Rroxscaffold_1G00021030

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001151
Physical Location & Seq
Forward (+)
25673739 .. 25677737
3999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00021030.1

Sequence Viewer

Length: 1383 bp
ATGAAGGGCCTCATCTTCATCTTCATCTCTCTATTCCTATTTTTAGCCTTGAATTTCTCCACTGCAGCTGATACCATATCTCCATCACAATCCATCACCGGCTCGAATACCTTGGTTTCTTCCAACCAAAGATTCGAATTAGGACTCTTCTCTCCACCCAATTCTAGAGCATGGTATTTAGGACTATGGTACAAGAAAACTCCACATGTTGTTGTATGGATTGCAAACAGAGAAAACCCACTTCCAGGTTCAAATGGAGCCTTGATATTATCAGAAAAGGGCACTCTCATCCTTTCAGACCAAATGAACAACACAATTTGGTCCTCAAATTCTTCCAGAGTAGCCGCAAACCCAGTTGCGCAGCTTTTGGTGACCGGAAATCTTGTTGTTAGAGACCAGGCGTCTCCAACAGCAACAGCAGAGTCCGAAAGTTATGTATTTCAAAGCTTTGATTTCCCATCAAACACGCTTTTACCAGATATGAAGGTAGGGTGGGACTTCAAAACTGGCCAAAACCATTTCTTGACTAGTTGGAAAAATGCTAGCGATCCCTCCCTTGGAGAATATACCTATAGGATTGATAACCCTGAGTTGCCTCAGCTTGTTGTTGCTCAAGGATCAAAGAAAGTGTTCAGGACAGGGCCTTGGAATGGTCTTCGATTTACAGGTACTCCTGTTTCGGTTGTTCCAACAGCATCATTTTTGGTACCAATCTTTGTCTACAATACCAAGGAGTTGTATTACACATATGAGGTTACTGATGATTCTATTCTGACAAGAGTGACGCTGACTGAGTCGGGTTTGGCTCAGCGGCTAGTGATGAATGAAGGGAGCAGTGAATGGACTGTTATGTACACTCTGCAGAACAGTCAGTGTGATGATTATGGTAGGTGTGGGGCAAATAGCATTTGCAAGATTTACAAATCACCGATTTGTGAATGCTTGCAGGGGTTTGTTCCCAAATCTCCAAAGGAATGGGAGATTCTTAACTGGACAAGTGGGTGCACGAGGAGGACAGCACTGCTTTGTGAGAAAGGGGAAGGATTTGTGAAAGTTAATAATGTGAAACTGCCGGACTTGTTGGATTTTCGGGTGAACAAAACCATGCTCCGCGGAGAGGAATGTGAGGCAGAGTGTTTGAGGAACTGCTCCTGTATGGCTCATGCCAATTCGAACATAACCAATGGAGGCAAAGGATGTCTTATGTGGTTTGGCAAGCTGATTGATATGCGGGAGATCGCTGAAGATAGTGACCAGATATATATTCGGATGCCAGCTTCCGAACTAGCCAGAAACATAAAAGAGTATTGCTCGTCTCGGTCATATCATCAGCAGGTTTTGTGCTTCTTTTTCTGGGTTTCTCATGCTGCTATATTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

460

Amino Acids

51.58

Weight (kDa)

6.55

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 179 2.3e-32 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 2.5e-27 S-locus glycoprotein domain
PAN_2 PF08276 343 - 410 4.8e-19 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 1324
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 1113
AciI CCGC 5 cut(s) 345, 811, 1111, 1113, 1231
AclWI GGATC 2 cut(s) 542, 625
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 2 cut(s) 52, 328
AcuI CTGAAG 1 cut(s) 1263
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 4 cut(s) 191, 670, 708, 854
AfiI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 4 cut(s) 52, 252, 443, 502
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 2 cut(s) 244, 396
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AluBI AGCT 6 cut(s) 68, 364, 447, 601, 1219, 1277
AluI AGCT 6 cut(s) 68, 364, 447, 601, 1219, 1277
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 3 cut(s) 387, 408, 1320
Alw44I GTGCAC 1 cut(s) 1003
AlwI GGATC 2 cut(s) 542, 625
AoxI GGCC 3 cut(s) 7, 508, 641
ApaLI GTGCAC 1 cut(s) 1003
ApeKI GCWGC 3 cut(s) 65, 361, 1367
ApoI RAATTY 2 cut(s) 52, 328
Asp700I GAANNNNTTC 1 cut(s) 629
Asp718I GGTACC 1 cut(s) 706
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 3 cut(s) 7, 321, 641
AsuHPI GGTGA 4 cut(s) 88, 382, 918, 1105
AsuII TTCGAA 2 cut(s) 135, 1172
AsuNHI GCTAGC 1 cut(s) 542
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 284, 1007
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 1 cut(s) 706
BauI CACGAG 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 647
Bbv12I GWGCWC 1 cut(s) 1007
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 77, 373, 1354
BccI CCATC 3 cut(s) 91, 101, 466
BciT130I CCWGG 2 cut(s) 246, 398
BcoDI GTCTC 3 cut(s) 387, 408, 1320
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 5 cut(s) 165, 528, 543, 815, 1286
BfmI CTRYAG 3 cut(s) 63, 571, 860
BfuAI ACCTGC 1 cut(s) 1324
BisI GCNGC 5 cut(s) 66, 345, 362, 812, 1368
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 5 cut(s) 67, 346, 363, 813, 1369
Bme1390I CCNGG 2 cut(s) 246, 398
Bme18I GGWCC 1 cut(s) 321
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 7, 321, 641
BmiI GGNNCC 2 cut(s) 259, 708
BmrFI CCNGG 2 cut(s) 246, 398
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 2 cut(s) 704, 1260
BmtI GCTAGC 1 cut(s) 546
BmuI ACTGGG 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 647
BplI GAGNNNNNCTC 2 cut(s) 1295, 1327
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 2 cut(s) 135, 1172
BpuEI CTTGAG 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 655, 685
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 353, 511, 995
BseBI CCWGG 2 cut(s) 246, 398
BseDI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BseGI GGATG 3 cut(s) 288, 1202, 1275
BseLI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
BseMII CTCAG 4 cut(s) 579, 611, 783, 821
BseNI ACTGG 3 cut(s) 353, 511, 995
BseRI GAGGAG 1 cut(s) 1024
BseSI GKGCMC 2 cut(s) 284, 1007
BseXI GCAGC 3 cut(s) 77, 373, 1354
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 3 cut(s) 9, 510, 643
BshNI GGYRCC 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 1007
BsiSI CCGG 3 cut(s) 99, 375, 1073
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
BsmAI GTCTC 3 cut(s) 387, 408, 1320
BsmBI CGTCTC 2 cut(s) 408, 1320
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 3 cut(s) 9, 510, 643
Bso31I GGTCTC 1 cut(s) 387
Bsp119I TTCGAA 2 cut(s) 135, 1172
Bsp1286I GDGCHC 2 cut(s) 284, 1007
Bsp1407I TGTACA 1 cut(s) 852
Bsp143I GATC 3 cut(s) 547, 617, 1236
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 5 cut(s) 345, 811, 1111, 1113, 1231
BspANI GGCC 3 cut(s) 9, 510, 643
BspCNI CTCAG 4 cut(s) 580, 610, 784, 820
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 2 cut(s) 259, 708
BspMAI CTGCAG 2 cut(s) 67, 864
BspMI ACCTGC 1 cut(s) 1324
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 542, 625
BspT104I TTCGAA 2 cut(s) 135, 1172
BspT107I GGYRCC 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 852
BsrI ACTGG 3 cut(s) 353, 511, 995
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 5 cut(s) 111, 556, 644, 729, 1111
BssMI GATC 3 cut(s) 547, 617, 1236
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 1006
BssT1I CCWWGG 4 cut(s) 111, 556, 644, 729
Bst2BI CACGAG 1 cut(s) 1006
Bst2UI CCWGG 2 cut(s) 246, 398
Bst4CI ACNGT 2 cut(s) 847, 869
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 401
BstAUI TGTACA 1 cut(s) 852
BstBI TTCGAA 2 cut(s) 135, 1172
BstC8I GCNNGC 4 cut(s) 544, 944, 1217, 1275
BstDEI CTNAG 4 cut(s) 588, 597, 792, 807
BstDSI CCRYGG 1 cut(s) 1111
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 3 cut(s) 288, 1202, 1275
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 550, 620, 1239
BstMAI GTCTC 3 cut(s) 387, 408, 1320
BstMBI GATC 3 cut(s) 547, 617, 1236
BstNI CCWGG 2 cut(s) 246, 398
BstNSI RCATGY 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 370
BstSCI CCNGG 2 cut(s) 244, 396
BstSFI CTRYAG 3 cut(s) 63, 571, 860
BstSLI GKGCMC 2 cut(s) 284, 1007
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 3 cut(s) 77, 373, 1354
BstV2I GAAGAC 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 975
BsuRI GGCC 3 cut(s) 9, 510, 643
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 3 cut(s) 288, 1202, 1275
BtsI GCAGTG 3 cut(s) 60, 841, 1019
BtsIMutI CAGTG 4 cut(s) 60, 841, 878, 1019
BveI ACCTGC 1 cut(s) 1324
Cac8I GCNNGC 4 cut(s) 544, 944, 1217, 1275
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 7, 321, 641
Cfr42I CCGCGG 1 cut(s) 1114
CseI GACGC 2 cut(s) 390, 793
Csp6I GTAC 4 cut(s) 190, 669, 707, 853
CviAII CATG 5 cut(s) 171, 206, 1105, 1163, 1364
CviQI GTAC 4 cut(s) 190, 669, 707, 853
DdeI CTNAG 4 cut(s) 588, 597, 792, 807
DpnI GATC 3 cut(s) 549, 619, 1238
DpnII GATC 3 cut(s) 547, 617, 1236
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 152
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 4 cut(s) 111, 556, 644, 729
Eco31I GGTCTC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 1263
Eco91I GGTNACC 1 cut(s) 370
EcoO109I RGGNCCY 2 cut(s) 7, 641
EcoO65I GGTNACC 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 244, 396
EcoT14I CCWWGG 4 cut(s) 111, 556, 644, 729
ErhI CCWWGG 4 cut(s) 111, 556, 644, 729
Esp3I CGTCTC 2 cut(s) 408, 1320
FaeI CATG 5 cut(s) 174, 209, 1108, 1166, 1367
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 1 cut(s) 509
FatI CATG 5 cut(s) 170, 205, 1104, 1162, 1363
FauI CCCGC 1 cut(s) 1224
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 5 cut(s) 66, 345, 362, 812, 1368
FokI GGATG 3 cut(s) 275, 1209, 1282
Fsp4HI GCNGC 5 cut(s) 66, 345, 362, 812, 1368
FspBI CTAG 5 cut(s) 165, 528, 543, 815, 1286
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GluI GCNGC 5 cut(s) 66, 345, 362, 812, 1368
HaeIII GGCC 3 cut(s) 9, 510, 643
HapII CCGG 3 cut(s) 99, 375, 1073
HgaI GACGC 2 cut(s) 390, 793
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 5 cut(s) 174, 209, 1108, 1166, 1367
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 6 cut(s) 132, 144, 422, 764, 794, 982
HpaII CCGG 3 cut(s) 99, 375, 1073
HphI GGTGA 4 cut(s) 88, 382, 918, 1105
Hpy166II GTNNAC 4 cut(s) 721, 855, 1005, 1096
Hpy188I TCNGA 6 cut(s) 274, 298, 427, 774, 1269, 1282
Hpy188III TCNNGA 4 cut(s) 165, 336, 523, 634
Hpy8I GTNNAC 4 cut(s) 721, 855, 1005, 1096
HpyAV CCTTC 3 cut(s) 478, 821, 1034
HpyCH4III ACNGT 2 cut(s) 847, 869
HpyCH4V TGCA 6 cut(s) 65, 224, 862, 912, 946, 1005
HpyF3I CTNAG 4 cut(s) 588, 597, 792, 807
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 5 cut(s) 174, 209, 1108, 1166, 1367
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 710
KspI CCGCGG 1 cut(s) 1114
Kzo9I GATC 3 cut(s) 547, 617, 1236
LmnI GCTCC 4 cut(s) 257, 831, 1113, 1154
Lsp1109I GCAGC 3 cut(s) 77, 373, 1354
LweI GCATC 2 cut(s) 704, 1260
MaeI CTAG 5 cut(s) 165, 528, 543, 815, 1286
MaeIII GTNAC 4 cut(s) 370, 754, 781, 1250
MalI GATC 3 cut(s) 549, 619, 1238
MboI GATC 3 cut(s) 547, 617, 1236
MboII GAAGA 7 cut(s) 7, 13, 111, 139, 324, 647, 1256
MhlI GDGCHC 2 cut(s) 284, 1007
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 6 cut(s) 52, 137, 160, 315, 328, 1168
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 3 cut(s) 138, 431, 803
MmeI TCCRAC 5 cut(s) 147, 431, 512, 713, 1062
Mox20I TGGCCA 1 cut(s) 510
MroXI GAANNNNTTC 1 cut(s) 629
MscI TGGCCA 1 cut(s) 510
MseI TTAA 2 cut(s) 987, 1056
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 68, 811, 1113
MspI CCGG 3 cut(s) 99, 375, 1073
MspR9I CCNGG 2 cut(s) 246, 398
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 2 cut(s) 246, 398
MvnI CGCG 1 cut(s) 1113
NdeI CATATG 1 cut(s) 748
NdeII GATC 3 cut(s) 547, 617, 1236
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 5 cut(s) 174, 209, 1108, 1166, 1367
NlaIV GGNNCC 2 cut(s) 259, 708
NmuCI GTSAC 3 cut(s) 370, 781, 1250
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 209
NspV TTCGAA 2 cut(s) 135, 1172
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 3 cut(s) 132, 764, 982
PflFI GACNNNGTC 1 cut(s) 793
PkrI GCNGC 5 cut(s) 67, 346, 363, 813, 1369
PleI GAGTC 3 cut(s) 138, 430, 802
PpsI GAGTC 3 cut(s) 138, 430, 802
PscI ACATGT 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 244, 396
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 2 cut(s) 244, 396
PspN4I GGNNCC 2 cut(s) 259, 708
PspPI GGNCC 3 cut(s) 7, 321, 641
PstI CTGCAG 2 cut(s) 67, 864
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 4 cut(s) 191, 670, 708, 854
RsaNI GTAC 4 cut(s) 190, 669, 707, 853
SacII CCGCGG 1 cut(s) 1114
SaqAI TTAA 2 cut(s) 987, 1056
SatI GCNGC 5 cut(s) 66, 345, 362, 812, 1368
Sau3AI GATC 3 cut(s) 547, 617, 1236
Sau96I GGNCC 3 cut(s) 7, 321, 641
SchI GAGTC 3 cut(s) 138, 431, 803
ScrFI CCNGG 2 cut(s) 246, 398
SduI GDGCHC 2 cut(s) 284, 1007
SfaNI GCATC 2 cut(s) 704, 1260
SfcI CTRYAG 3 cut(s) 63, 571, 860
Sfr303I CCGCGG 1 cut(s) 1114
SfuI TTCGAA 2 cut(s) 135, 1172
SgrBI CCGCGG 1 cut(s) 1114
SinI GGWCC 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 6 cut(s) 52, 137, 160, 315, 328, 1168
SsiI CCGC 5 cut(s) 345, 811, 1111, 1113, 1231
SspMI CTAG 5 cut(s) 165, 528, 543, 815, 1286
StyD4I CCNGG 2 cut(s) 244, 396
StyI CCWWGG 4 cut(s) 111, 556, 644, 729
TaaI ACNGT 2 cut(s) 847, 869
TaqI TCGA 4 cut(s) 104, 135, 658, 1172
TaqII GACCGA 1 cut(s) 1308
TasI AATT 6 cut(s) 52, 137, 160, 315, 328, 1168
TatI WGTACW 1 cut(s) 852
TauI GCSGC 2 cut(s) 347, 814
TfiI GAWTC 3 cut(s) 132, 764, 982
Tru1I TTAA 2 cut(s) 987, 1056
Tru9I TTAA 2 cut(s) 987, 1056
TscAI CASTG 4 cut(s) 67, 841, 878, 1026
TseFI GTSAC 3 cut(s) 370, 781, 1250
TseI GCWGC 3 cut(s) 65, 361, 1367
Tsp45I GTSAC 3 cut(s) 370, 781, 1250
TspDTI ATGAA 7 cut(s) 7, 13, 17, 320, 497, 836, 840
TspRI CASTG 4 cut(s) 67, 841, 878, 1026
Tth111I GACNNNGTC 1 cut(s) 793
VneI GTGCAC 1 cut(s) 1003
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 2 cut(s) 52, 328
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 5 cut(s) 165, 528, 543, 815, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.