Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Reverse (-)

69170272 .. 69172876

Gene Structure 2605 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG460600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGAGTATTACACCGGCGTCGTCCCTGTTCAATAAGAGCTTGACTTGCGTGGAGTTACCGCCCAACATTCATTCAAGTGGTATGTGGCCGAAACTAAATGCTACTGCTGCAGAAGTTGAGTTTGTGACCACGAATGCTACATTGCCAATGCTCCAAAAGAAAATGCTTATGACCTTCTTAGCCACCCAGCTCTTCCATCTCATTCTCGGTTCTTTTGGCGTCCCATTCTTCGTCTCTCAAATGCTGGCAGGATTCTTACTTGGTCCAGCGGTGGTTGGCAGAATTAAATCCGACGAAAATTGGTACAATGACTGGATGTTGTCCTTGACAAGTCAGGACATACTTGGCACTCTCACAATGTTTGGCTTCTCCATGTTTTTGTTTCTGGCAGCAGTGAAGATGGATGCAAGCCTGTTATTGAAGACTGGGAGAAAGGCCTTTTACACTGGTGTATTCTCCTTGATAGTTCCCCTAATTGTTGGCTTTTCCACAGTTGGGTTGCTTGAATATCAGAAGGCGAAAATTAATCCGAAAGAGAAAAAAGGAGTGGCATCCGAAACTATATTCCTGGTATTATCAATCTGTGCAACATCATTTCCTGTCATAGTTTCCCTTCTTAGCCAACTCCGTATCCTTACTTCGGAACTCGGGCGATTAGGATTGTCAGCAGCTCTAGTGTGTGACATGCTAAAATTGATTCTCACTACTATTGCGAGAGCGTTTGGTCCAACCCTAGGTGCCAGAAAGTTTAGTTACAAGTCGTCTCTTGTTATCTTGGGATATAGTCTGGCTTGTGCGTTCGTGGTCCGGCCGGCTTTGAATTGGGTCATCAGACAGACACCTAAAGGGAAGCCTGTTAACAATGGCTATATCTTTGTCATCCTCCTGCTGGCCTTGGCTTCCGGAGCTCACGAGCAACTTGTTTTATTTGGACCTTTCTTTTTCGGCTTAGTTATACCAAGTGGACCTCCCCTCGGGTCGACCCTGGTTCAGAAATACGACTTCATGGTCTCGCGTGTGTTCATGCCACTGTTTGTCACCAATTGTGTGATCATGGCAGATGTCAGGGAACTCCGCTTCGATGCCCATTTACTAGTCTTGCTGGTAACTATGTTGGCCAAATTCTTGGGCACTTTAGCACCTGCACTCTGCTGTAGAATTCCTATCAGGGATGCTTTGGCACTCGCTTTCATACTGAGCTGCAAAGGTGCTGTTGAAATCGGTGGTTACTTCGTGATCTATGACGGCCAGCGTATCACCGACGGAATTTATGCTTTGGCGATGTTGATGATCCTATTCGCCCATTTCACCGTGGCATATGCTGTGAAGCACTTGTACAAGACATCGACTAGGTACGCAGGGTATCAGCAGAGAAACGTACTAGGCATGAGACCTAACTCGGAGCTCAAGATACTTTCCTGCATCTACAAACACGACAACGTGCCAGCTGTCATCAACTTATTGGATGTGGCTTGCCCTACCGGAGATAACCCCATTGGTCTCAACGTCCTCCACCTTATAGAGATGACCGGCCAAGACGCCCCCATGCTCATTTCCCACCGCATGCAGAAGAAGACAATGTCCAGCTTCTCCGACTCCGAGGACGTCCTCGTTTCTTTCCTTAATTTGGAGCGGGAGTACGGTGACGCCGTTGAGGTCCACCCCTACACCGCCGTATGGCCGTCAAAGTGGATGTACGAAGAGATATGCACACTCGCCCTGGACAGGCTCACACATCTCATCATACTCCCCTTCCACCGCCAGTGGACCGTGGACGGCGCCGTTAAATCGGAGGACGAAACCGTCAGGAACATAAACATTAGCGTTCTAGATGGATCGCCTTGTTCCGTCGGAGTTCTAGTCAACCGGGGCCCGCTCCGGCAAAACCACAGAACAGAAGCGTCGCAATCACAGTTCAACGTTGCGTTGATTTTCTTGGGTGGCGAAGACGATAGGGAGGCGTTGGCTTTGACGGGAAGGATGACCGCCGACCCAAACATCACCCTAACTGTGTTTCATATCAGTCCCACCACACTGCCTGAACATGGACCTAACTGGGAGAACATTCTAGACTTTGAGAGCTTAAGGAAGTTCAGGCAAAACAACACCGGCAACGGCCGTTACGTGATTTACACGGAGGCGGTTTCGGAGAGTGGGGAACAAACTGTTGACATGTTGAGGGAGATCGTGCACAAGTATGACCTTTTCGTGGTTGGGAGGAGGAAAGAGGTTAAGGATAGTCCTCAGACTTCAGGGCTTGAAGATTGGAGTGAGTTCCCGGAGATTGGGATTGTAGGTGACATGCTCGCATCGGATGATTTGGGTTGCAGATCTTCTATCTTGGTTGTGCAGAACCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

786

Amino Acids

86.97

Weight (kDa)

6.96

Isoelectric Point (pI)

39.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	68 - 436	1.4e-28	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	503 - 624	4.3e-07	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	640 - 785	1.9e-08	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1150
AasI	GACNNNNNNGTC	2 cut(s)	1007, 1802
AatII	GACGTC	1 cut(s)	1608
Acc36I	ACCTGC	1 cut(s)	1150
AccB1I	GGYRCC	2 cut(s)	737, 1778
AccBSI	CCGCTC	2 cut(s)	1633, 1876
AccI	GTMKAC	1 cut(s)	980
AccII	CGCG	1 cut(s)	1015
AccIII	TCCGGA	1 cut(s)	902
AclI	AACGTT	1 cut(s)	1920
AclWI	GGATC	2 cut(s)	1285, 1843
AcoI	YGGCCR	7 cut(s)	86, 809, 1116, 1246, 1531, 1679, 2116
AcsI	RAATTY	3 cut(s)	1121, 1158, 1266
AcuI	CTGAAG	1 cut(s)	2235
AcyI	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
AfaI	GTAC	6 cut(s)	305, 1337, 1355, 1380, 1640, 1697
AflII	CTTAAG	1 cut(s)	2083
AflIII	ACRYGT	1 cut(s)	2172
AgsI	TTSAA	8 cut(s)	31, 75, 421, 506, 820, 1217, 1918, 2261
AhlI	ACTAGT	1 cut(s)	1093
AjnI	CCWGG	3 cut(s)	567, 984, 1719
AluBI	AGCT	9 cut(s)	39, 190, 671, 908, 1200, 1405, 1448, 1587, 2082
AluI	AGCT	9 cut(s)	39, 190, 671, 908, 1200, 1405, 1448, 1587, 2082
Alw21I	GWGCWC	3 cut(s)	910, 1407, 2193
Alw26I	GTCTC	5 cut(s)	238, 768, 1015, 1384, 1505
Alw44I	GTGCAC	1 cut(s)	2189
AlwI	GGATC	2 cut(s)	1285, 1843
Ama87I	CYCGRG	2 cut(s)	647, 974
Aor13HI	TCCGGA	1 cut(s)	902
ApaI	GGGCCC	1 cut(s)	1874
ApaLI	GTGCAC	1 cut(s)	2189
ApeKI	GCWGC	4 cut(s)	107, 389, 668, 1200
ApoI	RAATTY	3 cut(s)	1121, 1158, 1266
AseI	ATTAAT	1 cut(s)	525
AspA2I	CCTAGG	1 cut(s)	733
AspLEI	GCGC	1 cut(s)	1781
AsuC2I	CCSGG	2 cut(s)	1868, 2279
AsuHPI	GGTGA	6 cut(s)	1030, 1249, 1300, 1655, 1993, 2309
AvaI	CYCGRG	2 cut(s)	647, 974
AvaII	GGWCC	8 cut(s)	263, 725, 805, 932, 965, 1657, 1767, 2048
AvrII	CCTAGG	1 cut(s)	733
BaeGI	GKGCMC	3 cut(s)	1133, 1874, 2193
BalI	TGGCCA	1 cut(s)	1118
BanI	GGYRCC	2 cut(s)	737, 1778
BanII	GRGCYC	3 cut(s)	910, 1407, 1874
BarI	GAAGNNNNNNTAC	4 cut(s)	622, 654, 1319, 1351
BauI	CACGAG	1 cut(s)	911
BbsI	GAAGAC	3 cut(s)	428, 1580, 1953
Bbv12I	GWGCWC	3 cut(s)	910, 1407, 2193
BbvI	GCAGC	4 cut(s)	94, 401, 680, 1187
BccI	CCATC	3 cut(s)	204, 394, 1826
BceAI	ACGGC	8 cut(s)	1261, 1634, 1658, 1666, 1766, 1792, 2103, 2131
BciT130I	CCWGG	3 cut(s)	569, 986, 1721
BciVI	GTATCC	1 cut(s)	641
BclI	TGATCA	1 cut(s)	1050
BcnI	CCSGG	2 cut(s)	1868, 2279
BcoDI	GTCTC	5 cut(s)	238, 768, 1015, 1384, 1505
BcuI	ACTAGT	1 cut(s)	1093
BfaI	CTAG	8 cut(s)	674, 734, 1094, 1350, 1382, 1829, 1859, 2069
BfmI	CTRYAG	2 cut(s)	108, 1153
BfoI	RGCGCY	1 cut(s)	1782
BfrI	CTTAAG	1 cut(s)	2083
BfuAI	ACCTGC	1 cut(s)	1150
BfuI	GTATCC	1 cut(s)	641
BglII	AGATCT	1 cut(s)	2330
BisI	GCNGC	4 cut(s)	108, 390, 669, 1201
BlnI	CCTAGG	1 cut(s)	733
BlsI	GCNGC	4 cut(s)	109, 391, 670, 1202
Bme1390I	CCNGG	5 cut(s)	569, 986, 1721, 1868, 2279
Bme18I	GGWCC	8 cut(s)	263, 725, 805, 932, 965, 1657, 1767, 2048
BmeT110I	CYCGRG	2 cut(s)	647, 974
BmiI	GGNNCC	4 cut(s)	739, 1780, 1871, 1872
BmrFI	CCNGG	5 cut(s)	569, 986, 1721, 1868, 2279
BmrI	ACTGGG	2 cut(s)	435, 2065
BmsI	GCATC	6 cut(s)	394, 560, 1072, 1162, 1431, 2318
BmuI	ACTGGG	2 cut(s)	435, 2065
BpiI	GAAGAC	3 cut(s)	428, 1580, 1953
BpuEI	CTTGAG	1 cut(s)	1391
BpuMI	CCSGG	2 cut(s)	1868, 2279
BsaAI	YACGTR	1 cut(s)	2125
BsaHI	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
BsaI	GGTCTC	3 cut(s)	1015, 1384, 1505
BsaJI	CCNNGG	9 cut(s)	733, 894, 973, 984, 1311, 1599, 1719, 1770, 1867
BsaWI	WCCGGW	2 cut(s)	902, 1481
BsaXI	ACNNNNNCTCC	4 cut(s)	1622, 1652, 1845, 1875
Bse118I	RCCGGY	4 cut(s)	13, 811, 1529, 2108
Bse1I	ACTGG	5 cut(s)	317, 430, 451, 1762, 2060
Bse3DI	GCAATG	1 cut(s)	140
BseAI	TCCGGA	1 cut(s)	902
BseBI	CCWGG	3 cut(s)	569, 986, 1721
BseDI	CCNNGG	9 cut(s)	733, 894, 973, 984, 1311, 1599, 1719, 1770, 1867
BseGI	GGATG	9 cut(s)	321, 409, 551, 879, 1177, 1471, 1698, 1986, 2320
BseMI	GCAATG	1 cut(s)	140
BseMII	CTCAG	2 cut(s)	1187, 2258
BseNI	ACTGG	5 cut(s)	317, 430, 451, 1762, 2060
BseRI	GAGGAG	1 cut(s)	2233
BseSI	GKGCMC	3 cut(s)	1133, 1874, 2193
BseX3I	CGGCCG	2 cut(s)	809, 2116
BseXI	GCAGC	4 cut(s)	94, 401, 680, 1187
BseYI	CCCAGC	1 cut(s)	186
BsgI	GTGCAG	1 cut(s)	1128
Bsh1236I	CGCG	1 cut(s)	1015
Bsh1285I	CGRYCG	2 cut(s)	812, 2119
BshNI	GGYRCC	2 cut(s)	737, 1778
BsiEI	CGRYCG	2 cut(s)	812, 2119
BsiHKAI	GWGCWC	3 cut(s)	910, 1407, 2193
BsiHKCI	CYCGRG	2 cut(s)	647, 974
BslFI	GGGAC	3 cut(s)	7, 206, 2010
BsmAI	GTCTC	5 cut(s)	238, 768, 1015, 1384, 1505
BsmBI	CGTCTC	2 cut(s)	238, 768
BsmFI	GGGAC	3 cut(s)	7, 206, 2010
BsmI	GAATGC	1 cut(s)	139
Bso31I	GGTCTC	3 cut(s)	1015, 1384, 1505
BsoBI	CYCGRG	2 cut(s)	647, 974
Bsp120I	GGGCCC	1 cut(s)	1870
Bsp1286I	GDGCHC	5 cut(s)	910, 1133, 1407, 1874, 2193
Bsp13I	TCCGGA	1 cut(s)	902
Bsp1407I	TGTACA	1 cut(s)	1335
Bsp143I	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
BspCNI	CTCAG	2 cut(s)	1188, 2257
BspEI	TCCGGA	1 cut(s)	902
BspFNI	CGCG	1 cut(s)	1015
BspLI	GGNNCC	4 cut(s)	739, 1780, 1871, 1872
BspMAI	CTGCAG	1 cut(s)	112
BspMI	ACCTGC	1 cut(s)	1150
BspPI	GGATC	2 cut(s)	1285, 1843
BspQI	GCTCTTC	1 cut(s)	197
BspT107I	GGYRCC	2 cut(s)	737, 1778
BspTI	CTTAAG	1 cut(s)	2083
BspTNI	GGTCTC	3 cut(s)	1015, 1384, 1505
BsrBI	CCGCTC	2 cut(s)	1633, 1876
BsrDI	GCAATG	1 cut(s)	140
BsrFI	RCCGGY	4 cut(s)	13, 811, 1529, 2108
BsrGI	TGTACA	1 cut(s)	1335
BsrI	ACTGG	5 cut(s)	317, 430, 451, 1762, 2060
BssAI	RCCGGY	4 cut(s)	13, 811, 1529, 2108
BssECI	CCNNGG	9 cut(s)	733, 894, 973, 984, 1311, 1599, 1719, 1770, 1867
BssMI	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
BssNI	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
BssSI	CACGAG	1 cut(s)	911
BssT1I	CCWWGG	2 cut(s)	733, 894
Bst2BI	CACGAG	1 cut(s)	911
Bst2UI	CCWGG	3 cut(s)	569, 986, 1721
Bst4CI	ACNGT	9 cut(s)	493, 1032, 1312, 1643, 1771, 1804, 1914, 2011, 2167
Bst6I	CTCTTC	2 cut(s)	197, 1695
BstACI	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
BstAFI	CTTAAG	1 cut(s)	2083
BstAUI	TGTACA	1 cut(s)	1335
BstBAI	YACGTR	1 cut(s)	2125
BstDEI	CTNAG	5 cut(s)	178, 617, 949, 1196, 2244
BstDSI	CCRYGG	2 cut(s)	1311, 1770
BstF5I	GGATG	9 cut(s)	321, 409, 551, 879, 1177, 1471, 1698, 1986, 2320
BstFNI	CGCG	1 cut(s)	1015
BstH2I	RGCGCY	1 cut(s)	1782
BstHHI	GCGC	1 cut(s)	1781
BstKTI	GATC	6 cut(s)	1053, 1239, 1293, 1838, 2187, 2333
BstMAI	GTCTC	5 cut(s)	238, 768, 1015, 1384, 1505
BstMBI	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
BstMCI	CGRYCG	2 cut(s)	812, 2119
BstMWI	GCNNNNNNNGC	3 cut(s)	45, 107, 905
BstNI	CCWGG	3 cut(s)	569, 986, 1721
BstNSI	RCATGY	4 cut(s)	688, 1567, 2176, 2305
BstSCI	CCNGG	5 cut(s)	567, 984, 1719, 1866, 2277
BstSFI	CTRYAG	2 cut(s)	108, 1153
BstSLI	GKGCMC	3 cut(s)	1133, 1874, 2193
BstUI	CGCG	1 cut(s)	1015
BstV1I	GCAGC	4 cut(s)	94, 401, 680, 1187
BstV2I	GAAGAC	3 cut(s)	428, 1580, 1953
BstX2I	RGATCY	1 cut(s)	2330
BstXI	CCANNNNNNTGG	1 cut(s)	1126
BstYI	RGATCY	1 cut(s)	2330
BstZI	CGGCCG	2 cut(s)	809, 2116
BsuI	GTATCC	1 cut(s)	641
BtgI	CCRYGG	2 cut(s)	1311, 1770
BtgZI	GCGATG	1 cut(s)	1295
BtsCI	GGATG	9 cut(s)	321, 409, 551, 879, 1177, 1471, 1698, 1986, 2320
BtsI	GCAGTG	2 cut(s)	399, 2033
BtsIMutI	CAGTG	5 cut(s)	399, 444, 1028, 1769, 2033
BveI	ACCTGC	1 cut(s)	1150
CfoI	GCGC	1 cut(s)	1781
Cfr10I	RCCGGY	4 cut(s)	13, 811, 1529, 2108
CseI	GACGC	5 cut(s)	6, 208, 1547, 1655, 1890
Csp6I	GTAC	6 cut(s)	304, 1336, 1354, 1379, 1639, 1696
CviQI	GTAC	6 cut(s)	304, 1336, 1354, 1379, 1639, 1696
DdeI	CTNAG	5 cut(s)	178, 617, 949, 1196, 2244
DinI	GGCGCC	1 cut(s)	1780
DpnI	GATC	6 cut(s)	1052, 1238, 1292, 1837, 2186, 2332
DpnII	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
DrdI	GACNNNNNNGTC	2 cut(s)	1007, 1802
DseDI	GACNNNNNNGTC	2 cut(s)	1007, 1802
EaeI	YGGCCR	7 cut(s)	86, 809, 1116, 1246, 1531, 1679, 2116
EagI	CGGCCG	2 cut(s)	809, 2116
Eam1104I	CTCTTC	2 cut(s)	197, 1695
EarI	CTCTTC	2 cut(s)	197, 1695
Ecl136II	GAGCTC	2 cut(s)	908, 1405
EclXI	CGGCCG	2 cut(s)	809, 2116
Eco130I	CCWWGG	2 cut(s)	733, 894
Eco147I	AGGCCT	1 cut(s)	437
Eco24I	GRGCYC	3 cut(s)	910, 1407, 1874
Eco31I	GGTCTC	3 cut(s)	1015, 1384, 1505
Eco47I	GGWCC	8 cut(s)	263, 725, 805, 932, 965, 1657, 1767, 2048
Eco52I	CGGCCG	2 cut(s)	809, 2116
Eco53kI	GAGCTC	2 cut(s)	908, 1405
Eco57I	CTGAAG	1 cut(s)	2235
Eco88I	CYCGRG	2 cut(s)	647, 974
EcoICRI	GAGCTC	2 cut(s)	908, 1405
EcoO109I	RGGNCCY	1 cut(s)	1870
EcoRI	GAATTC	1 cut(s)	1158
EcoRII	CCWGG	3 cut(s)	567, 984, 1719
EcoT14I	CCWWGG	2 cut(s)	733, 894
EcoT38I	GRGCYC	3 cut(s)	910, 1407, 1874
EgeI	GGCGCC	1 cut(s)	1780
EheI	GGCGCC	1 cut(s)	1780
ErhI	CCWWGG	2 cut(s)	733, 894
Esp3I	CGTCTC	2 cut(s)	238, 768
FaqI	GGGAC	3 cut(s)	7, 206, 2010
FauI	CCCGC	2 cut(s)	1626, 1881
FauNDI	CATATG	1 cut(s)	1318
FbaI	TGATCA	1 cut(s)	1050
FblI	GTMKAC	1 cut(s)	980
Fnu4HI	GCNGC	4 cut(s)	108, 390, 669, 1201
FokI	GGATG	9 cut(s)	328, 416, 538, 866, 1184, 1478, 1705, 1993, 2327
FriOI	GRGCYC	3 cut(s)	910, 1407, 1874
Fsp4HI	GCNGC	4 cut(s)	108, 390, 669, 1201
FspBI	CTAG	8 cut(s)	674, 734, 1094, 1350, 1382, 1829, 1859, 2069
GlaI	GCGC	1 cut(s)	1780
GluI	GCNGC	4 cut(s)	108, 390, 669, 1201
GsaI	CCCAGC	1 cut(s)	190
HaeII	RGCGCY	1 cut(s)	1782
HgaI	GACGC	5 cut(s)	6, 208, 1547, 1655, 1890
HhaI	GCGC	1 cut(s)	1781
Hin1I	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
Hin6I	GCGC	1 cut(s)	1779
HinP1I	GCGC	1 cut(s)	1779
HincII	GTYRAC	4 cut(s)	859, 981, 1864, 2170
HindII	GTYRAC	4 cut(s)	859, 981, 1864, 2170
HinfI	GANTC	3 cut(s)	252, 697, 1595
HpaI	GTTAAC	1 cut(s)	859
HphI	GGTGA	6 cut(s)	1030, 1249, 1300, 1655, 1993, 2309
Hpy166II	GTNNAC	9 cut(s)	859, 965, 981, 1660, 1767, 1774, 1864, 2170, 2191
Hpy188III	TCNNGA	8 cut(s)	335, 903, 911, 1234, 1408, 1807, 1829, 2069
Hpy8I	GTNNAC	9 cut(s)	859, 965, 981, 1660, 1767, 1774, 1864, 2170, 2191
Hpy99I	CGWCG	5 cut(s)	22, 296, 1265, 1853, 1906
HpyAV	CCTTC	5 cut(s)	184, 508, 623, 1762, 1971
HpyCH4III	ACNGT	9 cut(s)	493, 1032, 1312, 1643, 1771, 1804, 1914, 2011, 2167
HpyCH4IV	ACGT	6 cut(s)	1377, 1440, 1506, 1605, 1920, 2124
HpyF10VI	GCNNNNNNNGC	3 cut(s)	45, 107, 905
HpyF3I	CTNAG	5 cut(s)	178, 617, 949, 1196, 2244
HpySE526I	ACGT	6 cut(s)	1377, 1440, 1506, 1605, 1920, 2124
Hsp92I	GRCGYC	6 cut(s)	17, 219, 1539, 1605, 1647, 1779
HspAI	GCGC	1 cut(s)	1779
KasI	GGCGCC	1 cut(s)	1778
Kpn2I	TCCGGA	1 cut(s)	902
KroI	GCCGGC	1 cut(s)	811
KroNI	GCCGGC	1 cut(s)	813
Ksp22I	TGATCA	1 cut(s)	1050
KspAI	GTTAAC	1 cut(s)	859
Kzo9I	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
LguI	GCTCTTC	1 cut(s)	197
LmnI	GCTCC	5 cut(s)	156, 905, 1402, 1630, 1881
Lsp1109I	GCAGC	4 cut(s)	94, 401, 680, 1187
LweI	GCATC	6 cut(s)	394, 560, 1072, 1162, 1431, 2318
MaeI	CTAG	8 cut(s)	674, 734, 1094, 1350, 1382, 1829, 1859, 2069
MaeII	ACGT	6 cut(s)	1377, 1440, 1506, 1605, 1920, 2124
MalI	GATC	6 cut(s)	1052, 1238, 1292, 1837, 2186, 2332
MbiI	CCGCTC	2 cut(s)	1633, 1876
MboI	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
MfeI	CAATTG	1 cut(s)	1042
MflI	RGATCY	1 cut(s)	2330
MhlI	GDGCHC	5 cut(s)	910, 1133, 1407, 1874, 2193
MlsI	TGGCCA	1 cut(s)	1118
MluNI	TGGCCA	1 cut(s)	1118
Mly113I	GGCGCC	1 cut(s)	1779
MlyI	GAGTC	1 cut(s)	1589
MmeI	TCCRAC	4 cut(s)	315, 752, 1617, 1831
Mox20I	TGGCCA	1 cut(s)	1118
MroI	TCCGGA	1 cut(s)	902
MroNI	GCCGGC	1 cut(s)	811
MscI	TGGCCA	1 cut(s)	1118
MseI	TTAA	7 cut(s)	285, 525, 858, 1623, 1785, 2084, 2232
MslI	CAYNNNNRTG	2 cut(s)	75, 2196
Msp20I	TGGCCA	1 cut(s)	1118
MspA1I	CMGCKG	2 cut(s)	269, 1448
MspCI	CTTAAG	1 cut(s)	2083
MspR9I	CCNGG	5 cut(s)	569, 986, 1721, 1868, 2279
MunI	CAATTG	1 cut(s)	1042
Mva1269I	GAATGC	1 cut(s)	139
MvaI	CCWGG	3 cut(s)	569, 986, 1721
MvnI	CGCG	1 cut(s)	1015
MwoI	GCNNNNNNNGC	3 cut(s)	45, 107, 905
NaeI	GCCGGC	1 cut(s)	813
NarI	GGCGCC	1 cut(s)	1779
NciI	CCSGG	2 cut(s)	1868, 2279
NdeI	CATATG	1 cut(s)	1318
NdeII	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
NgoMIV	GCCGGC	1 cut(s)	811
NlaIV	GGNNCC	4 cut(s)	739, 1780, 1871, 1872
NmuCI	GTSAC	5 cut(s)	124, 680, 1036, 1643, 2297
NspI	RCATGY	4 cut(s)	688, 1567, 2176, 2305
PaeI	GCATGC	1 cut(s)	1567
PaqCI	CACCTGC	1 cut(s)	1150
PceI	AGGCCT	1 cut(s)	437
PciI	ACATGT	1 cut(s)	2172
PciSI	GCTCTTC	1 cut(s)	197
PcsI	WCGNNNNNNNCGW	2 cut(s)	1647, 2121
PctI	GAATGC	1 cut(s)	139
PdiI	GCCGGC	1 cut(s)	813
PfeI	GAWTC	2 cut(s)	252, 697
PflFI	GACNNNGTC	1 cut(s)	1579
PfoI	TCCNGGA	1 cut(s)	2277
PkrI	GCNGC	4 cut(s)	109, 391, 670, 1202
PleI	GAGTC	1 cut(s)	1589
PluTI	GGCGCC	1 cut(s)	1782
PpsI	GAGTC	1 cut(s)	1589
Ppu21I	YACGTR	1 cut(s)	2125
PscI	ACATGT	1 cut(s)	2172
PshBI	ATTAAT	1 cut(s)	525
Psp124BI	GAGCTC	2 cut(s)	910, 1407
Psp1406I	AACGTT	1 cut(s)	1920
Psp6I	CCWGG	3 cut(s)	567, 984, 1719
PspFI	CCCAGC	1 cut(s)	186
PspGI	CCWGG	3 cut(s)	567, 984, 1719
PspN4I	GGNNCC	4 cut(s)	739, 1780, 1871, 1872
PspOMI	GGGCCC	1 cut(s)	1870
PstI	CTGCAG	1 cut(s)	112
PsuI	RGATCY	1 cut(s)	2330
PsyI	GACNNNGTC	1 cut(s)	1579
PvuII	CAGCTG	1 cut(s)	1448
RsaI	GTAC	6 cut(s)	305, 1337, 1355, 1380, 1640, 1697
RsaNI	GTAC	6 cut(s)	304, 1336, 1354, 1379, 1639, 1696
RseI	CAYNNNNRTG	2 cut(s)	75, 2196
SacI	GAGCTC	2 cut(s)	910, 1407
SalI	GTCGAC	1 cut(s)	979
SapI	GCTCTTC	1 cut(s)	197
SaqAI	TTAA	7 cut(s)	285, 525, 858, 1623, 1785, 2084, 2232
SatI	GCNGC	4 cut(s)	108, 390, 669, 1201
Sau3AI	GATC	6 cut(s)	1050, 1236, 1290, 1835, 2184, 2330
SchI	GAGTC	1 cut(s)	1589
ScrFI	CCNGG	5 cut(s)	569, 986, 1721, 1868, 2279
SduI	GDGCHC	5 cut(s)	910, 1133, 1407, 1874, 2193
SfaNI	GCATC	6 cut(s)	394, 560, 1072, 1162, 1431, 2318
SfcI	CTRYAG	2 cut(s)	108, 1153
SfoI	GGCGCC	1 cut(s)	1780
SgrAI	CRCCGGYG	1 cut(s)	13
SinI	GGWCC	8 cut(s)	263, 725, 805, 932, 965, 1657, 1767, 2048
SmiMI	CAYNNNNRTG	2 cut(s)	75, 2196
SmlI	CTYRAG	2 cut(s)	1406, 2083
SmoI	CTYRAG	2 cut(s)	1406, 2083
SpeI	ACTAGT	1 cut(s)	1093
SphI	GCATGC	1 cut(s)	1567
SseBI	AGGCCT	1 cut(s)	437
SspDI	GGCGCC	1 cut(s)	1778
SspMI	CTAG	8 cut(s)	674, 734, 1094, 1350, 1382, 1829, 1859, 2069
SstI	GAGCTC	2 cut(s)	910, 1407
StuI	AGGCCT	1 cut(s)	437
StyD4I	CCNGG	5 cut(s)	567, 984, 1719, 1866, 2277
StyI	CCWWGG	2 cut(s)	733, 894
TaaI	ACNGT	9 cut(s)	493, 1032, 1312, 1643, 1771, 1804, 1914, 2011, 2167
TaiI	ACGT	6 cut(s)	1380, 1443, 1509, 1608, 1923, 2127
TaqI	TCGA	3 cut(s)	980, 1080, 1346
TatI	WGTACW	1 cut(s)	1335
TfiI	GAWTC	2 cut(s)	252, 697
Tru1I	TTAA	7 cut(s)	285, 525, 858, 1623, 1785, 2084, 2232
Tru9I	TTAA	7 cut(s)	285, 525, 858, 1623, 1785, 2084, 2232
TscAI	CASTG	5 cut(s)	399, 451, 1035, 1769, 2040
TseFI	GTSAC	5 cut(s)	124, 680, 1036, 1643, 2297
TseI	GCWGC	4 cut(s)	107, 389, 668, 1200
Tsp45I	GTSAC	5 cut(s)	124, 680, 1036, 1643, 2297
TspDTI	ATGAA	5 cut(s)	59, 994, 1012, 1180, 2006
TspGWI	ACGGA	4 cut(s)	617, 1278, 1837, 2150
TspRI	CASTG	5 cut(s)	399, 451, 1035, 1769, 2040
Tth111I	GACNNNGTC	1 cut(s)	1579
Vha464I	CTTAAG	1 cut(s)	2083
VneI	GTGCAC	1 cut(s)	2189
VpaK11BI	GGWCC	8 cut(s)	263, 725, 805, 932, 965, 1657, 1767, 2048
VspI	ATTAAT	1 cut(s)	525
XapI	RAATTY	3 cut(s)	1121, 1158, 1266
XbaI	TCTAGA	2 cut(s)	1828, 2068
XceI	RCATGY	4 cut(s)	688, 1567, 2176, 2305
XmaJI	CCTAGG	1 cut(s)	733
XmiI	GTMKAC	1 cut(s)	980
XspI	CTAG	8 cut(s)	674, 734, 1094, 1350, 1382, 1829, 1859, 2069
ZraI	GACGTC	1 cut(s)	1606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.