Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Reverse (-)

71763736 .. 71778754

Gene Structure 15019 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG514400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGGAGATGCATATCATCCGGCGGGTATACTCCTCGGCCACCTTGGCAAAAACCTCAGCCTCCCCGGGACGGATAAGAAGTTGGACGAACTAACATTTCCTGTAGACAGTCAAGAAGTAATTAGTGTTCTGGGTGAGCTTGGCTTTGGACTCTTTCTGTTCGTCATTGGAGTGAAAATGGAGGTGGAAATGATAAGAAGAATAGGACGTAAAGCGATCCTCACCGGTACTTTATGCACGTTGGTCCCTTTGGTCAGCGGTATAAGTCTTCAAAAGCTTACCGCAGGACCTGTATTTGGCATTGACAAGGAAAAAACCGACAGGCTTACGGTTGTGACTGCCATACATACACTAACTTTTTTTCCGGTTGTTGCTCTCCTTCTCGAGGAACTGAAGCTCCTCAATTCTGAAATGGGCCGGTTAAGCCTGACTGCTACCATAGTCTGTGAGCTCTTAAGTTTGTTTCTCCAGTTTGTTAACAGATCAATTTCATCCATAACTAATGAAGGACATGAGAGGGATTTAGTACACCTCGCAATAGCCTTTGGTTACGTTACTTCTGTTGCGGTAATATTTAGACCAATAATGTTATGGGTCGTTAGGAAGACCCCGGACAACAGGCCAGTCAAGAAAATCTATCTCAATGCCATAGTCCTGCTGGTGTTTGTGTCTGGAATAGGTTCACATCTATACGGCCAGACATTTCATTTTGGCCCTCTCATATTTGGTTTGGCTGTCCCAGCTGGACAACCTCTGGGATCGGCTTTGGAAGAAAAATTCCATCTCTTTGTTTTCGAAGTGTTTCTACCCCTTTTTGTGACTACATATGCAATGAGGGCTGATTTTGGGTCGATCAGATTGGACGATCCAAGAACACAGCTTACAATAATCCTCATAGTCCTCATTTTTGTGGTCAAAATATTAGCCACCATGGTCGCTCCGTTATATTTTAAGGTTCCCTTAACCGATGCATTTGTTCTTGCTCTCATTTTGAGCTGCAGAGGGATTATCCACCTTGGCACCTATACTTGGTACAGCGACACCCGGATGATAGATGATTCTGCTGATTCTGTGTATGCTGTGACACTCGCTAGCGTTGTAGTGACTTCAACTCTCGTGCCTATTATAGTGAAGCGCCTCTATGATCCTTCAAGAAAATACGCAGGCTACGAGAGAAGGGACATTATGCATTTGAAACCTAATGCAGAGCTCAAAATTCTCACCTGCATCCACAGGTCATCTAACATGCCTGCAGTGATCAATCTACTTGATGCAGCGTGTCCATCTAAAGAAAACCCCATTAATGTTTGTGTACTCCATCTCATCGAATTAATCGGCAGAACGGCTCCCATATTTATCTCCCACCAAGTGCAGGGGAAAACAGTTTCCCATGTTAATACATATTCAGCAAATGTGATCCTTTATTTCAATCAGTTTGAGAAAGAAAATCGTGGTGCTGTTAATTTGAGCATGTTCACAGCAATCTCTCCACCAAATATCATGCATGAAGACATATGCACGCTTGCAATGGACAAGCTCACATCCCTAATAATCCTCCCATTTCATCGAAAATGGTCGATTGACGGGTCCAGTATTGAGTCGGAAGACAATACTATAAGGACACTAAACTTCAGTGTACTCGAAAGAGCTCCTTGTTCCGTTGGTATCCTTGTGGATCGGGGACATTTAGGTCGGTCCACCTCTGTTGTATCCACACTATCAACATTTTCGGTTGCTGTAGTCTTCTTGGGAGGGAAAGATGACAGGGAGGCATTGACCTTTGCCAAAAGAATGGCCAGGGACTCGACGATTAGGTTAACAGTAATCCATTTAGTGGCCATGGGTAATGAATATGCTGGTGGGGAATGGGATAAAATACTGGATGCAGAGGTATTGAAGGAGTTCAAATATAACCATGTGGGTGAAGGTCTTGTAATATATTTAGAGGAAATGGTGAAAGATGGACCGCAAACAGCCTTTCTATTAAGGTCTATGGTGGCAGAGTATGATATAATCATAGTTGGGAGAAGTCATAAGTCAGAGTCACCTCAGACGGTAGGGCTTTTCGAATGGTGCGAATTTCCGGAGTTGGGGATAATAGGAGACTTGCTTGCATTACCTGATGTCGATTCTCGTCATCGCCATCAGACCTATTCACAGCATCACAACCCAGTTTCCTACTACTATGCACCATCATCGCCTTGCATGAGCCGGTTGCTCACCTTCCCCTGCAGAGCAGCCATGCTCGCTCAAAGCTCTCCTCCCAATCTCGCAGCCATGTCCAAAGCTCGCATCTGCGCTGACATCAACATCCTTCACCCTAAGGAGTTCTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

778

Amino Acids

86.26

Weight (kDa)

6.8

Isoelectric Point (pI)

40.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	8 - 378	2e-31	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	438 - 565	3.8e-12	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	576 - 705	6.2e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1232
AasI	GACNNNNNNGTC	1 cut(s)	1689
Acc36I	ACCTGC	1 cut(s)	1232
AccB1I	GGYRCC	1 cut(s)	1019
AccB7I	CCANNNNNTGG	1 cut(s)	1834
AccI	GTMKAC	2 cut(s)	28, 105
AccIII	TCCGGA	1 cut(s)	2083
AciI	CCGC	5 cut(s)	23, 258, 282, 566, 1967
AclWI	GGATC	6 cut(s)	211, 766, 860, 1139, 1411, 1683
AcoI	YGGCCR	4 cut(s)	37, 694, 1794, 1836
AcsI	RAATTY	3 cut(s)	776, 1215, 2078
AcuI	CTGAAG	2 cut(s)	413, 1615
AdeI	CACNNNGTG	1 cut(s)	1369
AfaI	GTAC	5 cut(s)	229, 528, 1034, 1314, 1638
AfiI	CCNNNNNNNGG	7 cut(s)	70, 296, 385, 1029, 1372, 1834, 2090
AflII	CTTAAG	1 cut(s)	454
AgeI	ACCGGT	1 cut(s)	224
AgsI	TTSAA	7 cut(s)	272, 1110, 1152, 1195, 1429, 1897, 1906
AjnI	CCWGG	1 cut(s)	1796
AloI	GAACNNNNNNTCC	2 cut(s)	381, 413
Alw21I	GWGCWC	3 cut(s)	453, 1212, 1651
Alw26I	GTCTC	1 cut(s)	2097
AlwI	GGATC	6 cut(s)	211, 766, 860, 1139, 1411, 1683
AlwNI	CAGNNNCTG	1 cut(s)	290
Ama87I	CYCGRG	2 cut(s)	65, 383
Aor13HI	TCCGGA	1 cut(s)	2083
AoxI	GGCC	7 cut(s)	37, 415, 620, 694, 712, 1794, 1836
ApeKI	GCWGC	4 cut(s)	996, 1274, 2237, 2273
ApoI	RAATTY	3 cut(s)	776, 1215, 2078
AseI	ATTAAT	2 cut(s)	1302, 1331
AsiGI	ACCGGT	1 cut(s)	224
Asp700I	GAANNNNTTC	3 cut(s)	679, 801, 1901
AspLEI	GCGC	2 cut(s)	1137, 2300
AspS9I	GGNCC	7 cut(s)	244, 287, 415, 713, 1587, 1695, 1964
AsuC2I	CCSGG	4 cut(s)	66, 67, 611, 1045
AsuHPI	GGTGA	8 cut(s)	146, 214, 1213, 1934, 1966, 2037, 2212, 2309
AsuII	TTCGAA	2 cut(s)	795, 2067
AsuNHI	GCTAGC	1 cut(s)	1091
AvaI	CYCGRG	2 cut(s)	65, 383
AvaII	GGWCC	5 cut(s)	244, 287, 1587, 1695, 1964
AxyI	CCTNAGG	1 cut(s)	2322
BalI	TGGCCA	2 cut(s)	1796, 1838
BanI	GGYRCC	1 cut(s)	1019
BanII	GRGCYC	3 cut(s)	453, 1212, 1651
BarI	GAAGNNNNNNTAC	2 cut(s)	789, 821
BauI	CACGAG	1 cut(s)	1115
BbsI	GAAGAC	5 cut(s)	260, 611, 1515, 1611, 1735
Bbv12I	GWGCWC	3 cut(s)	453, 1212, 1651
BbvCI	CCTCAGC	1 cut(s)	56
BbvI	GCAGC	4 cut(s)	983, 1286, 2249, 2285
BccI	CCATC	6 cut(s)	789, 1291, 1326, 1955, 2151, 2200
BceAI	ACGGC	2 cut(s)	709, 1359
BcgI	CGANNNNNNTGC	2 cut(s)	2178, 2212
BciT130I	CCWGG	1 cut(s)	1798
BciVI	GTATCC	2 cut(s)	1676, 1720
BclI	TGATCA	1 cut(s)	1257
BcnI	CCSGG	4 cut(s)	66, 67, 611, 1045
BcoDI	GTCTC	1 cut(s)	2097
BfaI	CTAG	1 cut(s)	1092
BfmI	CTRYAG	5 cut(s)	102, 997, 1251, 1737, 2230
BfoI	RGCGCY	1 cut(s)	1138
BfrI	CTTAAG	1 cut(s)	454
BfuAI	ACCTGC	1 cut(s)	1232
BfuI	GTATCC	2 cut(s)	1676, 1720
BglI	GCCNNNNNGGC	1 cut(s)	45
BisI	GCNGC	4 cut(s)	997, 1275, 2238, 2274
BlsI	GCNGC	4 cut(s)	998, 1276, 2239, 2275
Bme1390I	CCNGG	5 cut(s)	66, 67, 611, 1045, 1798
Bme18I	GGWCC	5 cut(s)	244, 287, 1587, 1695, 1964
BmeT110I	CYCGRG	2 cut(s)	65, 383
BmgT120I	GGNCC	7 cut(s)	244, 287, 415, 713, 1587, 1695, 1964
BmiI	GGNNCC	5 cut(s)	246, 957, 1021, 1347, 1588
BmrFI	CCNGG	5 cut(s)	66, 67, 611, 1045, 1798
BmrI	ACTGGG	1 cut(s)	2165
BmsI	GCATC	6 cut(s)	958, 1236, 1261, 1873, 2170, 2301
BmtI	GCTAGC	1 cut(s)	1095
BmuI	ACTGGG	1 cut(s)	2165
BpiI	GAAGAC	5 cut(s)	260, 611, 1515, 1611, 1735
BpmI	CTGGAG	1 cut(s)	452
Bpu10I	CCTNAGC	1 cut(s)	56
Bpu14I	TTCGAA	2 cut(s)	795, 2067
BpuMI	CCSGG	4 cut(s)	66, 67, 611, 1045
BsaBI	GATNNNNATC	1 cut(s)	12
BsaJI	CCNNGG	9 cut(s)	34, 43, 64, 65, 609, 930, 1015, 1797, 1839
BsaWI	WCCGGW	3 cut(s)	224, 364, 2083
BsaXI	ACNNNNNCTCC	2 cut(s)	381, 411
Bsc4I	CCNNNNNNNGG	7 cut(s)	70, 296, 385, 1029, 1372, 1834, 2090
Bse118I	RCCGGY	3 cut(s)	224, 417, 2211
Bse1I	ACTGG	5 cut(s)	469, 623, 1590, 1884, 2171
Bse21I	CCTNAGG	1 cut(s)	2322
Bse3DI	GCAATG	2 cut(s)	837, 1533
Bse8I	GATNNNNATC	1 cut(s)	12
BseAI	TCCGGA	1 cut(s)	2083
BseBI	CCWGG	1 cut(s)	1798
BseDI	CCNNGG	9 cut(s)	34, 43, 64, 65, 609, 930, 1015, 1797, 1839
BseGI	GGATG	7 cut(s)	16, 491, 1053, 1227, 1541, 1888, 2310
BseJI	GATNNNNATC	1 cut(s)	12
BseLI	CCNNNNNNNGG	7 cut(s)	70, 296, 385, 1029, 1372, 1834, 2090
BseMI	GCAATG	2 cut(s)	837, 1533
BseMII	CTCAG	2 cut(s)	70, 2063
BseNI	ACTGG	5 cut(s)	469, 623, 1590, 1884, 2171
BseRI	GAGGAG	3 cut(s)	23, 389, 2250
BseXI	GCAGC	4 cut(s)	983, 1286, 2249, 2285
BseYI	CCCAGC	1 cut(s)	739
BsgI	GTGCAG	1 cut(s)	1391
BshFI	GGCC	7 cut(s)	39, 417, 622, 696, 714, 1796, 1838
BshNI	GGYRCC	1 cut(s)	1019
BshTI	ACCGGT	1 cut(s)	224
BsiHKAI	GWGCWC	3 cut(s)	453, 1212, 1651
BsiHKCI	CYCGRG	2 cut(s)	65, 383
BsiSI	CCGG	9 cut(s)	20, 66, 225, 365, 418, 611, 1045, 2084, 2212
BslFI	GGGAC	6 cut(s)	82, 230, 722, 1193, 1695, 1814
BslI	CCNNNNNNNGG	7 cut(s)	70, 296, 385, 1029, 1372, 1834, 2090
BsmAI	GTCTC	1 cut(s)	2097
BsmFI	GGGAC	6 cut(s)	82, 230, 722, 1193, 1695, 1814
BsnI	GGCC	7 cut(s)	39, 417, 622, 696, 714, 1796, 1838
BsoBI	CYCGRG	2 cut(s)	65, 383
Bsp119I	TTCGAA	2 cut(s)	795, 2067
Bsp1286I	GDGCHC	3 cut(s)	453, 1212, 1651
Bsp13I	TCCGGA	1 cut(s)	2083
Bsp143I	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
Bsp19I	CCATGG	2 cut(s)	930, 1839
BspACI	CCGC	5 cut(s)	23, 258, 282, 566, 1967
BspANI	GGCC	7 cut(s)	39, 417, 622, 696, 714, 1796, 1838
BspCNI	CTCAG	2 cut(s)	69, 2062
BspEI	TCCGGA	1 cut(s)	2083
BspLI	GGNNCC	5 cut(s)	246, 957, 1021, 1347, 1588
BspMAI	CTGCAG	3 cut(s)	1001, 1255, 2234
BspMI	ACCTGC	1 cut(s)	1232
BspOI	GCTAGC	1 cut(s)	1095
BspPI	GGATC	6 cut(s)	211, 766, 860, 1139, 1411, 1683
BspT104I	TTCGAA	2 cut(s)	795, 2067
BspT107I	GGYRCC	1 cut(s)	1019
BspTI	CTTAAG	1 cut(s)	454
BsrDI	GCAATG	2 cut(s)	837, 1533
BsrFI	RCCGGY	3 cut(s)	224, 417, 2211
BsrI	ACTGG	5 cut(s)	469, 623, 1590, 1884, 2171
BssAI	RCCGGY	3 cut(s)	224, 417, 2211
BssECI	CCNNGG	9 cut(s)	34, 43, 64, 65, 609, 930, 1015, 1797, 1839
BssMI	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
BssNAI	GTATAC	1 cut(s)	29
BssSI	CACGAG	1 cut(s)	1115
BssT1I	CCWWGG	4 cut(s)	43, 930, 1015, 1839
Bst1107I	GTATAC	1 cut(s)	29
Bst2BI	CACGAG	1 cut(s)	1115
Bst2UI	CCWGG	1 cut(s)	1798
Bst4CI	ACNGT	5 cut(s)	110, 331, 1384, 1822, 2056
BstAFI	CTTAAG	1 cut(s)	454
BstBI	TTCGAA	2 cut(s)	795, 2067
BstC8I	GCNNGC	8 cut(s)	1093, 1165, 1251, 1520, 1524, 2112, 2247, 2290
BstDEI	CTNAG	3 cut(s)	56, 2049, 2322
BstDSI	CCRYGG	2 cut(s)	930, 1839
BstENI	CCTNNNNNAGG	1 cut(s)	383
BstF5I	GGATG	7 cut(s)	16, 491, 1053, 1227, 1541, 1888, 2310
BstH2I	RGCGCY	1 cut(s)	1138
BstHHI	GCGC	2 cut(s)	1137, 2300
BstKTI	GATC	9 cut(s)	219, 485, 761, 855, 868, 1147, 1260, 1419, 1678
BstMAI	GTCTC	1 cut(s)	2097
BstMBI	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
BstMWI	GCNNNNNNNGC	5 cut(s)	45, 423, 740, 836, 2246
BstNI	CCWGG	1 cut(s)	1798
BstNSI	RCATGY	2 cut(s)	1249, 1474
BstSCI	CCNGG	5 cut(s)	64, 65, 609, 1043, 1796
BstSFI	CTRYAG	5 cut(s)	102, 997, 1251, 1737, 2230
BstV1I	GCAGC	4 cut(s)	983, 1286, 2249, 2285
BstV2I	GAAGAC	5 cut(s)	260, 611, 1515, 1611, 1735
BstXI	CCANNNNNNTGG	1 cut(s)	1792
BstZ17I	GTATAC	1 cut(s)	29
Bsu36I	CCTNAGG	1 cut(s)	2322
BsuI	GTATCC	2 cut(s)	1676, 1720
BsuRI	GGCC	7 cut(s)	39, 417, 622, 696, 714, 1796, 1838
BtgI	CCRYGG	2 cut(s)	930, 1839
BtgZI	GCGATG	2 cut(s)	2123, 2181
BtsCI	GGATG	7 cut(s)	16, 491, 1053, 1227, 1541, 1888, 2310
BtsI	GCAGTG	1 cut(s)	1260
BtsIMutI	CAGTG	2 cut(s)	1260, 1639
BveI	ACCTGC	1 cut(s)	1232
Cac8I	GCNNGC	8 cut(s)	1093, 1165, 1251, 1520, 1524, 2112, 2247, 2290
CaiI	CAGNNNCTG	1 cut(s)	290
CfoI	GCGC	2 cut(s)	1137, 2300
Cfr10I	RCCGGY	3 cut(s)	224, 417, 2211
Cfr13I	GGNCC	7 cut(s)	244, 287, 415, 713, 1587, 1695, 1964
Cfr9I	CCCGGG	1 cut(s)	65
Csp6I	GTAC	5 cut(s)	228, 527, 1033, 1313, 1637
CspAI	ACCGGT	1 cut(s)	224
CspCI	CAANNNNNGTGG	2 cut(s)	1687, 1722
CviQI	GTAC	5 cut(s)	228, 527, 1033, 1313, 1637
DdeI	CTNAG	3 cut(s)	56, 2049, 2322
DpnI	GATC	9 cut(s)	218, 484, 760, 854, 867, 1146, 1259, 1418, 1677
DpnII	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
DraIII	CACNNNGTG	1 cut(s)	1369
DrdI	GACNNNNNNGTC	1 cut(s)	1689
DseDI	GACNNNNNNGTC	1 cut(s)	1689
EaeI	YGGCCR	4 cut(s)	37, 694, 1794, 1836
Ecl136II	GAGCTC	3 cut(s)	451, 1210, 1649
Eco130I	CCWWGG	4 cut(s)	43, 930, 1015, 1839
Eco24I	GRGCYC	3 cut(s)	453, 1212, 1651
Eco47I	GGWCC	5 cut(s)	244, 287, 1587, 1695, 1964
Eco53kI	GAGCTC	3 cut(s)	451, 1210, 1649
Eco57I	CTGAAG	2 cut(s)	413, 1615
Eco81I	CCTNAGG	1 cut(s)	2322
Eco88I	CYCGRG	2 cut(s)	65, 383
EcoICRI	GAGCTC	3 cut(s)	451, 1210, 1649
EcoNI	CCTNNNNNAGG	1 cut(s)	383
EcoO109I	RGGNCCY	1 cut(s)	287
EcoRII	CCWGG	1 cut(s)	1796
EcoT14I	CCWWGG	4 cut(s)	43, 930, 1015, 1839
EcoT22I	ATGCAT	4 cut(s)	13, 973, 1191, 1506
EcoT38I	GRGCYC	3 cut(s)	453, 1212, 1651
ErhI	CCWWGG	4 cut(s)	43, 930, 1015, 1839
FalI	AAGNNNNNCTT	4 cut(s)	944, 976, 1636, 1668
FaqI	GGGAC	6 cut(s)	82, 230, 722, 1193, 1695, 1814
FauI	CCCGC	1 cut(s)	16
FauNDI	CATATG	2 cut(s)	826, 1514
FbaI	TGATCA	1 cut(s)	1257
FblI	GTMKAC	2 cut(s)	28, 105
Fnu4HI	GCNGC	4 cut(s)	997, 1275, 2238, 2274
FokI	GGATG	7 cut(s)	3, 478, 1060, 1214, 1528, 1895, 2297
FriOI	GRGCYC	3 cut(s)	453, 1212, 1651
Fsp4HI	GCNGC	4 cut(s)	997, 1275, 2238, 2274
FspBI	CTAG	1 cut(s)	1092
GlaI	GCGC	2 cut(s)	1136, 2299
GluI	GCNGC	4 cut(s)	997, 1275, 2238, 2274
GsaI	CCCAGC	1 cut(s)	743
GsuI	CTGGAG	1 cut(s)	452
HaeII	RGCGCY	1 cut(s)	1138
HaeIII	GGCC	7 cut(s)	39, 417, 622, 696, 714, 1796, 1838
HapII	CCGG	9 cut(s)	20, 66, 225, 365, 418, 611, 1045, 2084, 2212
HhaI	GCGC	2 cut(s)	1137, 2300
Hin6I	GCGC	2 cut(s)	1135, 2298
HinP1I	GCGC	2 cut(s)	1135, 2298
HincII	GTYRAC	2 cut(s)	478, 1818
HindII	GTYRAC	2 cut(s)	478, 1818
HindIII	AAGCTT	1 cut(s)	275
HinfI	GANTC	7 cut(s)	150, 1058, 1067, 1598, 1802, 2042, 2129
HpaI	GTTAAC	2 cut(s)	478, 1818
HpaII	CCGG	9 cut(s)	20, 66, 225, 365, 418, 611, 1045, 2084, 2212
HphI	GGTGA	8 cut(s)	146, 214, 1213, 1934, 1966, 2037, 2212, 2309
Hpy188I	TCNGA	6 cut(s)	409, 857, 1603, 2041, 2052, 2148
Hpy188III	TCNNGA	6 cut(s)	113, 383, 628, 672, 1152, 2084
Hpy99I	CGWCG	1 cut(s)	1810
HpyAV	CCTTC	8 cut(s)	389, 500, 1158, 1170, 1891, 1919, 2233, 2324
HpyCH4III	ACNGT	5 cut(s)	110, 331, 1384, 1822, 2056
HpyCH4IV	ACGT	3 cut(s)	208, 239, 552
HpyF10VI	GCNNNNNNNGC	5 cut(s)	45, 423, 740, 836, 2246
HpyF3I	CTNAG	3 cut(s)	56, 2049, 2322
HpySE526I	ACGT	3 cut(s)	208, 239, 552
HspAI	GCGC	2 cut(s)	1135, 2298
Kpn2I	TCCGGA	1 cut(s)	2083
Ksp22I	TGATCA	1 cut(s)	1257
KspAI	GTTAAC	2 cut(s)	478, 1818
Kzo9I	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
LmnI	GCTCC	4 cut(s)	402, 943, 1351, 1654
Lsp1109I	GCAGC	4 cut(s)	983, 1286, 2249, 2285
LweI	GCATC	6 cut(s)	958, 1236, 1261, 1873, 2170, 2301
MaeI	CTAG	1 cut(s)	1092
MaeII	ACGT	3 cut(s)	208, 239, 552
MaeIII	GTNAC	7 cut(s)	334, 548, 553, 817, 1081, 1102, 2043
MalI	GATC	9 cut(s)	218, 484, 760, 854, 867, 1146, 1259, 1418, 1677
MboI	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
MboII	GAAGA	7 cut(s)	210, 260, 616, 782, 1520, 1616, 1735
MhlI	GDGCHC	3 cut(s)	453, 1212, 1651
MlsI	TGGCCA	2 cut(s)	1796, 1838
MluCI	AATT	8 cut(s)	120, 403, 486, 776, 1215, 1328, 1462, 2078
MluNI	TGGCCA	2 cut(s)	1796, 1838
MlyI	GAGTC	4 cut(s)	144, 1607, 1796, 2051
MmeI	TCCRAC	2 cut(s)	63, 1581
Mox20I	TGGCCA	2 cut(s)	1796, 1838
Mph1103I	ATGCAT	4 cut(s)	13, 973, 1191, 1506
MroI	TCCGGA	1 cut(s)	2083
MroXI	GAANNNNTTC	3 cut(s)	679, 801, 1901
MscI	TGGCCA	2 cut(s)	1796, 1838
MslI	CAYNNNNRTG	4 cut(s)	170, 908, 1046, 1920
Msp20I	TGGCCA	2 cut(s)	1796, 1838
MspA1I	CMGCKG	2 cut(s)	258, 743
MspCI	CTTAAG	1 cut(s)	454
MspI	CCGG	9 cut(s)	20, 66, 225, 365, 418, 611, 1045, 2084, 2212
MspR9I	CCNGG	5 cut(s)	66, 67, 611, 1045, 1798
MvaI	CCWGG	1 cut(s)	1798
MwoI	GCNNNNNNNGC	5 cut(s)	45, 423, 740, 836, 2246
NciI	CCSGG	4 cut(s)	66, 67, 611, 1045
NcoI	CCATGG	2 cut(s)	930, 1839
NdeI	CATATG	2 cut(s)	826, 1514
NdeII	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
NheI	GCTAGC	1 cut(s)	1091
NlaIV	GGNNCC	5 cut(s)	246, 957, 1021, 1347, 1588
NmeAIII	GCCGAG	1 cut(s)	15
NmuCI	GTSAC	5 cut(s)	334, 817, 1081, 1102, 2043
NsiI	ATGCAT	4 cut(s)	13, 973, 1191, 1506
NspI	RCATGY	2 cut(s)	1249, 1474
NspV	TTCGAA	2 cut(s)	795, 2067
PaeR7I	CTCGAG	1 cut(s)	383
PaqCI	CACCTGC	1 cut(s)	1232
PcsI	WCGNNNNNNNCGW	2 cut(s)	1167, 2073
PdmI	GAANNNNTTC	3 cut(s)	679, 801, 1901
PfeI	GAWTC	3 cut(s)	1058, 1067, 2129
PflMI	CCANNNNNTGG	1 cut(s)	1834
PinAI	ACCGGT	1 cut(s)	224
PkrI	GCNGC	4 cut(s)	998, 1276, 2239, 2275
PleI	GAGTC	4 cut(s)	144, 1606, 1796, 2050
PpsI	GAGTC	4 cut(s)	144, 1606, 1796, 2050
PpuMI	RGGWCCY	1 cut(s)	287
PshBI	ATTAAT	2 cut(s)	1302, 1331
Psp124BI	GAGCTC	3 cut(s)	453, 1212, 1651
Psp5II	RGGWCCY	1 cut(s)	287
Psp6I	CCWGG	1 cut(s)	1796
PspFI	CCCAGC	1 cut(s)	739
PspGI	CCWGG	1 cut(s)	1796
PspN4I	GGNNCC	5 cut(s)	246, 957, 1021, 1347, 1588
PspPI	GGNCC	7 cut(s)	244, 287, 415, 713, 1587, 1695, 1964
PspPPI	RGGWCCY	1 cut(s)	287
PsrI	GAACNNNNNNTAC	4 cut(s)	111, 143, 865, 897
PstI	CTGCAG	3 cut(s)	1001, 1255, 2234
PstNI	CAGNNNCTG	1 cut(s)	290
PvuII	CAGCTG	1 cut(s)	743
RsaI	GTAC	5 cut(s)	229, 528, 1034, 1314, 1638
RsaNI	GTAC	5 cut(s)	228, 527, 1033, 1313, 1637
RseI	CAYNNNNRTG	4 cut(s)	170, 908, 1046, 1920
SacI	GAGCTC	3 cut(s)	453, 1212, 1651
SatI	GCNGC	4 cut(s)	997, 1275, 2238, 2274
Sau3AI	GATC	9 cut(s)	216, 482, 758, 852, 865, 1144, 1257, 1416, 1675
Sau96I	GGNCC	7 cut(s)	244, 287, 415, 713, 1587, 1695, 1964
SchI	GAGTC	4 cut(s)	144, 1607, 1796, 2051
ScrFI	CCNGG	5 cut(s)	66, 67, 611, 1045, 1798
SduI	GDGCHC	3 cut(s)	453, 1212, 1651
SfaNI	GCATC	6 cut(s)	958, 1236, 1261, 1873, 2170, 2301
SfcI	CTRYAG	5 cut(s)	102, 997, 1251, 1737, 2230
Sfr274I	CTCGAG	1 cut(s)	383
SfuI	TTCGAA	2 cut(s)	795, 2067
SinI	GGWCC	5 cut(s)	244, 287, 1587, 1695, 1964
SlaI	CTCGAG	1 cut(s)	383
SmaI	CCCGGG	1 cut(s)	67
SmiMI	CAYNNNNRTG	4 cut(s)	170, 908, 1046, 1920
SmlI	CTYRAG	2 cut(s)	383, 454
SmoI	CTYRAG	2 cut(s)	383, 454
Sse9I	AATT	8 cut(s)	120, 403, 486, 776, 1215, 1328, 1462, 2078
SsiI	CCGC	5 cut(s)	23, 258, 282, 566, 1967
SspI	AATATT	2 cut(s)	573, 921
SspMI	CTAG	1 cut(s)	1092
SstI	GAGCTC	3 cut(s)	453, 1212, 1651
StyD4I	CCNGG	5 cut(s)	64, 65, 609, 1043, 1796
StyI	CCWWGG	4 cut(s)	43, 930, 1015, 1839
TaaI	ACNGT	5 cut(s)	110, 331, 1384, 1822, 2056
TaiI	ACGT	3 cut(s)	211, 242, 555
TaqII	GACCGA	1 cut(s)	1683
TasI	AATT	8 cut(s)	120, 403, 486, 776, 1215, 1328, 1462, 2078
TatI	WGTACW	3 cut(s)	526, 1312, 1636
TfiI	GAWTC	3 cut(s)	1058, 1067, 2129
TscAI	CASTG	2 cut(s)	1260, 1639
TseFI	GTSAC	5 cut(s)	334, 817, 1081, 1102, 2043
TseI	GCWGC	4 cut(s)	996, 1274, 2237, 2273
Tsp45I	GTSAC	5 cut(s)	334, 817, 1081, 1102, 2043
TspDTI	ATGAA	6 cut(s)	480, 519, 695, 1521, 1553, 1863
TspGWI	ACGGA	3 cut(s)	86, 930, 1648
TspMI	CCCGGG	1 cut(s)	65
TspRI	CASTG	2 cut(s)	1260, 1639
Van91I	CCANNNNNTGG	1 cut(s)	1834
Vha464I	CTTAAG	1 cut(s)	454
VpaK11BI	GGWCC	5 cut(s)	244, 287, 1587, 1695, 1964
VspI	ATTAAT	2 cut(s)	1302, 1331
XagI	CCTNNNNNAGG	1 cut(s)	383
XapI	RAATTY	3 cut(s)	776, 1215, 2078
XceI	RCATGY	2 cut(s)	1249, 1474
XcmI	CCANNNNNNNNNTGG	2 cut(s)	588, 655
XhoI	CTCGAG	1 cut(s)	383
XmaI	CCCGGG	1 cut(s)	65
XmiI	GTMKAC	2 cut(s)	28, 105
XmnI	GAANNNNTTC	3 cut(s)	679, 801, 1901
XspI	CTAG	1 cut(s)	1092
Zsp2I	ATGCAT	4 cut(s)	13, 973, 1191, 1506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.