Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Reverse (-)

33753286 .. 33755572

Gene Structure 2287 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g39831.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGCTCTTCGGCGTCTCTTTCCACTATTGGCAATTCGGCGAGAAAGCGTTTCATGTGAAGAGTCAAGAAGTTATTGGTCTTCTGGGTGAGCTTGGTTACAGCCTCTTCCTGTTTGTACTAGGAACGACAGTGGATTTGGGAATCATAAAAAGATCAGGATATAAAGCCATCCTCACCGGGGTTTTAGGCATGTTGGTCCCTTTGGCAAGCGGTGAGGTTGTCCAAAGGCTTATTGTGAAATATGTCTATAACCTTGACAAGAGAGACACCACTCGGCTTGAGGTTGTGACCGCGACGCATAGCATAACTGCGTTTCCGGTTGTTCTTCTTCTTCTCGAGGACCTGCAGCTCCTCACATCTGAAATTGGCCGGTTAAGCCAGTCTGCAACATTAGTCTGTGACGTCTTTACCGTGTGTCTCCAATATTTTATAGGCAGCTCATCATTTTCGAAGAGTAGTAAACTTGACACTAACAGTATTAACCTCGGAGCAGCCATTGCTTATGTTATTTCTGTTGCTGTGATATTTAGACCAATAATGTTATGGATGGTCAGGCAGACCCCAGAAAACAGGCCTGTCAAGAAAAGCTATCTCAGTGCCCTAATTCTGGTGGTGCTTGGTTCTGGGATACTTACACATTATTACGGCCAGTCTTTTCATTTTGGACCTTTTGTATTTGGTCTGGCTGTCCCAGCTGGACGGCCTCTAGGATCTGCTGTTCAAGAAAACTTCCAACTCTTCATTTCAGAAGTGTTTCTGCCTATTTATGTGACGATAAATGCAATGAGGCCGGACTTTTGGTTAATCGACTACAAAGACGATGCAACAAAAACCACTGCTTTCCTCATGGTCTTCGTTTTTGGGGTCAAAATCTTAGCCACCATGGGTACTCAATTGTATTTCCGGATGCCCTTGAAGGATGCATTTGTTCTTGCTCTCATTTTAAGCTGCAAAGGAATTGTCCACCTTTCAGCCTATACTAGTTTCAGAGATTTCCGGATAGTAAATGATTCGGTGTATGCTGTGATGTTTTCTAGCGTTGTGGTGACTTCAGTACTCGTGCCTATTGTAGTGAAGCACCTCTACGAACCTTCGAGAAAATACGCAGGCTACGAGAGAAGGGACATTATGCATTTGAAACCCAATGCAGAGCTCAAAGTTCTTACCTGCATCCACAGATCATCTAACATGCCTGCAGTGATCAATCTACTCGATGCAGCATGCCCATCTAAAGAAAACCCCATCACTGTTTATGTTCTCCATCTCATCGAATTAATCGGTAGAGTGACTCCCATTTTTATCTCCCACCAAGCGCAGGATAAAACAGATTCCCATATTAGCACATCTTCAGCAAATGTCATCCTTTATTTCAATCACTTCGCGAGCGAAAATCGTGGTGCTGTTAATTTAAGCGTATTCACGGCAATCTCTCCACCGAAATTAATGCAAGAAGATATCTGCACGCTTGCAATGGACAAGCTCACAACCCTAATAGTCCTCCCTTTTCATCGGGAATGGTCAATCGATGGTTCTAGTATTGAGTCGGAAGACAATACTATAAGGTCACTGAACTTCAGTGTACTCGAAAGAGCTCCTTGCTCCGTCGGAATCCTAGTGGATCGGGGACATTTAGGCCGGTCTACCTCCGTTGTATCCGCATTATCAACATTTTCGGTTGCGGTAGTCTTCTTGGGAGGGAAAGATGACAGGGAGGCATTGGCCTTTGCCAAAAGAATGGCCAAGGACTCGTCGATTAGGTTAACAGTAATCCATTTAGTAGCCATGGGTAAGGAATATGATGGTGGGGAATGGGATAAAATACTTGATGCAGAGTCATTGAAGGAATTCAAATATAACAATGTGGGCGGAGGCCTTGTAGTATATGTAGAAGAGATGGTGAAGGATGGACCGGAAACGGCCTTTCTCTTAAGGTCTATGGTGGATGAGTATGATATAATCATAGTTGGAAGAAGGCATGAGGTAGAGTCACCTCAGACAGTAGGGCTTTCGCAATGGAGTGAATTCCCGGAGTTGGGGACGATAGGAGATTTGCTTGCATCACCTGATCTCGATTGTAAGGCATCCATTCTGGTGATACAACAACAACGGTGGATCAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

707

Amino Acids

78.36

Weight (kDa)

6.4

Isoelectric Point (pI)

40.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	1 - 360	3.2e-25	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	416 - 546	6.9e-12	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	557 - 702	2.1e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	405
Acc36I	ACCTGC	2 cut(s)	351, 1175
AccI	GTMKAC	1 cut(s)	1640
AccII	CGCG	2 cut(s)	293, 1382
AccIII	TCCGGA	2 cut(s)	903, 996
AciI	CCGC	5 cut(s)	210, 291, 1656, 1679, 1866
AclWI	GGATC	3 cut(s)	718, 1626, 2120
AcoI	YGGCCR	3 cut(s)	367, 646, 1737
AcsI	RAATTY	2 cut(s)	1844, 2021
AcuI	CTGAAG	3 cut(s)	1035, 1332, 1558
AcyI	GRCGYC	2 cut(s)	12, 402
AfaI	GTAC	4 cut(s)	117, 889, 1056, 1581
AfiI	CCNNNNNNNGG	5 cut(s)	178, 607, 1315, 2032, 2033
AflII	CTTAAG	1 cut(s)	1927
AgsI	TTSAA	6 cut(s)	722, 916, 1138, 1372, 1840, 1849
AhlI	ACTAGT	1 cut(s)	980
AloI	GAACNNNNNNTCC	2 cut(s)	702, 734
AluBI	AGCT	9 cut(s)	91, 349, 438, 588, 695, 948, 1153, 1480, 1592
AluI	AGCT	9 cut(s)	91, 349, 438, 588, 695, 948, 1153, 1480, 1592
Alw21I	GWGCWC	2 cut(s)	1155, 1594
Alw26I	GTCTC	3 cut(s)	19, 258, 422
AlwI	GGATC	3 cut(s)	718, 1626, 2120
Ama87I	CYCGRG	1 cut(s)	335
Aor13HI	TCCGGA	2 cut(s)	903, 996
ApeKI	GCWGC	5 cut(s)	346, 435, 491, 948, 1217
ApoI	RAATTY	2 cut(s)	1844, 2021
ArsI	GACNNNNNNTTYG	4 cut(s)	118, 150, 945, 977
AseI	ATTAAT	2 cut(s)	1274, 1442
Asp700I	GAANNNNTTC	2 cut(s)	753, 1844
AspLEI	GCGC	1 cut(s)	1315
AspS9I	GGNCC	4 cut(s)	196, 340, 665, 1907
AsuC2I	CCSGG	2 cut(s)	178, 2027
AsuHPI	GGTGA	8 cut(s)	98, 166, 224, 1057, 1909, 1980, 2052, 2104
AsuII	TTCGAA	1 cut(s)	449
AvaI	CYCGRG	1 cut(s)	335
AvaII	GGWCC	4 cut(s)	196, 340, 665, 1907
BaeGI	GKGCMC	1 cut(s)	601
BalI	TGGCCA	1 cut(s)	1739
BanII	GRGCYC	2 cut(s)	1155, 1594
BarI	GAAGNNNNNNTAC	2 cut(s)	1067, 1099
BauI	CACGAG	1 cut(s)	1058
BbsI	GAAGAC	4 cut(s)	71, 844, 1554, 1678
Bbv12I	GWGCWC	2 cut(s)	1155, 1594
BbvI	GCAGC	5 cut(s)	358, 447, 503, 935, 1229
BccI	CCATC	9 cut(s)	176, 541, 1234, 1250, 1269, 1520, 1793, 1888, 1898
BceAI	ACGGC	4 cut(s)	661, 716, 1437, 1932
BcgI	CGANNNNNNTGC	2 cut(s)	1426, 1460
BciVI	GTATCC	2 cut(s)	621, 1663
BclI	TGATCA	1 cut(s)	1200
BcnI	CCSGG	2 cut(s)	178, 2027
BcoDI	GTCTC	3 cut(s)	19, 258, 422
BcuI	ACTAGT	1 cut(s)	980
BfaI	CTAG	6 cut(s)	119, 707, 981, 1035, 1533, 1613
BfmI	CTRYAG	2 cut(s)	344, 1194
BfrI	CTTAAG	1 cut(s)	1927
BfuAI	ACCTGC	2 cut(s)	351, 1175
BfuI	GTATCC	2 cut(s)	621, 1663
BisI	GCNGC	5 cut(s)	347, 436, 492, 949, 1218
BlsI	GCNGC	5 cut(s)	348, 437, 493, 950, 1219
BmcAI	AGTACT	1 cut(s)	1056
Bme1390I	CCNGG	2 cut(s)	178, 2027
Bme18I	GGWCC	4 cut(s)	196, 340, 665, 1907
BmeT110I	CYCGRG	1 cut(s)	335
BmgT120I	GGNCC	4 cut(s)	196, 340, 665, 1907
BmiI	GGNNCC	1 cut(s)	198
BmrFI	CCNGG	2 cut(s)	178, 2027
BmsI	GCATC	8 cut(s)	811, 897, 910, 1179, 1204, 1816, 2066, 2090
BpiI	GAAGAC	4 cut(s)	71, 844, 1554, 1678
Bpu14I	TTCGAA	1 cut(s)	449
BpuEI	CTTGAG	1 cut(s)	299
BpuMI	CCSGG	2 cut(s)	178, 2027
Bsa29I	ATCGAT	1 cut(s)	1524
BsaHI	GRCGYC	2 cut(s)	12, 402
BsaJI	CCNNGG	5 cut(s)	177, 484, 882, 1740, 1782
BsaWI	WCCGGW	4 cut(s)	316, 903, 996, 1909
BsaXI	ACNNNNNCTCC	2 cut(s)	1860, 1890
Bsc4I	CCNNNNNNNGG	5 cut(s)	178, 607, 1315, 2032, 2033
Bse118I	RCCGGY	2 cut(s)	369, 1635
Bse1I	ACTGG	2 cut(s)	379, 649
Bse3DI	GCAATG	4 cut(s)	495, 789, 1476, 2018
BseAI	TCCGGA	2 cut(s)	903, 996
BseCI	ATCGAT	1 cut(s)	1524
BseDI	CCNNGG	5 cut(s)	177, 484, 882, 1740, 1782
BseGI	GGATG	9 cut(s)	168, 552, 912, 925, 1170, 1359, 1909, 1948, 2081
BseLI	CCNNNNNNNGG	5 cut(s)	178, 607, 1315, 2032, 2033
BseMI	GCAATG	4 cut(s)	495, 789, 1476, 2018
BseMII	CTCAG	2 cut(s)	607, 2006
BseNI	ACTGG	2 cut(s)	379, 649
BseRI	GAGGAG	1 cut(s)	341
BseSI	GKGCMC	1 cut(s)	601
BseXI	GCAGC	5 cut(s)	358, 447, 503, 935, 1229
BseYI	CCCAGC	1 cut(s)	691
BsgI	GTGCAG	1 cut(s)	1444
Bsh1236I	CGCG	2 cut(s)	293, 1382
BshVI	ATCGAT	1 cut(s)	1524
BsiHKAI	GWGCWC	2 cut(s)	1155, 1594
BsiHKCI	CYCGRG	1 cut(s)	335
BsiSI	CCGG	9 cut(s)	177, 317, 370, 791, 904, 997, 1636, 1910, 2027
BslFI	GGGAC	5 cut(s)	182, 674, 1136, 1638, 2050
BslI	CCNNNNNNNGG	5 cut(s)	178, 607, 1315, 2032, 2033
BsmAI	GTCTC	3 cut(s)	19, 258, 422
BsmBI	CGTCTC	1 cut(s)	19
BsmFI	GGGAC	5 cut(s)	182, 674, 1136, 1638, 2050
BsoBI	CYCGRG	1 cut(s)	335
Bsp119I	TTCGAA	1 cut(s)	449
Bsp1286I	GDGCHC	3 cut(s)	601, 1155, 1594
Bsp13I	TCCGGA	2 cut(s)	903, 996
Bsp143I	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
Bsp19I	CCATGG	2 cut(s)	882, 1782
Bsp68I	TCGCGA	1 cut(s)	1382
BspACI	CCGC	5 cut(s)	210, 291, 1656, 1679, 1866
BspCNI	CTCAG	2 cut(s)	606, 2005
BspDI	ATCGAT	1 cut(s)	1524
BspEI	TCCGGA	2 cut(s)	903, 996
BspFNI	CGCG	2 cut(s)	293, 1382
BspLI	GGNNCC	1 cut(s)	198
BspMAI	CTGCAG	2 cut(s)	348, 1198
BspMI	ACCTGC	2 cut(s)	351, 1175
BspPI	GGATC	3 cut(s)	718, 1626, 2120
BspQI	GCTCTTC	1 cut(s)	11
BspT104I	TTCGAA	1 cut(s)	449
BspTI	CTTAAG	1 cut(s)	1927
BsrDI	GCAATG	4 cut(s)	495, 789, 1476, 2018
BsrFI	RCCGGY	2 cut(s)	369, 1635
BsrI	ACTGG	2 cut(s)	379, 649
BssAI	RCCGGY	2 cut(s)	369, 1635
BssECI	CCNNGG	5 cut(s)	177, 484, 882, 1740, 1782
BssMI	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
BssNI	GRCGYC	2 cut(s)	12, 402
BssSI	CACGAG	1 cut(s)	1058
BssT1I	CCWWGG	3 cut(s)	882, 1740, 1782
Bst2BI	CACGAG	1 cut(s)	1058
Bst4CI	ACNGT	7 cut(s)	130, 412, 476, 1249, 1765, 1999, 2109
Bst6I	CTCTTC	6 cut(s)	11, 53, 110, 446, 743, 1884
BstACI	GRCGYC	2 cut(s)	12, 402
BstAFI	CTTAAG	1 cut(s)	1927
BstAPI	GCANNNNNTGC	1 cut(s)	497
BstBI	TTCGAA	1 cut(s)	449
BstC8I	GCNNGC	8 cut(s)	208, 1108, 1194, 1222, 1384, 1463, 1467, 2055
BstDEI	CTNAG	3 cut(s)	593, 874, 1992
BstDSI	CCRYGG	2 cut(s)	882, 1782
BstF5I	GGATG	9 cut(s)	168, 552, 912, 925, 1170, 1359, 1909, 1948, 2081
BstFNI	CGCG	2 cut(s)	293, 1382
BstHHI	GCGC	1 cut(s)	1315
BstKTI	GATC	7 cut(s)	155, 713, 1181, 1203, 1621, 2068, 2115
BstMAI	GTCTC	3 cut(s)	19, 258, 422
BstMBI	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
BstMWI	GCNNNNNNNGC	3 cut(s)	375, 497, 692
BstNSI	RCATGY	3 cut(s)	193, 1192, 1224
BstSCI	CCNGG	2 cut(s)	176, 2025
BstSFI	CTRYAG	2 cut(s)	344, 1194
BstSLI	GKGCMC	1 cut(s)	601
BstUI	CGCG	2 cut(s)	293, 1382
BstV1I	GCAGC	5 cut(s)	358, 447, 503, 935, 1229
BstV2I	GAAGAC	4 cut(s)	71, 844, 1554, 1678
BstX2I	RGATCY	1 cut(s)	710
BstXI	CCANNNNNNTGG	1 cut(s)	1735
BstYI	RGATCY	1 cut(s)	710
Bsu15I	ATCGAT	1 cut(s)	1524
BsuI	GTATCC	2 cut(s)	621, 1663
BsuTUI	ATCGAT	1 cut(s)	1524
BtgI	CCRYGG	2 cut(s)	882, 1782
BtsCI	GGATG	9 cut(s)	168, 552, 912, 925, 1170, 1359, 1909, 1948, 2081
BtsI	GCAGTG	2 cut(s)	834, 1203
BtsIMutI	CAGTG	7 cut(s)	135, 601, 834, 1203, 1245, 1565, 1582
BtuMI	TCGCGA	1 cut(s)	1382
BveI	ACCTGC	2 cut(s)	351, 1175
Cac8I	GCNNGC	8 cut(s)	208, 1108, 1194, 1222, 1384, 1463, 1467, 2055
CfoI	GCGC	1 cut(s)	1315
Cfr10I	RCCGGY	2 cut(s)	369, 1635
Cfr13I	GGNCC	4 cut(s)	196, 340, 665, 1907
ClaI	ATCGAT	1 cut(s)	1524
CseI	GACGC	1 cut(s)	304
Csp6I	GTAC	4 cut(s)	116, 888, 1055, 1580
CspCI	CAANNNNNGTGG	3 cut(s)	259, 294, 2090
CviAII	CATG	8 cut(s)	53, 190, 847, 883, 1189, 1221, 1783, 1976
CviQI	GTAC	4 cut(s)	116, 888, 1055, 1580
DdeI	CTNAG	3 cut(s)	593, 874, 1992
DpnI	GATC	7 cut(s)	154, 712, 1180, 1202, 1620, 2067, 2114
DpnII	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
EaeI	YGGCCR	3 cut(s)	367, 646, 1737
Eam1104I	CTCTTC	6 cut(s)	11, 53, 110, 446, 743, 1884
EarI	CTCTTC	6 cut(s)	11, 53, 110, 446, 743, 1884
EciI	GGCGGA	1 cut(s)	1881
Ecl136II	GAGCTC	2 cut(s)	1153, 1592
Eco130I	CCWWGG	3 cut(s)	882, 1740, 1782
Eco147I	AGGCCT	2 cut(s)	574, 1872
Eco24I	GRGCYC	2 cut(s)	1155, 1594
Eco32I	GATATC	1 cut(s)	1456
Eco47I	GGWCC	4 cut(s)	196, 340, 665, 1907
Eco53kI	GAGCTC	2 cut(s)	1153, 1592
Eco57I	CTGAAG	3 cut(s)	1035, 1332, 1558
Eco88I	CYCGRG	1 cut(s)	335
EcoICRI	GAGCTC	2 cut(s)	1153, 1592
EcoO109I	RGGNCCY	1 cut(s)	340
EcoRI	GAATTC	2 cut(s)	1844, 2021
EcoRV	GATATC	1 cut(s)	1456
EcoT14I	CCWWGG	3 cut(s)	882, 1740, 1782
EcoT22I	ATGCAT	2 cut(s)	925, 1134
EcoT38I	GRGCYC	2 cut(s)	1155, 1594
ErhI	CCWWGG	3 cut(s)	882, 1740, 1782
Esp3I	CGTCTC	1 cut(s)	19
FaeI	CATG	8 cut(s)	56, 193, 850, 886, 1192, 1224, 1786, 1979
FalI	AAGNNNNNCTT	2 cut(s)	1579, 1611
FaqI	GGGAC	5 cut(s)	182, 674, 1136, 1638, 2050
FatI	CATG	8 cut(s)	52, 189, 846, 882, 1188, 1220, 1782, 1975
FbaI	TGATCA	1 cut(s)	1200
FblI	GTMKAC	1 cut(s)	1640
Fnu4HI	GCNGC	5 cut(s)	347, 436, 492, 949, 1218
FokI	GGATG	9 cut(s)	155, 559, 919, 932, 1157, 1346, 1916, 1955, 2068
FriOI	GRGCYC	2 cut(s)	1155, 1594
Fsp4HI	GCNGC	5 cut(s)	347, 436, 492, 949, 1218
FspBI	CTAG	6 cut(s)	119, 707, 981, 1035, 1533, 1613
GlaI	GCGC	1 cut(s)	1314
GluI	GCNGC	5 cut(s)	347, 436, 492, 949, 1218
GsaI	CCCAGC	1 cut(s)	695
HapII	CCGG	9 cut(s)	177, 317, 370, 791, 904, 997, 1636, 1910, 2027
HgaI	GACGC	1 cut(s)	304
HhaI	GCGC	1 cut(s)	1315
Hin1I	GRCGYC	2 cut(s)	12, 402
Hin1II	CATG	8 cut(s)	56, 193, 850, 886, 1192, 1224, 1786, 1979
Hin6I	GCGC	1 cut(s)	1313
HinP1I	GCGC	1 cut(s)	1313
HincII	GTYRAC	1 cut(s)	1761
HindII	GTYRAC	1 cut(s)	1761
HpaI	GTTAAC	1 cut(s)	1761
HpaII	CCGG	9 cut(s)	177, 317, 370, 791, 904, 997, 1636, 1910, 2027
HphI	GGTGA	8 cut(s)	98, 166, 224, 1057, 1909, 1980, 2052, 2104
Hpy166II	GTNNAC	5 cut(s)	461, 964, 1580, 1641, 1761
Hpy188I	TCNGA	7 cut(s)	361, 488, 748, 989, 1546, 1607, 1995
Hpy8I	GTNNAC	5 cut(s)	461, 964, 1580, 1641, 1761
Hpy99I	CGWCG	3 cut(s)	298, 1607, 1753
HpyAV	CCTTC	6 cut(s)	910, 1101, 1113, 1834, 1894, 1965
HpyCH4III	ACNGT	7 cut(s)	130, 412, 476, 1249, 1765, 1999, 2109
HpyCH4IV	ACGT	1 cut(s)	402
HpyF10VI	GCNNNNNNNGC	3 cut(s)	375, 497, 692
HpyF3I	CTNAG	3 cut(s)	593, 874, 1992
HpySE526I	ACGT	1 cut(s)	402
Hsp92I	GRCGYC	2 cut(s)	12, 402
Hsp92II	CATG	8 cut(s)	56, 193, 850, 886, 1192, 1224, 1786, 1979
HspAI	GCGC	1 cut(s)	1313
Kpn2I	TCCGGA	2 cut(s)	903, 996
Ksp22I	TGATCA	1 cut(s)	1200
KspAI	GTTAAC	1 cut(s)	1761
Kzo9I	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
LguI	GCTCTTC	1 cut(s)	11
LmnI	GCTCC	4 cut(s)	354, 488, 1597, 1604
Lsp1109I	GCAGC	5 cut(s)	358, 447, 503, 935, 1229
LweI	GCATC	8 cut(s)	811, 897, 910, 1179, 1204, 1816, 2066, 2090
MaeI	CTAG	6 cut(s)	119, 707, 981, 1035, 1533, 1613
MaeII	ACGT	1 cut(s)	402
MaeIII	GTNAC	8 cut(s)	95, 286, 398, 769, 1045, 1285, 1563, 1986
MalI	GATC	7 cut(s)	154, 712, 1180, 1202, 1620, 2067, 2114
MboI	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
MfeI	CAATTG	1 cut(s)	893
MflI	RGATCY	1 cut(s)	710
MhlI	GDGCHC	3 cut(s)	601, 1155, 1594
MlsI	TGGCCA	1 cut(s)	1739
MluNI	TGGCCA	1 cut(s)	1739
MlyI	GAGTC	6 cut(s)	70, 1282, 1550, 1739, 1841, 1994
MmeI	TCCRAC	4 cut(s)	757, 1524, 1585, 1945
Mox20I	TGGCCA	1 cut(s)	1739
Mph1103I	ATGCAT	2 cut(s)	925, 1134
MroI	TCCGGA	2 cut(s)	903, 996
MroXI	GAANNNNTTC	2 cut(s)	753, 1844
MscI	TGGCCA	1 cut(s)	1739
MslI	CAYNNNNRTG	1 cut(s)	2090
Msp20I	TGGCCA	1 cut(s)	1739
MspA1I	CMGCKG	1 cut(s)	695
MspCI	CTTAAG	1 cut(s)	1927
MspI	CCGG	9 cut(s)	177, 317, 370, 791, 904, 997, 1636, 1910, 2027
MspR9I	CCNGG	2 cut(s)	178, 2027
MunI	CAATTG	1 cut(s)	893
MvnI	CGCG	2 cut(s)	293, 1382
MwoI	GCNNNNNNNGC	3 cut(s)	375, 497, 692
NciI	CCSGG	2 cut(s)	178, 2027
NcoI	CCATGG	2 cut(s)	882, 1782
NdeII	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
NlaIII	CATG	8 cut(s)	56, 193, 850, 886, 1192, 1224, 1786, 1979
NlaIV	GGNNCC	1 cut(s)	198
NmeAIII	GCCGAG	1 cut(s)	253
NmuCI	GTSAC	7 cut(s)	286, 398, 769, 1045, 1285, 1563, 1986
NruI	TCGCGA	1 cut(s)	1382
NsiI	ATGCAT	2 cut(s)	925, 1134
NspI	RCATGY	3 cut(s)	193, 1192, 1224
NspV	TTCGAA	1 cut(s)	449
PaeI	GCATGC	1 cut(s)	1224
PaeR7I	CTCGAG	1 cut(s)	335
PceI	AGGCCT	2 cut(s)	574, 1872
PciSI	GCTCTTC	1 cut(s)	11
PcsI	WCGNNNNNNNCGW	2 cut(s)	408, 1110
PdmI	GAANNNNTTC	2 cut(s)	753, 1844
PfeI	GAWTC	4 cut(s)	141, 1010, 1328, 1608
PfoI	TCCNGGA	1 cut(s)	2025
PkrI	GCNGC	5 cut(s)	348, 437, 493, 950, 1219
PleI	GAGTC	6 cut(s)	69, 1282, 1549, 1739, 1840, 1993
PpsI	GAGTC	6 cut(s)	69, 1282, 1549, 1739, 1840, 1993
PpuMI	RGGWCCY	1 cut(s)	340
PshBI	ATTAAT	2 cut(s)	1274, 1442
Psp124BI	GAGCTC	2 cut(s)	1155, 1594
Psp5II	RGGWCCY	1 cut(s)	340
PspFI	CCCAGC	1 cut(s)	691
PspN4I	GGNNCC	1 cut(s)	198
PspPI	GGNCC	4 cut(s)	196, 340, 665, 1907
PspPPI	RGGWCCY	1 cut(s)	340
PstI	CTGCAG	2 cut(s)	348, 1198
PsuI	RGATCY	1 cut(s)	710
PvuII	CAGCTG	1 cut(s)	695
RruI	TCGCGA	1 cut(s)	1382
RsaI	GTAC	4 cut(s)	117, 889, 1056, 1581
RsaNI	GTAC	4 cut(s)	116, 888, 1055, 1580
RseI	CAYNNNNRTG	1 cut(s)	2090
SacI	GAGCTC	2 cut(s)	1155, 1594
SapI	GCTCTTC	1 cut(s)	11
SatI	GCNGC	5 cut(s)	347, 436, 492, 949, 1218
Sau3AI	GATC	7 cut(s)	152, 710, 1178, 1200, 1618, 2065, 2112
Sau96I	GGNCC	4 cut(s)	196, 340, 665, 1907
ScaI	AGTACT	1 cut(s)	1056
SchI	GAGTC	6 cut(s)	70, 1282, 1550, 1739, 1841, 1994
ScrFI	CCNGG	2 cut(s)	178, 2027
SduI	GDGCHC	3 cut(s)	601, 1155, 1594
SfaNI	GCATC	8 cut(s)	811, 897, 910, 1179, 1204, 1816, 2066, 2090
SfcI	CTRYAG	2 cut(s)	344, 1194
Sfr274I	CTCGAG	1 cut(s)	335
SfuI	TTCGAA	1 cut(s)	449
SinI	GGWCC	4 cut(s)	196, 340, 665, 1907
SlaI	CTCGAG	1 cut(s)	335
SmiMI	CAYNNNNRTG	1 cut(s)	2090
SmlI	CTYRAG	3 cut(s)	278, 335, 1927
SmoI	CTYRAG	3 cut(s)	278, 335, 1927
SpeI	ACTAGT	1 cut(s)	980
SphI	GCATGC	1 cut(s)	1224
SseBI	AGGCCT	2 cut(s)	574, 1872
SsiI	CCGC	5 cut(s)	210, 291, 1656, 1679, 1866
SspI	AATATT	1 cut(s)	425
SspMI	CTAG	6 cut(s)	119, 707, 981, 1035, 1533, 1613
SstI	GAGCTC	2 cut(s)	1155, 1594
StuI	AGGCCT	2 cut(s)	574, 1872
StyD4I	CCNGG	2 cut(s)	176, 2025
StyI	CCWWGG	3 cut(s)	882, 1740, 1782
TaaI	ACNGT	7 cut(s)	130, 412, 476, 1249, 1765, 1999, 2109
TaiI	ACGT	1 cut(s)	405
TatI	WGTACW	3 cut(s)	115, 1054, 1579
TfiI	GAWTC	4 cut(s)	141, 1010, 1328, 1608
TscAI	CASTG	7 cut(s)	135, 601, 841, 1203, 1252, 1572, 1582
TseFI	GTSAC	7 cut(s)	286, 398, 769, 1045, 1285, 1563, 1986
TseI	GCWGC	5 cut(s)	346, 435, 491, 948, 1217
Tsp45I	GTSAC	7 cut(s)	286, 398, 769, 1045, 1285, 1563, 1986
TspDTI	ATGAA	4 cut(s)	41, 647, 730, 1496
TspGWI	ACGGA	2 cut(s)	1591, 1636
TspRI	CASTG	7 cut(s)	135, 601, 841, 1203, 1252, 1572, 1582
Vha464I	CTTAAG	1 cut(s)	1927
VpaK11BI	GGWCC	4 cut(s)	196, 340, 665, 1907
VspI	ATTAAT	2 cut(s)	1274, 1442
XapI	RAATTY	2 cut(s)	1844, 2021
XceI	RCATGY	3 cut(s)	193, 1192, 1224
XcmI	CCANNNNNNNNNTGG	1 cut(s)	540
XhoI	CTCGAG	1 cut(s)	335
XmiI	GTMKAC	1 cut(s)	1640
XmnI	GAANNNNTTC	2 cut(s)	753, 1844
XspI	CTAG	6 cut(s)	119, 707, 981, 1035, 1533, 1613
ZraI	GACGTC	1 cut(s)	403
ZrmI	AGTACT	1 cut(s)	1056
Zsp2I	ATGCAT	2 cut(s)	925, 1134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.