 cut(s) 296, 719
HpyCH4III ACNGT 3 cut(s) 95, 385, 471
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 8 cut(s) 56, 299, 493, 597, 631, 690, 742, 837
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 763
HpyF3I CTNAG 2 cut(s) 285, 902
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 2 cut(s) 222, 793
KpnI GGTACC 1 cut(s) 614
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 3 cut(s) 235, 553, 928
LmnI GCTCC 1 cut(s) 581
Lsp1109I GCAGC 2 cut(s) 427, 505
LweI GCATC 2 cut(s) 480, 948
MaeI CTAG 4 cut(s) 309, 500, 707, 974
MaeII ACGT 1 cut(s) 431
MalI GATC 3 cut(s) 237, 555, 930
MboI GATC 3 cut(s) 235, 553, 928
MboII GAAGA 4 cut(s) 12, 21, 335, 835
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 2 cut(s) 532, 797
MluCI AATT 9 cut(s) 25, 76, 479, 565, 600, 615, 814, 826, 850
MlyI GAGTC 1 cut(s) 45
MnlI CCTC 7 cut(s) 209, 294, 430, 793, 802, 863, 919
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 2 cut(s) 603, 672
MspR9I CCNGG 1 cut(s) 354
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 502, 763
NdeII GATC 3 cut(s) 235, 553, 928
NlaIII CATG 2 cut(s) 222, 793
NlaIV GGNNCC 4 cut(s) 197, 320, 530, 612
NspV TTCGAA 2 cut(s) 368, 848
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 1 cut(s) 348
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 2 cut(s) 442, 495
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PsiI TTATAA 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 175
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 4 cut(s) 197, 320, 530, 612
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 4 cut(s) 196, 329, 528, 529
PstNI CAGNNNCTG 2 cut(s) 110, 968
PsuI RGATCY 1 cut(s) 928
RsaI GTAC 2 cut(s) 407, 612
RsaNI GTAC 2 cut(s) 406, 611
SaqAI TTAA 2 cut(s) 603, 672
SatI GCNGC 2 cut(s) 441, 494
Sau3AI GATC 3 cut(s) 235, 553, 928
Sau96I GGNCC 4 cut(s) 196, 329, 528, 529
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 532, 797
SfaNI GCATC 2 cut(s) 480, 948
SfcI CTRYAG 1 cut(s) 467
SfuI TTCGAA 2 cut(s) 368, 848
SinI GGWCC 1 cut(s) 329
SpeI ACTAGT 1 cut(s) 499
Sse9I AATT 9 cut(s) 25, 76, 479, 565, 600, 615, 814, 826, 850
SsiI CCGC 3 cut(s) 164, 755, 913
SspI AATATT 1 cut(s) 156
SspMI CTAG 4 cut(s) 309, 500, 707, 974
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 332
TaaI ACNGT 3 cut(s) 95, 385, 471
TaiI ACGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 346, 368, 848
TasI AATT 9 cut(s) 25, 76, 479, 565, 600, 615, 814, 826, 850
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 2 cut(s) 603, 672
Tru9I TTAA 2 cut(s) 603, 672
TscAI CASTG 4 cut(s) 100, 238, 563, 880
TseI GCWGC 2 cut(s) 440, 493
TspDTI ATGAA 3 cut(s) 17, 185, 207
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 4 cut(s) 100, 238, 563, 880
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 2 cut(s) 76, 850
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 309, 500, 707, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.