Rw4G037110

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001192
Physical Location & Seq
Reverse (-)
63841459 .. 63844107
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037110.1

Sequence Viewer

Length: 2370 bp
ATGAGTATTACACCGGCGTCGTCCCTGTTCAATAAGAGCTTGACTTGCGTGGAGTTACCGCCCAACATTCATTCAAGTGGTATGTGGCAGAAACTAGATGCTACTGCTGCAGAAGATGAGTTTAACACCACGAGTGCTACATTGCCAATGCTCCAAAAGAAAATGCTTATGACCTTCTTAGCCACCCAGCTCTTCCATCTCATTCTCGGTTCTTTTGGCGTCCCATTCTTCGTCTCTCAAATGCTGGCAGGATTCTTACTTGGTCCAGCGGTGGTTGGCAGAATTAAATTCAACGAAAATTGGTACAATGACTGGATGTTGTCCTTGACAAGTCAGGACATACTTGGCACTCTCACAATGTTTGGCTTCTCCATGTTTTTGTTTCTGGCAGCAGTGAAGATGGATGCAAGCCTGTTATTGAAGACTGGGAGAAAGGCCTTTTACACTGGTGTATTCTCCTTGATAGTTCCCCTAATTGTTGGCTTTTCCACAGTTGGGTTGCTTGAATTAGTTGAATATCAGAAGGCGAAAATTAATCCGAAAGAGAAAAAAATAGTGGCACCCGAAACTATTTTCCTGGTATTATCAATCTGTGCAACATCATTTCCTGTCATAGTTTCCCTTCTTAGCCAACTCGGTATCCTTACTTCGGAACTCGGGCGATTAGGATTGTCAGCAGCTCTAGTGTGTGACATGCTAAAATTGATTCTCACTACTATTGCGAGAGCGTTTGGTCCAACCGTAACTGCCAGAAAGTTTAGTTCCAAGTCGGCTCTTGTTATCCTGGGATATAGTCTGGCTTGTGCGTTCGTGGCCCGGCCGGCTTTGAATTGGGTCATCAGACAGACACCTAAAGGGAAGCCTGTTAACAATGGCTACATTTTTGTCATCCTCCTGCTGGCCTTGGTCTCCGGAGCTCACAAGCAACTTGTTTTATTTGGACCTTTCTTTTTCGGCTTAGTTATACCAAGTGGACCTCCCCTCGGGTCGACCCTGGTTCAGAAATACGACTTCATGGTCTCGCGTGTGTTCATGCCACTGTTTGTCACCAATTGTGTGATCATGGCAGATGTCAGGGAACTCCGCTTCGATGGCCATTTACTAGTCTTGCTGGTAACTATGTTGGCCAAATTCTTGGGCACTTTAGCACCTGCACTCTGCTGTAGAATTCCTATCAGGGATGCTTTGGCACTCGCTTTCATACTGAGCTGCAAAGGTGCTGTTGAAATCGGTGGTTACTTCGTGATCTATGACGGCCAGCGTATCACCGACGGAATTTATGCTTTGGCGATGTTGATGATCCTATTCGCCCATTTCACCGTGGCATATGCTGTGAAGCACTTGTACAAGACATCGACTAGGTACGCAGGGTATCAGCAGAGAAACGTACTAGGCATGAGACCTAACTCGGAGCTCAAGATACTTTCCTGCATCTACAAACAAGACAACGTGCCAGCTGTCATCAACTTATTGGATGTGGCTTGCCCTACCGGAGATAACCCCATTGGTCTCAACGTCCTCCACCTTATAGAGATGACCGGCCAAGACGCCCCCATGCTCATTTCCCACCGCATGCAGAAGAAGACAATGTCCAGCTTCTCCTACTCCGAGGACGTCCTCGTTTCTTTCCTTAATTTGGAGCGGGAGTACGGTGACGCCGTTGAGGTTCACCCCTACACCGCCGTATGGCCGTCAAAGTGGATGTACGAAGAGATATGCACACTCGCCCTAGACAGGCTCACACATCTCATCATACTCCCCTTCCACCGCCAGTGGACCGTGGACGGCGCCGTTAAATCGGAGGACGAAACTGTCAGGAACATAAACATTAGCGTTCTAGATGGATCACCTTGTTCCGTAGGAGTCCTAGTCAACCGGGGCCCGCTCCGGCAAAACCACAGAACAGAAGCGTCGCAATCACAGTTCAACGTTGCGTTGATTTTCTTGGGTGGCGAAGACGATAGGGAGGCGTTGGCTTTGACGGGAAGGATGACCGCCGACCCAAACATCACCCTAACCGTGTTTCATATCAGTCCCACCACACTGCCTGAAAATGGACCTAACTGGGAGAACATTCTGGACTTTGAGAGCTTAAGGAAGTTCAGGCAAAACAACACCGGCAACGGCCGTTACGTGATTTACACGGAGGCGGTTTCGGAGAGTGGGGAACAAACTGTTGACATGTTGAGGGAGATCGTGCACAAGTATGACCTTTTCGTGGTTGGGAGGAGGAAAGAGGTTAAGGATAGTCCTCAGACTTCAGGGCTTGAAGATTGGAGTGAGTTCCCGGAGCTTGGGATTGTAGGTGACATGCTCGCATCGGATGATTTGGGTTGCAGATCTTCCATCTTGGTGGTGCAGAACCAATGA

Protein Analysis

789

Amino Acids

87.31

Weight (kDa)

6.92

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 68 - 439 2.6e-26 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 506 - 627 1.2e-06 Plant cation/H(+) antiporter domain
CHX17_C PF23259 643 - 788 9.7e-09 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1159
AasI GACNNNNNNGTC 2 cut(s) 1016, 1811
AatII GACGTC 1 cut(s) 1617
Acc36I ACCTGC 1 cut(s) 1159
AccB1I GGYRCC 2 cut(s) 559, 1787
AccBSI CCGCTC 2 cut(s) 1642, 1885
AccI GTMKAC 1 cut(s) 989
AccII CGCG 1 cut(s) 1024
AccIII TCCGGA 1 cut(s) 911
AclI AACGTT 1 cut(s) 1929
AclWI GGATC 2 cut(s) 1294, 1852
AcoI YGGCCR 7 cut(s) 818, 1093, 1125, 1255, 1540, 1688, 2125
AcsI RAATTY 4 cut(s) 287, 1130, 1167, 1275
AcuI CTGAAG 1 cut(s) 2244
AcyI GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
AfaI GTAC 6 cut(s) 305, 1346, 1364, 1389, 1649, 1706
AflII CTTAAG 1 cut(s) 2092
AflIII ACRYGT 1 cut(s) 2181
AhlI ACTAGT 1 cut(s) 1102
AjnI CCWGG 3 cut(s) 576, 783, 993
Alw21I GWGCWC 3 cut(s) 919, 1416, 2202
Alw26I GTCTC 5 cut(s) 238, 913, 1024, 1393, 1514
Alw44I GTGCAC 1 cut(s) 2198
AlwI GGATC 2 cut(s) 1294, 1852
Ama87I CYCGRG 2 cut(s) 656, 983
Aor13HI TCCGGA 1 cut(s) 911
ApaI GGGCCC 1 cut(s) 1883
ApaLI GTGCAC 1 cut(s) 2198
ApeKI GCWGC 4 cut(s) 107, 389, 677, 1209
ApoI RAATTY 4 cut(s) 287, 1130, 1167, 1275
AseI ATTAAT 1 cut(s) 534
AspLEI GCGC 1 cut(s) 1790
AspS9I GGNCC 9 cut(s) 263, 734, 814, 941, 974, 1776, 1879, 1880, 2057
AsuC2I CCSGG 3 cut(s) 817, 1877, 2288
AsuHPI GGTGA 8 cut(s) 1039, 1258, 1309, 1661, 1664, 1839, 2002, 2318
AvaI CYCGRG 2 cut(s) 656, 983
AvaII GGWCC 6 cut(s) 263, 734, 941, 974, 1776, 2057
BaeGI GKGCMC 3 cut(s) 1142, 1883, 2202
BalI TGGCCA 2 cut(s) 1095, 1127
BanI GGYRCC 2 cut(s) 559, 1787
BanII GRGCYC 3 cut(s) 919, 1416, 1883
BarI GAAGNNNNNNTAC 4 cut(s) 631, 663, 1328, 1360
BauI CACGAG 1 cut(s) 130
BbsI GAAGAC 3 cut(s) 428, 1589, 1962
Bbv12I GWGCWC 3 cut(s) 919, 1416, 2202
BbvI GCAGC 4 cut(s) 94, 401, 689, 1196
BccI CCATC 5 cut(s) 204, 394, 1085, 1835, 2354
BceAI ACGGC 8 cut(s) 1270, 1643, 1667, 1675, 1775, 1801, 2112, 2140
BciT130I CCWGG 3 cut(s) 578, 785, 995
BciVI GTATCC 1 cut(s) 650
BclI TGATCA 1 cut(s) 1059
BcnI CCSGG 3 cut(s) 817, 1877, 2288
BcoDI GTCTC 5 cut(s) 238, 913, 1024, 1393, 1514
BcuI ACTAGT 1 cut(s) 1102
BfaI CTAG 8 cut(s) 95, 683, 1103, 1359, 1391, 1730, 1838, 1868
BfmI CTRYAG 2 cut(s) 108, 1162
BfoI RGCGCY 1 cut(s) 1791
BfrI CTTAAG 1 cut(s) 2092
BfuAI ACCTGC 1 cut(s) 1159
BfuI GTATCC 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 821
BglII AGATCT 1 cut(s) 2339
BisI GCNGC 4 cut(s) 108, 390, 678, 1210
BlsI GCNGC 4 cut(s) 109, 391, 679, 1211
Bme1390I CCNGG 6 cut(s) 578, 785, 817, 995, 1877, 2288
Bme18I GGWCC 6 cut(s) 263, 734, 941, 974, 1776, 2057
BmeT110I CYCGRG 2 cut(s) 656, 983
BmgT120I GGNCC 9 cut(s) 263, 734, 814, 941, 974, 1776, 1879, 1880, 2057
BmiI GGNNCC 4 cut(s) 561, 1789, 1880, 1881
BmrFI CCNGG 6 cut(s) 578, 785, 817, 995, 1877, 2288
BmrI ACTGGG 2 cut(s) 435, 2074
BmsI GCATC 5 cut(s) 88, 394, 1171, 1440, 2327
BmuI ACTGGG 2 cut(s) 435, 2074
BpiI GAAGAC 3 cut(s) 428, 1589, 1962
BpuEI CTTGAG 1 cut(s) 1400
BpuMI CCSGG 3 cut(s) 817, 1877, 2288
BsaAI YACGTR 1 cut(s) 2134
BsaHI GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
BsaI GGTCTC 4 cut(s) 913, 1024, 1393, 1514
BsaJI CCNNGG 8 cut(s) 784, 903, 982, 993, 1320, 1608, 1779, 1876
BsaWI WCCGGW 2 cut(s) 911, 1490
BsaXI ACNNNNNCTCC 4 cut(s) 1631, 1661, 1854, 1884
Bse118I RCCGGY 4 cut(s) 13, 820, 1538, 2117
Bse1I ACTGG 5 cut(s) 317, 430, 451, 1771, 2069
Bse3DI GCAATG 1 cut(s) 140
BseAI TCCGGA 1 cut(s) 911
BseBI CCWGG 3 cut(s) 578, 785, 995
BseDI CCNNGG 8 cut(s) 784, 903, 982, 993, 1320, 1608, 1779, 1876
BseGI GGATG 8 cut(s) 321, 409, 888, 1186, 1480, 1707, 1995, 2329
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 2 cut(s) 1196, 2267
BseNI ACTGG 5 cut(s) 317, 430, 451, 1771, 2069
BseRI GAGGAG 1 cut(s) 2242
BseSI GKGCMC 3 cut(s) 1142, 1883, 2202
BseX3I CGGCCG 2 cut(s) 818, 2125
BseXI GCAGC 4 cut(s) 94, 401, 689, 1196
BseYI CCCAGC 1 cut(s) 186
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 1024
Bsh1285I CGRYCG 2 cut(s) 821, 2128
BshNI GGYRCC 2 cut(s) 559, 1787
BsiEI CGRYCG 2 cut(s) 821, 2128
BsiHKAI GWGCWC 3 cut(s) 919, 1416, 2202
BsiHKCI CYCGRG 2 cut(s) 656, 983
BslFI GGGAC 3 cut(s) 7, 206, 2019
BsmAI GTCTC 5 cut(s) 238, 913, 1024, 1393, 1514
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 3 cut(s) 7, 206, 2019
Bso31I GGTCTC 4 cut(s) 913, 1024, 1393, 1514
BsoBI CYCGRG 2 cut(s) 656, 983
Bsp120I GGGCCC 1 cut(s) 1879
Bsp1286I GDGCHC 5 cut(s) 919, 1142, 1416, 1883, 2202
Bsp13I TCCGGA 1 cut(s) 911
Bsp1407I TGTACA 1 cut(s) 1344
Bsp143I GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
BspCNI CTCAG 2 cut(s) 1197, 2266
BspEI TCCGGA 1 cut(s) 911
BspFNI CGCG 1 cut(s) 1024
BspLI GGNNCC 4 cut(s) 561, 1789, 1880, 1881
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 1159
BspPI GGATC 2 cut(s) 1294, 1852
BspQI GCTCTTC 1 cut(s) 197
BspT107I GGYRCC 2 cut(s) 559, 1787
BspTI CTTAAG 1 cut(s) 2092
BspTNI GGTCTC 4 cut(s) 913, 1024, 1393, 1514
BsrBI CCGCTC 2 cut(s) 1642, 1885
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 4 cut(s) 13, 820, 1538, 2117
BsrGI TGTACA 1 cut(s) 1344
BsrI ACTGG 5 cut(s) 317, 430, 451, 1771, 2069
BssAI RCCGGY 4 cut(s) 13, 820, 1538, 2117
BssECI CCNNGG 8 cut(s) 784, 903, 982, 993, 1320, 1608, 1779, 1876
BssMI GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
BssNI GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 903
Bst2BI CACGAG 1 cut(s) 130
Bst2UI CCWGG 3 cut(s) 578, 785, 995
Bst6I CTCTTC 2 cut(s) 197, 1704
BstACI GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
BstAFI CTTAAG 1 cut(s) 2092
BstAUI TGTACA 1 cut(s) 1344
BstBAI YACGTR 1 cut(s) 2134
BstDEI CTNAG 5 cut(s) 178, 626, 958, 1205, 2253
BstDSI CCRYGG 2 cut(s) 1320, 1779
BstF5I GGATG 8 cut(s) 321, 409, 888, 1186, 1480, 1707, 1995, 2329
BstFNI CGCG 1 cut(s) 1024
BstH2I RGCGCY 1 cut(s) 1791
BstHHI GCGC 1 cut(s) 1790
BstKTI GATC 6 cut(s) 1062, 1248, 1302, 1847, 2196, 2342
BstMAI GTCTC 5 cut(s) 238, 913, 1024, 1393, 1514
BstMBI GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
BstMCI CGRYCG 2 cut(s) 821, 2128
BstMWI GCNNNNNNNGC 5 cut(s) 45, 107, 812, 821, 1092
BstNI CCWGG 3 cut(s) 578, 785, 995
BstNSI RCATGY 4 cut(s) 697, 1576, 2185, 2314
BstSCI CCNGG 6 cut(s) 576, 783, 815, 993, 1875, 2286
BstSFI CTRYAG 2 cut(s) 108, 1162
BstSLI GKGCMC 3 cut(s) 1142, 1883, 2202
BstUI CGCG 1 cut(s) 1024
BstV1I GCAGC 4 cut(s) 94, 401, 689, 1196
BstV2I GAAGAC 3 cut(s) 428, 1589, 1962
BstX2I RGATCY 1 cut(s) 2339
BstXI CCANNNNNNTGG 2 cut(s) 1135, 2353
BstYI RGATCY 1 cut(s) 2339
BstZI CGGCCG 2 cut(s) 818, 2125
BsuI GTATCC 1 cut(s) 650
BtgI CCRYGG 2 cut(s) 1320, 1779
BtgZI GCGATG 1 cut(s) 1304
BtsCI GGATG 8 cut(s) 321, 409, 888, 1186, 1480, 1707, 1995, 2329
BtsI GCAGTG 2 cut(s) 399, 2042
BtsIMutI CAGTG 5 cut(s) 399, 444, 1037, 1778, 2042
BveI ACCTGC 1 cut(s) 1159
CfoI GCGC 1 cut(s) 1790
Cfr10I RCCGGY 4 cut(s) 13, 820, 1538, 2117
Cfr13I GGNCC 9 cut(s) 263, 734, 814, 941, 974, 1776, 1879, 1880, 2057
CseI GACGC 5 cut(s) 6, 208, 1556, 1664, 1899
Csp6I GTAC 6 cut(s) 304, 1345, 1363, 1388, 1648, 1705
CviQI GTAC 6 cut(s) 304, 1345, 1363, 1388, 1648, 1705
DdeI CTNAG 5 cut(s) 178, 626, 958, 1205, 2253
DinI GGCGCC 1 cut(s) 1789
DpnI GATC 6 cut(s) 1061, 1247, 1301, 1846, 2195, 2341
DpnII GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
DrdI GACNNNNNNGTC 2 cut(s) 1016, 1811
DseDI GACNNNNNNGTC 2 cut(s) 1016, 1811
EaeI YGGCCR 7 cut(s) 818, 1093, 1125, 1255, 1540, 1688, 2125
EagI CGGCCG 2 cut(s) 818, 2125
Eam1104I CTCTTC 2 cut(s) 197, 1704
EarI CTCTTC 2 cut(s) 197, 1704
Ecl136II GAGCTC 2 cut(s) 917, 1414
EclXI CGGCCG 2 cut(s) 818, 2125
Eco130I CCWWGG 1 cut(s) 903
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 3 cut(s) 919, 1416, 1883
Eco31I GGTCTC 4 cut(s) 913, 1024, 1393, 1514
Eco47I GGWCC 6 cut(s) 263, 734, 941, 974, 1776, 2057
Eco52I CGGCCG 2 cut(s) 818, 2125
Eco53kI GAGCTC 2 cut(s) 917, 1414
Eco57I CTGAAG 1 cut(s) 2244
Eco88I CYCGRG 2 cut(s) 656, 983
EcoICRI GAGCTC 2 cut(s) 917, 1414
EcoO109I RGGNCCY 1 cut(s) 1879
EcoRI GAATTC 1 cut(s) 1167
EcoRII CCWGG 3 cut(s) 576, 783, 993
EcoT14I CCWWGG 1 cut(s) 903
EcoT38I GRGCYC 3 cut(s) 919, 1416, 1883
EgeI GGCGCC 1 cut(s) 1789
EheI GGCGCC 1 cut(s) 1789
ErhI CCWWGG 1 cut(s) 903
Esp3I CGTCTC 1 cut(s) 238
FaqI GGGAC 3 cut(s) 7, 206, 2019
FauI CCCGC 2 cut(s) 1635, 1890
FauNDI CATATG 1 cut(s) 1327
FbaI TGATCA 1 cut(s) 1059
FblI GTMKAC 1 cut(s) 989
Fnu4HI GCNGC 4 cut(s) 108, 390, 678, 1210
FokI GGATG 8 cut(s) 328, 416, 875, 1193, 1487, 1714, 2002, 2336
FriOI GRGCYC 3 cut(s) 919, 1416, 1883
Fsp4HI GCNGC 4 cut(s) 108, 390, 678, 1210
FspBI CTAG 8 cut(s) 95, 683, 1103, 1359, 1391, 1730, 1838, 1868
GlaI GCGC 1 cut(s) 1789
GluI GCNGC 4 cut(s) 108, 390, 678, 1210
GsaI CCCAGC 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 1791
HgaI GACGC 5 cut(s) 6, 208, 1556, 1664, 1899
HhaI GCGC 1 cut(s) 1790
Hin1I GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
Hin6I GCGC 1 cut(s) 1788
HinP1I GCGC 1 cut(s) 1788
HincII GTYRAC 4 cut(s) 868, 990, 1873, 2179
HindII GTYRAC 4 cut(s) 868, 990, 1873, 2179
HinfI GANTC 3 cut(s) 252, 706, 1863
HpaI GTTAAC 1 cut(s) 868
HphI GGTGA 8 cut(s) 1039, 1258, 1309, 1661, 1664, 1839, 2002, 2318
Hpy166II GTNNAC 9 cut(s) 868, 974, 990, 1669, 1776, 1783, 1873, 2179, 2200
Hpy188III TCNNGA 7 cut(s) 335, 912, 1243, 1417, 1816, 1838, 2078
Hpy8I GTNNAC 9 cut(s) 868, 974, 990, 1669, 1776, 1783, 1873, 2179, 2200
Hpy99I CGWCG 3 cut(s) 22, 1274, 1915
HpyAV CCTTC 5 cut(s) 184, 517, 632, 1771, 1980
HpyCH4IV ACGT 6 cut(s) 1386, 1449, 1515, 1614, 1929, 2133
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 107, 812, 821, 1092
HpyF3I CTNAG 5 cut(s) 178, 626, 958, 1205, 2253
HpySE526I ACGT 6 cut(s) 1386, 1449, 1515, 1614, 1929, 2133
Hsp92I GRCGYC 6 cut(s) 17, 219, 1548, 1614, 1656, 1788
HspAI GCGC 1 cut(s) 1788
KasI GGCGCC 1 cut(s) 1787
Kpn2I TCCGGA 1 cut(s) 911
KroI GCCGGC 1 cut(s) 820
KroNI GCCGGC 1 cut(s) 822
Ksp22I TGATCA 1 cut(s) 1059
KspAI GTTAAC 1 cut(s) 868
Kzo9I GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 6 cut(s) 156, 914, 1411, 1639, 1890, 2290
Lsp1109I GCAGC 4 cut(s) 94, 401, 689, 1196
LweI GCATC 5 cut(s) 88, 394, 1171, 1440, 2327
MaeI CTAG 8 cut(s) 95, 683, 1103, 1359, 1391, 1730, 1838, 1868
MaeII ACGT 6 cut(s) 1386, 1449, 1515, 1614, 1929, 2133
MaeIII GTNAC 9 cut(s) 54, 689, 742, 1045, 1114, 1235, 1652, 2129, 2306
MalI GATC 6 cut(s) 1061, 1247, 1301, 1846, 2195, 2341
MbiI CCGCTC 2 cut(s) 1642, 1885
MboI GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
MfeI CAATTG 1 cut(s) 1051
MflI RGATCY 1 cut(s) 2339
MhlI GDGCHC 5 cut(s) 919, 1142, 1416, 1883, 2202
MlsI TGGCCA 2 cut(s) 1095, 1127
MluNI TGGCCA 2 cut(s) 1095, 1127
Mly113I GGCGCC 1 cut(s) 1788
MlyI GAGTC 1 cut(s) 1872
MmeI TCCRAC 1 cut(s) 761
Mox20I TGGCCA 2 cut(s) 1095, 1127
MroI TCCGGA 1 cut(s) 911
MroNI GCCGGC 1 cut(s) 820
MscI TGGCCA 2 cut(s) 1095, 1127
MseI TTAA 8 cut(s) 123, 285, 534, 867, 1632, 1794, 2093, 2241
MslI CAYNNNNRTG 3 cut(s) 75, 2205, 2351
Msp20I TGGCCA 2 cut(s) 1095, 1127
MspA1I CMGCKG 2 cut(s) 269, 1457
MspCI CTTAAG 1 cut(s) 2092
MspR9I CCNGG 6 cut(s) 578, 785, 817, 995, 1877, 2288
MunI CAATTG 1 cut(s) 1051
MvaI CCWGG 3 cut(s) 578, 785, 995
MvnI CGCG 1 cut(s) 1024
MwoI GCNNNNNNNGC 5 cut(s) 45, 107, 812, 821, 1092
NaeI GCCGGC 1 cut(s) 822
NarI GGCGCC 1 cut(s) 1788
NciI CCSGG 3 cut(s) 817, 1877, 2288
NdeI CATATG 1 cut(s) 1327
NdeII GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
NgoMIV GCCGGC 1 cut(s) 820
NlaIV GGNNCC 4 cut(s) 561, 1789, 1880, 1881
NmuCI GTSAC 4 cut(s) 689, 1045, 1652, 2306
NspI RCATGY 4 cut(s) 697, 1576, 2185, 2314
PaeI GCATGC 1 cut(s) 1576
PaqCI CACCTGC 1 cut(s) 1159
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 2181
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 2 cut(s) 1656, 2130
PdiI GCCGGC 1 cut(s) 822
PfeI GAWTC 2 cut(s) 252, 706
PflFI GACNNNGTC 1 cut(s) 1588
PfoI TCCNGGA 1 cut(s) 2286
PkrI GCNGC 4 cut(s) 109, 391, 679, 1211
PleI GAGTC 1 cut(s) 1871
PluTI GGCGCC 1 cut(s) 1791
PpsI GAGTC 1 cut(s) 1871
Ppu21I YACGTR 1 cut(s) 2134
PscI ACATGT 1 cut(s) 2181
PshBI ATTAAT 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 919, 1416
Psp1406I AACGTT 1 cut(s) 1929
Psp6I CCWGG 3 cut(s) 576, 783, 993
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 3 cut(s) 576, 783, 993
PspN4I GGNNCC 4 cut(s) 561, 1789, 1880, 1881
PspOMI GGGCCC 1 cut(s) 1879
PspPI GGNCC 9 cut(s) 263, 734, 814, 941, 974, 1776, 1879, 1880, 2057
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 2339
PsyI GACNNNGTC 1 cut(s) 1588
PvuII CAGCTG 1 cut(s) 1457
RsaI GTAC 6 cut(s) 305, 1346, 1364, 1389, 1649, 1706
RsaNI GTAC 6 cut(s) 304, 1345, 1363, 1388, 1648, 1705
RseI CAYNNNNRTG 3 cut(s) 75, 2205, 2351
SacI GAGCTC 2 cut(s) 919, 1416
SalI GTCGAC 1 cut(s) 988
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 8 cut(s) 123, 285, 534, 867, 1632, 1794, 2093, 2241
SatI GCNGC 4 cut(s) 108, 390, 678, 1210
Sau3AI GATC 6 cut(s) 1059, 1245, 1299, 1844, 2193, 2339
Sau96I GGNCC 9 cut(s) 263, 734, 814, 941, 974, 1776, 1879, 1880, 2057
SchI GAGTC 1 cut(s) 1872
ScrFI CCNGG 6 cut(s) 578, 785, 817, 995, 1877, 2288
SduI GDGCHC 5 cut(s) 919, 1142, 1416, 1883, 2202
SfaNI GCATC 5 cut(s) 88, 394, 1171, 1440, 2327
SfcI CTRYAG 2 cut(s) 108, 1162
SfoI GGCGCC 1 cut(s) 1789
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 6 cut(s) 263, 734, 941, 974, 1776, 2057
SmiMI CAYNNNNRTG 3 cut(s) 75, 2205, 2351
SmlI CTYRAG 2 cut(s) 1415, 2092
SmoI CTYRAG 2 cut(s) 1415, 2092
SpeI ACTAGT 1 cut(s) 1102
SphI GCATGC 1 cut(s) 1576
SseBI AGGCCT 1 cut(s) 437
SspDI GGCGCC 1 cut(s) 1787
SspMI CTAG 8 cut(s) 95, 683, 1103, 1359, 1391, 1730, 1838, 1868
SstI GAGCTC 2 cut(s) 919, 1416
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 6 cut(s) 576, 783, 815, 993, 1875, 2286
StyI CCWWGG 1 cut(s) 903
TaiI ACGT 6 cut(s) 1389, 1452, 1518, 1617, 1932, 2136
TaqI TCGA 3 cut(s) 989, 1089, 1355
TatI WGTACW 1 cut(s) 1344
TfiI GAWTC 2 cut(s) 252, 706
Tru1I TTAA 8 cut(s) 123, 285, 534, 867, 1632, 1794, 2093, 2241
Tru9I TTAA 8 cut(s) 123, 285, 534, 867, 1632, 1794, 2093, 2241
TscAI CASTG 5 cut(s) 399, 451, 1044, 1778, 2049
TseFI GTSAC 4 cut(s) 689, 1045, 1652, 2306
TseI GCWGC 4 cut(s) 107, 389, 677, 1209
Tsp45I GTSAC 4 cut(s) 689, 1045, 1652, 2306
TspDTI ATGAA 5 cut(s) 59, 1003, 1021, 1189, 2015
TspGWI ACGGA 3 cut(s) 1287, 1846, 2159
TspRI CASTG 5 cut(s) 399, 451, 1044, 1778, 2049
Tth111I GACNNNGTC 1 cut(s) 1588
Vha464I CTTAAG 1 cut(s) 2092
VneI GTGCAC 1 cut(s) 2198
VpaK11BI GGWCC 6 cut(s) 263, 734, 941, 974, 1776, 2057
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 4 cut(s) 287, 1130, 1167, 1275
XbaI TCTAGA 1 cut(s) 1837
XceI RCATGY 4 cut(s) 697, 1576, 2185, 2314
XmiI GTMKAC 1 cut(s) 989
XspI CTAG 8 cut(s) 95, 683, 1103, 1359, 1391, 1730, 1838, 1868
ZraI GACGTC 1 cut(s) 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.