Rroxscaffold_1G00044460

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001151
Physical Location & Seq
Forward (+)
63429381 .. 63433155
3775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044460.1

Sequence Viewer

Length: 2433 bp
ATGAAGGGTCTTTTTATTATCTTCTTCACCTCTCTATTCTATTTTTCGGTCTTGAATCTGTCCACTGCAGCTGACACCCTAGCTGCATCGCAATCCATATTCGGCTCTGATACCTTGGTTTCTTCCAGCCAAAGCTTTGAACTGGGACTTTTTTCCACTGGCAACTCTAGAGCATGGTATCTAGGAATATGGTACAAGAATTTTCCAGATATTGTTGTATGGGTTGCAAACAGAGAAAATCCACTTGCAGATTCTTATGGAGCCATGATGTTGTCCAAAAATGGAAGTTTAGTCCTTTTAGACCAAATGAATAGAACTATTTGGTCTTCTAGTCCTTCCAAAGAAGCTGAAGATCCTGTTGCACAGCTTTTGGAAACTGGAAACCTTGTTGTAGTAGACAAGGCTGTGACAAGTTCCGAAAGCTATATATGGCAAAGTTTTGAATTCCCATCGGACACACTGCTACCAGGAATGAGGCTGCGATTGAACTTCAAAACAGGCCCAAACCAATTCTTGACATCTTGGAAAAATGCTAGTGACCCATCTCTTGGATTATATACCTATAAGATCGATCACCTTGTGTTGCCTCAGCTTGTTCTTGCTCAAGGATCAAAGAAAGAATTCCGCAGTGGACCTTGGAATGGTCTTCGATTTACTGGTCTTCCTGTTTCAAGCAACGAAATTTTGAAACCAAGTTATGTTTATAACACAAATGAGTTGTATTATATCTATGAGGCTAATGACAATTCTATTATTACCCGATTAAAGCTGACTGAAACAGGTGAGATGCAACGGCTAGTGCTGAATAAAGGAAGTACTGAATGGGGTGTTATGTATACTTTGCAGAATGATCGATGCGACAATTACGGGGAGTGTGGTGCAAATAGCATATGCAAAATTAACAGAACACCCATTTGTGAGTGCTTACAGGGGTTTGTTCCAAAATCACAGCAAGAATGGGAGGTTCTTAATAGGACAAGTGGTTGCAAGAGAGAGACCCTATTGGATTGCCAGAAGGGGGAAGGGTTTTTGAAATTTCAAAATATGAAACTTCCAGACCTGTTGGATTTTTCAGTGAACAACAGCATGAGCTTCAAGGAATGTGAGGCAGAGTGTTTGAAGAATTGTTCTTGTGTAGCTTATGCTAAATCAAATATGAGTAGTGGAGGCATTGGCTGTCTGATGTGGTTCGGGGAGCTTATAGATATGCGTGAGTTCGTTGATGAAGTTAGTGATCAAGATTTATATATTCGGATGCCAGCTTCAGAACTAGGCAATAGTACCAATCAGAAGGATAAAAGAGTAGTATTAATCTCAGTGATATCAGCAGCTTCTGTACTTCTTTTTCTGGGTCTCTTATGCCGTTGTATTGTTCTGAATAAGAGAGCAAAGAGAAAGGGTTCAAGATCAAGCAAGGAAGACATAGAGTTGCCACTATTTGATTTTCATACAATTGAAATCGGCACCAATTACTTTTCCTGGCAAAACAAACTTGGAGAGGGCGGTTTTGGTCCAGTTTACAAGGCTAACCTAAGACAAGAAGAATTAGTAGCAGTAAAGAGACTTTCAAGAGGCTCTGGACAAGGTCTTGAAGAGTTTAGGAATGAAGTTACAATGATAGCCAATCTCCAACACAGGAATCTTGTTAAACTTTTAGGCTGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCCAACAAAAGCTTGGACTTCTTTATCTTTGATCAAAACAGAAAGAAGCTGCTTAACTGGCAAAAGCGGTTCGACATTATCATGGGAATTGCACGAGGACTTCTCTACCTCCATCAAGACTCAAGATTAAGAATCATTCATAGGGACCTTAAGAGTAGCAACATCTTGCTAGACCGTGACCTGATCCCAAAAATATCCGACTTTGGCATAGCAAGAATTTTCGAAAGCAATCAAACTGAAGGAAAAACAAAACGAGTAATTGGCACATATGGATATATGTCTCCTGAGTACACAATTCATGGGAAATTTTCAGTGAAATCGGATGTGTTCAGTTTTGGAGTGCTATTATTAGAGATAGTTAGCAGCAGAAAAAACACAGGGTTCAATCATCCAGATCACAACCACAATCTTCTGGGACATGCATGGCTACTGTGGAACAACAACAAGGGTTTGGAACTGATAGATCCATGCTTGGAGTACTCATACGTGGACTACGAGGTGCTAAGATGCATTCAAGTGGGTCTATTATGTGTTCAAAAGCTACCCAATGACAGACCAGCAATGTCATCAGCGGTGTTCATGTTATGCAATGAAGGAGCAACATTGCCCGAACCAAAGGAGCCTGGTTTCTTCACAGAAAGAAGCTCCATAGACAACAAAACCTTAACAGGAGGAGCGAGAAGCCACACAGGACTCACACTTACTATTTCGACATTGGAAGCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

810

Amino Acids

91.57

Weight (kDa)

6.84

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 176 4.1e-35 D-mannose binding lectin
S_locus_glycop PF00954 208 - 316 9.2e-28 S-locus glycoprotein domain
PAN_2 PF08276 337 - 403 1.4e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 494 - 694 1.6e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 495 - 694 6e-44 Protein kinase domain
DUF3403 PF11883 765 - 810 1.3e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 1463
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 2 cut(s) 396, 836
AciI CCGC 4 cut(s) 625, 1503, 1771, 2276
AclWI GGATC 4 cut(s) 347, 616, 1882, 2162
AcsI RAATTY 7 cut(s) 199, 443, 620, 681, 1034, 1920, 2009
AcuI CTGAAG 3 cut(s) 369, 1248, 1962
AdeI CACNNNGTG 1 cut(s) 580
AfaI GTAC 7 cut(s) 194, 817, 1282, 1338, 1700, 1994, 2183
AfiI CCNNNNNNNGG 3 cut(s) 548, 641, 1018
AflII CTTAAG 1 cut(s) 1853
AjnI CCWGG 3 cut(s) 466, 1478, 2326
Alw26I GTCTC 4 cut(s) 989, 1358, 1555, 1989
AlwI GGATC 4 cut(s) 347, 616, 1882, 2162
AlwNI CAGNNNCTG 1 cut(s) 1334
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 8 cut(s) 68, 83, 478, 1328, 1659, 1662, 1753, 2067
ApoI RAATTY 7 cut(s) 199, 443, 620, 681, 1034, 1920, 2009
AseI ATTAAT 1 cut(s) 1310
Asp700I GAANNNNTTC 2 cut(s) 620, 1399
AspS9I GGNCC 4 cut(s) 500, 632, 1511, 1849
AsuHPI GGTGA 3 cut(s) 19, 566, 794
AsuII TTCGAA 1 cut(s) 1926
AvaII GGWCC 3 cut(s) 632, 1511, 1849
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BanI GGYRCC 1 cut(s) 1463
BauI CACGAG 1 cut(s) 1797
BbsI GAAGAC 4 cut(s) 318, 638, 653, 1425
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 8 cut(s) 70, 80, 465, 1340, 1646, 1649, 1740, 2079
BccI CCATC 3 cut(s) 457, 550, 1824
BceAI ACGGC 2 cut(s) 809, 1347
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 3 cut(s) 468, 1480, 2328
BclI TGATCA 2 cut(s) 1234, 1735
BcoDI GTCTC 4 cut(s) 989, 1358, 1555, 1989
BfaI CTAG 9 cut(s) 80, 168, 182, 330, 534, 797, 1271, 1874, 2427
BfmI CTRYAG 1 cut(s) 66
BfrI CTTAAG 1 cut(s) 1853
BisI GCNGC 8 cut(s) 69, 84, 479, 1329, 1660, 1663, 1754, 2068
BlsI GCNGC 8 cut(s) 70, 85, 480, 1330, 1661, 1664, 1755, 2069
BmcAI AGTACT 2 cut(s) 817, 2183
Bme1390I CCNGG 3 cut(s) 468, 1480, 2328
Bme18I GGWCC 3 cut(s) 632, 1511, 1849
BmgT120I GGNCC 4 cut(s) 500, 632, 1511, 1849
BmiI GGNNCC 4 cut(s) 262, 1465, 1850, 2325
BmrFI CCNGG 3 cut(s) 468, 1480, 2328
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 6 cut(s) 95, 777, 845, 1245, 1674, 2201
BmuI ACTGGG 1 cut(s) 152
BpiI GAAGAC 4 cut(s) 318, 638, 653, 1425
BplI GAGNNNNNCTC 2 cut(s) 1791, 1823
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 1926
BpuEI CTTGAG 2 cut(s) 588, 1810
Bsa29I ATCGAT 2 cut(s) 570, 853
BsaAI YACGTR 1 cut(s) 2191
BsaBI GATNNNNATC 1 cut(s) 1688
BsaI GGTCTC 2 cut(s) 989, 1358
BsaJI CCNNGG 2 cut(s) 114, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 548, 641, 1018
Bse1I ACTGG 6 cut(s) 147, 163, 382, 661, 1514, 1766
Bse3DI GCAATG 3 cut(s) 2271, 2299, 2306
Bse8I GATNNNNATC 1 cut(s) 1688
BseBI CCWGG 3 cut(s) 468, 1480, 2328
BseCI ATCGAT 2 cut(s) 570, 853
BseDI CCNNGG 2 cut(s) 114, 635
BseGI GGATG 4 cut(s) 1260, 1689, 2032, 2092
BseJI GATNNNNATC 1 cut(s) 1688
BseLI CCNNNNNNNGG 3 cut(s) 548, 641, 1018
BseMI GCAATG 3 cut(s) 2271, 2299, 2306
BseMII CTCAG 3 cut(s) 602, 1329, 1980
BseNI ACTGG 6 cut(s) 147, 163, 382, 661, 1514, 1766
BseRI GAGGAG 1 cut(s) 2391
BseXI GCAGC 8 cut(s) 70, 80, 465, 1340, 1646, 1649, 1740, 2079
BshFI GGCC 1 cut(s) 501
BshNI GGYRCC 1 cut(s) 1463
BshVI ATCGAT 2 cut(s) 570, 853
BslFI GGGAC 3 cut(s) 159, 1862, 2133
BslI CCNNNNNNNGG 3 cut(s) 548, 641, 1018
BsmAI GTCTC 4 cut(s) 989, 1358, 1555, 1989
BsmFI GGGAC 3 cut(s) 159, 1862, 2133
BsmI GAATGC 1 cut(s) 2214
BsnI GGCC 1 cut(s) 501
Bso31I GGTCTC 2 cut(s) 989, 1358
Bsp119I TTCGAA 1 cut(s) 1926
BspACI CCGC 4 cut(s) 625, 1503, 1771, 2276
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 3 cut(s) 601, 1328, 1981
BspDI ATCGAT 2 cut(s) 570, 853
BspLI GGNNCC 4 cut(s) 262, 1465, 1850, 2325
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 4 cut(s) 347, 616, 1882, 2162
BspT104I TTCGAA 1 cut(s) 1926
BspT107I GGYRCC 1 cut(s) 1463
BspTI CTTAAG 1 cut(s) 1853
BspTNI GGTCTC 2 cut(s) 989, 1358
BsrDI GCAATG 3 cut(s) 2271, 2299, 2306
BsrI ACTGG 6 cut(s) 147, 163, 382, 661, 1514, 1766
BssECI CCNNGG 2 cut(s) 114, 635
BssNAI GTATAC 1 cut(s) 837
BssSI CACGAG 1 cut(s) 1797
BssT1I CCWWGG 2 cut(s) 114, 635
Bst1107I GTATAC 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 1797
Bst2UI CCWGG 3 cut(s) 468, 1480, 2328
Bst4CI ACNGT 2 cut(s) 1880, 2136
Bst6I CTCTTC 1 cut(s) 1587
BstAFI CTTAAG 1 cut(s) 1853
BstBAI YACGTR 1 cut(s) 2191
BstBI TTCGAA 1 cut(s) 1926
BstC8I GCNNGC 1 cut(s) 1260
BstDEI CTNAG 5 cut(s) 588, 1315, 1532, 1989, 2207
BstF5I GGATG 4 cut(s) 1260, 1689, 2032, 2092
BstMAI GTCTC 4 cut(s) 989, 1358, 1555, 1989
BstMWI GCNNNNNNNGC 1 cut(s) 1762
BstNI CCWGG 3 cut(s) 468, 1480, 2328
BstNSI RCATGY 2 cut(s) 1705, 2126
BstSCI CCNGG 3 cut(s) 466, 1478, 2326
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 8 cut(s) 70, 80, 465, 1340, 1646, 1649, 1740, 2079
BstV2I GAAGAC 4 cut(s) 318, 638, 653, 1425
BstX2I RGATCY 2 cut(s) 352, 2167
BstYI RGATCY 2 cut(s) 352, 2167
BstZ17I GTATAC 1 cut(s) 837
Bsu15I ATCGAT 2 cut(s) 570, 853
BsuRI GGCC 1 cut(s) 501
BsuTUI ATCGAT 2 cut(s) 570, 853
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 4 cut(s) 1260, 1689, 2032, 2092
BtsI GCAGTG 3 cut(s) 63, 458, 634
BtsIMutI CAGTG 7 cut(s) 63, 156, 458, 634, 1080, 1323, 2022
Cac8I GCNNGC 1 cut(s) 1260
CaiI CAGNNNCTG 1 cut(s) 1334
Cfr13I GGNCC 4 cut(s) 500, 632, 1511, 1849
ClaI ATCGAT 2 cut(s) 570, 853
Csp6I GTAC 7 cut(s) 193, 816, 1281, 1337, 1699, 1993, 2182
CviQI GTAC 7 cut(s) 193, 816, 1281, 1337, 1699, 1993, 2182
DdeI CTNAG 5 cut(s) 588, 1315, 1532, 1989, 2207
DraIII CACNNNGTG 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 1587
EarI CTCTTC 1 cut(s) 1587
Eco130I CCWWGG 2 cut(s) 114, 635
Eco31I GGTCTC 2 cut(s) 989, 1358
Eco32I GATATC 1 cut(s) 1323
Eco47I GGWCC 3 cut(s) 632, 1511, 1849
Eco57I CTGAAG 3 cut(s) 369, 1248, 1962
EcoO109I RGGNCCY 1 cut(s) 1849
EcoRI GAATTC 2 cut(s) 443, 620
EcoRII CCWGG 3 cut(s) 466, 1478, 2326
EcoRV GATATC 1 cut(s) 1323
EcoT14I CCWWGG 2 cut(s) 114, 635
EcoT22I ATGCAT 2 cut(s) 2128, 2216
ErhI CCWWGG 2 cut(s) 114, 635
FaqI GGGAC 3 cut(s) 159, 1862, 2133
FauNDI CATATG 2 cut(s) 890, 1972
FbaI TGATCA 2 cut(s) 1234, 1735
FblI GTMKAC 2 cut(s) 396, 836
Fnu4HI GCNGC 8 cut(s) 69, 84, 479, 1329, 1660, 1663, 1754, 2068
FokI GGATG 4 cut(s) 1267, 1696, 2039, 2079
Fsp4HI GCNGC 8 cut(s) 69, 84, 479, 1329, 1660, 1663, 1754, 2068
FspBI CTAG 9 cut(s) 80, 168, 182, 330, 534, 797, 1271, 1874, 2427
GluI GCNGC 8 cut(s) 69, 84, 479, 1329, 1660, 1663, 1754, 2068
HaeIII GGCC 1 cut(s) 501
HindIII AAGCTT 2 cut(s) 133, 1714
HinfI GANTC 6 cut(s) 55, 251, 1639, 1823, 1836, 2397
HphI GGTGA 3 cut(s) 19, 566, 794
Hpy166II GTNNAC 8 cut(s) 63, 397, 632, 837, 1078, 1519, 1995, 2194
Hpy8I GTNNAC 8 cut(s) 63, 397, 632, 837, 1078, 1519, 1995, 2194
HpyAV CCTTC 7 cut(s) 345, 1009, 1016, 1285, 1664, 1937, 2291
HpyCH4III ACNGT 2 cut(s) 1880, 2136
HpyCH4IV ACGT 1 cut(s) 2190
HpyF10VI GCNNNNNNNGC 1 cut(s) 1762
HpyF3I CTNAG 5 cut(s) 588, 1315, 1532, 1989, 2207
HpySE526I ACGT 1 cut(s) 2190
Ksp22I TGATCA 2 cut(s) 1234, 1735
LmnI GCTCC 6 cut(s) 260, 1195, 2300, 2323, 2354, 2378
Lsp1109I GCAGC 8 cut(s) 70, 80, 465, 1340, 1646, 1649, 1740, 2079
LweI GCATC 6 cut(s) 95, 777, 845, 1245, 1674, 2201
MaeI CTAG 9 cut(s) 80, 168, 182, 330, 534, 797, 1271, 1874, 2427
MaeII ACGT 1 cut(s) 2190
MaeIII GTNAC 4 cut(s) 406, 536, 1609, 1880
MfeI CAATTG 1 cut(s) 1452
MflI RGATCY 2 cut(s) 352, 2167
MlyI GAGTC 2 cut(s) 1817, 2391
MmeI TCCRAC 3 cut(s) 1044, 1654, 1926
Mph1103I ATGCAT 2 cut(s) 2128, 2216
MroXI GAANNNNTTC 2 cut(s) 620, 1399
MseI TTAA 9 cut(s) 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369
MslI CAYNNNNRTG 2 cut(s) 1784, 2219
MspA1I CMGCKG 2 cut(s) 71, 2276
MspCI CTTAAG 1 cut(s) 1853
MspR9I CCNGG 3 cut(s) 468, 1480, 2328
MunI CAATTG 1 cut(s) 1452
Mva1269I GAATGC 1 cut(s) 2214
MvaI CCWGG 3 cut(s) 468, 1480, 2328
MwoI GCNNNNNNNGC 1 cut(s) 1762
NdeI CATATG 2 cut(s) 890, 1972
NlaIV GGNNCC 4 cut(s) 262, 1465, 1850, 2325
NmuCI GTSAC 3 cut(s) 406, 536, 1880
NsiI ATGCAT 2 cut(s) 2128, 2216
NspI RCATGY 2 cut(s) 1705, 2126
NspV TTCGAA 1 cut(s) 1926
PcsI WCGNNNNNNNCGW 1 cut(s) 2196
PctI GAATGC 1 cut(s) 2214
PdmI GAANNNNTTC 2 cut(s) 620, 1399
PfeI GAWTC 4 cut(s) 55, 251, 1639, 1836
PflFI GACNNNGTC 1 cut(s) 1584
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 8 cut(s) 70, 85, 480, 1330, 1661, 1664, 1755, 2069
PleI GAGTC 2 cut(s) 1817, 2391
PpsI GAGTC 2 cut(s) 1817, 2391
Ppu21I YACGTR 1 cut(s) 2191
PpuMI RGGWCCY 1 cut(s) 1849
PshBI ATTAAT 1 cut(s) 1310
PsiI TTATAA 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 1849
Psp6I CCWGG 3 cut(s) 466, 1478, 2326
PspGI CCWGG 3 cut(s) 466, 1478, 2326
PspN4I GGNNCC 4 cut(s) 262, 1465, 1850, 2325
PspPI GGNCC 4 cut(s) 500, 632, 1511, 1849
PspPPI RGGWCCY 1 cut(s) 1849
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 1334
PsuI RGATCY 2 cut(s) 352, 2167
PsyI GACNNNGTC 1 cut(s) 1584
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 194, 817, 1282, 1338, 1700, 1994, 2183
RsaNI GTAC 7 cut(s) 193, 816, 1281, 1337, 1699, 1993, 2182
RseI CAYNNNNRTG 2 cut(s) 1784, 2219
SaqAI TTAA 9 cut(s) 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369
SatI GCNGC 8 cut(s) 69, 84, 479, 1329, 1660, 1663, 1754, 2068
Sau96I GGNCC 4 cut(s) 500, 632, 1511, 1849
ScaI AGTACT 2 cut(s) 817, 2183
SchI GAGTC 2 cut(s) 1817, 2391
ScrFI CCNGG 3 cut(s) 468, 1480, 2328
SfaNI GCATC 6 cut(s) 95, 777, 845, 1245, 1674, 2201
SfcI CTRYAG 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 1926
SinI GGWCC 3 cut(s) 632, 1511, 1849
SmiMI CAYNNNNRTG 2 cut(s) 1784, 2219
SmlI CTYRAG 3 cut(s) 603, 1825, 1853
SmoI CTYRAG 3 cut(s) 603, 1825, 1853
SsiI CCGC 4 cut(s) 625, 1503, 1771, 2276
SspMI CTAG 9 cut(s) 80, 168, 182, 330, 534, 797, 1271, 1874, 2427
StyD4I CCNGG 3 cut(s) 466, 1478, 2326
StyI CCWWGG 2 cut(s) 114, 635
TaaI ACNGT 2 cut(s) 1880, 2136
TaiI ACGT 1 cut(s) 2193
TaqI TCGA 6 cut(s) 570, 649, 853, 1776, 1926, 2414
TaqII GACCGA 1 cut(s) 37
TatI WGTACW 5 cut(s) 815, 1336, 1698, 1992, 2181
TfiI GAWTC 4 cut(s) 55, 251, 1639, 1836
Tru1I TTAA 9 cut(s) 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369
Tru9I TTAA 9 cut(s) 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369
TscAI CASTG 7 cut(s) 70, 163, 465, 634, 1080, 1323, 2022
TseFI GTSAC 3 cut(s) 406, 536, 1880
TseI GCWGC 8 cut(s) 68, 83, 478, 1328, 1659, 1662, 1753, 2067
Tsp45I GTSAC 3 cut(s) 406, 536, 1880
TspRI CASTG 7 cut(s) 70, 163, 465, 634, 1080, 1323, 2022
Tth111I GACNNNGTC 1 cut(s) 1584
Van91I CCANNNNNTGG 1 cut(s) 548
Vha464I CTTAAG 1 cut(s) 1853
VpaK11BI GGWCC 3 cut(s) 632, 1511, 1849
VspI ATTAAT 1 cut(s) 1310
XapI RAATTY 7 cut(s) 199, 443, 620, 681, 1034, 1920, 2009
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 2 cut(s) 1705, 2126
XcmI CCANNNNNNNNNTGG 1 cut(s) 1714
XmiI GTMKAC 2 cut(s) 396, 836
XmnI GAANNNNTTC 2 cut(s) 620, 1399
XspI CTAG 9 cut(s) 80, 168, 182, 330, 534, 797, 1271, 1874, 2427
ZrmI AGTACT 2 cut(s) 817, 2183
Zsp2I ATGCAT 2 cut(s) 2128, 2216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.