Rw4G035670

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001403
Physical Location & Seq
Reverse (-)
62503097 .. 62507313
4217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035670.1

Sequence Viewer

Length: 1062 bp
ATGTCAGGGTCTTCCAGGGGCCAAGTCACCATTACGCTTGGGCGTGGCCAGGTAGTGAAGAGGGCTGTTTCGGGTTCGGGTGCTGAGTCTCTGCCCACGGCCGGGACGAAACGGTCGGTTAGAGATAGGTTAGGGAGTAATGCTGATAGCTCTGTGTCGAATGGAAGTGTAGGCAACAACAACAAACGCCAGCGAGGACAGAGTGGTATGTCAAGTTTGAACTCCAATGGTTGTGATGATCCATGCATTGGTAAAGACGACCTCCGGTTTAAACTCATGCAAAAAAATGCATGTAGACGGGCTCGGAGTGATGATGATGATCAGAGGCATGGAGATCTTCGTGAGAAGCTGTCAAAGGCAGTTCAGCGTCCTGTTCCTAGCGTTGAATCACGGTCACGGCAGCGCCTGCCTGAACCACAAGAGAGTGGTTTTTTCGGTCGAGTTCCTTCTACGAGAAGTGCAGATGTTTTGCCTCGAGGTCCTGCTACAAGAAGTGCAGATGATTTGCCTCATATGGACTCGTTGAGAAATTCTTATTCTCCTTGGACACTGGACCATTTACGACGAAGATCCCCAGAAAGAGTTCAAGTCACTTATAGGGGTCTCTCCCCTCAGAGGAACGTAGAAGACTTGCAGAGAAGGCCATTAAACAGGACATATGATGATGGCAGATCTGTTCCATACATGAACAAAGATGTTCTAGACGCTACAAGGCCTGCGAGTACTGCACCTTTCATGTCTAATACTGCACTGCCTCCTGGACCTGCAAAGCATATGGCAGCCGCCCCTCCCCATATGAACCAAGTTCCTCCACTGAGTAGCACTGTACAGAAACCTCCATATGTGGGTGATGAACAACAGACAGTAGATGGCTTGCTGCAACACCTCGGATTGGGAAAATATGCCATTATATTCAAGGCTGAGGAAATTGATATGACTGCATTGAAGCAGATGGGAGAAAATGACCTCAAAGAGCTGGGAATACCTATGGGACCTAGGAAAAAGCTTCTGCTTGCTCTCTTGCCTCGTTCCAAGCGGCCACTGCCCACAAATATACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

353

Amino Acids

38.83

Weight (kDa)

10.19

Isoelectric Point (pI)

68.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 286 - 340 2.4e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 287 - 339 2.8e-08 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 112
AbsI CCTCGAGG 1 cut(s) 474
Acc36I ACCTGC 1 cut(s) 772
AccB7I CCANNNNNTGG 1 cut(s) 248
AccI GTMKAC 1 cut(s) 295
AciI CCGC 2 cut(s) 783, 1036
AclWI GGATC 2 cut(s) 233, 564
AcoI YGGCCR 3 cut(s) 46, 99, 1037
AcsI RAATTY 1 cut(s) 529
AfaI GTAC 2 cut(s) 724, 828
AfiI CCNNNNNNNGG 6 cut(s) 101, 102, 248, 615, 845, 892
AgsI TTSAA 5 cut(s) 220, 386, 587, 916, 946
AjnI CCWGG 3 cut(s) 14, 48, 757
AluBI AGCT 4 cut(s) 150, 349, 976, 1006
AluI AGCT 4 cut(s) 150, 349, 976, 1006
Alw26I GTCTC 2 cut(s) 93, 608
AlwI GGATC 2 cut(s) 233, 564
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 6 cut(s) 19, 46, 99, 641, 713, 1037
ApeKI GCWGC 3 cut(s) 400, 779, 877
ApoI RAATTY 1 cut(s) 529
Asp700I GAANNNNTTC 1 cut(s) 582
AspA2I CCTAGG 1 cut(s) 995
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 5 cut(s) 19, 479, 553, 761, 992
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 19, 860
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 4 cut(s) 479, 553, 761, 992
AvrII CCTAGG 1 cut(s) 995
BalI TGGCCA 1 cut(s) 48
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 2 cut(s) 3, 633
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 3 cut(s) 412, 791, 864
BccI CCATC 3 cut(s) 659, 863, 946
BceAI ACGGC 2 cut(s) 114, 413
BciT130I CCWGG 3 cut(s) 16, 50, 759
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 93, 608
BfaI CTAG 3 cut(s) 378, 701, 996
BfoI RGCGCY 1 cut(s) 406
BfuAI ACCTGC 1 cut(s) 772
BglII AGATCT 2 cut(s) 334, 671
BisI GCNGC 5 cut(s) 401, 780, 783, 878, 1037
BlnI CCTAGG 1 cut(s) 995
BlsI GCNGC 5 cut(s) 402, 781, 784, 879, 1038
BmcAI AGTACT 1 cut(s) 724
Bme1390I CCNGG 4 cut(s) 16, 50, 103, 759
Bme18I GGWCC 4 cut(s) 479, 553, 761, 992
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 5 cut(s) 19, 479, 553, 761, 992
BmiI GGNNCC 2 cut(s) 20, 993
BmrFI CCNGG 4 cut(s) 16, 50, 103, 759
BpiI GAAGAC 2 cut(s) 3, 633
Bpu10I CCTNAGC 1 cut(s) 921
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 5 cut(s) 15, 96, 542, 886, 995
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 102, 248, 615, 845, 892
Bse1I ACTGG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 3 cut(s) 16, 50, 759
BseDI CCNNGG 5 cut(s) 15, 96, 542, 886, 995
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 6 cut(s) 101, 102, 248, 615, 845, 892
BseMII CTCAG 4 cut(s) 75, 626, 806, 912
BseNI ACTGG 1 cut(s) 555
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 3 cut(s) 412, 791, 864
BseYI CCCAGC 1 cut(s) 976
BsgI GTGCAG 4 cut(s) 480, 516, 711, 732
Bsh1285I CGRYCG 3 cut(s) 102, 116, 439
BshFI GGCC 6 cut(s) 21, 48, 101, 643, 715, 1039
BsiEI CGRYCG 3 cut(s) 102, 116, 439
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 2 cut(s) 102, 265
BslFI GGGAC 2 cut(s) 118, 1005
BslI CCNNNNNNNGG 6 cut(s) 101, 102, 248, 615, 845, 892
BsmAI GTCTC 2 cut(s) 93, 608
BsmFI GGGAC 2 cut(s) 118, 1005
BsnI GGCC 6 cut(s) 21, 48, 101, 643, 715, 1039
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 304
Bsp1407I TGTACA 1 cut(s) 826
Bsp143I GATC 5 cut(s) 238, 319, 334, 569, 671
BspACI CCGC 2 cut(s) 783, 1036
BspANI GGCC 6 cut(s) 21, 48, 101, 643, 715, 1039
BspCNI CTCAG 4 cut(s) 76, 625, 807, 913
BspLI GGNNCC 2 cut(s) 20, 993
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 2 cut(s) 233, 564
BspTNI GGTCTC 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 826
BsrI ACTGG 1 cut(s) 555
BssECI CCNNGG 5 cut(s) 15, 96, 542, 886, 995
BssMI GATC 5 cut(s) 238, 319, 334, 569, 671
BssT1I CCWWGG 2 cut(s) 542, 995
Bst2UI CCWGG 3 cut(s) 16, 50, 759
Bst4CI ACNGT 4 cut(s) 114, 393, 826, 865
Bst6I CTCTTC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 826
BstC8I GCNNGC 5 cut(s) 191, 407, 717, 875, 1014
BstDEI CTNAG 4 cut(s) 84, 612, 815, 921
BstDSI CCRYGG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 406
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 5 cut(s) 241, 322, 337, 572, 674
BstMAI GTCTC 2 cut(s) 93, 608
BstMBI GATC 5 cut(s) 238, 319, 334, 569, 671
BstMCI CGRYCG 3 cut(s) 102, 116, 439
BstMWI GCNNNNNNNGC 4 cut(s) 406, 640, 725, 1042
BstNI CCWGG 3 cut(s) 16, 50, 759
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 4 cut(s) 14, 48, 101, 757
BstV1I GCAGC 3 cut(s) 412, 791, 864
BstV2I GAAGAC 2 cut(s) 3, 633
BstX2I RGATCY 3 cut(s) 334, 569, 671
BstYI RGATCY 3 cut(s) 334, 569, 671
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 6 cut(s) 21, 48, 101, 643, 715, 1039
BtgI CCRYGG 1 cut(s) 96
BtsI GCAGTG 2 cut(s) 749, 1040
BtsIMutI CAGTG 5 cut(s) 548, 749, 812, 822, 1040
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 5 cut(s) 191, 407, 717, 875, 1014
CaiI CAGNNNCTG 1 cut(s) 406
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 5 cut(s) 19, 479, 553, 761, 992
CseI GACGC 2 cut(s) 356, 713
Csp6I GTAC 2 cut(s) 723, 827
CviAII CATG 6 cut(s) 243, 277, 291, 329, 685, 736
CviQI GTAC 2 cut(s) 723, 827
DdeI CTNAG 4 cut(s) 84, 612, 815, 921
DpnI GATC 5 cut(s) 240, 321, 336, 571, 673
DpnII GATC 5 cut(s) 238, 319, 334, 569, 671
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 3 cut(s) 46, 99, 1037
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 542, 995
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 4 cut(s) 479, 553, 761, 992
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 474
EcoO109I RGGNCCY 2 cut(s) 479, 992
EcoRII CCWGG 3 cut(s) 14, 48, 757
EcoT14I CCWWGG 2 cut(s) 542, 995
EcoT22I ATGCAT 2 cut(s) 248, 292
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 542, 995
FaeI CATG 6 cut(s) 246, 280, 294, 332, 688, 739
FaqI GGGAC 2 cut(s) 118, 1005
FatI CATG 6 cut(s) 242, 276, 290, 328, 684, 735
FauNDI CATATG 5 cut(s) 513, 658, 774, 795, 841
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 401, 780, 783, 878, 1037
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 5 cut(s) 401, 780, 783, 878, 1037
FspBI CTAG 3 cut(s) 378, 701, 996
GlaI GCGC 1 cut(s) 404
GluI GCNGC 5 cut(s) 401, 780, 783, 878, 1037
GsaI CCCAGC 1 cut(s) 980
HaeII RGCGCY 1 cut(s) 406
HaeIII GGCC 6 cut(s) 21, 48, 101, 643, 715, 1039
HapII CCGG 2 cut(s) 102, 265
HgaI GACGC 2 cut(s) 356, 713
HhaI GCGC 1 cut(s) 405
Hin1II CATG 6 cut(s) 246, 280, 294, 332, 688, 739
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 1004
HinfI GANTC 3 cut(s) 86, 386, 518
HpaII CCGG 2 cut(s) 102, 265
HphI GGTGA 2 cut(s) 19, 860
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 4 cut(s) 306, 324, 615, 890
Hpy188III TCNNGA 2 cut(s) 341, 701
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 1 cut(s) 567
HpyAV CCTTC 2 cut(s) 456, 633
HpyCH4III ACNGT 4 cut(s) 114, 393, 826, 865
HpyCH4IV ACGT 2 cut(s) 621, 1057
HpyF10VI GCNNNNNNNGC 4 cut(s) 406, 640, 725, 1042
HpyF3I CTNAG 4 cut(s) 84, 612, 815, 921
HpySE526I ACGT 2 cut(s) 621, 1057
Hsp92II CATG 6 cut(s) 246, 280, 294, 332, 688, 739
HspAI GCGC 1 cut(s) 403
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 238, 319, 334, 569, 671
Lsp1109I GCAGC 3 cut(s) 412, 791, 864
MaeI CTAG 3 cut(s) 378, 701, 996
MaeII ACGT 2 cut(s) 621, 1057
MaeIII GTNAC 3 cut(s) 25, 393, 589
MalI GATC 5 cut(s) 240, 321, 336, 571, 673
MboI GATC 5 cut(s) 238, 319, 334, 569, 671
MboII GAAGA 5 cut(s) 3, 70, 329, 579, 638
MflI RGATCY 3 cut(s) 334, 569, 671
MhlI GDGCHC 1 cut(s) 304
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 2 cut(s) 529, 927
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 95, 512
Mox20I TGGCCA 1 cut(s) 48
Mph1103I ATGCAT 2 cut(s) 248, 292
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 270, 647
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 102, 265
MspR9I CCNGG 4 cut(s) 16, 50, 103, 759
MssI GTTTAAAC 1 cut(s) 271
MvaI CCWGG 3 cut(s) 16, 50, 759
MwoI GCNNNNNNNGC 4 cut(s) 406, 640, 725, 1042
NciI CCSGG 1 cut(s) 103
NdeI CATATG 5 cut(s) 513, 658, 774, 795, 841
NdeII GATC 5 cut(s) 238, 319, 334, 569, 671
NlaIII CATG 6 cut(s) 246, 280, 294, 332, 688, 739
NlaIV GGNNCC 2 cut(s) 20, 993
NmuCI GTSAC 3 cut(s) 25, 393, 589
NsiI ATGCAT 2 cut(s) 248, 292
NspI RCATGY 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 474
PceI AGGCCT 1 cut(s) 715
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 5 cut(s) 402, 781, 784, 879, 1038
PleI GAGTC 2 cut(s) 94, 512
PmeI GTTTAAAC 1 cut(s) 271
PpsI GAGTC 2 cut(s) 94, 512
PpuMI RGGWCCY 2 cut(s) 479, 992
Psp5II RGGWCCY 2 cut(s) 479, 992
Psp6I CCWGG 3 cut(s) 14, 48, 757
PspFI CCCAGC 1 cut(s) 976
PspGI CCWGG 3 cut(s) 14, 48, 757
PspN4I GGNNCC 2 cut(s) 20, 993
PspPI GGNCC 5 cut(s) 19, 479, 553, 761, 992
PspPPI RGGWCCY 2 cut(s) 479, 992
PspXI VCTCGAGB 1 cut(s) 474
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 3 cut(s) 334, 569, 671
RsaI GTAC 2 cut(s) 724, 828
RsaNI GTAC 2 cut(s) 723, 827
SaqAI TTAA 2 cut(s) 270, 647
SatI GCNGC 5 cut(s) 401, 780, 783, 878, 1037
Sau3AI GATC 5 cut(s) 238, 319, 334, 569, 671
Sau96I GGNCC 5 cut(s) 19, 479, 553, 761, 992
ScaI AGTACT 1 cut(s) 724
SchI GAGTC 2 cut(s) 95, 512
ScrFI CCNGG 4 cut(s) 16, 50, 103, 759
SduI GDGCHC 1 cut(s) 304
Sfr274I CTCGAG 1 cut(s) 474
SinI GGWCC 4 cut(s) 479, 553, 761, 992
SlaI CTCGAG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 2 cut(s) 529, 927
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 2 cut(s) 783, 1036
SspMI CTAG 3 cut(s) 378, 701, 996
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 4 cut(s) 14, 48, 101, 757
StyI CCWWGG 2 cut(s) 542, 995
TaaI ACNGT 4 cut(s) 114, 393, 826, 865
TaiI ACGT 2 cut(s) 624, 1060
TaqI TCGA 3 cut(s) 158, 439, 475
TaqII GACCGA 1 cut(s) 425
TasI AATT 2 cut(s) 529, 927
TatI WGTACW 2 cut(s) 722, 826
TauI GCSGC 2 cut(s) 785, 1039
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 270, 647
Tru9I TTAA 2 cut(s) 270, 647
TscAI CASTG 5 cut(s) 555, 756, 819, 829, 1047
TseFI GTSAC 3 cut(s) 25, 393, 589
TseI GCWGC 3 cut(s) 400, 779, 877
Tsp45I GTSAC 3 cut(s) 25, 393, 589
TspDTI ATGAA 4 cut(s) 701, 724, 812, 867
TspRI CASTG 5 cut(s) 555, 756, 819, 829, 1047
Van91I CCANNNNNTGG 1 cut(s) 248
VpaK11BI GGWCC 4 cut(s) 479, 553, 761, 992
XapI RAATTY 1 cut(s) 529
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 1 cut(s) 294
XhoI CTCGAG 1 cut(s) 474
XmaJI CCTAGG 1 cut(s) 995
XmiI GTMKAC 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 3 cut(s) 378, 701, 996
ZrmI AGTACT 1 cut(s) 724
Zsp2I ATGCAT 2 cut(s) 248, 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.