Rh5BG249300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001151
Physical Location & Seq
Forward (+)
30553084 .. 30557547
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG249300.1

Sequence Viewer

Length: 1593 bp
ATGAAGGGCCTTCCTTTCATCTTATTCATCTCTCTTTTCCATGTTTTAGTGTTCAGTTTATGCAGTGCAGTTGATACTATATCTCCAACACAATCCATCACCCGCTCCAATACCTTGGTTTCTTCCGGTGGAAGATTTGAATTAGGACTCTTCCCCTCCGGAAACTCTAAAGCATGGTATTTAGGAATTCGGTACACCAAGTTTTCGAATATAACCGTATGGGTCGCCAACAGAGAAAACCCAATTCCAGGTTCAGATGGAGCCCTGACATTAACCGAAAATGGTACTTTGGTCCTTTTAGACCAGATGAACAACACTGTATGGTATTCTAATTTTTCCACAGTAGTTGAAGATCCTGTTGCACAGCTTTTGGAGACTGGAAATTTTGTTGTCAGAGACAAGGCTACAGCGGGTTCGGAAAGTTATGTATGGCAGAGTTTTGATTTCCCATCGGATACATTGTTAGCGGGGATGAAGCTTGGATGGGATTTCAGAACCGGGCTCAACAAGTTCTTGACTTCTTGGAAAAATGCTAGTGATCCATCTCTTGGTGAGTATACCTATAGGATTGTTAATCTTGCTTTGCCTCAGCTTGTTCTTGCTAAGGGATCGGAGAAAGAGTTTCGAAGTGGGCCCTGGAATGGTCTTCGGTTTACTGGTGTTCCTGTTTCAAACAATGAAATTATAACACCTAGTTATGTGTATAATACAAACGAGTTGTATTATACGTATGAGACTAATGACAATTCTCTTATTACAAGATTGCAGCTGACTGAAGGGGGTGACATGAAGCGGTTAGTGCTGAATGAAGGAAGCACTGAATGGAACGATATGTATAATTTGCTGAATGATCGATGTGATAGTTACGGGGAGTGTGGCGCAAATGGCATTTGCAGAATTAACAACTCACCCATTTGTGACTGCTTGGAGGGGTTTATTCCGAAATCCCAGCAAGAGTGGGAGGTGCTTAATACGACAAGTGGGTGCACGAGGAAGATACAATTGGATTGCCAGAAGGGAGAAGGGTTTCGGAAAATTCAAAATGTAAAACTACCAGACCTGTTGGATTTTTGGGTCAACAAGAACCTGAATATGAAGGAATGCAGGGCAGAGTGCTTGAAGAATTGTTCTTGTATTGCTTATGCTTATTCAGATTACAGTCTTGGAGGCACCGGCTGTCTGATGTGGTTTAAAGACCTTATTGATATCCGAGAGTTTGTTGATGAAAGTGAGCAACATATTTATATTAGGATGCCAGCATCAGAACTAGGTCATGCCGGTCAGAAGGAGAAAAGAACAGTGCTCATAGCAGTCATCTCAGCAGCTTGTGTTCTAATTTTGCTGGGTGTGTCATGTTGGCTTATAATTTGGAAGAAGAAAGGAAGCGAGAGGGGTTCAAGATCGAATAAGGAAGACATGGAGTTGCCACTGTTTGATTTTCATACAATTGCAGCAGGCACCAACAACTTCTCAGGGACAAACATACTTGGGGAGGGAGGTTTTGGTCCAGTTTACAAGGTAAGAAATCGACAGTTGATCTCATTGTCTATCAATGTAATCTTAAAACTTACAGTAATTTTCAGGCCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

530

Amino Acids

59.36

Weight (kDa)

5.81

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 176 8.1e-35 D-mannose binding lectin
S_locus_glycop PF00954 208 - 316 1.7e-28 S-locus glycoprotein domain
PAN_2 PF08276 337 - 403 4.8e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 686, 1364
AccB1I GGYRCC 2 cut(s) 1169, 1457
AccB7I CCANNNNNTGG 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 557
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 4 cut(s) 103, 410, 467, 793
AclWI GGATC 3 cut(s) 347, 533, 616
AcsI RAATTY 3 cut(s) 186, 382, 1035
AcuI CTGAAG 1 cut(s) 795
AfaI GTAC 2 cut(s) 194, 286
AfiI CCNNNNNNNGG 3 cut(s) 248, 548, 641
AgsI TTSAA 6 cut(s) 140, 350, 672, 1040, 1120, 1398
AjnI CCWGG 2 cut(s) 247, 635
AjuI GAANNNNNNNTTGG 4 cut(s) 235, 267, 986, 1018
AluBI AGCT 5 cut(s) 367, 478, 592, 769, 1325
AluI AGCT 5 cut(s) 367, 478, 592, 769, 1325
Alw21I GWGCWC 2 cut(s) 989, 1305
Alw26I GTCTC 3 cut(s) 368, 390, 728
Alw44I GTGCAC 1 cut(s) 985
AlwI GGATC 3 cut(s) 347, 533, 616
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 3 cut(s) 7, 632, 1583
ApaI GGGCCC 1 cut(s) 636
ApaLI GTGCAC 1 cut(s) 985
ApeKI GCWGC 3 cut(s) 766, 1322, 1451
ApoI RAATTY 3 cut(s) 186, 382, 1035
Asp700I GAANNNNTTC 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 5 cut(s) 7, 292, 632, 633, 1505
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 4 cut(s) 91, 563, 794, 900
AsuII TTCGAA 2 cut(s) 206, 625
AvaII GGWCC 2 cut(s) 292, 1505
BaeGI GKGCMC 2 cut(s) 636, 989
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BanI GGYRCC 2 cut(s) 1169, 1457
BanII GRGCYC 3 cut(s) 265, 504, 636
BauI CACGAG 1 cut(s) 988
BbsI GAAGAC 2 cut(s) 638, 1419
Bbv12I GWGCWC 2 cut(s) 989, 1305
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 778, 1334, 1463
BccI CCATC 5 cut(s) 104, 251, 457, 477, 550
BciT130I CCWGG 2 cut(s) 249, 637
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 3 cut(s) 368, 390, 728
BfaI CTAG 4 cut(s) 534, 693, 1268, 1591
BfmI CTRYAG 2 cut(s) 405, 562
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 3 cut(s) 767, 1323, 1452
BlsI GCNGC 3 cut(s) 768, 1324, 1453
Bme1390I CCNGG 3 cut(s) 249, 499, 637
Bme18I GGWCC 2 cut(s) 292, 1505
BmgT120I GGNCC 5 cut(s) 7, 292, 632, 633, 1505
BmiI GGNNCC 4 cut(s) 262, 634, 1171, 1459
BmrFI CCNGG 3 cut(s) 249, 499, 637
BmsI GCATC 2 cut(s) 1242, 1268
BpiI GAAGAC 2 cut(s) 638, 1419
Bpu10I CCTNAGC 2 cut(s) 588, 603
Bpu14I TTCGAA 2 cut(s) 206, 625
BpuMI CCSGG 1 cut(s) 499
Bsa29I ATCGAT 1 cut(s) 853
BsaAI YACGTR 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 114, 635
BsaWI WCCGGW 2 cut(s) 125, 158
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 605, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 548, 641
Bse118I RCCGGY 2 cut(s) 1172, 1277
Bse1I ACTGG 3 cut(s) 382, 661, 1508
BseAI TCCGGA 1 cut(s) 158
BseBI CCWGG 2 cut(s) 249, 637
BseCI ATCGAT 1 cut(s) 853
BseDI CCNNGG 2 cut(s) 114, 635
BseGI GGATG 3 cut(s) 477, 488, 1257
BseLI CCNNNNNNNGG 3 cut(s) 248, 548, 641
BseMII CTCAG 3 cut(s) 602, 1332, 1485
BseNI ACTGG 3 cut(s) 382, 661, 1508
BseSI GKGCMC 2 cut(s) 636, 989
BseXI GCAGC 3 cut(s) 778, 1334, 1463
BseYI CCCAGC 2 cut(s) 948, 1342
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 3 cut(s) 9, 634, 1585
BshNI GGYRCC 2 cut(s) 1169, 1457
BshVI ATCGAT 1 cut(s) 853
BsiHKAI GWGCWC 2 cut(s) 989, 1305
BsiSI CCGG 5 cut(s) 126, 159, 498, 1173, 1278
BslFI GGGAC 1 cut(s) 1489
BslI CCNNNNNNNGG 3 cut(s) 248, 548, 641
BsmAI GTCTC 3 cut(s) 368, 390, 728
BsmFI GGGAC 1 cut(s) 1489
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 3 cut(s) 9, 634, 1585
Bsp119I TTCGAA 2 cut(s) 206, 625
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 5 cut(s) 265, 504, 636, 989, 1305
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
BspACI CCGC 4 cut(s) 103, 410, 467, 793
BspANI GGCC 3 cut(s) 9, 634, 1585
BspCNI CTCAG 3 cut(s) 601, 1331, 1484
BspDI ATCGAT 1 cut(s) 853
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 4 cut(s) 262, 634, 1171, 1459
BspPI GGATC 3 cut(s) 347, 533, 616
BspT104I TTCGAA 2 cut(s) 206, 625
BspT107I GGYRCC 2 cut(s) 1169, 1457
BsrBI CCGCTC 1 cut(s) 105
BsrFI RCCGGY 2 cut(s) 1172, 1277
BsrI ACTGG 3 cut(s) 382, 661, 1508
BssAI RCCGGY 2 cut(s) 1172, 1277
BssECI CCNNGG 2 cut(s) 114, 635
BssMI GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
BssNAI GTATAC 1 cut(s) 558
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 114
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 2 cut(s) 249, 637
Bst4CI ACNGT 8 cut(s) 217, 319, 343, 1160, 1300, 1431, 1533, 1573
Bst6I CTCTTC 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 729
BstBI TTCGAA 2 cut(s) 206, 625
BstC8I GCNNGC 2 cut(s) 1257, 1456
BstDEI CTNAG 4 cut(s) 588, 603, 1318, 1471
BstF5I GGATG 3 cut(s) 477, 488, 1257
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 6 cut(s) 355, 541, 611, 853, 1403, 1539
BstMAI GTCTC 3 cut(s) 368, 390, 728
BstMBI GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
BstMWI GCNNNNNNNGC 2 cut(s) 799, 885
BstNI CCWGG 2 cut(s) 249, 637
BstSCI CCNGG 3 cut(s) 247, 497, 635
BstSFI CTRYAG 2 cut(s) 405, 562
BstSLI GKGCMC 2 cut(s) 636, 989
BstSNI TACGTA 1 cut(s) 729
BstV1I GCAGC 3 cut(s) 778, 1334, 1463
BstV2I GAAGAC 2 cut(s) 638, 1419
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 1 cut(s) 352
BstZ17I GTATAC 1 cut(s) 558
Bsu15I ATCGAT 1 cut(s) 853
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 3 cut(s) 9, 634, 1585
BsuTUI ATCGAT 1 cut(s) 853
BtsCI GGATG 3 cut(s) 477, 488, 1257
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 5 cut(s) 70, 315, 816, 1305, 1427
Cac8I GCNNGC 2 cut(s) 1257, 1456
CfoI GCGC 1 cut(s) 881
Cfr10I RCCGGY 2 cut(s) 1172, 1277
Cfr13I GGNCC 5 cut(s) 7, 292, 632, 633, 1505
ClaI ATCGAT 1 cut(s) 853
Csp6I GTAC 2 cut(s) 193, 285
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 6 cut(s) 41, 174, 787, 1274, 1353, 1417
CviQI GTAC 2 cut(s) 193, 285
DdeI CTNAG 4 cut(s) 588, 603, 1318, 1471
DpnI GATC 6 cut(s) 354, 540, 610, 852, 1402, 1538
DpnII GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
DraI TTTAAA 1 cut(s) 1192
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Eco105I TACGTA 1 cut(s) 729
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 3 cut(s) 265, 504, 636
Eco32I GATATC 1 cut(s) 1207
Eco47I GGWCC 2 cut(s) 292, 1505
Eco57I CTGAAG 1 cut(s) 795
EcoO109I RGGNCCY 2 cut(s) 7, 633
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 247, 635
EcoRV GATATC 1 cut(s) 1207
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 3 cut(s) 265, 504, 636
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 6 cut(s) 44, 177, 790, 1277, 1356, 1420
FaqI GGGAC 1 cut(s) 1489
FatI CATG 6 cut(s) 40, 173, 786, 1273, 1352, 1416
FauI CCCGC 3 cut(s) 110, 403, 460
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 3 cut(s) 767, 1323, 1452
FokI GGATG 3 cut(s) 484, 495, 1264
FriOI GRGCYC 3 cut(s) 265, 504, 636
Fsp4HI GCNGC 3 cut(s) 767, 1323, 1452
FspBI CTAG 4 cut(s) 534, 693, 1268, 1591
GlaI GCGC 1 cut(s) 880
GluI GCNGC 3 cut(s) 767, 1323, 1452
GsaI CCCAGC 2 cut(s) 952, 1346
HaeIII GGCC 3 cut(s) 9, 634, 1585
HapII CCGG 5 cut(s) 126, 159, 498, 1173, 1278
HhaI GCGC 1 cut(s) 881
Hin1II CATG 6 cut(s) 44, 177, 790, 1277, 1356, 1420
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 1 cut(s) 147
HpaII CCGG 5 cut(s) 126, 159, 498, 1173, 1278
HphI GGTGA 4 cut(s) 91, 563, 794, 900
Hpy166II GTNNAC 6 cut(s) 195, 558, 654, 987, 1078, 1513
Hpy188III TCNNGA 3 cut(s) 159, 514, 1398
Hpy8I GTNNAC 6 cut(s) 195, 558, 654, 987, 1078, 1513
HpyAV CCTTC 7 cut(s) 20, 770, 803, 1009, 1016, 1090, 1279
HpyCH4III ACNGT 8 cut(s) 217, 319, 343, 1160, 1300, 1431, 1533, 1573
HpyCH4IV ACGT 1 cut(s) 728
HpyCH4V TGCA 8 cut(s) 63, 68, 362, 766, 894, 987, 1104, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 799, 885
HpyF3I CTNAG 4 cut(s) 588, 603, 1318, 1471
HpySE526I ACGT 1 cut(s) 728
Hsp92II CATG 6 cut(s) 44, 177, 790, 1277, 1356, 1420
HspAI GCGC 1 cut(s) 879
Kpn2I TCCGGA 1 cut(s) 158
Kzo9I GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
LmnI GCTCC 2 cut(s) 110, 260
Lsp1109I GCAGC 3 cut(s) 778, 1334, 1463
LweI GCATC 2 cut(s) 1242, 1268
MaeI CTAG 4 cut(s) 534, 693, 1268, 1591
MaeII ACGT 1 cut(s) 728
MaeIII GTNAC 3 cut(s) 782, 863, 917
MalI GATC 6 cut(s) 354, 540, 610, 852, 1402, 1538
MbiI CCGCTC 1 cut(s) 105
MboI GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
MfeI CAATTG 2 cut(s) 1001, 1446
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 5 cut(s) 265, 504, 636, 989, 1305
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 2 cut(s) 110, 1044
MnlI CCTC 9 cut(s) 166, 597, 922, 955, 984, 1160, 1383, 1486, 1490
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 1026
MseI TTAA 6 cut(s) 272, 573, 900, 969, 1191, 1562
MspA1I CMGCKG 2 cut(s) 410, 769
MspI CCGG 5 cut(s) 126, 159, 498, 1173, 1278
MspR9I CCNGG 3 cut(s) 249, 499, 637
MunI CAATTG 2 cut(s) 1001, 1446
Mva1269I GAATGC 1 cut(s) 1106
MvaI CCWGG 2 cut(s) 249, 637
MwoI GCNNNNNNNGC 2 cut(s) 799, 885
NciI CCSGG 1 cut(s) 499
NdeII GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
NlaIII CATG 6 cut(s) 44, 177, 790, 1277, 1356, 1420
NlaIV GGNNCC 4 cut(s) 262, 634, 1171, 1459
NmuCI GTSAC 2 cut(s) 782, 917
NspV TTCGAA 2 cut(s) 206, 625
PctI GAATGC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 1026
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 3 cut(s) 768, 1324, 1453
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 729
PsiI TTATAA 2 cut(s) 686, 1364
Psp6I CCWGG 2 cut(s) 247, 635
PspFI CCCAGC 2 cut(s) 948, 1342
PspGI CCWGG 2 cut(s) 247, 635
PspN4I GGNNCC 4 cut(s) 262, 634, 1171, 1459
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 5 cut(s) 7, 292, 632, 633, 1505
PsuI RGATCY 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 2 cut(s) 194, 286
RsaNI GTAC 2 cut(s) 193, 285
SaqAI TTAA 6 cut(s) 272, 573, 900, 969, 1191, 1562
SatI GCNGC 3 cut(s) 767, 1323, 1452
Sau3AI GATC 6 cut(s) 352, 538, 608, 850, 1400, 1536
Sau96I GGNCC 5 cut(s) 7, 292, 632, 633, 1505
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 3 cut(s) 249, 499, 637
SduI GDGCHC 5 cut(s) 265, 504, 636, 989, 1305
SfaNI GCATC 2 cut(s) 1242, 1268
SfcI CTRYAG 2 cut(s) 405, 562
SfuI TTCGAA 2 cut(s) 206, 625
SinI GGWCC 2 cut(s) 292, 1505
SnaBI TACGTA 1 cut(s) 729
SsiI CCGC 4 cut(s) 103, 410, 467, 793
SspMI CTAG 4 cut(s) 534, 693, 1268, 1591
StyD4I CCNGG 3 cut(s) 247, 497, 635
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 8 cut(s) 217, 319, 343, 1160, 1300, 1431, 1533, 1573
TaiI ACGT 1 cut(s) 731
TaqI TCGA 5 cut(s) 206, 625, 853, 1403, 1528
Tru1I TTAA 6 cut(s) 272, 573, 900, 969, 1191, 1562
Tru9I TTAA 6 cut(s) 272, 573, 900, 969, 1191, 1562
TscAI CASTG 5 cut(s) 70, 322, 823, 1305, 1434
TseFI GTSAC 2 cut(s) 782, 917
TseI GCWGC 3 cut(s) 766, 1322, 1451
Tsp45I GTSAC 2 cut(s) 782, 917
TspRI CASTG 5 cut(s) 70, 322, 823, 1305, 1434
Van91I CCANNNNNTGG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 985
VpaK11BI GGWCC 2 cut(s) 292, 1505
XapI RAATTY 3 cut(s) 186, 382, 1035
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 4 cut(s) 534, 693, 1268, 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.