FvH4_3g43000

Belongs to the cystatin family. Phytocystatin subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002040
Physical Location & Seq
Reverse (-)
35919298 .. 35921194
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43000.t1

Sequence Viewer

Length: 666 bp
ATGGCAGCTGCTATTCGGTCTATGATTGGAAAGGTAGGAAATACAAGATCAGTAAGAAACGATGTTACATCTTTTCCAATTTCAAACCCTCATCTACACTTGGGTTATTCCTCTCGTCACCCTCTTGATCTATTTCCGGTAGTACACCACCCCCGCAATCAAAGGCTGCCAAAGAAATCAGAACCTGACAGCCGGCCGTCCATTCCAGAAATGCCATACCTTGGAACAGGTCCTATCAATGACTCCTCCAGAGATTGCCCAAGAGTTGTGATTAACGCACATTTCGCTTTGGAAGAGTTCAATAAGCAAAAGAATGCCCAACTGCAATTTGTAAGAGTGGTGAAGGCATATAACCAGGAGTGTGGTGGTGCTTTCAATCATCTAATGCTAACTTTGGAGGCTATAGACGCTAATAATGTGATCCAGATCTATCAAGCAATAGTGAAGCAGTTTTGGATTGCCAAACGTCCATTGCTGCTCCTATTATTTGGTCTCGTTGCCGATAATGGCAATTGGGATTTGATCAAACTTATTGACAACAGGGGTGTATTCGGACCAGGACCAACTGATAATCGATGTTTTGGTCGAAGGATAAAGATTCAAAAGACCCAGAGATGTTCCCTCGAGGAAGAGATTCTAAGCACCTTGCACTTGCCCCTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cystatin SQAPI

Protein Analysis

222

Amino Acids

24.98

Weight (kDa)

9.62

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 87 - 149 1.7e-08 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 221
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 415
AcoI YGGCCR 1 cut(s) 194
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 4 cut(s) 84, 301, 376, 602
AjnI CCWGG 2 cut(s) 354, 556
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 1 cut(s) 415
AlwNI CAGNNNCTG 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 623
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 4 cut(s) 5, 8, 166, 475
Asp700I GAANNNNTTC 1 cut(s) 633
AspS9I GGNCC 3 cut(s) 230, 554, 560
AsuHPI GGTGA 2 cut(s) 110, 352
AvaI CYCGRG 1 cut(s) 623
AvaII GGWCC 3 cut(s) 230, 554, 560
BbvI GCAGC 3 cut(s) 17, 153, 462
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 2 cut(s) 356, 558
BclI TGATCA 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 402
BglII AGATCT 1 cut(s) 426
BisI GCNGC 4 cut(s) 6, 9, 167, 476
BlsI GCNGC 4 cut(s) 7, 10, 168, 477
Bme1390I CCNGG 2 cut(s) 356, 558
Bme18I GGWCC 3 cut(s) 230, 554, 560
BmeT110I CYCGRG 1 cut(s) 623
BmgT120I GGNCC 3 cut(s) 230, 554, 560
BmrFI CCNGG 2 cut(s) 356, 558
BpmI CTGGAG 1 cut(s) 232
Bsa29I ATCGAT 1 cut(s) 574
BsaBI GATNNNNATC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 470
Bse8I GATNNNNATC 1 cut(s) 425
BseBI CCWGG 2 cut(s) 356, 558
BseCI ATCGAT 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 220
BseJI GATNNNNATC 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 235
BseX3I CGGCCG 1 cut(s) 194
BseXI GCAGC 3 cut(s) 17, 153, 462
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 574
BsiEI CGRYCG 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 623
BsiSI CCGG 2 cut(s) 137, 193
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 1 cut(s) 196
Bso31I GGTCTC 1 cut(s) 497
BsoBI CYCGRG 1 cut(s) 623
Bsp143I GATC 5 cut(s) 47, 127, 420, 426, 522
BspACI CCGC 1 cut(s) 154
BspANI GGCC 1 cut(s) 196
BspDI ATCGAT 1 cut(s) 574
BspPI GGATC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 5 cut(s) 47, 127, 420, 426, 522
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 356, 558
Bst6I CTCTTC 2 cut(s) 288, 624
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 638
BstKTI GATC 5 cut(s) 50, 130, 423, 429, 525
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 5 cut(s) 47, 127, 420, 426, 522
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 2 cut(s) 284, 407
BstNI CCWGG 2 cut(s) 356, 558
BstSCI CCNGG 2 cut(s) 354, 556
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 3 cut(s) 17, 153, 462
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 426
BstZI CGGCCG 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 574
BsuRI GGCC 1 cut(s) 196
BsuTUI ATCGAT 1 cut(s) 574
Cac8I GCNNGC 1 cut(s) 194
CaiI CAGNNNCTG 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 3 cut(s) 230, 554, 560
ClaI ATCGAT 1 cut(s) 574
CseI GACGC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 143
CviJI RGCY 5 cut(s) 8, 166, 192, 196, 401
CviKI_1 RGCY 5 cut(s) 8, 166, 192, 196, 401
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 1 cut(s) 638
DpnI GATC 5 cut(s) 49, 129, 422, 428, 524
DpnII GATC 5 cut(s) 47, 127, 420, 426, 522
EaeI YGGCCR 1 cut(s) 194
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 2 cut(s) 288, 624
EarI CTCTTC 2 cut(s) 288, 624
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 497
Eco47I GGWCC 3 cut(s) 230, 554, 560
Eco52I CGGCCG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 354, 556
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaiI YATR 5 cut(s) 23, 217, 349, 351, 404
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 4 cut(s) 6, 9, 167, 476
Fsp4HI GCNGC 4 cut(s) 6, 9, 167, 476
GluI GCNGC 4 cut(s) 6, 9, 167, 476
GsuI CTGGAG 1 cut(s) 232
HaeIII GGCC 1 cut(s) 196
HapII CCGG 2 cut(s) 137, 193
HgaI GACGC 1 cut(s) 416
HinfI GANTC 3 cut(s) 242, 598, 634
HpaII CCGG 2 cut(s) 137, 193
HphI GGTGA 2 cut(s) 110, 352
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 181, 554
Hpy188III TCNNGA 4 cut(s) 125, 206, 249, 424
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 337, 582
HpyCH4IV ACGT 1 cut(s) 466
HpyCH4V TGCA 2 cut(s) 325, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 407
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 1 cut(s) 466
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 5 cut(s) 47, 127, 420, 426, 522
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 3 cut(s) 17, 153, 462
MaeII ACGT 1 cut(s) 466
MaeIII GTNAC 2 cut(s) 64, 116
MalI GATC 5 cut(s) 49, 129, 422, 428, 524
MboI GATC 5 cut(s) 47, 127, 420, 426, 522
MboII GAAGA 2 cut(s) 305, 641
MfeI CAATTG 1 cut(s) 511
MflI RGATCY 1 cut(s) 426
MluCI AATT 3 cut(s) 78, 326, 511
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 7 cut(s) 99, 121, 132, 256, 391, 619, 632
MroNI GCCGGC 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 2 cut(s) 273, 660
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 137, 193
MspR9I CCNGG 2 cut(s) 356, 558
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 2 cut(s) 356, 558
MwoI GCNNNNNNNGC 2 cut(s) 284, 407
NaeI GCCGGC 1 cut(s) 194
NdeII GATC 5 cut(s) 47, 127, 420, 426, 522
NgoMIV GCCGGC 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 623
PctI GAATGC 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 598, 634
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 4 cut(s) 7, 10, 168, 477
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PpuMI RGGWCCY 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 354, 556
PspGI CCWGG 2 cut(s) 354, 556
PspPI GGNCC 3 cut(s) 230, 554, 560
PspPPI RGGWCCY 1 cut(s) 230
PspXI VCTCGAGB 1 cut(s) 623
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 273, 660
SatI GCNGC 4 cut(s) 6, 9, 167, 476
Sau3AI GATC 5 cut(s) 47, 127, 420, 426, 522
Sau96I GGNCC 3 cut(s) 230, 554, 560
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 2 cut(s) 356, 558
SetI ASST 7 cut(s) 10, 36, 187, 222, 232, 469, 647
SfcI CTRYAG 1 cut(s) 402
Sfr274I CTCGAG 1 cut(s) 623
SinI GGWCC 3 cut(s) 230, 554, 560
SlaI CTCGAG 1 cut(s) 623
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 3 cut(s) 78, 326, 511
SsiI CCGC 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 354, 556
StyI CCWWGG 1 cut(s) 220
TaiI ACGT 1 cut(s) 469
TaqI TCGA 3 cut(s) 574, 586, 624
TaqII GACCGA 1 cut(s) 6
TasI AATT 3 cut(s) 78, 326, 511
TatI WGTACW 1 cut(s) 142
TfiI GAWTC 2 cut(s) 598, 634
Tru1I TTAA 2 cut(s) 273, 660
Tru9I TTAA 2 cut(s) 273, 660
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 4 cut(s) 5, 8, 166, 475
Tsp45I GTSAC 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 230, 554, 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.