MD03G1186000.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001151
Physical Location & Seq
Reverse (-)
25353634 .. 25354728
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1186000.v1.1.491

Sequence Viewer

Length: 750 bp
ATGCTGATTTATGAGTACATGCCCAACAAAAGCTTGGATTGCTACATTTTCGATCAAAATAGAAAGGTGTTGTTGAACTGGAAAAAGCGATTCGACATTATCATGGGAATCGCACGAGGACTTCTCTACCTCCACCAAGACTCTAGACTAAGAATCATTCATAGGGACCTTAAGAGCAGCAACATCTTACTTGACGATGAGTTGAACCCCAAAATATCTGACTTTGGCATAGCAAGGATTTTCGAACGCAATCAAACTGAAGCAAAAACAAAACGCGTAATTGGGACGTACGGATATATGTCTCCTGAGTATGCAATTGACGGGAAGTTTTCAGTGAAATCTGATGTCTTCAGTTTTGGAGTTCTTTTGTTGGAGTTAATAAGTGGCAGAAAGAACAGAGGGTTTCACCATCCAGATCACGATCACAATCTTCTTGGACATGCATGGTTACTATGGAACAAAGGCAAGTGTTCGGAACTAATAGATCAATGCTTAGAGTGCTCGTACGTTGAGTTTGAGGTGCAAAGATGCATTCAAGTAGGTCTATTATGTGTTCAAAAGCTTCCGGTAGACAGACCGGCAATGTCATCAGTGGTGTTCATGTTAGGCAATGAGGGAGCGCCATTGCCTCAACCGAAGGAGCCTGGTTTCTTTACTGAAAGAAGCTCCGAGGACATCGATACCTTAGTCTACGAAGGTAGAAGCCAGTCGGGAAGCTCAATTACCATTACCACGGTGGTAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

250

Amino Acids

28.54

Weight (kDa)

7.04

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 198 1.5e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 132 7.3e-29 Protein kinase domain
DUF3403 PF11883 204 - 249 4.7e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 570, 690
AccII CGCG 1 cut(s) 276
AcuI CTGAAG 2 cut(s) 279, 334
AfaI GTAC 3 cut(s) 17, 290, 506
AflII CTTAAG 1 cut(s) 170
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 4 cut(s) 76, 205, 536, 557
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 5 cut(s) 33, 562, 666, 717, 743
AluI AGCT 5 cut(s) 33, 562, 666, 717, 743
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 1 cut(s) 306
ApeKI GCWGC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 398
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BarI GAAGNNNNNNTAC 2 cut(s) 706, 738
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 417
BciT130I CCWGG 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 2 cut(s) 144, 744
BfoI RGCGCY 1 cut(s) 623
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 167, 642
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 108, 140
Bpu14I TTCGAA 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 678
BsaBI GATNNNNATC 2 cut(s) 420, 426
BsaJI CCNNGG 2 cut(s) 669, 732
BsaWI WCCGGW 1 cut(s) 565
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 83, 706
Bse3DI GCAATG 3 cut(s) 588, 616, 623
Bse8I GATNNNNATC 2 cut(s) 420, 426
BseBI CCWGG 1 cut(s) 645
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 669, 732
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 2 cut(s) 420, 426
BseMI GCAATG 3 cut(s) 588, 616, 623
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 83, 706
BseXI GCAGC 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 678
BsiHKAI GWGCWC 1 cut(s) 503
BsiSI CCGG 2 cut(s) 566, 578
BsiWI CGTACG 2 cut(s) 288, 504
BslFI GGGAC 2 cut(s) 179, 298
BsmAI GTCTC 1 cut(s) 306
BsmFI GGGAC 2 cut(s) 179, 298
BsmI GAATGC 1 cut(s) 531
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 4 cut(s) 52, 415, 421, 484
BspCNI CTCAG 1 cut(s) 298
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 167, 642
BspT104I TTCGAA 1 cut(s) 243
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 3 cut(s) 588, 616, 623
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 2 cut(s) 83, 706
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 2 cut(s) 669, 732
BssMI GATC 4 cut(s) 52, 415, 421, 484
BssSI CACGAG 1 cut(s) 114
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 1 cut(s) 736
BstAFI CTTAAG 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 243
BstDEI CTNAG 4 cut(s) 149, 306, 493, 685
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 4 cut(s) 55, 418, 424, 487
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 4 cut(s) 52, 415, 421, 484
BstMWI GCNNNNNNNGC 2 cut(s) 39, 498
BstNI CCWGG 1 cut(s) 645
BstNSI RCATGY 2 cut(s) 22, 443
BstSCI CCNGG 1 cut(s) 643
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 678
BsuTUI ATCGAT 1 cut(s) 678
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 339, 597
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 678
Csp6I GTAC 3 cut(s) 16, 289, 505
CviAII CATG 5 cut(s) 19, 103, 440, 444, 601
CviJI RGCY 7 cut(s) 33, 562, 643, 666, 705, 717, 743
CviKI_1 RGCY 7 cut(s) 33, 562, 643, 666, 705, 717, 743
CviQI GTAC 3 cut(s) 16, 289, 505
DdeI CTNAG 4 cut(s) 149, 306, 493, 685
DpnI GATC 4 cut(s) 54, 417, 423, 486
DpnII GATC 4 cut(s) 52, 415, 421, 484
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 279, 334
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 643
EcoT22I ATGCAT 2 cut(s) 445, 533
FaeI CATG 5 cut(s) 22, 106, 443, 447, 604
FaqI GGGAC 2 cut(s) 179, 298
FatI CATG 5 cut(s) 18, 102, 439, 443, 600
FblI GTMKAC 2 cut(s) 570, 690
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 2 cut(s) 144, 744
GlaI GCGC 1 cut(s) 621
GluI GCNGC 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 623
HapII CCGG 2 cut(s) 566, 578
HhaI GCGC 1 cut(s) 622
Hin1II CATG 5 cut(s) 22, 106, 443, 447, 604
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HindIII AAGCTT 2 cut(s) 31, 560
HinfI GANTC 4 cut(s) 90, 108, 140, 153
HpaII CCGG 2 cut(s) 566, 578
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 571, 691
Hpy188I TCNGA 4 cut(s) 220, 343, 475, 670
Hpy188III TCNNGA 5 cut(s) 144, 305, 413, 419, 711
Hpy8I GTNNAC 2 cut(s) 571, 691
HpyAV CCTTC 2 cut(s) 631, 689
HpyCH4III ACNGT 1 cut(s) 736
HpyCH4IV ACGT 2 cut(s) 287, 507
HpyCH4V TGCA 4 cut(s) 314, 443, 523, 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 498
HpyF3I CTNAG 4 cut(s) 149, 306, 493, 685
HpySE526I ACGT 2 cut(s) 287, 507
Hsp92II CATG 5 cut(s) 22, 106, 443, 447, 604
HspAI GCGC 1 cut(s) 620
Kzo9I GATC 4 cut(s) 52, 415, 421, 484
LmnI GCTCC 3 cut(s) 617, 640, 671
LpnPI CCDG 8 cut(s) 64, 318, 426, 579, 591, 630, 657, 719
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 518
MaeI CTAG 2 cut(s) 144, 744
MaeII ACGT 2 cut(s) 287, 507
MaeIII GTNAC 1 cut(s) 447
MalI GATC 4 cut(s) 54, 417, 423, 486
MboI GATC 4 cut(s) 52, 415, 421, 484
MboII GAAGA 2 cut(s) 340, 422
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 3 cut(s) 279, 315, 720
MluI ACGCGT 1 cut(s) 274
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 7 cut(s) 110, 140, 392, 511, 607, 639, 664
Mph1103I ATGCAT 2 cut(s) 445, 533
MseI TTAA 2 cut(s) 171, 377
MslI CAYNNNNRTG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 2 cut(s) 566, 578
MspR9I CCNGG 1 cut(s) 645
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 531
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 2 cut(s) 39, 498
NdeII GATC 4 cut(s) 52, 415, 421, 484
NlaIII CATG 5 cut(s) 22, 106, 443, 447, 604
NlaIV GGNNCC 2 cut(s) 167, 642
NsiI ATGCAT 2 cut(s) 445, 533
NspI RCATGY 2 cut(s) 22, 443
NspV TTCGAA 1 cut(s) 243
PctI GAATGC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 90, 108, 153
Pfl23II CGTACG 2 cut(s) 288, 504
PkrI GCNGC 1 cut(s) 179
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspLI CGTACG 2 cut(s) 288, 504
PspN4I GGNNCC 2 cut(s) 167, 642
PspPI GGNCC 1 cut(s) 166
PspPPI RGGWCCY 1 cut(s) 166
RsaI GTAC 3 cut(s) 17, 290, 506
RsaNI GTAC 3 cut(s) 16, 289, 505
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 2 cut(s) 171, 377
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 4 cut(s) 52, 415, 421, 484
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 518
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 3 cut(s) 279, 315, 720
SspMI CTAG 2 cut(s) 144, 744
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 736
TaiI ACGT 2 cut(s) 290, 510
TaqI TCGA 4 cut(s) 51, 93, 243, 678
TasI AATT 3 cut(s) 279, 315, 720
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 3 cut(s) 90, 108, 153
Tru1I TTAA 2 cut(s) 171, 377
Tru9I TTAA 2 cut(s) 171, 377
TscAI CASTG 2 cut(s) 339, 597
TseI GCWGC 1 cut(s) 177
TspDTI ATGAA 2 cut(s) 149, 589
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 2 cut(s) 339, 597
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 166
XbaI TCTAGA 1 cut(s) 143
XceI RCATGY 2 cut(s) 22, 443
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 733
XmiI GTMKAC 2 cut(s) 570, 690
XspI CTAG 2 cut(s) 144, 744
Zsp2I ATGCAT 2 cut(s) 445, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.