Rw5G036580

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001151
Physical Location & Seq
Reverse (-)
64154392 .. 64158093
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036580.1

Sequence Viewer

Length: 2418 bp
ATGAAGGGCCTCATCTTCATCTTCATCTCTCTATTCCTATTTTTAGCCTTGAATTTTTCCACTGCAGCTGATACCATATCACCATCACAATCCATCACCGGCTCGAATACCCTGGTTTCTTCCAACCAGAGATTTGTGCTAGGACTCTTCTCCCCACCCAATTCTAGAGCATGGTATTTAGGACTATGGTACAAGAAATTTCCAGATGTTGTTGTATGGATTGCAAACAGAGAAAACCCACTTCCAGGTTCGAATGGAGCCTTGACATTATCAGAAAAAGGCACCCTCATCCTTTCAGACCAAATGAACAAGACAATTTGGTCCTCTAATTCTTCCAGAGTAGCCAAAAACCCAGTTGCGCAGCTTTTGGTGACTGGAAATCTTGTTGTCAGAGACCAGGCGTCTCCAACAGCAACAGCAGAGTCCGAAAGCTATGTTTTTCAAAGCTTTGATTTCCCATCAAACACGCTTTTACCAGATATGAAGGTGGGGTGGGACTTCAGAACTGGCCAAGACCATTTCTTGACTAGTTGGAAAAATGCTAGCGATCCCTCCCCTGGAGAATATACCTATAGGATTGAAAACCTTGAGTTGCCTCAGCTTGTTGTCGCTCAAGGATCAAAGAAAGTGTTCAGGACAGGGCCTTGGAACGGTCTTCGATTTACAGGTACTCCTGTTTCGGTTGTTACAGCAACATCATTTTTGGTACCAATCTTTGTCTACAATACCAAGGAGCTGTACTACTCATATGAGGTTACTGATGATTCTATTCTGACAAGAGTGACGCTGACTGAGTCGGGTTTGGCTCAGCGGCTAGTGATGAATGAAGGGAGCAGTGAATGGGCTGTTATGTACACTCTGCAGAACGATCAGTGTGATGATTATGGTCAGTGTGGGCCAAATGGCATTTGCAAGATTTACAAATCACCCATTTGTGAATGCTTGCAGGGGTTTGTTCCCAAATCTCCAAAGGAATGGGAGTTTCTTAACTGGACAACCGGGTGCACGAGGAGGACAGCACTGCATTGTGAGAAAGGGGAAGGATTTGTGAAAGTTAATAATGTGAAACTGCCGGACTTGTTGGATTTTCGGGTGAACAAAACCATGCTCCGCGGAGAGGAATGTGAGGCAGAGTGTTTGAGGAGCTGCTCCTGTATGGCTTATGCCAATTCGAACATAACCAATGGAGGCAAAGGATGTCTTATGTGGTTTGGCAAGCTGATTGATATGCGGGAGATCGCTGAAGATGGTGACCAGATATATATTAGGATGCCAGCTTCCGAACTAGGTGATACCAGCCAGAAACATAAAAGAGTATTGCTCGTCTCGGTCATATCATCAGCAGGTTTTGTGCTTCTTTTTCTGGGTTTCTCATGCTGCTATATTTATGTGAAGAAGAGAGAAAAGAAAATGGGCTCAAGAACTAGTGAGGAGGGCTTGGAGTTGCCACTGTTTGATTATGGTAGAATTGCAACCTCCACCAATGACTTCTCCGGGACAAACAAACTGGGAGAGGGAGGTTTTGGTCCAGTTTACAAGGCCATGTTATCACAAGAAGAGTTTGTAGCAGTAAAGAGACTCTCGAAAGATTCTGGGCAAGGTGTTGAAGAGTTTAAGAACGAAGTTACAATGATGGCTAATCTTCAACACTGGAATCTTGTTAAACTTCTGGGCTGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCCAACAAAAGCTTGGACTGCTTTATTTTCGGTCTAAGATTCTACCCTCTAATCTTTATGCATTTAAGAAACGATTACCTACACCAAGACTCCAGGCTAAGAATCATTCATAGGGACCTTAAGAGTAGCAACATCTTACTCGACGATGAGCTGAACCCCAAAATTTCTGACTTTGGTATAGCAAGAATTTTCGGACGCAATCAGAATGAAGCTAAAACAAAACGAGTAATTGGCACATACGGATATATGTCCCCGGAGTATGCAATTGATGGAAAATTTTCAGTGAAATCTGATGTCTTCAGCTTTGGAGTGCTTTTGTTAGAGATAGTAAGCGGCAGAAAGAACAGAGGGTTTCATCATCCTGATCACCATCACAATCTTTTGGGACATGCATGGTTATTGTGGAAACAAAACAAGGGTTTGGAGCTAATGGATATATGCCTGGAGGACTCATATGTTGAGTTTGAATTGCTAAGGTGCATTCAAGTGGGTCTGTTGTGTGTTCAAAAGCTGCCTGAAGACAGACCAGTAATGTCATCGGTGGTTCTTATGTTAAGCAATGAGGGAGCAACTTTGCCTCAGCCAAAGAAGCCTGGATTCTTTATAGAAAGAGGTTCCTTGGACTTTGATAGCTTAAGAGACCCAGGAAAAAGCAACACAGGGAACACAATTACCATTACCACAGTGGAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

805

Amino Acids

90.62

Weight (kDa)

6.54

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 179 1.3e-32 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 4.6e-28 S-locus glycoprotein domain
PAN_2 PF08276 343 - 410 2e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 500 - 688 8.8e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 500 - 687 3.3e-38 Protein kinase domain
DUF3403 PF11883 760 - 805 4.9e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 1334
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 281, 706
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 1113
AciI CCGC 5 cut(s) 811, 1111, 1113, 1231, 2052
AclWI GGATC 2 cut(s) 542, 625
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 5 cut(s) 52, 197, 1881, 1905, 1994
AcuI CTGAAG 4 cut(s) 484, 1263, 2002, 2256
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 6 cut(s) 191, 670, 708, 740, 854, 1715
AfiI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
AflII CTTAAG 2 cut(s) 1838, 2353
AgsI TTSAA 9 cut(s) 52, 443, 581, 1607, 1646, 1685, 2186, 2204, 2225
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 2 cut(s) 527, 1424
AjnI CCWGG 8 cut(s) 111, 244, 396, 556, 1811, 2160, 2311, 2362
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 5 cut(s) 387, 408, 1330, 1570, 2352
Alw44I GTGCAC 1 cut(s) 1003
AlwI GGATC 2 cut(s) 542, 625
AoxI GGCC 5 cut(s) 7, 508, 641, 896, 1539
ApaLI GTGCAC 1 cut(s) 1003
ApeKI GCWGC 7 cut(s) 65, 361, 1146, 1377, 1674, 1677, 2230
ApoI RAATTY 5 cut(s) 52, 197, 1881, 1905, 1994
Asp700I GAANNNNTTC 2 cut(s) 629, 1996
Asp718I GGTACC 1 cut(s) 706
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 6 cut(s) 7, 321, 641, 896, 1526, 1834
AsuC2I CCSGG 3 cut(s) 1000, 1495, 1973
AsuHPI GGTGA 8 cut(s) 72, 88, 382, 918, 1105, 1262, 1301, 2078
AsuII TTCGAA 2 cut(s) 251, 1172
AsuNHI GCTAGC 1 cut(s) 542
AvaII GGWCC 3 cut(s) 321, 1526, 1834
BaeGI GKGCMC 1 cut(s) 1007
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 2 cut(s) 281, 706
BanII GRGCYC 1 cut(s) 1418
BauI CACGAG 1 cut(s) 1006
BbsI GAAGAC 3 cut(s) 647, 2008, 2244
Bbv12I GWGCWC 1 cut(s) 1007
BbvCI CCTCAGC 2 cut(s) 597, 2298
BbvI GCAGC 7 cut(s) 77, 373, 1133, 1364, 1661, 1664, 2217
BccI CCATC 7 cut(s) 91, 101, 466, 1241, 1627, 1982, 2097
BciT130I CCWGG 8 cut(s) 113, 246, 398, 558, 1813, 2162, 2313, 2364
BclI TGATCA 1 cut(s) 2083
BcnI CCSGG 3 cut(s) 1000, 1495, 1973
BcoDI GTCTC 5 cut(s) 387, 408, 1330, 1570, 2352
BcuI ACTAGT 2 cut(s) 527, 1424
BfaI CTAG 8 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 2412
BfmI CTRYAG 3 cut(s) 63, 571, 860
BfrI CTTAAG 2 cut(s) 1838, 2353
BfuAI ACCTGC 1 cut(s) 1334
BisI GCNGC 9 cut(s) 66, 362, 812, 1147, 1378, 1675, 1678, 2053, 2231
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 9 cut(s) 67, 363, 813, 1148, 1379, 1676, 1679, 2054, 2232
Bme18I GGWCC 3 cut(s) 321, 1526, 1834
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 6 cut(s) 7, 321, 641, 896, 1526, 1834
BmiI GGNNCC 5 cut(s) 259, 283, 708, 1835, 2335
BmrI ACTGGG 2 cut(s) 347, 1517
BmsI GCATC 2 cut(s) 1260, 1689
BmtI GCTAGC 1 cut(s) 546
BmuI ACTGGG 2 cut(s) 347, 1517
BpiI GAAGAC 3 cut(s) 647, 2008, 2244
BplI GAGNNNNNCTC 2 cut(s) 1305, 1337
BpmI CTGGAG 3 cut(s) 579, 1795, 2183
Bpu10I CCTNAGC 3 cut(s) 597, 2192, 2298
Bpu1102I GCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 2 cut(s) 251, 1172
BpuEI CTTGAG 3 cut(s) 597, 608, 1402
BpuMI CCSGG 3 cut(s) 1000, 1495, 1973
BsaBI GATNNNNATC 2 cut(s) 1703, 2088
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 2 cut(s) 387, 2352
BsaJI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1971, 2337, 2362
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 1793, 1823
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 8 cut(s) 353, 379, 511, 995, 1512, 1529, 1655, 2246
Bse3DI GCAATG 1 cut(s) 2284
Bse8I GATNNNNATC 2 cut(s) 1703, 2088
BseBI CCWGG 8 cut(s) 113, 246, 398, 558, 1813, 2162, 2313, 2364
BseDI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1971, 2337, 2362
BseGI GGATG 5 cut(s) 288, 1202, 1275, 1704, 2077
BseJI GATNNNNATC 2 cut(s) 1703, 2088
BseLI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
BseMI GCAATG 1 cut(s) 2284
BseMII CTCAG 4 cut(s) 611, 783, 821, 2312
BseNI ACTGG 8 cut(s) 353, 379, 511, 995, 1512, 1529, 1655, 2246
BseRI GAGGAG 3 cut(s) 1024, 1156, 1445
BseSI GKGCMC 1 cut(s) 1007
BseXI GCAGC 7 cut(s) 77, 373, 1133, 1364, 1661, 1664, 2217
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 5 cut(s) 9, 510, 643, 898, 1541
BshNI GGYRCC 2 cut(s) 281, 706
BsiHKAI GWGCWC 1 cut(s) 1007
BsiSI CCGG 5 cut(s) 99, 999, 1073, 1494, 1973
BslFI GGGAC 5 cut(s) 509, 1510, 1847, 1954, 2118
BslI CCNNNNNNNGG 4 cut(s) 245, 557, 650, 1117
BsmAI GTCTC 5 cut(s) 387, 408, 1330, 1570, 2352
BsmBI CGTCTC 2 cut(s) 408, 1330
BsmFI GGGAC 5 cut(s) 509, 1510, 1847, 1954, 2118
BsmI GAATGC 2 cut(s) 944, 2199
BsnI GGCC 5 cut(s) 9, 510, 643, 898, 1541
Bso31I GGTCTC 2 cut(s) 387, 2352
Bsp119I TTCGAA 2 cut(s) 251, 1172
Bsp1286I GDGCHC 2 cut(s) 1007, 1418
Bsp1407I TGTACA 1 cut(s) 852
Bsp143I GATC 5 cut(s) 547, 617, 868, 1236, 2083
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 5 cut(s) 811, 1111, 1113, 1231, 2052
BspANI GGCC 5 cut(s) 9, 510, 643, 898, 1541
BspCNI CTCAG 4 cut(s) 610, 784, 820, 2311
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 5 cut(s) 259, 283, 708, 1835, 2335
BspMAI CTGCAG 2 cut(s) 67, 864
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 542, 625
BspT104I TTCGAA 2 cut(s) 251, 1172
BspT107I GGYRCC 2 cut(s) 281, 706
BspTI CTTAAG 2 cut(s) 1838, 2353
BspTNI GGTCTC 2 cut(s) 387, 2352
BsrDI GCAATG 1 cut(s) 2284
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 852
BsrI ACTGG 8 cut(s) 353, 379, 511, 995, 1512, 1529, 1655, 2246
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 8 cut(s) 111, 556, 644, 729, 1111, 1971, 2337, 2362
BssMI GATC 5 cut(s) 547, 617, 868, 1236, 2083
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 1006
BssT1I CCWWGG 3 cut(s) 644, 729, 2337
Bst2BI CACGAG 1 cut(s) 1006
Bst2UI CCWGG 8 cut(s) 113, 246, 398, 558, 1813, 2162, 2313, 2364
Bst4CI ACNGT 3 cut(s) 653, 1452, 2404
Bst6I CTCTTC 4 cut(s) 152, 1391, 1551, 1602
BstACI GRCGYC 1 cut(s) 401
BstAFI CTTAAG 2 cut(s) 1838, 2353
BstAUI TGTACA 1 cut(s) 852
BstBI TTCGAA 2 cut(s) 251, 1172
BstC8I GCNNGC 4 cut(s) 544, 944, 1217, 1275
BstDEI CTNAG 7 cut(s) 597, 792, 807, 1754, 1817, 2192, 2298
BstDSI CCRYGG 1 cut(s) 1111
BstEII GGTNACC 1 cut(s) 1250
BstF5I GGATG 5 cut(s) 288, 1202, 1275, 1704, 2077
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 5 cut(s) 550, 620, 871, 1239, 2086
BstMAI GTCTC 5 cut(s) 387, 408, 1330, 1570, 2352
BstMBI GATC 5 cut(s) 547, 617, 868, 1236, 2083
BstMWI GCNNNNNNNGC 2 cut(s) 1737, 2308
BstNI CCWGG 8 cut(s) 113, 246, 398, 558, 1813, 2162, 2313, 2364
BstNSI RCATGY 2 cut(s) 1720, 2111
BstPI GGTNACC 1 cut(s) 1250
BstSFI CTRYAG 3 cut(s) 63, 571, 860
BstSLI GKGCMC 1 cut(s) 1007
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 7 cut(s) 77, 373, 1133, 1364, 1661, 1664, 2217
BstV2I GAAGAC 3 cut(s) 647, 2008, 2244
BstXI CCANNNNNNTGG 1 cut(s) 975
BsuRI GGCC 5 cut(s) 9, 510, 643, 898, 1541
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 5 cut(s) 288, 1202, 1275, 1704, 2077
BtsI GCAGTG 3 cut(s) 60, 841, 1019
BtsIMutI CAGTG 9 cut(s) 60, 841, 878, 896, 1019, 1448, 1648, 2007, 2409
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 4 cut(s) 544, 944, 1217, 1275
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 6 cut(s) 7, 321, 641, 896, 1526, 1834
Cfr42I CCGCGG 1 cut(s) 1114
CseI GACGC 3 cut(s) 390, 793, 1923
Csp6I GTAC 6 cut(s) 190, 669, 707, 739, 853, 1714
CviAII CATG 7 cut(s) 171, 1105, 1374, 1543, 1717, 2108, 2112
CviQI GTAC 6 cut(s) 190, 669, 707, 739, 853, 1714
DdeI CTNAG 7 cut(s) 597, 792, 807, 1754, 1817, 2192, 2298
DpnI GATC 5 cut(s) 549, 619, 870, 1238, 2085
DpnII GATC 5 cut(s) 547, 617, 868, 1236, 2083
DrdI GACNNNNNNGTC 1 cut(s) 319
DriI GACNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 4 cut(s) 152, 1391, 1551, 1602
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 4 cut(s) 152, 1391, 1551, 1602
Eco130I CCWWGG 3 cut(s) 644, 729, 2337
Eco24I GRGCYC 1 cut(s) 1418
Eco31I GGTCTC 2 cut(s) 387, 2352
Eco47I GGWCC 3 cut(s) 321, 1526, 1834
Eco57I CTGAAG 4 cut(s) 484, 1263, 2002, 2256
Eco91I GGTNACC 1 cut(s) 1250
EcoO109I RGGNCCY 3 cut(s) 7, 641, 1834
EcoO65I GGTNACC 1 cut(s) 1250
EcoRII CCWGG 8 cut(s) 111, 244, 396, 556, 1811, 2160, 2311, 2362
EcoT14I CCWWGG 3 cut(s) 644, 729, 2337
EcoT22I ATGCAT 2 cut(s) 1782, 2113
EcoT38I GRGCYC 1 cut(s) 1418
ErhI CCWWGG 3 cut(s) 644, 729, 2337
Esp3I CGTCTC 2 cut(s) 408, 1330
FaeI CATG 7 cut(s) 174, 1108, 1377, 1546, 1720, 2111, 2115
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 5 cut(s) 509, 1510, 1847, 1954, 2118
FatI CATG 7 cut(s) 170, 1104, 1373, 1542, 1716, 2107, 2111
FauI CCCGC 1 cut(s) 1224
FauNDI CATATG 2 cut(s) 748, 2173
FbaI TGATCA 1 cut(s) 2083
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 9 cut(s) 66, 362, 812, 1147, 1378, 1675, 1678, 2053, 2231
FokI GGATG 5 cut(s) 275, 1209, 1282, 1711, 2064
FriOI GRGCYC 1 cut(s) 1418
Fsp4HI GCNGC 9 cut(s) 66, 362, 812, 1147, 1378, 1675, 1678, 2053, 2231
FspBI CTAG 8 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 2412
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GluI GCNGC 9 cut(s) 66, 362, 812, 1147, 1378, 1675, 1678, 2053, 2231
GsuI CTGGAG 3 cut(s) 579, 1795, 2183
HaeIII GGCC 5 cut(s) 9, 510, 643, 898, 1541
HapII CCGG 5 cut(s) 99, 999, 1073, 1494, 1973
HgaI GACGC 3 cut(s) 390, 793, 1923
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 7 cut(s) 174, 1108, 1377, 1546, 1720, 2111, 2115
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HindIII AAGCTT 2 cut(s) 445, 1729
HpaII CCGG 5 cut(s) 99, 999, 1073, 1494, 1973
HphI GGTGA 8 cut(s) 72, 88, 382, 918, 1105, 1262, 1301, 2078
Hpy166II GTNNAC 5 cut(s) 721, 855, 1005, 1096, 1534
Hpy188III TCNNGA 8 cut(s) 165, 203, 336, 523, 634, 1419, 1582, 2081
Hpy8I GTNNAC 5 cut(s) 721, 855, 1005, 1096, 1534
Hpy99I CGWCG 1 cut(s) 1865
HpyAV CCTTC 4 cut(s) 478, 821, 1034, 1679
HpyCH4III ACNGT 3 cut(s) 653, 1452, 2404
HpyF10VI GCNNNNNNNGC 2 cut(s) 1737, 2308
HpyF3I CTNAG 7 cut(s) 597, 792, 807, 1754, 1817, 2192, 2298
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 7 cut(s) 174, 1108, 1377, 1546, 1720, 2111, 2115
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 710
Ksp22I TGATCA 1 cut(s) 2083
KspI CCGCGG 1 cut(s) 1114
Kzo9I GATC 5 cut(s) 547, 617, 868, 1236, 2083
LmnI GCTCC 8 cut(s) 257, 733, 831, 1113, 1143, 1154, 2143, 2285
Lsp1109I GCAGC 7 cut(s) 77, 373, 1133, 1364, 1661, 1664, 2217
LweI GCATC 2 cut(s) 1260, 1689
MaeI CTAG 8 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 2412
MaeIII GTNAC 6 cut(s) 370, 685, 754, 781, 1250, 1624
MalI GATC 5 cut(s) 549, 619, 870, 1238, 2085
MboI GATC 5 cut(s) 547, 617, 868, 1236, 2083
MfeI CAATTG 1 cut(s) 1983
MhlI GDGCHC 2 cut(s) 1007, 1418
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 6 cut(s) 138, 431, 803, 1572, 1802, 2162
MmeI TCCRAC 4 cut(s) 147, 431, 512, 1062
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 2 cut(s) 1782, 2113
MroXI GAANNNNTTC 2 cut(s) 629, 1996
MscI TGGCCA 1 cut(s) 510
MseI TTAA 8 cut(s) 987, 1056, 1614, 1662, 1784, 1839, 2273, 2354
MslI CAYNNNNRTG 1 cut(s) 2204
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 68, 811, 1113
MspCI CTTAAG 2 cut(s) 1838, 2353
MspI CCGG 5 cut(s) 99, 999, 1073, 1494, 1973
MunI CAATTG 1 cut(s) 1983
Mva1269I GAATGC 2 cut(s) 944, 2199
MvaI CCWGG 8 cut(s) 113, 246, 398, 558, 1813, 2162, 2313, 2364
MvnI CGCG 1 cut(s) 1113
MwoI GCNNNNNNNGC 2 cut(s) 1737, 2308
NciI CCSGG 3 cut(s) 1000, 1495, 1973
NdeI CATATG 2 cut(s) 748, 2173
NdeII GATC 5 cut(s) 547, 617, 868, 1236, 2083
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 7 cut(s) 174, 1108, 1377, 1546, 1720, 2111, 2115
NlaIV GGNNCC 5 cut(s) 259, 283, 708, 1835, 2335
NmuCI GTSAC 3 cut(s) 370, 781, 1250
NsbI TGCGCA 1 cut(s) 360
NsiI ATGCAT 2 cut(s) 1782, 2113
NspI RCATGY 2 cut(s) 1720, 2111
NspV TTCGAA 2 cut(s) 251, 1172
PctI GAATGC 2 cut(s) 944, 2199
PdmI GAANNNNTTC 2 cut(s) 629, 1996
PfeI GAWTC 6 cut(s) 764, 1589, 1654, 1758, 1821, 2316
PflFI GACNNNGTC 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 1493
PkrI GCNGC 9 cut(s) 67, 363, 813, 1148, 1379, 1676, 1679, 2054, 2232
PleI GAGTC 6 cut(s) 138, 430, 802, 1572, 1802, 2162
PpsI GAGTC 6 cut(s) 138, 430, 802, 1572, 1802, 2162
PpuMI RGGWCCY 1 cut(s) 1834
Psp5II RGGWCCY 1 cut(s) 1834
Psp6I CCWGG 8 cut(s) 111, 244, 396, 556, 1811, 2160, 2311, 2362
PspEI GGTNACC 1 cut(s) 1250
PspGI CCWGG 8 cut(s) 111, 244, 396, 556, 1811, 2160, 2311, 2362
PspN4I GGNNCC 5 cut(s) 259, 283, 708, 1835, 2335
PspPI GGNCC 6 cut(s) 7, 321, 641, 896, 1526, 1834
PspPPI RGGWCCY 1 cut(s) 1834
PstI CTGCAG 2 cut(s) 67, 864
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 6 cut(s) 191, 670, 708, 740, 854, 1715
RsaNI GTAC 6 cut(s) 190, 669, 707, 739, 853, 1714
RseI CAYNNNNRTG 1 cut(s) 2204
SacII CCGCGG 1 cut(s) 1114
SaqAI TTAA 8 cut(s) 987, 1056, 1614, 1662, 1784, 1839, 2273, 2354
SatI GCNGC 9 cut(s) 66, 362, 812, 1147, 1378, 1675, 1678, 2053, 2231
Sau3AI GATC 5 cut(s) 547, 617, 868, 1236, 2083
Sau96I GGNCC 6 cut(s) 7, 321, 641, 896, 1526, 1834
SchI GAGTC 6 cut(s) 138, 431, 803, 1572, 1802, 2162
SduI GDGCHC 2 cut(s) 1007, 1418
SfaNI GCATC 2 cut(s) 1260, 1689
SfcI CTRYAG 3 cut(s) 63, 571, 860
Sfr303I CCGCGG 1 cut(s) 1114
SfuI TTCGAA 2 cut(s) 251, 1172
SgrBI CCGCGG 1 cut(s) 1114
SinI GGWCC 3 cut(s) 321, 1526, 1834
SmiMI CAYNNNNRTG 1 cut(s) 2204
SmlI CTYRAG 5 cut(s) 587, 612, 1417, 1838, 2353
SmoI CTYRAG 5 cut(s) 587, 612, 1417, 1838, 2353
SpeI ACTAGT 2 cut(s) 527, 1424
SsiI CCGC 5 cut(s) 811, 1111, 1113, 1231, 2052
SspMI CTAG 8 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 2412
StyI CCWWGG 3 cut(s) 644, 729, 2337
TaaI ACNGT 3 cut(s) 653, 1452, 2404
TaqI TCGA 6 cut(s) 104, 251, 658, 1172, 1583, 1860
TaqII GACCGA 2 cut(s) 1318, 1739
TatI WGTACW 3 cut(s) 738, 852, 1713
TauI GCSGC 2 cut(s) 814, 2055
TfiI GAWTC 6 cut(s) 764, 1589, 1654, 1758, 1821, 2316
Tru1I TTAA 8 cut(s) 987, 1056, 1614, 1662, 1784, 1839, 2273, 2354
Tru9I TTAA 8 cut(s) 987, 1056, 1614, 1662, 1784, 1839, 2273, 2354
TscAI CASTG 9 cut(s) 67, 841, 878, 896, 1026, 1455, 1655, 2007, 2409
TseFI GTSAC 3 cut(s) 370, 781, 1250
TseI GCWGC 7 cut(s) 65, 361, 1146, 1377, 1674, 1677, 2230
Tsp45I GTSAC 3 cut(s) 370, 781, 1250
TspGWI ACGGA 1 cut(s) 1974
TspRI CASTG 9 cut(s) 67, 841, 878, 896, 1026, 1455, 1655, 2007, 2409
Tth111I GACNNNGTC 1 cut(s) 793
Vha464I CTTAAG 2 cut(s) 1838, 2353
VneI GTGCAC 1 cut(s) 1003
VpaK11BI GGWCC 3 cut(s) 321, 1526, 1834
XapI RAATTY 5 cut(s) 52, 197, 1881, 1905, 1994
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 2 cut(s) 1720, 2111
XcmI CCANNNNNNNNNTGG 2 cut(s) 1729, 2401
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 629, 1996
XspI CTAG 8 cut(s) 140, 165, 528, 543, 815, 1286, 1425, 2412
Zsp2I ATGCAT 2 cut(s) 1782, 2113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.