Rw5G022850

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001151
Physical Location & Seq
Forward (+)
31848694 .. 31852421
3728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022850.1

Sequence Viewer

Length: 2526 bp
ATGGTGTCTTTAGTGGGAGAATTACCACCCAATAAAGAGTATGATGCAATAAGTCGAAAACTACTGGTGTCCATCTCCTTTCCATTCCTTAGCCGAAAAATTCAGATGAAGGGCCTTCCTTTCATCTCATTCATCTCTCTTTTCCATGTTTTAGTGTTCAGTTTATGCAGTGCAGTTGATACTATATCTCCAACACAATCCATCACCAGCTCCAATACCTTGGTTTCTTCCGGTGGAAGATTTGAATTAGGACTCTTCTCCTCCGGAAACTCTAAAGCATGGTATTTAGGAATTTGGTACACCAAGTTTTCAAATATAACCGTATGGGTCGCCAACAGAGAAAACCCAATTCCAGGTTCAGATGGAGCCCTCACATTAACCGAAAATGGTTCTTTGGTCCTTTTAGACCAGATGAACAACACTGTATGGTATTCTAATTTTTCCACAGTACTTGAAGATCCTGTTGCACAGCTTTTGGAGACTGGAAATTTTGTTGTCAGAGACAAGGCTACAGCAGGTTCGGAAAGTTTTGTATGGCAGAGTTTTGATTCCCCATCGGATACATTGTTAGCAGGGATGAGGCTTGGATGGGATTTCAGAACCGGGCTCAACAAGTTCTTGACTTCTTGGAAAAATGCTAGTGATCCATCTCTTGGTGAGTATACCTATAGGATTGTTAATCTTGCTTTGCCTCAGCTTGTTCTTGCTAAGGGATCGGAGAAAGAGTTTCGAAGTGGGCCCTGGAATGGTATTCGGTTTACTGGTTATCCTGTTTCGAACAATGAAATTATAACACCAAGTTATGTGTATGATACAAATGAGTTGTATTACACGTATAATGACAATTCTCTTATTACAAGATTGCAGCTGACTGAAGGAGGTGACATGCAGCGGTTAGTGCTGAATGAAGGAAGCACTGAATGGAACGATATGTATAATATGCTGAATGATCGATGTGATAGGTACGGGGAGTGTGGCGCAAATGGCATTTGCAGAATTAACAAGTCACCCATTTGTGACTGCTTGGAGGGGTTTATTCCGAAATCCCAGCAAGAGTGGGTGGTGCTTAATACGACAAGTGGGTGCACAAGGAAGATACAATTGGATTGTCAGAAAGGAGAAGGGTTTCGGGAAATTCAAAATGTAAAACTGCCAGACCTGTTGGATTTTTGGGTCAACAAGAGCCGGAACAAGAAGGAATGCAGGGCAGAGTGTTTGAAGAATTGTTCTTGTATTGCTTATTCTTATTCAGATTACAGTCTTGGAGGCACCGGCTGTCTGATGTGGTTTAAAGACCTTATTGATATCCGTGAGTTTGCTGATGAAAGTGAGCAGCAACGTATTTATATTAGGATGCCAGCTTCAGAACTAGGTCATGCCGGTCAGAAGGAGAAAAGAATAGTGCTCATAGCGGTCATCTCAGCAGCTTGTGTTCTAATTACAGGTGTGTCATGTTGGCTTATAATTTGGAAGAAGAAAGGAAGCGAGAGGGGTTCAAGATCGAATAAGGAAGACATGGAGTTGCCACTGTTTGATTTTCATACAATTGCAGCAGGCACCAACAACTTCTCAGGGACAAACATACTTGGGGAGGGAGGTTTTGGTCCAGTTTACAAGGCCAACCTAGCACAAGAACAACTAGTAGCAGTGAAAAGACTTTCAAAAGATTCCGGGCAAGGTATTGATGAGTTTAAGAATGAAGTTACGATGATAGCCAACCTTCAACACAGGAATCTTGTTAAACTTTTGGGCTGCTGCATTGAAGGACAAGAGAGGATGCTAATCTATGAGTATATGCCTAACAAAAGCTTGGACTGCTTTATTTTCGATGAAAATAGAAAGAAATCGCTCGATTGGCAAAAACGAGTCAACATTATAATTGGAACTGCACGAGGACTTCTCTACCTCCACCAAGACTCCAGATTGAGAATCATTCATAGGGACCTCAAGAGTAGCAACATTTTACTCGACAATGAGCTGAACCCCAAAATATCTGACTTCGGCATAGCAAGAATTTTTGGACTCGATCAGACCGAAGCGAAGACAAAACGAGTGATTGGCACATATGGGTATATGTCTCCCGAGTACGTGATTGATGGCCACTTTTCAGTGAAATCCGATGTCTTTAGTTTTGGAGTGCTTTTGCTAGAGATAGTATGTGGCAGAAAGAACAGAGGGTTTCATCATCCAGATCACCATCACAGTCTTCTGGGACATGCCTGGCTACTATGGAACAAAGAAAAGGTTTTGGAACTATTAGATCCATGCTTGGAGGACTCGTATGTCGAGTTTGAGGTACTAAGATGTATACAAGTGGGTCTATTATGCGTGCAAAAGCGGCCCATCGACAGACCAGTAATGTCATCAGTGGTGTTCATGTTAAGCAATGAGGGAGCAATGTTGCCTCAACCGAAGGAGCCTGGTTTCTTCACAGAACGAAGTTCCATGGATGGTGATACCTTCATAAGTGAAGGAAGAAGCAAAACAGGAAACATGCTTACCATTACCACGATGGAAGCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

841

Amino Acids

94.88

Weight (kDa)

6.19

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 107 - 211 1.1e-33 D-mannose binding lectin
S_locus_glycop PF00954 243 - 349 5.7e-26 S-locus glycoprotein domain
PAN_2 PF08276 370 - 436 2.9e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 527 - 723 2.1e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 527 - 723 5.9e-45 Protein kinase domain
DUF3403 PF11883 797 - 841 8.1e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 791, 1463, 1877
AasI GACNNNNNNGTC 1 cut(s) 2282
Acc36I ACCTGC 1 cut(s) 506
AccB1I GGYRCC 2 cut(s) 1268, 1556
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 2 cut(s) 662, 2308
AccIII TCCGGA 1 cut(s) 263
AciI CCGC 3 cut(s) 892, 1412, 2338
AclWI GGATC 4 cut(s) 452, 638, 721, 2255
AcoI YGGCCR 1 cut(s) 2098
AcsI RAATTY 5 cut(s) 99, 291, 487, 1134, 2013
AcuI CTGAAG 2 cut(s) 894, 1347
AfaI GTAC 5 cut(s) 299, 450, 965, 2087, 2298
AfiI CCNNNNNNNGG 3 cut(s) 353, 653, 746
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 9 cut(s) 245, 312, 455, 1139, 1219, 1497, 1662, 1724, 1763
AhlI ACTAGT 1 cut(s) 1639
AjnI CCWGG 4 cut(s) 352, 740, 2219, 2419
AjuI GAANNNNNNNTTGG 4 cut(s) 340, 372, 1085, 1117
AluBI AGCT 9 cut(s) 210, 472, 697, 868, 1361, 1427, 1809, 1978, 2519
AluI AGCT 9 cut(s) 210, 472, 697, 868, 1361, 1427, 1809, 1978, 2519
Alw21I GWGCWC 2 cut(s) 1088, 1407
Alw26I GTCTC 3 cut(s) 473, 495, 2082
Alw44I GTGCAC 1 cut(s) 1084
AlwI GGATC 4 cut(s) 452, 638, 721, 2255
Ama87I CYCGRG 1 cut(s) 2081
Aor13HI TCCGGA 1 cut(s) 263
AoxI GGCC 5 cut(s) 112, 737, 1617, 2098, 2339
ApaI GGGCCC 1 cut(s) 741
ApaLI GTGCAC 1 cut(s) 1084
ApeKI GCWGC 7 cut(s) 865, 889, 1333, 1424, 1550, 1752, 1755
ApoI RAATTY 5 cut(s) 99, 291, 487, 1134, 2013
Asp700I GAANNNNTTC 1 cut(s) 1125
AspLEI GCGC 1 cut(s) 980
AspS9I GGNCC 7 cut(s) 112, 397, 737, 738, 1604, 1942, 2340
AsuC2I CCSGG 2 cut(s) 604, 1672
AsuHPI GGTGA 6 cut(s) 196, 668, 893, 999, 2186, 2465
AsuII TTCGAA 2 cut(s) 730, 776
AvaI CYCGRG 1 cut(s) 2081
AvaII GGWCC 3 cut(s) 397, 1604, 1942
BaeGI GKGCMC 2 cut(s) 741, 1088
BalI TGGCCA 1 cut(s) 2100
BanI GGYRCC 2 cut(s) 1268, 1556
BanII GRGCYC 3 cut(s) 370, 609, 741
BauI CACGAG 1 cut(s) 1890
BbsI GAAGAC 3 cut(s) 1518, 2048, 2198
Bbv12I GWGCWC 2 cut(s) 1088, 1407
BbvCI CCTCAGC 1 cut(s) 693
BbvI GCAGC 7 cut(s) 877, 901, 1345, 1436, 1562, 1739, 1742
BciT130I CCWGG 4 cut(s) 354, 742, 2221, 2421
BciVI GTATCC 1 cut(s) 553
BcnI CCSGG 2 cut(s) 604, 1672
BcoDI GTCTC 3 cut(s) 473, 495, 2082
BcuI ACTAGT 1 cut(s) 1639
BfaI CTAG 5 cut(s) 639, 1370, 1625, 1640, 2147
BfmI CTRYAG 2 cut(s) 510, 667
BfuAI ACCTGC 1 cut(s) 506
BfuI GTATCC 1 cut(s) 553
BisI GCNGC 8 cut(s) 866, 890, 1334, 1425, 1551, 1753, 1756, 2339
BlsI GCNGC 8 cut(s) 867, 891, 1335, 1426, 1552, 1754, 1757, 2340
BmcAI AGTACT 1 cut(s) 450
Bme1390I CCNGG 6 cut(s) 354, 604, 742, 1672, 2221, 2421
Bme18I GGWCC 3 cut(s) 397, 1604, 1942
BmeT110I CYCGRG 1 cut(s) 2081
BmgT120I GGNCC 7 cut(s) 112, 397, 737, 738, 1604, 1942, 2340
BmiI GGNNCC 6 cut(s) 367, 739, 1270, 1558, 1943, 2418
BmrFI CCNGG 6 cut(s) 354, 604, 742, 1672, 2221, 2421
BmsI GCATC 3 cut(s) 34, 1344, 1767
BpiI GAAGAC 3 cut(s) 1518, 2048, 2198
BplI GAGNNNNNCTC 2 cut(s) 1884, 1916
BpmI CTGGAG 1 cut(s) 1903
Bpu10I CCTNAGC 3 cut(s) 89, 693, 708
Bpu14I TTCGAA 2 cut(s) 730, 776
BpuEI CTTGAG 1 cut(s) 1931
BpuMI CCSGG 2 cut(s) 604, 1672
Bsa29I ATCGAT 1 cut(s) 952
BsaAI YACGTR 2 cut(s) 834, 2089
BsaBI GATNNNNATC 2 cut(s) 1781, 2196
BsaJI CCNNGG 3 cut(s) 219, 740, 2445
BsaWI WCCGGW 2 cut(s) 230, 263
BsaXI ACNNNNNCTCC 8 cut(s) 172, 202, 710, 740, 1901, 1931, 2264, 2294
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 653, 746
Bse118I RCCGGY 2 cut(s) 1271, 1379
Bse1I ACTGG 5 cut(s) 69, 487, 766, 1607, 2354
Bse3DI GCAATG 2 cut(s) 2392, 2403
Bse8I GATNNNNATC 2 cut(s) 1781, 2196
BseAI TCCGGA 1 cut(s) 263
BseBI CCWGG 4 cut(s) 354, 742, 2221, 2421
BseCI ATCGAT 1 cut(s) 952
BseDI CCNNGG 3 cut(s) 219, 740, 2445
BseGI GGATG 6 cut(s) 582, 593, 1359, 1782, 2185, 2455
BseJI GATNNNNATC 2 cut(s) 1781, 2196
BseLI CCNNNNNNNGG 3 cut(s) 353, 653, 746
BseMI GCAATG 2 cut(s) 2392, 2403
BseMII CTCAG 3 cut(s) 707, 1434, 1584
BseNI ACTGG 5 cut(s) 69, 487, 766, 1607, 2354
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 2 cut(s) 741, 1088
BseXI GCAGC 7 cut(s) 877, 901, 1345, 1436, 1562, 1739, 1742
BseYI CCCAGC 1 cut(s) 1047
BsgI GTGCAG 2 cut(s) 192, 1872
BshFI GGCC 5 cut(s) 114, 739, 1619, 2100, 2341
BshNI GGYRCC 2 cut(s) 1268, 1556
BshVI ATCGAT 1 cut(s) 952
BsiHKAI GWGCWC 2 cut(s) 1088, 1407
BsiHKCI CYCGRG 1 cut(s) 2081
BsiSI CCGG 7 cut(s) 231, 264, 603, 1186, 1272, 1380, 1671
BslFI GGGAC 3 cut(s) 1588, 1955, 2226
BslI CCNNNNNNNGG 3 cut(s) 353, 653, 746
BsmAI GTCTC 3 cut(s) 473, 495, 2082
BsmFI GGGAC 3 cut(s) 1588, 1955, 2226
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 5 cut(s) 114, 739, 1619, 2100, 2341
BsoBI CYCGRG 1 cut(s) 2081
Bsp119I TTCGAA 2 cut(s) 730, 776
Bsp120I GGGCCC 1 cut(s) 737
Bsp1286I GDGCHC 5 cut(s) 370, 609, 741, 1088, 1407
Bsp13I TCCGGA 1 cut(s) 263
Bsp143I GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
Bsp19I CCATGG 1 cut(s) 2445
BspACI CCGC 3 cut(s) 892, 1412, 2338
BspANI GGCC 5 cut(s) 114, 739, 1619, 2100, 2341
BspCNI CTCAG 3 cut(s) 706, 1433, 1583
BspDI ATCGAT 1 cut(s) 952
BspEI TCCGGA 1 cut(s) 263
BspLI GGNNCC 6 cut(s) 367, 739, 1270, 1558, 1943, 2418
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 4 cut(s) 452, 638, 721, 2255
BspT104I TTCGAA 2 cut(s) 730, 776
BspT107I GGYRCC 2 cut(s) 1268, 1556
BsrDI GCAATG 2 cut(s) 2392, 2403
BsrFI RCCGGY 2 cut(s) 1271, 1379
BsrI ACTGG 5 cut(s) 69, 487, 766, 1607, 2354
BssAI RCCGGY 2 cut(s) 1271, 1379
BssECI CCNNGG 3 cut(s) 219, 740, 2445
BssMI GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
BssNAI GTATAC 2 cut(s) 663, 2309
BssSI CACGAG 1 cut(s) 1890
BssT1I CCWWGG 2 cut(s) 219, 2445
Bst1107I GTATAC 2 cut(s) 663, 2309
Bst2BI CACGAG 1 cut(s) 1890
Bst2UI CCWGG 4 cut(s) 354, 742, 2221, 2421
Bst4CI ACNGT 6 cut(s) 322, 424, 448, 1259, 1530, 2204
Bst6I CTCTTC 1 cut(s) 260
BstBAI YACGTR 2 cut(s) 834, 2089
BstBI TTCGAA 2 cut(s) 730, 776
BstC8I GCNNGC 3 cut(s) 1359, 1555, 2330
BstDEI CTNAG 6 cut(s) 89, 693, 708, 1420, 1570, 2300
BstDSI CCRYGG 1 cut(s) 2445
BstF5I GGATG 6 cut(s) 582, 593, 1359, 1782, 2185, 2455
BstHHI GCGC 1 cut(s) 980
BstKTI GATC 8 cut(s) 460, 646, 716, 952, 1502, 2029, 2194, 2263
BstMAI GTCTC 3 cut(s) 473, 495, 2082
BstMBI GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
BstMWI GCNNNNNNNGC 6 cut(s) 898, 984, 1625, 1815, 1855, 2338
BstNI CCWGG 4 cut(s) 354, 742, 2221, 2421
BstNSI RCATGY 3 cut(s) 889, 2219, 2497
BstSCI CCNGG 6 cut(s) 352, 602, 740, 1670, 2219, 2419
BstSFI CTRYAG 2 cut(s) 510, 667
BstSLI GKGCMC 2 cut(s) 741, 1088
BstV1I GCAGC 7 cut(s) 877, 901, 1345, 1436, 1562, 1739, 1742
BstV2I GAAGAC 3 cut(s) 1518, 2048, 2198
BstX2I RGATCY 2 cut(s) 457, 2260
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 2 cut(s) 457, 2260
BstZ17I GTATAC 2 cut(s) 663, 2309
Bsu15I ATCGAT 1 cut(s) 952
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 5 cut(s) 114, 739, 1619, 2100, 2341
BsuTUI ATCGAT 1 cut(s) 952
BtgI CCRYGG 1 cut(s) 2445
BtsCI GGATG 6 cut(s) 582, 593, 1359, 1782, 2185, 2455
BtsI GCAGTG 2 cut(s) 175, 1653
BtsIMutI CAGTG 7 cut(s) 175, 420, 915, 1526, 1653, 2115, 2373
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 1359, 1555, 2330
CfoI GCGC 1 cut(s) 980
Cfr10I RCCGGY 2 cut(s) 1271, 1379
Cfr13I GGNCC 7 cut(s) 112, 397, 737, 738, 1604, 1942, 2340
ClaI ATCGAT 1 cut(s) 952
Csp6I GTAC 5 cut(s) 298, 449, 964, 2086, 2297
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviQI GTAC 5 cut(s) 298, 449, 964, 2086, 2297
DdeI CTNAG 6 cut(s) 89, 693, 708, 1420, 1570, 2300
DpnI GATC 8 cut(s) 459, 645, 715, 951, 1501, 2028, 2193, 2262
DpnII GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
DraI TTTAAA 1 cut(s) 1291
DrdI GACNNNNNNGTC 1 cut(s) 2282
DseDI GACNNNNNNGTC 1 cut(s) 2282
EaeI YGGCCR 1 cut(s) 2098
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 219, 2445
Eco24I GRGCYC 3 cut(s) 370, 609, 741
Eco32I GATATC 1 cut(s) 1306
Eco47I GGWCC 3 cut(s) 397, 1604, 1942
Eco57I CTGAAG 2 cut(s) 894, 1347
Eco88I CYCGRG 1 cut(s) 2081
EcoO109I RGGNCCY 3 cut(s) 112, 738, 1942
EcoRII CCWGG 4 cut(s) 352, 740, 2219, 2419
EcoRV GATATC 1 cut(s) 1306
EcoT14I CCWWGG 2 cut(s) 219, 2445
EcoT38I GRGCYC 3 cut(s) 370, 609, 741
ErhI CCWWGG 2 cut(s) 219, 2445
FaqI GGGAC 3 cut(s) 1588, 1955, 2226
FauNDI CATATG 1 cut(s) 2065
FblI GTMKAC 2 cut(s) 662, 2308
Fnu4HI GCNGC 8 cut(s) 866, 890, 1334, 1425, 1551, 1753, 1756, 2339
FokI GGATG 6 cut(s) 589, 600, 1366, 1789, 2172, 2462
FriOI GRGCYC 3 cut(s) 370, 609, 741
Fsp4HI GCNGC 8 cut(s) 866, 890, 1334, 1425, 1551, 1753, 1756, 2339
FspBI CTAG 5 cut(s) 639, 1370, 1625, 1640, 2147
GlaI GCGC 1 cut(s) 979
GluI GCNGC 8 cut(s) 866, 890, 1334, 1425, 1551, 1753, 1756, 2339
GsaI CCCAGC 1 cut(s) 1051
GsuI CTGGAG 1 cut(s) 1903
HaeIII GGCC 5 cut(s) 114, 739, 1619, 2100, 2341
HapII CCGG 7 cut(s) 231, 264, 603, 1186, 1272, 1380, 1671
HhaI GCGC 1 cut(s) 980
Hin6I GCGC 1 cut(s) 978
HinP1I GCGC 1 cut(s) 978
HincII GTYRAC 2 cut(s) 1177, 1870
HindII GTYRAC 2 cut(s) 1177, 1870
HindIII AAGCTT 1 cut(s) 1807
HinfI GANTC 9 cut(s) 252, 548, 1667, 1732, 1866, 1916, 1929, 2022, 2276
HpaII CCGG 7 cut(s) 231, 264, 603, 1186, 1272, 1380, 1671
HphI GGTGA 6 cut(s) 196, 668, 893, 999, 2186, 2465
Hpy166II GTNNAC 8 cut(s) 300, 663, 759, 1086, 1177, 1612, 1870, 2309
Hpy188III TCNNGA 8 cut(s) 264, 619, 1130, 1497, 1920, 1948, 2081, 2189
Hpy8I GTNNAC 8 cut(s) 300, 663, 759, 1086, 1177, 1612, 1870, 2309
HpyCH4III ACNGT 6 cut(s) 322, 424, 448, 1259, 1530, 2204
HpyCH4IV ACGT 3 cut(s) 833, 1339, 2088
HpyF10VI GCNNNNNNNGC 6 cut(s) 898, 984, 1625, 1815, 1855, 2338
HpyF3I CTNAG 6 cut(s) 89, 693, 708, 1420, 1570, 2300
HpySE526I ACGT 3 cut(s) 833, 1339, 2088
HspAI GCGC 1 cut(s) 978
Kpn2I TCCGGA 1 cut(s) 263
Kzo9I GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
LmnI GCTCC 4 cut(s) 215, 365, 2393, 2416
Lsp1109I GCAGC 7 cut(s) 877, 901, 1345, 1436, 1562, 1739, 1742
LweI GCATC 3 cut(s) 34, 1344, 1767
MaeI CTAG 5 cut(s) 639, 1370, 1625, 1640, 2147
MaeII ACGT 3 cut(s) 833, 1339, 2088
MaeIII GTNAC 4 cut(s) 881, 1005, 1016, 1702
MalI GATC 8 cut(s) 459, 645, 715, 951, 1501, 2028, 2193, 2262
MboI GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
MfeI CAATTG 2 cut(s) 1100, 1545
MflI RGATCY 2 cut(s) 457, 2260
MhlI GDGCHC 5 cut(s) 370, 609, 741, 1088, 1407
MlsI TGGCCA 1 cut(s) 2100
MluNI TGGCCA 1 cut(s) 2100
MlyI GAGTC 5 cut(s) 246, 1875, 1910, 2016, 2270
MmeI TCCRAC 2 cut(s) 215, 1143
Mox20I TGGCCA 1 cut(s) 2100
MroI TCCGGA 1 cut(s) 263
MroXI GAANNNNTTC 1 cut(s) 1125
MscI TGGCCA 1 cut(s) 2100
MseI TTAA 8 cut(s) 377, 678, 999, 1068, 1290, 1692, 1740, 2381
Msp20I TGGCCA 1 cut(s) 2100
MspA1I CMGCKG 2 cut(s) 868, 892
MspI CCGG 7 cut(s) 231, 264, 603, 1186, 1272, 1380, 1671
MspR9I CCNGG 6 cut(s) 354, 604, 742, 1672, 2221, 2421
MunI CAATTG 2 cut(s) 1100, 1545
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 4 cut(s) 354, 742, 2221, 2421
MwoI GCNNNNNNNGC 6 cut(s) 898, 984, 1625, 1815, 1855, 2338
NciI CCSGG 2 cut(s) 604, 1672
NcoI CCATGG 1 cut(s) 2445
NdeI CATATG 1 cut(s) 2065
NdeII GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
NlaIV GGNNCC 6 cut(s) 367, 739, 1270, 1558, 1943, 2418
NmuCI GTSAC 3 cut(s) 881, 1005, 1016
NspI RCATGY 3 cut(s) 889, 2219, 2497
NspV TTCGAA 2 cut(s) 730, 776
PcsI WCGNNNNNNNCGW 1 cut(s) 2031
PctI GAATGC 1 cut(s) 1205
PdmI GAANNNNTTC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 548, 1667, 1732, 1929
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 8 cut(s) 867, 891, 1335, 1426, 1552, 1754, 1757, 2340
PleI GAGTC 5 cut(s) 246, 1874, 1910, 2016, 2270
PpsI GAGTC 5 cut(s) 246, 1874, 1910, 2016, 2270
Ppu21I YACGTR 2 cut(s) 834, 2089
PpuMI RGGWCCY 1 cut(s) 1942
PsiI TTATAA 3 cut(s) 791, 1463, 1877
Psp5II RGGWCCY 1 cut(s) 1942
Psp6I CCWGG 4 cut(s) 352, 740, 2219, 2419
PspFI CCCAGC 1 cut(s) 1047
PspGI CCWGG 4 cut(s) 352, 740, 2219, 2419
PspN4I GGNNCC 6 cut(s) 367, 739, 1270, 1558, 1943, 2418
PspOMI GGGCCC 1 cut(s) 737
PspPI GGNCC 7 cut(s) 112, 397, 737, 738, 1604, 1942, 2340
PspPPI RGGWCCY 1 cut(s) 1942
PsuI RGATCY 2 cut(s) 457, 2260
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 5 cut(s) 299, 450, 965, 2087, 2298
RsaNI GTAC 5 cut(s) 298, 449, 964, 2086, 2297
SaqAI TTAA 8 cut(s) 377, 678, 999, 1068, 1290, 1692, 1740, 2381
SatI GCNGC 8 cut(s) 866, 890, 1334, 1425, 1551, 1753, 1756, 2339
Sau3AI GATC 8 cut(s) 457, 643, 713, 949, 1499, 2026, 2191, 2260
Sau96I GGNCC 7 cut(s) 112, 397, 737, 738, 1604, 1942, 2340
ScaI AGTACT 1 cut(s) 450
SchI GAGTC 5 cut(s) 246, 1875, 1910, 2016, 2270
ScrFI CCNGG 6 cut(s) 354, 604, 742, 1672, 2221, 2421
SduI GDGCHC 5 cut(s) 370, 609, 741, 1088, 1407
SfaNI GCATC 3 cut(s) 34, 1344, 1767
SfcI CTRYAG 2 cut(s) 510, 667
SfuI TTCGAA 2 cut(s) 730, 776
SinI GGWCC 3 cut(s) 397, 1604, 1942
SmlI CTYRAG 1 cut(s) 1946
SmoI CTYRAG 1 cut(s) 1946
SpeI ACTAGT 1 cut(s) 1639
SsiI CCGC 3 cut(s) 892, 1412, 2338
SspMI CTAG 5 cut(s) 639, 1370, 1625, 1640, 2147
StyD4I CCNGG 6 cut(s) 352, 602, 740, 1670, 2219, 2419
StyI CCWWGG 2 cut(s) 219, 2445
TaaI ACNGT 6 cut(s) 322, 424, 448, 1259, 1530, 2204
TaiI ACGT 3 cut(s) 836, 1342, 2091
TaqII GACCGA 1 cut(s) 2048
TatI WGTACW 1 cut(s) 448
TauI GCSGC 1 cut(s) 2341
TfiI GAWTC 4 cut(s) 548, 1667, 1732, 1929
Tru1I TTAA 8 cut(s) 377, 678, 999, 1068, 1290, 1692, 1740, 2381
Tru9I TTAA 8 cut(s) 377, 678, 999, 1068, 1290, 1692, 1740, 2381
TscAI CASTG 7 cut(s) 175, 427, 922, 1533, 1653, 2115, 2373
TseFI GTSAC 3 cut(s) 881, 1005, 1016
TseI GCWGC 7 cut(s) 865, 889, 1333, 1424, 1550, 1752, 1755
Tsp45I GTSAC 3 cut(s) 881, 1005, 1016
TspGWI ACGGA 1 cut(s) 1298
TspRI CASTG 7 cut(s) 175, 427, 922, 1533, 1653, 2115, 2373
Van91I CCANNNNNTGG 1 cut(s) 653
VneI GTGCAC 1 cut(s) 1084
VpaK11BI GGWCC 3 cut(s) 397, 1604, 1942
XapI RAATTY 5 cut(s) 99, 291, 487, 1134, 2013
XceI RCATGY 3 cut(s) 889, 2219, 2497
XcmI CCANNNNNNNNNTGG 1 cut(s) 2509
XmiI GTMKAC 2 cut(s) 662, 2308
XmnI GAANNNNTTC 1 cut(s) 1125
XspI CTAG 5 cut(s) 639, 1370, 1625, 1640, 2147
ZrmI AGTACT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.