Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002040

Physical Location & Seq

Genomic Coordinates Forward (+)

47160393 .. 47161989

Gene Structure 1597 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1382700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGGCGGCAATGGCTTTCCGCTTTCCCTCTGTGGCTTTAAAGCTTGGGAGTTGGAGTAATAGTACTACGTTTCACCTTCATAGGATTACAAGAGAGAGAGAGGCACTACCAAGCACAGCTCCAACAGTATTATCCAACAACATTAACCAGCTCCAGCCACGTCCGGCCTCTTATCTAGACCATGAAAGCATACTTCCTAGAAGATTTACATGTCCCATCTCTGACGTTGGTAGATGTGCTCGTCATTACGAGAAACATGCTCTTTTCGCTGTCAAAGAGTTCAACAAGCAAAAGGATGCCCAACTGCAGTTTGTGAGGTGGGTAAGGGAAAGTCAGCAGCTTTTTTGCGGCTGTATGAACTATTTTATAACATTAGAGGCCGCTGATGCTGGTACGGTGAAGCTTTATCAAGCAATCGTTGCGCAGACAATCTCAAAGGAGCTCAGATTGTTTGGTCTTGTTCTTGATGGTGGAAGACTTTTAAAACTGATTGATAACAGGCGTACAAGCCCAAAGATCAATCGCACAAGAGATCCAATTGATACCAGAGAGCTTTACTACCCCGAGGTAAAAGTTCCAGTGAAGACAAGCAGATGTTCCCTTGAGGATGAGATGTTAAAAACCTTGCACGAATTGCCCCTCCAAAAATATTTAAGTTTTGGAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

223

Amino Acids

25.53

Weight (kDa)

9.53

Isoelectric Point (pI)

46.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQAPI	PF16845	84 - 147	1.1e-09	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002040)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43000 FvH4_3g43000 FvH4_3g43020 FvH4_6g36050
malus_domestica	MD15G1382700.v1.1 MD17G1149600.v1.1
prunus_persica	Prupe.6G034500_v2.0.a1 Prupe.6G034700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0143281 RchiOBHm_Chr6g0253141
rosa_laevigata	RLG00000007620 RLG00000020020 RLG00000020024 RLG00000036651 RLG00000036657
rosa_multiflora	Rmu_sc0002560.1_g000017 Rmu_sc0003323.1_g000030 Rmu_sc0031119.1_g000001
rosa_roxburghii	Rroxscaffold_1G00005070 Rroxscaffold_1G00005080 Rroxscaffold_2G00101910 Rroxscaffold_4G00318690 Rroxscaffold_5G00364420
rosa_rugosa	Rorug05G0450800 Rorug05G0450900 Rorug05G0451300
rosa_samantha	Rh1DG126600 Rh2AG432800 Rh2CG418600 Rh2CG418900 Rh4AG238600 Rh4CG253700 Rh5AG505400 Rh5BG527400 Rh5CG550900 Rh5CG551600 Rh5DG539400 Rh5DG539500 Rh5DG539600 Rh6AG057200 Rh6BG051800 Rh6CG049700 Rh6DG045900
rosa_wichuraiana	Rw1G009800 Rw5G047090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	368
Acc16I	TGCGCA	1 cut(s)	423
AciI	CCGC	4 cut(s)	5, 19, 348, 381
AclWI	GGATC	1 cut(s)	527
AcsI	RAATTY	1 cut(s)	663
AfaI	GTAC	3 cut(s)	64, 394, 505
AflIII	ACRYGT	1 cut(s)	209
AgsI	TTSAA	1 cut(s)	283
AjiI	CACGTC	1 cut(s)	161
AluBI	AGCT	7 cut(s)	43, 119, 151, 340, 403, 442, 553
AluI	AGCT	7 cut(s)	43, 119, 151, 340, 403, 442, 553
Alw21I	GWGCWC	2 cut(s)	241, 444
AlwI	GGATC	1 cut(s)	527
Ama87I	CYCGRG	1 cut(s)	563
AoxI	GGCC	2 cut(s)	165, 378
ApeKI	GCWGC	1 cut(s)	337
ApoI	RAATTY	1 cut(s)	663
AspLEI	GCGC	1 cut(s)	424
AsuHPI	GGTGA	2 cut(s)	65, 409
AvaI	CYCGRG	1 cut(s)	563
BanII	GRGCYC	1 cut(s)	444
BbsI	GAAGAC	2 cut(s)	481, 590
Bbv12I	GWGCWC	2 cut(s)	241, 444
BbvI	GCAGC	1 cut(s)	349
BccI	CCATC	2 cut(s)	224, 461
BcgI	CGANNNNNNTGC	2 cut(s)	239, 273
BfaI	CTAG	2 cut(s)	176, 198
BfmI	CTRYAG	1 cut(s)	305
BisI	GCNGC	4 cut(s)	6, 338, 349, 381
BlsI	GCNGC	4 cut(s)	7, 339, 350, 382
BmcAI	AGTACT	1 cut(s)	64
BmeT110I	CYCGRG	1 cut(s)	563
BmgBI	CACGTC	1 cut(s)	161
BmsI	GCATC	2 cut(s)	286, 376
BpiI	GAAGAC	2 cut(s)	481, 590
BpmI	CTGGAG	1 cut(s)	137
BpuEI	CTTGAG	1 cut(s)	623
BsaJI	CCNNGG	1 cut(s)	564
BsaXI	ACNNNNNCTCC	4 cut(s)	40, 46, 70, 76
Bse1I	ACTGG	1 cut(s)	578
Bse3DI	GCAATG	1 cut(s)	15
BseDI	CCNNGG	1 cut(s)	564
BseGI	GGATG	2 cut(s)	301, 613
BseMI	GCAATG	1 cut(s)	15
BseMII	CTCAG	1 cut(s)	457
BseNI	ACTGG	1 cut(s)	578
BseXI	GCAGC	1 cut(s)	349
BshFI	GGCC	2 cut(s)	167, 380
BsiHKAI	GWGCWC	2 cut(s)	241, 444
BsiHKCI	CYCGRG	1 cut(s)	563
BsiSI	CCGG	1 cut(s)	164
BslFI	GGGAC	1 cut(s)	198
BsmFI	GGGAC	1 cut(s)	198
BsnI	GGCC	2 cut(s)	167, 380
BsoBI	CYCGRG	1 cut(s)	563
Bsp1286I	GDGCHC	2 cut(s)	241, 444
Bsp143I	GATC	2 cut(s)	516, 532
BspACI	CCGC	4 cut(s)	5, 19, 348, 381
BspANI	GGCC	2 cut(s)	167, 380
BspCNI	CTCAG	1 cut(s)	456
BspMAI	CTGCAG	1 cut(s)	309
BspPI	GGATC	1 cut(s)	527
BsrDI	GCAATG	1 cut(s)	15
BsrI	ACTGG	1 cut(s)	578
BssECI	CCNNGG	1 cut(s)	564
BssMI	GATC	2 cut(s)	516, 532
Bst4CI	ACNGT	2 cut(s)	127, 397
BstAPI	GCANNNNNTGC	2 cut(s)	419, 634
BstDEI	CTNAG	1 cut(s)	443
BstF5I	GGATG	2 cut(s)	301, 613
BstHHI	GCGC	1 cut(s)	424
BstKTI	GATC	2 cut(s)	519, 535
BstMBI	GATC	2 cut(s)	516, 532
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 266, 386, 419, 634
BstNSI	RCATGY	2 cut(s)	213, 260
BstSFI	CTRYAG	1 cut(s)	305
BstV1I	GCAGC	1 cut(s)	349
BstV2I	GAAGAC	2 cut(s)	481, 590
BstX2I	RGATCY	1 cut(s)	532
BstYI	RGATCY	1 cut(s)	532
BsuRI	GGCC	2 cut(s)	167, 380
BtrI	CACGTC	1 cut(s)	161
BtsCI	GGATG	2 cut(s)	301, 613
BtsIMutI	CAGTG	1 cut(s)	585
CfoI	GCGC	1 cut(s)	424
Csp6I	GTAC	3 cut(s)	63, 393, 504
CviAII	CATG	3 cut(s)	182, 210, 257
CviQI	GTAC	3 cut(s)	63, 393, 504
DdeI	CTNAG	1 cut(s)	443
DpnI	GATC	2 cut(s)	518, 534
DpnII	GATC	2 cut(s)	516, 532
DraI	TTTAAA	2 cut(s)	39, 483
Ecl136II	GAGCTC	1 cut(s)	442
Eco24I	GRGCYC	1 cut(s)	444
Eco53kI	GAGCTC	1 cut(s)	442
Eco88I	CYCGRG	1 cut(s)	563
EcoICRI	GAGCTC	1 cut(s)	442
EcoT38I	GRGCYC	1 cut(s)	444
FaeI	CATG	3 cut(s)	185, 213, 260
FaiI	YATR	7 cut(s)	81, 183, 191, 211, 258, 356, 368
FaqI	GGGAC	1 cut(s)	198
FatI	CATG	3 cut(s)	181, 209, 256
Fnu4HI	GCNGC	4 cut(s)	6, 338, 349, 381
FokI	GGATG	2 cut(s)	308, 620
FriOI	GRGCYC	1 cut(s)	444
Fsp4HI	GCNGC	4 cut(s)	6, 338, 349, 381
FspBI	CTAG	2 cut(s)	176, 198
FspI	TGCGCA	1 cut(s)	423
GlaI	GCGC	1 cut(s)	423
GluI	GCNGC	4 cut(s)	6, 338, 349, 381
GsuI	CTGGAG	1 cut(s)	137
HaeIII	GGCC	2 cut(s)	167, 380
HapII	CCGG	1 cut(s)	164
HhaI	GCGC	1 cut(s)	424
Hin1II	CATG	3 cut(s)	185, 213, 260
Hin6I	GCGC	1 cut(s)	422
HinP1I	GCGC	1 cut(s)	422
HindIII	AAGCTT	2 cut(s)	41, 401
HpaII	CCGG	1 cut(s)	164
HphI	GGTGA	2 cut(s)	65, 409
Hpy188I	TCNGA	2 cut(s)	223, 446
Hpy188III	TCNNGA	2 cut(s)	176, 464
HpyAV	CCTTC	1 cut(s)	86
HpyCH4III	ACNGT	2 cut(s)	127, 397
HpyCH4IV	ACGT	3 cut(s)	68, 160, 225
HpyCH4V	TGCA	2 cut(s)	307, 628
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 266, 386, 419, 634
HpyF3I	CTNAG	1 cut(s)	443
HpySE526I	ACGT	3 cut(s)	68, 160, 225
Hsp92II	CATG	3 cut(s)	185, 213, 260
HspAI	GCGC	1 cut(s)	422
Kzo9I	GATC	2 cut(s)	516, 532
LmnI	GCTCC	3 cut(s)	124, 156, 439
LpnPI	CCDG	7 cut(s)	161, 167, 177, 375, 484, 559, 591
Lsp1109I	GCAGC	1 cut(s)	349
LweI	GCATC	2 cut(s)	286, 376
MaeI	CTAG	2 cut(s)	176, 198
MaeII	ACGT	3 cut(s)	68, 160, 225
MalI	GATC	2 cut(s)	518, 534
MboI	GATC	2 cut(s)	516, 532
MboII	GAAGA	3 cut(s)	213, 486, 595
MfeI	CAATTG	1 cut(s)	537
MflI	RGATCY	1 cut(s)	532
MhlI	GDGCHC	2 cut(s)	241, 444
MluCI	AATT	3 cut(s)	537, 632, 663
MmeI	TCCRAC	3 cut(s)	32, 146, 159
MnlI	CCTC	8 cut(s)	37, 94, 178, 309, 370, 559, 598, 650
MseI	TTAA	5 cut(s)	38, 144, 482, 617, 653
MspA1I	CMGCKG	1 cut(s)	383
MspI	CCGG	1 cut(s)	164
MunI	CAATTG	1 cut(s)	537
MwoI	GCNNNNNNNGC	5 cut(s)	11, 266, 386, 419, 634
NdeII	GATC	2 cut(s)	516, 532
NlaIII	CATG	3 cut(s)	185, 213, 260
NsbI	TGCGCA	1 cut(s)	423
NspI	RCATGY	2 cut(s)	213, 260
PciI	ACATGT	1 cut(s)	209
PkrI	GCNGC	4 cut(s)	7, 339, 350, 382
PscI	ACATGT	1 cut(s)	209
PsiI	TTATAA	1 cut(s)	368
Psp124BI	GAGCTC	1 cut(s)	444
PstI	CTGCAG	1 cut(s)	309
PsuI	RGATCY	1 cut(s)	532
RsaI	GTAC	3 cut(s)	64, 394, 505
RsaNI	GTAC	3 cut(s)	63, 393, 504
SacI	GAGCTC	1 cut(s)	444
SaqAI	TTAA	5 cut(s)	38, 144, 482, 617, 653
SatI	GCNGC	4 cut(s)	6, 338, 349, 381
Sau3AI	GATC	2 cut(s)	516, 532
ScaI	AGTACT	1 cut(s)	64
SduI	GDGCHC	2 cut(s)	241, 444
SfaNI	GCATC	2 cut(s)	286, 376
SfcI	CTRYAG	1 cut(s)	305
SmlI	CTYRAG	1 cut(s)	602
SmoI	CTYRAG	1 cut(s)	602
Sse9I	AATT	3 cut(s)	537, 632, 663
SsiI	CCGC	4 cut(s)	5, 19, 348, 381
SspI	AATATT	1 cut(s)	650
SspMI	CTAG	2 cut(s)	176, 198
SstI	GAGCTC	1 cut(s)	444
TaaI	ACNGT	2 cut(s)	127, 397
TaiI	ACGT	3 cut(s)	71, 163, 228
TasI	AATT	3 cut(s)	537, 632, 663
TatI	WGTACW	1 cut(s)	62
TauI	GCSGC	3 cut(s)	8, 351, 383
Tru1I	TTAA	5 cut(s)	38, 144, 482, 617, 653
Tru9I	TTAA	5 cut(s)	38, 144, 482, 617, 653
TscAI	CASTG	1 cut(s)	585
TseI	GCWGC	1 cut(s)	337
TspDTI	ATGAA	3 cut(s)	68, 198, 371
TspRI	CASTG	1 cut(s)	585
XapI	RAATTY	1 cut(s)	663
XbaI	TCTAGA	1 cut(s)	175
XceI	RCATGY	2 cut(s)	213, 260
XspI	CTAG	2 cut(s)	176, 198
ZrmI	AGTACT	1 cut(s)	64

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.