FvH4_3g32520

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001151
Physical Location & Seq
Reverse (-)
27782714 .. 27785755
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32520.t1

Sequence Viewer

Length: 1716 bp
ATGTATTACACATACGAGGTTATTGATGCTTCTATTCTAACAAGAGTGACAATGACTGAATCGGGATTGGCTCAGCGGCTAGTGGTGAATGAAGGGAGCAGTGAATGGGCAGTTCTGTACACTCTGCAGAACAATCAGTGTGATGTTTATGGCGAGTGTGGGGCAAATGGCATTTGCAAGATTTACAATTCACCGATTTGTGAATGCGTGCAGGGGTTTGTTCCCATATCTCCAAAGGAATGGGAGTTCTCTAACTGGACAAGTGGGTGCACGAGGCGGACAAAACTGCATTGTGAGAAAGGGGAACGTTTTGTGAAGATTAATAATGTGAAACTGCCAGACTTGTTGGATTTTCGGGTGAACAAAAACTTGCTCCGCAGTGGGGATTGTGAGGCAGAGTGTTTAAGGAACTGTTCCTGTGTGGCTTATGCTAATTCAAATATAACCAGTGTAAACAAAGCTTGTCTGATGTGGTTTGGCAAGCTGATTGATATGCGAGAGATTGTTGAACTAGATGGTGACCAGATACATATTCGGATGCCAGCTTCTGAACTAGGTGATACCAGCCAGAGACATAAGAGAATAGTGCTCATCTCAGTCATATCATCAGCAGGTTTTATGCTTCTTTTTCTGGGTTTGTCATGCTGGTATATTTATCTGAAGAAGAGAGAAAAGGAAAGGGGCTCAAGAACCAGTGAGGAGGACTTGGAGTTGCCACTGTTTGATTATGGTACAATAGCAACCGCCACCAATGACTTCTCCGGGACAAACAAACTAGGAGAGGGAGGTTTTGGTCCGGTTTACAAGGCCAAGTTAACACAAGAAGAGTTTGTAGCAGTAAAGAGACTCTCCAGAGATTCTGGGCAAGGTCTTGAAGAGTTTAAGAATGAAGTTACAATGATAGCTAATCTTCAGCACTGGAATCTCGTTAAACTTCTCGGATGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCGAACAAGAGCTTGGACTGTTTTATTTTTGATGAAAACAAGAAGTTGTTGCTGAAGTGGCAAAAGCGGCGAGACATAATAATTGGCATTGCTAGAGGACTTCTCTACCTACATGAAGACTCCAGGCTAAGAATCATTCATAGGGACCTTGAGAGTAGCAACATCTTACTCGACGATGAGTTGAACCCTAAAATTTCTGACTTTGGTATAGCAAGAATTTTCAGACGCAATCAGACCGAAGCTAAAACAAAACGAGTAATTGGGACATATGGATATATGTCCCCGGAGTATGCAATTGATGGGAATTTTTCAGTGAAATCTGATGTCTTCAGCTTTGGAGTGCTTTTGTTAGAGATAGTAAGTGGCAAAAAGAACAGAGGGTTTCATCATCCTGATCACCACCACAATCTTTTGGGACATGTCTGGTTATTGTGGAAACAAAACAAGGGTTTGGAGCTAATGGATATATGCCTGGAGGACTCGTATGTTGAGTTTGAATTGCTAAGGTGCATTCAAGTGGGTCTGTTGTGTGTTCAAAAGCTGCCTAAAGACAGGCCAGTAATGTCATCTGTGGTTCTCATGTTAAGCAATGAGGGAGCAACTTTGCCCCAGCCAAATGAGCCTGGATTCTTTATAGAAAGAGGTTCCATGGACTTTGATAGCTCAAGAGACCAAGGAAAAAGCAACACAGGAAACACAATTACCATTACCACAGAGGAACCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

572

Amino Acids

65.09

Weight (kDa)

6.03

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 2 - 75 6.5e-19 S-locus glycoprotein domain
PAN_2 PF08276 98 - 165 1.2e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 256 - 454 1.1e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 256 - 454 3.8e-43 Protein kinase domain
DUF3403 PF11883 526 - 571 4.1e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 602
AciI CCGC 5 cut(s) 76, 277, 376, 744, 1054
AclI AACGTT 1 cut(s) 307
AcsI RAATTY 3 cut(s) 1179, 1203, 1291
AcuI CTGAAG 4 cut(s) 680, 896, 1061, 1300
AfaI GTAC 3 cut(s) 119, 733, 983
AfiI CCNNNNNNNGG 1 cut(s) 382
AflIII ACRYGT 1 cut(s) 1405
AgsI TTSAA 8 cut(s) 438, 509, 875, 953, 1171, 1484, 1502, 1523
AjnI CCWGG 3 cut(s) 1109, 1458, 1609
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
Alw21I GWGCWC 2 cut(s) 272, 591
Alw26I GTCTC 4 cut(s) 565, 838, 1053, 1650
Alw44I GTGCAC 1 cut(s) 268
AlwNI CAGNNNCTG 1 cut(s) 548
AoxI GGCC 2 cut(s) 807, 1541
ApaLI GTGCAC 1 cut(s) 268
ApeKI GCWGC 2 cut(s) 945, 1528
ApoI RAATTY 3 cut(s) 1179, 1203, 1291
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 794, 1132
AsuC2I CCSGG 2 cut(s) 763, 1271
AsuHPI GGTGA 6 cut(s) 97, 183, 370, 530, 569, 1376
AvaII GGWCC 2 cut(s) 794, 1132
BaeGI GKGCMC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 686
BauI CACGAG 1 cut(s) 271
BbsI GAAGAC 2 cut(s) 1110, 1306
Bbv12I GWGCWC 2 cut(s) 272, 591
BbvI GCAGC 2 cut(s) 932, 1515
BccI CCATC 2 cut(s) 509, 1280
BciT130I CCWGG 3 cut(s) 1111, 1460, 1611
BclI TGATCA 1 cut(s) 1381
BcnI CCSGG 2 cut(s) 763, 1271
BcoDI GTCTC 4 cut(s) 565, 838, 1053, 1650
BfaI CTAG 6 cut(s) 80, 512, 554, 776, 1080, 1710
BfmI CTRYAG 1 cut(s) 125
BfuAI ACCTGC 1 cut(s) 602
BisI GCNGC 4 cut(s) 77, 946, 1055, 1529
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 4 cut(s) 78, 947, 1056, 1530
Bme1390I CCNGG 5 cut(s) 763, 1111, 1271, 1460, 1611
Bme18I GGWCC 2 cut(s) 794, 1132
BmgT120I GGNCC 2 cut(s) 794, 1132
BmiI GGNNCC 3 cut(s) 1133, 1633, 1707
BmrFI CCNGG 5 cut(s) 763, 1111, 1271, 1460, 1611
BmsI GCATC 4 cut(s) 16, 528, 932, 957
BpiI GAAGAC 2 cut(s) 1110, 1306
BplI GAGNNNNNCTC 2 cut(s) 1074, 1106
BpmI CTGGAG 3 cut(s) 835, 1093, 1481
Bpu10I CCTNAGC 1 cut(s) 1490
Bpu1102I GCTNAGC 1 cut(s) 72
BpuEI CTTGAG 3 cut(s) 670, 1157, 1636
BpuMI CCSGG 2 cut(s) 763, 1271
BsaBI GATNNNNATC 1 cut(s) 971
BsaI GGTCTC 1 cut(s) 1650
BsaJI CCNNGG 3 cut(s) 1269, 1635, 1660
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 2 cut(s) 1454, 1484
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 5 cut(s) 260, 447, 693, 923, 1544
Bse3DI GCAATG 2 cut(s) 1074, 1582
Bse8I GATNNNNATC 1 cut(s) 971
BseBI CCWGG 3 cut(s) 1111, 1460, 1611
BseDI CCNNGG 3 cut(s) 1269, 1635, 1660
BseGI GGATG 4 cut(s) 543, 947, 972, 1375
BseJI GATNNNNATC 1 cut(s) 971
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 2 cut(s) 1074, 1582
BseMII CTCAG 2 cut(s) 86, 609
BseNI ACTGG 5 cut(s) 260, 447, 693, 923, 1544
BseRI GAGGAG 1 cut(s) 713
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 2 cut(s) 932, 1515
BseYI CCCAGC 1 cut(s) 1596
BsgI GTGCAG 1 cut(s) 230
BshFI GGCC 2 cut(s) 809, 1543
BsiHKAI GWGCWC 2 cut(s) 272, 591
BsiSI CCGG 3 cut(s) 762, 797, 1271
BslFI GGGAC 5 cut(s) 778, 1145, 1252, 1264, 1416
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 4 cut(s) 565, 838, 1053, 1650
BsmFI GGGAC 5 cut(s) 778, 1145, 1252, 1264, 1416
BsmI GAATGC 2 cut(s) 209, 1497
BsnI GGCC 2 cut(s) 809, 1543
Bso31I GGTCTC 1 cut(s) 1650
Bsp1286I GDGCHC 3 cut(s) 272, 591, 686
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 1 cut(s) 1381
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 1635
BspACI CCGC 5 cut(s) 76, 277, 376, 744, 1054
BspANI GGCC 2 cut(s) 809, 1543
BspCNI CTCAG 2 cut(s) 85, 608
BspLI GGNNCC 3 cut(s) 1133, 1633, 1707
BspMAI CTGCAG 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 1650
BsrDI GCAATG 2 cut(s) 1074, 1582
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 5 cut(s) 260, 447, 693, 923, 1544
BssECI CCNNGG 3 cut(s) 1269, 1635, 1660
BssMI GATC 1 cut(s) 1381
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 2 cut(s) 1635, 1660
Bst2BI CACGAG 1 cut(s) 271
Bst2UI CCWGG 3 cut(s) 1111, 1460, 1611
Bst4CI ACNGT 3 cut(s) 413, 720, 1007
Bst6I CTCTTC 3 cut(s) 659, 819, 870
BstAUI TGTACA 1 cut(s) 117
BstC8I GCNNGC 3 cut(s) 209, 482, 543
BstDEI CTNAG 4 cut(s) 72, 595, 1115, 1490
BstDSI CCRYGG 1 cut(s) 1635
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 4 cut(s) 543, 947, 972, 1375
BstKTI GATC 1 cut(s) 1384
BstMAI GTCTC 4 cut(s) 565, 838, 1053, 1650
BstMBI GATC 1 cut(s) 1381
BstMWI GCNNNNNNNGC 3 cut(s) 1045, 1054, 1606
BstNI CCWGG 3 cut(s) 1111, 1460, 1611
BstNSI RCATGY 2 cut(s) 988, 1409
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 5 cut(s) 761, 1109, 1269, 1458, 1609
BstSFI CTRYAG 1 cut(s) 125
BstSLI GKGCMC 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 932, 1515
BstV2I GAAGAC 2 cut(s) 1110, 1306
BstXI CCANNNNNNTGG 1 cut(s) 240
BsuRI GGCC 2 cut(s) 809, 1543
BtgI CCRYGG 1 cut(s) 1635
BtsCI GGATG 4 cut(s) 543, 947, 972, 1375
BtsI GCAGTG 2 cut(s) 106, 385
BtsIMutI CAGTG 8 cut(s) 106, 143, 385, 454, 700, 716, 916, 1305
BveI ACCTGC 1 cut(s) 602
Cac8I GCNNGC 3 cut(s) 209, 482, 543
CaiI CAGNNNCTG 1 cut(s) 548
Cfr13I GGNCC 2 cut(s) 794, 1132
CseI GACGC 1 cut(s) 1221
Csp6I GTAC 3 cut(s) 118, 732, 982
CviAII CATG 6 cut(s) 642, 985, 1100, 1406, 1567, 1636
CviQI GTAC 3 cut(s) 118, 732, 982
DdeI CTNAG 4 cut(s) 72, 595, 1115, 1490
DpnI GATC 1 cut(s) 1383
DpnII GATC 1 cut(s) 1381
Eam1104I CTCTTC 3 cut(s) 659, 819, 870
EarI CTCTTC 3 cut(s) 659, 819, 870
EciI GGCGGA 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 1635, 1660
Eco24I GRGCYC 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 1650
Eco47I GGWCC 2 cut(s) 794, 1132
Eco57I CTGAAG 4 cut(s) 680, 896, 1061, 1300
Eco91I GGTNACC 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 1132
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 3 cut(s) 1109, 1458, 1609
EcoT14I CCWWGG 2 cut(s) 1635, 1660
EcoT38I GRGCYC 1 cut(s) 686
ErhI CCWWGG 2 cut(s) 1635, 1660
FaeI CATG 6 cut(s) 645, 988, 1103, 1409, 1570, 1639
FaqI GGGAC 5 cut(s) 778, 1145, 1252, 1264, 1416
FatI CATG 6 cut(s) 641, 984, 1099, 1405, 1566, 1635
FauNDI CATATG 1 cut(s) 1255
FbaI TGATCA 1 cut(s) 1381
Fnu4HI GCNGC 4 cut(s) 77, 946, 1055, 1529
FokI GGATG 4 cut(s) 550, 954, 979, 1362
FriOI GRGCYC 1 cut(s) 686
Fsp4HI GCNGC 4 cut(s) 77, 946, 1055, 1529
FspBI CTAG 6 cut(s) 80, 512, 554, 776, 1080, 1710
GluI GCNGC 4 cut(s) 77, 946, 1055, 1529
GsaI CCCAGC 1 cut(s) 1600
GsuI CTGGAG 3 cut(s) 835, 1093, 1481
HaeIII GGCC 2 cut(s) 809, 1543
HapII CCGG 3 cut(s) 762, 797, 1271
HgaI GACGC 1 cut(s) 1221
Hin1II CATG 6 cut(s) 645, 988, 1103, 1409, 1570, 1639
HincII GTYRAC 1 cut(s) 816
HindII GTYRAC 1 cut(s) 816
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 8 cut(s) 59, 846, 857, 922, 1106, 1119, 1466, 1614
HpaI GTTAAC 1 cut(s) 816
HpaII CCGG 3 cut(s) 762, 797, 1271
HphI GGTGA 6 cut(s) 97, 183, 370, 530, 569, 1376
Hpy166II GTNNAC 6 cut(s) 120, 270, 361, 454, 802, 816
Hpy188I TCNGA 9 cut(s) 468, 537, 550, 660, 941, 1186, 1211, 1221, 1309
Hpy188III TCNNGA 6 cut(s) 63, 687, 852, 872, 1379, 1653
Hpy8I GTNNAC 6 cut(s) 120, 270, 361, 454, 802, 816
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 2 cut(s) 86, 947
HpyCH4III ACNGT 3 cut(s) 413, 720, 1007
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 8 cut(s) 127, 177, 211, 270, 289, 948, 1280, 1497
HpyF10VI GCNNNNNNNGC 3 cut(s) 1045, 1054, 1606
HpyF3I CTNAG 4 cut(s) 72, 595, 1115, 1490
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 6 cut(s) 645, 988, 1103, 1409, 1570, 1639
Ksp22I TGATCA 1 cut(s) 1381
KspAI GTTAAC 1 cut(s) 816
Kzo9I GATC 1 cut(s) 1381
LmnI GCTCC 4 cut(s) 96, 378, 1441, 1583
Lsp1109I GCAGC 2 cut(s) 932, 1515
LweI GCATC 4 cut(s) 16, 528, 932, 957
MaeI CTAG 6 cut(s) 80, 512, 554, 776, 1080, 1710
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 3 cut(s) 46, 518, 892
MalI GATC 1 cut(s) 1383
MboI GATC 1 cut(s) 1381
MboII GAAGA 9 cut(s) 328, 673, 676, 836, 887, 902, 971, 1115, 1306
MfeI CAATTG 1 cut(s) 1281
MhlI GDGCHC 3 cut(s) 272, 591, 686
MlyI GAGTC 3 cut(s) 840, 1100, 1460
MmeI TCCRAC 1 cut(s) 327
MseI TTAA 6 cut(s) 321, 404, 815, 882, 930, 1571
MslI CAYNNNNRTG 1 cut(s) 1502
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 3 cut(s) 762, 797, 1271
MspR9I CCNGG 5 cut(s) 763, 1111, 1271, 1460, 1611
MunI CAATTG 1 cut(s) 1281
Mva1269I GAATGC 2 cut(s) 209, 1497
MvaI CCWGG 3 cut(s) 1111, 1460, 1611
MwoI GCNNNNNNNGC 3 cut(s) 1045, 1054, 1606
NciI CCSGG 2 cut(s) 763, 1271
NcoI CCATGG 1 cut(s) 1635
NdeI CATATG 1 cut(s) 1255
NdeII GATC 1 cut(s) 1381
NlaIII CATG 6 cut(s) 645, 988, 1103, 1409, 1570, 1639
NlaIV GGNNCC 3 cut(s) 1133, 1633, 1707
NmuCI GTSAC 2 cut(s) 46, 518
NspI RCATGY 2 cut(s) 988, 1409
PciI ACATGT 1 cut(s) 1405
PctI GAATGC 2 cut(s) 209, 1497
PfeI GAWTC 5 cut(s) 59, 857, 922, 1119, 1614
PfoI TCCNGGA 1 cut(s) 761
PkrI GCNGC 4 cut(s) 78, 947, 1056, 1530
PleI GAGTC 3 cut(s) 840, 1100, 1460
PpsI GAGTC 3 cut(s) 840, 1100, 1460
PpuMI RGGWCCY 1 cut(s) 1132
PscI ACATGT 1 cut(s) 1405
PshBI ATTAAT 1 cut(s) 321
Psp1406I AACGTT 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 1132
Psp6I CCWGG 3 cut(s) 1109, 1458, 1609
PspEI GGTNACC 1 cut(s) 518
PspFI CCCAGC 1 cut(s) 1596
PspGI CCWGG 3 cut(s) 1109, 1458, 1609
PspN4I GGNNCC 3 cut(s) 1133, 1633, 1707
PspPI GGNCC 2 cut(s) 794, 1132
PspPPI RGGWCCY 1 cut(s) 1132
PstI CTGCAG 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 548
RsaI GTAC 3 cut(s) 119, 733, 983
RsaNI GTAC 3 cut(s) 118, 732, 982
RseI CAYNNNNRTG 1 cut(s) 1502
SaqAI TTAA 6 cut(s) 321, 404, 815, 882, 930, 1571
SatI GCNGC 4 cut(s) 77, 946, 1055, 1529
Sau3AI GATC 1 cut(s) 1381
Sau96I GGNCC 2 cut(s) 794, 1132
SchI GAGTC 3 cut(s) 840, 1100, 1460
ScrFI CCNGG 5 cut(s) 763, 1111, 1271, 1460, 1611
SduI GDGCHC 3 cut(s) 272, 591, 686
SfaNI GCATC 4 cut(s) 16, 528, 932, 957
SfcI CTRYAG 1 cut(s) 125
SinI GGWCC 2 cut(s) 794, 1132
SmiMI CAYNNNNRTG 1 cut(s) 1502
SmlI CTYRAG 3 cut(s) 685, 1136, 1651
SmoI CTYRAG 3 cut(s) 685, 1136, 1651
SsiI CCGC 5 cut(s) 76, 277, 376, 744, 1054
SspMI CTAG 6 cut(s) 80, 512, 554, 776, 1080, 1710
StyD4I CCNGG 5 cut(s) 761, 1109, 1269, 1458, 1609
StyI CCWWGG 2 cut(s) 1635, 1660
TaaI ACNGT 3 cut(s) 413, 720, 1007
TaiI ACGT 1 cut(s) 310
TaqI TCGA 1 cut(s) 1158
TaqII GACCGA 1 cut(s) 1238
TatI WGTACW 2 cut(s) 117, 981
TauI GCSGC 2 cut(s) 79, 1057
TfiI GAWTC 5 cut(s) 59, 857, 922, 1119, 1614
Tru1I TTAA 6 cut(s) 321, 404, 815, 882, 930, 1571
Tru9I TTAA 6 cut(s) 321, 404, 815, 882, 930, 1571
TscAI CASTG 8 cut(s) 106, 143, 385, 454, 700, 723, 923, 1305
TseFI GTSAC 2 cut(s) 46, 518
TseI GCWGC 2 cut(s) 945, 1528
Tsp45I GTSAC 2 cut(s) 46, 518
TspDTI ATGAA 6 cut(s) 105, 903, 1035, 1115, 1116, 1361
TspRI CASTG 8 cut(s) 106, 143, 385, 454, 700, 723, 923, 1305
VneI GTGCAC 1 cut(s) 268
VpaK11BI GGWCC 2 cut(s) 794, 1132
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 3 cut(s) 1179, 1203, 1291
XceI RCATGY 2 cut(s) 988, 1409
XspI CTAG 6 cut(s) 80, 512, 554, 776, 1080, 1710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.