Prupe.4G193300_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001151
Physical Location & Seq
Forward (+)
11640072 .. 11643903
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G193300.2

Sequence Viewer

Length: 2454 bp
ATGAAGGTCCTTGCATTAATCCTCTCTACCTATCTACTCCATTTTTTAGTCTTACAATTGTCCGGAGCAGCTGATACAATCTCTGCTGCACAATCCATCACCAGCACTAATACCTTGGTTTCTTCCGGCCAAAGCTTCGAATTAGGACTCTTCTCCCCGGGCAATTCAGAGGCCTGGTATTTAGGAATATGGTACAAGAATTTTCCAACTATTGTTGTATGGGTTGCCAACAGAGAAAATCCAGTTGCAGATTCACATGGATCCTTGAAATTATCCAAAAATGGAAGTCTTGTCCTTTTAGATCAAATGAACAATACTATTTGGTCTTCCACTTCTTCCCAAGTAGCAGAAGACCCTGTTGCACAACTTTTGGAGAATGGAAACCTGGTTGTCAGAGAAAAGGATACAACAGATTCAGAAAGCTACATATGGGAAAGTTTCAATTTGCCATCAGACACTCTGCTACCAGAAATGAAGGTTGGATGGGACTTCAGAACAGGCGTCAACAGATTCTTGACTTCTTGGAAAAATGCTAGTGACCCATCTCTTGGAGAATATACCTATGGGATTGATAATCTGATGTTGCCTCAGCTTGTTGTTGCCAAAGGATCAAAGAAATTGTTCCGTACCGGGCCTTGGAATGGTGTTCAATTTTCTGGTACTCCTGATTCTGGGAACAAACGAATTGTGAAACCAATCTATGTGTATGATACCAATGGGTTCTATTATATGTATGAGGCTACTGAGAGTTCTATTCTAACAAGAGTGAAGCTGTCTGAAACTGGTTTGGCCCAGCGGCTAGTGCTGAATGAAGGAAGCACTGAATGGGCTGTTATGTATACTTTGCTGAATGACAGGTGTGACAATTACAGGGAGTGTGGAGCAAATGGAATTTGCAGAACTTCCAAGTCTCCAAGTTGTGAGTGCCTGCAGGGGTTTGTACCAAAATCGCAAAACGAATGGGATGTGCTTAACTGGGAAAGTGGGTGCATTAGGCAGACACCACTGGATTGCCAGAAGGGAGAAGGGTTTTTGAAAGTTCGAAACGTGAAATTGCCAGACCTGTTGGAGTTTTGGGCAAACACAAAAATGAGTGTGCAGGAATGTGAGGCAGAGTGCTTGAGGAATTGTTCTTGTGTAGCTTATGCTAGTTCAGATATCCGTAACGGAGGAAGTGGCTGTCTGATGTGGTTTGGCGACCTAATTGATATGCGAGAGTTCCTTGAAGCAGATGTTGAGCAAGATATACACATTAGGATGCCATTTTCGGAACTAGAATCTTTAGGTGGTACCGGCAAGAAGGACAAAAGGGTTATACTTATCTCGGTCATATCAGCAGTTTCTGTGCTTCCACTTCTGGCTTTGTTATGCTGGTGTATACTTCTGAAGAAGAGAGGAAGGAATGTATCCACATCTACAGGCTCAAGATCAATTAAGGAGGACTGGGAGTTGCCACTGTTTGATTTTAAGACAATTGCAACCGCCACCAATAATTTCTCTCACACAAACAAACTAGGAGAGGGAGGTTTCGGTCCAGTTTACAAGGCCAACCTAACCCGAGAAGAATTCATAGCTGTAAAGAGACTCTCAAAAGATTCTGGGCAAGGTATTGAAGAGTTTAAGAATGAAGTTACAATGATAGCCAACCTCCAACACTGGAATCTTGTTAAACTTTTGGGCTGCTGCATTGAAAGAGAAGAAAGGATGCTTATCTATGAGTACATGCCCAACAAAAGCTTGGACTGCTTTATTTTCGATCAAAATAGAAAGGTTTTTCTGAATTGGCAAAAGCGATTGAACATTATCATGGGAATTGCTCGAGGACTTCTCTACCTCCACCAAGACTCCAGATTAAAAATCATTCATAGAGACCTTAAGAGTAGCAACATCTTACTTGATGATGAGCTGAACCCCAAAATATCTGACTTTGGCATAGCAAGGATTTTTGGACGCAATCAAACCGAAGCAAAAACAAAACGAGTAATTGGGACATATGGATATATGTCTCCTGAGTATGCAATTGATGGAAAGTTTTCAGAGAAATCCGATGTGTTCAGTTTTGGAGTGCTTTTGTTAGAGATAGTAAGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGATCACCATCACACTCTTCTGGGACATGCATGGTTACTGTGGAATGAAAATAAGGGTTTGGAGCTAATAGATCCTTGCTTGGGGTACTCATATGTTGAGTTTGAGGTGCTGAGGTGCATTCAGGTAGGTCTATTGTGTGTTCAAGCGCTTCCAAAAGACAGACCAGTAATGTCATCAGTGGTTGTCATGTTAAGCAATGAAGGAGTAACATTACCTCAACCAAAGGAGCCAGGATTCTTTACAGGAAGAAGTTCCCTGGACGATACCATCATCGACAAAGGTAGAGGTAGCCAGACAGGAAGCAGTATTACCATTTCGACAGTGGAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

818

Amino Acids

91.73

Weight (kDa)

6.1

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1289
AccB1I GGYRCC 1 cut(s) 1289
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 2 cut(s) 839, 1378
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 2 cut(s) 796, 1482
AclWI GGATC 4 cut(s) 255, 268, 616, 2183
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 3 cut(s) 199, 891, 1565
AcuI CTGAAG 2 cut(s) 475, 1406
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 7 cut(s) 194, 628, 661, 942, 1291, 1721, 2204
AfeI AGCGCT 1 cut(s) 2265
AfiI CCNNNNNNNGG 5 cut(s) 548, 636, 641, 671, 2198
AflII CTTAAG 1 cut(s) 1874
AgsI TTSAA 9 cut(s) 268, 442, 650, 1036, 1226, 1613, 1691, 1798, 2261
AjnI CCWGG 4 cut(s) 173, 384, 2347, 2373
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
Alw26I GTCTC 4 cut(s) 915, 1576, 1863, 2010
AlwI GGATC 4 cut(s) 255, 268, 616, 2183
AlwNI CAGNNNCTG 1 cut(s) 1343
Ama87I CYCGRG 3 cut(s) 157, 1557, 1818
Aor13HI TCCGGA 1 cut(s) 62
Aor51HI AGCGCT 1 cut(s) 2265
AoxI GGCC 5 cut(s) 127, 171, 632, 789, 1545
ApeKI GCWGC 4 cut(s) 68, 86, 1680, 1683
ApoI RAATTY 3 cut(s) 199, 891, 1565
AseI ATTAAT 1 cut(s) 17
Asp700I GAANNNNTTC 3 cut(s) 620, 2032, 2368
Asp718I GGTACC 1 cut(s) 1289
AspLEI GCGC 1 cut(s) 2266
AspS9I GGNCC 4 cut(s) 7, 632, 790, 1532
AsuC2I CCSGG 3 cut(s) 158, 159, 631
AsuHPI GGTGA 2 cut(s) 91, 2114
AsuII TTCGAA 2 cut(s) 138, 1042
AvaI CYCGRG 3 cut(s) 157, 1557, 1818
AvaII GGWCC 2 cut(s) 7, 1532
BamHI GGATCC 1 cut(s) 260
BanI GGYRCC 1 cut(s) 1289
BarI GAAGNNNNNNTAC 2 cut(s) 2410, 2442
BbsI GAAGAC 2 cut(s) 318, 357
BbvCI CCTCAGC 2 cut(s) 588, 2228
BbvI GCAGC 4 cut(s) 73, 80, 1667, 1670
BccI CCATC 7 cut(s) 104, 457, 477, 550, 2018, 2133, 2393
BciT130I CCWGG 4 cut(s) 175, 386, 2349, 2375
BciVI GTATCC 2 cut(s) 397, 1417
BcnI CCSGG 3 cut(s) 158, 159, 631
BcoDI GTCTC 4 cut(s) 915, 1576, 1863, 2010
BfaI CTAG 6 cut(s) 534, 800, 1149, 1274, 1514, 2448
BfmI CTRYAG 2 cut(s) 929, 1416
BfoI RGCGCY 1 cut(s) 2267
BfrI CTTAAG 1 cut(s) 1874
BfuI GTATCC 2 cut(s) 397, 1417
BisI GCNGC 5 cut(s) 69, 87, 797, 1681, 1684
BlsI GCNGC 5 cut(s) 70, 88, 798, 1682, 1685
Bme1390I CCNGG 7 cut(s) 158, 159, 175, 386, 631, 2349, 2375
Bme18I GGWCC 2 cut(s) 7, 1532
BmeT110I CYCGRG 3 cut(s) 157, 1557, 1818
BmgT120I GGNCC 4 cut(s) 7, 632, 790, 1532
BmiI GGNNCC 3 cut(s) 262, 1291, 2346
BmrFI CCNGG 7 cut(s) 158, 159, 175, 386, 631, 2349, 2375
BmrI ACTGGG 2 cut(s) 985, 1453
BmsI GCATC 2 cut(s) 1248, 1695
BmuI ACTGGG 2 cut(s) 985, 1453
BpiI GAAGAC 2 cut(s) 318, 357
BplI GAGNNNNNCTC 2 cut(s) 1812, 1844
BpmI CTGGAG 1 cut(s) 1831
Bpu10I CCTNAGC 2 cut(s) 588, 2228
Bpu14I TTCGAA 2 cut(s) 138, 1042
BpuEI CTTGAG 2 cut(s) 1141, 1408
BpuMI CCSGG 3 cut(s) 158, 159, 631
BsaBI GATNNNNATC 2 cut(s) 1709, 2124
BsaHI GRCGYC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 1863
BsaJI CCNNGG 5 cut(s) 114, 156, 157, 635, 2373
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 1829, 1859
Bsc4I CCNNNNNNNGG 5 cut(s) 548, 636, 641, 671, 2198
Bse118I RCCGGY 1 cut(s) 1292
Bse1I ACTGG 8 cut(s) 242, 787, 980, 1011, 1448, 1535, 1661, 2282
Bse3DI GCAATG 1 cut(s) 2320
Bse8I GATNNNNATC 2 cut(s) 1709, 2124
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 4 cut(s) 175, 386, 2349, 2375
BseDI CCNNGG 5 cut(s) 114, 156, 157, 635, 2373
BseGI GGATG 5 cut(s) 488, 970, 1263, 1710, 2113
BseJI GATNNNNATC 2 cut(s) 1709, 2124
BseLI CCNNNNNNNGG 5 cut(s) 548, 636, 641, 671, 2198
BseMI GCAATG 1 cut(s) 2320
BseMII CTCAG 4 cut(s) 602, 735, 2001, 2219
BseNI ACTGG 8 cut(s) 242, 787, 980, 1011, 1448, 1535, 1661, 2282
BseXI GCAGC 4 cut(s) 73, 80, 1667, 1670
BseYI CCCAGC 1 cut(s) 792
BsgI GTGCAG 2 cut(s) 72, 1118
BshFI GGCC 5 cut(s) 129, 173, 634, 791, 1547
BshNI GGYRCC 1 cut(s) 1289
BsiHKCI CYCGRG 3 cut(s) 157, 1557, 1818
BsiSI CCGG 5 cut(s) 63, 126, 158, 630, 1293
BslFI GGGAC 3 cut(s) 500, 2002, 2154
BslI CCNNNNNNNGG 5 cut(s) 548, 636, 641, 671, 2198
BsmAI GTCTC 4 cut(s) 915, 1576, 1863, 2010
BsmFI GGGAC 3 cut(s) 500, 2002, 2154
BsmI GAATGC 1 cut(s) 2235
BsnI GGCC 5 cut(s) 129, 173, 634, 791, 1547
Bso31I GGTCTC 1 cut(s) 1863
BsoBI CYCGRG 3 cut(s) 157, 1557, 1818
Bsp119I TTCGAA 2 cut(s) 138, 1042
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
BspACI CCGC 2 cut(s) 796, 1482
BspANI GGCC 5 cut(s) 129, 173, 634, 791, 1547
BspCNI CTCAG 4 cut(s) 601, 736, 2002, 2220
BspEI TCCGGA 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 262, 1291, 2346
BspMAI CTGCAG 1 cut(s) 933
BspPI GGATC 4 cut(s) 255, 268, 616, 2183
BspT104I TTCGAA 2 cut(s) 138, 1042
BspT107I GGYRCC 1 cut(s) 1289
BspTI CTTAAG 1 cut(s) 1874
BspTNI GGTCTC 1 cut(s) 1863
BsrDI GCAATG 1 cut(s) 2320
BsrFI RCCGGY 1 cut(s) 1292
BsrI ACTGG 8 cut(s) 242, 787, 980, 1011, 1448, 1535, 1661, 2282
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 5 cut(s) 114, 156, 157, 635, 2373
BssMI GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
BssNAI GTATAC 2 cut(s) 840, 1379
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 114, 635
Bst1107I GTATAC 2 cut(s) 840, 1379
Bst2UI CCWGG 4 cut(s) 175, 386, 2349, 2375
Bst4CI ACNGT 3 cut(s) 1458, 2157, 2440
Bst6I CTCTTC 4 cut(s) 155, 1385, 1608, 2139
BstACI GRCGYC 1 cut(s) 501
BstAFI CTTAAG 1 cut(s) 1874
BstBI TTCGAA 2 cut(s) 138, 1042
BstC8I GCNNGC 1 cut(s) 929
BstDEI CTNAG 4 cut(s) 588, 744, 2010, 2228
BstF5I GGATG 5 cut(s) 488, 970, 1263, 1710, 2113
BstH2I RGCGCY 1 cut(s) 2267
BstHHI GCGC 1 cut(s) 2266
BstKTI GATC 7 cut(s) 263, 304, 611, 1430, 1759, 2122, 2191
BstMAI GTCTC 4 cut(s) 915, 1576, 1863, 2010
BstMBI GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
BstMWI GCNNNNNNNGC 2 cut(s) 802, 1743
BstNI CCWGG 4 cut(s) 175, 386, 2349, 2375
BstNSI RCATGY 2 cut(s) 1726, 2147
BstSCI CCNGG 7 cut(s) 156, 157, 173, 384, 629, 2347, 2373
BstSFI CTRYAG 2 cut(s) 929, 1416
BstV1I GCAGC 4 cut(s) 73, 80, 1667, 1670
BstV2I GAAGAC 2 cut(s) 318, 357
BstX2I RGATCY 2 cut(s) 260, 2188
BstYI RGATCY 2 cut(s) 260, 2188
BstZ17I GTATAC 2 cut(s) 840, 1379
BsuI GTATCC 2 cut(s) 397, 1417
BsuRI GGCC 5 cut(s) 129, 173, 634, 791, 1547
BtsCI GGATG 5 cut(s) 488, 970, 1263, 1710, 2113
BtsIMutI CAGTG 6 cut(s) 819, 1004, 1454, 1654, 2301, 2445
Cac8I GCNNGC 1 cut(s) 929
CaiI CAGNNNCTG 1 cut(s) 1343
CfoI GCGC 1 cut(s) 2266
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 4 cut(s) 7, 632, 790, 1532
Cfr9I CCCGGG 1 cut(s) 157
CseI GACGC 2 cut(s) 490, 1959
CsiI ACCWGGT 1 cut(s) 384
Csp6I GTAC 7 cut(s) 193, 627, 660, 941, 1290, 1720, 2203
CviAII CATG 6 cut(s) 257, 1723, 1807, 2144, 2148, 2305
CviQI GTAC 7 cut(s) 193, 627, 660, 941, 1290, 1720, 2203
DdeI CTNAG 4 cut(s) 588, 744, 2010, 2228
DpnI GATC 7 cut(s) 262, 303, 610, 1429, 1758, 2121, 2190
DpnII GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 4 cut(s) 155, 1385, 1608, 2139
EarI CTCTTC 4 cut(s) 155, 1385, 1608, 2139
Eco130I CCWWGG 2 cut(s) 114, 635
Eco147I AGGCCT 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 1863
Eco32I GATATC 1 cut(s) 1159
Eco47I GGWCC 2 cut(s) 7, 1532
Eco47III AGCGCT 1 cut(s) 2265
Eco57I CTGAAG 2 cut(s) 475, 1406
Eco88I CYCGRG 3 cut(s) 157, 1557, 1818
EcoO109I RGGNCCY 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 1565
EcoRII CCWGG 4 cut(s) 173, 384, 2347, 2373
EcoRV GATATC 1 cut(s) 1159
EcoT14I CCWWGG 2 cut(s) 114, 635
EcoT22I ATGCAT 1 cut(s) 2149
ErhI CCWWGG 2 cut(s) 114, 635
FaeI CATG 6 cut(s) 260, 1726, 1810, 2147, 2151, 2308
FaqI GGGAC 3 cut(s) 500, 2002, 2154
FatI CATG 6 cut(s) 256, 1722, 1806, 2143, 2147, 2304
FauNDI CATATG 3 cut(s) 428, 1993, 2209
FblI GTMKAC 2 cut(s) 839, 1378
Fnu4HI GCNGC 5 cut(s) 69, 87, 797, 1681, 1684
FokI GGATG 5 cut(s) 495, 977, 1270, 1717, 2100
Fsp4HI GCNGC 5 cut(s) 69, 87, 797, 1681, 1684
FspBI CTAG 6 cut(s) 534, 800, 1149, 1274, 1514, 2448
GlaI GCGC 1 cut(s) 2265
GluI GCNGC 5 cut(s) 69, 87, 797, 1681, 1684
GsaI CCCAGC 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 2267
HaeIII GGCC 5 cut(s) 129, 173, 634, 791, 1547
HapII CCGG 5 cut(s) 63, 126, 158, 630, 1293
HgaI GACGC 2 cut(s) 490, 1959
HhaI GCGC 1 cut(s) 2266
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 6 cut(s) 260, 1726, 1810, 2147, 2151, 2308
Hin6I GCGC 1 cut(s) 2264
HinP1I GCGC 1 cut(s) 2264
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 2 cut(s) 133, 1735
HpaII CCGG 5 cut(s) 63, 126, 158, 630, 1293
HphI GGTGA 2 cut(s) 91, 2114
Hpy166II GTNNAC 4 cut(s) 505, 840, 1379, 1540
Hpy188III TCNNGA 7 cut(s) 63, 514, 665, 1425, 1848, 2009, 2117
Hpy8I GTNNAC 4 cut(s) 505, 840, 1379, 1540
HpyAV CCTTC 7 cut(s) 469, 806, 1012, 1019, 1294, 1392, 2312
HpyCH4III ACNGT 3 cut(s) 1458, 2157, 2440
HpyCH4IV ACGT 1 cut(s) 1047
HpyF10VI GCNNNNNNNGC 2 cut(s) 802, 1743
HpyF3I CTNAG 4 cut(s) 588, 744, 2010, 2228
HpySE526I ACGT 1 cut(s) 1047
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 6 cut(s) 260, 1726, 1810, 2147, 2151, 2308
HspAI GCGC 1 cut(s) 2264
Kpn2I TCCGGA 1 cut(s) 62
KpnI GGTACC 1 cut(s) 1293
Kzo9I GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
LmnI GCTCC 4 cut(s) 65, 881, 2179, 2344
Lsp1109I GCAGC 4 cut(s) 73, 80, 1667, 1670
LweI GCATC 2 cut(s) 1248, 1695
MabI ACCWGGT 1 cut(s) 384
MaeI CTAG 6 cut(s) 534, 800, 1149, 1274, 1514, 2448
MaeII ACGT 1 cut(s) 1047
MaeIII GTNAC 6 cut(s) 536, 860, 1163, 1630, 2151, 2323
MalI GATC 7 cut(s) 262, 303, 610, 1429, 1758, 2121, 2190
MboI GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
MfeI CAATTG 3 cut(s) 56, 1473, 2019
MflI RGATCY 2 cut(s) 260, 2188
MlyI GAGTC 3 cut(s) 141, 1578, 1838
MmeI TCCRAC 4 cut(s) 230, 460, 1047, 1675
Mph1103I ATGCAT 1 cut(s) 2149
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 3 cut(s) 620, 2032, 2368
MseI TTAA 9 cut(s) 17, 972, 1434, 1467, 1620, 1668, 1853, 1875, 2309
MslI CAYNNNNRTG 3 cut(s) 1088, 1256, 1805
MspA1I CMGCKG 2 cut(s) 71, 796
MspCI CTTAAG 1 cut(s) 1874
MspI CCGG 5 cut(s) 63, 126, 158, 630, 1293
MspR9I CCNGG 7 cut(s) 158, 159, 175, 386, 631, 2349, 2375
MunI CAATTG 3 cut(s) 56, 1473, 2019
Mva1269I GAATGC 1 cut(s) 2235
MvaI CCWGG 4 cut(s) 175, 386, 2349, 2375
MwoI GCNNNNNNNGC 2 cut(s) 802, 1743
NciI CCSGG 3 cut(s) 158, 159, 631
NdeI CATATG 3 cut(s) 428, 1993, 2209
NdeII GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
NlaIII CATG 6 cut(s) 260, 1726, 1810, 2147, 2151, 2308
NlaIV GGNNCC 3 cut(s) 262, 1291, 2346
NmuCI GTSAC 2 cut(s) 536, 860
NsiI ATGCAT 1 cut(s) 2149
NspI RCATGY 2 cut(s) 1726, 2147
NspV TTCGAA 2 cut(s) 138, 1042
PaeR7I CTCGAG 1 cut(s) 1818
PceI AGGCCT 1 cut(s) 173
PctI GAATGC 1 cut(s) 2235
PdmI GAANNNNTTC 3 cut(s) 620, 2032, 2368
PfeI GAWTC 8 cut(s) 251, 413, 510, 668, 1277, 1595, 1660, 2352
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 5 cut(s) 70, 88, 798, 1682, 1685
PleI GAGTC 3 cut(s) 141, 1578, 1838
PpsI GAGTC 3 cut(s) 141, 1578, 1838
PpuMI RGGWCCY 1 cut(s) 7
PshBI ATTAAT 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 4 cut(s) 173, 384, 2347, 2373
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 4 cut(s) 173, 384, 2347, 2373
PspN4I GGNNCC 3 cut(s) 262, 1291, 2346
PspPI GGNCC 4 cut(s) 7, 632, 790, 1532
PspPPI RGGWCCY 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 1818
PstI CTGCAG 1 cut(s) 933
PstNI CAGNNNCTG 1 cut(s) 1343
PsuI RGATCY 2 cut(s) 260, 2188
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 194, 628, 661, 942, 1291, 1721, 2204
RsaNI GTAC 7 cut(s) 193, 627, 660, 941, 1290, 1720, 2203
RseI CAYNNNNRTG 3 cut(s) 1088, 1256, 1805
SaqAI TTAA 9 cut(s) 17, 972, 1434, 1467, 1620, 1668, 1853, 1875, 2309
SatI GCNGC 5 cut(s) 69, 87, 797, 1681, 1684
Sau3AI GATC 7 cut(s) 260, 301, 608, 1427, 1756, 2119, 2188
Sau96I GGNCC 4 cut(s) 7, 632, 790, 1532
SbfI CCTGCAGG 1 cut(s) 933
SchI GAGTC 3 cut(s) 141, 1578, 1838
ScrFI CCNGG 7 cut(s) 158, 159, 175, 386, 631, 2349, 2375
SdaI CCTGCAGG 1 cut(s) 933
SexAI ACCWGGT 1 cut(s) 384
SfaNI GCATC 2 cut(s) 1248, 1695
SfcI CTRYAG 2 cut(s) 929, 1416
Sfr274I CTCGAG 1 cut(s) 1818
SfuI TTCGAA 2 cut(s) 138, 1042
SinI GGWCC 2 cut(s) 7, 1532
SlaI CTCGAG 1 cut(s) 1818
SmaI CCCGGG 1 cut(s) 159
SmiMI CAYNNNNRTG 3 cut(s) 1088, 1256, 1805
SmlI CTYRAG 4 cut(s) 1120, 1423, 1818, 1874
SmoI CTYRAG 4 cut(s) 1120, 1423, 1818, 1874
Sse8387I CCTGCAGG 1 cut(s) 933
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 2 cut(s) 796, 1482
SspMI CTAG 6 cut(s) 534, 800, 1149, 1274, 1514, 2448
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 7 cut(s) 156, 157, 173, 384, 629, 2347, 2373
StyI CCWWGG 2 cut(s) 114, 635
TaaI ACNGT 3 cut(s) 1458, 2157, 2440
TaiI ACGT 1 cut(s) 1050
TaqI TCGA 6 cut(s) 138, 1042, 1755, 1819, 2391, 2435
TaqII GACCGA 2 cut(s) 1315, 1520
TatI WGTACW 1 cut(s) 1719
TauI GCSGC 1 cut(s) 799
TfiI GAWTC 8 cut(s) 251, 413, 510, 668, 1277, 1595, 1660, 2352
Tru1I TTAA 9 cut(s) 17, 972, 1434, 1467, 1620, 1668, 1853, 1875, 2309
Tru9I TTAA 9 cut(s) 17, 972, 1434, 1467, 1620, 1668, 1853, 1875, 2309
TscAI CASTG 6 cut(s) 826, 1011, 1461, 1661, 2301, 2445
TseFI GTSAC 2 cut(s) 536, 860
TseI GCWGC 4 cut(s) 68, 86, 1680, 1683
Tsp45I GTSAC 2 cut(s) 536, 860
TspGWI ACGGA 3 cut(s) 614, 1151, 1182
TspMI CCCGGG 1 cut(s) 157
TspRI CASTG 6 cut(s) 826, 1011, 1461, 1661, 2301, 2445
Van91I CCANNNNNTGG 1 cut(s) 548
Vha464I CTTAAG 1 cut(s) 1874
VpaK11BI GGWCC 2 cut(s) 7, 1532
VspI ATTAAT 1 cut(s) 17
XapI RAATTY 3 cut(s) 199, 891, 1565
XceI RCATGY 2 cut(s) 1726, 2147
XcmI CCANNNNNNNNNTGG 2 cut(s) 1735, 2437
XhoI CTCGAG 1 cut(s) 1818
XmaI CCCGGG 1 cut(s) 157
XmiI GTMKAC 2 cut(s) 839, 1378
XmnI GAANNNNTTC 3 cut(s) 620, 2032, 2368
XspI CTAG 6 cut(s) 534, 800, 1149, 1274, 1514, 2448
Zsp2I ATGCAT 1 cut(s) 2149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.