RchiOBHm_Chr5g0036361

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001151
Physical Location & Seq
Forward (+)
30553155 .. 30554662
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31518

Sequence Viewer

Length: 1038 bp
ATGCATATTCATTGCTTTTATAATATGGTCTTCGTCCATTTTATTTATTTTATTGTTCTTCTTTGTTTTACAGGTTCAAGAGCTAGTAAGGAGGACTTGGAGTTGCCCCAGTTTGATTTTCATACTATTGCAACTGCCACCGATAACTTCTCCAGCAGAAATAAACTTGGAGAGGGAGGTTTGGGTCCAGTTTACAAGGCCAAACTAACACAAGAAGAATCAATAGCAGTAAAGAGACTCTCAAAACACTCTGGGCAAGGTGTTGAAGAGTTTAAGAATGAAGTTACGATGATAGCCAATCTTCAACACCGGAACCTGGTTAAGCTTTTGGGCTGCTGCATTGAAGGAAAAGAAAGGATGCTAATCTACGAGTACATGCCCAACAAAAGCTTGGACTTCTTCATCTTCGATCAAAATAGAAAGGTGGTGTTGAGTTGGCAAAAGCGATTTGACATTATCATGGGGCTTGCAAGAGGATTACTCTACCTCCACCAAGACTCCAGAGTCAGAATCATTCACAGGGACCTTAAGAGTAGCAACATCCTACTTGACGATGAGCTGATGAACCCCAAAATATCCGACTTTGGCATAGCAAGAATTTTCAGAAACAATCAAAGTGAAGCTAAAACCAAACGAGTAATTGGCACATATGGTTATTTGTCTCCTGAGTATGCAATTGATGGCAAGTTTTCAGTGAAATCTGATGTCTTCAGTTTTGGAGTGATTCTGTTGGAGATAATAAGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGACCACTATCACAGTCTTCTGGGACACTTCGAAGTGCTAAGATGCATTCAAGTGGGTCTTTCGTGCGTTCAAAAGCTGCCGAAAGACAGACCAGAAATGTCATCAGTGCTTCCTATGTTGAGCAATGAGGGTGTAGCATTGCCTCAACCAAAGGAGCCTGGTTTCTTCACAGAAAGAAGTTCTGTAGACATTGATACCATCATAGGTGAAGGAAGAAGTCACACAGGAACCACATTTACTATTACGATGGTAGAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

345

Amino Acids

39.37

Weight (kDa)

8.62

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 51 - 252 5.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 53 - 294 1.2e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 301 - 345 1.5e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AasI GACNNNNNNGTC 1 cut(s) 503
AccI GTMKAC 1 cut(s) 960
AcsI RAATTY 1 cut(s) 597
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 316
AflII CTTAAG 1 cut(s) 527
AgsI TTSAA 6 cut(s) 78, 266, 305, 344, 824, 845
AjnI CCWGG 2 cut(s) 315, 931
AluBI AGCT 7 cut(s) 83, 325, 390, 559, 623, 850, 1031
AluI AGCT 7 cut(s) 83, 325, 390, 559, 623, 850, 1031
Alw26I GTCTC 2 cut(s) 229, 666
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 3 cut(s) 333, 336, 850
ApoI RAATTY 1 cut(s) 597
AspS9I GGNCC 2 cut(s) 185, 523
AsuHPI GGTGA 1 cut(s) 992
AsuII TTCGAA 1 cut(s) 804
AvaII GGWCC 2 cut(s) 185, 523
BbsI GAAGAC 3 cut(s) 22, 700, 782
BbvI GCAGC 3 cut(s) 320, 323, 837
BccI CCATC 3 cut(s) 674, 980, 1015
BciT130I CCWGG 2 cut(s) 317, 933
BcoDI GTCTC 2 cut(s) 229, 666
BfaI CTAG 2 cut(s) 84, 1032
BfmI CTRYAG 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 527
BisI GCNGC 3 cut(s) 334, 337, 851
BlsI GCNGC 3 cut(s) 335, 338, 852
Bme1390I CCNGG 2 cut(s) 317, 933
Bme18I GGWCC 2 cut(s) 185, 523
BmgT120I GGNCC 2 cut(s) 185, 523
BmiI GGNNCC 5 cut(s) 186, 314, 524, 930, 1003
BmrFI CCNGG 2 cut(s) 317, 933
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 348, 806
BmuI ACTGGG 1 cut(s) 103
BpiI GAAGAC 3 cut(s) 22, 700, 782
BplI GAGNNNNNCTC 2 cut(s) 465, 497
BpmI CTGGAG 2 cut(s) 136, 484
Bpu14I TTCGAA 1 cut(s) 804
BsaBI GATNNNNATC 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 309
BsaXI ACNNNNNCTCC 4 cut(s) 471, 482, 501, 512
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 2 cut(s) 109, 188
Bse3DI GCAATG 3 cut(s) 10, 904, 911
Bse8I GATNNNNATC 1 cut(s) 362
BseBI CCWGG 2 cut(s) 317, 933
BseGI GGATG 3 cut(s) 363, 540, 769
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 3 cut(s) 10, 904, 911
BseMII CTCAG 1 cut(s) 657
BseNI ACTGG 2 cut(s) 109, 188
BseXI GCAGC 3 cut(s) 320, 323, 837
BshFI GGCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 2 cut(s) 536, 810
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 229, 666
BsmFI GGGAC 2 cut(s) 536, 810
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 804
Bsp143I GATC 1 cut(s) 409
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 658
BspLI GGNNCC 5 cut(s) 186, 314, 524, 930, 1003
BspT104I TTCGAA 1 cut(s) 804
BspTI CTTAAG 1 cut(s) 527
BsrDI GCAATG 3 cut(s) 10, 904, 911
BsrI ACTGG 2 cut(s) 109, 188
BssMI GATC 1 cut(s) 409
Bst2UI CCWGG 2 cut(s) 317, 933
Bst4CI ACNGT 1 cut(s) 788
Bst6I CTCTTC 1 cut(s) 261
BstAFI CTTAAG 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 2 cut(s) 666, 812
BstF5I GGATG 3 cut(s) 363, 540, 769
BstKTI GATC 1 cut(s) 412
BstMAI GTCTC 2 cut(s) 229, 666
BstMBI GATC 1 cut(s) 409
BstNI CCWGG 2 cut(s) 317, 933
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 315, 931
BstSFI CTRYAG 1 cut(s) 957
BstV1I GCAGC 3 cut(s) 320, 323, 837
BstV2I GAAGAC 3 cut(s) 22, 700, 782
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 3 cut(s) 363, 540, 769
BtsIMutI CAGTG 2 cut(s) 699, 885
Cac8I GCNNGC 1 cut(s) 468
Cfr13I GGNCC 2 cut(s) 185, 523
CsiI ACCWGGT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 373
CviAII CATG 2 cut(s) 376, 460
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 2 cut(s) 666, 812
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 185, 523
Eco57I CTGAAG 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 315, 931
EcoT22I ATGCAT 2 cut(s) 6, 821
FaeI CATG 2 cut(s) 379, 463
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 816, 848
FaqI GGGAC 2 cut(s) 536, 810
FatI CATG 2 cut(s) 375, 459
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 1 cut(s) 960
Fnu4HI GCNGC 3 cut(s) 334, 337, 851
FokI GGATG 3 cut(s) 370, 527, 756
Fsp4HI GCNGC 3 cut(s) 334, 337, 851
FspBI CTAG 2 cut(s) 84, 1032
GluI GCNGC 3 cut(s) 334, 337, 851
GsuI CTGGAG 2 cut(s) 136, 484
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 310
Hin1II CATG 2 cut(s) 379, 463
HindIII AAGCTT 2 cut(s) 323, 388
HinfI GANTC 6 cut(s) 218, 237, 497, 504, 510, 724
HpaII CCGG 1 cut(s) 310
HphI GGTGA 1 cut(s) 992
Hpy166II GTNNAC 2 cut(s) 193, 961
Hpy188I TCNGA 4 cut(s) 509, 580, 605, 703
Hpy188III TCNNGA 4 cut(s) 78, 501, 665, 773
Hpy8I GTNNAC 2 cut(s) 193, 961
HpyAV CCTTC 2 cut(s) 338, 977
HpyCH4III ACNGT 1 cut(s) 788
HpyCH4V TGCA 6 cut(s) 4, 131, 339, 470, 674, 819
HpyF3I CTNAG 2 cut(s) 666, 812
Hsp92II CATG 2 cut(s) 379, 463
Kzo9I GATC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 928
Lsp1109I GCAGC 3 cut(s) 320, 323, 837
LweI GCATC 2 cut(s) 348, 806
MabI ACCWGGT 1 cut(s) 315
MaeI CTAG 2 cut(s) 84, 1032
MaeIII GTNAC 2 cut(s) 283, 992
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MfeI CAATTG 1 cut(s) 675
MluCI AATT 3 cut(s) 597, 639, 675
MlyI GAGTC 3 cut(s) 231, 491, 513
MmeI TCCRAC 2 cut(s) 603, 711
MnlI CCTC 8 cut(s) 85, 166, 170, 467, 497, 752, 895, 927
Mph1103I ATGCAT 2 cut(s) 6, 821
MseI TTAA 3 cut(s) 273, 321, 528
MslI CAYNNNNRTG 2 cut(s) 458, 824
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 2 cut(s) 317, 933
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 2 cut(s) 317, 933
NdeI CATATG 1 cut(s) 649
NdeII GATC 1 cut(s) 409
NlaIII CATG 2 cut(s) 379, 463
NlaIV GGNNCC 5 cut(s) 186, 314, 524, 930, 1003
NmuCI GTSAC 1 cut(s) 992
NsiI ATGCAT 2 cut(s) 6, 821
NspI RCATGY 1 cut(s) 379
NspV TTCGAA 1 cut(s) 804
PctI GAATGC 1 cut(s) 819
PfeI GAWTC 3 cut(s) 218, 510, 724
PkrI GCNGC 3 cut(s) 335, 338, 852
PleI GAGTC 3 cut(s) 231, 491, 512
PpsI GAGTC 3 cut(s) 231, 491, 512
PpuMI RGGWCCY 1 cut(s) 523
PsiI TTATAA 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 315, 931
PspGI CCWGG 2 cut(s) 315, 931
PspN4I GGNNCC 5 cut(s) 186, 314, 524, 930, 1003
PspPI GGNCC 2 cut(s) 185, 523
PspPPI RGGWCCY 1 cut(s) 523
PsrI GAACNNNNNNTAC 2 cut(s) 994, 1026
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 2 cut(s) 458, 824
SaqAI TTAA 3 cut(s) 273, 321, 528
SatI GCNGC 3 cut(s) 334, 337, 851
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 2 cut(s) 185, 523
SchI GAGTC 3 cut(s) 231, 491, 513
ScrFI CCNGG 2 cut(s) 317, 933
SexAI ACCWGGT 1 cut(s) 315
SfaNI GCATC 2 cut(s) 348, 806
SfcI CTRYAG 1 cut(s) 957
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 2 cut(s) 185, 523
SmiMI CAYNNNNRTG 2 cut(s) 458, 824
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 3 cut(s) 597, 639, 675
SspMI CTAG 2 cut(s) 84, 1032
StyD4I CCNGG 2 cut(s) 315, 931
TaaI ACNGT 1 cut(s) 788
TaqI TCGA 2 cut(s) 408, 804
TasI AATT 3 cut(s) 597, 639, 675
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 3 cut(s) 218, 510, 724
Tru1I TTAA 3 cut(s) 273, 321, 528
Tru9I TTAA 3 cut(s) 273, 321, 528
TscAI CASTG 2 cut(s) 699, 885
TseFI GTSAC 1 cut(s) 992
TseI GCWGC 3 cut(s) 333, 336, 850
Tsp45I GTSAC 1 cut(s) 992
TspDTI ATGAA 5 cut(s) 110, 294, 391, 578, 755
TspRI CASTG 2 cut(s) 699, 885
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 2 cut(s) 185, 523
XapI RAATTY 1 cut(s) 597
XceI RCATGY 1 cut(s) 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 960
XspI CTAG 2 cut(s) 84, 1032
Zsp2I ATGCAT 2 cut(s) 6, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.