Rorug05G0323300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001151
Physical Location & Seq
Forward (+)
38803548 .. 38804084
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0323300.1

Sequence Viewer

Length: 537 bp
ATGTCTGCATCCCATTACCACTGGTACACCTCGGAGTTTGCCGACCCACCTCAAATCCTCCACGTATACGACACCGTTTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTCAGCCAACACCCTGCCGTTCTCCACGTCTTCGAAGACCGCCGCCGCCAGCTCCACACCACCCGCTCGCCGCAGTTCCTGGGCCTGAGAAACCAGAAGGGGCTGTGGTCGGAATCGGACTACGGCTCCGATGTCATCGTCAGGGTTTTCGATACTGGAGTTTGGCCGGAGCGTTGGAGCTTTTCCAATAGGAATGTCGGTCCGGTTCCAAAGCGGTGGAGAGGGGTCTGTGAGACCGGAGTTAAATTTACAGCTCGGAATTGCAATAAGAAGCTAATCGGAGCTCGGTTCTTCATCAAAGGTCATGAAGCCGCTGCCAATGCCGGCGGTCCGATAACCGCAATCAACGGCTCAGTTGAATGGCGGCGTGTAGGGAATTGGCGAGGGCGATTTGAAGTTTGGGGAGATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

178

Amino Acids

20.29

Weight (kDa)

9.69

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 4 - 60 2.6e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 287
AcsI RAATTY 1 cut(s) 368
AfaI GTAC 1 cut(s) 26
AgsI TTSAA 2 cut(s) 482, 518
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 5 cut(s) 175, 303, 377, 397, 407
AluI AGCT 5 cut(s) 175, 303, 377, 397, 407
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 202
AoxI GGCC 2 cut(s) 205, 287
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 368
AspS9I GGNCC 3 cut(s) 205, 323, 452
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 323, 452
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BanII GRGCYC 1 cut(s) 409
BbsI GAAGAC 2 cut(s) 145, 165
Bbv12I GWGCWC 1 cut(s) 409
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 424
BceAI ACGGC 3 cut(s) 125, 262, 487
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 350
BisI GCNGC 6 cut(s) 166, 169, 194, 435, 438, 488
BlsI GCNGC 6 cut(s) 167, 170, 195, 436, 439, 489
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 2 cut(s) 323, 452
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 3 cut(s) 205, 323, 452
BmiI GGNNCC 2 cut(s) 250, 330
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 145, 165
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 30, 82, 202
BsaWI WCCGGW 2 cut(s) 325, 359
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 26, 283
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 30, 82, 202
BseGI GGATG 1 cut(s) 8
BseMII CTCAG 3 cut(s) 139, 200, 489
BseNI ACTGG 2 cut(s) 26, 283
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 2 cut(s) 207, 289
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 4 cut(s) 290, 326, 360, 447
BsmAI GTCTC 1 cut(s) 350
BsnI GGCC 2 cut(s) 207, 289
Bso31I GGTCTC 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 409
Bsp19I CCATGG 1 cut(s) 82
BspANI GGCC 2 cut(s) 207, 289
BspCNI CTCAG 3 cut(s) 138, 201, 488
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 250, 330
BspT104I TTCGAA 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 2 cut(s) 26, 283
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 30, 82, 202
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 82
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 76
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 173, 191, 448
BstDEI CTNAG 3 cut(s) 125, 209, 475
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 8
BstMAI GTCTC 1 cut(s) 350
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 2 cut(s) 145, 165
BstXI CCANNNNNNTGG 1 cut(s) 339
BstZ17I GTATAC 1 cut(s) 67
BsuRI GGCC 2 cut(s) 207, 289
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 3 cut(s) 173, 191, 448
CaiI CAGNNNCTG 1 cut(s) 202
CciI TCATGA 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 3 cut(s) 205, 323, 452
CpoI CGGWCCG 2 cut(s) 323, 452
Csp6I GTAC 1 cut(s) 25
CspI CGGWCCG 2 cut(s) 323, 452
CviAII CATG 2 cut(s) 83, 428
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 125, 209, 475
EaeI YGGCCR 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 407
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 323, 452
Eco53kI GAGCTC 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 2 cut(s) 86, 431
FaiI YATR 4 cut(s) 67, 84, 429, 535
FatI CATG 2 cut(s) 82, 427
FauI CCCGC 1 cut(s) 194
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 166, 169, 194, 435, 438, 488
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 6 cut(s) 166, 169, 194, 435, 438, 488
GluI GCNGC 6 cut(s) 166, 169, 194, 435, 438, 488
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 207, 289
HapII CCGG 4 cut(s) 290, 326, 360, 447
Hin1II CATG 2 cut(s) 86, 431
HinfI GANTC 1 cut(s) 236
HpaII CCGG 4 cut(s) 290, 326, 360, 447
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 27, 67
Hpy188I TCNGA 8 cut(s) 34, 109, 235, 241, 253, 381, 404, 456
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 2 cut(s) 27, 67
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 2 cut(s) 63, 150
HpyCH4V TGCA 3 cut(s) 8, 95, 387
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 3 cut(s) 125, 209, 475
HpySE526I ACGT 2 cut(s) 63, 150
Hsp92II CATG 2 cut(s) 86, 431
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
LmnI GCTCC 5 cut(s) 180, 254, 292, 300, 404
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 17
MaeII ACGT 2 cut(s) 63, 150
MbiI CCGCTC 1 cut(s) 189
MboII GAAGA 3 cut(s) 145, 170, 406
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 3 cut(s) 368, 382, 499
MmeI TCCRAC 3 cut(s) 132, 213, 278
MnlI CCTC 8 cut(s) 40, 60, 68, 115, 130, 134, 338, 500
MroNI GCCGGC 1 cut(s) 446
MseI TTAA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 4 cut(s) 290, 326, 360, 447
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 443
NaeI GCCGGC 1 cut(s) 448
NcoI CCATGG 1 cut(s) 82
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 2 cut(s) 86, 431
NlaIV GGNNCC 2 cut(s) 250, 330
NspV TTCGAA 1 cut(s) 156
PagI TCATGA 1 cut(s) 427
PdiI GCCGGC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 6 cut(s) 167, 170, 195, 436, 439, 489
Ppu21I YACGTR 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 250, 330
PspPI GGNCC 3 cut(s) 205, 323, 452
PstNI CAGNNNCTG 1 cut(s) 202
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
Rsr2I CGGWCCG 2 cut(s) 323, 452
RsrII CGGWCCG 2 cut(s) 323, 452
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 6 cut(s) 166, 169, 194, 435, 438, 488
Sau96I GGNCC 3 cut(s) 205, 323, 452
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 409
SfaNI GCATC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 323, 452
Sse9I AATT 3 cut(s) 368, 382, 499
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 2 cut(s) 66, 153
TaqI TCGA 2 cut(s) 156, 273
TaqII GACCGA 1 cut(s) 311
TasI AATT 3 cut(s) 368, 382, 499
TauI GCSGC 5 cut(s) 168, 171, 196, 437, 490
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 26
TseI GCWGC 1 cut(s) 437
TspDTI ATGAA 2 cut(s) 406, 444
TspRI CASTG 1 cut(s) 26
VpaK11BI GGWCC 2 cut(s) 323, 452
XapI RAATTY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.