RchiOBHm_Chr5g0036211

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001151
Physical Location & Seq
Reverse (-)
30411259 .. 30414960
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31503

Sequence Viewer

Length: 2301 bp
ATGAAGGGCCTTCCTTTCATCTTATTCATCTCTCTTTTCCATGTTTTAGTGTTCAGTTTATGCAGTGCAGTTGATACTATATCTCCAACACAATCCATCACCAGCTCCAATACCTTGGTTTCTTCCGGTGGAAGATTTGAATTAGGACTCTTCTCCTCCGGAAACTCTAAAGCATGGTATTTAGGAATTTGGTACACCAAGTTTTCGAATATAGTTGTATGGGTCGCCAACAGAGAAAACCCAATTCCAGGTTCAGATGGAGCCCTGACATTAACCGAAAATGGTACTTTGGTCCTTTTAGACCAGATGAACAACACTGTATGGTATTCTAATTTTTCCACAGTAGTTGAAGATCCTGTTGCACAGCTTTTGGAGACTGGAAATTTTGTTGTCAGAGACAAGGCTACAGCGGGTTCGGAAAGTTTTGTATGGCAGAGTTTTGATTTCCCATCGGATACATTGTTAGCAGGGATGAAGCTTGGATGGGATTTCAGAACCGGGCTCAACAAGTTCTTGACTTCTTGGAAAAATGCTAGTGATCCATCTCTTGGTGAGTATACCTATAGGATTGTTAATCTTGCTTTGCCTCAGCTTGTTCTTGCTAAGGGATCGGAGAAAGAGTTTCGAAGTGGGCCCTGGAATGGTCTTCGGTTTACTGGTTATCCTGTTTCGAACAATGAAATTATAACACCTAGTTATGTGTATGATACAAATGAGTTGTATTACACGTATAATGACAATTCTCTTATTACAAGATTGCGGCTAACTGAAGGAGGTGACATGCAGCGGTTAGTGCTGAATAAAGGAAGCACTGAATGGAACGATATGTATAATTTGCTGAATGATCGATGTGATAGGTACGGGGAGTGTGGCGCAAATGGCATTTGCAGAATTAACAACTCACCCATTTGTGACTGCTTGGACGGGTTTATTCCGAAATCCCAGCAAGAGTGGGTGGTGCTTAATACGACAAGTGGGTGCACAAGGAAGATACAATTGGATTGTCAGAAAGGAGAAGGGTTTCGGGAAATTCAAAACGTAAAACTGCCAGACCTGTTGGATTTTTGGGTCAACAAGAGCCGGAACAAGAAGGAATGCAGGGCAGAGTGTTTGAAGAATTGTTCTTGTATTGCTTATTCTTATTCAGATTACAGTCTTGGAGGCACCGGCTGTCTGATGTGGTTTAAAGACCTTATTGATATCCGTGAGTTTGCTGATGAAAGTGAGCAGCAACGTATTTATATTAGGATGCCAGCTTCAGAACTAGGTTCAAGATCGAATAAGGAAGACATGGAGTTGCCACTGTTTGATTTTCATACAATTGCAGCAGGCACCAATAACTTCTCAGGGACAAACATACTTGGGGAGGGAGGTTTTGGTCCAGTTTACAAGGCCAACCTAGCACAAGAACAACTAGTAGCAGTAAAAAGACTCTCAAAAGATTCCGGGCAAGGTATTGATGAGTTTAAGAATGAAGTTACGATGATAGCCAACCTTCAACACAGGAATCTTGTTAAACTTTTGGGCTGCTGCATTGAAGGACAAGAGAGGATGCTAATCTATGAGTATATGCCTAATAAAAGCTTGAACTGCTTTATTTTCGATGAAAATAGAAAGAAATCGCTTGATTGGCAAAAACGAGTCAACATTATAATTGGAACTGCACGTGGACTTCTCTACCTCCACCAAGACTCCAGATTGAGAATCATTCATAGGGACCTCAAGAGTAGCAACATTTTACTCGACAATGAGCTGAACCCCAAAATATCTGACTTCGGCATAGCAAGAATTTTTGGACTTGATCAGACTGAAGCAAAGACAAAACGAGTGATTGGCACATATGGGTATATGTCTCCCGAGTACGCAATTGATGGGCACTTTTCAGTGAAATCCGATGTCTTTAGTTTTGGAGTGCTTTTGCTAGAGATGGTAAGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGATCACCATCACAGTCTTCTGGGACATGCCTGGCTACTATGGAACAAAGAAAAGGTTTTGGAACTATTAGATCCATGCTTGGAGGACTCATATGTTGAGCTTGAGGTACTAAGATGTATACAAGTTGGTCTATTATGCGTGCAAAAGCGGCCCATCGACAGACCAGTAATGTCATCAGTGGTGTTCATGTTAAGCAACGAGGGAGCAATGTTGCCTGAACCGAAGGAGCCTGGTTTCTTCACAGAACGAAGTTCCATGGATGGTGATACCTTCATTAGTGAAGGAAGAAGCAAAACAGGAAACATGATTACCATTACCACGATGGAAGCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

766

Amino Acids

86.55

Weight (kDa)

5.72

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 176 9.5e-35 D-mannose binding lectin
S_locus_glycop PF00954 208 - 314 1.4e-25 S-locus glycoprotein domain
PAN_2 PF08276 335 - 401 2.6e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 452 - 649 4.4e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 452 - 648 1.4e-44 Protein kinase domain
DUF3403 PF11883 722 - 766 7.8e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 686, 1652
AccB1I GGYRCC 2 cut(s) 1163, 1331
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 2 cut(s) 557, 2083
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 4 cut(s) 410, 760, 787, 2113
AclWI GGATC 4 cut(s) 347, 533, 616, 2030
AcsI RAATTY 4 cut(s) 186, 382, 1029, 1788
AcuI CTGAAG 3 cut(s) 789, 1242, 1830
AcvI CACGTG 1 cut(s) 1667
AfaI GTAC 5 cut(s) 194, 286, 860, 1862, 2073
AfiI CCNNNNNNNGG 3 cut(s) 248, 548, 641
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 8 cut(s) 140, 350, 1034, 1114, 1272, 1499, 1538, 1588
AhlI ACTAGT 1 cut(s) 1414
AjnI CCWGG 4 cut(s) 247, 635, 1994, 2194
AjuI GAANNNNNNNTTGG 4 cut(s) 235, 267, 980, 1012
AluBI AGCT 9 cut(s) 105, 367, 478, 592, 1256, 1584, 1753, 2065, 2294
AluI AGCT 9 cut(s) 105, 367, 478, 592, 1256, 1584, 1753, 2065, 2294
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 3 cut(s) 368, 390, 1857
Alw44I GTGCAC 1 cut(s) 979
AlwI GGATC 4 cut(s) 347, 533, 616, 2030
Ama87I CYCGRG 1 cut(s) 1856
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 4 cut(s) 7, 632, 1392, 2114
ApaI GGGCCC 1 cut(s) 636
ApaLI GTGCAC 1 cut(s) 979
ApeKI GCWGC 5 cut(s) 784, 1228, 1325, 1527, 1530
ApoI RAATTY 4 cut(s) 186, 382, 1029, 1788
Asp700I GAANNNNTTC 1 cut(s) 1020
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 7 cut(s) 7, 292, 632, 633, 1379, 1717, 2115
AsuC2I CCSGG 2 cut(s) 499, 1447
AsuHPI GGTGA 6 cut(s) 91, 563, 788, 894, 1961, 2240
AsuII TTCGAA 3 cut(s) 206, 625, 671
AvaI CYCGRG 1 cut(s) 1856
AvaII GGWCC 3 cut(s) 292, 1379, 1717
BaeGI GKGCMC 3 cut(s) 636, 983, 1878
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BanI GGYRCC 2 cut(s) 1163, 1331
BanII GRGCYC 3 cut(s) 265, 504, 636
BbrPI CACGTG 1 cut(s) 1667
BbsI GAAGAC 3 cut(s) 638, 1293, 1973
Bbv12I GWGCWC 1 cut(s) 983
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 5 cut(s) 796, 1240, 1337, 1514, 1517
BciT130I CCWGG 4 cut(s) 249, 637, 1996, 2196
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 1 cut(s) 1801
BcnI CCSGG 2 cut(s) 499, 1447
BcoDI GTCTC 3 cut(s) 368, 390, 1857
BcuI ACTAGT 1 cut(s) 1414
BfaI CTAG 6 cut(s) 534, 693, 1265, 1400, 1415, 1922
BfmI CTRYAG 2 cut(s) 405, 562
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 7 cut(s) 761, 785, 1229, 1326, 1528, 1531, 2114
BlsI GCNGC 7 cut(s) 762, 786, 1230, 1327, 1529, 1532, 2115
Bme1390I CCNGG 6 cut(s) 249, 499, 637, 1447, 1996, 2196
Bme18I GGWCC 3 cut(s) 292, 1379, 1717
BmeT110I CYCGRG 1 cut(s) 1856
BmgT120I GGNCC 7 cut(s) 7, 292, 632, 633, 1379, 1717, 2115
BmiI GGNNCC 6 cut(s) 262, 634, 1165, 1333, 1718, 2193
BmrFI CCNGG 6 cut(s) 249, 499, 637, 1447, 1996, 2196
BmsI GCATC 2 cut(s) 1239, 1542
BpiI GAAGAC 3 cut(s) 638, 1293, 1973
BpmI CTGGAG 1 cut(s) 1678
Bpu10I CCTNAGC 2 cut(s) 588, 603
Bpu14I TTCGAA 3 cut(s) 206, 625, 671
BpuEI CTTGAG 2 cut(s) 1706, 2087
BpuMI CCSGG 2 cut(s) 499, 1447
Bsa29I ATCGAT 1 cut(s) 847
BsaAI YACGTR 2 cut(s) 729, 1667
BsaBI GATNNNNATC 2 cut(s) 1556, 1971
BsaJI CCNNGG 3 cut(s) 114, 635, 2220
BsaWI WCCGGW 2 cut(s) 125, 158
BsaXI ACNNNNNCTCC 6 cut(s) 67, 97, 605, 635, 1676, 1706
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 548, 641
Bse118I RCCGGY 1 cut(s) 1166
Bse1I ACTGG 4 cut(s) 382, 661, 1382, 2129
Bse3DI GCAATG 1 cut(s) 2178
Bse8I GATNNNNATC 2 cut(s) 1556, 1971
BseAI TCCGGA 1 cut(s) 158
BseBI CCWGG 4 cut(s) 249, 637, 1996, 2196
BseCI ATCGAT 1 cut(s) 847
BseDI CCNNGG 3 cut(s) 114, 635, 2220
BseGI GGATG 6 cut(s) 477, 488, 1254, 1557, 1960, 2230
BseJI GATNNNNATC 2 cut(s) 1556, 1971
BseLI CCNNNNNNNGG 3 cut(s) 248, 548, 641
BseMI GCAATG 1 cut(s) 2178
BseMII CTCAG 2 cut(s) 602, 1359
BseNI ACTGG 4 cut(s) 382, 661, 1382, 2129
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 3 cut(s) 636, 983, 1878
BseXI GCAGC 5 cut(s) 796, 1240, 1337, 1514, 1517
BseYI CCCAGC 1 cut(s) 942
BsgI GTGCAG 2 cut(s) 87, 1647
BshFI GGCC 4 cut(s) 9, 634, 1394, 2116
BshNI GGYRCC 2 cut(s) 1163, 1331
BshVI ATCGAT 1 cut(s) 847
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 1856
BsiSI CCGG 6 cut(s) 126, 159, 498, 1081, 1167, 1446
BslFI GGGAC 3 cut(s) 1363, 1730, 2001
BslI CCNNNNNNNGG 3 cut(s) 248, 548, 641
BsmAI GTCTC 3 cut(s) 368, 390, 1857
BsmFI GGGAC 3 cut(s) 1363, 1730, 2001
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 4 cut(s) 9, 634, 1394, 2116
BsoBI CYCGRG 1 cut(s) 1856
Bsp119I TTCGAA 3 cut(s) 206, 625, 671
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 5 cut(s) 265, 504, 636, 983, 1878
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
Bsp19I CCATGG 1 cut(s) 2220
BspACI CCGC 4 cut(s) 410, 760, 787, 2113
BspANI GGCC 4 cut(s) 9, 634, 1394, 2116
BspCNI CTCAG 2 cut(s) 601, 1358
BspDI ATCGAT 1 cut(s) 847
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 6 cut(s) 262, 634, 1165, 1333, 1718, 2193
BspPI GGATC 4 cut(s) 347, 533, 616, 2030
BspT104I TTCGAA 3 cut(s) 206, 625, 671
BspT107I GGYRCC 2 cut(s) 1163, 1331
BsrDI GCAATG 1 cut(s) 2178
BsrFI RCCGGY 1 cut(s) 1166
BsrI ACTGG 4 cut(s) 382, 661, 1382, 2129
BssAI RCCGGY 1 cut(s) 1166
BssECI CCNNGG 3 cut(s) 114, 635, 2220
BssMI GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
BssNAI GTATAC 2 cut(s) 558, 2084
BssT1I CCWWGG 2 cut(s) 114, 2220
Bst1107I GTATAC 2 cut(s) 558, 2084
Bst2UI CCWGG 4 cut(s) 249, 637, 1996, 2196
Bst4CI ACNGT 5 cut(s) 319, 343, 1154, 1305, 1979
Bst6I CTCTTC 1 cut(s) 155
BstBAI YACGTR 2 cut(s) 729, 1667
BstBI TTCGAA 3 cut(s) 206, 625, 671
BstC8I GCNNGC 3 cut(s) 1254, 1330, 2105
BstDEI CTNAG 4 cut(s) 588, 603, 1345, 2075
BstDSI CCRYGG 1 cut(s) 2220
BstF5I GGATG 6 cut(s) 477, 488, 1254, 1557, 1960, 2230
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 8 cut(s) 355, 541, 611, 847, 1277, 1804, 1969, 2038
BstMAI GTCTC 3 cut(s) 368, 390, 1857
BstMBI GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
BstMWI GCNNNNNNNGC 6 cut(s) 793, 879, 1400, 1590, 1630, 2113
BstNI CCWGG 4 cut(s) 249, 637, 1996, 2196
BstNSI RCATGY 2 cut(s) 784, 1994
BstSCI CCNGG 6 cut(s) 247, 497, 635, 1445, 1994, 2194
BstSFI CTRYAG 2 cut(s) 405, 562
BstSLI GKGCMC 3 cut(s) 636, 983, 1878
BstV1I GCAGC 5 cut(s) 796, 1240, 1337, 1514, 1517
BstV2I GAAGAC 3 cut(s) 638, 1293, 1973
BstX2I RGATCY 2 cut(s) 352, 2035
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 352, 2035
BstZ17I GTATAC 2 cut(s) 558, 2084
Bsu15I ATCGAT 1 cut(s) 847
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 9, 634, 1394, 2116
BsuTUI ATCGAT 1 cut(s) 847
BtgI CCRYGG 1 cut(s) 2220
BtsCI GGATG 6 cut(s) 477, 488, 1254, 1557, 1960, 2230
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 6 cut(s) 70, 315, 810, 1301, 1890, 2148
Cac8I GCNNGC 3 cut(s) 1254, 1330, 2105
CfoI GCGC 1 cut(s) 875
Cfr10I RCCGGY 1 cut(s) 1166
Cfr13I GGNCC 7 cut(s) 7, 292, 632, 633, 1379, 1717, 2115
ClaI ATCGAT 1 cut(s) 847
Csp6I GTAC 5 cut(s) 193, 285, 859, 1861, 2072
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 9 cut(s) 41, 174, 781, 1291, 1991, 2040, 2152, 2221, 2269
CviQI GTAC 5 cut(s) 193, 285, 859, 1861, 2072
DdeI CTNAG 4 cut(s) 588, 603, 1345, 2075
DpnI GATC 8 cut(s) 354, 540, 610, 846, 1276, 1803, 1968, 2037
DpnII GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
DraI TTTAAA 1 cut(s) 1186
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Eco130I CCWWGG 2 cut(s) 114, 2220
Eco24I GRGCYC 3 cut(s) 265, 504, 636
Eco32I GATATC 1 cut(s) 1201
Eco47I GGWCC 3 cut(s) 292, 1379, 1717
Eco57I CTGAAG 3 cut(s) 789, 1242, 1830
Eco72I CACGTG 1 cut(s) 1667
Eco88I CYCGRG 1 cut(s) 1856
EcoO109I RGGNCCY 3 cut(s) 7, 633, 1717
EcoRII CCWGG 4 cut(s) 247, 635, 1994, 2194
EcoRV GATATC 1 cut(s) 1201
EcoT14I CCWWGG 2 cut(s) 114, 2220
EcoT38I GRGCYC 3 cut(s) 265, 504, 636
ErhI CCWWGG 2 cut(s) 114, 2220
FaeI CATG 9 cut(s) 44, 177, 784, 1294, 1994, 2043, 2155, 2224, 2272
FaqI GGGAC 3 cut(s) 1363, 1730, 2001
FatI CATG 9 cut(s) 40, 173, 780, 1290, 1990, 2039, 2151, 2220, 2268
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 2 cut(s) 1840, 2056
FbaI TGATCA 1 cut(s) 1801
FblI GTMKAC 2 cut(s) 557, 2083
Fnu4HI GCNGC 7 cut(s) 761, 785, 1229, 1326, 1528, 1531, 2114
FokI GGATG 6 cut(s) 484, 495, 1261, 1564, 1947, 2237
FriOI GRGCYC 3 cut(s) 265, 504, 636
Fsp4HI GCNGC 7 cut(s) 761, 785, 1229, 1326, 1528, 1531, 2114
FspBI CTAG 6 cut(s) 534, 693, 1265, 1400, 1415, 1922
GlaI GCGC 1 cut(s) 874
GluI GCNGC 7 cut(s) 761, 785, 1229, 1326, 1528, 1531, 2114
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 1678
HaeIII GGCC 4 cut(s) 9, 634, 1394, 2116
HapII CCGG 6 cut(s) 126, 159, 498, 1081, 1167, 1446
HhaI GCGC 1 cut(s) 875
Hin1II CATG 9 cut(s) 44, 177, 784, 1294, 1994, 2043, 2155, 2224, 2272
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HincII GTYRAC 2 cut(s) 1072, 1645
HindII GTYRAC 2 cut(s) 1072, 1645
HindIII AAGCTT 2 cut(s) 476, 1582
HinfI GANTC 8 cut(s) 147, 1431, 1442, 1507, 1641, 1691, 1704, 2051
HpaII CCGG 6 cut(s) 126, 159, 498, 1081, 1167, 1446
HphI GGTGA 6 cut(s) 91, 563, 788, 894, 1961, 2240
Hpy166II GTNNAC 9 cut(s) 195, 558, 654, 981, 1072, 1387, 1645, 1670, 2084
Hpy188III TCNNGA 8 cut(s) 159, 514, 1025, 1272, 1695, 1723, 1856, 1964
Hpy8I GTNNAC 9 cut(s) 195, 558, 654, 981, 1072, 1387, 1645, 1670, 2084
HpyAV CCTTC 9 cut(s) 20, 764, 1010, 1084, 1505, 1532, 2182, 2240, 2245
HpyCH4III ACNGT 5 cut(s) 319, 343, 1154, 1305, 1979
HpyCH4IV ACGT 4 cut(s) 728, 1038, 1234, 1666
HpyF10VI GCNNNNNNNGC 6 cut(s) 793, 879, 1400, 1590, 1630, 2113
HpyF3I CTNAG 4 cut(s) 588, 603, 1345, 2075
HpySE526I ACGT 4 cut(s) 728, 1038, 1234, 1666
Hsp92II CATG 9 cut(s) 44, 177, 784, 1294, 1994, 2043, 2155, 2224, 2272
HspAI GCGC 1 cut(s) 873
Kpn2I TCCGGA 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 1801
Kzo9I GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
LmnI GCTCC 4 cut(s) 110, 260, 2168, 2191
Lsp1109I GCAGC 5 cut(s) 796, 1240, 1337, 1514, 1517
LweI GCATC 2 cut(s) 1239, 1542
MaeI CTAG 6 cut(s) 534, 693, 1265, 1400, 1415, 1922
MaeII ACGT 4 cut(s) 728, 1038, 1234, 1666
MaeIII GTNAC 3 cut(s) 776, 911, 1477
MalI GATC 8 cut(s) 354, 540, 610, 846, 1276, 1803, 1968, 2037
MboI GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
MfeI CAATTG 3 cut(s) 995, 1320, 1866
MflI RGATCY 2 cut(s) 352, 2035
MhlI GDGCHC 5 cut(s) 265, 504, 636, 983, 1878
MlyI GAGTC 5 cut(s) 141, 1425, 1650, 1685, 2045
MmeI TCCRAC 2 cut(s) 110, 1038
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 1020
MseI TTAA 8 cut(s) 272, 573, 894, 963, 1185, 1467, 1515, 2156
MspA1I CMGCKG 2 cut(s) 410, 787
MspI CCGG 6 cut(s) 126, 159, 498, 1081, 1167, 1446
MspR9I CCNGG 6 cut(s) 249, 499, 637, 1447, 1996, 2196
MunI CAATTG 3 cut(s) 995, 1320, 1866
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 4 cut(s) 249, 637, 1996, 2196
MwoI GCNNNNNNNGC 6 cut(s) 793, 879, 1400, 1590, 1630, 2113
NciI CCSGG 2 cut(s) 499, 1447
NcoI CCATGG 1 cut(s) 2220
NdeI CATATG 2 cut(s) 1840, 2056
NdeII GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
NlaIII CATG 9 cut(s) 44, 177, 784, 1294, 1994, 2043, 2155, 2224, 2272
NlaIV GGNNCC 6 cut(s) 262, 634, 1165, 1333, 1718, 2193
NmuCI GTSAC 2 cut(s) 776, 911
NspI RCATGY 2 cut(s) 784, 1994
NspV TTCGAA 3 cut(s) 206, 625, 671
PctI GAATGC 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 3 cut(s) 1442, 1507, 1704
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 7 cut(s) 762, 786, 1230, 1327, 1529, 1532, 2115
PleI GAGTC 5 cut(s) 141, 1425, 1649, 1685, 2045
PmaCI CACGTG 1 cut(s) 1667
PmlI CACGTG 1 cut(s) 1667
PpsI GAGTC 5 cut(s) 141, 1425, 1649, 1685, 2045
Ppu21I YACGTR 2 cut(s) 729, 1667
PpuMI RGGWCCY 1 cut(s) 1717
PsiI TTATAA 2 cut(s) 686, 1652
Psp5II RGGWCCY 1 cut(s) 1717
Psp6I CCWGG 4 cut(s) 247, 635, 1994, 2194
PspCI CACGTG 1 cut(s) 1667
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 4 cut(s) 247, 635, 1994, 2194
PspN4I GGNNCC 6 cut(s) 262, 634, 1165, 1333, 1718, 2193
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 7 cut(s) 7, 292, 632, 633, 1379, 1717, 2115
PspPPI RGGWCCY 1 cut(s) 1717
PsuI RGATCY 2 cut(s) 352, 2035
RsaI GTAC 5 cut(s) 194, 286, 860, 1862, 2073
RsaNI GTAC 5 cut(s) 193, 285, 859, 1861, 2072
SaqAI TTAA 8 cut(s) 272, 573, 894, 963, 1185, 1467, 1515, 2156
SatI GCNGC 7 cut(s) 761, 785, 1229, 1326, 1528, 1531, 2114
Sau3AI GATC 8 cut(s) 352, 538, 608, 844, 1274, 1801, 1966, 2035
Sau96I GGNCC 7 cut(s) 7, 292, 632, 633, 1379, 1717, 2115
SchI GAGTC 5 cut(s) 141, 1425, 1650, 1685, 2045
ScrFI CCNGG 6 cut(s) 249, 499, 637, 1447, 1996, 2196
SduI GDGCHC 5 cut(s) 265, 504, 636, 983, 1878
SfaNI GCATC 2 cut(s) 1239, 1542
SfcI CTRYAG 2 cut(s) 405, 562
SfuI TTCGAA 3 cut(s) 206, 625, 671
SinI GGWCC 3 cut(s) 292, 1379, 1717
SmlI CTYRAG 2 cut(s) 1721, 2066
SmoI CTYRAG 2 cut(s) 1721, 2066
SpeI ACTAGT 1 cut(s) 1414
SsiI CCGC 4 cut(s) 410, 760, 787, 2113
SspMI CTAG 6 cut(s) 534, 693, 1265, 1400, 1415, 1922
StyD4I CCNGG 6 cut(s) 247, 497, 635, 1445, 1994, 2194
StyI CCWWGG 2 cut(s) 114, 2220
TaaI ACNGT 5 cut(s) 319, 343, 1154, 1305, 1979
TaiI ACGT 4 cut(s) 731, 1041, 1237, 1669
TaqI TCGA 9 cut(s) 206, 625, 671, 847, 1277, 1602, 1743, 2121, 2296
TauI GCSGC 2 cut(s) 763, 2116
TfiI GAWTC 3 cut(s) 1442, 1507, 1704
Tru1I TTAA 8 cut(s) 272, 573, 894, 963, 1185, 1467, 1515, 2156
Tru9I TTAA 8 cut(s) 272, 573, 894, 963, 1185, 1467, 1515, 2156
TscAI CASTG 6 cut(s) 70, 322, 817, 1308, 1890, 2148
TseFI GTSAC 2 cut(s) 776, 911
TseI GCWGC 5 cut(s) 784, 1228, 1325, 1527, 1530
Tsp45I GTSAC 2 cut(s) 776, 911
TspGWI ACGGA 1 cut(s) 1193
TspRI CASTG 6 cut(s) 70, 322, 817, 1308, 1890, 2148
Van91I CCANNNNNTGG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 979
VpaK11BI GGWCC 3 cut(s) 292, 1379, 1717
XapI RAATTY 4 cut(s) 186, 382, 1029, 1788
XceI RCATGY 2 cut(s) 784, 1994
XcmI CCANNNNNNNNNTGG 1 cut(s) 2284
XmiI GTMKAC 2 cut(s) 557, 2083
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 6 cut(s) 534, 693, 1265, 1400, 1415, 1922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.