Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Forward (+)

36025998 .. 36029689

Gene Structure 3692 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1267300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGTCATCGCCTCTACTTCTTATTTTTGTAGTGACTGCCTTCTGGATCTTTAGTGTGGTGACACCGTATCAAACAGGAATGCAGAAGAGGATGACCGATCCGGTGACCATATGCACGGTGTTTCCGCCTCGCGTTAATTCGCTTGGCTTGTGGAACAAATCGGATGCTTGGCCGGTGCATGTTAAAGACCCGCTTGGGATTGTGCATTTCACCTTTCGACTGCAGAACTACACCCTCCCAATTCTGGAATTTGAAATTACTCTCATCTATGTTCTAACCCAACTCACCCATTTGTTTCTCAAGTTATTTGGCCTCCCGGTGTTTGCCGCACAACTTCTGGCTGGATTACTCCTTGGTGATTTAGGACTTGACAGAGTGGTCCGACATTCAAGTCGTGGCTTCAGTTTGAATCCCATATTTCCTTTTCCTAATCAAGAAGTACTCAACATAATATCACTGTCTGGCTACACACTCTTCACCTTCTTGTTTGGAGTAAAAATGGACGTCGGATTAGTATATAAAACCGGAAGAAAGGCCATCTACACCGGTGTTTTAACCTTGATAGTGCCTCTAATTGCCGCCACTGCAACCCTTCTCATCCTCACCTTCCAGTGGTCCTTACAAGCCGAGGAAATTGTTCAGCTTCTTTTCATAACATCAATGCACTCTCTGTCTCCGTCCCCCGTCATCCATTGCCTCCTCACCGAGCTCAGAATCTTAAACTCTGAGCTCGGCCGGCTCGCATTATCATCGGCACTTGTCAGTGACCTCTCCAGCATTTCACTCACCGGTGTTTCAACCATAATGACAGTCATGCAGCAAGATCCGGATAAGCCCCTTATCGACTTGACGATGATGATAGTCTTCACCTTGTTTGTTTACTACGTTTGTCGTCCAGTTATGTTGTGGATGGTGAGAAAGACACCGGAGGGCAAATCCGTTAAGGACTCGTACATATCAATAATCCTGCTGGCTGTTTTTGGATCTGGATTGATTTCCCATGAAGCTCGTCTGTCCATTGTAGTGGGCCCTTTAATCTTGGGATTGGCTGTGCCAGATGGGCCCCCACTGGGAGCTACACTGGTCAGAAAGCTTGACTTTATGGTCAATAGGGTTTTTATGCCGGTTTTTGTGACGACGTCCATGATGAGGGTGGTTGTACATCAGGAGGTGCCTGATGGTGACCAAACGGCAGTTCGTGCCACAGCAATCATCACTCTTGTGATCTTCTTGAGCAAATTTGTGGCCTCTTTGGTGACTCCTTTGTGTTGCAATATGCCTCTCAAGCATGCTGTGGCACTAGCACTCGTAATGACCAGCAAAGGCGTTGTCGAGATAGCCGCATACACCATTGCTAGAGACACTTGGGTTATTGTTCATGGGGTCTTTAATTACATGATGTTCACCGTCGTATTTAGTGCAATTGTCGTGCCACCTCTTGTGAAGCTCTTGTACGATCCTAAGATAAAATACGCCGGCTACCAACAAAGAAACCTTATGAATGACAAACCCAATTCCGACCTACGAATCCTTGCGTGCATCAACAGAGCCGATAACACCCCCGCCATCATCAATCTTCTCGACGCCGCGTGCCCTACTCGAGAAAGCTCCATCACCCTCTACGTCCTTCACCTCATCGAGCTAGTTGCCCGAAGCACGCCCATCTTCTTCGCCCACGATATACACAAAAGGGACCTCTCCAACTACTCGTACTCCGACAATGTCATCCGCTCGTTTACTCAGTTTATGAGAGACAACGATGGAGCTGTCTCCGTTAATGTGTTTACAGTGGTCTCTCCTCCCCAATACATGTATGACGACATATGCACCCTCGCGCTGGACAAGGACACGTCGCTCATAATCCTTCCGTTTCATCGGAAGTGGTCGACCACTGACGGGTCTATTGAGTCGGAAAACCAAAACGTAAGGGATCTCAATCGTAGGGTACTTAAAATCGCGCCATGTTCGGTTGCCATTCTTGTGAACCGTGGTCACATAAGATGCACCAACTCCTCAGCTGAAGGGTATAGAGTTGCGGTGGTTTTTCTCGGAGGGAAGGACGATAGAGAGGCGCTTACTCTCGCGAAACGGATGGTGAAGGACGCGTGCATCAGCCTGACGGTGATTCATCTGGTGGACGCATCAGACCACGAGGAGAGTCACAACAAATGGGAGTGGGTGCAGGACAATGAGATGTTGAAGGAAGTAAAGCAGAAGAGTATAGGGTATGTGAGTTACGTGGAAGAGAATGTACAGGATGGGACTCAAACGACAAGGAAAATACGGTCGTTGATGGACGACGATGAATACGACCTGCTCATCGTCGGGAGAAGATATAAGGTGAAAAGCCCTCAGACGTTGGGTCTTGAGGACTGGAGTGAGTTCCCGGAGCTTGGGGTTATCGGCGACATGCTTTCCTCAGCGGACTTTCGCTGCAAATCTAGTGTGTTGGTGGTTCAGCAACAGCAACAACGTAGTAATGCCACTATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

831

Amino Acids

92.81

Weight (kDa)

7.26

Isoelectric Point (pI)

40.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	91 - 481	2.3e-28	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	538 - 671	2.5e-13	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	677 - 822	1.4e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	377, 390, 1103
AatII	GACGTC	2 cut(s)	507, 1142
Acc36I	ACCTGC	1 cut(s)	2322
AccB1I	GGYRCC	1 cut(s)	1171
AccBSI	CCGCTC	1 cut(s)	1731
AccI	GTMKAC	1 cut(s)	1886
AccII	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
AccIII	TCCGGA	1 cut(s)	826
AclWI	GGATC	6 cut(s)	53, 92, 818, 991, 1451, 1938
AcoI	YGGCCR	2 cut(s)	170, 733
AcsI	RAATTY	2 cut(s)	248, 1238
AcuI	CTGAAG	2 cut(s)	385, 2040
AcyI	GRCGYC	3 cut(s)	504, 1139, 1584
AfaI	GTAC	7 cut(s)	441, 953, 1161, 1454, 1712, 1947, 2253
AfiI	CCNNNNNNNGG	7 cut(s)	244, 612, 1070, 1149, 1837, 1896, 2393
AflIII	ACRYGT	3 cut(s)	1809, 1848, 2103
AgeI	ACCGGT	2 cut(s)	545, 788
AgsI	TTSAA	5 cut(s)	254, 390, 409, 798, 2200
AhdI	GACNNNNNGTC	1 cut(s)	2361
AjiI	CACGTC	1 cut(s)	1851
AjuI	GAANNNNNNNTTGG	2 cut(s)	1179, 1211
AleI	CACNNNNGTG	1 cut(s)	1220
Alw21I	GWGCWC	2 cut(s)	711, 732
Alw26I	GTCTC	5 cut(s)	678, 1353, 1746, 1774, 1798
AlwI	GGATC	6 cut(s)	53, 92, 818, 991, 1451, 1938
Ama87I	CYCGRG	1 cut(s)	1599
Aor13HI	TCCGGA	1 cut(s)	826
AoxI	GGCC	7 cut(s)	170, 310, 534, 733, 1027, 1061, 1245
ApaI	GGGCCC	2 cut(s)	1031, 1065
ApeKI	GCWGC	2 cut(s)	817, 2433
ApoI	RAATTY	2 cut(s)	248, 1238
ArsI	GACNNNNNNTTYG	2 cut(s)	1314, 1346
AsiGI	ACCGGT	2 cut(s)	545, 788
Asp700I	GAANNNNTTC	1 cut(s)	636
AspLEI	GCGC	3 cut(s)	1837, 1960, 2074
AspS9I	GGNCC	7 cut(s)	379, 615, 1027, 1028, 1061, 1062, 1693
AsuC2I	CCSGG	2 cut(s)	317, 2387
AvaI	CYCGRG	1 cut(s)	1599
AvaII	GGWCC	3 cut(s)	379, 615, 1693
BaeGI	GKGCMC	3 cut(s)	1031, 1065, 1595
BanI	GGYRCC	1 cut(s)	1171
BanII	GRGCYC	4 cut(s)	711, 732, 1031, 1065
BarI	GAAGNNNNNNTAC	4 cut(s)	1436, 1468, 2235, 2267
BauI	CACGAG	1 cut(s)	2150
BbsI	GAAGAC	1 cut(s)	856
Bbv12I	GWGCWC	2 cut(s)	711, 732
BbvCI	CCTCAGC	2 cut(s)	2014, 2419
BbvI	GCAGC	2 cut(s)	829, 2420
BceAI	ACGGC	1 cut(s)	1206
BcgI	CGANNNNNNTGC	4 cut(s)	732, 766, 1628, 1662
BcnI	CCSGG	2 cut(s)	317, 2387
BcoDI	GTCTC	5 cut(s)	678, 1353, 1746, 1774, 1798
BfaI	CTAG	4 cut(s)	1301, 1356, 1643, 2442
BfmI	CTRYAG	1 cut(s)	221
BfoI	RGCGCY	1 cut(s)	2075
BfuAI	ACCTGC	1 cut(s)	2322
BglI	GCCNNNNNGGC	1 cut(s)	1060
BisI	GCNGC	6 cut(s)	327, 579, 818, 1341, 1587, 2434
BlsI	GCNGC	6 cut(s)	328, 580, 819, 1342, 1588, 2435
BmcAI	AGTACT	1 cut(s)	441
Bme1390I	CCNGG	2 cut(s)	317, 2387
Bme18I	GGWCC	3 cut(s)	379, 615, 1693
BmeRI	GACNNNNNGTC	1 cut(s)	2361
BmeT110I	CYCGRG	1 cut(s)	1599
BmgBI	CACGTC	1 cut(s)	1851
BmgT120I	GGNCC	7 cut(s)	379, 615, 1027, 1028, 1061, 1062, 1693
BmiI	GGNNCC	5 cut(s)	1029, 1063, 1064, 1173, 1694
BmrFI	CCNGG	2 cut(s)	317, 2387
BmrI	ACTGGG	1 cut(s)	1079
BmsI	GCATC	5 cut(s)	154, 1548, 1991, 2118, 2150
BmuI	ACTGGG	1 cut(s)	1079
BpiI	GAAGAC	1 cut(s)	856
BpmI	CTGGAG	2 cut(s)	757, 2395
Bpu10I	CCTNAGC	2 cut(s)	2014, 2419
BpuEI	CTTGAG	4 cut(s)	284, 1252, 1268, 2387
BpuMI	CCSGG	2 cut(s)	317, 2387
BsaAI	YACGTR	1 cut(s)	2239
BsaBI	GATNNNNATC	1 cut(s)	1935
BsaHI	GRCGYC	3 cut(s)	504, 1139, 1584
BsaI	GGTCTC	1 cut(s)	1798
BsaJI	CCNNGG	3 cut(s)	352, 627, 1987
BsaWI	WCCGGW	6 cut(s)	100, 524, 545, 788, 826, 925
BsaXI	ACNNNNNCTCC	2 cut(s)	1697, 1727
Bsc4I	CCNNNNNNNGG	7 cut(s)	244, 612, 1070, 1149, 1837, 1896, 2393
Bse118I	RCCGGY	6 cut(s)	172, 545, 735, 788, 1123, 1475
Bse1I	ACTGG	5 cut(s)	610, 896, 1074, 1086, 2378
Bse3DI	GCAATG	2 cut(s)	691, 1350
Bse8I	GATNNNNATC	1 cut(s)	1935
BseAI	TCCGGA	1 cut(s)	826
BseDI	CCNNGG	3 cut(s)	352, 627, 1987
BseGI	GGATG	8 cut(s)	96, 169, 597, 687, 915, 1725, 2097, 2263
BseJI	GATNNNNATC	1 cut(s)	1935
BseLI	CCNNNNNNNGG	7 cut(s)	244, 612, 1070, 1149, 1837, 1896, 2393
BseMI	GCAATG	2 cut(s)	691, 1350
BseMII	CTCAG	6 cut(s)	717, 724, 1754, 2028, 2366, 2433
BseNI	ACTGG	5 cut(s)	610, 896, 1074, 1086, 2378
BseRI	GAGGAG	4 cut(s)	689, 1788, 2002, 2168
BseSI	GKGCMC	3 cut(s)	1031, 1065, 1595
BseX3I	CGGCCG	1 cut(s)	733
BseXI	GCAGC	2 cut(s)	829, 2420
BsgI	GTGCAG	1 cut(s)	2201
Bsh1236I	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
Bsh1285I	CGRYCG	2 cut(s)	736, 2288
BshFI	GGCC	7 cut(s)	172, 312, 536, 735, 1029, 1063, 1247
BshNI	GGYRCC	1 cut(s)	1171
BshTI	ACCGGT	2 cut(s)	545, 788
BsiEI	CGRYCG	2 cut(s)	736, 2288
BsiHKAI	GWGCWC	2 cut(s)	711, 732
BsiHKCI	CYCGRG	1 cut(s)	1599
BslFI	GGGAC	3 cut(s)	664, 1706, 2275
BslI	CCNNNNNNNGG	7 cut(s)	244, 612, 1070, 1149, 1837, 1896, 2393
BsmAI	GTCTC	5 cut(s)	678, 1353, 1746, 1774, 1798
BsmFI	GGGAC	3 cut(s)	664, 1706, 2275
BsmI	GAATGC	1 cut(s)	84
BsnI	GGCC	7 cut(s)	172, 312, 536, 735, 1029, 1063, 1247
Bso31I	GGTCTC	1 cut(s)	1798
BsoBI	CYCGRG	1 cut(s)	1599
Bsp120I	GGGCCC	2 cut(s)	1027, 1061
Bsp1286I	GDGCHC	5 cut(s)	711, 732, 1031, 1065, 1595
Bsp13I	TCCGGA	1 cut(s)	826
Bsp1407I	TGTACA	2 cut(s)	1159, 2251
Bsp143I	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
Bsp68I	TCGCGA	1 cut(s)	2084
BspANI	GGCC	7 cut(s)	172, 312, 536, 735, 1029, 1063, 1247
BspCNI	CTCAG	6 cut(s)	718, 723, 1753, 2027, 2365, 2432
BspEI	TCCGGA	1 cut(s)	826
BspFNI	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
BspLI	GGNNCC	5 cut(s)	1029, 1063, 1064, 1173, 1694
BspMAI	CTGCAG	1 cut(s)	225
BspMI	ACCTGC	1 cut(s)	2322
BspPI	GGATC	6 cut(s)	53, 92, 818, 991, 1451, 1938
BspT107I	GGYRCC	1 cut(s)	1171
BspTNI	GGTCTC	1 cut(s)	1798
BsrBI	CCGCTC	1 cut(s)	1731
BsrDI	GCAATG	2 cut(s)	691, 1350
BsrFI	RCCGGY	6 cut(s)	172, 545, 735, 788, 1123, 1475
BsrGI	TGTACA	2 cut(s)	1159, 2251
BsrI	ACTGG	5 cut(s)	610, 896, 1074, 1086, 2378
BssAI	RCCGGY	6 cut(s)	172, 545, 735, 788, 1123, 1475
BssECI	CCNNGG	3 cut(s)	352, 627, 1987
BssMI	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
BssNI	GRCGYC	3 cut(s)	504, 1139, 1584
BssSI	CACGAG	1 cut(s)	2150
BssT1I	CCWWGG	1 cut(s)	352
Bst2BI	CACGAG	1 cut(s)	2150
Bst4CI	ACNGT	9 cut(s)	66, 118, 459, 811, 1408, 1789, 1988, 2122, 2286
Bst6I	CTCTTC	4 cut(s)	80, 479, 2210, 2238
BstACI	GRCGYC	3 cut(s)	504, 1139, 1584
BstAPI	GCANNNNNTGC	1 cut(s)	1199
BstAUI	TGTACA	2 cut(s)	1159, 2251
BstBAI	YACGTR	1 cut(s)	2239
BstC8I	GCNNGC	9 cut(s)	737, 741, 972, 1290, 1477, 1537, 1591, 1658, 2107
BstDEI	CTNAG	7 cut(s)	710, 726, 1461, 1740, 2014, 2352, 2419
BstDSI	CCRYGG	1 cut(s)	1987
BstEII	GGTNACC	2 cut(s)	103, 1181
BstF5I	GGATG	8 cut(s)	96, 169, 597, 687, 915, 1725, 2097, 2263
BstFNI	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
BstH2I	RGCGCY	1 cut(s)	2075
BstHHI	GCGC	3 cut(s)	1837, 1960, 2074
BstKTI	GATC	7 cut(s)	48, 100, 826, 986, 1227, 1459, 1933
BstMAI	GTCTC	5 cut(s)	678, 1353, 1746, 1774, 1798
BstMBI	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
BstMCI	CGRYCG	2 cut(s)	736, 2288
BstMWI	GCNNNNNNNGC	5 cut(s)	584, 736, 1060, 1199, 1285
BstNSI	RCATGY	4 cut(s)	182, 1292, 1813, 2413
BstPI	GGTNACC	2 cut(s)	103, 1181
BstSCI	CCNGG	2 cut(s)	315, 2385
BstSFI	CTRYAG	1 cut(s)	221
BstSLI	GKGCMC	3 cut(s)	1031, 1065, 1595
BstUI	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
BstV1I	GCAGC	2 cut(s)	829, 2420
BstV2I	GAAGAC	1 cut(s)	856
BstX2I	RGATCY	4 cut(s)	45, 823, 983, 1930
BstXI	CCANNNNNNTGG	1 cut(s)	1024
BstYI	RGATCY	4 cut(s)	45, 823, 983, 1930
BstZI	CGGCCG	1 cut(s)	733
BsuRI	GGCC	7 cut(s)	172, 312, 536, 735, 1029, 1063, 1247
BtgI	CCRYGG	1 cut(s)	1987
BtrI	CACGTC	1 cut(s)	1851
BtsCI	GGATG	8 cut(s)	96, 169, 597, 687, 915, 1725, 2097, 2263
BtsI	GCAGTG	1 cut(s)	582
BtsIMutI	CAGTG	8 cut(s)	455, 582, 617, 769, 1067, 1079, 1794, 1890
BtuMI	TCGCGA	1 cut(s)	2084
BveI	ACCTGC	1 cut(s)	2322
Cac8I	GCNNGC	9 cut(s)	737, 741, 972, 1290, 1477, 1537, 1591, 1658, 2107
CfoI	GCGC	3 cut(s)	1837, 1960, 2074
Cfr10I	RCCGGY	6 cut(s)	172, 545, 735, 788, 1123, 1475
Cfr13I	GGNCC	7 cut(s)	379, 615, 1027, 1028, 1061, 1062, 1693
CseI	GACGC	3 cut(s)	1592, 2111, 2147
Csp6I	GTAC	7 cut(s)	440, 952, 1160, 1453, 1711, 1946, 2252
CspAI	ACCGGT	2 cut(s)	545, 788
CviQI	GTAC	7 cut(s)	440, 952, 1160, 1453, 1711, 1946, 2252
DdeI	CTNAG	7 cut(s)	710, 726, 1461, 1740, 2014, 2352, 2419
DpnI	GATC	7 cut(s)	47, 99, 825, 985, 1226, 1458, 1932
DpnII	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
DrdI	GACNNNNNNGTC	3 cut(s)	377, 390, 1103
DriI	GACNNNNNGTC	1 cut(s)	2361
DseDI	GACNNNNNNGTC	3 cut(s)	377, 390, 1103
EaeI	YGGCCR	2 cut(s)	170, 733
EagI	CGGCCG	1 cut(s)	733
Eam1104I	CTCTTC	4 cut(s)	80, 479, 2210, 2238
Eam1105I	GACNNNNNGTC	1 cut(s)	2361
EarI	CTCTTC	4 cut(s)	80, 479, 2210, 2238
EciI	GGCGGA	1 cut(s)	114
Ecl136II	GAGCTC	2 cut(s)	709, 730
EclXI	CGGCCG	1 cut(s)	733
Eco130I	CCWWGG	1 cut(s)	352
Eco24I	GRGCYC	4 cut(s)	711, 732, 1031, 1065
Eco31I	GGTCTC	1 cut(s)	1798
Eco47I	GGWCC	3 cut(s)	379, 615, 1693
Eco52I	CGGCCG	1 cut(s)	733
Eco53kI	GAGCTC	2 cut(s)	709, 730
Eco57I	CTGAAG	2 cut(s)	385, 2040
Eco88I	CYCGRG	1 cut(s)	1599
Eco91I	GGTNACC	2 cut(s)	103, 1181
EcoICRI	GAGCTC	2 cut(s)	709, 730
EcoO109I	RGGNCCY	3 cut(s)	1028, 1062, 1693
EcoO65I	GGTNACC	2 cut(s)	103, 1181
EcoT14I	CCWWGG	1 cut(s)	352
EcoT38I	GRGCYC	4 cut(s)	711, 732, 1031, 1065
ErhI	CCWWGG	1 cut(s)	352
FalI	AAGNNNNNCTT	4 cut(s)	177, 209, 1082, 1114
FaqI	GGGAC	3 cut(s)	664, 1706, 2275
FauI	CCCGC	2 cut(s)	198, 1570
FauNDI	CATATG	2 cut(s)	110, 1823
FblI	GTMKAC	1 cut(s)	1886
Fnu4HI	GCNGC	6 cut(s)	327, 579, 818, 1341, 1587, 2434
FokI	GGATG	8 cut(s)	103, 176, 584, 674, 922, 1712, 2104, 2270
FriOI	GRGCYC	4 cut(s)	711, 732, 1031, 1065
Fsp4HI	GCNGC	6 cut(s)	327, 579, 818, 1341, 1587, 2434
FspBI	CTAG	4 cut(s)	1301, 1356, 1643, 2442
GlaI	GCGC	3 cut(s)	1836, 1959, 2073
GluI	GCNGC	6 cut(s)	327, 579, 818, 1341, 1587, 2434
GsuI	CTGGAG	2 cut(s)	757, 2395
HaeII	RGCGCY	1 cut(s)	2075
HaeIII	GGCC	7 cut(s)	172, 312, 536, 735, 1029, 1063, 1247
HgaI	GACGC	3 cut(s)	1592, 2111, 2147
HhaI	GCGC	3 cut(s)	1837, 1960, 2074
Hin1I	GRCGYC	3 cut(s)	504, 1139, 1584
Hin6I	GCGC	3 cut(s)	1835, 1958, 2072
HinP1I	GCGC	3 cut(s)	1835, 1958, 2072
HincII	GTYRAC	1 cut(s)	1887
HindII	GTYRAC	1 cut(s)	1887
HindIII	AAGCTT	1 cut(s)	1091
HinfI	GANTC	9 cut(s)	409, 714, 947, 1258, 1527, 1907, 2125, 2158, 2263
Hpy166II	GTNNAC	7 cut(s)	880, 1404, 1737, 1785, 1887, 1984, 2137
Hpy8I	GTNNAC	7 cut(s)	880, 1404, 1737, 1785, 1887, 1984, 2137
Hpy99I	CGWCG	7 cut(s)	509, 1141, 1412, 1586, 1855, 2303, 2327
HpyCH4III	ACNGT	9 cut(s)	66, 118, 459, 811, 1408, 1789, 1988, 2122, 2286
HpyCH4IV	ACGT	9 cut(s)	504, 885, 1139, 1623, 1850, 1923, 2238, 2357, 2473
HpyF10VI	GCNNNNNNNGC	5 cut(s)	584, 736, 1060, 1199, 1285
HpyF3I	CTNAG	7 cut(s)	710, 726, 1461, 1740, 2014, 2352, 2419
HpySE526I	ACGT	9 cut(s)	504, 885, 1139, 1623, 1850, 1923, 2238, 2357, 2473
Hsp92I	GRCGYC	3 cut(s)	504, 1139, 1584
HspAI	GCGC	3 cut(s)	1835, 1958, 2072
Kpn2I	TCCGGA	1 cut(s)	826
KroI	GCCGGC	2 cut(s)	735, 1475
KroNI	GCCGGC	2 cut(s)	737, 1477
Kzo9I	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
LmnI	GCTCC	4 cut(s)	1073, 1613, 1763, 2389
Lsp1109I	GCAGC	2 cut(s)	829, 2420
LweI	GCATC	5 cut(s)	154, 1548, 1991, 2118, 2150
MaeI	CTAG	4 cut(s)	1301, 1356, 1643, 2442
MaeII	ACGT	9 cut(s)	504, 885, 1139, 1623, 1850, 1923, 2238, 2357, 2473
MalI	GATC	7 cut(s)	47, 99, 825, 985, 1226, 1458, 1932
MbiI	CCGCTC	1 cut(s)	1731
MboI	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
MfeI	CAATTG	1 cut(s)	1422
MflI	RGATCY	4 cut(s)	45, 823, 983, 1930
MhlI	GDGCHC	5 cut(s)	711, 732, 1031, 1065, 1595
MluI	ACGCGT	1 cut(s)	2103
MlyI	GAGTC	5 cut(s)	941, 1252, 1916, 2167, 2257
MmeI	TCCRAC	6 cut(s)	406, 487, 1542, 1725, 1740, 1890
MroI	TCCGGA	1 cut(s)	826
MroNI	GCCGGC	2 cut(s)	735, 1475
MroXI	GAANNNNTTC	1 cut(s)	636
MseI	TTAA	9 cut(s)	135, 183, 554, 719, 942, 1034, 1389, 1776, 1950
MslI	CAYNNNNRTG	3 cut(s)	1022, 1220, 1979
MspA1I	CMGCKG	2 cut(s)	2018, 2423
MspR9I	CCNGG	2 cut(s)	317, 2387
MunI	CAATTG	1 cut(s)	1422
Mva1269I	GAATGC	1 cut(s)	84
MvnI	CGCG	6 cut(s)	132, 1589, 1835, 1958, 2084, 2105
MwoI	GCNNNNNNNGC	5 cut(s)	584, 736, 1060, 1199, 1285
NaeI	GCCGGC	2 cut(s)	737, 1477
NciI	CCSGG	2 cut(s)	317, 2387
NdeI	CATATG	2 cut(s)	110, 1823
NdeII	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
NgoMIV	GCCGGC	2 cut(s)	735, 1475
NlaIV	GGNNCC	5 cut(s)	1029, 1063, 1064, 1173, 1694
NmeAIII	GCCGAG	2 cut(s)	652, 711
NmuCI	GTSAC	9 cut(s)	31, 58, 103, 764, 1132, 1181, 1255, 1991, 2159
NruI	TCGCGA	1 cut(s)	2084
NspI	RCATGY	4 cut(s)	182, 1292, 1813, 2413
OliI	CACNNNNGTG	1 cut(s)	1220
PaeI	GCATGC	1 cut(s)	1292
PaeR7I	CTCGAG	1 cut(s)	1599
PciI	ACATGT	1 cut(s)	1809
PcsI	WCGNNNNNNNCGW	1 cut(s)	2307
PctI	GAATGC	1 cut(s)	84
PdiI	GCCGGC	2 cut(s)	737, 1477
PdmI	GAANNNNTTC	1 cut(s)	636
PfeI	GAWTC	4 cut(s)	409, 714, 1527, 2125
PflFI	GACNNNGTC	2 cut(s)	1138, 1721
PfoI	TCCNGGA	1 cut(s)	2385
PinAI	ACCGGT	2 cut(s)	545, 788
PkrI	GCNGC	6 cut(s)	328, 580, 819, 1342, 1588, 2435
PleI	GAGTC	5 cut(s)	941, 1252, 1915, 2166, 2257
PpsI	GAGTC	5 cut(s)	941, 1252, 1915, 2166, 2257
Ppu21I	YACGTR	1 cut(s)	2239
PpuMI	RGGWCCY	1 cut(s)	1693
PscI	ACATGT	1 cut(s)	1809
Psp124BI	GAGCTC	2 cut(s)	711, 732
Psp5II	RGGWCCY	1 cut(s)	1693
PspEI	GGTNACC	2 cut(s)	103, 1181
PspN4I	GGNNCC	5 cut(s)	1029, 1063, 1064, 1173, 1694
PspOMI	GGGCCC	2 cut(s)	1027, 1061
PspPI	GGNCC	7 cut(s)	379, 615, 1027, 1028, 1061, 1062, 1693
PspPPI	RGGWCCY	1 cut(s)	1693
PstI	CTGCAG	1 cut(s)	225
PsuI	RGATCY	4 cut(s)	45, 823, 983, 1930
PsyI	GACNNNGTC	2 cut(s)	1138, 1721
PvuII	CAGCTG	1 cut(s)	2018
RruI	TCGCGA	1 cut(s)	2084
RsaI	GTAC	7 cut(s)	441, 953, 1161, 1454, 1712, 1947, 2253
RsaNI	GTAC	7 cut(s)	440, 952, 1160, 1453, 1711, 1946, 2252
RseI	CAYNNNNRTG	3 cut(s)	1022, 1220, 1979
SacI	GAGCTC	2 cut(s)	711, 732
SalI	GTCGAC	1 cut(s)	1885
SaqAI	TTAA	9 cut(s)	135, 183, 554, 719, 942, 1034, 1389, 1776, 1950
SatI	GCNGC	6 cut(s)	327, 579, 818, 1341, 1587, 2434
Sau3AI	GATC	7 cut(s)	45, 97, 823, 983, 1224, 1456, 1930
Sau96I	GGNCC	7 cut(s)	379, 615, 1027, 1028, 1061, 1062, 1693
ScaI	AGTACT	1 cut(s)	441
SchI	GAGTC	5 cut(s)	941, 1252, 1916, 2167, 2257
ScrFI	CCNGG	2 cut(s)	317, 2387
SduI	GDGCHC	5 cut(s)	711, 732, 1031, 1065, 1595
SfaNI	GCATC	5 cut(s)	154, 1548, 1991, 2118, 2150
SfcI	CTRYAG	1 cut(s)	221
Sfr274I	CTCGAG	1 cut(s)	1599
SgrAI	CRCCGGYG	2 cut(s)	545, 788
SinI	GGWCC	3 cut(s)	379, 615, 1693
SlaI	CTCGAG	1 cut(s)	1599
SmiMI	CAYNNNNRTG	3 cut(s)	1022, 1220, 1979
SmlI	CTYRAG	5 cut(s)	299, 1231, 1283, 1599, 2366
SmoI	CTYRAG	5 cut(s)	299, 1231, 1283, 1599, 2366
SphI	GCATGC	1 cut(s)	1292
SspMI	CTAG	4 cut(s)	1301, 1356, 1643, 2442
SstI	GAGCTC	2 cut(s)	711, 732
StyD4I	CCNGG	2 cut(s)	315, 2385
StyI	CCWWGG	1 cut(s)	352
TaaI	ACNGT	9 cut(s)	66, 118, 459, 811, 1408, 1789, 1988, 2122, 2286
TaiI	ACGT	9 cut(s)	507, 888, 1142, 1626, 1853, 1926, 2241, 2360, 2476
TaqI	TCGA	7 cut(s)	217, 843, 1332, 1581, 1600, 1638, 1886
TaqII	GACCGA	1 cut(s)	110
TatI	WGTACW	3 cut(s)	439, 1159, 2251
TauI	GCSGC	4 cut(s)	329, 581, 1343, 1589
TfiI	GAWTC	4 cut(s)	409, 714, 1527, 2125
Tru1I	TTAA	9 cut(s)	135, 183, 554, 719, 942, 1034, 1389, 1776, 1950
Tru9I	TTAA	9 cut(s)	135, 183, 554, 719, 942, 1034, 1389, 1776, 1950
TscAI	CASTG	8 cut(s)	462, 589, 617, 769, 1074, 1086, 1794, 1897
TseFI	GTSAC	9 cut(s)	31, 58, 103, 764, 1132, 1181, 1255, 1991, 2159
TseI	GCWGC	2 cut(s)	817, 2433
Tsp45I	GTSAC	9 cut(s)	31, 58, 103, 764, 1132, 1181, 1255, 1991, 2159
TspDTI	ATGAA	7 cut(s)	640, 1017, 1367, 1514, 1862, 2117, 2319
TspGWI	ACGGA	5 cut(s)	666, 928, 1762, 1857, 2104
TspRI	CASTG	8 cut(s)	462, 589, 617, 769, 1074, 1086, 1794, 1897
Tth111I	GACNNNGTC	2 cut(s)	1138, 1721
VpaK11BI	GGWCC	3 cut(s)	379, 615, 1693
XapI	RAATTY	2 cut(s)	248, 1238
XceI	RCATGY	4 cut(s)	182, 1292, 1813, 2413
XcmI	CCANNNNNNNNNTGG	2 cut(s)	903, 1150
XhoI	CTCGAG	1 cut(s)	1599
XmiI	GTMKAC	1 cut(s)	1886
XmnI	GAANNNNTTC	1 cut(s)	636
XspI	CTAG	4 cut(s)	1301, 1356, 1643, 2442
ZraI	GACGTC	2 cut(s)	505, 1140
ZrmI	AGTACT	1 cut(s)	441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.