Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 705 |
| AccB1I | GGYRCC | 1 cut(s) | 1463 |
| AccI | GTMKAC | 1 cut(s) | 836 |
| AciI | CCGC | 4 cut(s) | 66, 625, 1771, 2276 |
| AclWI | GGATC | 3 cut(s) | 616, 1882, 2162 |
| AcsI | RAATTY | 6 cut(s) | 199, 620, 681, 1034, 1920, 2009 |
| AcuI | CTGAAG | 3 cut(s) | 369, 1248, 1962 |
| AdeI | CACNNNGTG | 1 cut(s) | 580 |
| AfaI | GTAC | 7 cut(s) | 194, 817, 1282, 1338, 1700, 1994, 2183 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 548, 641, 1018 |
| AflII | CTTAAG | 1 cut(s) | 1853 |
| AjiI | CACGTC | 1 cut(s) | 2123 |
| AjnI | CCWGG | 3 cut(s) | 466, 1478, 2326 |
| Alw26I | GTCTC | 6 cut(s) | 14, 549, 989, 1358, 1555, 1989 |
| AlwI | GGATC | 3 cut(s) | 616, 1882, 2162 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1334 |
| AoxI | GGCC | 1 cut(s) | 499 |
| ApeKI | GCWGC | 8 cut(s) | 68, 83, 478, 1328, 1659, 1662, 1753, 2067 |
| ApoI | RAATTY | 6 cut(s) | 199, 620, 681, 1034, 1920, 2009 |
| AseI | ATTAAT | 1 cut(s) | 1310 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 620, 1399 |
| AspS9I | GGNCC | 4 cut(s) | 500, 632, 1511, 1849 |
| AsuHPI | GGTGA | 2 cut(s) | 566, 794 |
| AsuII | TTCGAA | 1 cut(s) | 1926 |
| AvaII | GGWCC | 3 cut(s) | 632, 1511, 1849 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 102, 135 |
| BanI | GGYRCC | 1 cut(s) | 1463 |
| BauI | CACGAG | 1 cut(s) | 1797 |
| BbsI | GAAGAC | 4 cut(s) | 318, 638, 653, 1425 |
| BbvCI | CCTCAGC | 1 cut(s) | 588 |
| BbvI | GCAGC | 8 cut(s) | 70, 80, 465, 1340, 1646, 1649, 1740, 2079 |
| BccI | CCATC | 2 cut(s) | 457, 1824 |
| BceAI | ACGGC | 2 cut(s) | 809, 1347 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 833, 867 |
| BciT130I | CCWGG | 3 cut(s) | 468, 1480, 2328 |
| BclI | TGATCA | 2 cut(s) | 1234, 1735 |
| BcoDI | GTCTC | 6 cut(s) | 14, 549, 989, 1358, 1555, 1989 |
| BfaI | CTAG | 9 cut(s) | 80, 168, 182, 330, 534, 797, 1271, 1874, 2427 |
| BfrI | CTTAAG | 1 cut(s) | 1853 |
| BisI | GCNGC | 8 cut(s) | 69, 84, 479, 1329, 1660, 1663, 1754, 2068 |
| BlsI | GCNGC | 8 cut(s) | 70, 85, 480, 1330, 1661, 1664, 1755, 2069 |
| BmcAI | AGTACT | 2 cut(s) | 817, 2183 |
| Bme1390I | CCNGG | 3 cut(s) | 468, 1480, 2328 |
| Bme18I | GGWCC | 3 cut(s) | 632, 1511, 1849 |
| BmgBI | CACGTC | 1 cut(s) | 2123 |
| BmgT120I | GGNCC | 4 cut(s) | 500, 632, 1511, 1849 |
| BmiI | GGNNCC | 4 cut(s) | 262, 1465, 1850, 2325 |
| BmrFI | CCNGG | 3 cut(s) | 468, 1480, 2328 |
| BmrI | ACTGGG | 1 cut(s) | 152 |
| BmsI | GCATC | 5 cut(s) | 777, 845, 1245, 1674, 2201 |
| BmuI | ACTGGG | 1 cut(s) | 152 |
| BpiI | GAAGAC | 4 cut(s) | 318, 638, 653, 1425 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1791, 1823 |
| Bpu10I | CCTNAGC | 1 cut(s) | 588 |
| Bpu14I | TTCGAA | 1 cut(s) | 1926 |
| BpuEI | CTTGAG | 2 cut(s) | 588, 1810 |
| Bsa29I | ATCGAT | 2 cut(s) | 570, 853 |
| BsaAI | YACGTR | 1 cut(s) | 2191 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1688 |
| BsaI | GGTCTC | 3 cut(s) | 14, 989, 1358 |
| BsaJI | CCNNGG | 2 cut(s) | 114, 635 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 548, 641, 1018 |
| Bse1I | ACTGG | 6 cut(s) | 147, 382, 661, 1284, 1514, 1766 |
| Bse3DI | GCAATG | 2 cut(s) | 86, 2306 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1688 |
| BseBI | CCWGG | 3 cut(s) | 468, 1480, 2328 |
| BseCI | ATCGAT | 2 cut(s) | 570, 853 |
| BseDI | CCNNGG | 2 cut(s) | 114, 635 |
| BseGI | GGATG | 4 cut(s) | 1260, 1689, 2032, 2092 |
| BseJI | GATNNNNATC | 1 cut(s) | 1688 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 548, 641, 1018 |
| BseMI | GCAATG | 2 cut(s) | 86, 2306 |
| BseMII | CTCAG | 3 cut(s) | 602, 1329, 1980 |
| BseNI | ACTGG | 6 cut(s) | 147, 382, 661, 1284, 1514, 1766 |
| BseRI | GAGGAG | 1 cut(s) | 2391 |
| BseXI | GCAGC | 8 cut(s) | 70, 80, 465, 1340, 1646, 1649, 1740, 2079 |
| BshFI | GGCC | 1 cut(s) | 501 |
| BshNI | GGYRCC | 1 cut(s) | 1463 |
| BshVI | ATCGAT | 2 cut(s) | 570, 853 |
| BslFI | GGGAC | 3 cut(s) | 159, 1862, 2133 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 548, 641, 1018 |
| BsmAI | GTCTC | 6 cut(s) | 14, 549, 989, 1358, 1555, 1989 |
| BsmBI | CGTCTC | 1 cut(s) | 549 |
| BsmFI | GGGAC | 3 cut(s) | 159, 1862, 2133 |
| BsmI | GAATGC | 1 cut(s) | 2214 |
| BsnI | GGCC | 1 cut(s) | 501 |
| Bso31I | GGTCTC | 3 cut(s) | 14, 989, 1358 |
| Bsp119I | TTCGAA | 1 cut(s) | 1926 |
| BspACI | CCGC | 4 cut(s) | 66, 625, 1771, 2276 |
| BspANI | GGCC | 1 cut(s) | 501 |
| BspCNI | CTCAG | 3 cut(s) | 601, 1328, 1981 |
| BspDI | ATCGAT | 2 cut(s) | 570, 853 |
| BspLI | GGNNCC | 4 cut(s) | 262, 1465, 1850, 2325 |
| BspPI | GGATC | 3 cut(s) | 616, 1882, 2162 |
| BspT104I | TTCGAA | 1 cut(s) | 1926 |
| BspT107I | GGYRCC | 1 cut(s) | 1463 |
| BspTI | CTTAAG | 1 cut(s) | 1853 |
| BspTNI | GGTCTC | 3 cut(s) | 14, 989, 1358 |
| BsrDI | GCAATG | 2 cut(s) | 86, 2306 |
| BsrI | ACTGG | 6 cut(s) | 147, 382, 661, 1284, 1514, 1766 |
| BssECI | CCNNGG | 2 cut(s) | 114, 635 |
| BssNAI | GTATAC | 1 cut(s) | 837 |
| BssSI | CACGAG | 1 cut(s) | 1797 |
| BssT1I | CCWWGG | 2 cut(s) | 114, 635 |
| Bst1107I | GTATAC | 1 cut(s) | 837 |
| Bst2BI | CACGAG | 1 cut(s) | 1797 |
| Bst2UI | CCWGG | 3 cut(s) | 468, 1480, 2328 |
| Bst4CI | ACNGT | 2 cut(s) | 1880, 2136 |
| Bst6I | CTCTTC | 1 cut(s) | 1587 |
| BstAFI | CTTAAG | 1 cut(s) | 1853 |
| BstBAI | YACGTR | 1 cut(s) | 2191 |
| BstBI | TTCGAA | 1 cut(s) | 1926 |
| BstC8I | GCNNGC | 1 cut(s) | 1260 |
| BstDEI | CTNAG | 5 cut(s) | 588, 1315, 1532, 1989, 2207 |
| BstF5I | GGATG | 4 cut(s) | 1260, 1689, 2032, 2092 |
| BstMAI | GTCTC | 6 cut(s) | 14, 549, 989, 1358, 1555, 1989 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1762 |
| BstNI | CCWGG | 3 cut(s) | 468, 1480, 2328 |
| BstNSI | RCATGY | 1 cut(s) | 1705 |
| BstSCI | CCNGG | 3 cut(s) | 466, 1478, 2326 |
| BstV1I | GCAGC | 8 cut(s) | 70, 80, 465, 1340, 1646, 1649, 1740, 2079 |
| BstV2I | GAAGAC | 4 cut(s) | 318, 638, 653, 1425 |
| BstX2I | RGATCY | 1 cut(s) | 2167 |
| BstYI | RGATCY | 1 cut(s) | 2167 |
| BstZ17I | GTATAC | 1 cut(s) | 837 |
| Bsu15I | ATCGAT | 2 cut(s) | 570, 853 |
| BsuRI | GGCC | 1 cut(s) | 501 |
| BsuTUI | ATCGAT | 2 cut(s) | 570, 853 |
| BtrI | CACGTC | 1 cut(s) | 2123 |
| BtsCI | GGATG | 4 cut(s) | 1260, 1689, 2032, 2092 |
| BtsI | GCAGTG | 2 cut(s) | 458, 634 |
| BtsIMutI | CAGTG | 5 cut(s) | 458, 634, 1080, 1323, 2022 |
| Cac8I | GCNNGC | 1 cut(s) | 1260 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1334 |
| Cfr13I | GGNCC | 4 cut(s) | 500, 632, 1511, 1849 |
| ClaI | ATCGAT | 2 cut(s) | 570, 853 |
| Csp6I | GTAC | 7 cut(s) | 193, 816, 1281, 1337, 1699, 1993, 2182 |
| CviAII | CATG | 9 cut(s) | 174, 265, 1087, 1702, 1786, 2003, 2127, 2172, 2284 |
| CviQI | GTAC | 7 cut(s) | 193, 816, 1281, 1337, 1699, 1993, 2182 |
| DdeI | CTNAG | 5 cut(s) | 588, 1315, 1532, 1989, 2207 |
| DraIII | CACNNNGTG | 1 cut(s) | 580 |
| Eam1104I | CTCTTC | 1 cut(s) | 1587 |
| EarI | CTCTTC | 1 cut(s) | 1587 |
| Eco130I | CCWWGG | 2 cut(s) | 114, 635 |
| Eco31I | GGTCTC | 3 cut(s) | 14, 989, 1358 |
| Eco32I | GATATC | 1 cut(s) | 1323 |
| Eco47I | GGWCC | 3 cut(s) | 632, 1511, 1849 |
| Eco57I | CTGAAG | 3 cut(s) | 369, 1248, 1962 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1849 |
| EcoRI | GAATTC | 1 cut(s) | 620 |
| EcoRII | CCWGG | 3 cut(s) | 466, 1478, 2326 |
| EcoRV | GATATC | 1 cut(s) | 1323 |
| EcoT14I | CCWWGG | 2 cut(s) | 114, 635 |
| EcoT22I | ATGCAT | 1 cut(s) | 2216 |
| ErhI | CCWWGG | 2 cut(s) | 114, 635 |
| Esp3I | CGTCTC | 1 cut(s) | 549 |
| FaeI | CATG | 9 cut(s) | 177, 268, 1090, 1705, 1789, 2006, 2130, 2175, 2287 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 28 |
| FaqI | GGGAC | 3 cut(s) | 159, 1862, 2133 |
| FatI | CATG | 9 cut(s) | 173, 264, 1086, 1701, 1785, 2002, 2126, 2171, 2283 |
| FauNDI | CATATG | 2 cut(s) | 890, 1972 |
| FbaI | TGATCA | 2 cut(s) | 1234, 1735 |
| FblI | GTMKAC | 1 cut(s) | 836 |
| Fnu4HI | GCNGC | 8 cut(s) | 69, 84, 479, 1329, 1660, 1663, 1754, 2068 |
| FokI | GGATG | 4 cut(s) | 1267, 1696, 2039, 2079 |
| Fsp4HI | GCNGC | 8 cut(s) | 69, 84, 479, 1329, 1660, 1663, 1754, 2068 |
| FspBI | CTAG | 9 cut(s) | 80, 168, 182, 330, 534, 797, 1271, 1874, 2427 |
| GluI | GCNGC | 8 cut(s) | 69, 84, 479, 1329, 1660, 1663, 1754, 2068 |
| HaeIII | GGCC | 1 cut(s) | 501 |
| Hin1II | CATG | 9 cut(s) | 177, 268, 1090, 1705, 1789, 2006, 2130, 2175, 2287 |
| HindIII | AAGCTT | 2 cut(s) | 133, 1714 |
| HinfI | GANTC | 7 cut(s) | 55, 251, 443, 1639, 1823, 1836, 2397 |
| HphI | GGTGA | 2 cut(s) | 566, 794 |
| Hpy166II | GTNNAC | 7 cut(s) | 63, 632, 837, 1078, 1519, 1995, 2194 |
| Hpy8I | GTNNAC | 7 cut(s) | 63, 632, 837, 1078, 1519, 1995, 2194 |
| HpyAV | CCTTC | 7 cut(s) | 345, 1009, 1016, 1285, 1664, 1937, 2291 |
| HpyCH4III | ACNGT | 2 cut(s) | 1880, 2136 |
| HpyCH4IV | ACGT | 2 cut(s) | 2122, 2190 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1762 |
| HpyF3I | CTNAG | 5 cut(s) | 588, 1315, 1532, 1989, 2207 |
| HpySE526I | ACGT | 2 cut(s) | 2122, 2190 |
| Hsp92II | CATG | 9 cut(s) | 177, 268, 1090, 1705, 1789, 2006, 2130, 2175, 2287 |
| Ksp22I | TGATCA | 2 cut(s) | 1234, 1735 |
| LmnI | GCTCC | 6 cut(s) | 260, 1195, 2300, 2323, 2354, 2378 |
| Lsp1109I | GCAGC | 8 cut(s) | 70, 80, 465, 1340, 1646, 1649, 1740, 2079 |
| LweI | GCATC | 5 cut(s) | 777, 845, 1245, 1674, 2201 |
| MaeI | CTAG | 9 cut(s) | 80, 168, 182, 330, 534, 797, 1271, 1874, 2427 |
| MaeII | ACGT | 2 cut(s) | 2122, 2190 |
| MaeIII | GTNAC | 4 cut(s) | 406, 536, 1609, 1880 |
| MfeI | CAATTG | 1 cut(s) | 1452 |
| MflI | RGATCY | 1 cut(s) | 2167 |
| MlyI | GAGTC | 2 cut(s) | 1817, 2391 |
| MmeI | TCCRAC | 3 cut(s) | 1044, 1654, 1926 |
| Mph1103I | ATGCAT | 1 cut(s) | 2216 |
| MroXI | GAANNNNTTC | 2 cut(s) | 620, 1399 |
| MseI | TTAA | 9 cut(s) | 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1784, 2219 |
| MspA1I | CMGCKG | 2 cut(s) | 71, 2276 |
| MspCI | CTTAAG | 1 cut(s) | 1853 |
| MspR9I | CCNGG | 3 cut(s) | 468, 1480, 2328 |
| MunI | CAATTG | 1 cut(s) | 1452 |
| Mva1269I | GAATGC | 1 cut(s) | 2214 |
| MvaI | CCWGG | 3 cut(s) | 468, 1480, 2328 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1762 |
| NdeI | CATATG | 2 cut(s) | 890, 1972 |
| NlaIII | CATG | 9 cut(s) | 177, 268, 1090, 1705, 1789, 2006, 2130, 2175, 2287 |
| NlaIV | GGNNCC | 4 cut(s) | 262, 1465, 1850, 2325 |
| NmuCI | GTSAC | 3 cut(s) | 406, 536, 1880 |
| NsiI | ATGCAT | 1 cut(s) | 2216 |
| NspI | RCATGY | 1 cut(s) | 1705 |
| NspV | TTCGAA | 1 cut(s) | 1926 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2196 |
| PctI | GAATGC | 1 cut(s) | 2214 |
| PdmI | GAANNNNTTC | 2 cut(s) | 620, 1399 |
| PfeI | GAWTC | 5 cut(s) | 55, 251, 443, 1639, 1836 |
| PflFI | GACNNNGTC | 1 cut(s) | 1584 |
| PkrI | GCNGC | 8 cut(s) | 70, 85, 480, 1330, 1661, 1664, 1755, 2069 |
| PleI | GAGTC | 2 cut(s) | 1817, 2391 |
| PpsI | GAGTC | 2 cut(s) | 1817, 2391 |
| Ppu21I | YACGTR | 1 cut(s) | 2191 |
| PpuMI | RGGWCCY | 1 cut(s) | 1849 |
| PshBI | ATTAAT | 1 cut(s) | 1310 |
| PsiI | TTATAA | 1 cut(s) | 705 |
| Psp5II | RGGWCCY | 1 cut(s) | 1849 |
| Psp6I | CCWGG | 3 cut(s) | 466, 1478, 2326 |
| PspGI | CCWGG | 3 cut(s) | 466, 1478, 2326 |
| PspN4I | GGNNCC | 4 cut(s) | 262, 1465, 1850, 2325 |
| PspPI | GGNCC | 4 cut(s) | 500, 632, 1511, 1849 |
| PspPPI | RGGWCCY | 1 cut(s) | 1849 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1334 |
| PsuI | RGATCY | 1 cut(s) | 2167 |
| PsyI | GACNNNGTC | 1 cut(s) | 1584 |
| PvuII | CAGCTG | 1 cut(s) | 71 |
| RsaI | GTAC | 7 cut(s) | 194, 817, 1282, 1338, 1700, 1994, 2183 |
| RsaNI | GTAC | 7 cut(s) | 193, 816, 1281, 1337, 1699, 1993, 2182 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1784, 2219 |
| SaqAI | TTAA | 9 cut(s) | 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369 |
| SatI | GCNGC | 8 cut(s) | 69, 84, 479, 1329, 1660, 1663, 1754, 2068 |
| Sau96I | GGNCC | 4 cut(s) | 500, 632, 1511, 1849 |
| ScaI | AGTACT | 2 cut(s) | 817, 2183 |
| SchI | GAGTC | 2 cut(s) | 1817, 2391 |
| ScrFI | CCNGG | 3 cut(s) | 468, 1480, 2328 |
| SfaNI | GCATC | 5 cut(s) | 777, 845, 1245, 1674, 2201 |
| SfuI | TTCGAA | 1 cut(s) | 1926 |
| SinI | GGWCC | 3 cut(s) | 632, 1511, 1849 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1784, 2219 |
| SmlI | CTYRAG | 3 cut(s) | 603, 1825, 1853 |
| SmoI | CTYRAG | 3 cut(s) | 603, 1825, 1853 |
| SsiI | CCGC | 4 cut(s) | 66, 625, 1771, 2276 |
| SspMI | CTAG | 9 cut(s) | 80, 168, 182, 330, 534, 797, 1271, 1874, 2427 |
| StyD4I | CCNGG | 3 cut(s) | 466, 1478, 2326 |
| StyI | CCWWGG | 2 cut(s) | 114, 635 |
| TaaI | ACNGT | 2 cut(s) | 1880, 2136 |
| TaiI | ACGT | 2 cut(s) | 2125, 2193 |
| TaqI | TCGA | 4 cut(s) | 570, 853, 1926, 2414 |
| TatI | WGTACW | 5 cut(s) | 815, 1336, 1698, 1992, 2181 |
| TfiI | GAWTC | 5 cut(s) | 55, 251, 443, 1639, 1836 |
| Tru1I | TTAA | 9 cut(s) | 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369 |
| Tru9I | TTAA | 9 cut(s) | 764, 900, 969, 1310, 1647, 1758, 1832, 1854, 2369 |
| TscAI | CASTG | 5 cut(s) | 465, 634, 1080, 1323, 2022 |
| TseFI | GTSAC | 3 cut(s) | 406, 536, 1880 |
| TseI | GCWGC | 8 cut(s) | 68, 83, 478, 1328, 1659, 1662, 1753, 2067 |
| Tsp45I | GTSAC | 3 cut(s) | 406, 536, 1880 |
| TspRI | CASTG | 5 cut(s) | 465, 634, 1080, 1323, 2022 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1584 |
| Vha464I | CTTAAG | 1 cut(s) | 1853 |
| VpaK11BI | GGWCC | 3 cut(s) | 632, 1511, 1849 |
| VspI | ATTAAT | 1 cut(s) | 1310 |
| XapI | RAATTY | 6 cut(s) | 199, 620, 681, 1034, 1920, 2009 |
| XbaI | TCTAGA | 1 cut(s) | 167 |
| XceI | RCATGY | 1 cut(s) | 1705 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1714 |
| XmiI | GTMKAC | 1 cut(s) | 836 |
| XmnI | GAANNNNTTC | 2 cut(s) | 620, 1399 |
| XspI | CTAG | 9 cut(s) | 80, 168, 182, 330, 534, 797, 1271, 1874, 2427 |
| ZrmI | AGTACT | 2 cut(s) | 817, 2183 |
| Zsp2I | ATGCAT | 1 cut(s) | 2216 |