Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Forward (+)

11888056 .. 11891512

Gene Structure 3457 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00009410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGGCAGGGATGGCCAGAAACATAACATCACTGCAATCTCAGATGTGCATTGAAATACCTCCCAACGTACATTCGCAGGGCATTATAGGTCTTCGCAAAGACGGTCCGGGAATGAAGTTTAGTCTTCCGATACTGGAGATGCAGATGACTGTGATTTTCTTCATCACCCAAGCCTTCCATTGGATTTTGAAGAATTTTGAAATTCCCAAGTTCACCAGCGAGATTCTTACGGGTATACTCCTAGGCCCTTCACTCATAGGAGCCTATAATGGATTCGGGAAGGAAATCTTTCCTCTTAAGAGTCAAGAAGCAGTTGGTGTGCTGGGTGAGTTTGGTTATGGACTCTTTCTGTTTCTAATTGGAGTAAAAACGGAGCTGGAAATGATAAGAAGAACAGGGCAGAAAGCCGTGATCACCGGTATTTTATGCGTATTAGCCCCTTTGGTATTCGGTATGGTTGTCCAAAACCACCTCATGACCTCTGTGTTCGAGCTGACCAAAGATCAAATCTCTAAGCTTCCTTTTGTCACTGCAGCACATTGCATGACCCCGTTCCCCGTCGTTGTTCTCCTCCTTGAAGACCTGAATCTCCTCAATTCTGAAATCGGCCGACTAGCTCTGTCCGCATCAACAGTCAGTGACGTAGTCAGTCTCTGTCTTCGGTTTTCCTCCATTTTGCTTAGAATGGGAAGAGAAAAACCCGTGGCTGAAACTATACTAGGTTTCGGAGTAGTCGTTGGCTACATACTGGCTGTTGTGTTCATATTCAGACCAATAATGTTTTGGGTGATTAGGCAGACCCCGGAAAATAGGCCTGTCAAGAAAAGTTATATCAATATCATAATAGTAGTGGTGCTTTTTTCTGGATTACTAACACATTATTACGGCCACACTTTTCTTTTCGGGCTTGTCTTATTTGGTATGGCTGTCCCAGCTGGACGACCTTTGGGATCAGCTCTTCAAGAAAAGCTCCATGTCGTCATTTCTCAAGTGCTTCTACCCATTTATGTGACTGTATACGCAATGAGGGCAGATTTTTGGTTGGTCGATTTCAATGACCCGGCCACAGTAATCACTGCGATTCTGATCGTTTTCGTTTTTGTGGTCAAAATCATAGGCACCATGGTTGCTCCATTGTATTGTAAAATGCCCTTGAATGATGCATTTGTGCTTGCTCTTATTCTAAGCTCCAAAGGCGTTGTCCAAATTTCCATCTATACTACATTCAGAGACGATCAGACTGCAAGTGAATCGGTATATGCTTTGATTCTTGCTAGCATCGTAGTGACTGCAACTCTGGTGCCAATTATCGTGAAGCGCCTCTATAATCCTGAAAAGAAATACGCAAGCTACGAGAGAAGAGATATTATGCATTTGAAACCCAATGCTGAACTCAGAATTCTTGCCTGCATTCACAGGTCCGGTAACATTCCTGCAGTCATCAATCTAGTTGATGCAGCATGCCCATCTAAAGAAAACCCCATTGCTATTTATGTTCTTCACCTCATCGAATTAATTGGAAGAAGCTCCCCACTTTTTATCTCCCACCAATTGCAGAAAAAGAGCGATTCTAATTCTTCATACTCGGACAATGTCATTCTTTACTTCAATAACTTTATGAGACAATATCTTGGTGCTGTTACTTTAAATAAATTCACAGCAATCTCTCTACGAAAATACATGCAAGAAGACATATGCACACTTGCATCGGACAAGCTCACCTCCCTAATTGTGCTCCCGTTTCATCGAACATGGGCGATTGACGGGTCTATTGAGTCAGAAGACAATGCAATTAGGACACTAAATCGCAATGTACTCGAAAGAGCTCCTTGCTCTGTGGGCATCCTTGTGCACCGAGGCCATTTAGGCCGGTCCACCTCTGTAGTCACTTCGGGATCATCATTTTCCGTGGCTATAATCTTCCTCGGTGGGGAAGATGATAGGGAGGCATTAACATTTGCCAAAAGAATGGCCAGCGACTCAACGATTAGCTTAACTGTGATTCGATTACTCGCCATGAACAATGATTGTATTACCGGTGGGGAATGGGATAAAATACTTGAGACTGAGATATTGGAGGAGTTTAGACAGAGTCAGGTGGGTGAAGGGTGTGTGATATTTTTAGAGGAGTTGGTGAAAGATGGACCACAAACAGCATTACTACTAAGGTGTATAGTGGATGATTATGATCTAATTATAGTTGGAAGAAGGTATCAGGTAGAGTGCCCTCAGACTGCAGGACTTTCGGAATGGAGTGACTTTCCCGAGCTAGGGACTATAGGAGACCTACTTGCATCACCTGATGTAACTTGTAAAGCTTCACTTTTAGTGATAAAACATCAACAACAGATCACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

785

Amino Acids

86.97

Weight (kDa)

6.74

Isoelectric Point (pI)

44.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	62 - 441	1.3e-36	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	496 - 624	3e-15	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	634 - 780	2.3e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1118, 1300
AccI	GTMKAC	2 cut(s)	235, 1017
AciI	CCGC	1 cut(s)	624
AclWI	GGATC	2 cut(s)	958, 1903
AcoI	YGGCCR	5 cut(s)	12, 607, 886, 1062, 1971
AcsI	RAATTY	5 cut(s)	193, 201, 1206, 1398, 1652
AfaI	GTAC	2 cut(s)	69, 1815
AfiI	CCNNNNNNNGG	4 cut(s)	180, 1930, 2270, 2271
AflII	CTTAAG	1 cut(s)	296
AgeI	ACCGGT	2 cut(s)	416, 2036
AgsI	TTSAA	9 cut(s)	53, 190, 200, 578, 962, 1054, 1156, 1378, 1609
Alw21I	GWGCWC	3 cut(s)	1737, 1828, 1854
Alw26I	GTCTC	5 cut(s)	656, 1224, 1615, 2057, 2277
Alw44I	GTGCAC	1 cut(s)	1850
AlwI	GGATC	2 cut(s)	958, 1903
AlwNI	CAGNNNCTG	1 cut(s)	654
Ama87I	CYCGRG	1 cut(s)	2264
AoxI	GGCC	9 cut(s)	12, 244, 607, 812, 886, 1062, 1858, 1867, 1971
ApaLI	GTGCAC	1 cut(s)	1850
ApeKI	GCWGC	2 cut(s)	533, 1457
ApoI	RAATTY	5 cut(s)	193, 201, 1206, 1398, 1652
ArsI	GACNNNNNNTTYG	2 cut(s)	1185, 1217
AseI	ATTAAT	1 cut(s)	1514
AsiGI	ACCGGT	2 cut(s)	416, 2036
Asp700I	GAANNNNTTC	1 cut(s)	288
AspA2I	CCTAGG	1 cut(s)	241
AspLEI	GCGC	1 cut(s)	1320
AspS9I	GGNCC	5 cut(s)	104, 245, 1419, 1872, 2144
AsuC2I	CCSGG	3 cut(s)	108, 803, 1061
AsuNHI	GCTAGC	1 cut(s)	1274
AvaI	CYCGRG	1 cut(s)	2264
AvaII	GGWCC	4 cut(s)	104, 1419, 1872, 2144
AvrII	CCTAGG	1 cut(s)	241
BaeGI	GKGCMC	2 cut(s)	1854, 2228
BalI	TGGCCA	2 cut(s)	14, 1973
BanI	GGYRCC	2 cut(s)	1118, 1300
BanII	GRGCYC	1 cut(s)	1828
BbsI	GAAGAC	6 cut(s)	83, 116, 585, 650, 1695, 1788
Bbv12I	GWGCWC	3 cut(s)	1737, 1828, 1854
BbvI	GCAGC	2 cut(s)	545, 1469
BccI	CCATC	4 cut(s)	4, 1220, 1474, 2135
BceAI	ACGGC	2 cut(s)	392, 901
BcgI	CGANNNNNNTGC	8 cut(s)	1223, 1233, 1257, 1267, 1798, 1832, 2226, 2260
BclI	TGATCA	1 cut(s)	411
BcnI	CCSGG	3 cut(s)	108, 803, 1061
BcoDI	GTCTC	5 cut(s)	656, 1224, 1615, 2057, 2277
BfaI	CTAG	6 cut(s)	242, 614, 719, 1275, 1448, 2270
BfmI	CTRYAG	5 cut(s)	531, 1434, 1881, 2235, 2277
BfoI	RGCGCY	1 cut(s)	1321
BfrI	CTTAAG	1 cut(s)	296
BglI	GCCNNNNNGGC	1 cut(s)	1866
BisI	GCNGC	2 cut(s)	534, 1458
BlnI	CCTAGG	1 cut(s)	241
BlsI	GCNGC	2 cut(s)	535, 1459
Bme1390I	CCNGG	3 cut(s)	108, 803, 1061
Bme18I	GGWCC	4 cut(s)	104, 1419, 1872, 2144
BmeT110I	CYCGRG	1 cut(s)	2264
BmgT120I	GGNCC	5 cut(s)	104, 245, 1419, 1872, 2144
BmiI	GGNNCC	3 cut(s)	262, 1120, 1302
BmrFI	CCNGG	3 cut(s)	108, 803, 1061
BmsI	GCATC	8 cut(s)	129, 635, 1150, 1287, 1444, 1715, 1851, 2303
BmtI	GCTAGC	1 cut(s)	1278
BpiI	GAAGAC	6 cut(s)	83, 116, 585, 650, 1695, 1788
BpmI	CTGGAG	1 cut(s)	155
BpuEI	CTTGAG	2 cut(s)	972, 2081
BpuMI	CCSGG	3 cut(s)	108, 803, 1061
BsaBI	GATNNNNATC	2 cut(s)	1085, 2187
BsaI	GGTCTC	1 cut(s)	2277
BsaJI	CCNNGG	7 cut(s)	241, 702, 801, 1122, 1855, 1908, 1924
BsaWI	WCCGGW	3 cut(s)	416, 1421, 2036
BsaXI	ACNNNNNCTCC	2 cut(s)	720, 750
Bsc4I	CCNNNNNNNGG	4 cut(s)	180, 1930, 2270, 2271
Bse118I	RCCGGY	3 cut(s)	416, 1869, 2036
Bse1I	ACTGG	2 cut(s)	138, 753
Bse3DI	GCAATG	4 cut(s)	538, 1029, 1482, 1816
Bse8I	GATNNNNATC	2 cut(s)	1085, 2187
BseDI	CCNNGG	7 cut(s)	241, 702, 801, 1122, 1855, 1908, 1924
BseGI	GGATG	3 cut(s)	15, 1842, 2185
BseJI	GATNNNNATC	2 cut(s)	1085, 2187
BseLI	CCNNNNNNNGG	4 cut(s)	180, 1930, 2270, 2271
BseMI	GCAATG	4 cut(s)	538, 1029, 1482, 1816
BseMII	CTCAG	4 cut(s)	53, 1408, 2058, 2243
BseNI	ACTGG	2 cut(s)	138, 753
BseRI	GAGGAG	4 cut(s)	560, 581, 2093, 2141
BseSI	GKGCMC	2 cut(s)	1854, 2228
BseX3I	CGGCCG	1 cut(s)	607
BseXI	GCAGC	2 cut(s)	545, 1469
BseYI	CCCAGC	2 cut(s)	322, 931
Bsh1285I	CGRYCG	1 cut(s)	610
BshFI	GGCC	9 cut(s)	14, 246, 609, 814, 888, 1064, 1860, 1869, 1973
BshNI	GGYRCC	2 cut(s)	1118, 1300
BshTI	ACCGGT	2 cut(s)	416, 2036
BsiEI	CGRYCG	1 cut(s)	610
BsiHKAI	GWGCWC	3 cut(s)	1737, 1828, 1854
BsiHKCI	CYCGRG	1 cut(s)	2264
BsiSI	CCGG	7 cut(s)	107, 417, 803, 1061, 1422, 1870, 2037
BslFI	GGGAC	2 cut(s)	914, 2287
BslI	CCNNNNNNNGG	4 cut(s)	180, 1930, 2270, 2271
BsmAI	GTCTC	5 cut(s)	656, 1224, 1615, 2057, 2277
BsmBI	CGTCTC	1 cut(s)	1224
BsmFI	GGGAC	2 cut(s)	914, 2287
BsmI	GAATGC	1 cut(s)	1410
BsnI	GGCC	9 cut(s)	14, 246, 609, 814, 888, 1064, 1860, 1869, 1973
Bso31I	GGTCTC	1 cut(s)	2277
BsoBI	CYCGRG	1 cut(s)	2264
Bsp1286I	GDGCHC	4 cut(s)	1737, 1828, 1854, 2228
Bsp143I	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
Bsp19I	CCATGG	1 cut(s)	1122
BspACI	CCGC	1 cut(s)	624
BspANI	GGCC	9 cut(s)	14, 246, 609, 814, 888, 1064, 1860, 1869, 1973
BspCNI	CTCAG	4 cut(s)	52, 1407, 2059, 2242
BspHI	TCATGA	1 cut(s)	474
BspLI	GGNNCC	3 cut(s)	262, 1120, 1302
BspMAI	CTGCAG	3 cut(s)	535, 1438, 2239
BspOI	GCTAGC	1 cut(s)	1278
BspPI	GGATC	2 cut(s)	958, 1903
BspQI	GCTCTTC	1 cut(s)	963
BspT107I	GGYRCC	2 cut(s)	1118, 1300
BspTI	CTTAAG	1 cut(s)	296
BspTNI	GGTCTC	1 cut(s)	2277
BsrDI	GCAATG	4 cut(s)	538, 1029, 1482, 1816
BsrFI	RCCGGY	3 cut(s)	416, 1869, 2036
BsrI	ACTGG	2 cut(s)	138, 753
BssAI	RCCGGY	3 cut(s)	416, 1869, 2036
BssECI	CCNNGG	7 cut(s)	241, 702, 801, 1122, 1855, 1908, 1924
BssMI	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
BssNAI	GTATAC	2 cut(s)	236, 1018
BssT1I	CCWWGG	2 cut(s)	241, 1122
Bst1107I	GTATAC	2 cut(s)	236, 1018
Bst4CI	ACNGT	6 cut(s)	104, 151, 634, 1015, 1069, 1999
Bst6I	CTCTTC	3 cut(s)	685, 963, 1354
BstAFI	CTTAAG	1 cut(s)	296
BstC8I	GCNNGC	6 cut(s)	1173, 1276, 1348, 1408, 1462, 1975
BstDEI	CTNAG	8 cut(s)	39, 513, 680, 1184, 1394, 2067, 2165, 2229
BstDSI	CCRYGG	3 cut(s)	702, 1122, 1908
BstF5I	GGATG	3 cut(s)	15, 1842, 2185
BstH2I	RGCGCY	1 cut(s)	1321
BstHHI	GCGC	1 cut(s)	1320
BstKTI	GATC	8 cut(s)	414, 505, 953, 1089, 1237, 1898, 2191, 2352
BstMAI	GTCTC	5 cut(s)	656, 1224, 1615, 2057, 2277
BstMBI	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
BstMCI	CGRYCG	1 cut(s)	610
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 623, 932, 1028, 1194, 1839, 1866
BstNSI	RCATGY	2 cut(s)	1464, 1684
BstSCI	CCNGG	3 cut(s)	106, 801, 1059
BstSFI	CTRYAG	5 cut(s)	531, 1434, 1881, 2235, 2277
BstSLI	GKGCMC	2 cut(s)	1854, 2228
BstV1I	GCAGC	2 cut(s)	545, 1469
BstV2I	GAAGAC	6 cut(s)	83, 116, 585, 650, 1695, 1788
BstXI	CCANNNNNNTGG	1 cut(s)	1969
BstZ17I	GTATAC	2 cut(s)	236, 1018
BstZI	CGGCCG	1 cut(s)	607
BsuRI	GGCC	9 cut(s)	14, 246, 609, 814, 888, 1064, 1860, 1869, 1973
BtgI	CCRYGG	3 cut(s)	702, 1122, 1908
BtsCI	GGATG	3 cut(s)	15, 1842, 2185
BtsI	GCAGTG	3 cut(s)	29, 528, 1074
BtsIMutI	CAGTG	4 cut(s)	29, 528, 643, 1074
Cac8I	GCNNGC	6 cut(s)	1173, 1276, 1348, 1408, 1462, 1975
CaiI	CAGNNNCTG	1 cut(s)	654
CciI	TCATGA	1 cut(s)	474
CfoI	GCGC	1 cut(s)	1320
Cfr10I	RCCGGY	3 cut(s)	416, 1869, 2036
Cfr13I	GGNCC	5 cut(s)	104, 245, 1419, 1872, 2144
CpoI	CGGWCCG	1 cut(s)	104
Csp6I	GTAC	2 cut(s)	68, 1814
CspAI	ACCGGT	2 cut(s)	416, 2036
CspI	CGGWCCG	1 cut(s)	104
CviAII	CATG	8 cut(s)	475, 544, 974, 1123, 1461, 1681, 1752, 2017
CviQI	GTAC	2 cut(s)	68, 1814
DdeI	CTNAG	8 cut(s)	39, 513, 680, 1184, 1394, 2067, 2165, 2229
DpnI	GATC	8 cut(s)	413, 504, 952, 1088, 1236, 1897, 2190, 2351
DpnII	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
DraI	TTTAAA	1 cut(s)	1647
EaeI	YGGCCR	5 cut(s)	12, 607, 886, 1062, 1971
EagI	CGGCCG	1 cut(s)	607
Eam1104I	CTCTTC	3 cut(s)	685, 963, 1354
EarI	CTCTTC	3 cut(s)	685, 963, 1354
Ecl136II	GAGCTC	1 cut(s)	1826
EclXI	CGGCCG	1 cut(s)	607
Eco130I	CCWWGG	2 cut(s)	241, 1122
Eco147I	AGGCCT	1 cut(s)	814
Eco24I	GRGCYC	1 cut(s)	1828
Eco31I	GGTCTC	1 cut(s)	2277
Eco47I	GGWCC	4 cut(s)	104, 1419, 1872, 2144
Eco52I	CGGCCG	1 cut(s)	607
Eco53kI	GAGCTC	1 cut(s)	1826
Eco88I	CYCGRG	1 cut(s)	2264
EcoICRI	GAGCTC	1 cut(s)	1826
EcoO109I	RGGNCCY	1 cut(s)	245
EcoRI	GAATTC	1 cut(s)	1398
EcoT14I	CCWWGG	2 cut(s)	241, 1122
EcoT22I	ATGCAT	2 cut(s)	1165, 1374
EcoT38I	GRGCYC	1 cut(s)	1828
ErhI	CCWWGG	2 cut(s)	241, 1122
Esp3I	CGTCTC	1 cut(s)	1224
FaeI	CATG	8 cut(s)	478, 547, 977, 1126, 1464, 1684, 1755, 2020
FalI	AAGNNNNNCTT	6 cut(s)	272, 304, 1813, 1845, 2307, 2339
FaqI	GGGAC	2 cut(s)	914, 2287
FatI	CATG	8 cut(s)	474, 543, 973, 1122, 1460, 1680, 1751, 2016
FauNDI	CATATG	1 cut(s)	1694
FbaI	TGATCA	1 cut(s)	411
FblI	GTMKAC	2 cut(s)	235, 1017
Fnu4HI	GCNGC	2 cut(s)	534, 1458
FokI	GGATG	3 cut(s)	22, 1829, 2192
FriOI	GRGCYC	1 cut(s)	1828
Fsp4HI	GCNGC	2 cut(s)	534, 1458
FspBI	CTAG	6 cut(s)	242, 614, 719, 1275, 1448, 2270
GlaI	GCGC	1 cut(s)	1319
GluI	GCNGC	2 cut(s)	534, 1458
GsaI	CCCAGC	2 cut(s)	326, 935
GsuI	CTGGAG	1 cut(s)	155
HaeII	RGCGCY	1 cut(s)	1321
HaeIII	GGCC	9 cut(s)	14, 246, 609, 814, 888, 1064, 1860, 1869, 1973
HapII	CCGG	7 cut(s)	107, 417, 803, 1061, 1422, 1870, 2037
HhaI	GCGC	1 cut(s)	1320
Hin1II	CATG	8 cut(s)	478, 547, 977, 1126, 1464, 1684, 1755, 2020
Hin6I	GCGC	1 cut(s)	1318
HinP1I	GCGC	1 cut(s)	1318
HindIII	AAGCTT	2 cut(s)	515, 2316
HpaII	CCGG	7 cut(s)	107, 417, 803, 1061, 1422, 1870, 2037
Hpy166II	GTNNAC	5 cut(s)	213, 236, 1018, 1852, 1875
Hpy8I	GTNNAC	5 cut(s)	213, 236, 1018, 1852, 1875
Hpy99I	CGWCG	1 cut(s)	563
HpyAV	CCTTC	5 cut(s)	184, 258, 274, 2099, 2202
HpyCH4III	ACNGT	6 cut(s)	104, 151, 634, 1015, 1069, 1999
HpyCH4IV	ACGT	2 cut(s)	66, 642
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 623, 932, 1028, 1194, 1839, 1866
HpyF3I	CTNAG	8 cut(s)	39, 513, 680, 1184, 1394, 2067, 2165, 2229
HpySE526I	ACGT	2 cut(s)	66, 642
Hsp92II	CATG	8 cut(s)	478, 547, 977, 1126, 1464, 1684, 1755, 2020
HspAI	GCGC	1 cut(s)	1318
Ksp22I	TGATCA	1 cut(s)	411
Kzo9I	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	8 cut(s)	260, 373, 975, 1135, 1193, 1532, 1740, 1831
Lsp1109I	GCAGC	2 cut(s)	545, 1469
LweI	GCATC	8 cut(s)	129, 635, 1150, 1287, 1444, 1715, 1851, 2303
MaeI	CTAG	6 cut(s)	242, 614, 719, 1275, 1448, 2270
MaeII	ACGT	2 cut(s)	66, 642
MaeIII	GTNAC	9 cut(s)	526, 638, 1009, 1285, 1424, 1639, 1885, 2255, 2305
MalI	GATC	8 cut(s)	413, 504, 952, 1088, 1236, 1897, 2190, 2351
MboI	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
MfeI	CAATTG	1 cut(s)	1550
MhlI	GDGCHC	4 cut(s)	1737, 1828, 1854, 2228
MlsI	TGGCCA	2 cut(s)	14, 1973
MluNI	TGGCCA	2 cut(s)	14, 1973
MlyI	GAGTC	5 cut(s)	310, 336, 1784, 1973, 2101
MmeI	TCCRAC	1 cut(s)	2182
Mox20I	TGGCCA	2 cut(s)	14, 1973
Mph1103I	ATGCAT	2 cut(s)	1165, 1374
MroXI	GAANNNNTTC	1 cut(s)	288
MscI	TGGCCA	2 cut(s)	14, 1973
MseI	TTAA	6 cut(s)	297, 1514, 1646, 1952, 1994, 2356
MslI	CAYNNNNRTG	3 cut(s)	1007, 1283, 1847
Msp20I	TGGCCA	2 cut(s)	14, 1973
MspA1I	CMGCKG	1 cut(s)	935
MspCI	CTTAAG	1 cut(s)	296
MspI	CCGG	7 cut(s)	107, 417, 803, 1061, 1422, 1870, 2037
MspR9I	CCNGG	3 cut(s)	108, 803, 1061
MunI	CAATTG	1 cut(s)	1550
Mva1269I	GAATGC	1 cut(s)	1410
MwoI	GCNNNNNNNGC	7 cut(s)	11, 623, 932, 1028, 1194, 1839, 1866
NciI	CCSGG	3 cut(s)	108, 803, 1061
NcoI	CCATGG	1 cut(s)	1122
NdeI	CATATG	1 cut(s)	1694
NdeII	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
NheI	GCTAGC	1 cut(s)	1274
NlaIII	CATG	8 cut(s)	478, 547, 977, 1126, 1464, 1684, 1755, 2020
NlaIV	GGNNCC	3 cut(s)	262, 1120, 1302
NmuCI	GTSAC	6 cut(s)	526, 638, 1009, 1285, 1885, 2255
NsiI	ATGCAT	2 cut(s)	1165, 1374
NspI	RCATGY	2 cut(s)	1464, 1684
PaeI	GCATGC	1 cut(s)	1464
PagI	TCATGA	1 cut(s)	474
PceI	AGGCCT	1 cut(s)	814
PciSI	GCTCTTC	1 cut(s)	963
PcsI	WCGNNNNNNNCGW	2 cut(s)	732, 1350
PctI	GAATGC	1 cut(s)	1410
PdmI	GAANNNNTTC	1 cut(s)	288
PfeI	GAWTC	8 cut(s)	223, 273, 586, 1081, 1250, 1267, 1568, 2002
PflFI	GACNNNGTC	3 cut(s)	644, 1592, 2091
PfoI	TCCNGGA	1 cut(s)	106
PinAI	ACCGGT	2 cut(s)	416, 2036
PkrI	GCNGC	2 cut(s)	535, 1459
PleI	GAGTC	5 cut(s)	309, 336, 1783, 1973, 2100
PpsI	GAGTC	5 cut(s)	309, 336, 1783, 1973, 2100
PshBI	ATTAAT	1 cut(s)	1514
Psp124BI	GAGCTC	1 cut(s)	1828
PspFI	CCCAGC	2 cut(s)	322, 931
PspN4I	GGNNCC	3 cut(s)	262, 1120, 1302
PspPI	GGNCC	5 cut(s)	104, 245, 1419, 1872, 2144
PstI	CTGCAG	3 cut(s)	535, 1438, 2239
PstNI	CAGNNNCTG	1 cut(s)	654
PsyI	GACNNNGTC	3 cut(s)	644, 1592, 2091
PvuII	CAGCTG	1 cut(s)	935
RsaI	GTAC	2 cut(s)	69, 1815
RsaNI	GTAC	2 cut(s)	68, 1814
RseI	CAYNNNNRTG	3 cut(s)	1007, 1283, 1847
Rsr2I	CGGWCCG	1 cut(s)	104
RsrII	CGGWCCG	1 cut(s)	104
SacI	GAGCTC	1 cut(s)	1828
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	6 cut(s)	297, 1514, 1646, 1952, 1994, 2356
SatI	GCNGC	2 cut(s)	534, 1458
Sau3AI	GATC	8 cut(s)	411, 502, 950, 1086, 1234, 1895, 2188, 2349
Sau96I	GGNCC	5 cut(s)	104, 245, 1419, 1872, 2144
SchI	GAGTC	5 cut(s)	310, 336, 1784, 1973, 2101
ScrFI	CCNGG	3 cut(s)	108, 803, 1061
SduI	GDGCHC	4 cut(s)	1737, 1828, 1854, 2228
SfaNI	GCATC	8 cut(s)	129, 635, 1150, 1287, 1444, 1715, 1851, 2303
SfcI	CTRYAG	5 cut(s)	531, 1434, 1881, 2235, 2277
SfiI	GGCCNNNNNGGCC	1 cut(s)	1866
SinI	GGWCC	4 cut(s)	104, 1419, 1872, 2144
SmiMI	CAYNNNNRTG	3 cut(s)	1007, 1283, 1847
SmlI	CTYRAG	3 cut(s)	296, 987, 2060
SmoI	CTYRAG	3 cut(s)	296, 987, 2060
SphI	GCATGC	1 cut(s)	1464
SseBI	AGGCCT	1 cut(s)	814
SsiI	CCGC	1 cut(s)	624
SspMI	CTAG	6 cut(s)	242, 614, 719, 1275, 1448, 2270
SstI	GAGCTC	1 cut(s)	1828
StuI	AGGCCT	1 cut(s)	814
StyD4I	CCNGG	3 cut(s)	106, 801, 1059
StyI	CCWWGG	2 cut(s)	241, 1122
TaaI	ACNGT	6 cut(s)	104, 151, 634, 1015, 1069, 1999
TaiI	ACGT	2 cut(s)	69, 645
TaqI	TCGA	6 cut(s)	489, 1047, 1509, 1747, 1818, 2005
TatI	WGTACW	1 cut(s)	1813
TfiI	GAWTC	8 cut(s)	223, 273, 586, 1081, 1250, 1267, 1568, 2002
Tru1I	TTAA	6 cut(s)	297, 1514, 1646, 1952, 1994, 2356
Tru9I	TTAA	6 cut(s)	297, 1514, 1646, 1952, 1994, 2356
TscAI	CASTG	4 cut(s)	36, 535, 643, 1081
TseFI	GTSAC	6 cut(s)	526, 638, 1009, 1285, 1885, 2255
TseI	GCWGC	2 cut(s)	533, 1457
Tsp45I	GTSAC	6 cut(s)	526, 638, 1009, 1285, 1885, 2255
TspDTI	ATGAA	6 cut(s)	128, 151, 751, 1569, 1733, 2033
TspGWI	ACGGA	2 cut(s)	386, 1897
TspRI	CASTG	4 cut(s)	36, 535, 643, 1081
Tth111I	GACNNNGTC	3 cut(s)	644, 1592, 2091
Vha464I	CTTAAG	1 cut(s)	296
VneI	GTGCAC	1 cut(s)	1850
VpaK11BI	GGWCC	4 cut(s)	104, 1419, 1872, 2144
VspI	ATTAAT	1 cut(s)	1514
XapI	RAATTY	5 cut(s)	193, 201, 1206, 1398, 1652
XceI	RCATGY	2 cut(s)	1464, 1684
XcmI	CCANNNNNNNNNTGG	1 cut(s)	780
XmaJI	CCTAGG	1 cut(s)	241
XmiI	GTMKAC	2 cut(s)	235, 1017
XmnI	GAANNNNTTC	1 cut(s)	288
XspI	CTAG	6 cut(s)	242, 614, 719, 1275, 1448, 2270
Zsp2I	ATGCAT	2 cut(s)	1165, 1374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.