Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0002040

Physical Location & Seq

Genomic Coordinates Forward (+)

81560377 .. 81561720

Gene Structure 1344 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G047090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGGCAACGGCTATTCGATCTATGCTTGGAAATTTTGGGACTAGAAGATCCGTAGCAAACATGGTCATGCCTTTCGAAATCAGAACCCTAAATTTTAATCATGATCAGGAGGTGTTTCCAGTTTCGATGCCTCATCTAAATTTGGGCTATTCCTCTCGTCACCCTCTTCATTTTCTTTCTGCGTTACACTGTCCGTGCATTCAAAGGCGGCCTAAGAAAGTGAAACCTGACCACCAAGACATTCCAGAAATGTTATTTCTCGGAGCAAGTCCTATTGCTGCCTCATCCAGATATTGCATTAGAGTTGTGATTTATGCACATTTCGCTTTGGAAGAGTTCAACAAGCGCAAGAATTCCCAACTGCAATTTGTAAGAGTGGTAAAGGCATATAACCAGGTGTTTGGTGGTTGGACGGTTCGTTTGCTAACCTTGGAGGCAATTGATCCTGAGAATGTGATCCAGATTTACCAAGCAATAGTTAAGGAGCATTGCACTGGCAACCCTCCAGTGCAGCTCGAATTGTTTGGTCTTGTTGCTGAAAATGGCAATGGGAGTTTGTTGAAACTTATTGACAACAGGCGTTCAGGCCGAGGAGGACCAGTTGATAATCGGCGTGTCGGCCGAGAGATCTTAATGGTTCAAAAGAGAAAGAGATATCCTCTGGAGGCTGAGATACGAAGAACCCTGCACATCTTGCCCCTTAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

235

Amino Acids

26.87

Weight (kDa)

10.06

Isoelectric Point (pI)

53.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQAPI	PF16845	102 - 164	3.7e-10	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002040)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43000 FvH4_3g43000 FvH4_3g43020 FvH4_6g36050
malus_domestica	MD15G1382700.v1.1 MD17G1149600.v1.1
prunus_persica	Prupe.6G034500_v2.0.a1 Prupe.6G034700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0143281 RchiOBHm_Chr6g0253141
rosa_laevigata	RLG00000007620 RLG00000020020 RLG00000020024 RLG00000036651 RLG00000036657
rosa_multiflora	Rmu_sc0002560.1_g000017 Rmu_sc0003323.1_g000030 Rmu_sc0031119.1_g000001
rosa_roxburghii	Rroxscaffold_1G00005070 Rroxscaffold_1G00005080 Rroxscaffold_2G00101910 Rroxscaffold_4G00318690 Rroxscaffold_5G00364420
rosa_rugosa	Rorug05G0450800 Rorug05G0450900 Rorug05G0451300
rosa_samantha	Rh1DG126600 Rh2AG432800 Rh2CG418600 Rh2CG418900 Rh4AG238600 Rh4CG253700 Rh5AG505400 Rh5BG527400 Rh5CG550900 Rh5CG551600 Rh5DG539400 Rh5DG539500 Rh5DG539600 Rh6AG057200 Rh6BG051800 Rh6CG049700 Rh6DG045900
rosa_wichuraiana	Rw1G009800 Rw5G047090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	208
AclWI	GGATC	3 cut(s)	42, 437, 451
AcoI	YGGCCR	1 cut(s)	619
AcsI	RAATTY	4 cut(s)	31, 91, 139, 352
AgsI	TTSAA	4 cut(s)	203, 340, 562, 641
AjnI	CCWGG	1 cut(s)	393
AluBI	AGCT	1 cut(s)	514
AluI	AGCT	1 cut(s)	514
AlwI	GGATC	3 cut(s)	42, 437, 451
AoxI	GGCC	3 cut(s)	209, 586, 619
ApeKI	GCWGC	2 cut(s)	278, 511
ApoI	RAATTY	4 cut(s)	31, 91, 139, 352
ArsI	GACNNNNNNTTYG	2 cut(s)	403, 435
AspLEI	GCGC	1 cut(s)	348
AspS9I	GGNCC	1 cut(s)	596
AsuHPI	GGTGA	1 cut(s)	152
AsuII	TTCGAA	1 cut(s)	75
AvaII	GGWCC	1 cut(s)	596
BbvI	GCAGC	2 cut(s)	265, 523
BceAI	ACGGC	1 cut(s)	24
BciT130I	CCWGG	1 cut(s)	395
BclI	TGATCA	1 cut(s)	103
BfaI	CTAG	1 cut(s)	42
BglII	AGATCT	1 cut(s)	627
BisI	GCNGC	3 cut(s)	209, 279, 512
BlsI	GCNGC	3 cut(s)	210, 280, 513
Bme1390I	CCNGG	1 cut(s)	395
Bme18I	GGWCC	1 cut(s)	596
BmgT120I	GGNCC	1 cut(s)	596
BmrFI	CCNGG	1 cut(s)	395
BmsI	GCATC	1 cut(s)	117
BplI	GAGNNNNNCTC	2 cut(s)	643, 675
BpmI	CTGGAG	2 cut(s)	489, 683
Bpu14I	TTCGAA	1 cut(s)	75
BsaJI	CCNNGG	2 cut(s)	429, 589
BsaXI	ACNNNNNCTCC	2 cut(s)	585, 615
Bse1I	ACTGG	4 cut(s)	119, 499, 506, 599
Bse3DI	GCAATG	2 cut(s)	487, 553
BseBI	CCWGG	1 cut(s)	395
BseDI	CCNNGG	2 cut(s)	429, 589
BseGI	GGATG	1 cut(s)	284
BseMI	GCAATG	2 cut(s)	487, 553
BseMII	CTCAG	2 cut(s)	438, 660
BseNI	ACTGG	4 cut(s)	119, 499, 506, 599
BseRI	GAGGAG	1 cut(s)	606
BseX3I	CGGCCG	1 cut(s)	619
BseXI	GCAGC	2 cut(s)	265, 523
BsgI	GTGCAG	2 cut(s)	530, 671
Bsh1285I	CGRYCG	1 cut(s)	622
BshFI	GGCC	3 cut(s)	211, 588, 621
BsiEI	CGRYCG	1 cut(s)	622
BslFI	GGGAC	1 cut(s)	52
BsmFI	GGGAC	1 cut(s)	52
BsmI	GAATGC	1 cut(s)	198
BsnI	GGCC	3 cut(s)	211, 588, 621
Bsp119I	TTCGAA	1 cut(s)	75
Bsp143I	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
BspACI	CCGC	1 cut(s)	208
BspANI	GGCC	3 cut(s)	211, 588, 621
BspCNI	CTCAG	2 cut(s)	439, 661
BspHI	TCATGA	1 cut(s)	100
BspPI	GGATC	3 cut(s)	42, 437, 451
BspT104I	TTCGAA	1 cut(s)	75
BsrDI	GCAATG	2 cut(s)	487, 553
BsrI	ACTGG	4 cut(s)	119, 499, 506, 599
BssECI	CCNNGG	2 cut(s)	429, 589
BssMI	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
BssT1I	CCWWGG	1 cut(s)	429
Bst2UI	CCWGG	1 cut(s)	395
Bst4CI	ACNGT	2 cut(s)	191, 415
Bst6I	CTCTTC	2 cut(s)	171, 327
BstAPI	GCANNNNNTGC	1 cut(s)	694
BstBI	TTCGAA	1 cut(s)	75
BstDEI	CTNAG	3 cut(s)	213, 447, 669
BstF5I	GGATG	1 cut(s)	284
BstHHI	GCGC	1 cut(s)	348
BstKTI	GATC	6 cut(s)	20, 50, 106, 445, 459, 630
BstMBI	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
BstMCI	CGRYCG	1 cut(s)	622
BstMWI	GCNNNNNNNGC	2 cut(s)	323, 694
BstNI	CCWGG	1 cut(s)	395
BstSCI	CCNGG	1 cut(s)	393
BstV1I	GCAGC	2 cut(s)	265, 523
BstX2I	RGATCY	2 cut(s)	47, 627
BstXI	CCANNNNNNTGG	1 cut(s)	401
BstYI	RGATCY	2 cut(s)	47, 627
BstZI	CGGCCG	1 cut(s)	619
BsuRI	GGCC	3 cut(s)	211, 588, 621
BtsCI	GGATG	1 cut(s)	284
BtsIMutI	CAGTG	3 cut(s)	187, 492, 513
CciI	TCATGA	1 cut(s)	100
CfoI	GCGC	1 cut(s)	348
Cfr13I	GGNCC	1 cut(s)	596
CsiI	ACCWGGT	1 cut(s)	393
CviAII	CATG	3 cut(s)	61, 67, 101
CviJI	RGCY	7 cut(s)	11, 147, 211, 514, 588, 621, 668
CviKI_1	RGCY	7 cut(s)	11, 147, 211, 514, 588, 621, 668
DdeI	CTNAG	3 cut(s)	213, 447, 669
DpnI	GATC	6 cut(s)	19, 49, 105, 444, 458, 629
DpnII	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
EaeI	YGGCCR	1 cut(s)	619
EagI	CGGCCG	1 cut(s)	619
Eam1104I	CTCTTC	2 cut(s)	171, 327
EarI	CTCTTC	2 cut(s)	171, 327
EclXI	CGGCCG	1 cut(s)	619
Eco130I	CCWWGG	1 cut(s)	429
Eco32I	GATATC	1 cut(s)	656
Eco47I	GGWCC	1 cut(s)	596
Eco52I	CGGCCG	1 cut(s)	619
EcoRI	GAATTC	1 cut(s)	352
EcoRII	CCWGG	1 cut(s)	393
EcoRV	GATATC	1 cut(s)	656
EcoT14I	CCWWGG	1 cut(s)	429
ErhI	CCWWGG	1 cut(s)	429
FaeI	CATG	3 cut(s)	64, 70, 104
FaiI	YATR	7 cut(s)	23, 62, 68, 102, 315, 388, 390
FaqI	GGGAC	1 cut(s)	52
FatI	CATG	3 cut(s)	60, 66, 100
FbaI	TGATCA	1 cut(s)	103
Fnu4HI	GCNGC	3 cut(s)	209, 279, 512
FokI	GGATG	1 cut(s)	271
Fsp4HI	GCNGC	3 cut(s)	209, 279, 512
FspBI	CTAG	1 cut(s)	42
GlaI	GCGC	1 cut(s)	347
GluI	GCNGC	3 cut(s)	209, 279, 512
GsuI	CTGGAG	2 cut(s)	489, 683
HaeIII	GGCC	3 cut(s)	211, 588, 621
HhaI	GCGC	1 cut(s)	348
Hin1II	CATG	3 cut(s)	64, 70, 104
Hin6I	GCGC	1 cut(s)	346
HinP1I	GCGC	1 cut(s)	346
HphI	GGTGA	1 cut(s)	152
Hpy188I	TCNGA	2 cut(s)	83, 263
Hpy188III	TCNNGA	7 cut(s)	101, 107, 245, 288, 446, 460, 662
HpyCH4III	ACNGT	2 cut(s)	191, 415
HpyCH4V	TGCA	7 cut(s)	198, 297, 317, 364, 492, 511, 688
HpyF10VI	GCNNNNNNNGC	2 cut(s)	323, 694
HpyF3I	CTNAG	3 cut(s)	213, 447, 669
Hsp92II	CATG	3 cut(s)	64, 70, 104
HspAI	GCGC	1 cut(s)	346
Ksp22I	TGATCA	1 cut(s)	103
Kzo9I	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
LmnI	GCTCC	2 cut(s)	263, 484
Lsp1109I	GCAGC	2 cut(s)	265, 523
LweI	GCATC	1 cut(s)	117
MabI	ACCWGGT	1 cut(s)	393
MaeI	CTAG	1 cut(s)	42
MaeIII	GTNAC	2 cut(s)	158, 183
MalI	GATC	6 cut(s)	19, 49, 105, 444, 458, 629
MboI	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
MboII	GAAGA	4 cut(s)	57, 158, 344, 690
MfeI	CAATTG	1 cut(s)	438
MflI	RGATCY	2 cut(s)	47, 627
MluCI	AATT	7 cut(s)	31, 91, 139, 352, 365, 438, 518
MmeI	TCCRAC	1 cut(s)	389
MseI	TTAA	4 cut(s)	96, 480, 632, 702
MslI	CAYNNNNRTG	1 cut(s)	65
MspR9I	CCNGG	1 cut(s)	395
MunI	CAATTG	1 cut(s)	438
Mva1269I	GAATGC	1 cut(s)	198
MvaI	CCWGG	1 cut(s)	395
MwoI	GCNNNNNNNGC	2 cut(s)	323, 694
NdeII	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
NlaIII	CATG	3 cut(s)	64, 70, 104
NmeAIII	GCCGAG	2 cut(s)	614, 647
NmuCI	GTSAC	1 cut(s)	158
NspV	TTCGAA	1 cut(s)	75
PagI	TCATGA	1 cut(s)	100
PctI	GAATGC	1 cut(s)	198
PkrI	GCNGC	3 cut(s)	210, 280, 513
Psp6I	CCWGG	1 cut(s)	393
PspGI	CCWGG	1 cut(s)	393
PspPI	GGNCC	1 cut(s)	596
PsuI	RGATCY	2 cut(s)	47, 627
RseI	CAYNNNNRTG	1 cut(s)	65
SaqAI	TTAA	4 cut(s)	96, 480, 632, 702
SatI	GCNGC	3 cut(s)	209, 279, 512
Sau3AI	GATC	6 cut(s)	17, 47, 103, 442, 456, 627
Sau96I	GGNCC	1 cut(s)	596
ScrFI	CCNGG	1 cut(s)	395
SetI	ASST	5 cut(s)	114, 229, 399, 431, 516
SexAI	ACCWGGT	1 cut(s)	393
SfaNI	GCATC	1 cut(s)	117
SfuI	TTCGAA	1 cut(s)	75
SinI	GGWCC	1 cut(s)	596
SmiMI	CAYNNNNRTG	1 cut(s)	65
Sse9I	AATT	7 cut(s)	31, 91, 139, 352, 365, 438, 518
SsiI	CCGC	1 cut(s)	208
SspMI	CTAG	1 cut(s)	42
StyD4I	CCNGG	1 cut(s)	393
StyI	CCWWGG	1 cut(s)	429
TaaI	ACNGT	2 cut(s)	191, 415
TaqI	TCGA	4 cut(s)	16, 75, 125, 516
TasI	AATT	7 cut(s)	31, 91, 139, 352, 365, 438, 518
TauI	GCSGC	1 cut(s)	211
Tru1I	TTAA	4 cut(s)	96, 480, 632, 702
Tru9I	TTAA	4 cut(s)	96, 480, 632, 702
TscAI	CASTG	3 cut(s)	194, 499, 513
TseFI	GTSAC	1 cut(s)	158
TseI	GCWGC	2 cut(s)	278, 511
Tsp45I	GTSAC	1 cut(s)	158
TspDTI	ATGAA	1 cut(s)	158
TspGWI	ACGGA	2 cut(s)	40, 183
TspRI	CASTG	3 cut(s)	194, 499, 513
VpaK11BI	GGWCC	1 cut(s)	596
XapI	RAATTY	4 cut(s)	31, 91, 139, 352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	401
XspI	CTAG	1 cut(s)	42

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.