Rh4AG238600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002040
Physical Location & Seq
Forward (+)
54445103 .. 54447922
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG238600.1

Sequence Viewer

Length: 726 bp
ATGGCAGACAAGGTTAGTGGAATTGGAGCCTCAAACAATCATTCGATGCCCCCTTATTTTGGAATTAGGCACCAAGAACACAAACCCTTGTTCCCGCCTTTCATTCGAAGCCTCATGCAGAAAGGATCATTAACAAACGCAGAATTAACTCGCATTCAAACCTTCCTGGGGGAATTAAACTATCAAGGCTCGTGGACTCCCGACGAACAATTAACCTACCAAGAATTGTCGAATCGGCTGGTGCTGCTTAATGTAACGTTAAACGCACGCGAATCAATCCTTTGGAGACTGAGGGATGATGATCCCAAGATTACAGCTACTGCTGGTTTTGCGGTGGCAGAATTCAACAAGCAGAAGAATGCCCAGCTGCGATTCATTAGAGCAGTAAAGGGATTTCGCAGTTATAGTCGTCTTTGGTTTGATTACTTTCTAACTTTGGAGGCAGTTGATGATCATCTGGGCAGAACTTACCAAGCAGTAGTTAAAGAAAATCTCGTGTATCCCCATAAACCCTGTAAGGTCCTATTATTTGGTCTTGTTGCTGATAACGGCAATGGGAATTTGTTAAATCTAATTGATAACAGATGGAGTGGGCCTTATGGTGAATGTCTATATGATGATCGAGGTTTTACATTCAGAGTTGGGGATTACAAGTTAAAGCCTCCAATGAAAATAAAAAGATGTTCTCTTGAGGGTGAGATACTAAGCTCCTTGCACATGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.63

Weight (kDa)

9.05

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 100 - 166 1.4e-08 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AccII CGCG 1 cut(s) 270
AciI CCGC 2 cut(s) 95, 332
AclI AACGTT 1 cut(s) 257
AclWI GGATC 2 cut(s) 133, 296
AcsI RAATTY 2 cut(s) 341, 559
AfiI CCNNNNNNNGG 2 cut(s) 59, 168
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 2 cut(s) 158, 346
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 3 cut(s) 317, 367, 708
AluI AGCT 3 cut(s) 317, 367, 708
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 133, 296
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 593
ApeKI GCWGC 2 cut(s) 244, 367
ApoI RAATTY 2 cut(s) 341, 559
AspS9I GGNCC 2 cut(s) 520, 593
AsuHPI GGTGA 2 cut(s) 614, 707
AsuII TTCGAA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 69
BauI CACGAG 2 cut(s) 190, 494
BbvI GCAGC 2 cut(s) 231, 354
BccI CCATC 1 cut(s) 579
BceAI ACGGC 1 cut(s) 565
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 510
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 280
BfuI GTATCC 1 cut(s) 510
BisI GCNGC 2 cut(s) 245, 368
BlsI GCNGC 2 cut(s) 246, 369
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 520
BmgT120I GGNCC 2 cut(s) 520, 593
BmiI GGNNCC 2 cut(s) 28, 71
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 710
BsaBI GATNNNNATC 2 cut(s) 300, 453
BsaJI CCNNGG 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 168
Bse3DI GCAATG 1 cut(s) 559
Bse8I GATNNNNATC 2 cut(s) 300, 453
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 2 cut(s) 300, 453
BseLI CCNNNNNNNGG 2 cut(s) 59, 168
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 281
BseXI GCAGC 2 cut(s) 231, 354
BseYI CCCAGC 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 595
BshNI GGYRCC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 59, 168
BsmAI GTCTC 1 cut(s) 280
BsmI GAATGC 2 cut(s) 153, 364
BsnI GGCC 1 cut(s) 595
Bsp119I TTCGAA 1 cut(s) 106
Bsp143I GATC 4 cut(s) 125, 301, 451, 619
BspACI CCGC 2 cut(s) 95, 332
BspANI GGCC 1 cut(s) 595
BspCNI CTCAG 1 cut(s) 282
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 2 cut(s) 28, 71
BspPI GGATC 2 cut(s) 133, 296
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 559
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 4 cut(s) 125, 301, 451, 619
BssSI CACGAG 2 cut(s) 190, 494
Bst2BI CACGAG 2 cut(s) 190, 494
Bst2UI CCWGG 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 2 cut(s) 290, 704
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 4 cut(s) 128, 304, 454, 622
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 125, 301, 451, 619
BstMWI GCNNNNNNNGC 2 cut(s) 244, 329
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 1 cut(s) 165
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 231, 354
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 1 cut(s) 595
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 520, 593
CspCI CAANNNNNGTGG 3 cut(s) 33, 173, 208
CviAII CATG 2 cut(s) 115, 718
CviJI RGCY 9 cut(s) 29, 111, 189, 238, 317, 367, 595, 661, 708
CviKI_1 RGCY 9 cut(s) 29, 111, 189, 238, 317, 367, 595, 661, 708
DdeI CTNAG 2 cut(s) 290, 704
DpnI GATC 4 cut(s) 127, 303, 453, 621
DpnII GATC 4 cut(s) 125, 301, 451, 619
Eco47I GGWCC 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 118, 721
FaiI YATR 7 cut(s) 116, 405, 507, 600, 613, 615, 719
FatI CATG 2 cut(s) 114, 717
FauI CCCGC 1 cut(s) 102
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 245, 368
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 245, 368
GluI GCNGC 2 cut(s) 245, 368
GsaI CCCAGC 1 cut(s) 367
HaeIII GGCC 1 cut(s) 595
Hin1II CATG 2 cut(s) 118, 721
HinfI GANTC 4 cut(s) 196, 232, 272, 372
HphI GGTGA 2 cut(s) 614, 707
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 1 cut(s) 638
Hpy188III TCNNGA 2 cut(s) 200, 689
Hpy8I GTNNAC 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 2 cut(s) 118, 715
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 329
HpyF3I CTNAG 2 cut(s) 290, 704
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 118, 721
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 125, 301, 451, 619
LmnI GCTCC 2 cut(s) 26, 713
LpnPI CCDG 7 cut(s) 152, 179, 224, 309, 377, 443, 526
Lsp1109I GCAGC 2 cut(s) 231, 354
LweI GCATC 1 cut(s) 36
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 253
MalI GATC 4 cut(s) 127, 303, 453, 621
MboI GATC 4 cut(s) 125, 301, 451, 619
MboII GAAGA 1 cut(s) 367
MluCI AATT 9 cut(s) 21, 63, 143, 173, 209, 224, 341, 559, 573
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 7 cut(s) 40, 122, 285, 433, 617, 672, 685
MseI TTAA 9 cut(s) 131, 146, 176, 212, 249, 260, 483, 566, 656
MspA1I CMGCKG 1 cut(s) 367
MspR9I CCNGG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 153, 364
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 244, 329
NdeII GATC 4 cut(s) 125, 301, 451, 619
NlaIII CATG 2 cut(s) 118, 721
NlaIV GGNNCC 2 cut(s) 28, 71
NspI RCATGY 1 cut(s) 721
NspV TTCGAA 1 cut(s) 106
PciI ACATGT 1 cut(s) 717
PctI GAATGC 2 cut(s) 153, 364
PfeI GAWTC 3 cut(s) 232, 272, 372
PkrI GCNGC 2 cut(s) 246, 369
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 520
PscI ACATGT 1 cut(s) 717
Psp1406I AACGTT 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 363
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 28, 71
PspPI GGNCC 2 cut(s) 520, 593
PspPPI RGGWCCY 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 367
SaqAI TTAA 9 cut(s) 131, 146, 176, 212, 249, 260, 483, 566, 656
SatI GCNGC 2 cut(s) 245, 368
Sau3AI GATC 4 cut(s) 125, 301, 451, 619
Sau96I GGNCC 2 cut(s) 520, 593
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 167
SetI ASST 9 cut(s) 15, 164, 218, 260, 319, 369, 522, 628, 710
SfaNI GCATC 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 9 cut(s) 21, 63, 143, 173, 209, 224, 341, 559, 573
SsiI CCGC 2 cut(s) 95, 332
StyD4I CCNGG 1 cut(s) 165
TaiI ACGT 1 cut(s) 260
TaqI TCGA 4 cut(s) 44, 106, 230, 622
TasI AATT 9 cut(s) 21, 63, 143, 173, 209, 224, 341, 559, 573
TfiI GAWTC 3 cut(s) 232, 272, 372
Tru1I TTAA 9 cut(s) 131, 146, 176, 212, 249, 260, 483, 566, 656
Tru9I TTAA 9 cut(s) 131, 146, 176, 212, 249, 260, 483, 566, 656
TseI GCWGC 2 cut(s) 244, 367
TspDTI ATGAA 3 cut(s) 91, 364, 683
VpaK11BI GGWCC 1 cut(s) 520
XapI RAATTY 2 cut(s) 341, 559
XceI RCATGY 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.