Rh2AG432800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002040
Physical Location & Seq
Forward (+)
64225538 .. 64238587
13050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG432800.1

Sequence Viewer

Length: 798 bp
ATGGCTTTTCGGTCTGTGCTTGCAAAGCTGGGGAATAATTGTGTTAAAGGGAGTGAAAGAAGAAGATTGGTTGCGGCCTTTGACACCAGAACCCTACTCAAGTATCATAACATTTGGGATGATGATGAATACCAACAGCTGATGAACGATCGCATTGAACTTCAACGTGAAACTGGTCCTTGGCGGAGGCGTGCTCGTGAACGTTTTCGTGTTCCGCCACATCGTCAACCTACGGATAATCCCAACGTTATGGCTTATGCCCATTTGGCCGTGGAAGAGTTCAACAAACAAAAGAATGCACAACTTCAATTTGTGAGAGCGGTGAAGGCATATTGCCAGGAGTACGATGATACCTGGGGAGATAAGTACTATTACCTAACGTTGGAGGCAGTTGATGCTCAAGTTGTTAAAACATACCAAGCAGTAGTTCATGACTCTACTGAGCCATCGCTCCACATTTTTGGTCTTGTTACTGATAATGGTTTGTTGCAACTAACTGATAACCATGTTCTTCCTTCACTTTATTGCACACTTGACGACCGAGCTGTGTATTTACTCAAATGTATTGAAGCATCAATCAAGTTGGGTTGGTTAAGCCCTATTGAATATACAGACTTACACTTTGGATTGAATTGGGTTTTGGATCTATTGCAGCAACAAGAAAATTGGGTCTTGGATCTTTCCTGTAGAGAAGGTGATGGTTGGGGTATGTCCAGAGAAGGAAACAAGAGGATGGGGGAAGAGTTGATCCACTGCTTTTGTGAAGGAGTTGAGTTTTTGGAGTCTTTTAATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

30.94

Weight (kDa)

5.42

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 79 - 146 1.4e-08 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 320
AciI CCGC 4 cut(s) 74, 184, 215, 320
AclI AACGTT 3 cut(s) 202, 246, 380
AclWI GGATC 3 cut(s) 651, 684, 742
AcoI YGGCCR 1 cut(s) 267
AfaI GTAC 2 cut(s) 344, 368
AfiI CCNNNNNNNGG 1 cut(s) 382
AgsI TTSAA 7 cut(s) 158, 164, 283, 308, 569, 605, 631
AjnI CCWGG 2 cut(s) 336, 353
AjuI GAANNNNNNNTTGG 4 cut(s) 411, 443, 623, 655
AluBI AGCT 3 cut(s) 28, 139, 545
AluI AGCT 3 cut(s) 28, 139, 545
Alw21I GWGCWC 1 cut(s) 196
AlwI GGATC 3 cut(s) 651, 684, 742
AoxI GGCC 2 cut(s) 75, 267
ApeKI GCWGC 1 cut(s) 652
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
Asp700I GAANNNNTTC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 334, 707
AvaII GGWCC 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 86, 119
BauI CACGAG 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 664
BccI CCATC 3 cut(s) 454, 692, 727
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 2 cut(s) 338, 355
BfmI CTRYAG 1 cut(s) 685
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 2 cut(s) 75, 653
BlsI GCNGC 2 cut(s) 76, 654
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 338, 355
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmrFI CCNGG 2 cut(s) 338, 355
BmsI GCATC 2 cut(s) 385, 581
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpuEI CTTGAG 2 cut(s) 83, 384
BsaJI CCNNGG 3 cut(s) 179, 270, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 2 cut(s) 338, 355
BseDI CCNNGG 3 cut(s) 179, 270, 354
BseGI GGATG 2 cut(s) 124, 738
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 664
BseYI CCCAGC 1 cut(s) 28
Bsh1285I CGRYCG 2 cut(s) 151, 541
BshFI GGCC 2 cut(s) 77, 269
BsiEI CGRYCG 2 cut(s) 151, 541
BsiHKAI GWGCWC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 382
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 2 cut(s) 77, 269
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 4 cut(s) 148, 643, 676, 747
BspACI CCGC 4 cut(s) 74, 184, 215, 320
BspANI GGCC 2 cut(s) 77, 269
BspCNI CTCAG 1 cut(s) 433
BspHI TCATGA 1 cut(s) 430
BspPI GGATC 3 cut(s) 651, 684, 742
BsrBI CCGCTC 1 cut(s) 320
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 179, 270, 354
BssMI GATC 4 cut(s) 148, 643, 676, 747
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 338, 355
Bst6I CTCTTC 2 cut(s) 270, 735
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 21, 192
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 124, 738
BstKTI GATC 4 cut(s) 151, 646, 679, 750
BstMBI GATC 4 cut(s) 148, 643, 676, 747
BstMCI CGRYCG 2 cut(s) 151, 541
BstMWI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
BstNI CCWGG 2 cut(s) 338, 355
BstSCI CCNGG 2 cut(s) 336, 353
BstSFI CTRYAG 1 cut(s) 685
BstV1I GCAGC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 643, 676
BstXI CCANNNNNNTGG 2 cut(s) 250, 461
BstYI RGATCY 2 cut(s) 643, 676
BsuRI GGCC 2 cut(s) 77, 269
BtgI CCRYGG 1 cut(s) 270
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 124, 738
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 1 cut(s) 751
Cac8I GCNNGC 2 cut(s) 21, 192
CciI TCATGA 1 cut(s) 430
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 343, 367
CspCI CAANNNNNGTGG 2 cut(s) 740, 775
CviAII CATG 2 cut(s) 431, 506
CviJI RGCY 9 cut(s) 5, 28, 77, 139, 254, 269, 445, 545, 597
CviKI_1 RGCY 9 cut(s) 5, 28, 77, 139, 254, 269, 445, 545, 597
CviQI GTAC 2 cut(s) 343, 367
DdeI CTNAG 1 cut(s) 441
DpnI GATC 4 cut(s) 150, 645, 678, 749
DpnII GATC 4 cut(s) 148, 643, 676, 747
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 270, 735
EarI CTCTTC 2 cut(s) 270, 735
EciI GGCGGA 2 cut(s) 199, 204
Eco130I CCWWGG 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 336, 353
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 2 cut(s) 434, 509
FaiI YATR 9 cut(s) 108, 251, 258, 331, 415, 432, 507, 609, 710
FatI CATG 2 cut(s) 430, 505
Fnu4HI GCNGC 2 cut(s) 75, 653
FokI GGATG 2 cut(s) 131, 745
Fsp4HI GCNGC 2 cut(s) 75, 653
GluI GCNGC 2 cut(s) 75, 653
GsaI CCCAGC 1 cut(s) 32
HaeIII GGCC 2 cut(s) 77, 269
Hin1II CATG 2 cut(s) 434, 509
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HinfI GANTC 2 cut(s) 434, 782
HphI GGTGA 2 cut(s) 334, 707
Hpy166II GTNNAC 2 cut(s) 200, 227
Hpy188III TCNNGA 3 cut(s) 197, 431, 714
Hpy8I GTNNAC 2 cut(s) 200, 227
HpyAV CCTTC 5 cut(s) 319, 525, 686, 713, 758
HpyCH4IV ACGT 4 cut(s) 166, 202, 246, 380
HpyCH4V TGCA 5 cut(s) 23, 299, 490, 528, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 4 cut(s) 166, 202, 246, 380
Hsp92II CATG 2 cut(s) 434, 509
Kzo9I GATC 4 cut(s) 148, 643, 676, 747
LmnI GCTCC 1 cut(s) 456
LpnPI CCDG 9 cut(s) 14, 100, 159, 323, 340, 350, 367, 697, 727
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 2 cut(s) 385, 581
MaeII ACGT 4 cut(s) 166, 202, 246, 380
MaeIII GTNAC 1 cut(s) 469
MalI GATC 4 cut(s) 150, 645, 678, 749
MbiI CCGCTC 1 cut(s) 320
MboI GATC 4 cut(s) 148, 643, 676, 747
MboII GAAGA 5 cut(s) 72, 75, 287, 503, 752
MflI RGATCY 2 cut(s) 643, 676
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 5 cut(s) 37, 308, 631, 664, 790
MlyI GAGTC 2 cut(s) 428, 791
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 3 cut(s) 180, 379, 723
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 4 cut(s) 45, 408, 593, 789
MspA1I CMGCKG 1 cut(s) 139
MspR9I CCNGG 2 cut(s) 338, 355
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 338, 355
MwoI GCNNNNNNNGC 4 cut(s) 25, 266, 326, 395
NdeII GATC 4 cut(s) 148, 643, 676, 747
NlaIII CATG 2 cut(s) 434, 509
PagI TCATGA 1 cut(s) 430
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 76, 654
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 2 cut(s) 428, 790
PpsI GAGTC 2 cut(s) 428, 790
Psp1406I AACGTT 3 cut(s) 202, 246, 380
Psp6I CCWGG 2 cut(s) 336, 353
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 2 cut(s) 336, 353
PspPI GGNCC 1 cut(s) 176
PsuI RGATCY 2 cut(s) 643, 676
PvuI CGATCG 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 2 cut(s) 344, 368
RsaNI GTAC 2 cut(s) 343, 367
SaqAI TTAA 4 cut(s) 45, 408, 593, 789
SatI GCNGC 2 cut(s) 75, 653
Sau3AI GATC 4 cut(s) 148, 643, 676, 747
Sau96I GGNCC 1 cut(s) 176
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 2 cut(s) 428, 791
ScrFI CCNGG 2 cut(s) 338, 355
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 2 cut(s) 385, 581
SfcI CTRYAG 1 cut(s) 685
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 98, 399
SmoI CTYRAG 2 cut(s) 98, 399
Sse9I AATT 5 cut(s) 37, 308, 631, 664, 790
SsiI CCGC 4 cut(s) 74, 184, 215, 320
StyD4I CCNGG 2 cut(s) 336, 353
StyI CCWWGG 1 cut(s) 179
TaiI ACGT 4 cut(s) 169, 205, 249, 383
TaqII GACCGA 1 cut(s) 555
TasI AATT 5 cut(s) 37, 308, 631, 664, 790
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 4 cut(s) 45, 408, 593, 789
Tru9I TTAA 4 cut(s) 45, 408, 593, 789
TscAI CASTG 1 cut(s) 758
TseI GCWGC 1 cut(s) 652
TspDTI ATGAA 3 cut(s) 141, 158, 419
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.