FvH4_4g35140

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001403
Physical Location & Seq
Reverse (-)
32556022 .. 32559953
3932 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35140.t1

Sequence Viewer

Length: 1029 bp
ATGTCAGGGTCTTCCAGGGGAAACGTCACCATCACTCTTGGGCGCGGCCAGGTTGTAAAGAGGTCTGTTACGGGTTCAGGTGCTGAGTCTCTTCCCACAGCCGGGGCGAAGCGGTCGGTTAGAGATAGGTTAGGGAGTAATGCAGATAGCTCTGTGTCGAATGGGAGTTCACGCAGCAGCAACAAACGGCAGCGTGGACATGGTGGTATGTCAAGTATGAACTCCAATGGTTTTGATGATGCGCGCATTGGTAAAGATGACCTCCGGTTTAAACTCATGCAAAAAAATGCATTTAGACGAGCTTTGAGTGATGATGATGATGATCAGAGGCATGGAGATCTTCGTGAGAAGCTGACAAAGGCAGTTCAGCGTCCTGTTCCTAGTGTTGAATCACGGGCACAGCAGCGCCTGCCTGAACCTCAAGAGAGTGGTTTCTTTAGTCGAGTTTCTTCTACGAGAAGTGCCGATGATTTGCCTCATATGGACTCGTTGAGAAATTCTTATTCTCCTTGGACACTGGACCATTTACGACGAAGATCCCCAGAGAGAGTTCAAGTCACTTATAGGGGTCTCTCCCCTCAGAGGAATGTAGAAGACTTGCAGAGAAGGCCATTAAATAGGACATATGATGATGTCAGATCTGTTCCATATATGAAAAAAGATGTTTTAGATGCTACAAGGCCTGCGACTACTGCACCTTTCATGTCTAATACTGCATTGCCTCCTGGACCTGCAAAGCATATGGCACCCGTCCCGCCCCATATGAACCAAGTTCCTCCGCTGAGTAGCACTGTACAGAAACCTCCATACGTGGGTGATGAACAGCAGACAGTAGATGGCTTGCTGCAACATCTCGGATTGGGAAAATATGCCATTATATTCAAGGCTGAGGAAATTGATATGACTGCATTGAAGCAGATGGGAGAAAATGACCTCAAAGAGCTGGGAATACCTATGGGACCTAGGAAAAAGCTCCTGCTTGCTCTCTTGCCTCATTCCAAGCGGCCACTGGCCACAAATTTACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

343

Amino Acids

37.88

Weight (kDa)

10.21

Isoelectric Point (pI)

65.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 275 - 329 2.3e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 276 - 328 2.6e-08 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 739
AccB1I GGYRCC 1 cut(s) 745
AccII CGCG 2 cut(s) 45, 244
AciI CCGC 5 cut(s) 45, 112, 755, 779, 1003
AclWI GGATC 1 cut(s) 531
AcoI YGGCCR 3 cut(s) 46, 1004, 1011
AcsI RAATTY 2 cut(s) 496, 1018
AfaI GTAC 1 cut(s) 795
AfiI CCNNNNNNNGG 4 cut(s) 101, 102, 582, 812
AgsI TTSAA 4 cut(s) 389, 554, 883, 913
AjnI CCWGG 3 cut(s) 14, 48, 724
AluBI AGCT 5 cut(s) 150, 302, 352, 943, 973
AluI AGCT 5 cut(s) 150, 302, 352, 943, 973
Alw26I GTCTC 2 cut(s) 93, 575
AlwI GGATC 1 cut(s) 531
AlwNI CAGNNNCTG 2 cut(s) 83, 409
AoxI GGCC 5 cut(s) 46, 608, 680, 1004, 1011
ApeKI GCWGC 5 cut(s) 174, 177, 190, 403, 844
ApoI RAATTY 2 cut(s) 496, 1018
AspA2I CCTAGG 1 cut(s) 962
AspLEI GCGC 4 cut(s) 45, 244, 246, 408
AspS9I GGNCC 3 cut(s) 520, 728, 959
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 19, 827
AvaII GGWCC 3 cut(s) 520, 728, 959
AvrII CCTAGG 1 cut(s) 962
BaeGI GKGCMC 1 cut(s) 400
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 745
BbsI GAAGAC 2 cut(s) 3, 600
BbvCI CCTCAGC 1 cut(s) 888
BbvI GCAGC 5 cut(s) 186, 189, 202, 415, 831
BccI CCATC 3 cut(s) 38, 830, 913
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 3 cut(s) 16, 50, 726
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 93, 575
BfaI CTAG 2 cut(s) 381, 963
BfoI RGCGCY 1 cut(s) 409
BfuAI ACCTGC 1 cut(s) 739
BglII AGATCT 2 cut(s) 337, 638
BisI GCNGC 7 cut(s) 46, 175, 178, 191, 404, 845, 1004
BlnI CCTAGG 1 cut(s) 962
BlsI GCNGC 7 cut(s) 47, 176, 179, 192, 405, 846, 1005
Bme1390I CCNGG 4 cut(s) 16, 50, 103, 726
Bme18I GGWCC 3 cut(s) 520, 728, 959
BmgT120I GGNCC 3 cut(s) 520, 728, 959
BmiI GGNNCC 2 cut(s) 747, 960
BmrFI CCNGG 4 cut(s) 16, 50, 103, 726
BmsI GCATC 2 cut(s) 229, 661
BpiI GAAGAC 2 cut(s) 3, 600
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 103
BsaAI YACGTR 1 cut(s) 811
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 4 cut(s) 15, 102, 509, 962
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 102, 582, 812
Bse1I ACTGG 2 cut(s) 522, 1014
Bse3DI GCAATG 1 cut(s) 716
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 3 cut(s) 16, 50, 726
BseDI CCNNGG 4 cut(s) 15, 102, 509, 962
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 4 cut(s) 101, 102, 582, 812
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 4 cut(s) 75, 593, 773, 879
BseNI ACTGG 2 cut(s) 522, 1014
BsePI GCGCGC 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 5 cut(s) 186, 189, 202, 415, 831
BseYI CCCAGC 1 cut(s) 943
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 45, 244
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 5 cut(s) 48, 610, 682, 1006, 1013
BshNI GGYRCC 1 cut(s) 745
BsiEI CGRYCG 1 cut(s) 116
BsiSI CCGG 2 cut(s) 102, 265
BslFI GGGAC 2 cut(s) 737, 972
BslI CCNNNNNNNGG 4 cut(s) 101, 102, 582, 812
BsmAI GTCTC 2 cut(s) 93, 575
BsmFI GGGAC 2 cut(s) 737, 972
BsnI GGCC 5 cut(s) 48, 610, 682, 1006, 1013
Bso31I GGTCTC 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 400
Bsp1407I TGTACA 1 cut(s) 793
Bsp143I GATC 4 cut(s) 322, 337, 536, 638
BspACI CCGC 5 cut(s) 45, 112, 755, 779, 1003
BspANI GGCC 5 cut(s) 48, 610, 682, 1006, 1013
BspCNI CTCAG 4 cut(s) 76, 592, 774, 880
BspFNI CGCG 2 cut(s) 45, 244
BspLI GGNNCC 2 cut(s) 747, 960
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 745
BspTNI GGTCTC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 716
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 2 cut(s) 522, 1014
BssECI CCNNGG 4 cut(s) 15, 102, 509, 962
BssHII GCGCGC 1 cut(s) 242
BssMI GATC 4 cut(s) 322, 337, 536, 638
BssT1I CCWWGG 2 cut(s) 509, 962
Bst2UI CCWGG 3 cut(s) 16, 50, 726
Bst4CI ACNGT 2 cut(s) 793, 832
Bst6I CTCTTC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 1 cut(s) 793
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 5 cut(s) 244, 410, 684, 842, 981
BstDEI CTNAG 4 cut(s) 84, 579, 782, 888
BstFNI CGCG 2 cut(s) 45, 244
BstH2I RGCGCY 1 cut(s) 409
BstHHI GCGC 4 cut(s) 45, 244, 246, 408
BstKTI GATC 4 cut(s) 325, 340, 539, 641
BstMAI GTCTC 2 cut(s) 93, 575
BstMBI GATC 4 cut(s) 322, 337, 536, 638
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 3 cut(s) 409, 607, 692
BstNI CCWGG 3 cut(s) 16, 50, 726
BstSCI CCNGG 4 cut(s) 14, 48, 101, 724
BstSLI GKGCMC 1 cut(s) 400
BstUI CGCG 2 cut(s) 45, 244
BstV1I GCAGC 5 cut(s) 186, 189, 202, 415, 831
BstV2I GAAGAC 2 cut(s) 3, 600
BstX2I RGATCY 3 cut(s) 337, 536, 638
BstYI RGATCY 3 cut(s) 337, 536, 638
BsuRI GGCC 5 cut(s) 48, 610, 682, 1006, 1013
BtsIMutI CAGTG 3 cut(s) 515, 789, 1007
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 5 cut(s) 244, 410, 684, 842, 981
CaiI CAGNNNCTG 2 cut(s) 83, 409
CfoI GCGC 4 cut(s) 45, 244, 246, 408
Cfr13I GGNCC 3 cut(s) 520, 728, 959
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 794
CviAII CATG 4 cut(s) 200, 277, 332, 703
CviQI GTAC 1 cut(s) 794
DdeI CTNAG 4 cut(s) 84, 579, 782, 888
DpnI GATC 4 cut(s) 324, 339, 538, 640
DpnII GATC 4 cut(s) 322, 337, 536, 638
DraI TTTAAA 1 cut(s) 271
EaeI YGGCCR 3 cut(s) 46, 1004, 1011
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 509, 962
Eco147I AGGCCT 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 3 cut(s) 520, 728, 959
EcoO109I RGGNCCY 1 cut(s) 959
EcoRII CCWGG 3 cut(s) 14, 48, 724
EcoT14I CCWWGG 2 cut(s) 509, 962
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 2 cut(s) 509, 962
FaeI CATG 4 cut(s) 203, 280, 335, 706
FaqI GGGAC 2 cut(s) 737, 972
FatI CATG 4 cut(s) 199, 276, 331, 702
FauI CCCGC 1 cut(s) 762
FauNDI CATATG 4 cut(s) 480, 625, 741, 762
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 7 cut(s) 46, 175, 178, 191, 404, 845, 1004
Fsp4HI GCNGC 7 cut(s) 46, 175, 178, 191, 404, 845, 1004
FspBI CTAG 2 cut(s) 381, 963
GlaI GCGC 4 cut(s) 44, 243, 245, 407
GluI GCNGC 7 cut(s) 46, 175, 178, 191, 404, 845, 1004
GsaI CCCAGC 1 cut(s) 947
HaeII RGCGCY 1 cut(s) 409
HaeIII GGCC 5 cut(s) 48, 610, 682, 1006, 1013
HapII CCGG 2 cut(s) 102, 265
HgaI GACGC 1 cut(s) 359
HhaI GCGC 4 cut(s) 45, 244, 246, 408
Hin1II CATG 4 cut(s) 203, 280, 335, 706
Hin6I GCGC 4 cut(s) 43, 242, 244, 406
HinP1I GCGC 4 cut(s) 43, 242, 244, 406
HinfI GANTC 3 cut(s) 86, 389, 485
HpaII CCGG 2 cut(s) 102, 265
HphI GGTGA 2 cut(s) 19, 827
Hpy166II GTNNAC 2 cut(s) 170, 197
Hpy188I TCNGA 4 cut(s) 327, 582, 638, 857
Hpy188III TCNNGA 2 cut(s) 344, 422
Hpy8I GTNNAC 2 cut(s) 170, 197
Hpy99I CGWCG 1 cut(s) 534
HpyAV CCTTC 1 cut(s) 600
HpyCH4III ACNGT 2 cut(s) 793, 832
HpyCH4IV ACGT 3 cut(s) 24, 810, 1024
HpyCH4V TGCA 9 cut(s) 143, 280, 290, 601, 695, 716, 734, 847, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 409, 607, 692
HpyF3I CTNAG 4 cut(s) 84, 579, 782, 888
HpySE526I ACGT 3 cut(s) 24, 810, 1024
Hsp92II CATG 4 cut(s) 203, 280, 335, 706
HspAI GCGC 4 cut(s) 43, 242, 244, 406
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 4 cut(s) 322, 337, 536, 638
LmnI GCTCC 1 cut(s) 978
Lsp1109I GCAGC 5 cut(s) 186, 189, 202, 415, 831
LweI GCATC 2 cut(s) 229, 661
MaeI CTAG 2 cut(s) 381, 963
MaeII ACGT 3 cut(s) 24, 810, 1024
MaeIII GTNAC 3 cut(s) 25, 67, 556
MalI GATC 4 cut(s) 324, 339, 538, 640
MboI GATC 4 cut(s) 322, 337, 536, 638
MboII GAAGA 6 cut(s) 3, 83, 332, 441, 546, 605
MflI RGATCY 3 cut(s) 337, 536, 638
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 3 cut(s) 496, 894, 1018
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 95, 479
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 292
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 2 cut(s) 270, 614
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 1 cut(s) 781
MspI CCGG 2 cut(s) 102, 265
MspR9I CCNGG 4 cut(s) 16, 50, 103, 726
MssI GTTTAAAC 1 cut(s) 271
MvaI CCWGG 3 cut(s) 16, 50, 726
MvnI CGCG 2 cut(s) 45, 244
MwoI GCNNNNNNNGC 3 cut(s) 409, 607, 692
NciI CCSGG 1 cut(s) 103
NdeI CATATG 4 cut(s) 480, 625, 741, 762
NdeII GATC 4 cut(s) 322, 337, 536, 638
NlaIII CATG 4 cut(s) 203, 280, 335, 706
NlaIV GGNNCC 2 cut(s) 747, 960
NmuCI GTSAC 2 cut(s) 25, 556
NsiI ATGCAT 1 cut(s) 292
PauI GCGCGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 682
PfeI GAWTC 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 7 cut(s) 47, 176, 179, 192, 405, 846, 1005
PleI GAGTC 2 cut(s) 94, 479
PmeI GTTTAAAC 1 cut(s) 271
PpsI GAGTC 2 cut(s) 94, 479
Ppu21I YACGTR 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 3 cut(s) 14, 48, 724
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 3 cut(s) 14, 48, 724
PspN4I GGNNCC 2 cut(s) 747, 960
PspPI GGNCC 3 cut(s) 520, 728, 959
PspPPI RGGWCCY 1 cut(s) 959
PstNI CAGNNNCTG 2 cut(s) 83, 409
PsuI RGATCY 3 cut(s) 337, 536, 638
PteI GCGCGC 1 cut(s) 242
RsaI GTAC 1 cut(s) 795
RsaNI GTAC 1 cut(s) 794
SaqAI TTAA 2 cut(s) 270, 614
SatI GCNGC 7 cut(s) 46, 175, 178, 191, 404, 845, 1004
Sau3AI GATC 4 cut(s) 322, 337, 536, 638
Sau96I GGNCC 3 cut(s) 520, 728, 959
SchI GAGTC 2 cut(s) 95, 479
ScrFI CCNGG 4 cut(s) 16, 50, 103, 726
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 2 cut(s) 229, 661
SinI GGWCC 3 cut(s) 520, 728, 959
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 3 cut(s) 496, 894, 1018
SseBI AGGCCT 1 cut(s) 682
SsiI CCGC 5 cut(s) 45, 112, 755, 779, 1003
SspMI CTAG 2 cut(s) 381, 963
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 4 cut(s) 14, 48, 101, 724
StyI CCWWGG 2 cut(s) 509, 962
TaaI ACNGT 2 cut(s) 793, 832
TaiI ACGT 3 cut(s) 27, 813, 1027
TaqI TCGA 2 cut(s) 158, 442
TasI AATT 3 cut(s) 496, 894, 1018
TatI WGTACW 1 cut(s) 793
TauI GCSGC 2 cut(s) 48, 1006
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 2 cut(s) 270, 614
Tru9I TTAA 2 cut(s) 270, 614
TscAI CASTG 3 cut(s) 522, 796, 1014
TseFI GTSAC 2 cut(s) 25, 556
TseI GCWGC 5 cut(s) 174, 177, 190, 403, 844
Tsp45I GTSAC 2 cut(s) 25, 556
TspDTI ATGAA 5 cut(s) 233, 668, 691, 779, 834
TspRI CASTG 3 cut(s) 522, 796, 1014
VpaK11BI GGWCC 3 cut(s) 520, 728, 959
XapI RAATTY 2 cut(s) 496, 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmaJI CCTAGG 1 cut(s) 962
XspI CTAG 2 cut(s) 381, 963
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.