MD03G1186100.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001151
Physical Location & Seq
Reverse (-)
25354730 .. 25358156
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1186100.v1.1.491

Sequence Viewer

Length: 1914 bp
ATGATTTCGTTACCAGATGATCCATGTGACAGTTATGGATACTGTGGTGCTAATACTATTTGTAGAAACAGGTATCCCATATGCGTGTGTTTGCAAGGGTTCACTCCAAGGTTTCAAGAAGAATGGGAAATGTTTAATAGAACCAGTGGATGCTTGAGATGGACACCGTTGGATTGCCACAGCAGAGAGGGATTTTTACTACTTGAAGGGATGAAATTGCCTGGCATGTTAGAATTTTCACTCAATGAGAGTATGAATCTCGAGGAATGTATGGAGAAGTGCTTAAAAAATTGTTTTATAATTTTATCGATATTACTCCATTTTCTACTATTACAATTCTCCACAGCAGCTGATACCATTTCTCCATCAGAATCCATCACCGGCACGAATACCTTGGTTTCTTCCGGCACAAGCTTCCAATTAGGCTTCTTCTCTGCTGGCAGTTTACGGTATTTAGGAATATGGTACAAGAACTTTCCAGATACTGTTGTATGGGTGGCCAACAGAGAAAAGCCTCTCGCAGATTTAAATGGATCCTTGACATTATCGAAAAACGGAAGCCTGATCCTCTTAGACAACATGAACAACACTCTTTGGTCGTCCAGTTCTTCCCGAGCAGCAGAAGATCCTGTTGCGCAGCTTTTGGAGACTGGAAATCTTGTTGTTAGAGACAAGGCCGCAACTGATTCAGAAGATTATGTATGGCAAAGTTTCGATTTCCCTACGGACACTTTGTTGCAGGAAATGAAGTTGGGGTGGAACTTCGGAACCGGCCTCAACAGATTCTTGACTTCTTGGAAAAATGCTAGCGACCCTTCTCCTGGAGAATATACTTACGGGATGGATCGTGTTTTGTTGCCTCAGCTTGTTATTTCAAAAGGATCGAAGAAAAAGTTTCGTACTGGACCTTGGAACGGTATCCGAATCACAGGTACTCCTGGTGCAGCCCTTTCGTTCGGAGCGGTTGTGAATCCAATTTTTGTGTATAATTCCAAAGAGTTATACTATGCATATGGGGCTGTCAGTAACTCTACCATCACAAGGTTAAAGCTTACTGAATCGGGCGAGATCCAGCGGTTACTTGTCAACAATGGAAGCACTGAATGGGCTGTTATGTATACTTTGCGGAATGATGAGTGCAGTAATTATGGACAGTGTGGAGCAAATGGCTTTTGCAAGATTAACAAATCACCCATTTGTGGCTGCTTGCCGGGGTTTTTTCCGACATCAACGAGTGAATGGGGGGTGCTTAATTGGACTCGTGGGTGCAGGAGGGAGACCCCACTGAATTGTTCGAAGGGAGAAGGATTTCTGAAAATCGAAAATGTGAAACTTCCGGACCAATTGGATTTTTGGGTCAAGAATAGCACTAGCACCAGCGAATGCAAGGCAGAGTGCTTGAAGAATTGTTCTTGTGTAGCTTATGCTAACTCAGATATCAAAAATGAAAGCAGTCTCTGTCTGATGTGGTTCGGCGACCTAATTGATCTGCGCGAGTTCGTTGAAGAAGATATTGATCAAGAGCAAGATATATATATTCGGGTGCCATGGAAAACGAGTGGGAAGAATGATAAAAAAGTAGTACTCATTTCAGTCATATCAGCGGTTTGTTTGGTACTTTTTCTCGGTTTGGCTTTGTTTTCCTGGTGTATAATTCTGAAGAGGAGAAGAAATAAAAGAGCTTCAAGAGACGGTGAGGAGAACTTGGATTTGCCACTGTTTGATTTTGATACAATTGCAGCCGCCACCAATCACTTCTCCCACACAAACAAGCTAGGAGAGGGAGGTTTTGGTTCGGTTTACAAGGCCAACTTAACCCAAGAAGAATTCATTGCTGTAAAGAGACTCTCAAAAGATTCTGGCCAAGGAATTGAAGAGTTTAAGAACGAAGTTGCGATGATAGCCAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

638

Amino Acids

71.21

Weight (kDa)

5.37

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 2 - 37 1.5e-06 S-locus glycoprotein domain
PAN_2 PF08276 59 - 98 4.1e-06 PAN-like domain
B_lectin PF01453 162 - 267 6.2e-37 D-mannose binding lectin
S_locus_glycop PF00954 299 - 409 3.8e-23 S-locus glycoprotein domain
PAN_2 PF08276 431 - 497 1.6e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 299
Acc16I TGCGCA 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 1543
AccBSI CCGCTC 1 cut(s) 962
AccI GTMKAC 1 cut(s) 1118
AccII CGCG 1 cut(s) 1494
AccIII TCCGGA 1 cut(s) 1336
AciI CCGC 6 cut(s) 678, 962, 1075, 1126, 1604, 1743
AclWI GGATC 8 cut(s) 14, 528, 541, 559, 620, 852, 889, 1063
AcoI YGGCCR 2 cut(s) 498, 1861
AcsI RAATTY 2 cut(s) 233, 1826
AcuI CTGAAG 1 cut(s) 1679
AfaI GTAC 5 cut(s) 467, 901, 934, 1584, 1617
AfiI CCNNNNNNNGG 4 cut(s) 821, 914, 1041, 1199
AgsI TTSAA 7 cut(s) 116, 206, 876, 1402, 1505, 1686, 1874
AjnI CCWGG 4 cut(s) 220, 820, 937, 1643
AluBI AGCT 8 cut(s) 350, 414, 640, 865, 1051, 1421, 1682, 1774
AluI AGCT 8 cut(s) 350, 414, 640, 865, 1051, 1421, 1682, 1774
Alw26I GTCTC 6 cut(s) 641, 663, 1271, 1460, 1683, 1837
AlwI GGATC 8 cut(s) 14, 528, 541, 559, 620, 852, 889, 1063
AlwNI CAGNNNCTG 3 cut(s) 350, 485, 1458
Ama87I CYCGRG 2 cut(s) 260, 612
Aor13HI TCCGGA 1 cut(s) 1336
AoxI GGCC 5 cut(s) 498, 675, 772, 1806, 1861
ApeKI GCWGC 6 cut(s) 347, 617, 637, 944, 1203, 1739
ApoI RAATTY 2 cut(s) 233, 1826
Asp700I GAANNNNTTC 1 cut(s) 1308
AspLEI GCGC 2 cut(s) 637, 1494
AspS9I GGNCC 2 cut(s) 905, 1339
AsuC2I CCSGG 1 cut(s) 1212
AsuHPI GGTGA 3 cut(s) 370, 1182, 1706
AsuII TTCGAA 1 cut(s) 1295
AsuNHI GCTAGC 1 cut(s) 806
AvaI CYCGRG 2 cut(s) 260, 612
AvaII GGWCC 2 cut(s) 905, 1339
BaeI ACNNNNGTAYC 4 cut(s) 31, 64, 474, 507
BalI TGGCCA 2 cut(s) 500, 1863
BamHI GGATCC 1 cut(s) 533
BanI GGYRCC 1 cut(s) 1543
BauI CACGAG 1 cut(s) 1260
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 6 cut(s) 359, 629, 649, 956, 1190, 1751
BccI CCATC 5 cut(s) 153, 373, 383, 835, 1043
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BciT130I CCWGG 4 cut(s) 222, 822, 939, 1645
BciVI GTATCC 3 cut(s) 32, 84, 929
BclI TGATCA 1 cut(s) 1516
BcnI CCSGG 1 cut(s) 1212
BcoDI GTCTC 6 cut(s) 641, 663, 1271, 1460, 1683, 1837
BfaI CTAG 3 cut(s) 807, 1371, 1775
BfuI GTATCC 3 cut(s) 32, 84, 929
BisI GCNGC 8 cut(s) 348, 618, 638, 678, 945, 1204, 1740, 1743
BlsI GCNGC 8 cut(s) 349, 619, 639, 679, 946, 1205, 1741, 1744
BmcAI AGTACT 1 cut(s) 1584
Bme1390I CCNGG 5 cut(s) 222, 822, 939, 1212, 1645
Bme18I GGWCC 2 cut(s) 905, 1339
BmeT110I CYCGRG 2 cut(s) 260, 612
BmgT120I GGNCC 2 cut(s) 905, 1339
BmiI GGNNCC 3 cut(s) 535, 769, 1545
BmrFI CCNGG 5 cut(s) 222, 822, 939, 1212, 1645
BmsI GCATC 1 cut(s) 140
BmtI GCTAGC 1 cut(s) 810
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 1295
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 1212
Bsa29I ATCGAT 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 1515
BsaI GGTCTC 1 cut(s) 1271
BsaJI CCNNGG 6 cut(s) 107, 393, 908, 1211, 1547, 1864
BsaWI WCCGGW 1 cut(s) 1336
BsaXI ACNNNNNCTCC 6 cut(s) 346, 376, 919, 949, 951, 981
Bsc4I CCNNNNNNNGG 4 cut(s) 821, 914, 1041, 1199
Bse118I RCCGGY 2 cut(s) 380, 770
Bse1I ACTGG 4 cut(s) 144, 603, 655, 907
Bse3DI GCAATG 1 cut(s) 1830
Bse8I GATNNNNATC 1 cut(s) 1515
BseAI TCCGGA 1 cut(s) 1336
BseBI CCWGG 4 cut(s) 222, 822, 939, 1645
BseCI ATCGAT 1 cut(s) 308
BseDI CCNNGG 6 cut(s) 107, 393, 908, 1211, 1547, 1864
BseGI GGATG 3 cut(s) 155, 216, 846
BseJI GATNNNNATC 1 cut(s) 1515
BseLI CCNNNNNNNGG 4 cut(s) 821, 914, 1041, 1199
BseMI GCAATG 1 cut(s) 1830
BseMII CTCAG 2 cut(s) 875, 1446
BseNI ACTGG 4 cut(s) 144, 603, 655, 907
BseRI GAGGAG 2 cut(s) 1678, 1712
BseXI GCAGC 6 cut(s) 359, 629, 649, 956, 1190, 1751
BsgI GTGCAG 3 cut(s) 963, 1159, 1288
Bsh1236I CGCG 1 cut(s) 1494
BshFI GGCC 5 cut(s) 500, 677, 774, 1808, 1863
BshNI GGYRCC 1 cut(s) 1543
BshVI ATCGAT 1 cut(s) 308
BsiHKCI CYCGRG 2 cut(s) 260, 612
BsiSI CCGG 5 cut(s) 381, 405, 771, 1211, 1337
BslI CCNNNNNNNGG 4 cut(s) 821, 914, 1041, 1199
BsmAI GTCTC 6 cut(s) 641, 663, 1271, 1460, 1683, 1837
BsmBI CGTCTC 1 cut(s) 1683
BsmI GAATGC 1 cut(s) 1388
BsnI GGCC 5 cut(s) 500, 677, 774, 1808, 1863
Bso31I GGTCTC 1 cut(s) 1271
BsoBI CYCGRG 2 cut(s) 260, 612
Bsp119I TTCGAA 1 cut(s) 1295
Bsp13I TCCGGA 1 cut(s) 1336
Bsp143I GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
Bsp19I CCATGG 1 cut(s) 1547
BspACI CCGC 6 cut(s) 678, 962, 1075, 1126, 1604, 1743
BspANI GGCC 5 cut(s) 500, 677, 774, 1808, 1863
BspCNI CTCAG 2 cut(s) 874, 1445
BspDI ATCGAT 1 cut(s) 308
BspEI TCCGGA 1 cut(s) 1336
BspFNI CGCG 1 cut(s) 1494
BspLI GGNNCC 3 cut(s) 535, 769, 1545
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 8 cut(s) 14, 528, 541, 559, 620, 852, 889, 1063
BspT104I TTCGAA 1 cut(s) 1295
BspT107I GGYRCC 1 cut(s) 1543
BspTNI GGTCTC 1 cut(s) 1271
BsrBI CCGCTC 1 cut(s) 962
BsrDI GCAATG 1 cut(s) 1830
BsrFI RCCGGY 2 cut(s) 380, 770
BsrI ACTGG 4 cut(s) 144, 603, 655, 907
BssAI RCCGGY 2 cut(s) 380, 770
BssECI CCNNGG 6 cut(s) 107, 393, 908, 1211, 1547, 1864
BssMI GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
BssNAI GTATAC 1 cut(s) 1119
BssSI CACGAG 1 cut(s) 1260
BssT1I CCWWGG 5 cut(s) 107, 393, 908, 1547, 1864
Bst1107I GTATAC 1 cut(s) 1119
Bst2BI CACGAG 1 cut(s) 1260
Bst2UI CCWGG 4 cut(s) 222, 822, 939, 1645
Bst4CI ACNGT 9 cut(s) 32, 44, 168, 450, 487, 917, 1155, 1694, 1719
Bst6I CTCTTC 2 cut(s) 1655, 1869
BstBI TTCGAA 1 cut(s) 1295
BstC8I GCNNGC 3 cut(s) 439, 808, 1208
BstDEI CTNAG 3 cut(s) 571, 861, 1432
BstDSI CCRYGG 1 cut(s) 1547
BstF5I GGATG 3 cut(s) 155, 216, 846
BstFNI CGCG 1 cut(s) 1494
BstHHI GCGC 2 cut(s) 637, 1494
BstKTI GATC 9 cut(s) 22, 536, 567, 628, 847, 884, 1071, 1489, 1519
BstMAI GTCTC 6 cut(s) 641, 663, 1271, 1460, 1683, 1837
BstMBI GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
BstMWI GCNNNNNNNGC 2 cut(s) 1016, 1901
BstNI CCWGG 4 cut(s) 222, 822, 939, 1645
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 5 cut(s) 220, 820, 937, 1210, 1643
BstUI CGCG 1 cut(s) 1494
BstV1I GCAGC 6 cut(s) 359, 629, 649, 956, 1190, 1751
BstX2I RGATCY 3 cut(s) 533, 625, 1068
BstYI RGATCY 3 cut(s) 533, 625, 1068
BstZ17I GTATAC 1 cut(s) 1119
Bsu15I ATCGAT 1 cut(s) 308
BsuI GTATCC 3 cut(s) 32, 84, 929
BsuRI GGCC 5 cut(s) 500, 677, 774, 1808, 1863
BsuTUI ATCGAT 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 1547
BtgZI GCGATG 1 cut(s) 1910
BtsCI GGATG 3 cut(s) 155, 216, 846
BtsIMutI CAGTG 5 cut(s) 151, 1098, 1160, 1283, 1715
Cac8I GCNNGC 3 cut(s) 439, 808, 1208
CaiI CAGNNNCTG 3 cut(s) 350, 485, 1458
CfoI GCGC 2 cut(s) 637, 1494
Cfr10I RCCGGY 2 cut(s) 380, 770
Cfr13I GGNCC 2 cut(s) 905, 1339
ClaI ATCGAT 1 cut(s) 308
Csp6I GTAC 5 cut(s) 466, 900, 933, 1583, 1616
CviAII CATG 4 cut(s) 24, 226, 580, 1548
CviQI GTAC 5 cut(s) 466, 900, 933, 1583, 1616
DdeI CTNAG 3 cut(s) 571, 861, 1432
DpnI GATC 9 cut(s) 21, 535, 566, 627, 846, 883, 1070, 1488, 1518
DpnII GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
DraI TTTAAA 1 cut(s) 528
EaeI YGGCCR 2 cut(s) 498, 1861
Eam1104I CTCTTC 2 cut(s) 1655, 1869
EarI CTCTTC 2 cut(s) 1655, 1869
Eco130I CCWWGG 5 cut(s) 107, 393, 908, 1547, 1864
Eco31I GGTCTC 1 cut(s) 1271
Eco32I GATATC 1 cut(s) 1438
Eco47I GGWCC 2 cut(s) 905, 1339
Eco57I CTGAAG 1 cut(s) 1679
Eco88I CYCGRG 2 cut(s) 260, 612
EcoRI GAATTC 1 cut(s) 1826
EcoRII CCWGG 4 cut(s) 220, 820, 937, 1643
EcoRV GATATC 1 cut(s) 1438
EcoT14I CCWWGG 5 cut(s) 107, 393, 908, 1547, 1864
EcoT22I ATGCAT 1 cut(s) 1012
ErhI CCWWGG 5 cut(s) 107, 393, 908, 1547, 1864
Esp3I CGTCTC 1 cut(s) 1683
FaeI CATG 4 cut(s) 27, 229, 583, 1551
FalI AAGNNNNNCTT 2 cut(s) 1796, 1828
FatI CATG 4 cut(s) 23, 225, 579, 1547
FauNDI CATATG 2 cut(s) 80, 1012
FbaI TGATCA 1 cut(s) 1516
FblI GTMKAC 1 cut(s) 1118
Fnu4HI GCNGC 8 cut(s) 348, 618, 638, 678, 945, 1204, 1740, 1743
FokI GGATG 3 cut(s) 162, 223, 853
Fsp4HI GCNGC 8 cut(s) 348, 618, 638, 678, 945, 1204, 1740, 1743
FspBI CTAG 3 cut(s) 807, 1371, 1775
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 2 cut(s) 636, 1493
GluI GCNGC 8 cut(s) 348, 618, 638, 678, 945, 1204, 1740, 1743
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 5 cut(s) 500, 677, 774, 1808, 1863
HapII CCGG 5 cut(s) 381, 405, 771, 1211, 1337
HhaI GCGC 2 cut(s) 637, 1494
Hin1II CATG 4 cut(s) 27, 229, 583, 1551
Hin6I GCGC 2 cut(s) 635, 1492
HinP1I GCGC 2 cut(s) 635, 1492
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 2 cut(s) 412, 1049
HpaII CCGG 5 cut(s) 381, 405, 771, 1211, 1337
HphI GGTGA 3 cut(s) 370, 1182, 1706
Hpy166II GTNNAC 5 cut(s) 102, 446, 1087, 1119, 1801
Hpy188III TCNNGA 9 cut(s) 116, 260, 479, 612, 787, 1337, 1360, 1520, 1686
Hpy8I GTNNAC 5 cut(s) 102, 446, 1087, 1119, 1801
HpyAV CCTTC 4 cut(s) 200, 825, 1291, 1298
HpyCH4III ACNGT 9 cut(s) 32, 44, 168, 450, 487, 917, 1155, 1694, 1719
HpyCH4V TGCA 9 cut(s) 94, 739, 944, 1010, 1140, 1176, 1269, 1386, 1739
HpyF10VI GCNNNNNNNGC 2 cut(s) 1016, 1901
HpyF3I CTNAG 3 cut(s) 571, 861, 1432
Hsp92II CATG 4 cut(s) 27, 229, 583, 1551
HspAI GCGC 2 cut(s) 635, 1492
Kpn2I TCCGGA 1 cut(s) 1336
Ksp22I TGATCA 1 cut(s) 1516
Kzo9I GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
LmnI GCTCC 2 cut(s) 959, 1160
Lsp1109I GCAGC 6 cut(s) 359, 629, 649, 956, 1190, 1751
LweI GCATC 1 cut(s) 140
MaeI CTAG 3 cut(s) 807, 1371, 1775
MaeIII GTNAC 4 cut(s) 9, 26, 1025, 1077
MalI GATC 9 cut(s) 21, 535, 566, 627, 846, 883, 1070, 1488, 1518
MbiI CCGCTC 1 cut(s) 962
MboI GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
MfeI CAATTG 2 cut(s) 1343, 1734
MflI RGATCY 3 cut(s) 533, 625, 1068
MlsI TGGCCA 2 cut(s) 500, 1863
MluNI TGGCCA 2 cut(s) 500, 1863
MlyI GAGTC 2 cut(s) 1252, 1839
MmeI TCCRAC 2 cut(s) 150, 1247
Mox20I TGGCCA 2 cut(s) 500, 1863
Mph1103I ATGCAT 1 cut(s) 1012
MroI TCCGGA 1 cut(s) 1336
MroXI GAANNNNTTC 1 cut(s) 1308
MscI TGGCCA 2 cut(s) 500, 1863
MseI TTAA 9 cut(s) 135, 284, 527, 1046, 1182, 1251, 1814, 1881, 1912
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 500, 1863
MspA1I CMGCKG 3 cut(s) 350, 1075, 1604
MspI CCGG 5 cut(s) 381, 405, 771, 1211, 1337
MspR9I CCNGG 5 cut(s) 222, 822, 939, 1212, 1645
MunI CAATTG 2 cut(s) 1343, 1734
Mva1269I GAATGC 1 cut(s) 1388
MvaI CCWGG 4 cut(s) 222, 822, 939, 1645
MvnI CGCG 1 cut(s) 1494
MwoI GCNNNNNNNGC 2 cut(s) 1016, 1901
NciI CCSGG 1 cut(s) 1212
NcoI CCATGG 1 cut(s) 1547
NdeI CATATG 2 cut(s) 80, 1012
NdeII GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
NheI GCTAGC 1 cut(s) 806
NlaIII CATG 4 cut(s) 27, 229, 583, 1551
NlaIV GGNNCC 3 cut(s) 535, 769, 1545
NmuCI GTSAC 1 cut(s) 26
NsbI TGCGCA 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 1 cut(s) 229
NspV TTCGAA 1 cut(s) 1295
PaeR7I CTCGAG 1 cut(s) 260
PctI GAATGC 1 cut(s) 1388
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 8 cut(s) 256, 371, 686, 783, 924, 970, 1058, 1856
PfoI TCCNGGA 1 cut(s) 820
PkrI GCNGC 8 cut(s) 349, 619, 639, 679, 946, 1205, 1741, 1744
PleI GAGTC 2 cut(s) 1252, 1839
PpsI GAGTC 2 cut(s) 1252, 1839
PsiI TTATAA 1 cut(s) 299
Psp6I CCWGG 4 cut(s) 220, 820, 937, 1643
PspGI CCWGG 4 cut(s) 220, 820, 937, 1643
PspN4I GGNNCC 3 cut(s) 535, 769, 1545
PspPI GGNCC 2 cut(s) 905, 1339
PstNI CAGNNNCTG 3 cut(s) 350, 485, 1458
PsuI RGATCY 3 cut(s) 533, 625, 1068
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 5 cut(s) 467, 901, 934, 1584, 1617
RsaNI GTAC 5 cut(s) 466, 900, 933, 1583, 1616
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 9 cut(s) 135, 284, 527, 1046, 1182, 1251, 1814, 1881, 1912
SatI GCNGC 8 cut(s) 348, 618, 638, 678, 945, 1204, 1740, 1743
Sau3AI GATC 9 cut(s) 19, 533, 564, 625, 844, 881, 1068, 1486, 1516
Sau96I GGNCC 2 cut(s) 905, 1339
ScaI AGTACT 1 cut(s) 1584
SchI GAGTC 2 cut(s) 1252, 1839
ScrFI CCNGG 5 cut(s) 222, 822, 939, 1212, 1645
SfaNI GCATC 1 cut(s) 140
Sfr274I CTCGAG 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 1295
SinI GGWCC 2 cut(s) 905, 1339
SlaI CTCGAG 1 cut(s) 260
SmiI ATTTAAAT 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 2 cut(s) 154, 260
SmoI CTYRAG 2 cut(s) 154, 260
SsiI CCGC 6 cut(s) 678, 962, 1075, 1126, 1604, 1743
SspMI CTAG 3 cut(s) 807, 1371, 1775
StyD4I CCNGG 5 cut(s) 220, 820, 937, 1210, 1643
StyI CCWWGG 5 cut(s) 107, 393, 908, 1547, 1864
SwaI ATTTAAAT 1 cut(s) 528
TaaI ACNGT 9 cut(s) 32, 44, 168, 450, 487, 917, 1155, 1694, 1719
TaqI TCGA 7 cut(s) 261, 308, 548, 714, 884, 1295, 1320
TatI WGTACW 1 cut(s) 1582
TauI GCSGC 2 cut(s) 680, 1745
TfiI GAWTC 8 cut(s) 256, 371, 686, 783, 924, 970, 1058, 1856
Tru1I TTAA 9 cut(s) 135, 284, 527, 1046, 1182, 1251, 1814, 1881, 1912
Tru9I TTAA 9 cut(s) 135, 284, 527, 1046, 1182, 1251, 1814, 1881, 1912
TscAI CASTG 5 cut(s) 151, 1105, 1160, 1290, 1722
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 6 cut(s) 347, 617, 637, 944, 1203, 1739
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 6 cut(s) 227, 269, 596, 761, 1461, 1819
TspGWI ACGGA 2 cut(s) 570, 740
TspRI CASTG 5 cut(s) 151, 1105, 1160, 1290, 1722
VpaK11BI GGWCC 2 cut(s) 905, 1339
XapI RAATTY 2 cut(s) 233, 1826
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 1308
XspI CTAG 3 cut(s) 807, 1371, 1775
ZrmI AGTACT 1 cut(s) 1584
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.