Rh5DG501700

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001192
Physical Location & Seq
Reverse (-)
78609968 .. 78611870
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG501700.1

Sequence Viewer

Length: 1179 bp
ATGCCTGCAGTGATCAATCTACTTGATGCAGCATGCCCATCTAAAGAAAACCCCATTACTGTTTATGTTCTCCATCTCATCGAATTAATCGGCCGAGTGACTCCCATTTTTATCTCCCACCAAGTGCAGTATAAAACAGTTTCCCATGTTAGTACATATTCAGCAAATGTCATTCTTTATTTCAATCAGTTTGCGAACGAAAATAGTGCTGCTGTTAATTTAAGCGTATTCACAGCAATCTCTCCACCAAAATTCATGCATGAAGACATATGCACGCTTGCAATGGACAAGCTCACATCCCTAATAATCCTCCCATTTCATCGAGAATGGTCGATTGATGGGTCCAGTATTGAGTCGGAAGAAGTAATAAGGACACTAAACTTCAGCGTACTCGAAACAGCTCCTTGTTCCGTTGGAATCCTTGTGGATCGGGGACATTTAGGTCGGTCCACCAGATCTGTTGTATCCACACTATCAACCTTTTCGATTGTTGTAGTCTTCTTGGGAGGGAAAGATGACAGGGTGGCATTGACCTTTGCCAAAAGAATGGCCAGGGACTCGACGATTAGGTTAACAGTAATCCATTTAGTGGCCATGGGTAATGAATATGCTGGTGGGGAATGGGAAAAAATACTTGATGCAGAGGCATTGAAGGAGTTCAATTATAACCATGTGGGCGGAGGCCTTGTAATATATTTAGAAGAGATGGAGAAGGATGGACCGCTAACGGCCTTTGTACTAAGGTCTATGGTGGATGAGTATGATCTAATCATACTTGGGAGAAGGCATAACATAGAGTCACCTCAGAGAGTAGGGCTTTCGCGATGGAGTGAATTTCAGGAGTTGGGGACAATAGGAGACTTGCTTGCATCACCTGATCTCGATTGTAAGGCATCCATTCTGATGATTCACAGAACTAAAAAGCTTCTCATTATCAACAAAGACAATGGCAGGCACAAAAGCTCATCCAGTGGTGGCTCTCGTGGCAGTGCTCATCATGGTCATCTCTCTCGCCGAGTCGCCGGCGTTGCACAAGCCCATGAAGGTCATGATCATGCTTCTGCTCCTGCGATTCCTCCAAGCACGACCTCCCCGGCCGCCTTCATCTCTCCGTCGTTCGTTTCTGCTTTCATCGCCGCCGCAGCGGCTCTCGTCTTCGGATCTGCTCTCATTGTTTGA

Protein Analysis

392

Amino Acids

42.65

Weight (kDa)

6.38

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_2nd PF23256 20 - 150 1.2e-12 Plant cation/H(+) antiporter domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AasI GACNNNNNNGTC 1 cut(s) 441
AccB7I CCANNNNNTGG 1 cut(s) 589
AccII CGCG 1 cut(s) 823
AciI CCGC 6 cut(s) 678, 722, 1098, 1137, 1140, 1145
AclWI GGATC 2 cut(s) 435, 1168
AcoI YGGCCR 4 cut(s) 91, 549, 591, 1095
AcsI RAATTY 2 cut(s) 251, 833
AcuI CTGAAG 1 cut(s) 367
AdeI CACNNNGTG 1 cut(s) 124
AfaI GTAC 3 cut(s) 154, 390, 738
AfiI CCNNNNNNNGG 1 cut(s) 589
AgsI TTSAA 3 cut(s) 184, 652, 661
AjnI CCWGG 1 cut(s) 551
AluBI AGCT 4 cut(s) 292, 401, 925, 963
AluI AGCT 4 cut(s) 292, 401, 925, 963
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 1 cut(s) 852
AlwI GGATC 2 cut(s) 435, 1168
AoxI GGCC 6 cut(s) 91, 549, 591, 682, 729, 1095
ApeKI GCWGC 3 cut(s) 29, 209, 1142
ApoI RAATTY 2 cut(s) 251, 833
AseI ATTAAT 1 cut(s) 86
Asp700I GAANNNNTTC 1 cut(s) 656
AspS9I GGNCC 3 cut(s) 342, 447, 719
AsuC2I CCSGG 1 cut(s) 1094
AsuHPI GGTGA 2 cut(s) 792, 864
AvaII GGWCC 3 cut(s) 342, 447, 719
BalI TGGCCA 2 cut(s) 551, 593
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 3 cut(s) 270, 490, 1147
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 3 cut(s) 41, 196, 1154
BccI CCATC 6 cut(s) 46, 81, 332, 700, 710, 819
BceAI ACGGC 1 cut(s) 744
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 553
BciVI GTATCC 1 cut(s) 475
BclI TGATCA 2 cut(s) 12, 1051
BcnI CCSGG 1 cut(s) 1094
BcoDI GTCTC 1 cut(s) 852
BfmI CTRYAG 1 cut(s) 6
BfuI GTATCC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 1145
BglII AGATCT 1 cut(s) 455
BisI GCNGC 7 cut(s) 30, 210, 1098, 1137, 1140, 1143, 1146
BlsI GCNGC 7 cut(s) 31, 211, 1099, 1138, 1141, 1144, 1147
Bme1390I CCNGG 2 cut(s) 553, 1094
Bme18I GGWCC 3 cut(s) 342, 447, 719
BmgT120I GGNCC 3 cut(s) 342, 447, 719
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 2 cut(s) 553, 1094
BmsI GCATC 4 cut(s) 16, 628, 878, 902
BpiI GAAGAC 3 cut(s) 270, 490, 1147
BpuMI CCSGG 1 cut(s) 1094
BsaJI CCNNGG 3 cut(s) 552, 594, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse118I RCCGGY 1 cut(s) 1022
Bse1I ACTGG 2 cut(s) 345, 969
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 552, 594, 1092
BseGI GGATG 5 cut(s) 296, 721, 760, 893, 965
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 2 cut(s) 345, 969
BseX3I CGGCCG 2 cut(s) 91, 1095
BseXI GCAGC 3 cut(s) 41, 196, 1154
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 823
Bsh1285I CGRYCG 2 cut(s) 94, 1098
BshFI GGCC 6 cut(s) 93, 551, 593, 684, 731, 1097
BsiEI CGRYCG 2 cut(s) 94, 1098
BsiHKAI GWGCWC 1 cut(s) 994
BsiSI CCGG 2 cut(s) 1023, 1094
BslFI GGGAC 3 cut(s) 447, 569, 862
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 1 cut(s) 852
BsmFI GGGAC 3 cut(s) 447, 569, 862
BsnI GGCC 6 cut(s) 93, 551, 593, 684, 731, 1097
Bsp1286I GDGCHC 1 cut(s) 994
Bsp143I GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
Bsp19I CCATGG 1 cut(s) 594
Bsp68I TCGCGA 1 cut(s) 823
BspACI CCGC 6 cut(s) 678, 722, 1098, 1137, 1140, 1145
BspANI GGCC 6 cut(s) 93, 551, 593, 684, 731, 1097
BspCNI CTCAG 1 cut(s) 817
BspFNI CGCG 1 cut(s) 823
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 435, 1168
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 1022
BsrI ACTGG 2 cut(s) 345, 969
BssAI RCCGGY 1 cut(s) 1022
BssECI CCNNGG 3 cut(s) 552, 594, 1092
BssMI GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 1 cut(s) 594
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 1 cut(s) 553
Bst4CI ACNGT 3 cut(s) 61, 139, 577
Bst6I CTCTTC 1 cut(s) 696
BstC8I GCNNGC 7 cut(s) 6, 34, 275, 279, 867, 953, 1024
BstDEI CTNAG 2 cut(s) 740, 804
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 5 cut(s) 296, 721, 760, 893, 965
BstFNI CGCG 1 cut(s) 823
BstKTI GATC 7 cut(s) 15, 430, 458, 766, 880, 1054, 1163
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
BstMCI CGRYCG 2 cut(s) 94, 1098
BstMWI GCNNNNNNNGC 5 cut(s) 984, 1028, 1133, 1142, 1145
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 1 cut(s) 36
BstSCI CCNGG 2 cut(s) 551, 1092
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 823
BstV1I GCAGC 3 cut(s) 41, 196, 1154
BstV2I GAAGAC 3 cut(s) 270, 490, 1147
BstX2I RGATCY 2 cut(s) 455, 1160
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 2 cut(s) 455, 1160
BstZI CGGCCG 2 cut(s) 91, 1095
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 6 cut(s) 93, 551, 593, 684, 731, 1097
BtgI CCRYGG 1 cut(s) 594
BtgZI GCGATG 2 cut(s) 838, 1117
BtsCI GGATG 5 cut(s) 296, 721, 760, 893, 965
BtsI GCAGTG 2 cut(s) 15, 994
BtsIMutI CAGTG 3 cut(s) 15, 976, 994
BtuMI TCGCGA 1 cut(s) 823
Cac8I GCNNGC 7 cut(s) 6, 34, 275, 279, 867, 953, 1024
CciI TCATGA 1 cut(s) 1048
Cfr10I RCCGGY 1 cut(s) 1022
Cfr13I GGNCC 3 cut(s) 342, 447, 719
Csp6I GTAC 3 cut(s) 153, 389, 737
CviQI GTAC 3 cut(s) 153, 389, 737
DdeI CTNAG 2 cut(s) 740, 804
DpnI GATC 7 cut(s) 14, 429, 457, 765, 879, 1053, 1162
DpnII GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
DraIII CACNNNGTG 1 cut(s) 124
DrdI GACNNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 441
EaeI YGGCCR 4 cut(s) 91, 549, 591, 1095
EagI CGGCCG 2 cut(s) 91, 1095
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
EciI GGCGGA 1 cut(s) 693
EclXI CGGCCG 2 cut(s) 91, 1095
Eco130I CCWWGG 1 cut(s) 594
Eco147I AGGCCT 1 cut(s) 684
Eco47I GGWCC 3 cut(s) 342, 447, 719
Eco52I CGGCCG 2 cut(s) 91, 1095
Eco57I CTGAAG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 594
FaqI GGGAC 3 cut(s) 447, 569, 862
FauNDI CATATG 1 cut(s) 269
FbaI TGATCA 2 cut(s) 12, 1051
Fnu4HI GCNGC 7 cut(s) 30, 210, 1098, 1137, 1140, 1143, 1146
FokI GGATG 5 cut(s) 283, 728, 767, 880, 952
Fsp4HI GCNGC 7 cut(s) 30, 210, 1098, 1137, 1140, 1143, 1146
GluI GCNGC 7 cut(s) 30, 210, 1098, 1137, 1140, 1143, 1146
HaeIII GGCC 6 cut(s) 93, 551, 593, 684, 731, 1097
HapII CCGG 2 cut(s) 1023, 1094
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 8 cut(s) 100, 353, 417, 557, 797, 907, 1017, 1072
HpaI GTTAAC 1 cut(s) 573
HpaII CCGG 2 cut(s) 1023, 1094
HphI GGTGA 2 cut(s) 792, 864
Hpy166II GTNNAC 2 cut(s) 450, 573
Hpy188I TCNGA 4 cut(s) 358, 807, 903, 1160
Hpy188III TCNNGA 5 cut(s) 323, 822, 839, 881, 1049
Hpy8I GTNNAC 2 cut(s) 450, 573
Hpy99I CGWCG 2 cut(s) 565, 1117
HpyAV CCTTC 5 cut(s) 646, 706, 777, 1037, 1111
HpyCH4III ACNGT 3 cut(s) 61, 139, 577
HpyCH4V TGCA 9 cut(s) 8, 29, 127, 259, 273, 281, 641, 869, 1031
HpyF10VI GCNNNNNNNGC 5 cut(s) 984, 1028, 1133, 1142, 1145
HpyF3I CTNAG 2 cut(s) 740, 804
KroI GCCGGC 1 cut(s) 1022
KroNI GCCGGC 1 cut(s) 1024
Ksp22I TGATCA 2 cut(s) 12, 1051
KspAI GTTAAC 1 cut(s) 573
Kzo9I GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
LmnI GCTCC 2 cut(s) 406, 1069
Lsp1109I GCAGC 3 cut(s) 41, 196, 1154
LweI GCATC 4 cut(s) 16, 628, 878, 902
MaeIII GTNAC 2 cut(s) 97, 798
MalI GATC 7 cut(s) 14, 429, 457, 765, 879, 1053, 1162
MboI GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
MboII GAAGA 5 cut(s) 275, 371, 490, 713, 1147
MflI RGATCY 2 cut(s) 455, 1160
MhlI GDGCHC 1 cut(s) 994
MlsI TGGCCA 2 cut(s) 551, 593
MluCI AATT 5 cut(s) 83, 217, 251, 661, 833
MluNI TGGCCA 2 cut(s) 551, 593
MlyI GAGTC 5 cut(s) 94, 362, 551, 806, 1026
MmeI TCCRAC 2 cut(s) 336, 394
MnlI CCTC 7 cut(s) 320, 500, 637, 674, 813, 1086, 1099
Mox20I TGGCCA 2 cut(s) 551, 593
Mph1103I ATGCAT 1 cut(s) 261
MreI CGCCGGCG 1 cut(s) 1022
MroNI GCCGGC 1 cut(s) 1022
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 2 cut(s) 551, 593
MseI TTAA 4 cut(s) 86, 216, 221, 572
MslI CAYNNNNRTG 2 cut(s) 902, 1053
Msp20I TGGCCA 2 cut(s) 551, 593
MspA1I CMGCKG 1 cut(s) 1145
MspI CCGG 2 cut(s) 1023, 1094
MspR9I CCNGG 2 cut(s) 553, 1094
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 5 cut(s) 984, 1028, 1133, 1142, 1145
NaeI GCCGGC 1 cut(s) 1024
NciI CCSGG 1 cut(s) 1094
NcoI CCATGG 1 cut(s) 594
NdeI CATATG 1 cut(s) 269
NdeII GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
NgoMIV GCCGGC 1 cut(s) 1022
NlaIV GGNNCC 1 cut(s) 343
NmeAIII GCCGAG 2 cut(s) 119, 1040
NmuCI GTSAC 2 cut(s) 97, 798
NruI TCGCGA 1 cut(s) 823
NsiI ATGCAT 1 cut(s) 261
NspI RCATGY 1 cut(s) 36
PaeI GCATGC 1 cut(s) 36
PagI TCATGA 1 cut(s) 1048
PceI AGGCCT 1 cut(s) 684
PdiI GCCGGC 1 cut(s) 1024
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 417, 907, 1072
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 7 cut(s) 31, 211, 1099, 1138, 1141, 1144, 1147
PleI GAGTC 5 cut(s) 94, 361, 551, 805, 1025
PpsI GAGTC 5 cut(s) 94, 361, 551, 805, 1025
PshBI ATTAAT 1 cut(s) 86
PsiI TTATAA 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 3 cut(s) 342, 447, 719
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 455, 1160
RruI TCGCGA 1 cut(s) 823
RsaI GTAC 3 cut(s) 154, 390, 738
RsaNI GTAC 3 cut(s) 153, 389, 737
RseI CAYNNNNRTG 2 cut(s) 902, 1053
SaqAI TTAA 4 cut(s) 86, 216, 221, 572
SatI GCNGC 7 cut(s) 30, 210, 1098, 1137, 1140, 1143, 1146
Sau3AI GATC 7 cut(s) 12, 427, 455, 763, 877, 1051, 1160
Sau96I GGNCC 3 cut(s) 342, 447, 719
SchI GAGTC 5 cut(s) 94, 362, 551, 806, 1026
ScrFI CCNGG 2 cut(s) 553, 1094
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 4 cut(s) 16, 628, 878, 902
SfcI CTRYAG 1 cut(s) 6
SgrAI CRCCGGYG 1 cut(s) 1022
SinI GGWCC 3 cut(s) 342, 447, 719
SmiMI CAYNNNNRTG 2 cut(s) 902, 1053
SphI GCATGC 1 cut(s) 36
Sse9I AATT 5 cut(s) 83, 217, 251, 661, 833
SseBI AGGCCT 1 cut(s) 684
SsiI CCGC 6 cut(s) 678, 722, 1098, 1137, 1140, 1145
StuI AGGCCT 1 cut(s) 684
StyD4I CCNGG 2 cut(s) 551, 1092
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 61, 139, 577
TaqI TCGA 7 cut(s) 81, 322, 332, 393, 485, 560, 882
TaqII GACCGA 1 cut(s) 435
TasI AATT 5 cut(s) 83, 217, 251, 661, 833
TatI WGTACW 2 cut(s) 152, 736
TauI GCSGC 4 cut(s) 1100, 1139, 1142, 1148
TfiI GAWTC 3 cut(s) 417, 907, 1072
Tru1I TTAA 4 cut(s) 86, 216, 221, 572
Tru9I TTAA 4 cut(s) 86, 216, 221, 572
TscAI CASTG 3 cut(s) 15, 976, 994
TseFI GTSAC 2 cut(s) 97, 798
TseI GCWGC 3 cut(s) 29, 209, 1142
Tsp45I GTSAC 2 cut(s) 97, 798
TspDTI ATGAA 7 cut(s) 244, 276, 308, 618, 1056, 1093, 1120
TspGWI ACGGA 2 cut(s) 400, 1101
TspRI CASTG 3 cut(s) 15, 976, 994
Van91I CCANNNNNTGG 1 cut(s) 589
VpaK11BI GGWCC 3 cut(s) 342, 447, 719
VspI ATTAAT 1 cut(s) 86
XapI RAATTY 2 cut(s) 251, 833
XceI RCATGY 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 656
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.