Rroxscaffold_5G00386970

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001192
Physical Location & Seq
Reverse (-)
65692143 .. 65694748
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386970.1

Sequence Viewer

Length: 2286 bp
ATGAGTATTACACCGGCGTCGTCCCTGTTCAATAAGAGCTTGACTTGCGTGGAGTTACCGCCCAACATTCATTCAAGTGGTATGTGGCAGAAACTAGATACTACTGCTGCAGATGTTGAGTTTGAGACCACGAGTGCTACATTGCCAATGCTCCATAAGAAAATGCTTATGACCTTCTTAGCCACCCAGCTCTTCCATCTCATTCTCGGTTCTTTTGGCGTCCCATTCTTCGTCTCTCAAATGCTGGCAGGATGCTTACTTGGTCCAGCGGTGGTTGGCAGAATTAAATTCTACGAAAATTGGTACAATGACTGGATGTTGTCCTTGACAAGTCAGGACATACTTGGCACTCTCACAATGTTTGGCTTCTCCATGTTTTTATTTCTGGCAGCAGTGAAGATGGATGCAAGCCTGTTATTGAAGACTGGGAGAAAGGCCTTTTACACTGGTGTATTCTCCTTGATAGTGCCCCTAATTGTTGGCTTTTCCACAGTTGGGTTGCTTGAATATCAGAAGGCGAAAATTAATCCGAAAGAGAAAAAGAACCAACTCCGTATCCTTACTTCTGAACTCGGGCGATTAGGATTGTCAGCAGCTCTAGTGTGTGACATGCTAAACTTGATTCTCACTACTATTGGGAGAGCGTTTGTTCCAACCATGGGTGCCAGAAGGTTTAGTTACAAGTCGTCTCTTGTTATCTTGGGATATAGTCTGGCTTGTGCGTTCGTGGCCCGGCCGGCTTTGAATTGGGTCATCAGACAGACACCTAAAGGGAAGCCTGTTAACAATGGCTACATTTTTGTCATCCTCCTGCTGGCCTTGGCCTCCGGAGCTCATGAGCAACTTGTTTTATTTGGACCTTTCTTTTTCGGCTTAGTTATACCAAGTGGACCTCCCCTCGGGTCGACCCTGGTTCAGAAATACGACTTCATGGTCTCGCGTGTGTTCATGCCACTGTTTGTCACCAATTGTGTGATCATGGCAGATGTCAGGGAACTCCGCTTCGATGTCCATTTACTAGTCTTGCTGGTAACTATGTTGGCCAAATTTTTGGGCACTTTAGCACCTGCACTCTGCTGTAGAATTCCTATCAGGGATGCTTTGGCACTCGCTTTCATACTGAGCTGCAAAGGTGCTGTTGAAATCGGTGGTTACTTCGTGATCTATGACGGCCAGCGTATCACCGACGGAATTTATGCTTTGGCGATGTTGATGATCCTATTCGCCCATTTCACCGTGGCATATGCTGTGAAGCACTTGTACAAGACATCGACAAGGTACGCAGGGTATCAGCAGAGAAACGTACTAGGCATGAGACCTAACTCGGAGCTCAAGATACTTTCCTGCATCTACAAACACGACAACGTGCCAGCTGTCATCAACTTATTGGATGTGGCTTGCCCTACCGGAGATAACCCCATTGGTCTCAACGTCCTCCACCTTATAGAGATGACCGGCCAAGACGCCCCCATGCTCATTTCCCACCGCATGCAGAAGAAGACAATGTCCAGCTTCTCCGACTCAGAGGACGTCCTCGTTTCTTTCCTTAATTTCGAGCGGGAGTACGGTGACGCCGTTGAGGTCCACCCCTATACCGCCGTCTGGCCGTCAAAGTGGATGTACGAAGAGATATGCACACTCGCCCTAGACAGGCTCACACATCTCATCATACTCCCCTTCCACCGCCAGTGGGCCGTGGACGGCGCTGTTAAATCGGAGGACGAAACCGTCAGGAACATAAACATTAGCGTTCTAGATGGATCGCCTTGTTCCGTCGGAGTTCTAGTCAACCGGGGCCCGCTCCGGCAAAACCACAGAACAGAAGCGTCGCAATCACAGTTCAACGTTGCGTTGATTTTCTTGGGTGGCGAAGACGATAGGGAGGCGTTGGCTTTGACGGGAAGGATGACCGCCGACCCAAACATCACCCTAACTGTGTTTCATATCAGTCCCACCACACTGCCTGAAAATGGACCTAACTGGGAGAACATTCTGGACTTTGAGAGCTTAAGGAAGTTCAGGCAAAACAACACCGGCAATGGCCGTTACGTGATTTACACGGAGGCGGTTTCGGAGAGTGGGGAACAAACTGTTGACATGTTGAGGGAGATCGTGCACAAGTATGACCTTTTCGTGGTTGGGAGGAGGAAAGAGGTTAAGGATAGTCCTCAGACTTCAGGGCTTGAAGATTGGAGTGAGTTCCCGGAGCTTGGGATTGTAGGTGACATGCTCGCATCGGATGATTTGGGTTGCAGATCTTCTATCTTGGTGGTGCAGAACCAATGA

Protein Analysis

761

Amino Acids

84.6

Weight (kDa)

6.77

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 68 - 165 2.5e-09 Sodium/hydrogen exchanger, transmembrane
Na_H_Exchanger PF00999 185 - 411 8.6e-12 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 478 - 599 1.1e-07 Plant cation/H(+) antiporter domain
CHX17_C PF23259 615 - 760 9.2e-09 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1075
AasI GACNNNNNNGTC 2 cut(s) 932, 1727
AatII GACGTC 1 cut(s) 1533
Acc36I ACCTGC 1 cut(s) 1075
AccB1I GGYRCC 1 cut(s) 662
AccBSI CCGCTC 2 cut(s) 1558, 1801
AccI GTMKAC 1 cut(s) 905
AccII CGCG 1 cut(s) 940
AccIII TCCGGA 1 cut(s) 827
AclI AACGTT 1 cut(s) 1845
AclWI GGATC 2 cut(s) 1210, 1768
AcoI YGGCCR 6 cut(s) 734, 1041, 1171, 1456, 1604, 2041
AcsI RAATTY 4 cut(s) 287, 1046, 1083, 1191
AcuI CTGAAG 1 cut(s) 2160
AcyI GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
AfaI GTAC 6 cut(s) 305, 1262, 1280, 1305, 1565, 1622
AflII CTTAAG 1 cut(s) 2008
AflIII ACRYGT 1 cut(s) 2097
AgsI TTSAA 8 cut(s) 31, 75, 421, 506, 745, 1142, 1843, 2186
AhlI ACTAGT 1 cut(s) 1018
AjnI CCWGG 1 cut(s) 909
Alw21I GWGCWC 3 cut(s) 835, 1332, 2118
Alw26I GTCTC 6 cut(s) 119, 238, 693, 940, 1309, 1430
Alw44I GTGCAC 1 cut(s) 2114
AlwI GGATC 2 cut(s) 1210, 1768
Ama87I CYCGRG 2 cut(s) 572, 899
Aor13HI TCCGGA 1 cut(s) 827
ApaI GGGCCC 1 cut(s) 1799
ApaLI GTGCAC 1 cut(s) 2114
ApeKI GCWGC 4 cut(s) 107, 389, 593, 1125
ApoI RAATTY 4 cut(s) 287, 1046, 1083, 1191
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 1 cut(s) 1706
AspS9I GGNCC 9 cut(s) 263, 730, 857, 890, 1582, 1692, 1795, 1796, 1973
AsuC2I CCSGG 3 cut(s) 733, 1793, 2204
AsuHPI GGTGA 6 cut(s) 955, 1174, 1225, 1580, 1918, 2234
AvaI CYCGRG 2 cut(s) 572, 899
AvaII GGWCC 5 cut(s) 263, 857, 890, 1582, 1973
BaeGI GKGCMC 4 cut(s) 471, 1058, 1799, 2118
BalI TGGCCA 1 cut(s) 1043
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 3 cut(s) 835, 1332, 1799
BarI GAAGNNNNNNTAC 4 cut(s) 547, 579, 1244, 1276
BauI CACGAG 1 cut(s) 130
BbsI GAAGAC 3 cut(s) 428, 1505, 1878
Bbv12I GWGCWC 3 cut(s) 835, 1332, 2118
BbvI GCAGC 4 cut(s) 94, 401, 605, 1112
BccI CCATC 3 cut(s) 204, 394, 1751
BceAI ACGGC 7 cut(s) 1186, 1559, 1583, 1591, 1679, 1717, 2028
BciT130I CCWGG 1 cut(s) 911
BciVI GTATCC 1 cut(s) 566
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 3 cut(s) 733, 1793, 2204
BcoDI GTCTC 6 cut(s) 119, 238, 693, 940, 1309, 1430
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 7 cut(s) 95, 599, 1019, 1307, 1646, 1754, 1784
BfmI CTRYAG 2 cut(s) 108, 1078
BfoI RGCGCY 1 cut(s) 1707
BfrI CTTAAG 1 cut(s) 2008
BfuAI ACCTGC 1 cut(s) 1075
BfuI GTATCC 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 737
BglII AGATCT 1 cut(s) 2255
BisI GCNGC 4 cut(s) 108, 390, 594, 1126
BlsI GCNGC 4 cut(s) 109, 391, 595, 1127
Bme1390I CCNGG 4 cut(s) 733, 911, 1793, 2204
Bme18I GGWCC 5 cut(s) 263, 857, 890, 1582, 1973
BmeT110I CYCGRG 2 cut(s) 572, 899
BmgT120I GGNCC 9 cut(s) 263, 730, 857, 890, 1582, 1692, 1795, 1796, 1973
BmiI GGNNCC 3 cut(s) 664, 1796, 1797
BmrFI CCNGG 4 cut(s) 733, 911, 1793, 2204
BmrI ACTGGG 2 cut(s) 435, 1990
BmsI GCATC 5 cut(s) 242, 394, 1087, 1356, 2243
BmuI ACTGGG 2 cut(s) 435, 1990
BpiI GAAGAC 3 cut(s) 428, 1505, 1878
BpuEI CTTGAG 1 cut(s) 1316
BpuMI CCSGG 3 cut(s) 733, 1793, 2204
BsaAI YACGTR 1 cut(s) 2050
BsaHI GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
BsaI GGTCTC 4 cut(s) 119, 940, 1309, 1430
BsaJI CCNNGG 7 cut(s) 657, 819, 898, 909, 1236, 1695, 1792
BsaWI WCCGGW 2 cut(s) 827, 1406
BsaXI ACNNNNNCTCC 2 cut(s) 1770, 1800
Bse118I RCCGGY 4 cut(s) 13, 736, 1454, 2033
Bse1I ACTGG 5 cut(s) 317, 430, 451, 1687, 1985
Bse3DI GCAATG 2 cut(s) 140, 2044
BseAI TCCGGA 1 cut(s) 827
BseBI CCWGG 1 cut(s) 911
BseDI CCNNGG 7 cut(s) 657, 819, 898, 909, 1236, 1695, 1792
BseGI GGATG 9 cut(s) 257, 321, 409, 804, 1102, 1396, 1623, 1911, 2245
BseMI GCAATG 2 cut(s) 140, 2044
BseMII CTCAG 3 cut(s) 1112, 1536, 2183
BseNI ACTGG 5 cut(s) 317, 430, 451, 1687, 1985
BseRI GAGGAG 1 cut(s) 2158
BseSI GKGCMC 4 cut(s) 471, 1058, 1799, 2118
BseX3I CGGCCG 1 cut(s) 734
BseXI GCAGC 4 cut(s) 94, 401, 605, 1112
BseYI CCCAGC 1 cut(s) 186
BsgI GTGCAG 1 cut(s) 1053
Bsh1236I CGCG 1 cut(s) 940
Bsh1285I CGRYCG 1 cut(s) 737
BshNI GGYRCC 1 cut(s) 662
BsiEI CGRYCG 1 cut(s) 737
BsiHKAI GWGCWC 3 cut(s) 835, 1332, 2118
BsiHKCI CYCGRG 2 cut(s) 572, 899
BslFI GGGAC 3 cut(s) 7, 206, 1935
BsmAI GTCTC 6 cut(s) 119, 238, 693, 940, 1309, 1430
BsmBI CGTCTC 2 cut(s) 238, 693
BsmFI GGGAC 3 cut(s) 7, 206, 1935
Bso31I GGTCTC 4 cut(s) 119, 940, 1309, 1430
BsoBI CYCGRG 2 cut(s) 572, 899
Bsp120I GGGCCC 1 cut(s) 1795
Bsp1286I GDGCHC 6 cut(s) 471, 835, 1058, 1332, 1799, 2118
Bsp13I TCCGGA 1 cut(s) 827
Bsp1407I TGTACA 1 cut(s) 1260
Bsp143I GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
Bsp19I CCATGG 1 cut(s) 657
BspCNI CTCAG 3 cut(s) 1113, 1535, 2182
BspEI TCCGGA 1 cut(s) 827
BspFNI CGCG 1 cut(s) 940
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 3 cut(s) 664, 1796, 1797
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 2 cut(s) 1210, 1768
BspQI GCTCTTC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 662
BspTI CTTAAG 1 cut(s) 2008
BspTNI GGTCTC 4 cut(s) 119, 940, 1309, 1430
BsrBI CCGCTC 2 cut(s) 1558, 1801
BsrDI GCAATG 2 cut(s) 140, 2044
BsrFI RCCGGY 4 cut(s) 13, 736, 1454, 2033
BsrGI TGTACA 1 cut(s) 1260
BsrI ACTGG 5 cut(s) 317, 430, 451, 1687, 1985
BssAI RCCGGY 4 cut(s) 13, 736, 1454, 2033
BssECI CCNNGG 7 cut(s) 657, 819, 898, 909, 1236, 1695, 1792
BssMI GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
BssNI GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 657, 819
Bst2BI CACGAG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 8 cut(s) 493, 957, 1237, 1568, 1729, 1839, 1936, 2092
Bst6I CTCTTC 2 cut(s) 197, 1620
BstACI GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
BstAFI CTTAAG 1 cut(s) 2008
BstAUI TGTACA 1 cut(s) 1260
BstBAI YACGTR 1 cut(s) 2050
BstDEI CTNAG 5 cut(s) 178, 874, 1121, 1522, 2169
BstDSI CCRYGG 3 cut(s) 657, 1236, 1695
BstF5I GGATG 9 cut(s) 257, 321, 409, 804, 1102, 1396, 1623, 1911, 2245
BstFNI CGCG 1 cut(s) 940
BstH2I RGCGCY 1 cut(s) 1707
BstHHI GCGC 1 cut(s) 1706
BstKTI GATC 6 cut(s) 978, 1164, 1218, 1763, 2112, 2258
BstMAI GTCTC 6 cut(s) 119, 238, 693, 940, 1309, 1430
BstMBI GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
BstMCI CGRYCG 1 cut(s) 737
BstMWI GCNNNNNNNGC 4 cut(s) 45, 728, 737, 830
BstNI CCWGG 1 cut(s) 911
BstNSI RCATGY 4 cut(s) 613, 1492, 2101, 2230
BstSCI CCNGG 4 cut(s) 731, 909, 1791, 2202
BstSFI CTRYAG 2 cut(s) 108, 1078
BstSLI GKGCMC 4 cut(s) 471, 1058, 1799, 2118
BstUI CGCG 1 cut(s) 940
BstV1I GCAGC 4 cut(s) 94, 401, 605, 1112
BstV2I GAAGAC 3 cut(s) 428, 1505, 1878
BstX2I RGATCY 1 cut(s) 2255
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 2255
BstZI CGGCCG 1 cut(s) 734
BsuI GTATCC 1 cut(s) 566
BtgI CCRYGG 3 cut(s) 657, 1236, 1695
BtgZI GCGATG 1 cut(s) 1220
BtsCI GGATG 9 cut(s) 257, 321, 409, 804, 1102, 1396, 1623, 1911, 2245
BtsI GCAGTG 2 cut(s) 399, 1958
BtsIMutI CAGTG 5 cut(s) 399, 444, 953, 1694, 1958
BveI ACCTGC 1 cut(s) 1075
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 1706
Cfr10I RCCGGY 4 cut(s) 13, 736, 1454, 2033
Cfr13I GGNCC 9 cut(s) 263, 730, 857, 890, 1582, 1692, 1795, 1796, 1973
CseI GACGC 5 cut(s) 6, 208, 1472, 1580, 1815
Csp6I GTAC 6 cut(s) 304, 1261, 1279, 1304, 1564, 1621
CviQI GTAC 6 cut(s) 304, 1261, 1279, 1304, 1564, 1621
DdeI CTNAG 5 cut(s) 178, 874, 1121, 1522, 2169
DpnI GATC 6 cut(s) 977, 1163, 1217, 1762, 2111, 2257
DpnII GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
DrdI GACNNNNNNGTC 2 cut(s) 932, 1727
DseDI GACNNNNNNGTC 2 cut(s) 932, 1727
EaeI YGGCCR 6 cut(s) 734, 1041, 1171, 1456, 1604, 2041
EagI CGGCCG 1 cut(s) 734
Eam1104I CTCTTC 2 cut(s) 197, 1620
EarI CTCTTC 2 cut(s) 197, 1620
Ecl136II GAGCTC 2 cut(s) 833, 1330
EclXI CGGCCG 1 cut(s) 734
Eco130I CCWWGG 2 cut(s) 657, 819
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 3 cut(s) 835, 1332, 1799
Eco31I GGTCTC 4 cut(s) 119, 940, 1309, 1430
Eco47I GGWCC 5 cut(s) 263, 857, 890, 1582, 1973
Eco52I CGGCCG 1 cut(s) 734
Eco53kI GAGCTC 2 cut(s) 833, 1330
Eco57I CTGAAG 1 cut(s) 2160
Eco88I CYCGRG 2 cut(s) 572, 899
EcoICRI GAGCTC 2 cut(s) 833, 1330
EcoO109I RGGNCCY 1 cut(s) 1795
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 909
EcoT14I CCWWGG 2 cut(s) 657, 819
EcoT38I GRGCYC 3 cut(s) 835, 1332, 1799
ErhI CCWWGG 2 cut(s) 657, 819
Esp3I CGTCTC 2 cut(s) 238, 693
FaqI GGGAC 3 cut(s) 7, 206, 1935
FauI CCCGC 2 cut(s) 1551, 1806
FauNDI CATATG 1 cut(s) 1243
FbaI TGATCA 1 cut(s) 975
FblI GTMKAC 1 cut(s) 905
Fnu4HI GCNGC 4 cut(s) 108, 390, 594, 1126
FokI GGATG 9 cut(s) 264, 328, 416, 791, 1109, 1403, 1630, 1918, 2252
FriOI GRGCYC 3 cut(s) 835, 1332, 1799
Fsp4HI GCNGC 4 cut(s) 108, 390, 594, 1126
FspBI CTAG 7 cut(s) 95, 599, 1019, 1307, 1646, 1754, 1784
GlaI GCGC 1 cut(s) 1705
GluI GCNGC 4 cut(s) 108, 390, 594, 1126
GsaI CCCAGC 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 1707
HgaI GACGC 5 cut(s) 6, 208, 1472, 1580, 1815
HhaI GCGC 1 cut(s) 1706
Hin1I GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
Hin6I GCGC 1 cut(s) 1704
HinP1I GCGC 1 cut(s) 1704
HincII GTYRAC 4 cut(s) 784, 906, 1789, 2095
HindII GTYRAC 4 cut(s) 784, 906, 1789, 2095
HinfI GANTC 2 cut(s) 622, 1520
HpaI GTTAAC 1 cut(s) 784
HphI GGTGA 6 cut(s) 955, 1174, 1225, 1580, 1918, 2234
Hpy166II GTNNAC 8 cut(s) 784, 890, 906, 1585, 1699, 1789, 2095, 2116
Hpy188III TCNNGA 8 cut(s) 335, 828, 836, 1159, 1333, 1732, 1754, 1994
Hpy8I GTNNAC 8 cut(s) 784, 890, 906, 1585, 1699, 1789, 2095, 2116
Hpy99I CGWCG 4 cut(s) 22, 1190, 1778, 1831
HpyAV CCTTC 5 cut(s) 184, 508, 663, 1687, 1896
HpyCH4III ACNGT 8 cut(s) 493, 957, 1237, 1568, 1729, 1839, 1936, 2092
HpyCH4IV ACGT 6 cut(s) 1302, 1365, 1431, 1530, 1845, 2049
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 728, 737, 830
HpyF3I CTNAG 5 cut(s) 178, 874, 1121, 1522, 2169
HpySE526I ACGT 6 cut(s) 1302, 1365, 1431, 1530, 1845, 2049
Hsp92I GRCGYC 5 cut(s) 17, 219, 1464, 1530, 1572
HspAI GCGC 1 cut(s) 1704
Kpn2I TCCGGA 1 cut(s) 827
KroI GCCGGC 1 cut(s) 736
KroNI GCCGGC 1 cut(s) 738
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 5 cut(s) 156, 830, 1327, 1806, 2206
Lsp1109I GCAGC 4 cut(s) 94, 401, 605, 1112
LweI GCATC 5 cut(s) 242, 394, 1087, 1356, 2243
MaeI CTAG 7 cut(s) 95, 599, 1019, 1307, 1646, 1754, 1784
MaeII ACGT 6 cut(s) 1302, 1365, 1431, 1530, 1845, 2049
MaeIII GTNAC 9 cut(s) 54, 605, 677, 961, 1030, 1151, 1568, 2045, 2222
MalI GATC 6 cut(s) 977, 1163, 1217, 1762, 2111, 2257
MbiI CCGCTC 2 cut(s) 1558, 1801
MboI GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
MfeI CAATTG 1 cut(s) 967
MflI RGATCY 1 cut(s) 2255
MhlI GDGCHC 6 cut(s) 471, 835, 1058, 1332, 1799, 2118
MlsI TGGCCA 1 cut(s) 1043
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 1 cut(s) 1514
MmeI TCCRAC 3 cut(s) 677, 1542, 1756
Mox20I TGGCCA 1 cut(s) 1043
MroI TCCGGA 1 cut(s) 827
MroNI GCCGGC 1 cut(s) 736
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 7 cut(s) 285, 525, 783, 1548, 1710, 2009, 2157
MslI CAYNNNNRTG 2 cut(s) 75, 2121
Msp20I TGGCCA 1 cut(s) 1043
MspA1I CMGCKG 2 cut(s) 269, 1373
MspCI CTTAAG 1 cut(s) 2008
MspR9I CCNGG 4 cut(s) 733, 911, 1793, 2204
MunI CAATTG 1 cut(s) 967
MvaI CCWGG 1 cut(s) 911
MvnI CGCG 1 cut(s) 940
MwoI GCNNNNNNNGC 4 cut(s) 45, 728, 737, 830
NaeI GCCGGC 1 cut(s) 738
NciI CCSGG 3 cut(s) 733, 1793, 2204
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 1243
NdeII GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
NgoMIV GCCGGC 1 cut(s) 736
NlaIV GGNNCC 3 cut(s) 664, 1796, 1797
NmuCI GTSAC 4 cut(s) 605, 961, 1568, 2222
NspI RCATGY 4 cut(s) 613, 1492, 2101, 2230
PaeI GCATGC 1 cut(s) 1492
PagI TCATGA 1 cut(s) 835
PaqCI CACCTGC 1 cut(s) 1075
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 2097
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 1572
PdiI GCCGGC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 622
PflFI GACNNNGTC 1 cut(s) 1504
PfoI TCCNGGA 1 cut(s) 2202
PkrI GCNGC 4 cut(s) 109, 391, 595, 1127
PleI GAGTC 1 cut(s) 1514
PpsI GAGTC 1 cut(s) 1514
Ppu21I YACGTR 1 cut(s) 2050
PscI ACATGT 1 cut(s) 2097
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 2 cut(s) 835, 1332
Psp1406I AACGTT 1 cut(s) 1845
Psp6I CCWGG 1 cut(s) 909
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 3 cut(s) 664, 1796, 1797
PspOMI GGGCCC 1 cut(s) 1795
PspPI GGNCC 9 cut(s) 263, 730, 857, 890, 1582, 1692, 1795, 1796, 1973
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 2255
PsyI GACNNNGTC 1 cut(s) 1504
PvuII CAGCTG 1 cut(s) 1373
RsaI GTAC 6 cut(s) 305, 1262, 1280, 1305, 1565, 1622
RsaNI GTAC 6 cut(s) 304, 1261, 1279, 1304, 1564, 1621
RseI CAYNNNNRTG 2 cut(s) 75, 2121
SacI GAGCTC 2 cut(s) 835, 1332
SalI GTCGAC 1 cut(s) 904
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 7 cut(s) 285, 525, 783, 1548, 1710, 2009, 2157
SatI GCNGC 4 cut(s) 108, 390, 594, 1126
Sau3AI GATC 6 cut(s) 975, 1161, 1215, 1760, 2109, 2255
Sau96I GGNCC 9 cut(s) 263, 730, 857, 890, 1582, 1692, 1795, 1796, 1973
SchI GAGTC 1 cut(s) 1514
ScrFI CCNGG 4 cut(s) 733, 911, 1793, 2204
SduI GDGCHC 6 cut(s) 471, 835, 1058, 1332, 1799, 2118
SfaNI GCATC 5 cut(s) 242, 394, 1087, 1356, 2243
SfcI CTRYAG 2 cut(s) 108, 1078
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 5 cut(s) 263, 857, 890, 1582, 1973
SmiMI CAYNNNNRTG 2 cut(s) 75, 2121
SmlI CTYRAG 2 cut(s) 1331, 2008
SmoI CTYRAG 2 cut(s) 1331, 2008
SpeI ACTAGT 1 cut(s) 1018
SphI GCATGC 1 cut(s) 1492
SseBI AGGCCT 1 cut(s) 437
SspMI CTAG 7 cut(s) 95, 599, 1019, 1307, 1646, 1754, 1784
SstI GAGCTC 2 cut(s) 835, 1332
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 4 cut(s) 731, 909, 1791, 2202
StyI CCWWGG 2 cut(s) 657, 819
TaaI ACNGT 8 cut(s) 493, 957, 1237, 1568, 1729, 1839, 1936, 2092
TaiI ACGT 6 cut(s) 1305, 1368, 1434, 1533, 1848, 2052
TaqI TCGA 4 cut(s) 905, 1005, 1271, 1554
TatI WGTACW 1 cut(s) 1260
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 7 cut(s) 285, 525, 783, 1548, 1710, 2009, 2157
Tru9I TTAA 7 cut(s) 285, 525, 783, 1548, 1710, 2009, 2157
TscAI CASTG 5 cut(s) 399, 451, 960, 1694, 1965
TseFI GTSAC 4 cut(s) 605, 961, 1568, 2222
TseI GCWGC 4 cut(s) 107, 389, 593, 1125
Tsp45I GTSAC 4 cut(s) 605, 961, 1568, 2222
TspDTI ATGAA 5 cut(s) 59, 919, 937, 1105, 1931
TspGWI ACGGA 4 cut(s) 542, 1203, 1762, 2075
TspRI CASTG 5 cut(s) 399, 451, 960, 1694, 1965
Tth111I GACNNNGTC 1 cut(s) 1504
Vha464I CTTAAG 1 cut(s) 2008
VneI GTGCAC 1 cut(s) 2114
VpaK11BI GGWCC 5 cut(s) 263, 857, 890, 1582, 1973
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 4 cut(s) 287, 1046, 1083, 1191
XbaI TCTAGA 1 cut(s) 1753
XceI RCATGY 4 cut(s) 613, 1492, 2101, 2230
XmiI GTMKAC 1 cut(s) 905
XspI CTAG 7 cut(s) 95, 599, 1019, 1307, 1646, 1754, 1784
ZraI GACGTC 1 cut(s) 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.