RchiOBHm_Chr5g0036341

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001151
Physical Location & Seq
Forward (+)
30531366 .. 30534768
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31516

Sequence Viewer

Length: 2451 bp
ATGAAGGGCCTTCCTTTCATCTTATTTTTCTCTCTTTTCCATGTCTTAGTGTTCAGTTTATGCAGTGCAGTTGATACTATATCTCCAACACAATCCATCACCCGCTCCAATACCTTGGTTTCTTCCGGTGGAAGATTTGAATTAGGACTCTTCCCCTCCGGAAACTCTAAAGCATGGTATTTTGGAATTCGGTACACCAAGTTTTCGAATATAACCGTATGGGTCGCCAACAGAGAAAACCCAATTCCAGGTTCAGATGGAGCCCTGACATTAACCGAAAATGGTACTTTGGTCCTTTTAGACCAGATGAACAACACTGTATGGTATTCTAATTTTTCCACAGTAGTTGAAGATCCTGTTGCACAGCTTTTGGAGACTGGAAATTTTGTTGTCAGAGACAAGGCTACAGCGGGTTCGGAAAGTTATGTATGGCAGAGTTTTGATTTCCCATCGGATACATTGTTAGCGGGGATGAAGCTTGGATGGGATTTCAGAACCGGGCTCAACAAGTTCTTGACTTCTTGGAAAAATGCTAGTGATCCATCTCTTGGTGAGTATACCTATAGGATTGTTAATCTTGCTTTGCCTCAGCTTGTTCTTGCTAAGGGATCGGAGAAAGAGTTTCGAAGTGGGCCCTGGAATGGTCTTCGGTTTACTGGTGTTCCTGTTTCGAACAATGAAATTATAACACCAAGTTATGTGTATGATACAAATGAGTTGTATTACACGTATGAGACTAATGACAATTCTCATATTACAAGATTGCAGCTGACTGAAGGGGGCGACATGAAGCGGTTAGTGCTGAATGAAGGAAGCACTGAATGGAACGATATGTATAATTTGCTGAATGATCGATGTGATAGTTACGGGGAGTGTGGCGCAAATGGCATTTGCAGAATTAACAACTCACCCATTTGTGACTGCTTGGAGGGGTTTATTCCGAAATCCCAGCAAGAGTGGGAGGTGCTTAATACGACAAGTGGGTGCACGAGGAAGATACAACTGGATTGCCAGAAGGGAGAAGGGTTTCGGAAAATTCAAAATGTAAAACTGCCAGACCTGTTGGATTTTTGGGTCAACAAGAACCTGAACAAGAAGGAATGCAGGGCAGAGTGCTTGAAAAATTGTTCTTGTATTGCTTATGCTTATTCAGACTACAGTCTTGGAGGCACCGGCTGTCTGATGTGGTTTAAAGACCTCATTGATATCCGAGAGTTTGTTGATGAAAGTGAGCAACATATTTATATTAGGATGCCAGCATCAGAACTAGGTCATGCCGGTCAGAAGGAGAAAAGAATAGTGCTCATAGCGGTCATCTCAGCAGCTTGTGTTCTAATTTTGCTGGGAGTGTCATGTTGGCTTATAATTTGGAAGAAGAAAGGAAAGAAGAGGGGTTCAAGATCGAATAAGGAAGACATGGAGTTGCCACTGTTTGATTTTCATACAATTGCAGCAGGCACCAATAACTTCTCAAGGACAAACATACTTGGGGAGGGAGGTTTTGGTCCAGTTTACAAGGCCAACCTAGCACAAGAACAACTAGTAGCAGTAAAAAGACTCTCAAAAGATTCCGGGCAAGGTATTGATGAGTTTAAGAATGAAGTTACGATGATAGCCCACCTTCAACACAGGAATCTTGTTAAACTTTTGGGCTGCTGCATTGAAGGACAAGAGAGGATGCTAATCTATGAGTATATGCCTAATAAAAGCTTGGACTGCTTTATTTTCGATGAAAATAGAAAGAAATCGCTTGATTGGCAAAAACGAGTCAACATTATAATTGGAACTGCACGAGGACTTCTCTACCTCCACCAAGACTCCAGATTGAGAATCATTCATAGGGACCTCAAGAGTAGCAACATTTTACTCGACAATGAGCTGAACCCCAAAATATCTGACTTCGGCATAGCAAGAATTTTTGGACTTGATCAGACTGAAGCAAAGACAAAACGAGTGATTGGCACGTATGGGTATATGTCTCCCGAGTACGCAATTGATGGGCACTTTTCAGTGAAATCCGATGTCTTTAGTTTTGGAGTGCTTTTGCTAGAGATAGTAAGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGATCACCATCACAGTCTTCTGGGACATGAATGTGATTTGCAAATGTCCCAGGCCTGGCTACTATGGAACAAAGAAAAGGTTTTGGAACTATTAGATCCATGCTTGGAGGACTCATATGTTGAGTTTGAGGTACTAAGATGTATACAAGTTGGTCTATTATGCGTGCAAAAGCGGCCCATCGACAGACCAGTAATGTCATCAGTGGTGTTCATGTTAAGCAACGAGGGAGCAATGTTGCCTGAACCGAAGGAGCCTGGTTTCTTCACAGAACGAAGTTCCATGGATGGTGATACCTTCATTAGTGAAGGAAGAAGCAAAACAGGAAACATGATTACCATTACCACGATGGAAGCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

816

Amino Acids

92.26

Weight (kDa)

6.12

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 176 1.6e-34 D-mannose binding lectin
S_locus_glycop PF00954 208 - 316 6.1e-28 S-locus glycoprotein domain
PAN_2 PF08276 337 - 403 9.2e-22 PAN-like domain
Pkinase PF00069 491 - 699 2.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 692 1.7e-46 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 772 - 816 8.4e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 686, 1364, 1778
AccB1I GGYRCC 2 cut(s) 1169, 1457
AccB7I CCANNNNNTGG 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 2 cut(s) 557, 2233
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 6 cut(s) 103, 410, 467, 793, 1310, 2263
AclWI GGATC 4 cut(s) 347, 533, 616, 2180
AcsI RAATTY 4 cut(s) 186, 382, 1035, 1914
AcuI CTGAAG 2 cut(s) 795, 1956
AfaI GTAC 4 cut(s) 194, 286, 1988, 2223
AfiI CCNNNNNNNGG 4 cut(s) 248, 548, 641, 2145
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 7 cut(s) 140, 350, 1040, 1120, 1398, 1625, 1664
AhlI ACTAGT 1 cut(s) 1540
AjnI CCWGG 5 cut(s) 247, 635, 2139, 2144, 2344
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AluBI AGCT 8 cut(s) 367, 478, 592, 769, 1325, 1710, 1879, 2444
AluI AGCT 8 cut(s) 367, 478, 592, 769, 1325, 1710, 1879, 2444
Alw21I GWGCWC 2 cut(s) 989, 1305
Alw26I GTCTC 4 cut(s) 368, 390, 728, 1983
Alw44I GTGCAC 1 cut(s) 985
AlwI GGATC 4 cut(s) 347, 533, 616, 2180
Ama87I CYCGRG 1 cut(s) 1982
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 5 cut(s) 7, 632, 1518, 2142, 2264
ApaI GGGCCC 1 cut(s) 636
ApaLI GTGCAC 1 cut(s) 985
ApeKI GCWGC 5 cut(s) 766, 1322, 1451, 1653, 1656
ApoI RAATTY 4 cut(s) 186, 382, 1035, 1914
Asp700I GAANNNNTTC 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 7 cut(s) 7, 292, 632, 633, 1505, 1843, 2265
AsuC2I CCSGG 2 cut(s) 499, 1573
AsuHPI GGTGA 5 cut(s) 91, 563, 900, 2087, 2390
AsuII TTCGAA 3 cut(s) 206, 625, 671
AvaI CYCGRG 1 cut(s) 1982
AvaII GGWCC 3 cut(s) 292, 1505, 1843
BaeGI GKGCMC 3 cut(s) 636, 989, 2004
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BanI GGYRCC 2 cut(s) 1169, 1457
BanII GRGCYC 3 cut(s) 265, 504, 636
BauI CACGAG 2 cut(s) 988, 1791
BbsI GAAGAC 3 cut(s) 638, 1419, 2099
Bbv12I GWGCWC 2 cut(s) 989, 1305
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 5 cut(s) 778, 1334, 1463, 1640, 1643
BciT130I CCWGG 5 cut(s) 249, 637, 2141, 2146, 2346
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 1 cut(s) 1927
BcnI CCSGG 2 cut(s) 499, 1573
BcoDI GTCTC 4 cut(s) 368, 390, 728, 1983
BcuI ACTAGT 1 cut(s) 1540
BfaI CTAG 5 cut(s) 534, 1268, 1526, 1541, 2048
BfmI CTRYAG 3 cut(s) 405, 562, 1156
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 6 cut(s) 767, 1323, 1452, 1654, 1657, 2264
BlsI GCNGC 6 cut(s) 768, 1324, 1453, 1655, 1658, 2265
Bme1390I CCNGG 7 cut(s) 249, 499, 637, 1573, 2141, 2146, 2346
Bme18I GGWCC 3 cut(s) 292, 1505, 1843
BmeT110I CYCGRG 1 cut(s) 1982
BmgT120I GGNCC 7 cut(s) 7, 292, 632, 633, 1505, 1843, 2265
BmiI GGNNCC 6 cut(s) 262, 634, 1171, 1459, 1844, 2343
BmrFI CCNGG 7 cut(s) 249, 499, 637, 1573, 2141, 2146, 2346
BmsI GCATC 3 cut(s) 1242, 1268, 1668
BoxI GACNNNNGTC 1 cut(s) 1158
BpiI GAAGAC 3 cut(s) 638, 1419, 2099
BplI GAGNNNNNCTC 2 cut(s) 1785, 1817
BpmI CTGGAG 1 cut(s) 1804
Bpu10I CCTNAGC 2 cut(s) 588, 603
Bpu14I TTCGAA 3 cut(s) 206, 625, 671
BpuEI CTTGAG 2 cut(s) 1456, 1832
BpuMI CCSGG 2 cut(s) 499, 1573
Bsa29I ATCGAT 1 cut(s) 853
BsaAI YACGTR 2 cut(s) 729, 1965
BsaBI GATNNNNATC 2 cut(s) 1682, 2097
BsaJI CCNNGG 4 cut(s) 114, 635, 2139, 2370
BsaWI WCCGGW 2 cut(s) 125, 158
BsaXI ACNNNNNCTCC 6 cut(s) 67, 97, 605, 635, 1802, 1832
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 548, 641, 2145
Bse118I RCCGGY 2 cut(s) 1172, 1277
Bse1I ACTGG 5 cut(s) 382, 661, 1008, 1508, 2279
Bse3DI GCAATG 1 cut(s) 2328
Bse8I GATNNNNATC 2 cut(s) 1682, 2097
BseAI TCCGGA 1 cut(s) 158
BseBI CCWGG 5 cut(s) 249, 637, 2141, 2146, 2346
BseCI ATCGAT 1 cut(s) 853
BseDI CCNNGG 4 cut(s) 114, 635, 2139, 2370
BseGI GGATG 6 cut(s) 477, 488, 1257, 1683, 2086, 2380
BseJI GATNNNNATC 2 cut(s) 1682, 2097
BseLI CCNNNNNNNGG 4 cut(s) 248, 548, 641, 2145
BseMI GCAATG 1 cut(s) 2328
BseMII CTCAG 2 cut(s) 602, 1332
BseNI ACTGG 5 cut(s) 382, 661, 1008, 1508, 2279
BseSI GKGCMC 3 cut(s) 636, 989, 2004
BseXI GCAGC 5 cut(s) 778, 1334, 1463, 1640, 1643
BseYI CCCAGC 2 cut(s) 948, 1342
BsgI GTGCAG 2 cut(s) 87, 1773
BshFI GGCC 5 cut(s) 9, 634, 1520, 2144, 2266
BshNI GGYRCC 2 cut(s) 1169, 1457
BshVI ATCGAT 1 cut(s) 853
BsiHKAI GWGCWC 2 cut(s) 989, 1305
BsiHKCI CYCGRG 1 cut(s) 1982
BsiSI CCGG 6 cut(s) 126, 159, 498, 1173, 1278, 1572
BslFI GGGAC 3 cut(s) 1856, 2122, 2127
BslI CCNNNNNNNGG 4 cut(s) 248, 548, 641, 2145
BsmAI GTCTC 4 cut(s) 368, 390, 728, 1983
BsmFI GGGAC 3 cut(s) 1856, 2122, 2127
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 5 cut(s) 9, 634, 1520, 2144, 2266
BsoBI CYCGRG 1 cut(s) 1982
Bsp119I TTCGAA 3 cut(s) 206, 625, 671
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 6 cut(s) 265, 504, 636, 989, 1305, 2004
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
Bsp19I CCATGG 1 cut(s) 2370
BspACI CCGC 6 cut(s) 103, 410, 467, 793, 1310, 2263
BspANI GGCC 5 cut(s) 9, 634, 1520, 2144, 2266
BspCNI CTCAG 2 cut(s) 601, 1331
BspDI ATCGAT 1 cut(s) 853
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 6 cut(s) 262, 634, 1171, 1459, 1844, 2343
BspPI GGATC 4 cut(s) 347, 533, 616, 2180
BspT104I TTCGAA 3 cut(s) 206, 625, 671
BspT107I GGYRCC 2 cut(s) 1169, 1457
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 2328
BsrFI RCCGGY 2 cut(s) 1172, 1277
BsrI ACTGG 5 cut(s) 382, 661, 1008, 1508, 2279
BssAI RCCGGY 2 cut(s) 1172, 1277
BssECI CCNNGG 4 cut(s) 114, 635, 2139, 2370
BssMI GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
BssNAI GTATAC 2 cut(s) 558, 2234
BssSI CACGAG 2 cut(s) 988, 1791
BssT1I CCWWGG 2 cut(s) 114, 2370
Bst1107I GTATAC 2 cut(s) 558, 2234
Bst2BI CACGAG 2 cut(s) 988, 1791
Bst2UI CCWGG 5 cut(s) 249, 637, 2141, 2146, 2346
Bst4CI ACNGT 6 cut(s) 217, 319, 343, 1160, 1431, 2105
Bst6I CTCTTC 2 cut(s) 155, 1382
BstBAI YACGTR 2 cut(s) 729, 1965
BstBI TTCGAA 3 cut(s) 206, 625, 671
BstC8I GCNNGC 3 cut(s) 1257, 1456, 2255
BstDEI CTNAG 5 cut(s) 46, 588, 603, 1318, 2225
BstDSI CCRYGG 1 cut(s) 2370
BstF5I GGATG 6 cut(s) 477, 488, 1257, 1683, 2086, 2380
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 8 cut(s) 355, 541, 611, 853, 1403, 1930, 2095, 2188
BstMAI GTCTC 4 cut(s) 368, 390, 728, 1983
BstMBI GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
BstMWI GCNNNNNNNGC 6 cut(s) 799, 885, 1526, 1716, 1756, 2263
BstNI CCWGG 5 cut(s) 249, 637, 2141, 2146, 2346
BstPAI GACNNNNGTC 1 cut(s) 1158
BstSCI CCNGG 7 cut(s) 247, 497, 635, 1571, 2139, 2144, 2344
BstSFI CTRYAG 3 cut(s) 405, 562, 1156
BstSLI GKGCMC 3 cut(s) 636, 989, 2004
BstV1I GCAGC 5 cut(s) 778, 1334, 1463, 1640, 1643
BstV2I GAAGAC 3 cut(s) 638, 1419, 2099
BstX2I RGATCY 2 cut(s) 352, 2185
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 352, 2185
BstZ17I GTATAC 2 cut(s) 558, 2234
Bsu15I ATCGAT 1 cut(s) 853
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 5 cut(s) 9, 634, 1520, 2144, 2266
BsuTUI ATCGAT 1 cut(s) 853
BtgI CCRYGG 1 cut(s) 2370
BtsCI GGATG 6 cut(s) 477, 488, 1257, 1683, 2086, 2380
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 6 cut(s) 70, 315, 816, 1427, 2016, 2298
Cac8I GCNNGC 3 cut(s) 1257, 1456, 2255
CfoI GCGC 1 cut(s) 881
Cfr10I RCCGGY 2 cut(s) 1172, 1277
Cfr13I GGNCC 7 cut(s) 7, 292, 632, 633, 1505, 1843, 2265
ClaI ATCGAT 1 cut(s) 853
Csp6I GTAC 4 cut(s) 193, 285, 1987, 2222
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviQI GTAC 4 cut(s) 193, 285, 1987, 2222
DdeI CTNAG 5 cut(s) 46, 588, 603, 1318, 2225
DpnI GATC 8 cut(s) 354, 540, 610, 852, 1402, 1929, 2094, 2187
DpnII GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
DraI TTTAAA 1 cut(s) 1192
Eam1104I CTCTTC 2 cut(s) 155, 1382
EarI CTCTTC 2 cut(s) 155, 1382
Eco130I CCWWGG 2 cut(s) 114, 2370
Eco147I AGGCCT 1 cut(s) 2144
Eco24I GRGCYC 3 cut(s) 265, 504, 636
Eco32I GATATC 1 cut(s) 1207
Eco47I GGWCC 3 cut(s) 292, 1505, 1843
Eco57I CTGAAG 2 cut(s) 795, 1956
Eco88I CYCGRG 1 cut(s) 1982
EcoO109I RGGNCCY 3 cut(s) 7, 633, 1843
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 5 cut(s) 247, 635, 2139, 2144, 2344
EcoRV GATATC 1 cut(s) 1207
EcoT14I CCWWGG 2 cut(s) 114, 2370
EcoT38I GRGCYC 3 cut(s) 265, 504, 636
ErhI CCWWGG 2 cut(s) 114, 2370
FaqI GGGAC 3 cut(s) 1856, 2122, 2127
FauI CCCGC 3 cut(s) 110, 403, 460
FauNDI CATATG 1 cut(s) 2206
FbaI TGATCA 1 cut(s) 1927
FblI GTMKAC 2 cut(s) 557, 2233
Fnu4HI GCNGC 6 cut(s) 767, 1323, 1452, 1654, 1657, 2264
FokI GGATG 6 cut(s) 484, 495, 1264, 1690, 2073, 2387
FriOI GRGCYC 3 cut(s) 265, 504, 636
Fsp4HI GCNGC 6 cut(s) 767, 1323, 1452, 1654, 1657, 2264
FspBI CTAG 5 cut(s) 534, 1268, 1526, 1541, 2048
GlaI GCGC 1 cut(s) 880
GluI GCNGC 6 cut(s) 767, 1323, 1452, 1654, 1657, 2264
GsaI CCCAGC 2 cut(s) 952, 1346
GsuI CTGGAG 1 cut(s) 1804
HaeIII GGCC 5 cut(s) 9, 634, 1520, 2144, 2266
HapII CCGG 6 cut(s) 126, 159, 498, 1173, 1278, 1572
HhaI GCGC 1 cut(s) 881
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 2 cut(s) 1078, 1771
HindII GTYRAC 2 cut(s) 1078, 1771
HindIII AAGCTT 2 cut(s) 476, 1708
HinfI GANTC 8 cut(s) 147, 1557, 1568, 1633, 1767, 1817, 1830, 2201
HpaII CCGG 6 cut(s) 126, 159, 498, 1173, 1278, 1572
HphI GGTGA 5 cut(s) 91, 563, 900, 2087, 2390
Hpy166II GTNNAC 8 cut(s) 195, 558, 654, 987, 1078, 1513, 1771, 2234
Hpy188III TCNNGA 7 cut(s) 159, 514, 1398, 1821, 1849, 1982, 2090
Hpy8I GTNNAC 8 cut(s) 195, 558, 654, 987, 1078, 1513, 1771, 2234
HpyCH4III ACNGT 6 cut(s) 217, 319, 343, 1160, 1431, 2105
HpyCH4IV ACGT 2 cut(s) 728, 1964
HpyF10VI GCNNNNNNNGC 6 cut(s) 799, 885, 1526, 1716, 1756, 2263
HpyF3I CTNAG 5 cut(s) 46, 588, 603, 1318, 2225
HpySE526I ACGT 2 cut(s) 728, 1964
HspAI GCGC 1 cut(s) 879
Kpn2I TCCGGA 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 1927
Kzo9I GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
LmnI GCTCC 4 cut(s) 110, 260, 2318, 2341
Lsp1109I GCAGC 5 cut(s) 778, 1334, 1463, 1640, 1643
LweI GCATC 3 cut(s) 1242, 1268, 1668
MaeI CTAG 5 cut(s) 534, 1268, 1526, 1541, 2048
MaeII ACGT 2 cut(s) 728, 1964
MaeIII GTNAC 3 cut(s) 863, 917, 1603
MalI GATC 8 cut(s) 354, 540, 610, 852, 1402, 1929, 2094, 2187
MbiI CCGCTC 1 cut(s) 105
MboI GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
MfeI CAATTG 2 cut(s) 1446, 1992
MflI RGATCY 2 cut(s) 352, 2185
MhlI GDGCHC 6 cut(s) 265, 504, 636, 989, 1305, 2004
MlyI GAGTC 5 cut(s) 141, 1551, 1776, 1811, 2195
MmeI TCCRAC 2 cut(s) 110, 1044
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 1026
MseI TTAA 8 cut(s) 272, 573, 900, 969, 1191, 1593, 1641, 2306
MslI CAYNNNNRTG 1 cut(s) 2121
MspA1I CMGCKG 2 cut(s) 410, 769
MspI CCGG 6 cut(s) 126, 159, 498, 1173, 1278, 1572
MspR9I CCNGG 7 cut(s) 249, 499, 637, 1573, 2141, 2146, 2346
MunI CAATTG 2 cut(s) 1446, 1992
Mva1269I GAATGC 1 cut(s) 1106
MvaI CCWGG 5 cut(s) 249, 637, 2141, 2146, 2346
MwoI GCNNNNNNNGC 6 cut(s) 799, 885, 1526, 1716, 1756, 2263
NciI CCSGG 2 cut(s) 499, 1573
NcoI CCATGG 1 cut(s) 2370
NdeI CATATG 1 cut(s) 2206
NdeII GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
NlaIV GGNNCC 6 cut(s) 262, 634, 1171, 1459, 1844, 2343
NmuCI GTSAC 1 cut(s) 917
NspV TTCGAA 3 cut(s) 206, 625, 671
PceI AGGCCT 1 cut(s) 2144
PctI GAATGC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 3 cut(s) 1568, 1633, 1830
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 6 cut(s) 768, 1324, 1453, 1655, 1658, 2265
PleI GAGTC 5 cut(s) 141, 1551, 1775, 1811, 2195
PpsI GAGTC 5 cut(s) 141, 1551, 1775, 1811, 2195
Ppu21I YACGTR 2 cut(s) 729, 1965
PpuMI RGGWCCY 1 cut(s) 1843
PshAI GACNNNNGTC 1 cut(s) 1158
PsiI TTATAA 3 cut(s) 686, 1364, 1778
Psp5II RGGWCCY 1 cut(s) 1843
Psp6I CCWGG 5 cut(s) 247, 635, 2139, 2144, 2344
PspFI CCCAGC 2 cut(s) 948, 1342
PspGI CCWGG 5 cut(s) 247, 635, 2139, 2144, 2344
PspN4I GGNNCC 6 cut(s) 262, 634, 1171, 1459, 1844, 2343
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 7 cut(s) 7, 292, 632, 633, 1505, 1843, 2265
PspPPI RGGWCCY 1 cut(s) 1843
PsuI RGATCY 2 cut(s) 352, 2185
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 4 cut(s) 194, 286, 1988, 2223
RsaNI GTAC 4 cut(s) 193, 285, 1987, 2222
RseI CAYNNNNRTG 1 cut(s) 2121
SaqAI TTAA 8 cut(s) 272, 573, 900, 969, 1191, 1593, 1641, 2306
SatI GCNGC 6 cut(s) 767, 1323, 1452, 1654, 1657, 2264
Sau3AI GATC 8 cut(s) 352, 538, 608, 850, 1400, 1927, 2092, 2185
Sau96I GGNCC 7 cut(s) 7, 292, 632, 633, 1505, 1843, 2265
SchI GAGTC 5 cut(s) 141, 1551, 1776, 1811, 2195
ScrFI CCNGG 7 cut(s) 249, 499, 637, 1573, 2141, 2146, 2346
SduI GDGCHC 6 cut(s) 265, 504, 636, 989, 1305, 2004
SfaNI GCATC 3 cut(s) 1242, 1268, 1668
SfcI CTRYAG 3 cut(s) 405, 562, 1156
SfuI TTCGAA 3 cut(s) 206, 625, 671
SinI GGWCC 3 cut(s) 292, 1505, 1843
SmiMI CAYNNNNRTG 1 cut(s) 2121
SmlI CTYRAG 2 cut(s) 1471, 1847
SmoI CTYRAG 2 cut(s) 1471, 1847
SpeI ACTAGT 1 cut(s) 1540
SseBI AGGCCT 1 cut(s) 2144
SsiI CCGC 6 cut(s) 103, 410, 467, 793, 1310, 2263
SspMI CTAG 5 cut(s) 534, 1268, 1526, 1541, 2048
StuI AGGCCT 1 cut(s) 2144
StyD4I CCNGG 7 cut(s) 247, 497, 635, 1571, 2139, 2144, 2344
StyI CCWWGG 2 cut(s) 114, 2370
TaaI ACNGT 6 cut(s) 217, 319, 343, 1160, 1431, 2105
TaiI ACGT 2 cut(s) 731, 1967
TaqI TCGA 9 cut(s) 206, 625, 671, 853, 1403, 1728, 1869, 2271, 2446
TauI GCSGC 1 cut(s) 2266
TfiI GAWTC 3 cut(s) 1568, 1633, 1830
Tru1I TTAA 8 cut(s) 272, 573, 900, 969, 1191, 1593, 1641, 2306
Tru9I TTAA 8 cut(s) 272, 573, 900, 969, 1191, 1593, 1641, 2306
TscAI CASTG 6 cut(s) 70, 322, 823, 1434, 2016, 2298
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 5 cut(s) 766, 1322, 1451, 1653, 1656
Tsp45I GTSAC 1 cut(s) 917
TspRI CASTG 6 cut(s) 70, 322, 823, 1434, 2016, 2298
Van91I CCANNNNNTGG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 985
VpaK11BI GGWCC 3 cut(s) 292, 1505, 1843
XapI RAATTY 4 cut(s) 186, 382, 1035, 1914
XcmI CCANNNNNNNNNTGG 1 cut(s) 2434
XmiI GTMKAC 2 cut(s) 557, 2233
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 5 cut(s) 534, 1268, 1526, 1541, 2048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.