Rw5G022860

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001151
Physical Location & Seq
Forward (+)
31858874 .. 31860866
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022860.1

Sequence Viewer

Length: 717 bp
ATGATCAGTGCAATAATTATGGGGAGTGTGGAGCAAATGGCATTTGCAGGATTAACAAGGTACCAATTTGCGATTGCTTGCAGGGGTTTGTTCCTAAATCACCGAAAGAGTGGGTGGTGCTTAATAGGACAGGTGGTTGACGGCACTAGATTTCCAGAAGGGAGAAGGGTTTCTGAACTGCAAAATGTGAAACCGCCAGACCTGCTGGATATTTTGGTGAACAAGAGCATGAGCATGGAGGAATGTGAGGCAAATTGCTTGAAGAATTGTTCGTGCATAGCTTATTCTAATTCAGATATTTGGAGTGGAGGCAGTGGCTGTGTTATGTGGTTTGGAAATCTGATTGATATGCGGGAGTTTGTTGAGGATCTTGGTGAGCAAGATATCTTTATTCGGATGCCAGCTTCTGAACTAGGTGCTACCAGACTTGCCAGCAAGAAGCATAACAGAGTAGTACTCATCTCTGTCCTATCAGCAGCTTCTGTGCTTCTTTTTCTGGGTTTGTTATGTTGGTGTGTAATTCTGAAGCAGAGACGAAACAAACGGGGTTCAAGAGCTAGTAAGGAGGACTTGGAGTTGCCCCAGTTTGATTTTCATACTATTGCAACTGCCACTGATAAATTCTCCAGCAGAAATAAACTTGGAGAGGGAGGTTTCGGTCCAGTTTACAAGGCCAAACTAACACAAGAAGAATCAATAGCAGTAAAGAGACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

238

Amino Acids

26.49

Weight (kDa)

8.73

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 58 - 117 7.5e-19 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 60
AciI CCGC 2 cut(s) 194, 352
AclWI GGATC 1 cut(s) 375
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 2 cut(s) 62, 456
AgsI TTSAA 2 cut(s) 262, 552
AluBI AGCT 4 cut(s) 281, 404, 479, 557
AluI AGCT 4 cut(s) 281, 404, 479, 557
Alw26I GTCTC 2 cut(s) 526, 703
AlwI GGATC 1 cut(s) 375
AlwNI CAGNNNCTG 3 cut(s) 318, 407, 482
AoxI GGCC 1 cut(s) 672
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 1 cut(s) 620
Asp700I GAANNNNTTC 1 cut(s) 169
Asp718I GGTACC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 659
AsuHPI GGTGA 3 cut(s) 92, 229, 386
AvaII GGWCC 1 cut(s) 659
BanI GGYRCC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 488
BceAI ACGGC 1 cut(s) 157
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 526, 703
BfaI CTAG 3 cut(s) 147, 413, 558
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 1 cut(s) 477
BlsI GCNGC 1 cut(s) 478
BmcAI AGTACT 1 cut(s) 456
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 1 cut(s) 62
BmrI ACTGGG 1 cut(s) 577
BmsI GCATC 1 cut(s) 387
BmuI ACTGGG 1 cut(s) 577
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpmI CTGGAG 1 cut(s) 610
Bse1I ACTGG 2 cut(s) 583, 662
BseGI GGATG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 583, 662
BseXI GCAGC 1 cut(s) 488
BshFI GGCC 1 cut(s) 674
BshNI GGYRCC 1 cut(s) 60
BsmAI GTCTC 2 cut(s) 526, 703
BsmBI CGTCTC 1 cut(s) 526
BsnI GGCC 1 cut(s) 674
Bsp143I GATC 2 cut(s) 3, 367
BspACI CCGC 2 cut(s) 194, 352
BspANI GGCC 1 cut(s) 674
BspLI GGNNCC 1 cut(s) 62
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 60
BsrI ACTGG 2 cut(s) 583, 662
BssMI GATC 2 cut(s) 3, 367
BstC8I GCNNGC 3 cut(s) 79, 402, 433
BstF5I GGATG 1 cut(s) 402
BstKTI GATC 2 cut(s) 6, 370
BstMAI GTCTC 2 cut(s) 526, 703
BstMBI GATC 2 cut(s) 3, 367
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 1 cut(s) 674
BtsCI GGATG 1 cut(s) 402
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 13, 319, 612
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 79, 402, 433
CaiI CAGNNNCTG 3 cut(s) 318, 407, 482
Cfr13I GGNCC 1 cut(s) 659
Csp6I GTAC 2 cut(s) 61, 455
CviAII CATG 2 cut(s) 229, 235
CviJI RGCY 6 cut(s) 281, 318, 404, 479, 557, 674
CviKI_1 RGCY 6 cut(s) 281, 318, 404, 479, 557, 674
CviQI GTAC 2 cut(s) 61, 455
DpnI GATC 2 cut(s) 5, 369
DpnII GATC 2 cut(s) 3, 367
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 659
Eco57I CTGAAG 1 cut(s) 545
EcoRV GATATC 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 2 cut(s) 232, 238
FaiI YATR 9 cut(s) 20, 230, 236, 278, 326, 350, 444, 508, 597
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FatI CATG 2 cut(s) 228, 234
FauI CCCGC 1 cut(s) 345
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 477
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 477
FspBI CTAG 3 cut(s) 147, 413, 558
GluI GCNGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 610
HaeIII GGCC 1 cut(s) 674
Hin1II CATG 2 cut(s) 232, 238
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 2 cut(s) 692, 711
HphI GGTGA 3 cut(s) 92, 229, 386
Hpy166II GTNNAC 3 cut(s) 139, 220, 667
Hpy188I TCNGA 6 cut(s) 175, 295, 342, 396, 409, 525
Hpy188III TCNNGA 2 cut(s) 155, 552
Hpy8I GTNNAC 3 cut(s) 139, 220, 667
HpyAV CCTTC 2 cut(s) 152, 159
HpyCH4V TGCA 6 cut(s) 11, 47, 81, 181, 276, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
Hsp92II CATG 2 cut(s) 232, 238
KpnI GGTACC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 367
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 1 cut(s) 387
MaeI CTAG 3 cut(s) 147, 413, 558
MalI GATC 2 cut(s) 5, 369
MboI GATC 2 cut(s) 3, 367
MboII GAAGA 2 cut(s) 274, 701
MflI RGATCY 1 cut(s) 367
MluCI AATT 7 cut(s) 15, 65, 253, 265, 289, 519, 620
MlyI GAGTC 1 cut(s) 705
MnlI CCTC 7 cut(s) 232, 241, 302, 358, 559, 640, 644
MroXI GAANNNNTTC 1 cut(s) 169
MseI TTAA 2 cut(s) 53, 122
MslI CAYNNNNRTG 1 cut(s) 233
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeII GATC 2 cut(s) 3, 367
NlaIII CATG 2 cut(s) 232, 238
NlaIV GGNNCC 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 692
PkrI GCNGC 1 cut(s) 478
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 659
PstNI CAGNNNCTG 3 cut(s) 318, 407, 482
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 62, 456
RsaNI GTAC 2 cut(s) 61, 455
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 2 cut(s) 53, 122
SatI GCNGC 1 cut(s) 477
Sau3AI GATC 2 cut(s) 3, 367
Sau96I GGNCC 1 cut(s) 659
ScaI AGTACT 1 cut(s) 456
SchI GAGTC 1 cut(s) 705
SetI ASST 9 cut(s) 62, 135, 204, 283, 406, 418, 481, 559, 655
SfaNI GCATC 1 cut(s) 387
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 7 cut(s) 15, 65, 253, 265, 289, 519, 620
SsiI CCGC 2 cut(s) 194, 352
SspMI CTAG 3 cut(s) 147, 413, 558
TaqII GACCGA 1 cut(s) 647
TasI AATT 7 cut(s) 15, 65, 253, 265, 289, 519, 620
TatI WGTACW 1 cut(s) 454
TfiI GAWTC 1 cut(s) 692
Tru1I TTAA 2 cut(s) 53, 122
Tru9I TTAA 2 cut(s) 53, 122
TscAI CASTG 3 cut(s) 13, 319, 619
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 1 cut(s) 584
TspRI CASTG 3 cut(s) 13, 319, 619
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 169
XspI CTAG 3 cut(s) 147, 413, 558
ZrmI AGTACT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.