RchiOBHm_Chr2g0143281

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002040
Physical Location & Seq
Forward (+)
60982702 .. 60984340
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51338

Sequence Viewer

Length: 669 bp
ATGGCTTTTCGGTCTGTGCTTGCAAAGCTGGGGAATAATTGTGTTAAAGGGAGTGAAAGAAGAAGATTGGTTGCGGCCTTTGACACCAGAACCCTAGTCAAGTATCATAACATTTGGGATGATGATGAATACCAACGGCTGATGAACGATCGCATTGAACTTTTACGTGAAGCTGGTCCTTGGCGGAGGCGTGCTCGTGAACGTTTTCGTGTTCCGCCACATCGTCAACCTACGGATAATCCCAACGTTATGGCTTATGCCCATTTGGCCGTGGAAGAGTTCAACAAACAAAAGAATGCACAACTTCAATTTGTGAGAGCGGTGAAGGCATATTGCCAGGAGTACGATGATACCTGGGGAGATAAGTACTATTACCTAACGTTGGAGGCAGTTGATGCTCAAGTTGTTAAAACATACCAAGCAGTAGTTCATGACTCTACTGAGCCATCGCTCCACATTTTTGGTCTTGTTACTGATAATGGTTTGTTGCAACTAACTGATAACCATGTTCGTCCTTCACTTTATTGCACACTTGACGACCGAGATGTTCGACGCAAGGGAAGAGGATATTCTGATGAAACAGAGATAGAGGTCACGCTGCAGACAAGGGGAGGATATTTTCTCCAGGATGAGATACTTGGCTCGTTGCACGGACTTCCCTCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.87

Weight (kDa)

6.66

Isoelectric Point (pI)

57.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 79 - 146 9.9e-09 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 320
AciI CCGC 4 cut(s) 74, 184, 215, 320
AclI AACGTT 3 cut(s) 202, 246, 380
AcoI YGGCCR 1 cut(s) 267
AfaI GTAC 2 cut(s) 344, 368
AfiI CCNNNNNNNGG 1 cut(s) 382
AgsI TTSAA 3 cut(s) 158, 283, 308
AjnI CCWGG 3 cut(s) 336, 353, 624
AjuI GAANNNNNNNTTGG 2 cut(s) 411, 443
AluBI AGCT 2 cut(s) 28, 173
AluI AGCT 2 cut(s) 28, 173
Alw21I GWGCWC 1 cut(s) 196
AoxI GGCC 2 cut(s) 75, 267
ApeKI GCWGC 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 334
AvaII GGWCC 1 cut(s) 176
BauI CACGAG 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 585
BccI CCATC 1 cut(s) 454
BceAI ACGGC 2 cut(s) 152, 254
BciT130I CCWGG 3 cut(s) 338, 355, 626
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 599
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 2 cut(s) 75, 599
BlsI GCNGC 2 cut(s) 76, 600
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 3 cut(s) 338, 355, 626
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmrFI CCNGG 3 cut(s) 338, 355, 626
BmsI GCATC 1 cut(s) 385
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 608
BpuEI CTTGAG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 179, 270, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 382
BseBI CCWGG 3 cut(s) 338, 355, 626
BseDI CCNNGG 3 cut(s) 179, 270, 354
BseGI GGATG 2 cut(s) 124, 634
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 432
BseXI GCAGC 1 cut(s) 585
BseYI CCCAGC 1 cut(s) 28
Bsh1285I CGRYCG 2 cut(s) 151, 541
BshFI GGCC 2 cut(s) 77, 269
BsiEI CGRYCG 2 cut(s) 151, 541
BsiHKAI GWGCWC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 382
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 2 cut(s) 77, 269
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 4 cut(s) 74, 184, 215, 320
BspANI GGCC 2 cut(s) 77, 269
BspCNI CTCAG 1 cut(s) 433
BspHI TCATGA 1 cut(s) 430
BspMAI CTGCAG 1 cut(s) 603
BsrBI CCGCTC 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 179, 270, 354
BssMI GATC 1 cut(s) 148
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 338, 355, 626
Bst6I CTCTTC 2 cut(s) 270, 556
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 21, 192
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 124, 634
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMCI CGRYCG 2 cut(s) 151, 541
BstMWI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
BstNI CCWGG 3 cut(s) 338, 355, 626
BstSCI CCNGG 3 cut(s) 336, 353, 624
BstSFI CTRYAG 1 cut(s) 599
BstV1I GCAGC 1 cut(s) 585
BstXI CCANNNNNNTGG 2 cut(s) 250, 461
BsuRI GGCC 2 cut(s) 77, 269
BtgI CCRYGG 1 cut(s) 270
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 124, 634
Cac8I GCNNGC 2 cut(s) 21, 192
CciI TCATGA 1 cut(s) 430
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 343, 367
CviAII CATG 2 cut(s) 431, 506
CviJI RGCY 9 cut(s) 5, 28, 77, 139, 173, 254, 269, 445, 642
CviKI_1 RGCY 9 cut(s) 5, 28, 77, 139, 173, 254, 269, 445, 642
CviQI GTAC 2 cut(s) 343, 367
DdeI CTNAG 1 cut(s) 441
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 270, 556
EarI CTCTTC 2 cut(s) 270, 556
EciI GGCGGA 2 cut(s) 199, 204
Eco130I CCWWGG 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 176
EcoRII CCWGG 3 cut(s) 336, 353, 624
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 2 cut(s) 434, 509
FaiI YATR 7 cut(s) 108, 251, 258, 331, 415, 432, 507
FatI CATG 2 cut(s) 430, 505
Fnu4HI GCNGC 2 cut(s) 75, 599
FokI GGATG 2 cut(s) 131, 641
Fsp4HI GCNGC 2 cut(s) 75, 599
FspBI CTAG 1 cut(s) 95
GluI GCNGC 2 cut(s) 75, 599
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 2 cut(s) 77, 269
HgaI GACGC 1 cut(s) 561
Hin1II CATG 2 cut(s) 434, 509
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HinfI GANTC 1 cut(s) 434
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 200, 227
Hpy188I TCNGA 1 cut(s) 574
Hpy188III TCNNGA 2 cut(s) 197, 431
Hpy8I GTNNAC 2 cut(s) 200, 227
Hpy99I CGWCG 1 cut(s) 555
HpyAV CCTTC 2 cut(s) 319, 525
HpyCH4IV ACGT 4 cut(s) 166, 202, 246, 380
HpyCH4V TGCA 6 cut(s) 23, 299, 490, 528, 601, 649
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 4 cut(s) 166, 202, 246, 380
Hsp92II CATG 2 cut(s) 434, 509
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 1 cut(s) 456
LpnPI CCDG 9 cut(s) 14, 100, 159, 323, 340, 350, 367, 611, 638
Lsp1109I GCAGC 1 cut(s) 585
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 95
MaeII ACGT 4 cut(s) 166, 202, 246, 380
MaeIII GTNAC 2 cut(s) 469, 592
MalI GATC 1 cut(s) 150
MbiI CCGCTC 1 cut(s) 320
MboI GATC 1 cut(s) 148
MboII GAAGA 4 cut(s) 72, 75, 287, 573
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 2 cut(s) 37, 308
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 5 cut(s) 180, 379, 557, 583, 605
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 2 cut(s) 45, 408
MspR9I CCNGG 3 cut(s) 338, 355, 626
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 3 cut(s) 338, 355, 626
MwoI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
NdeII GATC 1 cut(s) 148
NlaIII CATG 2 cut(s) 434, 509
NmuCI GTSAC 1 cut(s) 592
PagI TCATGA 1 cut(s) 430
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 624
PkrI GCNGC 2 cut(s) 76, 600
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 167
Psp1406I AACGTT 3 cut(s) 202, 246, 380
Psp6I CCWGG 3 cut(s) 336, 353, 624
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 3 cut(s) 336, 353, 624
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 603
PvuI CGATCG 1 cut(s) 151
RsaI GTAC 2 cut(s) 344, 368
RsaNI GTAC 2 cut(s) 343, 367
SaqAI TTAA 2 cut(s) 45, 408
SatI GCNGC 2 cut(s) 75, 599
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 1 cut(s) 176
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 3 cut(s) 338, 355, 626
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 599
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
Sse9I AATT 2 cut(s) 37, 308
SsiI CCGC 4 cut(s) 74, 184, 215, 320
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 336, 353, 624
StyI CCWWGG 1 cut(s) 179
TaiI ACGT 4 cut(s) 169, 205, 249, 383
TaqI TCGA 1 cut(s) 550
TaqII GACCGA 1 cut(s) 555
TasI AATT 2 cut(s) 37, 308
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 2 cut(s) 45, 408
Tru9I TTAA 2 cut(s) 45, 408
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 1 cut(s) 598
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 4 cut(s) 141, 158, 419, 591
TspGWI ACGGA 2 cut(s) 248, 666
VpaK11BI GGWCC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 95
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.