Rroxscaffold_1G00044450

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001151
Physical Location & Seq
Reverse (-)
63419757 .. 63428135
8379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044450.1

Sequence Viewer

Length: 1245 bp
ATGTGGTTTAAAGACCTTATTGATATCCGTGAGTTTGATGATGAAAGTGAGCAACATATTTATATTAGGATGCCAGCTTCAGAACTAGGTCATGCCGGTCAGAAGGAGAAAAGAATAGTGCTCATAGTGGTCATCTCAGCAGCTTGTGTTCTAATTTTGCTGGGTGGGTCATGTTGGCTTATAATTTGGGAGAAGAAAGAAAGGAAGAGGGGTTCAAGATCGAACAAGGAAGACATGGATTTGCCACTGTTTGATTTTCATATAATTGCAGCAGGCACCAATAACTTCTCAAGGAAAAACATACTTGGAGAGGGAGGTTTTGGTCCAGTTTACAAGGCCAACTTAGCACAAGAACAACTAGTAGCAGTAAAAAGACTCTCGAAAGATTCCAGGCAAGGTATTGATGAGTTTAAGAATGAAGCTACGATGATAGCCAACCTTCAACACAGGAATCTTGTTAAACTTTTGGGCTGCTGCATTGAAGGACAAGAGAGGATGCTAATCTATGAGTATATGCCTAACAAAAGCTTGGACTGCTTTATTTTCGATGAAAATAGAAAGAAATCGCTCGATTGGCAAAAACGAGTCAACATTATAATTGGAACTGCACGAGGACTTCTCTACCTCCACCAAGACTCCAGATTGAGAATCATTCATAGGGACCTCAAGAGTAGCAACATTTTACTCGACAATGAGCTGAACCCCAAAATATCTGACTTCGGCATAGCAAGAATTTTTGGACTTGATCAGACTGAAGCAAAGACAAAACGAGTGATTGGCACATATGGGTATATGTCTCCCGAGTACGTGATTGATGGGCACTTTTCAGTGAAATCTGATGTCTTTAGTTTTGGAGTGCTTTTGCTAGAGATGGTAAGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGATCACCATCACAGTCTTCTGGGACATGCCTGGCTACTATGGAACAAAGAAAAGGTTTTGGAACTATTAGATCCATGCTTGGAGGACTCATATGTTGAGTTTGAGGTACTAAGATGTATACAAGTGGGTCTATTATGCGTGCAAGAGCGGCCCATCGACAGACCAGTAATGTCATCAGTGGTGTTCATGTTAAGCAACGAGGGAGCAATGTTGCCTGAACCGAAGGAGCCTGGTTTCTTCACAGAACGAAGTTCCATGGATGGTGATACCTTCATTAGTGAAGGAAGAAGCAAAACAGGAAACATGATTACCATTACCACGATGGAAGCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

414

Amino Acids

47.41

Weight (kDa)

6.52

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 97 - 296 2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 99 - 297 8.3e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 370 - 414 3.5e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 182, 596
AccB1I GGYRCC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 1027
AciI CCGC 1 cut(s) 1057
AclWI GGATC 1 cut(s) 974
AcsI RAATTY 1 cut(s) 732
AcuI CTGAAG 2 cut(s) 63, 774
AfaI GTAC 2 cut(s) 806, 1017
AgsI TTSAA 3 cut(s) 216, 443, 482
AhlI ACTAGT 1 cut(s) 358
AjnI CCWGG 3 cut(s) 389, 938, 1138
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 6 cut(s) 77, 143, 422, 528, 697, 1238
AluI AGCT 6 cut(s) 77, 143, 422, 528, 697, 1238
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 801
AlwI GGATC 1 cut(s) 974
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 2 cut(s) 336, 1058
ApeKI GCWGC 4 cut(s) 140, 269, 471, 474
ApoI RAATTY 1 cut(s) 732
AspS9I GGNCC 3 cut(s) 323, 661, 1059
AsuHPI GGTGA 2 cut(s) 905, 1184
AvaI CYCGRG 1 cut(s) 800
AvaII GGWCC 2 cut(s) 323, 661
BaeGI GKGCMC 1 cut(s) 822
BanI GGYRCC 1 cut(s) 275
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 2 cut(s) 237, 917
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 152, 281, 458, 461
BccI CCATC 6 cut(s) 809, 865, 924, 1070, 1163, 1225
BciT130I CCWGG 3 cut(s) 391, 940, 1140
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 1 cut(s) 801
BcuI ACTAGT 1 cut(s) 358
BfaI CTAG 3 cut(s) 86, 359, 866
BisI GCNGC 5 cut(s) 141, 270, 472, 475, 1058
BlsI GCNGC 5 cut(s) 142, 271, 473, 476, 1059
Bme1390I CCNGG 3 cut(s) 391, 940, 1140
Bme18I GGWCC 2 cut(s) 323, 661
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 3 cut(s) 323, 661, 1059
BmiI GGNNCC 3 cut(s) 277, 662, 1137
BmrFI CCNGG 3 cut(s) 391, 940, 1140
BmsI GCATC 2 cut(s) 60, 486
BpiI GAAGAC 2 cut(s) 237, 917
BplI GAGNNNNNCTC 2 cut(s) 603, 635
BpmI CTGGAG 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 274, 650
BsaAI YACGTR 1 cut(s) 808
BsaBI GATNNNNATC 2 cut(s) 500, 915
BsaJI CCNNGG 1 cut(s) 1164
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 326, 1073
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 2 cut(s) 500, 915
BseBI CCWGG 3 cut(s) 391, 940, 1140
BseDI CCNNGG 1 cut(s) 1164
BseGI GGATG 4 cut(s) 75, 501, 904, 1174
BseJI GATNNNNATC 2 cut(s) 500, 915
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 2 cut(s) 326, 1073
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 4 cut(s) 152, 281, 458, 461
BseYI CCCAGC 1 cut(s) 160
BsgI GTGCAG 1 cut(s) 591
BshFI GGCC 2 cut(s) 338, 1060
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 674, 945
BsmAI GTCTC 1 cut(s) 801
BsmFI GGGAC 2 cut(s) 674, 945
BsnI GGCC 2 cut(s) 338, 1060
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 2 cut(s) 123, 822
Bsp143I GATC 4 cut(s) 218, 745, 910, 979
Bsp19I CCATGG 1 cut(s) 1164
BspACI CCGC 1 cut(s) 1057
BspANI GGCC 2 cut(s) 338, 1060
BspCNI CTCAG 1 cut(s) 149
BspLI GGNNCC 3 cut(s) 277, 662, 1137
BspPI GGATC 1 cut(s) 974
BspT107I GGYRCC 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 1122
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 2 cut(s) 326, 1073
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 1164
BssMI GATC 4 cut(s) 218, 745, 910, 979
BssNAI GTATAC 1 cut(s) 1028
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 1164
Bst1107I GTATAC 1 cut(s) 1028
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 3 cut(s) 391, 940, 1140
Bst4CI ACNGT 2 cut(s) 249, 923
Bst6I CTCTTC 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 3 cut(s) 75, 274, 1049
BstDEI CTNAG 3 cut(s) 136, 343, 1019
BstDSI CCRYGG 1 cut(s) 1164
BstF5I GGATG 4 cut(s) 75, 501, 904, 1174
BstKTI GATC 4 cut(s) 221, 748, 913, 982
BstMAI GTCTC 1 cut(s) 801
BstMBI GATC 4 cut(s) 218, 745, 910, 979
BstMWI GCNNNNNNNGC 4 cut(s) 344, 534, 574, 1057
BstNI CCWGG 3 cut(s) 391, 940, 1140
BstNSI RCATGY 1 cut(s) 938
BstSCI CCNGG 3 cut(s) 389, 938, 1138
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 4 cut(s) 152, 281, 458, 461
BstV2I GAAGAC 2 cut(s) 237, 917
BstX2I RGATCY 1 cut(s) 979
BstYI RGATCY 1 cut(s) 979
BstZ17I GTATAC 1 cut(s) 1028
BsuRI GGCC 2 cut(s) 338, 1060
BtgI CCRYGG 1 cut(s) 1164
BtsCI GGATG 4 cut(s) 75, 501, 904, 1174
BtsIMutI CAGTG 3 cut(s) 245, 834, 1092
Cac8I GCNNGC 3 cut(s) 75, 274, 1049
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 3 cut(s) 323, 661, 1059
Csp6I GTAC 2 cut(s) 805, 1016
CviAII CATG 8 cut(s) 92, 171, 235, 935, 984, 1096, 1165, 1213
CviQI GTAC 2 cut(s) 805, 1016
DdeI CTNAG 3 cut(s) 136, 343, 1019
DpnI GATC 4 cut(s) 220, 747, 912, 981
DpnII GATC 4 cut(s) 218, 745, 910, 979
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 1164
Eco32I GATATC 1 cut(s) 25
Eco47I GGWCC 2 cut(s) 323, 661
Eco57I CTGAAG 2 cut(s) 63, 774
Eco88I CYCGRG 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 661
EcoRII CCWGG 3 cut(s) 389, 938, 1138
EcoRV GATATC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 1164
ErhI CCWWGG 1 cut(s) 1164
FaeI CATG 8 cut(s) 95, 174, 238, 938, 987, 1099, 1168, 1216
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 674, 945
FatI CATG 8 cut(s) 91, 170, 234, 934, 983, 1095, 1164, 1212
FauNDI CATATG 2 cut(s) 784, 1000
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 5 cut(s) 141, 270, 472, 475, 1058
FokI GGATG 4 cut(s) 82, 508, 891, 1181
Fsp4HI GCNGC 5 cut(s) 141, 270, 472, 475, 1058
FspBI CTAG 3 cut(s) 86, 359, 866
GluI GCNGC 5 cut(s) 141, 270, 472, 475, 1058
GsaI CCCAGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 622
HaeIII GGCC 2 cut(s) 338, 1060
HapII CCGG 1 cut(s) 96
Hin1II CATG 8 cut(s) 95, 174, 238, 938, 987, 1099, 1168, 1216
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 526
HinfI GANTC 7 cut(s) 375, 386, 451, 585, 635, 648, 995
HpaII CCGG 1 cut(s) 96
HphI GGTGA 2 cut(s) 905, 1184
Hpy166II GTNNAC 3 cut(s) 331, 589, 1028
Hpy188I TCNGA 5 cut(s) 82, 102, 715, 750, 838
Hpy188III TCNNGA 6 cut(s) 216, 379, 639, 667, 800, 908
Hpy8I GTNNAC 3 cut(s) 331, 589, 1028
HpyAV CCTTC 6 cut(s) 97, 449, 476, 1126, 1184, 1189
HpyCH4III ACNGT 2 cut(s) 249, 923
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 4 cut(s) 269, 477, 608, 1051
HpyF10VI GCNNNNNNNGC 4 cut(s) 344, 534, 574, 1057
HpyF3I CTNAG 3 cut(s) 136, 343, 1019
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 8 cut(s) 95, 174, 238, 938, 987, 1099, 1168, 1216
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 4 cut(s) 218, 745, 910, 979
LmnI GCTCC 2 cut(s) 1112, 1135
Lsp1109I GCAGC 4 cut(s) 152, 281, 458, 461
LweI GCATC 2 cut(s) 60, 486
MaeI CTAG 3 cut(s) 86, 359, 866
MaeII ACGT 1 cut(s) 807
MalI GATC 4 cut(s) 220, 747, 912, 981
MbiI CCGCTC 1 cut(s) 1057
MboI GATC 4 cut(s) 218, 745, 910, 979
MboII GAAGA 6 cut(s) 205, 217, 242, 917, 1138, 1206
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 2 cut(s) 123, 822
MluCI AATT 5 cut(s) 153, 183, 264, 597, 732
MlyI GAGTC 4 cut(s) 369, 594, 629, 989
MseI TTAA 4 cut(s) 9, 411, 459, 1100
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 3 cut(s) 391, 940, 1140
MvaI CCWGG 3 cut(s) 391, 940, 1140
MwoI GCNNNNNNNGC 4 cut(s) 344, 534, 574, 1057
NcoI CCATGG 1 cut(s) 1164
NdeI CATATG 2 cut(s) 784, 1000
NdeII GATC 4 cut(s) 218, 745, 910, 979
NlaIII CATG 8 cut(s) 95, 174, 238, 938, 987, 1099, 1168, 1216
NlaIV GGNNCC 3 cut(s) 277, 662, 1137
NspI RCATGY 1 cut(s) 938
PfeI GAWTC 3 cut(s) 386, 451, 648
PkrI GCNGC 5 cut(s) 142, 271, 473, 476, 1059
PleI GAGTC 4 cut(s) 369, 593, 629, 989
PpsI GAGTC 4 cut(s) 369, 593, 629, 989
Ppu21I YACGTR 1 cut(s) 808
PpuMI RGGWCCY 1 cut(s) 661
PsiI TTATAA 2 cut(s) 182, 596
Psp5II RGGWCCY 1 cut(s) 661
Psp6I CCWGG 3 cut(s) 389, 938, 1138
PspFI CCCAGC 1 cut(s) 160
PspGI CCWGG 3 cut(s) 389, 938, 1138
PspN4I GGNNCC 3 cut(s) 277, 662, 1137
PspPI GGNCC 3 cut(s) 323, 661, 1059
PspPPI RGGWCCY 1 cut(s) 661
PsuI RGATCY 1 cut(s) 979
RsaI GTAC 2 cut(s) 806, 1017
RsaNI GTAC 2 cut(s) 805, 1016
SaqAI TTAA 4 cut(s) 9, 411, 459, 1100
SatI GCNGC 5 cut(s) 141, 270, 472, 475, 1058
Sau3AI GATC 4 cut(s) 218, 745, 910, 979
Sau96I GGNCC 3 cut(s) 323, 661, 1059
SchI GAGTC 4 cut(s) 369, 594, 629, 989
ScrFI CCNGG 3 cut(s) 391, 940, 1140
SduI GDGCHC 2 cut(s) 123, 822
SfaNI GCATC 2 cut(s) 60, 486
SinI GGWCC 2 cut(s) 323, 661
SmlI CTYRAG 2 cut(s) 289, 665
SmoI CTYRAG 2 cut(s) 289, 665
SpeI ACTAGT 1 cut(s) 358
Sse9I AATT 5 cut(s) 153, 183, 264, 597, 732
SsiI CCGC 1 cut(s) 1057
SspMI CTAG 3 cut(s) 86, 359, 866
StyD4I CCNGG 3 cut(s) 389, 938, 1138
StyI CCWWGG 1 cut(s) 1164
TaaI ACNGT 2 cut(s) 249, 923
TaiI ACGT 1 cut(s) 810
TaqI TCGA 7 cut(s) 221, 380, 546, 570, 687, 1065, 1240
TasI AATT 5 cut(s) 153, 183, 264, 597, 732
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 3 cut(s) 386, 451, 648
Tru1I TTAA 4 cut(s) 9, 411, 459, 1100
Tru9I TTAA 4 cut(s) 9, 411, 459, 1100
TscAI CASTG 3 cut(s) 252, 834, 1092
TseI GCWGC 4 cut(s) 140, 269, 471, 474
TspDTI ATGAA 8 cut(s) 57, 248, 432, 564, 644, 890, 1084, 1171
TspGWI ACGGA 1 cut(s) 17
TspRI CASTG 3 cut(s) 252, 834, 1092
VpaK11BI GGWCC 2 cut(s) 323, 661
XapI RAATTY 1 cut(s) 732
XceI RCATGY 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 1228
XmiI GTMKAC 1 cut(s) 1027
XspI CTAG 3 cut(s) 86, 359, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.