Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Forward (+)

8921882 .. 8924919

Gene Structure 3038 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g14510.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGTCGCACAATGTGTTCGGAACATTCACTACCGAGATTCCTGCAAGTGAATTATGTGTAAATTTTCCACCTCGCGTTAATTCGGTTGGCTTATGGAATAGATCTGAGCATACAGTCCTAAATATGACAGCTCAATTTGGGAAGCACTCTTTTCCACTTCCAAATCGTTCAATGCCATTGCTGGAATTAGAAATGACTTTCATGTACATCCTCACGCAGTTCTCGTATTTCTTTCTCAAGATGTTTGGCCTCTCTGGCTTTATCGCTCAAATTGTGGCTGGAATACTCCTAGGTGATGTAGGATTTCACAGAATTACCGAAAAAAAAGATATGCAAAATCCCATGTTTACCTTCACCAATCAAGAAATACTCAATCTAATAGCTTTGCTTGGATATACGCTCTTCTTATTCTTAATCGGAGTCCAAATCGATTTGAGCCTAATCTGCAAAACTGGAAGAAAGGCCGTGTATGTTGGTGTTTCAAATTTTCTTGTTCCTATGATGGTTGGCATGGGAGCTGTTCTTTACTTTCACCGTAACTGGCCATTAGGGAACGGACAAGCTGTTCAGCTTTTATTTGCTACAGCTATCCAGTGCTCGACTACGCTTCCAGTCATTGTTTCTCTCCTTAATCAGCTTAAGATTTTAAACTCGGAACTTGGCCGACTTGGATTATCTTCTGTACTCATCAGTGACCTACTTTATGTGTTCGTCGTCCTCATCGGATCCGCTGCTAAAAGCATGGACTATGATTTGTTAACAGGTGCGCTCAACTTAGGCATATTAGTGGCATTTGTTTTGATTGTTTTCTACGTCGGTCGCCCAATTATGTTTTGGATGATTAGACAAACCCCTAAGGGAAGAGTGGTGAAGAACAGCTATGTAATGTGCATCATGATAGCATTTTTGTTCTCCGGGGTGATTACACACTGGTGTGGTATGACTGTTACGGCCGGACCTTTTATCCTAGGTTTGGCAGTGCCTAGCGGACCGCCGTTGGGATCTACACTGGTAAAGAAGCTTGACTTTATCACCAACTCGGTGTTCATGCCGGTGTTTGTAATAACATGCATGATGAGGGTTACACTACTGCCAGAAGTGCCTCGGGCTCACCATTGGGTAGTTGTCGCAACTGGAGTTATCATCGTTACAGTATTTCTCAGCAAATTTCTGGCATCTTTGGTGCCTGCCTTGTGTTGCAAGATGCCTGTGAGTGATGCACTGGCACTTTCTCTCATATTGAGCAGTCAAGGCGTTCTTGAGATGGCTGTCTACTCTTTGGCTCGAGACACTTGGGTTATAACTTTATCAATGTATAATGCTCTACTTTGTGGCGTGGTTATTAATGCAATCTTCATTCCACCCTTGGTAAAACGATTGTACGATCCTAAAAGGAAGTACACAGGCTATGAGAAAAGAAACATCTCGAATTGCAAACCAAATTCCGAGCTTCGAATTCTAGCATGTGTCACTAGATGCGACAACACTCCGGCGATCATTAACCTCATCGATGCCGTGTGTCCGACACAAGAGAGCCCAATTTCTGTGTACGTCCTCCACCTCATCGAGCTAGTCGGCCGGACCACTCCGATCTTCATTTCTCACAACAAACAAAAAAGAACTGTCTCCAACTTCACCTCGTCGGAAAATGTGATCCTCTCATTTGATCACTTCCAAAGAGACAACGCCGGAGCAGTGAACGTGAACGTGTTCACATCTATTACTCCGGCGCAGTACATGCACGACGACATCTGCACCCTCGCCCTTGACAAGCTCACATCCCTTATAATCCTCCCCTTCCATCGAAAGTGGTCAATCCTTGGCTCAAATACTACAATTGAATCAGAGAACGAGAAAATCCGAGAAGTGAACATGAGCGTGCTTGAAACAGCGCCGTGTTCGGTTGGTATTCTTGTGAACCGCGGACGCATTCAAAGCATAGAGGCCGCAACCGCAACGATATCAGAGACATTTCGAGTGGCAATGATTTTCTTGGGAGGAAACGACGACAGGGAAGCACTCGTCTTCGCTAGACGCATGGCCAAGGACTCTTGCATAAGCCTCACGGTGATACATTTACTTGATTCAGTTGAATACAAGGAGGAAGACAACAACGGAGTCAAGCTGGACAAAGCCAAATGGGAGAGGCTACAAGAGAGGGAGATGTTGAGGAATATGGACATGAGTAGTGGGAACAGGTACGTGAGTTATGTGGAGGTGGTGGTGGAAGACGCAGCGCAAACGACGACAAAACTAAGGTCATTGTTGGATGAAGATGAATACGACATGTTCATTGTTGGGAGACGACACAAGGTGAAGAGTCCCCAGACTTCAGGACTGGAACAATGGTGTGAGTTCCCAGAGTTAGGGATTATCGGAGACCTGCTATCCTCCACCGACTCGCACAGTAAAGCTAGTGTATTGGTGATTCAGCAACAGCAACAAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

817

Amino Acids

90.93

Weight (kDa)

8.2

Isoelectric Point (pI)

40.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	74 - 458	3.4e-32	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	515 - 643	6.7e-12	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	659 - 814	6.8e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1301, 1787
Acc36I	ACCTGC	1 cut(s)	2391
AccB1I	GGYRCC	1 cut(s)	1183
AccI	GTMKAC	1 cut(s)	1272
AccII	CGCG	2 cut(s)	75, 1923
AciI	CCGC	7 cut(s)	729, 987, 992, 1921, 1923, 1947, 1953
AclWI	GGATC	5 cut(s)	720, 733, 1009, 1379, 1648
AcoI	YGGCCR	5 cut(s)	542, 661, 951, 1576, 2040
AcsI	RAATTY	5 cut(s)	61, 484, 1166, 1441, 1455
AcuI	CTGAAG	1 cut(s)	2316
AdeI	CACNNNGTG	2 cut(s)	13, 2314
AfaI	GTAC	7 cut(s)	206, 684, 1382, 1400, 1550, 1736, 2201
AfiI	CCNNNNNNNGG	3 cut(s)	973, 998, 2366
AflII	CTTAAG	1 cut(s)	638
AflIII	ACRYGT	2 cut(s)	1707, 2286
AgsI	TTSAA	6 cut(s)	171, 483, 1841, 1886, 1934, 2093
AjuI	GAANNNNNNNTTGG	2 cut(s)	1028, 1060
AleI	CACNNNNGTG	2 cut(s)	931, 933
AloI	GAACNNNNNNTCC	2 cut(s)	549, 581
Alw21I	GWGCWC	1 cut(s)	599
Alw26I	GTCTC	6 cut(s)	1281, 1630, 1674, 1961, 2296, 2373
AlwI	GGATC	5 cut(s)	720, 733, 1009, 1379, 1648
Ama87I	CYCGRG	2 cut(s)	1104, 1284
AoxI	GGCC	8 cut(s)	247, 462, 542, 661, 951, 1576, 1944, 2040
ApeKI	GCWGC	2 cut(s)	731, 2234
ApoI	RAATTY	5 cut(s)	61, 484, 1166, 1441, 1455
ArsI	GACNNNNNNTTYG	2 cut(s)	2243, 2275
AseI	ATTAAT	1 cut(s)	1344
Asp700I	GAANNNNTTC	1 cut(s)	1709
AspA2I	CCTAGG	2 cut(s)	289, 967
AspLEI	GCGC	4 cut(s)	769, 1732, 1894, 2239
AspS9I	GGNCC	3 cut(s)	956, 989, 1581
AsuC2I	CCSGG	1 cut(s)	916
AsuII	TTCGAA	1 cut(s)	1453
AvaI	CYCGRG	2 cut(s)	1104, 1284
AvaII	GGWCC	3 cut(s)	956, 989, 1581
AvrII	CCTAGG	2 cut(s)	289, 967
AxyI	CCTNAGG	1 cut(s)	855
BaeI	ACNNNNGTAYC	2 cut(s)	2191, 2224
BalI	TGGCCA	2 cut(s)	544, 2042
BamHI	GGATCC	1 cut(s)	725
BanI	GGYRCC	1 cut(s)	1183
BanII	GRGCYC	2 cut(s)	1111, 1538
BbsI	GAAGAC	3 cut(s)	2017, 2112, 2235
Bbv12I	GWGCWC	1 cut(s)	599
BbvI	GCAGC	2 cut(s)	718, 2246
BccI	CCATC	3 cut(s)	496, 1258, 1809
BceAI	ACGGC	5 cut(s)	449, 966, 979, 1499, 1879
BcgI	CGANNNNNNTGC	2 cut(s)	23, 57
BclI	TGATCA	1 cut(s)	1666
BcnI	CCSGG	1 cut(s)	916
BcoDI	GTCTC	6 cut(s)	1281, 1630, 1674, 1961, 2296, 2373
BfaI	CTAG	8 cut(s)	290, 968, 984, 1460, 1473, 1571, 2031, 2415
BfmI	CTRYAG	1 cut(s)	582
BfoI	RGCGCY	1 cut(s)	1895
BfrI	CTTAAG	1 cut(s)	638
BfuAI	ACCTGC	1 cut(s)	2391
BglI	GCCNNNNNGGC	1 cut(s)	255
BglII	AGATCT	1 cut(s)	101
BisI	GCNGC	3 cut(s)	732, 1947, 2235
BlnI	CCTAGG	2 cut(s)	289, 967
BlsI	GCNGC	3 cut(s)	733, 1948, 2236
Bme1390I	CCNGG	1 cut(s)	916
Bme18I	GGWCC	3 cut(s)	956, 989, 1581
BmeT110I	CYCGRG	2 cut(s)	1104, 1284
BmgT120I	GGNCC	3 cut(s)	956, 989, 1581
BmiI	GGNNCC	2 cut(s)	727, 1185
BmrFI	CCNGG	1 cut(s)	916
BmsI	GCATC	6 cut(s)	900, 1184, 1194, 1207, 1466, 1501
BpiI	GAAGAC	3 cut(s)	2017, 2112, 2235
BpmI	CTGGAG	1 cut(s)	1155
Bpu14I	TTCGAA	1 cut(s)	1453
BpuEI	CTTGAG	2 cut(s)	221, 1280
BpuMI	CCSGG	1 cut(s)	916
Bsa29I	ATCGAT	2 cut(s)	429, 1509
BsaAI	YACGTR	1 cut(s)	2203
BsaI	GGTCTC	1 cut(s)	2373
BsaJI	CCNNGG	8 cut(s)	289, 915, 967, 1103, 1365, 1819, 1921, 2043
Bsc4I	CCNNNNNNNGG	3 cut(s)	973, 998, 2366
Bse118I	RCCGGY	1 cut(s)	1051
Bse1I	ACTGG	9 cut(s)	457, 545, 592, 611, 935, 1014, 1138, 1227, 2343
Bse21I	CCTNAGG	1 cut(s)	855
Bse3DI	GCAATG	2 cut(s)	176, 1989
BseCI	ATCGAT	2 cut(s)	429, 1509
BseDI	CCNNGG	8 cut(s)	289, 915, 967, 1103, 1365, 1819, 1921, 2043
BseGI	GGATG	4 cut(s)	207, 843, 1778, 2275
BseLI	CCNNNNNNNGG	3 cut(s)	973, 998, 2366
BseMI	GCAATG	2 cut(s)	176, 1989
BseMII	CTCAG	2 cut(s)	96, 1174
BseNI	ACTGG	9 cut(s)	457, 545, 592, 611, 935, 1014, 1138, 1227, 2343
BseX3I	CGGCCG	2 cut(s)	951, 1576
BseXI	GCAGC	2 cut(s)	718, 2246
BsgI	GTGCAG	1 cut(s)	1738
Bsh1236I	CGCG	2 cut(s)	75, 1923
Bsh1285I	CGRYCG	3 cut(s)	820, 954, 1579
BshFI	GGCC	8 cut(s)	249, 464, 544, 663, 953, 1578, 1946, 2042
BshNI	GGYRCC	1 cut(s)	1183
BshVI	ATCGAT	2 cut(s)	429, 1509
BsiEI	CGRYCG	3 cut(s)	820, 954, 1579
BsiHKAI	GWGCWC	1 cut(s)	599
BsiHKCI	CYCGRG	2 cut(s)	1104, 1284
BsiSI	CCGG	7 cut(s)	915, 954, 1052, 1490, 1579, 1689, 1727
BslFI	GGGAC	1 cut(s)	2307
BslI	CCNNNNNNNGG	3 cut(s)	973, 998, 2366
BsmAI	GTCTC	6 cut(s)	1281, 1630, 1674, 1961, 2296, 2373
BsmBI	CGTCTC	1 cut(s)	2296
BsmFI	GGGAC	1 cut(s)	2307
BsmI	GAATGC	1 cut(s)	1929
BsnI	GGCC	8 cut(s)	249, 464, 544, 663, 953, 1578, 1946, 2042
Bso31I	GGTCTC	1 cut(s)	2373
BsoBI	CYCGRG	2 cut(s)	1104, 1284
Bsp119I	TTCGAA	1 cut(s)	1453
Bsp1286I	GDGCHC	3 cut(s)	599, 1111, 1538
Bsp1407I	TGTACA	1 cut(s)	204
Bsp143I	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
BspACI	CCGC	7 cut(s)	729, 987, 992, 1921, 1923, 1947, 1953
BspANI	GGCC	8 cut(s)	249, 464, 544, 663, 953, 1578, 1946, 2042
BspCNI	CTCAG	2 cut(s)	97, 1173
BspDI	ATCGAT	2 cut(s)	429, 1509
BspFNI	CGCG	2 cut(s)	75, 1923
BspHI	TCATGA	1 cut(s)	894
BspLI	GGNNCC	2 cut(s)	727, 1185
BspMI	ACCTGC	1 cut(s)	2391
BspPI	GGATC	5 cut(s)	720, 733, 1009, 1379, 1648
BspQI	GCTCTTC	1 cut(s)	407
BspT104I	TTCGAA	1 cut(s)	1453
BspT107I	GGYRCC	1 cut(s)	1183
BspTI	CTTAAG	1 cut(s)	638
BspTNI	GGTCTC	1 cut(s)	2373
BsrDI	GCAATG	2 cut(s)	176, 1989
BsrFI	RCCGGY	1 cut(s)	1051
BsrGI	TGTACA	1 cut(s)	204
BsrI	ACTGG	9 cut(s)	457, 545, 592, 611, 935, 1014, 1138, 1227, 2343
BssAI	RCCGGY	1 cut(s)	1051
BssECI	CCNNGG	8 cut(s)	289, 915, 967, 1103, 1365, 1819, 1921, 2043
BssMI	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
BssT1I	CCWWGG	5 cut(s)	289, 967, 1365, 1819, 2043
Bst4CI	ACNGT	7 cut(s)	115, 536, 946, 1153, 1624, 2068, 2408
Bst6I	CTCTTC	3 cut(s)	407, 856, 2312
BstAFI	CTTAAG	1 cut(s)	638
BstAPI	GCANNNNNTGC	1 cut(s)	1738
BstAUI	TGTACA	1 cut(s)	204
BstBAI	YACGTR	1 cut(s)	2203
BstBI	TTCGAA	1 cut(s)	1453
BstC8I	GCNNGC	2 cut(s)	1188, 1880
BstDEI	CTNAG	5 cut(s)	105, 775, 855, 1160, 2255
BstDSI	CCRYGG	1 cut(s)	1921
BstF5I	GGATG	4 cut(s)	207, 843, 1778, 2275
BstFNI	CGCG	2 cut(s)	75, 1923
BstH2I	RGCGCY	1 cut(s)	1895
BstHHI	GCGC	4 cut(s)	769, 1732, 1894, 2239
BstKTI	GATC	8 cut(s)	104, 728, 1004, 1387, 1497, 1593, 1656, 1669
BstMAI	GTCTC	6 cut(s)	1281, 1630, 1674, 1961, 2296, 2373
BstMBI	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
BstMCI	CGRYCG	3 cut(s)	820, 954, 1579
BstMWI	GCNNNNNNNGC	7 cut(s)	255, 444, 1099, 1251, 1738, 1935, 1952
BstNSI	RCATGY	4 cut(s)	1071, 1467, 1741, 2290
BstSCI	CCNGG	1 cut(s)	914
BstSFI	CTRYAG	1 cut(s)	582
BstUI	CGCG	2 cut(s)	75, 1923
BstV1I	GCAGC	2 cut(s)	718, 2246
BstV2I	GAAGAC	3 cut(s)	2017, 2112, 2235
BstX2I	RGATCY	3 cut(s)	101, 725, 1001
BstYI	RGATCY	3 cut(s)	101, 725, 1001
BstZI	CGGCCG	2 cut(s)	951, 1576
Bsu15I	ATCGAT	2 cut(s)	429, 1509
Bsu36I	CCTNAGG	1 cut(s)	855
BsuRI	GGCC	8 cut(s)	249, 464, 544, 663, 953, 1578, 1946, 2042
BsuTUI	ATCGAT	2 cut(s)	429, 1509
BtgI	CCRYGG	1 cut(s)	1921
BtsCI	GGATG	4 cut(s)	207, 843, 1778, 2275
BtsI	GCAGTG	2 cut(s)	984, 1701
BtsIMutI	CAGTG	7 cut(s)	599, 697, 928, 984, 1007, 1220, 1701
BveI	ACCTGC	1 cut(s)	2391
Cac8I	GCNNGC	2 cut(s)	1188, 1880
CciI	TCATGA	1 cut(s)	894
CfoI	GCGC	4 cut(s)	769, 1732, 1894, 2239
Cfr10I	RCCGGY	1 cut(s)	1051
Cfr13I	GGNCC	3 cut(s)	956, 989, 1581
Cfr42I	CCGCGG	1 cut(s)	1924
ClaI	ATCGAT	2 cut(s)	429, 1509
CpoI	CGGWCCG	1 cut(s)	989
CseI	GACGC	3 cut(s)	1935, 2043, 2240
Csp6I	GTAC	7 cut(s)	205, 683, 1381, 1399, 1549, 1735, 2200
CspI	CGGWCCG	1 cut(s)	989
CviQI	GTAC	7 cut(s)	205, 683, 1381, 1399, 1549, 1735, 2200
DdeI	CTNAG	5 cut(s)	105, 775, 855, 1160, 2255
DpnI	GATC	8 cut(s)	103, 727, 1003, 1386, 1496, 1592, 1655, 1668
DpnII	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
DraI	TTTAAA	1 cut(s)	648
DraIII	CACNNNGTG	2 cut(s)	13, 2314
EaeI	YGGCCR	5 cut(s)	542, 661, 951, 1576, 2040
EagI	CGGCCG	2 cut(s)	951, 1576
Eam1104I	CTCTTC	3 cut(s)	407, 856, 2312
EarI	CTCTTC	3 cut(s)	407, 856, 2312
EclXI	CGGCCG	2 cut(s)	951, 1576
Eco130I	CCWWGG	5 cut(s)	289, 967, 1365, 1819, 2043
Eco24I	GRGCYC	2 cut(s)	1111, 1538
Eco31I	GGTCTC	1 cut(s)	2373
Eco32I	GATATC	1 cut(s)	1962
Eco47I	GGWCC	3 cut(s)	956, 989, 1581
Eco52I	CGGCCG	2 cut(s)	951, 1576
Eco57I	CTGAAG	1 cut(s)	2316
Eco81I	CCTNAGG	1 cut(s)	855
Eco88I	CYCGRG	2 cut(s)	1104, 1284
EcoRI	GAATTC	1 cut(s)	1455
EcoRV	GATATC	1 cut(s)	1962
EcoT14I	CCWWGG	5 cut(s)	289, 967, 1365, 1819, 2043
EcoT22I	ATGCAT	1 cut(s)	1073
EcoT38I	GRGCYC	2 cut(s)	1111, 1538
ErhI	CCWWGG	5 cut(s)	289, 967, 1365, 1819, 2043
Esp3I	CGTCTC	1 cut(s)	2296
FalI	AAGNNNNNCTT	4 cut(s)	1010, 1042, 1242, 1274
FaqI	GGGAC	1 cut(s)	2307
FbaI	TGATCA	1 cut(s)	1666
FblI	GTMKAC	1 cut(s)	1272
Fnu4HI	GCNGC	3 cut(s)	732, 1947, 2235
FokI	GGATG	4 cut(s)	194, 850, 1765, 2282
FriOI	GRGCYC	2 cut(s)	1111, 1538
Fsp4HI	GCNGC	3 cut(s)	732, 1947, 2235
FspBI	CTAG	8 cut(s)	290, 968, 984, 1460, 1473, 1571, 2031, 2415
GlaI	GCGC	4 cut(s)	768, 1731, 1893, 2238
GluI	GCNGC	3 cut(s)	732, 1947, 2235
GsuI	CTGGAG	1 cut(s)	1155
HaeII	RGCGCY	1 cut(s)	1895
HaeIII	GGCC	8 cut(s)	249, 464, 544, 663, 953, 1578, 1946, 2042
HapII	CCGG	7 cut(s)	915, 954, 1052, 1490, 1579, 1689, 1727
HgaI	GACGC	3 cut(s)	1935, 2043, 2240
HhaI	GCGC	4 cut(s)	769, 1732, 1894, 2239
Hin6I	GCGC	4 cut(s)	767, 1730, 1892, 2237
HinP1I	GCGC	4 cut(s)	767, 1730, 1892, 2237
HincII	GTYRAC	1 cut(s)	759
HindII	GTYRAC	1 cut(s)	759
HindIII	AAGCTT	1 cut(s)	1019
HinfI	GANTC	9 cut(s)	37, 420, 1841, 2048, 2084, 2118, 2320, 2399, 2428
HpaI	GTTAAC	1 cut(s)	759
HpaII	CCGG	7 cut(s)	915, 954, 1052, 1490, 1579, 1689, 1727
Hpy188III	TCNNGA	7 cut(s)	238, 362, 895, 1259, 1286, 1426, 2334
Hpy99I	CGWCG	6 cut(s)	716, 818, 1645, 1748, 2009, 2248
HpyAV	CCTTC	2 cut(s)	361, 1807
HpyCH4III	ACNGT	7 cut(s)	115, 536, 946, 1153, 1624, 2068, 2408
HpyCH4IV	ACGT	5 cut(s)	813, 1551, 1701, 1707, 2202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	255, 444, 1099, 1251, 1738, 1935, 1952
HpyF3I	CTNAG	5 cut(s)	105, 775, 855, 1160, 2255
HpySE526I	ACGT	5 cut(s)	813, 1551, 1701, 1707, 2202
HspAI	GCGC	4 cut(s)	767, 1730, 1892, 2237
Ksp22I	TGATCA	1 cut(s)	1666
KspAI	GTTAAC	1 cut(s)	759
KspI	CCGCGG	1 cut(s)	1924
Kzo9I	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
LguI	GCTCTTC	1 cut(s)	407
LmnI	GCTCC	2 cut(s)	515, 1691
Lsp1109I	GCAGC	2 cut(s)	718, 2246
LweI	GCATC	6 cut(s)	900, 1184, 1194, 1207, 1466, 1501
MaeI	CTAG	8 cut(s)	290, 968, 984, 1460, 1473, 1571, 2031, 2415
MaeII	ACGT	5 cut(s)	813, 1551, 1701, 1707, 2202
MaeIII	GTNAC	6 cut(s)	536, 692, 946, 1081, 1147, 1468
MalI	GATC	8 cut(s)	103, 727, 1003, 1386, 1496, 1592, 1655, 1668
MboI	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
MfeI	CAATTG	1 cut(s)	1836
MflI	RGATCY	3 cut(s)	101, 725, 1001
MhlI	GDGCHC	3 cut(s)	599, 1111, 1538
MlsI	TGGCCA	2 cut(s)	544, 2042
MluNI	TGGCCA	2 cut(s)	544, 2042
MlyI	GAGTC	5 cut(s)	429, 2042, 2127, 2329, 2393
MmeI	TCCRAC	4 cut(s)	1547, 1623, 1653, 2247
Mox20I	TGGCCA	2 cut(s)	544, 2042
Mph1103I	ATGCAT	1 cut(s)	1073
MroXI	GAANNNNTTC	1 cut(s)	1709
MscI	TGGCCA	2 cut(s)	544, 2042
MseI	TTAA	8 cut(s)	78, 413, 630, 639, 647, 758, 1344, 1500
MslI	CAYNNNNRTG	5 cut(s)	785, 931, 933, 1052, 1877
Msp20I	TGGCCA	2 cut(s)	544, 2042
MspA1I	CMGCKG	2 cut(s)	731, 1923
MspCI	CTTAAG	1 cut(s)	638
MspI	CCGG	7 cut(s)	915, 954, 1052, 1490, 1579, 1689, 1727
MspR9I	CCNGG	1 cut(s)	916
MunI	CAATTG	1 cut(s)	1836
Mva1269I	GAATGC	1 cut(s)	1929
MvnI	CGCG	2 cut(s)	75, 1923
MwoI	GCNNNNNNNGC	7 cut(s)	255, 444, 1099, 1251, 1738, 1935, 1952
NciI	CCSGG	1 cut(s)	916
NdeII	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
NlaIV	GGNNCC	2 cut(s)	727, 1185
NmuCI	GTSAC	2 cut(s)	692, 1468
NsiI	ATGCAT	1 cut(s)	1073
NspI	RCATGY	4 cut(s)	1071, 1467, 1741, 2290
NspV	TTCGAA	1 cut(s)	1453
OliI	CACNNNNGTG	2 cut(s)	931, 933
PaeR7I	CTCGAG	1 cut(s)	1284
PagI	TCATGA	1 cut(s)	894
PciI	ACATGT	1 cut(s)	2286
PciSI	GCTCTTC	1 cut(s)	407
PcsI	WCGNNNNNNNCGW	1 cut(s)	221
PctI	GAATGC	1 cut(s)	1929
PdmI	GAANNNNTTC	1 cut(s)	1709
PfeI	GAWTC	4 cut(s)	37, 1841, 2084, 2428
PkrI	GCNGC	3 cut(s)	733, 1948, 2236
PleI	GAGTC	5 cut(s)	428, 2042, 2126, 2328, 2393
PpsI	GAGTC	5 cut(s)	428, 2042, 2126, 2328, 2393
Ppu21I	YACGTR	1 cut(s)	2203
PscI	ACATGT	1 cut(s)	2286
PshBI	ATTAAT	1 cut(s)	1344
PsiI	TTATAA	2 cut(s)	1301, 1787
PspN4I	GGNNCC	2 cut(s)	727, 1185
PspPI	GGNCC	3 cut(s)	956, 989, 1581
PsrI	GAACNNNNNNTAC	2 cut(s)	13, 45
PsuI	RGATCY	3 cut(s)	101, 725, 1001
RsaI	GTAC	7 cut(s)	206, 684, 1382, 1400, 1550, 1736, 2201
RsaNI	GTAC	7 cut(s)	205, 683, 1381, 1399, 1549, 1735, 2200
RseI	CAYNNNNRTG	5 cut(s)	785, 931, 933, 1052, 1877
Rsr2I	CGGWCCG	1 cut(s)	989
RsrII	CGGWCCG	1 cut(s)	989
SacII	CCGCGG	1 cut(s)	1924
SapI	GCTCTTC	1 cut(s)	407
SaqAI	TTAA	8 cut(s)	78, 413, 630, 639, 647, 758, 1344, 1500
SatI	GCNGC	3 cut(s)	732, 1947, 2235
Sau3AI	GATC	8 cut(s)	101, 725, 1001, 1384, 1494, 1590, 1653, 1666
Sau96I	GGNCC	3 cut(s)	956, 989, 1581
SchI	GAGTC	5 cut(s)	429, 2042, 2127, 2329, 2393
ScrFI	CCNGG	1 cut(s)	916
SduI	GDGCHC	3 cut(s)	599, 1111, 1538
SfaNI	GCATC	6 cut(s)	900, 1184, 1194, 1207, 1466, 1501
SfcI	CTRYAG	1 cut(s)	582
Sfr274I	CTCGAG	1 cut(s)	1284
Sfr303I	CCGCGG	1 cut(s)	1924
SfuI	TTCGAA	1 cut(s)	1453
SgrBI	CCGCGG	1 cut(s)	1924
SinI	GGWCC	3 cut(s)	956, 989, 1581
SlaI	CTCGAG	1 cut(s)	1284
SmiMI	CAYNNNNRTG	5 cut(s)	785, 931, 933, 1052, 1877
SmlI	CTYRAG	4 cut(s)	236, 638, 1259, 1284
SmoI	CTYRAG	4 cut(s)	236, 638, 1259, 1284
SsiI	CCGC	7 cut(s)	729, 987, 992, 1921, 1923, 1947, 1953
SspMI	CTAG	8 cut(s)	290, 968, 984, 1460, 1473, 1571, 2031, 2415
StyD4I	CCNGG	1 cut(s)	914
StyI	CCWWGG	5 cut(s)	289, 967, 1365, 1819, 2043
TaaI	ACNGT	7 cut(s)	115, 536, 946, 1153, 1624, 2068, 2408
TaiI	ACGT	5 cut(s)	816, 1554, 1704, 1710, 2205
TaqI	TCGA	9 cut(s)	429, 599, 1285, 1427, 1453, 1509, 1566, 1804, 1975
TaqII	GACCGA	1 cut(s)	806
TatI	WGTACW	4 cut(s)	204, 682, 1398, 1734
TauI	GCSGC	1 cut(s)	1949
TfiI	GAWTC	4 cut(s)	37, 1841, 2084, 2428
Tru1I	TTAA	8 cut(s)	78, 413, 630, 639, 647, 758, 1344, 1500
Tru9I	TTAA	8 cut(s)	78, 413, 630, 639, 647, 758, 1344, 1500
TscAI	CASTG	7 cut(s)	599, 697, 935, 984, 1014, 1227, 1701
TseFI	GTSAC	2 cut(s)	692, 1468
TseI	GCWGC	2 cut(s)	731, 2234
Tsp45I	GTSAC	2 cut(s)	692, 1468
TspDTI	ATGAA	7 cut(s)	190, 1036, 1345, 1585, 2281, 2286, 2292
TspGWI	ACGGA	2 cut(s)	570, 2130
TspRI	CASTG	7 cut(s)	599, 697, 935, 984, 1014, 1227, 1701
Vha464I	CTTAAG	1 cut(s)	638
VpaK11BI	GGWCC	3 cut(s)	956, 989, 1581
VspI	ATTAAT	1 cut(s)	1344
XapI	RAATTY	5 cut(s)	61, 484, 1166, 1441, 1455
XceI	RCATGY	4 cut(s)	1071, 1467, 1741, 2290
XcmI	CCANNNNNNNNNTGG	1 cut(s)	831
XhoI	CTCGAG	1 cut(s)	1284
XmaJI	CCTAGG	2 cut(s)	289, 967
XmiI	GTMKAC	1 cut(s)	1272
XmnI	GAANNNNTTC	1 cut(s)	1709
XspI	CTAG	8 cut(s)	290, 968, 984, 1460, 1473, 1571, 2031, 2415
Zsp2I	ATGCAT	1 cut(s)	1073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.