Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001192

Physical Location & Seq

Genomic Coordinates Reverse (-)

4569996 .. 4573635

Gene Structure 3640 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1057300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGCGACTCACACGGCGGCCAACCTAACCAGTTCGGAAACGAGAGTTACTGAGTGTACCGAGTTCCCTCCTAACGTGCATTCACCTGGCCTGTACTCGAGAAACTACACAGATAGTGATAATCAGCTGTATACCCTTCCAAATCTGGAGATACAGATGGTTTTAATTTTTATCATCACACAAGCCTTCTATTTCGTTTTTCGCTATTTTGGAGTTCCAAGGTTCACCTGCCAAATTATAACCGGCATAATCCTCAGCCCTACACTCCTTGGGCACTACGAATTCTACAAGAACTTCTTGTTTAATGTAAACAGCCAAGAATCACTCGGTCTTCTGTCTGAGTTGGGTTATGGGCTCTTCATGTTTCTAATTGGAGTGAAAATGGATTTGGGAATGATAAAAAGGACAGGTAAAAAAGGCTTTTACACTGGTGTTGCATGCGTCGTGGCGCCTTTAGTAGTCACTATGGCTGTCCAAGCCACGCTCAACGCTAAAATGTTCAGCCTAACCAAACACGAACAGTTTGAACTTCCATTCATGACTCTGTTACATTCAATGACTCCATTTCCGGTTGTGGCTTGCCTTCTTGAGGAGCTGAAAATTCTGAATTCGGAGATTGGTCGATTAGGCATGTCTGCCGCATTAGTCAGTGACACAATCAGTGTGTTCCTTCAATTAGTGGGGCAAATTCCTAGAATGGGAAATGAGACTCATGATACAAGGGGTATGATAAAGGCACTAGCAGCAGCCGTTGGCTATATTATTGTTGTGATTATCATAGTTCGACCGGTAATGTTGCGGATTATCAGGCAGACCCCGGAAAATAGGCCTGTCAAGAAAGGCTATGTCAATATGACCATCATCCTTGTGCTCCTATCCGGCCTGCTTTCGCATCTTTACGGTCAGGGCTTTTATTTCGGCCCATTTATATTTGGTTTGGCTGTCCCAGCCGGACCACCATTAGGATCAGCTATTGAAGAAAAGCTCAACATATTCGTTTCTGATGTGATGCTTCCCATCTTTGTGACTACAAGTGCAATGAGGACAAATTTATGGTCCCTGGAACATATCTTAACTGACTCTTTTGCACAAATTAATGGAATCCTCATCCTCGTCGCTCTTGTGACCAAATTTATAGCCAGTCTGATTGTTCCCTTGTATTGCAACATGCCCTTCGTCGATGCCTTATCAATTGCTTTCATTTTGTGCTGCAAAGGCATTGTCAATCTTGCCTCCTATACCGATTTAAGAGACAGTCAGACCATCAGTGAATCAGCATTCGCTTTGGCAATGACTAGCGTCATCGTGACTGCAACTCTTATGCCAATCCTGGTGAATTGCCTGTATGATCCTTCTAGAAAATATGCCGGCTACCAAAGAAGGAATATGATGCATTTGAAACCGAATTCTGAGCTCAGAATACTTGCTTGCATTCATAGGTCAGCTAACATGCCTGCACTCATCAATCTACTTGATGCAGCATGTCCATCTAGAGACAACCCGATTGCTGTTCATGTCCTTCACCTTATTGAATTAGTGGGCAGAGCCTCTCCTCTTTTCATCTCCCACCAAATGCAGAAAAAATCTACTTCCAGTACTTCATACTCAGACAATGTCATTCTTTACTTCAATCACTTTGTTAGAGAAAATCTTGGTGCTGTATCTTTAAGCATCTTCACAGCAATTTCTCCACCAAAATACATGCATGAAGACATATGCACCCTAGCATTGGACAAACTCTGTTCCCTAATTGTGCTCCCATTTCACCGAAGATGGTCGATGGACGGGTCAGTGGAATCAGAAGACAATAGCGTAAGGAACCTAAATTGCAGTGTCCTAGAAAGAGCCCCATGTTCGGTTGGAATCCTGGTAGACCGCGGCCATTTAGGACGGTCCACCTCCGCTGTAGCACCCGAGTCATCCTTCTCCGTGGCTATAATCTTCTTGGGAGGGAAAGACGATAGGGAAGCATTGTCATTTGCCAAGAGAATGGCAAACGACTCCACAATAAGCCTGACTGTGATCCGACTTGTTGCCGCAAACTCTGAAGCCTGTACGGATTGGGATAGTGTACTTGATGCCGAGGAAATGAAGGGTTTTAGATATAACGATGTGGGTGAGGGTTTTGTGATATACTTGGAGGAGGTTGTGATAGATGGCCCTCAGACGGCTTTAGTGCTTCGGTCTATGGTAGATGAGTATGACCTAATTATAGTTGGGAGAAGGCATATGGTGCAGTCCCAGCAGACTGCGGGGCTTTCGGAATGGAGTGAATTTCCGGAGCTAGGGACCGTAGGGGACTTGCTTGCATCACCGGACATCAATTGTAGAGCTTCAATTCTGGTGGTACAGCAGCAAAGGATGATGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

791

Amino Acids

87.4

Weight (kDa)

6.16

Isoelectric Point (pI)

49.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	62 - 444	3e-33	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	504 - 631	7.9e-14	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	641 - 786	3.6e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44900 AT5G22900
fragaria_vesca	FvH4_3g06341 FvH4_3g23050 FvH4_3g39831 FvH4_6g14510 FvH4_7g14610
malus_domestica	MD02G1295300.v1.1 MD03G1057300.v1.1 MD05G1291400.v1.1 MD10G1267300.v1.1 MD11G1059200.v1.1 MD14G1082300.v1.1
prunus_persica	Prupe.1G346300_v2.0.a1 Prupe.2G029000_v2.0.a1 Prupe.4G074200_v2.0.a1 Prupe.6G047400_v2.0.a1 Prupe.6G047500_v2.0.a1 Prupe.6G047600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0356111 RchiOBHm_Chr1g0356121 RchiOBHm_Chr4g0446581 RchiOBHm_Chr5g0071781
rosa_laevigata	RLG00000005649 RLG00000028106 RLG00000036246 RLG00000036247 RLG00000036248
rosa_multiflora	Rmu_sc0001113.1_g000009 Rmu_sc0002569.1_g000023 Rmu_sc0003229.1_g000013 Rmu_sc0036166.1_g000001 Rmu_ssc0000144.1_g000006
rosa_roxburghii	Rroxscaffold_1G00009380 Rroxscaffold_1G00009410 Rroxscaffold_4G00299800 Rroxscaffold_5G00386970
rosa_rugosa	Rorug04G0372400 Rorug05G0414600 Rorug05G0414700
rosa_samantha	Rh1AG262500 Rh1BG231000 Rh1CG245800 Rh4AG434100 Rh4BG443300 Rh4CG460600 Rh4DG442100 Rh5AG470700 Rh5BG490400 Rh5CG514400 Rh5DG501400 Rh5DG501600 Rh5DG501700
rosa_wichuraiana	Rw1G023190 Rw4G037110 Rw5G043740 Rw5G043750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	239
AarI	CACCTGC	1 cut(s)	236
Acc36I	ACCTGC	1 cut(s)	236
AccB1I	GGYRCC	1 cut(s)	448
AccI	GTMKAC	2 cut(s)	131, 1872
AccII	CGCG	1 cut(s)	1878
AccIII	TCCGGA	1 cut(s)	2278
AciI	CCGC	8 cut(s)	17, 639, 799, 1876, 1878, 1902, 2037, 2252
AclWI	GGATC	3 cut(s)	973, 1343, 2017
AcoI	YGGCCR	2 cut(s)	18, 1879
AcsI	RAATTY	8 cut(s)	281, 600, 607, 687, 1048, 1130, 1405, 2273
AcuI	CTGAAG	1 cut(s)	2067
AcyI	GRCGYC	1 cut(s)	449
AfaI	GTAC	6 cut(s)	58, 95, 1597, 2056, 2073, 2349
AfiI	CCNNNNNNNGG	7 cut(s)	589, 698, 962, 1729, 1855, 2167, 2285
AgeI	ACCGGT	1 cut(s)	787
AgsI	TTSAA	8 cut(s)	527, 555, 674, 977, 1399, 1532, 1630, 2337
AjnI	CCWGG	4 cut(s)	85, 1059, 1329, 1866
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
AluBI	AGCT	8 cut(s)	127, 595, 971, 985, 1414, 1445, 2284, 2333
AluI	AGCT	8 cut(s)	127, 595, 971, 985, 1414, 1445, 2284, 2333
Alw21I	GWGCWC	3 cut(s)	873, 1416, 1758
Alw26I	GTCTC	3 cut(s)	701, 1245, 1488
AlwI	GGATC	3 cut(s)	973, 1343, 2017
Ama87I	CYCGRG	2 cut(s)	97, 1913
Aor13HI	TCCGGA	1 cut(s)	2278
AoxI	GGCC	7 cut(s)	18, 88, 827, 880, 919, 1879, 2158
ApeKI	GCWGC	5 cut(s)	743, 746, 1209, 1478, 2353
ApoI	RAATTY	8 cut(s)	281, 600, 607, 687, 1048, 1130, 1405, 2273
ArsI	GACNNNNNNTTYG	4 cut(s)	1040, 1072, 1206, 1238
AseI	ATTAAT	1 cut(s)	1095
AsiGI	ACCGGT	1 cut(s)	787
AspLEI	GCGC	1 cut(s)	451
AspS9I	GGNCC	6 cut(s)	920, 953, 1056, 1893, 2159, 2289
AsuC2I	CCSGG	1 cut(s)	818
AsuHPI	GGTGA	7 cut(s)	75, 217, 1345, 1514, 1757, 2129, 2304
AvaI	CYCGRG	2 cut(s)	97, 1913
AvaII	GGWCC	4 cut(s)	953, 1056, 1893, 2289
BaeGI	GKGCMC	1 cut(s)	276
BanI	GGYRCC	1 cut(s)	448
BanII	GRGCYC	3 cut(s)	357, 1416, 1849
BbsI	GAAGAC	3 cut(s)	323, 1716, 1809
Bbv12I	GWGCWC	3 cut(s)	873, 1416, 1758
BbvCI	CCTCAGC	1 cut(s)	254
BbvI	GCAGC	5 cut(s)	755, 758, 1196, 1490, 2365
BccI	CCATC	8 cut(s)	151, 866, 1025, 1271, 1495, 1767, 1774, 2150
BceAI	ACGGC	3 cut(s)	30, 734, 2184
BciT130I	CCWGG	4 cut(s)	87, 1061, 1331, 1868
BcnI	CCSGG	1 cut(s)	818
BcoDI	GTCTC	3 cut(s)	701, 1245, 1488
BfaI	CTAG	8 cut(s)	693, 740, 1296, 1356, 1491, 1724, 1838, 2285
BfmI	CTRYAG	1 cut(s)	1905
BfoI	RGCGCY	1 cut(s)	452
BfuAI	ACCTGC	1 cut(s)	236
BisI	GCNGC	9 cut(s)	18, 639, 744, 747, 1210, 1479, 1879, 2037, 2354
BlsI	GCNGC	9 cut(s)	19, 640, 745, 748, 1211, 1480, 1880, 2038, 2355
BmcAI	AGTACT	1 cut(s)	1597
Bme1390I	CCNGG	5 cut(s)	87, 818, 1061, 1331, 1868
Bme18I	GGWCC	4 cut(s)	953, 1056, 1893, 2289
BmeT110I	CYCGRG	2 cut(s)	97, 1913
BmgT120I	GGNCC	6 cut(s)	920, 953, 1056, 1893, 2159, 2289
BmiI	GGNNCC	4 cut(s)	450, 1058, 1820, 2290
BmrFI	CCNGG	5 cut(s)	87, 818, 1061, 1331, 1868
BmsI	GCATC	8 cut(s)	901, 999, 1171, 1380, 1465, 1680, 2068, 2318
BoxI	GACNNNNGTC	1 cut(s)	1298
BpiI	GAAGAC	3 cut(s)	323, 1716, 1809
BpmI	CTGGAG	1 cut(s)	167
Bpu10I	CCTNAGC	1 cut(s)	254
BpuEI	CTTGAG	1 cut(s)	608
BpuMI	CCSGG	1 cut(s)	818
BsaHI	GRCGYC	1 cut(s)	449
BsaJI	CCNNGG	7 cut(s)	218, 268, 816, 1059, 1876, 1929, 2082
BsaWI	WCCGGW	4 cut(s)	568, 787, 2278, 2314
Bsc4I	CCNNNNNNNGG	7 cut(s)	589, 698, 962, 1729, 1855, 2167, 2285
Bse118I	RCCGGY	3 cut(s)	242, 787, 1367
Bse1I	ACTGG	4 cut(s)	30, 433, 1140, 1593
Bse3DI	GCAATG	2 cut(s)	1044, 1296
BseAI	TCCGGA	1 cut(s)	2278
BseBI	CCWGG	4 cut(s)	87, 1061, 1331, 1868
BseDI	CCNNGG	7 cut(s)	218, 268, 816, 1059, 1876, 1929, 2082
BseGI	GGATG	4 cut(s)	861, 1107, 1919, 2367
BseLI	CCNNNNNNNGG	7 cut(s)	589, 698, 962, 1729, 1855, 2167, 2285
BseMI	GCAATG	2 cut(s)	1044, 1296
BseMII	CTCAG	7 cut(s)	42, 268, 330, 1401, 1429, 1620, 2177
BseNI	ACTGG	4 cut(s)	30, 433, 1140, 1593
BseRI	GAGGAG	3 cut(s)	605, 1542, 2156
BseSI	GKGCMC	1 cut(s)	276
BseXI	GCAGC	5 cut(s)	755, 758, 1196, 1490, 2365
BseYI	CCCAGC	2 cut(s)	946, 2241
BsgI	GTGCAG	2 cut(s)	1440, 2255
Bsh1236I	CGCG	1 cut(s)	1878
Bsh1285I	CGRYCG	1 cut(s)	788
BshFI	GGCC	7 cut(s)	20, 90, 829, 882, 921, 1881, 2160
BshNI	GGYRCC	1 cut(s)	448
BshTI	ACCGGT	1 cut(s)	787
BsiEI	CGRYCG	1 cut(s)	788
BsiHKAI	GWGCWC	3 cut(s)	873, 1416, 1758
BsiHKCI	CYCGRG	2 cut(s)	97, 1913
BsiSI	CCGG	9 cut(s)	243, 569, 788, 818, 879, 951, 1368, 2279, 2315
BslFI	GGGAC	5 cut(s)	929, 1042, 2224, 2302, 2312
BslI	CCNNNNNNNGG	7 cut(s)	589, 698, 962, 1729, 1855, 2167, 2285
BsmAI	GTCTC	3 cut(s)	701, 1245, 1488
BsmFI	GGGAC	5 cut(s)	929, 1042, 2224, 2302, 2312
BsmI	GAATGC	3 cut(s)	79, 1277, 1431
BsnI	GGCC	7 cut(s)	20, 90, 829, 882, 921, 1881, 2160
BsoBI	CYCGRG	2 cut(s)	97, 1913
Bsp1286I	GDGCHC	6 cut(s)	276, 357, 873, 1416, 1758, 1849
Bsp13I	TCCGGA	1 cut(s)	2278
Bsp143I	GATC	3 cut(s)	965, 1348, 2022
BspACI	CCGC	8 cut(s)	17, 639, 799, 1876, 1878, 1902, 2037, 2252
BspANI	GGCC	7 cut(s)	20, 90, 829, 882, 921, 1881, 2160
BspCNI	CTCAG	7 cut(s)	43, 267, 331, 1402, 1428, 1619, 2176
BspEI	TCCGGA	1 cut(s)	2278
BspFNI	CGCG	1 cut(s)	1878
BspHI	TCATGA	2 cut(s)	537, 712
BspLI	GGNNCC	4 cut(s)	450, 1058, 1820, 2290
BspMI	ACCTGC	1 cut(s)	236
BspPI	GGATC	3 cut(s)	973, 1343, 2017
BspQI	GCTCTTC	1 cut(s)	362
BspT107I	GGYRCC	1 cut(s)	448
BsrDI	GCAATG	2 cut(s)	1044, 1296
BsrFI	RCCGGY	3 cut(s)	242, 787, 1367
BsrI	ACTGG	4 cut(s)	30, 433, 1140, 1593
BssAI	RCCGGY	3 cut(s)	242, 787, 1367
BssECI	CCNNGG	7 cut(s)	218, 268, 816, 1059, 1876, 1929, 2082
BssMI	GATC	3 cut(s)	965, 1348, 2022
BssNAI	GTATAC	1 cut(s)	132
BssNI	GRCGYC	1 cut(s)	449
BssT1I	CCWWGG	2 cut(s)	218, 268
Bst1107I	GTATAC	1 cut(s)	132
Bst2UI	CCWGG	4 cut(s)	87, 1061, 1331, 1868
Bst4CI	ACNGT	6 cut(s)	522, 902, 1256, 1893, 2020, 2293
Bst6I	CTCTTC	1 cut(s)	362
BstACI	GRCGYC	1 cut(s)	449
BstAPI	GCANNNNNTGC	1 cut(s)	2233
BstC8I	GCNNGC	7 cut(s)	439, 580, 884, 1369, 1429, 1455, 2307
BstDEI	CTNAG	7 cut(s)	51, 254, 339, 1410, 1415, 1606, 2163
BstDSI	CCRYGG	2 cut(s)	1876, 1929
BstENI	CCTNNNNNAGG	1 cut(s)	587
BstF5I	GGATG	4 cut(s)	861, 1107, 1919, 2367
BstFNI	CGCG	1 cut(s)	1878
BstH2I	RGCGCY	1 cut(s)	452
BstHHI	GCGC	1 cut(s)	451
BstKTI	GATC	3 cut(s)	968, 1351, 2025
BstMAI	GTCTC	3 cut(s)	701, 1245, 1488
BstMBI	GATC	3 cut(s)	965, 1348, 2022
BstMCI	CGRYCG	1 cut(s)	788
BstMWI	GCNNNNNNNGC	6 cut(s)	476, 743, 947, 1215, 2233, 2242
BstNI	CCWGG	4 cut(s)	87, 1061, 1331, 1868
BstNSI	RCATGY	6 cut(s)	441, 634, 1171, 1453, 1485, 1705
BstPAI	GACNNNNGTC	1 cut(s)	1298
BstSCI	CCNGG	5 cut(s)	85, 816, 1059, 1329, 1866
BstSFI	CTRYAG	1 cut(s)	1905
BstSLI	GKGCMC	1 cut(s)	276
BstUI	CGCG	1 cut(s)	1878
BstV1I	GCAGC	5 cut(s)	755, 758, 1196, 1490, 2365
BstV2I	GAAGAC	3 cut(s)	323, 1716, 1809
BstXI	CCANNNNNNTGG	1 cut(s)	1990
BstZ17I	GTATAC	1 cut(s)	132
BsuRI	GGCC	7 cut(s)	20, 90, 829, 882, 921, 1881, 2160
BtgI	CCRYGG	2 cut(s)	1876, 1929
BtsCI	GGATG	4 cut(s)	861, 1107, 1919, 2367
BtsI	GCAGTG	1 cut(s)	1837
BtsIMutI	CAGTG	6 cut(s)	426, 655, 667, 1273, 1797, 1837
BveI	ACCTGC	1 cut(s)	236
Cac8I	GCNNGC	7 cut(s)	439, 580, 884, 1369, 1429, 1455, 2307
CciI	TCATGA	2 cut(s)	537, 712
CfoI	GCGC	1 cut(s)	451
Cfr10I	RCCGGY	3 cut(s)	242, 787, 1367
Cfr13I	GGNCC	6 cut(s)	920, 953, 1056, 1893, 2159, 2289
Cfr42I	CCGCGG	1 cut(s)	1879
CseI	GACGC	2 cut(s)	430, 1288
Csp6I	GTAC	6 cut(s)	57, 94, 1596, 2055, 2072, 2348
CspAI	ACCGGT	1 cut(s)	787
CspCI	CAANNNNNGTGG	2 cut(s)	2325, 2360
CviQI	GTAC	6 cut(s)	57, 94, 1596, 2055, 2072, 2348
DdeI	CTNAG	7 cut(s)	51, 254, 339, 1410, 1415, 1606, 2163
DinI	GGCGCC	1 cut(s)	450
DpnI	GATC	3 cut(s)	967, 1350, 2024
DpnII	GATC	3 cut(s)	965, 1348, 2022
EaeI	YGGCCR	2 cut(s)	18, 1879
Eam1104I	CTCTTC	1 cut(s)	362
EarI	CTCTTC	1 cut(s)	362
Ecl136II	GAGCTC	1 cut(s)	1414
Eco130I	CCWWGG	2 cut(s)	218, 268
Eco147I	AGGCCT	1 cut(s)	829
Eco24I	GRGCYC	3 cut(s)	357, 1416, 1849
Eco47I	GGWCC	4 cut(s)	953, 1056, 1893, 2289
Eco53kI	GAGCTC	1 cut(s)	1414
Eco57I	CTGAAG	1 cut(s)	2067
Eco88I	CYCGRG	2 cut(s)	97, 1913
EcoICRI	GAGCTC	1 cut(s)	1414
EcoNI	CCTNNNNNAGG	1 cut(s)	587
EcoRI	GAATTC	3 cut(s)	281, 607, 1405
EcoRII	CCWGG	4 cut(s)	85, 1059, 1329, 1866
EcoT14I	CCWWGG	2 cut(s)	218, 268
EcoT22I	ATGCAT	2 cut(s)	1395, 1707
EcoT38I	GRGCYC	3 cut(s)	357, 1416, 1849
EgeI	GGCGCC	1 cut(s)	450
EheI	GGCGCC	1 cut(s)	450
ErhI	CCWWGG	2 cut(s)	218, 268
FalI	AAGNNNNNCTT	2 cut(s)	281, 313
FaqI	GGGAC	5 cut(s)	929, 1042, 2224, 2302, 2312
FauI	CCCGC	1 cut(s)	2245
FauNDI	CATATG	2 cut(s)	1715, 2229
FblI	GTMKAC	2 cut(s)	131, 1872
Fnu4HI	GCNGC	9 cut(s)	18, 639, 744, 747, 1210, 1479, 1879, 2037, 2354
FokI	GGATG	3 cut(s)	848, 1094, 1906
FriOI	GRGCYC	3 cut(s)	357, 1416, 1849
Fsp4HI	GCNGC	9 cut(s)	18, 639, 744, 747, 1210, 1479, 1879, 2037, 2354
FspBI	CTAG	8 cut(s)	693, 740, 1296, 1356, 1491, 1724, 1838, 2285
GlaI	GCGC	1 cut(s)	450
GluI	GCNGC	9 cut(s)	18, 639, 744, 747, 1210, 1479, 1879, 2037, 2354
GsaI	CCCAGC	2 cut(s)	950, 2245
GsuI	CTGGAG	1 cut(s)	167
HaeII	RGCGCY	1 cut(s)	452
HaeIII	GGCC	7 cut(s)	20, 90, 829, 882, 921, 1881, 2160
HapII	CCGG	9 cut(s)	243, 569, 788, 818, 879, 951, 1368, 2279, 2315
HgaI	GACGC	2 cut(s)	430, 1288
HhaI	GCGC	1 cut(s)	451
Hin1I	GRCGYC	1 cut(s)	449
Hin6I	GCGC	1 cut(s)	449
HinP1I	GCGC	1 cut(s)	449
HpaII	CCGG	9 cut(s)	243, 569, 788, 818, 879, 951, 1368, 2279, 2315
HphI	GGTGA	7 cut(s)	75, 217, 1345, 1514, 1757, 2129, 2304
Hpy166II	GTNNAC	7 cut(s)	57, 132, 225, 310, 1873, 1896, 2072
Hpy8I	GTNNAC	7 cut(s)	57, 132, 225, 310, 1873, 1896, 2072
Hpy99I	CGWCG	3 cut(s)	446, 1118, 1181
HpyCH4III	ACNGT	6 cut(s)	522, 902, 1256, 1893, 2020, 2293
HpyCH4IV	ACGT	1 cut(s)	75
HpyF10VI	GCNNNNNNNGC	6 cut(s)	476, 743, 947, 1215, 2233, 2242
HpyF3I	CTNAG	7 cut(s)	51, 254, 339, 1410, 1415, 1606, 2163
HpySE526I	ACGT	1 cut(s)	75
Hsp92I	GRCGYC	1 cut(s)	449
HspAI	GCGC	1 cut(s)	449
KasI	GGCGCC	1 cut(s)	448
Kpn2I	TCCGGA	1 cut(s)	2278
KroI	GCCGGC	1 cut(s)	1367
KroNI	GCCGGC	1 cut(s)	1369
KspI	CCGCGG	1 cut(s)	1879
Kzo9I	GATC	3 cut(s)	965, 1348, 2022
LguI	GCTCTTC	1 cut(s)	362
LmnI	GCTCC	4 cut(s)	592, 876, 1761, 2281
Lsp1109I	GCAGC	5 cut(s)	755, 758, 1196, 1490, 2365
LweI	GCATC	8 cut(s)	901, 999, 1171, 1380, 1465, 1680, 2068, 2318
MaeI	CTAG	8 cut(s)	693, 740, 1296, 1356, 1491, 1724, 1838, 2285
MaeII	ACGT	1 cut(s)	75
MaeIII	GTNAC	7 cut(s)	46, 460, 546, 650, 1024, 1123, 1306
MalI	GATC	3 cut(s)	967, 1350, 2024
MboI	GATC	3 cut(s)	965, 1348, 2022
MboII	GAAGA	8 cut(s)	323, 349, 989, 1666, 1721, 1782, 1814, 1933
MfeI	CAATTG	2 cut(s)	1191, 2323
MhlI	GDGCHC	6 cut(s)	276, 357, 873, 1416, 1758, 1849
Mly113I	GGCGCC	1 cut(s)	449
MlyI	GAGTC	6 cut(s)	535, 553, 703, 1073, 1925, 1994
MmeI	TCCRAC	2 cut(s)	1840, 2050
Mph1103I	ATGCAT	2 cut(s)	1395, 1707
MroI	TCCGGA	1 cut(s)	2278
MroNI	GCCGGC	1 cut(s)	1367
MseI	TTAA	6 cut(s)	164, 303, 1073, 1095, 1247, 1667
MslI	CAYNNNNRTG	2 cut(s)	866, 1022
MspA1I	CMGCKG	3 cut(s)	127, 1878, 1904
MspI	CCGG	9 cut(s)	243, 569, 788, 818, 879, 951, 1368, 2279, 2315
MspR9I	CCNGG	5 cut(s)	87, 818, 1061, 1331, 1868
MunI	CAATTG	2 cut(s)	1191, 2323
Mva1269I	GAATGC	3 cut(s)	79, 1277, 1431
MvaI	CCWGG	4 cut(s)	87, 1061, 1331, 1868
MvnI	CGCG	1 cut(s)	1878
MwoI	GCNNNNNNNGC	6 cut(s)	476, 743, 947, 1215, 2233, 2242
NaeI	GCCGGC	1 cut(s)	1369
NarI	GGCGCC	1 cut(s)	449
NciI	CCSGG	1 cut(s)	818
NdeI	CATATG	2 cut(s)	1715, 2229
NdeII	GATC	3 cut(s)	965, 1348, 2022
NgoMIV	GCCGGC	1 cut(s)	1367
NlaIV	GGNNCC	4 cut(s)	450, 1058, 1820, 2290
NmeAIII	GCCGAG	1 cut(s)	2107
NmuCI	GTSAC	5 cut(s)	460, 650, 1024, 1123, 1306
NsiI	ATGCAT	2 cut(s)	1395, 1707
NspI	RCATGY	6 cut(s)	441, 634, 1171, 1453, 1485, 1705
PaeI	GCATGC	1 cut(s)	441
PaeR7I	CTCGAG	1 cut(s)	97
PagI	TCATGA	2 cut(s)	537, 712
PaqCI	CACCTGC	1 cut(s)	236
PceI	AGGCCT	1 cut(s)	829
PciSI	GCTCTTC	1 cut(s)	362
PctI	GAATGC	3 cut(s)	79, 1277, 1431
PdiI	GCCGGC	1 cut(s)	1369
PfeI	GAWTC	5 cut(s)	320, 1101, 1271, 1796, 1863
PflFI	GACNNNGTC	1 cut(s)	1613
PinAI	ACCGGT	1 cut(s)	787
PkrI	GCNGC	9 cut(s)	19, 640, 745, 748, 1211, 1480, 1880, 2038, 2355
PleI	GAGTC	6 cut(s)	535, 553, 703, 1073, 1924, 1994
PluTI	GGCGCC	1 cut(s)	452
PpsI	GAGTC	6 cut(s)	535, 553, 703, 1073, 1924, 1994
PshAI	GACNNNNGTC	1 cut(s)	1298
PshBI	ATTAAT	1 cut(s)	1095
PsiI	TTATAA	1 cut(s)	239
Psp124BI	GAGCTC	1 cut(s)	1416
Psp6I	CCWGG	4 cut(s)	85, 1059, 1329, 1866
PspFI	CCCAGC	2 cut(s)	946, 2241
PspGI	CCWGG	4 cut(s)	85, 1059, 1329, 1866
PspN4I	GGNNCC	4 cut(s)	450, 1058, 1820, 2290
PspPI	GGNCC	6 cut(s)	920, 953, 1056, 1893, 2159, 2289
PsyI	GACNNNGTC	1 cut(s)	1613
PvuII	CAGCTG	1 cut(s)	127
RsaI	GTAC	6 cut(s)	58, 95, 1597, 2056, 2073, 2349
RsaNI	GTAC	6 cut(s)	57, 94, 1596, 2055, 2072, 2348
RseI	CAYNNNNRTG	2 cut(s)	866, 1022
SacI	GAGCTC	1 cut(s)	1416
SacII	CCGCGG	1 cut(s)	1879
SapI	GCTCTTC	1 cut(s)	362
SaqAI	TTAA	6 cut(s)	164, 303, 1073, 1095, 1247, 1667
SatI	GCNGC	9 cut(s)	18, 639, 744, 747, 1210, 1479, 1879, 2037, 2354
Sau3AI	GATC	3 cut(s)	965, 1348, 2022
Sau96I	GGNCC	6 cut(s)	920, 953, 1056, 1893, 2159, 2289
ScaI	AGTACT	1 cut(s)	1597
SchI	GAGTC	6 cut(s)	535, 553, 703, 1073, 1925, 1994
ScrFI	CCNGG	5 cut(s)	87, 818, 1061, 1331, 1868
SduI	GDGCHC	6 cut(s)	276, 357, 873, 1416, 1758, 1849
SfaNI	GCATC	8 cut(s)	901, 999, 1171, 1380, 1465, 1680, 2068, 2318
SfcI	CTRYAG	1 cut(s)	1905
SfoI	GGCGCC	1 cut(s)	450
Sfr274I	CTCGAG	1 cut(s)	97
Sfr303I	CCGCGG	1 cut(s)	1879
SgrBI	CCGCGG	1 cut(s)	1879
SinI	GGWCC	4 cut(s)	953, 1056, 1893, 2289
SlaI	CTCGAG	1 cut(s)	97
SmiMI	CAYNNNNRTG	2 cut(s)	866, 1022
SmlI	CTYRAG	2 cut(s)	97, 587
SmoI	CTYRAG	2 cut(s)	97, 587
SphI	GCATGC	1 cut(s)	441
SseBI	AGGCCT	1 cut(s)	829
SsiI	CCGC	8 cut(s)	17, 639, 799, 1876, 1878, 1902, 2037, 2252
SspDI	GGCGCC	1 cut(s)	448
SspMI	CTAG	8 cut(s)	693, 740, 1296, 1356, 1491, 1724, 1838, 2285
SstI	GAGCTC	1 cut(s)	1416
StuI	AGGCCT	1 cut(s)	829
StyD4I	CCNGG	5 cut(s)	85, 816, 1059, 1329, 1866
StyI	CCWWGG	2 cut(s)	218, 268
TaaI	ACNGT	6 cut(s)	522, 902, 1256, 1893, 2020, 2293
TaiI	ACGT	1 cut(s)	78
TaqI	TCGA	5 cut(s)	98, 622, 784, 1179, 1778
TaqII	GACCGA	2 cut(s)	317, 2172
TatI	WGTACW	3 cut(s)	93, 1595, 2071
TauI	GCSGC	4 cut(s)	20, 641, 1881, 2039
TfiI	GAWTC	5 cut(s)	320, 1101, 1271, 1796, 1863
Tru1I	TTAA	6 cut(s)	164, 303, 1073, 1095, 1247, 1667
Tru9I	TTAA	6 cut(s)	164, 303, 1073, 1095, 1247, 1667
TscAI	CASTG	6 cut(s)	433, 655, 667, 1273, 1797, 1837
TseFI	GTSAC	5 cut(s)	460, 650, 1024, 1123, 1306
TseI	GCWGC	5 cut(s)	743, 746, 1209, 1478, 2353
Tsp45I	GTSAC	5 cut(s)	460, 650, 1024, 1123, 1306
TspDTI	ATGAA	9 cut(s)	349, 526, 1189, 1424, 1502, 1549, 1590, 1722, 2105
TspGWI	ACGGA	2 cut(s)	1918, 2072
TspRI	CASTG	6 cut(s)	433, 655, 667, 1273, 1797, 1837
Tth111I	GACNNNGTC	1 cut(s)	1613
VpaK11BI	GGWCC	4 cut(s)	953, 1056, 1893, 2289
VspI	ATTAAT	1 cut(s)	1095
XagI	CCTNNNNNAGG	1 cut(s)	587
XapI	RAATTY	8 cut(s)	281, 600, 607, 687, 1048, 1130, 1405, 2273
XbaI	TCTAGA	2 cut(s)	1355, 1490
XceI	RCATGY	6 cut(s)	441, 634, 1171, 1453, 1485, 1705
XhoI	CTCGAG	1 cut(s)	97
XmiI	GTMKAC	2 cut(s)	131, 1872
XspI	CTAG	8 cut(s)	693, 740, 1296, 1356, 1491, 1724, 1838, 2285
ZrmI	AGTACT	1 cut(s)	1597
Zsp2I	ATGCAT	2 cut(s)	1395, 1707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.