Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Reverse (-)

80699071 .. 80702536

Gene Structure 3466 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG470700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGCTGGAAATGCAGATGGCCATCATTTTCATCATCACCCAAGTCCTCCATTTAATTTTGAAGAAGTTTCATATCTCTAAGTTCAACAGCCAGCTTCTTGTGGGTATACTCCTCGGCCACCTTGGCAAAAACCTCAGCCTCCCCGGGACGGATAAGAAGTTGGACGAACTAACATTTCCTGTAGACAGTCAAGAAGTAATTAGTGTTCTGGGTGAGCTTGGCTTTGGACTCTTTCTGTTCGTCATTGGAGTGAAAATGGAGGTGGAAATGATAAGAAGAATAGGACGTAAAGCGATCCTCACCGGTACTTTATGCACGTTGGTCCCTTTGGTCAGTGGTATAAGTCTTCAAAAGCTTACCGCAGGACCTGTATTTGGCATTGACAAGGAAAAAACCGACAGGCTTACGGTTGTGACTGCCATACATACACTAACTTTTTTTCCGGTTGTTTCTCTCCTTCTCGAGGAACTGAAGCTCCTCAATTCTGAAATGGGCCGGTTAAGCCTGACTGCTACCATAGTCTGTGAGCTCTTAAGTTTGTTTCTCCAGTTTGTTAACAGATCAATTTCATCCATAACTAATGAAGGACATGAGAGGGATTTAGTACACCTCGCAATAGCCTTTGGTTACGTTACTTCTGTTGCGGTAATATTTAGACCAATAATGTTATGGGTTGTTAGGAAGACCCCGGACAACAGGCCAGTCAAGAAAATCTATCTCAATGCCATAGTCCTGCTGGTGTTTGTGTCTGGAATAGGTTCACATCTATACGGCCAGACATTTCATTTTGGCCCTCTCATATTTGGTTTGGCTGTCCCAGCTGGACAACCTCTGGGATCGGCTTTGGAAGAAAAATTCCATCTCTTTGTTTTCGAAGTGTTTCTACCCCTTTTTGTGACTACATATGCAATGAGGGCTGATTTTGGGTCGATCAGATTGGACGATCCAAGAACACAGCTTACTATAATCCTCATAGTCCTCATTTTTGTGGTCAAAATATTAGCCACCATGGTCGCTCCGTTATATTTTAAGGTTCCCTTAACCGATGCATTTGTTCTTGCTCTCATTTTGAGCTGCAGAGGGATTATCCACCTTGGCACCTATACTTGGTACAGCGACACCCGGATGATAGATGATTCTGCTGATTCTGTGTATGCTGTGACACTCGCTAGCGTTGTAGTGACTTCAACTCTCGTGCCTATTATAGTGAAGCGCCTCTATGATCCTTCAAGAAAATACGCAGGCTACGAGAGAAGGGACATTATGCATTTGAAACCTAATGCAGAGCTCAAAATTCTCACCTGCATCCACAGGTCATCTAACATGCCTGCAGTGATCAATCTACTTGATGCAGCGTGTCCATCTAAAAAAAACCCCATTAATGTTTGTGTACTCCATCTCATCGAATTAATCGGCAGAACGGCTCCCATATTTATCTCCCACCAAGTGCAGGGGAAAACAGTTTCCCATGTTAATACATATTCAGCAAATGTGATTCTTTATTTCGATCAGTTTGAGAAAGAAAATCGTGGTGCTGTTAATTTGAGCATATTCACAGCAATCTCTCCACCAAATATCATGCATGAAGACATATGCACGCTTGCAATGGACAAGCTCACATCCCTAATAATCCTCCCATTTCATCGAAAATGGTCGATTGACGGGTCCAGTATTGAGTCAGAAGACAATACCATAAGGACACTAAACTTCAGTGTACTCGAAAGAGCTCCTTGTTCCGTTGGTATCCTTGTGGATCGGGGACATTTAGGTCGGTCCACCTCTGTTGTATCCACACTATCAACATTTTCGGTTGCTGTAGTCTTCTTGGGAGGGAAAGATGACAGGGAGGCATTGACCTTTGCCAAAAGAATGGCCAGGGACTCGACGATTAGGTTAACAGTAATCCATTTAGTGGCCATGGGTAATGAATATGCTGGTGGGGAATGGGATAAAATACTGGATGCAGAGGTATTGAAGGAGTTCAAATATAACCATGTGGGTGAAGGTCTTGTAATATATTTAGAGGAAATGGTGAAAGATGGACCGCAAACAGCCTTTCTATTAAGGTCTATGGTGGAAGAGTATGATATAATCATAGTTGGGAGAAGTCATAAGTTAGGGTCACCTCAGACAGTAGGGCTTTCCGAATGGTGCGAATTTCCGGAGTTGGGGATAATAGGAGACTTGCTTGCATCACCTGATGTCGATTGTAAGGCCTCCATTCTTGTGATGCAACAACAAGAGTTGAGCAGATGCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

753

Amino Acids

83.63

Weight (kDa)

6.88

Isoelectric Point (pI)

35.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	15 - 404	6.1e-32	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	463 - 591	3e-12	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	602 - 746	1.8e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1310
AasI	GACNNNNNNGTC	1 cut(s)	1767
Acc36I	ACCTGC	1 cut(s)	1310
AccB1I	GGYRCC	1 cut(s)	1097
AccB7I	CCANNNNNTGG	1 cut(s)	1912
AccI	GTMKAC	2 cut(s)	106, 183
AccIII	TCCGGA	1 cut(s)	2161
AciI	CCGC	3 cut(s)	360, 644, 2045
AclWI	GGATC	5 cut(s)	289, 844, 938, 1217, 1761
AcoI	YGGCCR	5 cut(s)	18, 115, 772, 1872, 1914
AcsI	RAATTY	3 cut(s)	854, 1293, 2156
AcuI	CTGAAG	2 cut(s)	491, 1693
AdeI	CACNNNGTG	1 cut(s)	1447
AfaI	GTAC	5 cut(s)	307, 606, 1112, 1392, 1716
AfiI	CCNNNNNNNGG	7 cut(s)	148, 374, 463, 1107, 1450, 1912, 2168
AflII	CTTAAG	1 cut(s)	532
AgeI	ACCGGT	1 cut(s)	302
AgsI	TTSAA	8 cut(s)	61, 85, 350, 1188, 1230, 1273, 1975, 1984
AjnI	CCWGG	1 cut(s)	1874
AloI	GAACNNNNNNTCC	2 cut(s)	459, 491
Alw21I	GWGCWC	3 cut(s)	531, 1290, 1729
Alw26I	GTCTC	1 cut(s)	2175
AlwI	GGATC	5 cut(s)	289, 844, 938, 1217, 1761
AlwNI	CAGNNNCTG	1 cut(s)	368
Ama87I	CYCGRG	2 cut(s)	143, 461
Aor13HI	TCCGGA	1 cut(s)	2161
AoxI	GGCC	9 cut(s)	18, 115, 493, 698, 772, 790, 1872, 1914, 2214
ApeKI	GCWGC	2 cut(s)	1074, 1352
ApoI	RAATTY	3 cut(s)	854, 1293, 2156
AseI	ATTAAT	2 cut(s)	1380, 1409
AsiGI	ACCGGT	1 cut(s)	302
Asp700I	GAANNNNTTC	3 cut(s)	757, 879, 1979
AspLEI	GCGC	1 cut(s)	1215
AspS9I	GGNCC	7 cut(s)	322, 365, 493, 791, 1665, 1773, 2042
AsuC2I	CCSGG	4 cut(s)	144, 145, 689, 1123
AsuHPI	GGTGA	8 cut(s)	28, 224, 292, 1291, 2012, 2044, 2115, 2187
AsuII	TTCGAA	1 cut(s)	873
AsuNHI	GCTAGC	1 cut(s)	1169
AvaI	CYCGRG	2 cut(s)	143, 461
AvaII	GGWCC	5 cut(s)	322, 365, 1665, 1773, 2042
BalI	TGGCCA	3 cut(s)	20, 1874, 1916
BanI	GGYRCC	1 cut(s)	1097
BanII	GRGCYC	3 cut(s)	531, 1290, 1729
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BauI	CACGAG	1 cut(s)	1193
BbsI	GAAGAC	5 cut(s)	338, 689, 1593, 1689, 1813
Bbv12I	GWGCWC	3 cut(s)	531, 1290, 1729
BbvCI	CCTCAGC	1 cut(s)	134
BbvI	GCAGC	2 cut(s)	1061, 1364
BccI	CCATC	6 cut(s)	10, 29, 867, 1369, 1404, 2033
BceAI	ACGGC	2 cut(s)	787, 1437
BciT130I	CCWGG	1 cut(s)	1876
BciVI	GTATCC	2 cut(s)	1754, 1798
BclI	TGATCA	1 cut(s)	1335
BcnI	CCSGG	4 cut(s)	144, 145, 689, 1123
BcoDI	GTCTC	1 cut(s)	2175
BfaI	CTAG	1 cut(s)	1170
BfmI	CTRYAG	4 cut(s)	180, 1075, 1329, 1815
BfoI	RGCGCY	1 cut(s)	1216
BfrI	CTTAAG	1 cut(s)	532
BfuAI	ACCTGC	1 cut(s)	1310
BfuI	GTATCC	2 cut(s)	1754, 1798
BglI	GCCNNNNNGGC	1 cut(s)	123
BisI	GCNGC	2 cut(s)	1075, 1353
BlsI	GCNGC	2 cut(s)	1076, 1354
Bme1390I	CCNGG	5 cut(s)	144, 145, 689, 1123, 1876
Bme18I	GGWCC	5 cut(s)	322, 365, 1665, 1773, 2042
BmeT110I	CYCGRG	2 cut(s)	143, 461
BmgT120I	GGNCC	7 cut(s)	322, 365, 493, 791, 1665, 1773, 2042
BmiI	GGNNCC	5 cut(s)	324, 1035, 1099, 1425, 1666
BmrFI	CCNGG	5 cut(s)	144, 145, 689, 1123, 1876
BmsI	GCATC	7 cut(s)	1036, 1314, 1339, 1951, 2201, 2220, 2243
BmtI	GCTAGC	1 cut(s)	1173
BpiI	GAAGAC	5 cut(s)	338, 689, 1593, 1689, 1813
BpmI	CTGGAG	1 cut(s)	530
Bpu10I	CCTNAGC	1 cut(s)	134
Bpu14I	TTCGAA	1 cut(s)	873
BpuMI	CCSGG	4 cut(s)	144, 145, 689, 1123
BsaBI	GATNNNNATC	1 cut(s)	20
BsaJI	CCNNGG	9 cut(s)	112, 121, 142, 143, 687, 1008, 1093, 1875, 1917
BsaWI	WCCGGW	3 cut(s)	302, 442, 2161
BsaXI	ACNNNNNCTCC	2 cut(s)	459, 489
Bsc4I	CCNNNNNNNGG	7 cut(s)	148, 374, 463, 1107, 1450, 1912, 2168
Bse118I	RCCGGY	2 cut(s)	302, 495
Bse1I	ACTGG	4 cut(s)	547, 701, 1668, 1962
Bse3DI	GCAATG	2 cut(s)	915, 1611
Bse8I	GATNNNNATC	1 cut(s)	20
BseAI	TCCGGA	1 cut(s)	2161
BseBI	CCWGG	1 cut(s)	1876
BseDI	CCNNGG	9 cut(s)	112, 121, 142, 143, 687, 1008, 1093, 1875, 1917
BseGI	GGATG	5 cut(s)	569, 1131, 1305, 1619, 1966
BseJI	GATNNNNATC	1 cut(s)	20
BseLI	CCNNNNNNNGG	7 cut(s)	148, 374, 463, 1107, 1450, 1912, 2168
BseMI	GCAATG	2 cut(s)	915, 1611
BseMII	CTCAG	2 cut(s)	148, 2141
BseNI	ACTGG	4 cut(s)	547, 701, 1668, 1962
BseRI	GAGGAG	2 cut(s)	101, 467
BseXI	GCAGC	2 cut(s)	1061, 1364
BseYI	CCCAGC	1 cut(s)	817
BsgI	GTGCAG	1 cut(s)	1469
BshFI	GGCC	9 cut(s)	20, 117, 495, 700, 774, 792, 1874, 1916, 2216
BshNI	GGYRCC	1 cut(s)	1097
BshTI	ACCGGT	1 cut(s)	302
BsiHKAI	GWGCWC	3 cut(s)	531, 1290, 1729
BsiHKCI	CYCGRG	2 cut(s)	143, 461
BsiSI	CCGG	7 cut(s)	144, 303, 443, 496, 689, 1123, 2162
BslFI	GGGAC	6 cut(s)	160, 308, 800, 1271, 1773, 1892
BslI	CCNNNNNNNGG	7 cut(s)	148, 374, 463, 1107, 1450, 1912, 2168
BsmAI	GTCTC	1 cut(s)	2175
BsmFI	GGGAC	6 cut(s)	160, 308, 800, 1271, 1773, 1892
BsnI	GGCC	9 cut(s)	20, 117, 495, 700, 774, 792, 1874, 1916, 2216
BsoBI	CYCGRG	2 cut(s)	143, 461
Bsp119I	TTCGAA	1 cut(s)	873
Bsp1286I	GDGCHC	3 cut(s)	531, 1290, 1729
Bsp13I	TCCGGA	1 cut(s)	2161
Bsp143I	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
Bsp19I	CCATGG	2 cut(s)	1008, 1917
BspACI	CCGC	3 cut(s)	360, 644, 2045
BspANI	GGCC	9 cut(s)	20, 117, 495, 700, 774, 792, 1874, 1916, 2216
BspCNI	CTCAG	2 cut(s)	147, 2140
BspEI	TCCGGA	1 cut(s)	2161
BspLI	GGNNCC	5 cut(s)	324, 1035, 1099, 1425, 1666
BspMAI	CTGCAG	2 cut(s)	1079, 1333
BspMI	ACCTGC	1 cut(s)	1310
BspOI	GCTAGC	1 cut(s)	1173
BspPI	GGATC	5 cut(s)	289, 844, 938, 1217, 1761
BspT104I	TTCGAA	1 cut(s)	873
BspT107I	GGYRCC	1 cut(s)	1097
BspTI	CTTAAG	1 cut(s)	532
BsrDI	GCAATG	2 cut(s)	915, 1611
BsrFI	RCCGGY	2 cut(s)	302, 495
BsrI	ACTGG	4 cut(s)	547, 701, 1668, 1962
BssAI	RCCGGY	2 cut(s)	302, 495
BssECI	CCNNGG	9 cut(s)	112, 121, 142, 143, 687, 1008, 1093, 1875, 1917
BssMI	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
BssNAI	GTATAC	1 cut(s)	107
BssSI	CACGAG	1 cut(s)	1193
BssT1I	CCWWGG	4 cut(s)	121, 1008, 1093, 1917
Bst1107I	GTATAC	1 cut(s)	107
Bst2BI	CACGAG	1 cut(s)	1193
Bst2UI	CCWGG	1 cut(s)	1876
Bst4CI	ACNGT	5 cut(s)	188, 409, 1462, 1900, 2134
Bst6I	CTCTTC	1 cut(s)	2073
BstAFI	CTTAAG	1 cut(s)	532
BstBI	TTCGAA	1 cut(s)	873
BstC8I	GCNNGC	7 cut(s)	92, 1171, 1243, 1329, 1598, 1602, 2190
BstDEI	CTNAG	3 cut(s)	78, 134, 2127
BstDSI	CCRYGG	2 cut(s)	1008, 1917
BstEII	GGTNACC	1 cut(s)	2121
BstENI	CCTNNNNNAGG	1 cut(s)	461
BstF5I	GGATG	5 cut(s)	569, 1131, 1305, 1619, 1966
BstH2I	RGCGCY	1 cut(s)	1216
BstHHI	GCGC	1 cut(s)	1215
BstKTI	GATC	9 cut(s)	297, 563, 839, 933, 946, 1225, 1338, 1510, 1756
BstMAI	GTCTC	1 cut(s)	2175
BstMBI	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
BstMWI	GCNNNNNNNGC	5 cut(s)	10, 123, 501, 818, 914
BstNI	CCWGG	1 cut(s)	1876
BstNSI	RCATGY	1 cut(s)	1327
BstPI	GGTNACC	1 cut(s)	2121
BstSCI	CCNGG	5 cut(s)	142, 143, 687, 1121, 1874
BstSFI	CTRYAG	4 cut(s)	180, 1075, 1329, 1815
BstV1I	GCAGC	2 cut(s)	1061, 1364
BstV2I	GAAGAC	5 cut(s)	338, 689, 1593, 1689, 1813
BstXI	CCANNNNNNTGG	1 cut(s)	1870
BstZ17I	GTATAC	1 cut(s)	107
BsuI	GTATCC	2 cut(s)	1754, 1798
BsuRI	GGCC	9 cut(s)	20, 117, 495, 700, 774, 792, 1874, 1916, 2216
BtgI	CCRYGG	2 cut(s)	1008, 1917
BtsCI	GGATG	5 cut(s)	569, 1131, 1305, 1619, 1966
BtsI	GCAGTG	1 cut(s)	1338
BtsIMutI	CAGTG	3 cut(s)	340, 1338, 1717
BveI	ACCTGC	1 cut(s)	1310
Cac8I	GCNNGC	7 cut(s)	92, 1171, 1243, 1329, 1598, 1602, 2190
CaiI	CAGNNNCTG	1 cut(s)	368
CfoI	GCGC	1 cut(s)	1215
Cfr10I	RCCGGY	2 cut(s)	302, 495
Cfr13I	GGNCC	7 cut(s)	322, 365, 493, 791, 1665, 1773, 2042
Cfr9I	CCCGGG	1 cut(s)	143
Csp6I	GTAC	5 cut(s)	306, 605, 1111, 1391, 1715
CspAI	ACCGGT	1 cut(s)	302
CspCI	CAANNNNNGTGG	2 cut(s)	1765, 1800
CviAII	CATG	8 cut(s)	590, 1009, 1324, 1469, 1579, 1583, 1918, 1994
CviQI	GTAC	5 cut(s)	306, 605, 1111, 1391, 1715
DdeI	CTNAG	3 cut(s)	78, 134, 2127
DpnI	GATC	9 cut(s)	296, 562, 838, 932, 945, 1224, 1337, 1509, 1755
DpnII	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
DraIII	CACNNNGTG	1 cut(s)	1447
DrdI	GACNNNNNNGTC	1 cut(s)	1767
DseDI	GACNNNNNNGTC	1 cut(s)	1767
EaeI	YGGCCR	5 cut(s)	18, 115, 772, 1872, 1914
Eam1104I	CTCTTC	1 cut(s)	2073
EarI	CTCTTC	1 cut(s)	2073
Ecl136II	GAGCTC	3 cut(s)	529, 1288, 1727
Eco130I	CCWWGG	4 cut(s)	121, 1008, 1093, 1917
Eco147I	AGGCCT	1 cut(s)	2216
Eco24I	GRGCYC	3 cut(s)	531, 1290, 1729
Eco47I	GGWCC	5 cut(s)	322, 365, 1665, 1773, 2042
Eco53kI	GAGCTC	3 cut(s)	529, 1288, 1727
Eco57I	CTGAAG	2 cut(s)	491, 1693
Eco88I	CYCGRG	2 cut(s)	143, 461
Eco91I	GGTNACC	1 cut(s)	2121
EcoICRI	GAGCTC	3 cut(s)	529, 1288, 1727
EcoNI	CCTNNNNNAGG	1 cut(s)	461
EcoO109I	RGGNCCY	1 cut(s)	365
EcoO65I	GGTNACC	1 cut(s)	2121
EcoRII	CCWGG	1 cut(s)	1874
EcoT14I	CCWWGG	4 cut(s)	121, 1008, 1093, 1917
EcoT22I	ATGCAT	3 cut(s)	1051, 1269, 1584
EcoT38I	GRGCYC	3 cut(s)	531, 1290, 1729
ErhI	CCWWGG	4 cut(s)	121, 1008, 1093, 1917
FaeI	CATG	8 cut(s)	593, 1012, 1327, 1472, 1582, 1586, 1921, 1997
FalI	AAGNNNNNCTT	4 cut(s)	1022, 1054, 1714, 1746
FaqI	GGGAC	6 cut(s)	160, 308, 800, 1271, 1773, 1892
FatI	CATG	8 cut(s)	589, 1008, 1323, 1468, 1578, 1582, 1917, 1993
FauNDI	CATATG	2 cut(s)	904, 1592
FbaI	TGATCA	1 cut(s)	1335
FblI	GTMKAC	2 cut(s)	106, 183
Fnu4HI	GCNGC	2 cut(s)	1075, 1353
FokI	GGATG	5 cut(s)	556, 1138, 1292, 1606, 1973
FriOI	GRGCYC	3 cut(s)	531, 1290, 1729
Fsp4HI	GCNGC	2 cut(s)	1075, 1353
FspBI	CTAG	1 cut(s)	1170
GlaI	GCGC	1 cut(s)	1214
GluI	GCNGC	2 cut(s)	1075, 1353
GsaI	CCCAGC	1 cut(s)	821
GsuI	CTGGAG	1 cut(s)	530
HaeII	RGCGCY	1 cut(s)	1216
HaeIII	GGCC	9 cut(s)	20, 117, 495, 700, 774, 792, 1874, 1916, 2216
HapII	CCGG	7 cut(s)	144, 303, 443, 496, 689, 1123, 2162
HhaI	GCGC	1 cut(s)	1215
Hin1II	CATG	8 cut(s)	593, 1012, 1327, 1472, 1582, 1586, 1921, 1997
Hin6I	GCGC	1 cut(s)	1213
HinP1I	GCGC	1 cut(s)	1213
HincII	GTYRAC	2 cut(s)	556, 1896
HindII	GTYRAC	2 cut(s)	556, 1896
HindIII	AAGCTT	1 cut(s)	353
HinfI	GANTC	6 cut(s)	228, 1136, 1145, 1495, 1676, 1880
HpaI	GTTAAC	2 cut(s)	556, 1896
HpaII	CCGG	7 cut(s)	144, 303, 443, 496, 689, 1123, 2162
HphI	GGTGA	8 cut(s)	28, 224, 292, 1291, 2012, 2044, 2115, 2187
Hpy166II	GTNNAC	9 cut(s)	107, 184, 556, 607, 761, 1391, 1715, 1776, 1896
Hpy188I	TCNGA	5 cut(s)	487, 935, 1681, 2130, 2146
Hpy188III	TCNNGA	6 cut(s)	191, 461, 706, 750, 1230, 2162
Hpy8I	GTNNAC	9 cut(s)	107, 184, 556, 607, 761, 1391, 1715, 1776, 1896
Hpy99I	CGWCG	1 cut(s)	1888
HpyAV	CCTTC	6 cut(s)	467, 578, 1236, 1248, 1969, 1997
HpyCH4III	ACNGT	5 cut(s)	188, 409, 1462, 1900, 2134
HpyCH4IV	ACGT	3 cut(s)	286, 317, 630
HpyF10VI	GCNNNNNNNGC	5 cut(s)	10, 123, 501, 818, 914
HpyF3I	CTNAG	3 cut(s)	78, 134, 2127
HpySE526I	ACGT	3 cut(s)	286, 317, 630
Hsp92II	CATG	8 cut(s)	593, 1012, 1327, 1472, 1582, 1586, 1921, 1997
HspAI	GCGC	1 cut(s)	1213
Kpn2I	TCCGGA	1 cut(s)	2161
Ksp22I	TGATCA	1 cut(s)	1335
KspAI	GTTAAC	2 cut(s)	556, 1896
Kzo9I	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
LmnI	GCTCC	4 cut(s)	480, 1021, 1429, 1732
Lsp1109I	GCAGC	2 cut(s)	1061, 1364
LweI	GCATC	7 cut(s)	1036, 1314, 1339, 1951, 2201, 2220, 2243
MaeI	CTAG	1 cut(s)	1170
MaeII	ACGT	3 cut(s)	286, 317, 630
MaeIII	GTNAC	7 cut(s)	412, 626, 631, 895, 1159, 1180, 2121
MalI	GATC	9 cut(s)	296, 562, 838, 932, 945, 1224, 1337, 1509, 1755
MboI	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
MboII	GAAGA	9 cut(s)	73, 288, 338, 694, 860, 1598, 1694, 1813, 2090
MhlI	GDGCHC	3 cut(s)	531, 1290, 1729
MlsI	TGGCCA	3 cut(s)	20, 1874, 1916
MluCI	AATT	9 cut(s)	54, 198, 481, 564, 854, 1293, 1406, 1540, 2156
MluNI	TGGCCA	3 cut(s)	20, 1874, 1916
MlyI	GAGTC	3 cut(s)	222, 1685, 1874
MmeI	TCCRAC	1 cut(s)	141
Mox20I	TGGCCA	3 cut(s)	20, 1874, 1916
Mph1103I	ATGCAT	3 cut(s)	1051, 1269, 1584
MroI	TCCGGA	1 cut(s)	2161
MroXI	GAANNNNTTC	3 cut(s)	757, 879, 1979
MscI	TGGCCA	3 cut(s)	20, 1874, 1916
MslI	CAYNNNNRTG	5 cut(s)	248, 986, 1124, 1998, 2225
Msp20I	TGGCCA	3 cut(s)	20, 1874, 1916
MspA1I	CMGCKG	1 cut(s)	821
MspCI	CTTAAG	1 cut(s)	532
MspI	CCGG	7 cut(s)	144, 303, 443, 496, 689, 1123, 2162
MspR9I	CCNGG	5 cut(s)	144, 145, 689, 1123, 1876
MvaI	CCWGG	1 cut(s)	1876
MwoI	GCNNNNNNNGC	5 cut(s)	10, 123, 501, 818, 914
NciI	CCSGG	4 cut(s)	144, 145, 689, 1123
NcoI	CCATGG	2 cut(s)	1008, 1917
NdeI	CATATG	2 cut(s)	904, 1592
NdeII	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
NheI	GCTAGC	1 cut(s)	1169
NlaIII	CATG	8 cut(s)	593, 1012, 1327, 1472, 1582, 1586, 1921, 1997
NlaIV	GGNNCC	5 cut(s)	324, 1035, 1099, 1425, 1666
NmeAIII	GCCGAG	1 cut(s)	93
NmuCI	GTSAC	5 cut(s)	412, 895, 1159, 1180, 2121
NsiI	ATGCAT	3 cut(s)	1051, 1269, 1584
NspI	RCATGY	1 cut(s)	1327
NspV	TTCGAA	1 cut(s)	873
PaeR7I	CTCGAG	1 cut(s)	461
PaqCI	CACCTGC	1 cut(s)	1310
PceI	AGGCCT	1 cut(s)	2216
PcsI	WCGNNNNNNNCGW	1 cut(s)	1245
PdmI	GAANNNNTTC	3 cut(s)	757, 879, 1979
PfeI	GAWTC	3 cut(s)	1136, 1145, 1495
PflMI	CCANNNNNTGG	1 cut(s)	1912
PinAI	ACCGGT	1 cut(s)	302
PkrI	GCNGC	2 cut(s)	1076, 1354
PleI	GAGTC	3 cut(s)	222, 1684, 1874
PpsI	GAGTC	3 cut(s)	222, 1684, 1874
PpuMI	RGGWCCY	1 cut(s)	365
PshBI	ATTAAT	2 cut(s)	1380, 1409
Psp124BI	GAGCTC	3 cut(s)	531, 1290, 1729
Psp5II	RGGWCCY	1 cut(s)	365
Psp6I	CCWGG	1 cut(s)	1874
PspEI	GGTNACC	1 cut(s)	2121
PspFI	CCCAGC	1 cut(s)	817
PspGI	CCWGG	1 cut(s)	1874
PspN4I	GGNNCC	5 cut(s)	324, 1035, 1099, 1425, 1666
PspPI	GGNCC	7 cut(s)	322, 365, 493, 791, 1665, 1773, 2042
PspPPI	RGGWCCY	1 cut(s)	365
PsrI	GAACNNNNNNTAC	4 cut(s)	189, 221, 943, 975
PstI	CTGCAG	2 cut(s)	1079, 1333
PstNI	CAGNNNCTG	1 cut(s)	368
PvuII	CAGCTG	1 cut(s)	821
RsaI	GTAC	5 cut(s)	307, 606, 1112, 1392, 1716
RsaNI	GTAC	5 cut(s)	306, 605, 1111, 1391, 1715
RseI	CAYNNNNRTG	5 cut(s)	248, 986, 1124, 1998, 2225
SacI	GAGCTC	3 cut(s)	531, 1290, 1729
SatI	GCNGC	2 cut(s)	1075, 1353
Sau3AI	GATC	9 cut(s)	294, 560, 836, 930, 943, 1222, 1335, 1507, 1753
Sau96I	GGNCC	7 cut(s)	322, 365, 493, 791, 1665, 1773, 2042
SchI	GAGTC	3 cut(s)	222, 1685, 1874
ScrFI	CCNGG	5 cut(s)	144, 145, 689, 1123, 1876
SduI	GDGCHC	3 cut(s)	531, 1290, 1729
SfaNI	GCATC	7 cut(s)	1036, 1314, 1339, 1951, 2201, 2220, 2243
SfcI	CTRYAG	4 cut(s)	180, 1075, 1329, 1815
Sfr274I	CTCGAG	1 cut(s)	461
SfuI	TTCGAA	1 cut(s)	873
SinI	GGWCC	5 cut(s)	322, 365, 1665, 1773, 2042
SlaI	CTCGAG	1 cut(s)	461
SmaI	CCCGGG	1 cut(s)	145
SmiMI	CAYNNNNRTG	5 cut(s)	248, 986, 1124, 1998, 2225
SmlI	CTYRAG	2 cut(s)	461, 532
SmoI	CTYRAG	2 cut(s)	461, 532
Sse9I	AATT	9 cut(s)	54, 198, 481, 564, 854, 1293, 1406, 1540, 2156
SseBI	AGGCCT	1 cut(s)	2216
SsiI	CCGC	3 cut(s)	360, 644, 2045
SspI	AATATT	2 cut(s)	651, 999
SspMI	CTAG	1 cut(s)	1170
SstI	GAGCTC	3 cut(s)	531, 1290, 1729
StuI	AGGCCT	1 cut(s)	2216
StyD4I	CCNGG	5 cut(s)	142, 143, 687, 1121, 1874
StyI	CCWWGG	4 cut(s)	121, 1008, 1093, 1917
TaaI	ACNGT	5 cut(s)	188, 409, 1462, 1900, 2134
TaiI	ACGT	3 cut(s)	289, 320, 633
TaqII	GACCGA	1 cut(s)	1761
TasI	AATT	9 cut(s)	54, 198, 481, 564, 854, 1293, 1406, 1540, 2156
TatI	WGTACW	3 cut(s)	604, 1390, 1714
TfiI	GAWTC	3 cut(s)	1136, 1145, 1495
TscAI	CASTG	3 cut(s)	340, 1338, 1717
TseFI	GTSAC	5 cut(s)	412, 895, 1159, 1180, 2121
TseI	GCWGC	2 cut(s)	1074, 1352
Tsp45I	GTSAC	5 cut(s)	412, 895, 1159, 1180, 2121
TspDTI	ATGAA	8 cut(s)	19, 59, 558, 597, 773, 1599, 1631, 1941
TspGWI	ACGGA	3 cut(s)	164, 1008, 1726
TspMI	CCCGGG	1 cut(s)	143
TspRI	CASTG	3 cut(s)	340, 1338, 1717
Van91I	CCANNNNNTGG	1 cut(s)	1912
Vha464I	CTTAAG	1 cut(s)	532
VpaK11BI	GGWCC	5 cut(s)	322, 365, 1665, 1773, 2042
VspI	ATTAAT	2 cut(s)	1380, 1409
XagI	CCTNNNNNAGG	1 cut(s)	461
XapI	RAATTY	3 cut(s)	854, 1293, 2156
XceI	RCATGY	1 cut(s)	1327
XcmI	CCANNNNNNNNNTGG	2 cut(s)	666, 733
XhoI	CTCGAG	1 cut(s)	461
XmaI	CCCGGG	1 cut(s)	143
XmiI	GTMKAC	2 cut(s)	106, 183
XmnI	GAANNNNTTC	3 cut(s)	757, 879, 1979
XspI	CTAG	1 cut(s)	1170
Zsp2I	ATGCAT	3 cut(s)	1051, 1269, 1584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.