FvH4_3g21270

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001151
Physical Location & Seq
Reverse (-)
14239180 .. 14242982
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21270.t3

Sequence Viewer

Length: 2166 bp
ATGACGTTGTCCAAAAATGGAAGTTTAGTCCTTTTAGACCAAATGAATAGCACTATTTGGTCTTCTAGTCCTTCCAGAGAAGCTGAAGATCCTGTTGCACAGCTTTTGGACACTGGAAACCTTGTTGTTATAGACAAGGCTCTGACTAGTTCCGAAAGTTATATATGGCAAAGTTTTGATTTCCCATCGGACACACTGCTACCAGGAATGAGGCTGCTATTGAACTTCAAAACAGGTCCAAACCAATTCTTGACTTCTTGGGAAAATGCTAGTGACCCGTCTCTTGGACTATATACATATAAGATTGAAAACATTGTGTTGCCTCAGCTTGTTCTTGCTCAAGGATCAAAGAAACAATTCCGCAGTGGACCTTGGAATGGTCTTCGGTTTACTGGTCTTCCTGATTCAAGCAACGAAATTTTGCAACCAAGTTATGTTTATAACACAAATGAGTTGTATTATATCTATAAAGCTAATGACAATTCTGTTATTACCCGATCAAAGCTAACTGAAACAGGTGAGGTGCAAAAGCTAGTGCTGAATAAAGGGAGTACTGAATGGGCTGTTATGTATACATTGCAGAATGATAGATGCGACAATTACGGAGAGTGTGGTGCAAATGGCATATGCAAAGTTGACAGAACACCCATCTGTGAGTGCTTGCAGGGGTTTGTCCCAAAATCACACCAAGAATGGGAGGTTCTCAATTGGTCAAGTGGTTGCAAGAGAGAGACACCATTGGATTGCCAGAAGGAGGAAGGGTTTTTGAAATTTCAAAATATAAAACTTCCAGACCTGTTGGATTTTTCGGTGAATAACAGCATGAACATTAAGGAATGTGAAGCAGAGTGTTTGAAGGATTGTTCTTGTGTAGCTTATGCTAAATCAAATATGAGTACTGGAGGCATTGGCTGTCTGATGTGGTTCGGAGAGCTTATAGATATGCGTGAGTTCATTGATGAAGTTAATGATCAAGATCTATATATTCGGATGCCAGCTTCAGAACTAGGCAATACTAGTCAGAAGGACAAAAGAGTAGTATTGATCTTGGTCATATCAGCAGCTGCTGTACTTCTTTTTCTGGGTCTCTCATGCTGGTGTATTGTTCTGAAGAAGAGAGCAAAGCTAAAGGGTTCAAGATCAAGCAAGGAAGACATAGAGTTGCCACTATTTGATTTTCATACAATTGAAATTGGCACCAATTACTTTTCCTGGCAAAACAAACTTGGAGAGGGAGGTTTTGGTCCAGTTTACAAGGCTAACCTAAGACAAGATGAATTAGTAGCAGTAAAGAGGCTCTCAAGAGGTTCTGGACAAGGTCTTAAAGAGTTTAGAAATGAAGTTACAATGATAGCCAATCTTCAACACCGGAATCTTGTTAAACTTTTAGGCTGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCCAACAAAAGCTTGGACTTCTTTATCTTTGATCAAAACAGAAAGAAGCTGCTTAATTGGCAAAAGCGGTTTGACATTATCATGGGAATTGCACGAGGACTTCTCTACCTCCACCAAGACTCGAGATTAAGAATCATTCATAGGGACCTTAAGAGTAGCAACATCTTGCTAGACGATGAGCTGGCCCCAAAGATATCTGACTTTGGCATAGCAAGAATTTTTGAACAAAATCAAACTGAAGGAAAAACAAAACGAGTAATTGGCACATATGGATATATGTCTCCTGAGTACACCATTGATGGCAAGTTTTCAGTGAAATCGGATGTCTTCAGTTTTGGGGTGCTATTATTAGAGATAATTAGTGGCAGAAAGAACATAGGGTTCAATCATCCAGATCACAACCACAATCTTCTGGGACATGCATGGCTACTATGGAACAAAAACAAGGTTTTAGAACTGTTAGATCCATGCTTGGAGTACTCATACATGGAGTACGAGGTGCTAAGAAGTATTCAAGTGGGTCTATTATGCGTACAAAAGCTACCCAATGACAGGCCAGAAATGTCATCAGCGGTGTTCATGTTATGCAATGAAGAAGCAACATTGCCTGAACCAAAGGAGCCTGGTTTCTTCACAGAAAGAAGCTCCGTAGACAGCGAAACCTTAACAGGAGGAGGGAGAAGCCAAACAGGACTCACAATTACTATTTCGACATTGGAGGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

722

Amino Acids

81.68

Weight (kDa)

5.59

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 175 1.5e-35 D-mannose binding lectin
S_locus_glycop PF00954 208 - 316 7.3e-28 S-locus glycoprotein domain
PAN_2 PF08276 337 - 403 1.4e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 496 - 760 1.2e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 497 - 697 1.8e-44 Protein kinase domain
DUF3403 PF11883 767 - 812 1.1e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 1196
AccI GTMKAC 2 cut(s) 572, 2088
AciI CCGC 3 cut(s) 361, 1504, 2009
AclWI GGATC 3 cut(s) 83, 352, 1895
AcsI RAATTY 3 cut(s) 417, 770, 1653
AcuI CTGAAG 5 cut(s) 105, 984, 1130, 1695, 1750
AfaI GTAC 8 cut(s) 553, 898, 1071, 1433, 1727, 1916, 1931, 1971
AfiI CCNNNNNNNGG 5 cut(s) 284, 377, 694, 754, 1989
AflII CTTAAG 1 cut(s) 1586
AhlI ACTAGT 2 cut(s) 146, 1016
AjnI CCWGG 3 cut(s) 202, 1211, 2059
Alw26I GTCTC 4 cut(s) 285, 725, 1091, 1722
AlwI GGATC 3 cut(s) 83, 352, 1895
AlwNI CAGNNNCTG 2 cut(s) 1064, 1067
Ama87I CYCGRG 1 cut(s) 1558
AoxI GGCC 2 cut(s) 1620, 1991
ApeKI GCWGC 6 cut(s) 214, 1061, 1064, 1392, 1395, 1486
ApoI RAATTY 3 cut(s) 417, 770, 1653
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 5 cut(s) 236, 368, 1244, 1582, 1621
AsuHPI GGTGA 2 cut(s) 530, 823
AvaI CYCGRG 1 cut(s) 1558
AvaII GGWCC 4 cut(s) 236, 368, 1244, 1582
BanI GGYRCC 1 cut(s) 1196
BauI CACGAG 1 cut(s) 1530
BbsI GAAGAC 5 cut(s) 54, 374, 389, 1158, 1756
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 6 cut(s) 201, 1051, 1073, 1379, 1382, 1473
BccI CCATC 3 cut(s) 193, 656, 1730
BciT130I CCWGG 3 cut(s) 204, 1213, 2061
BclI TGATCA 2 cut(s) 970, 1468
BcoDI GTCTC 4 cut(s) 285, 725, 1091, 1722
BcuI ACTAGT 2 cut(s) 146, 1016
BfaI CTAG 8 cut(s) 66, 147, 270, 533, 1007, 1017, 1607, 2160
BfrI CTTAAG 1 cut(s) 1586
BglII AGATCT 1 cut(s) 976
BisI GCNGC 6 cut(s) 215, 1062, 1065, 1393, 1396, 1487
BlsI GCNGC 6 cut(s) 216, 1063, 1066, 1394, 1397, 1488
BmcAI AGTACT 3 cut(s) 553, 898, 1916
Bme1390I CCNGG 3 cut(s) 204, 1213, 2061
Bme18I GGWCC 4 cut(s) 236, 368, 1244, 1582
BmeT110I CYCGRG 1 cut(s) 1558
BmgT120I GGNCC 5 cut(s) 236, 368, 1244, 1582, 1621
BmiI GGNNCC 4 cut(s) 1198, 1583, 1623, 2058
BmrFI CCNGG 3 cut(s) 204, 1213, 2061
BmsI GCATC 3 cut(s) 581, 981, 1407
BpiI GAAGAC 5 cut(s) 54, 374, 389, 1158, 1756
BplI GAGNNNNNCTC 2 cut(s) 1524, 1556
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 324, 1285
BsaBI GATNNNNATC 2 cut(s) 975, 1421
BsaI GGTCTC 1 cut(s) 1091
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 1368
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 377, 694, 754, 1989
Bse1I ACTGG 4 cut(s) 118, 397, 904, 1247
Bse3DI GCAATG 3 cut(s) 575, 2032, 2039
Bse8I GATNNNNATC 2 cut(s) 975, 1421
BseBI CCWGG 3 cut(s) 204, 1213, 2061
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 4 cut(s) 996, 1422, 1765, 1825
BseJI GATNNNNATC 2 cut(s) 975, 1421
BseLI CCNNNNNNNGG 5 cut(s) 284, 377, 694, 754, 1989
BseMI GCAATG 3 cut(s) 575, 2032, 2039
BseMII CTCAG 2 cut(s) 338, 1713
BseNI ACTGG 4 cut(s) 118, 397, 904, 1247
BseRI GAGGAG 1 cut(s) 2124
BseXI GCAGC 6 cut(s) 201, 1051, 1073, 1379, 1382, 1473
BshFI GGCC 2 cut(s) 1622, 1993
BshNI GGYRCC 1 cut(s) 1196
BsiHKCI CYCGRG 1 cut(s) 1558
BsiSI CCGG 1 cut(s) 1369
BslFI GGGAC 3 cut(s) 659, 1595, 1866
BslI CCNNNNNNNGG 5 cut(s) 284, 377, 694, 754, 1989
BsmAI GTCTC 4 cut(s) 285, 725, 1091, 1722
BsmBI CGTCTC 1 cut(s) 285
BsmFI GGGAC 3 cut(s) 659, 1595, 1866
BsnI GGCC 2 cut(s) 1622, 1993
Bso31I GGTCTC 1 cut(s) 1091
BsoBI CYCGRG 1 cut(s) 1558
BspACI CCGC 3 cut(s) 361, 1504, 2009
BspANI GGCC 2 cut(s) 1622, 1993
BspCNI CTCAG 2 cut(s) 337, 1714
BspLI GGNNCC 4 cut(s) 1198, 1583, 1623, 2058
BspPI GGATC 3 cut(s) 83, 352, 1895
BspT107I GGYRCC 1 cut(s) 1196
BspTI CTTAAG 1 cut(s) 1586
BspTNI GGTCTC 1 cut(s) 1091
BsrDI GCAATG 3 cut(s) 575, 2032, 2039
BsrI ACTGG 4 cut(s) 118, 397, 904, 1247
BssECI CCNNGG 1 cut(s) 371
BssNAI GTATAC 1 cut(s) 573
BssSI CACGAG 1 cut(s) 1530
BssT1I CCWWGG 1 cut(s) 371
Bst1107I GTATAC 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 1530
Bst2UI CCWGG 3 cut(s) 204, 1213, 2061
Bst4CI ACNGT 1 cut(s) 1896
Bst6I CTCTTC 1 cut(s) 1109
BstAFI CTTAAG 1 cut(s) 1586
BstC8I GCNNGC 3 cut(s) 662, 996, 1620
BstDEI CTNAG 4 cut(s) 324, 1265, 1722, 1940
BstF5I GGATG 4 cut(s) 996, 1422, 1765, 1825
BstMAI GTCTC 4 cut(s) 285, 725, 1091, 1722
BstMWI GCNNNNNNNGC 1 cut(s) 1495
BstNI CCWGG 3 cut(s) 204, 1213, 2061
BstNSI RCATGY 2 cut(s) 1438, 1859
BstSCI CCNGG 3 cut(s) 202, 1211, 2059
BstV1I GCAGC 6 cut(s) 201, 1051, 1073, 1379, 1382, 1473
BstV2I GAAGAC 5 cut(s) 54, 374, 389, 1158, 1756
BstX2I RGATCY 3 cut(s) 88, 976, 1900
BstYI RGATCY 3 cut(s) 88, 976, 1900
BstZ17I GTATAC 1 cut(s) 573
BsuRI GGCC 2 cut(s) 1622, 1993
BtsCI GGATG 4 cut(s) 996, 1422, 1765, 1825
BtsI GCAGTG 2 cut(s) 194, 370
BtsIMutI CAGTG 4 cut(s) 111, 194, 370, 1755
Cac8I GCNNGC 3 cut(s) 662, 996, 1620
CaiI CAGNNNCTG 2 cut(s) 1064, 1067
Cfr13I GGNCC 5 cut(s) 236, 368, 1244, 1582, 1621
Csp6I GTAC 8 cut(s) 552, 897, 1070, 1432, 1726, 1915, 1930, 1970
CviAII CATG 9 cut(s) 823, 1092, 1435, 1519, 1856, 1860, 1905, 1924, 2017
CviQI GTAC 8 cut(s) 552, 897, 1070, 1432, 1726, 1915, 1930, 1970
DdeI CTNAG 4 cut(s) 324, 1265, 1722, 1940
Eam1104I CTCTTC 1 cut(s) 1109
EarI CTCTTC 1 cut(s) 1109
Eco130I CCWWGG 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 1091
Eco32I GATATC 1 cut(s) 1632
Eco47I GGWCC 4 cut(s) 236, 368, 1244, 1582
Eco57I CTGAAG 5 cut(s) 105, 984, 1130, 1695, 1750
Eco88I CYCGRG 1 cut(s) 1558
EcoO109I RGGNCCY 1 cut(s) 1582
EcoRII CCWGG 3 cut(s) 202, 1211, 2059
EcoRV GATATC 1 cut(s) 1632
EcoT14I CCWWGG 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 1861
ErhI CCWWGG 1 cut(s) 371
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 9 cut(s) 826, 1095, 1438, 1522, 1859, 1863, 1908, 1927, 2020
FaqI GGGAC 3 cut(s) 659, 1595, 1866
FatI CATG 9 cut(s) 822, 1091, 1434, 1518, 1855, 1859, 1904, 1923, 2016
FauNDI CATATG 2 cut(s) 626, 1705
FbaI TGATCA 2 cut(s) 970, 1468
FblI GTMKAC 2 cut(s) 572, 2088
Fnu4HI GCNGC 6 cut(s) 215, 1062, 1065, 1393, 1396, 1487
FokI GGATG 4 cut(s) 1003, 1429, 1772, 1812
Fsp4HI GCNGC 6 cut(s) 215, 1062, 1065, 1393, 1396, 1487
FspBI CTAG 8 cut(s) 66, 147, 270, 533, 1007, 1017, 1607, 2160
GluI GCNGC 6 cut(s) 215, 1062, 1065, 1393, 1396, 1487
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 2 cut(s) 1622, 1993
HapII CCGG 1 cut(s) 1369
Hin1II CATG 9 cut(s) 826, 1095, 1438, 1522, 1859, 1863, 1908, 1927, 2020
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 1447
HinfI GANTC 5 cut(s) 404, 1372, 1556, 1569, 2130
HpaII CCGG 1 cut(s) 1369
HphI GGTGA 2 cut(s) 530, 823
Hpy166II GTNNAC 7 cut(s) 368, 390, 573, 637, 1252, 1728, 2089
Hpy8I GTNNAC 7 cut(s) 368, 390, 573, 637, 1252, 1728, 2089
HpyAV CCTTC 7 cut(s) 81, 745, 752, 850, 1018, 1397, 1670
HpyCH4III ACNGT 1 cut(s) 1896
HpyCH4IV ACGT 1 cut(s) 5
HpyF10VI GCNNNNNNNGC 1 cut(s) 1495
HpyF3I CTNAG 4 cut(s) 324, 1265, 1722, 1940
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 9 cut(s) 826, 1095, 1438, 1522, 1859, 1863, 1908, 1927, 2020
Ksp22I TGATCA 2 cut(s) 970, 1468
LmnI GCTCC 2 cut(s) 2056, 2087
Lsp1109I GCAGC 6 cut(s) 201, 1051, 1073, 1379, 1382, 1473
LweI GCATC 3 cut(s) 581, 981, 1407
MaeI CTAG 8 cut(s) 66, 147, 270, 533, 1007, 1017, 1607, 2160
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 272, 1342
MfeI CAATTG 2 cut(s) 706, 1185
MflI RGATCY 3 cut(s) 88, 976, 1900
MlyI GAGTC 2 cut(s) 1550, 2124
MmeI TCCRAC 1 cut(s) 780
Mph1103I ATGCAT 1 cut(s) 1861
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 8 cut(s) 831, 966, 1323, 1380, 1491, 1565, 1587, 2102
MslI CAYNNNNRTG 2 cut(s) 1096, 1517
MspA1I CMGCKG 2 cut(s) 1064, 2009
MspCI CTTAAG 1 cut(s) 1586
MspI CCGG 1 cut(s) 1369
MspR9I CCNGG 3 cut(s) 204, 1213, 2061
MunI CAATTG 2 cut(s) 706, 1185
MvaI CCWGG 3 cut(s) 204, 1213, 2061
MwoI GCNNNNNNNGC 1 cut(s) 1495
NdeI CATATG 2 cut(s) 626, 1705
NlaIII CATG 9 cut(s) 826, 1095, 1438, 1522, 1859, 1863, 1908, 1927, 2020
NlaIV GGNNCC 4 cut(s) 1198, 1583, 1623, 2058
NmuCI GTSAC 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 1861
NspI RCATGY 2 cut(s) 1438, 1859
PaeR7I CTCGAG 1 cut(s) 1558
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 3 cut(s) 404, 1372, 1569
PflFI GACNNNGTC 2 cut(s) 7, 1317
PkrI GCNGC 6 cut(s) 216, 1063, 1066, 1394, 1397, 1488
PleI GAGTC 2 cut(s) 1550, 2124
PpsI GAGTC 2 cut(s) 1550, 2124
PpuMI RGGWCCY 1 cut(s) 1582
PsiI TTATAA 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 3 cut(s) 202, 1211, 2059
PspGI CCWGG 3 cut(s) 202, 1211, 2059
PspN4I GGNNCC 4 cut(s) 1198, 1583, 1623, 2058
PspPI GGNCC 5 cut(s) 236, 368, 1244, 1582, 1621
PspPPI RGGWCCY 1 cut(s) 1582
PstNI CAGNNNCTG 2 cut(s) 1064, 1067
PsuI RGATCY 3 cut(s) 88, 976, 1900
PsyI GACNNNGTC 2 cut(s) 7, 1317
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 8 cut(s) 553, 898, 1071, 1433, 1727, 1916, 1931, 1971
RsaNI GTAC 8 cut(s) 552, 897, 1070, 1432, 1726, 1915, 1930, 1970
RseI CAYNNNNRTG 2 cut(s) 1096, 1517
SaqAI TTAA 8 cut(s) 831, 966, 1323, 1380, 1491, 1565, 1587, 2102
SatI GCNGC 6 cut(s) 215, 1062, 1065, 1393, 1396, 1487
Sau96I GGNCC 5 cut(s) 236, 368, 1244, 1582, 1621
ScaI AGTACT 3 cut(s) 553, 898, 1916
SchI GAGTC 2 cut(s) 1550, 2124
ScrFI CCNGG 3 cut(s) 204, 1213, 2061
SfaNI GCATC 3 cut(s) 581, 981, 1407
Sfr274I CTCGAG 1 cut(s) 1558
SinI GGWCC 4 cut(s) 236, 368, 1244, 1582
SlaI CTCGAG 1 cut(s) 1558
SmiMI CAYNNNNRTG 2 cut(s) 1096, 1517
SmlI CTYRAG 4 cut(s) 339, 1300, 1558, 1586
SmoI CTYRAG 4 cut(s) 339, 1300, 1558, 1586
SpeI ACTAGT 2 cut(s) 146, 1016
SsiI CCGC 3 cut(s) 361, 1504, 2009
SspMI CTAG 8 cut(s) 66, 147, 270, 533, 1007, 1017, 1607, 2160
StyD4I CCNGG 3 cut(s) 202, 1211, 2059
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 1 cut(s) 1896
TaiI ACGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 1559, 2147
TatI WGTACW 6 cut(s) 551, 896, 1069, 1431, 1725, 1914
TfiI GAWTC 3 cut(s) 404, 1372, 1569
Tru1I TTAA 8 cut(s) 831, 966, 1323, 1380, 1491, 1565, 1587, 2102
Tru9I TTAA 8 cut(s) 831, 966, 1323, 1380, 1491, 1565, 1587, 2102
TscAI CASTG 4 cut(s) 118, 201, 370, 1755
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 6 cut(s) 214, 1061, 1064, 1392, 1395, 1486
Tsp45I GTSAC 1 cut(s) 272
TspGWI ACGGA 2 cut(s) 618, 2074
TspRI CASTG 4 cut(s) 118, 201, 370, 1755
Tth111I GACNNNGTC 2 cut(s) 7, 1317
Vha464I CTTAAG 1 cut(s) 1586
VpaK11BI GGWCC 4 cut(s) 236, 368, 1244, 1582
XapI RAATTY 3 cut(s) 417, 770, 1653
XceI RCATGY 2 cut(s) 1438, 1859
XcmI CCANNNNNNNNNTGG 1 cut(s) 1447
XhoI CTCGAG 1 cut(s) 1558
XmiI GTMKAC 2 cut(s) 572, 2088
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 8 cut(s) 66, 147, 270, 533, 1007, 1017, 1607, 2160
ZrmI AGTACT 3 cut(s) 553, 898, 1916
Zsp2I ATGCAT 1 cut(s) 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.