FvH4_6g19601

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001403
Physical Location & Seq
Reverse (-)
13108508 .. 13109576
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19601.t1

Sequence Viewer

Length: 480 bp
ATGTCACAGCATGAGTTGGAAACAAATGGGAGTAGCTTCCTGAGAGAAATGCCTCCCAGGGATAGTGGAGCTGATTTGTACCATGTAAATTCACAGAGGAACAAAGAAGAGATGGATTCCAAGCTAGATCCCCTGATAGCTTTCAGAACACTTCTTACGGGCCATCACCACAAAGAAACTTTGGTGAGCTGCAGTCAGCATCATCATTCAGGCCAGTTGACGCTCAAAGAGCTGGCCACTTGTGCCAACAAATTTACAGCATGCAAAATTATATGGGGGAAGAACCTAAGTGTTGCTAGTTTATTGCATAGACTTGGTTTGGGAAAATATGCTATAATCTTCCAGGCTGAAGAAGTAGACATGAGCGCTTTGAAGCAGATGGGCGATAAGGACCTTAAAGAGCTGGGTATACCAATGGGGCCAAGGAAAAAGATTCTCCTTGCACTTCTCGCCTGTGGTAACCGACCACCGCCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

160

Amino Acids

17.69

Weight (kDa)

8.7

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 97 - 149 2.1e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 97 - 148 8.3e-09 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 357, 409
AciI CCGC 1 cut(s) 470
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 2 cut(s) 88, 251
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 80
AfeI AGCGCT 1 cut(s) 367
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 2 cut(s) 56, 342
AluBI AGCT 7 cut(s) 36, 71, 124, 140, 189, 232, 403
AluI AGCT 7 cut(s) 36, 71, 124, 140, 189, 232, 403
AlwI GGATC 1 cut(s) 122
Aor51HI AGCGCT 1 cut(s) 367
AoxI GGCC 4 cut(s) 160, 211, 234, 419
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 88, 251
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 3 cut(s) 160, 391, 419
AsuHPI GGTGA 2 cut(s) 158, 196
AvaII GGWCC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 236
BbvI GCAGC 1 cut(s) 176
BccI CCATC 3 cut(s) 106, 171, 373
BciT130I CCWGG 2 cut(s) 58, 344
BfaI CTAG 2 cut(s) 125, 297
BfmI CTRYAG 1 cut(s) 190
BfoI RGCGCY 1 cut(s) 369
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 58, 344
Bme18I GGWCC 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 160, 391, 419
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 2 cut(s) 58, 344
BmsI GCATC 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 56, 57, 422
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 58, 344
BseDI CCNNGG 3 cut(s) 56, 57, 422
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 176
BseYI CCCAGC 1 cut(s) 403
BshFI GGCC 4 cut(s) 162, 213, 236, 421
BsnI GGCC 4 cut(s) 162, 213, 236, 421
Bsp143I GATC 1 cut(s) 127
BspACI CCGC 1 cut(s) 470
BspANI GGCC 4 cut(s) 162, 213, 236, 421
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 420
BspMAI CTGCAG 1 cut(s) 194
BspPI GGATC 1 cut(s) 122
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 56, 57, 422
BssMI GATC 1 cut(s) 127
BssNAI GTATAC 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 422
Bst1107I GTATAC 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 58, 344
Bst4CI ACNGT 1 cut(s) 477
Bst6I CTCTTC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 234, 262
BstDEI CTNAG 2 cut(s) 41, 287
BstEII GGTNACC 1 cut(s) 458
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 1 cut(s) 130
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 3 cut(s) 229, 242, 449
BstNI CCWGG 2 cut(s) 58, 344
BstNSI RCATGY 1 cut(s) 264
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 56, 342
BstSFI CTRYAG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 176
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZ17I GTATAC 1 cut(s) 410
BsuRI GGCC 4 cut(s) 162, 213, 236, 421
Cac8I GCNNGC 2 cut(s) 234, 262
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 3 cut(s) 160, 391, 419
CseI GACGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 79
CviAII CATG 4 cut(s) 11, 83, 261, 361
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 41, 287
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 391
Eco47III AGCGCT 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 369
Eco91I GGTNACC 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 391
EcoO65I GGTNACC 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 56, 342
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 4 cut(s) 14, 86, 264, 364
FatI CATG 4 cut(s) 10, 82, 260, 360
FblI GTMKAC 2 cut(s) 357, 409
Fnu4HI GCNGC 1 cut(s) 190
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 2 cut(s) 125, 297
GlaI GCGC 1 cut(s) 367
GluI GCNGC 1 cut(s) 190
GsaI CCCAGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 4 cut(s) 162, 213, 236, 421
HgaI GACGC 1 cut(s) 229
HhaI GCGC 1 cut(s) 368
Hin1II CATG 4 cut(s) 14, 86, 264, 364
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 2 cut(s) 116, 433
HphI GGTGA 2 cut(s) 158, 196
Hpy166II GTNNAC 3 cut(s) 219, 358, 410
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 3 cut(s) 219, 358, 410
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4V TGCA 4 cut(s) 192, 264, 307, 443
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 242, 449
HpyF3I CTNAG 2 cut(s) 41, 287
Hsp92II CATG 4 cut(s) 14, 86, 264, 364
HspAI GCGC 1 cut(s) 366
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 1 cut(s) 208
MaeI CTAG 2 cut(s) 125, 297
MaeIII GTNAC 2 cut(s) 3, 458
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 4 cut(s) 119, 292, 331, 362
MflI RGATCY 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 3 cut(s) 88, 251, 267
MluNI TGGCCA 1 cut(s) 236
MnlI CCTC 2 cut(s) 63, 90
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
MseI TTAA 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 236
MspR9I CCNGG 2 cut(s) 58, 344
MvaI CCWGG 2 cut(s) 58, 344
MwoI GCNNNNNNNGC 3 cut(s) 229, 242, 449
NdeII GATC 1 cut(s) 127
NlaIII CATG 4 cut(s) 14, 86, 264, 364
NlaIV GGNNCC 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 264
PaeI GCATGC 1 cut(s) 264
PasI CCCWGGG 1 cut(s) 57
PfeI GAWTC 2 cut(s) 116, 433
PkrI GCNGC 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 56, 342
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 2 cut(s) 56, 342
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 3 cut(s) 160, 391, 419
PspPPI RGGWCCY 1 cut(s) 391
PsrI GAACNNNNNNTAC 2 cut(s) 139, 171
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 3 cut(s) 160, 391, 419
ScrFI CCNGG 2 cut(s) 58, 344
SetI ASST 9 cut(s) 38, 73, 126, 142, 191, 234, 288, 396, 405
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 190
SinI GGWCC 1 cut(s) 391
SphI GCATGC 1 cut(s) 264
Sse9I AATT 3 cut(s) 88, 251, 267
SsiI CCGC 1 cut(s) 470
SspMI CTAG 2 cut(s) 125, 297
StyD4I CCNGG 2 cut(s) 56, 342
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 1 cut(s) 477
TasI AATT 3 cut(s) 88, 251, 267
TfiI GAWTC 2 cut(s) 116, 433
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 1 cut(s) 3
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 2 cut(s) 88, 251
XceI RCATGY 1 cut(s) 264
XmiI GTMKAC 2 cut(s) 357, 409
XspI CTAG 2 cut(s) 125, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.