Rroxscaffold_2G00081410

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001403
Physical Location & Seq
Reverse (-)
4457563 .. 4461399
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081410.1

Sequence Viewer

Length: 954 bp
ATGTCGAAGCCCAGGGTCACCATCACCCTCGGTCGCTCTGGTCAGGTTGTGGAGAGGGGAGGGACATCAGACTCTGTAAACATGCGTGGCGAAAGAGGTATGGTTTCGGGAGGTAAACGATCAATGGGAGACAGGCTTGGGAGTAATGCTCATGGAATGCGGCAACGAGGAGATGGAATGAAATGGAGAGGGGGTGACAATACATTACCTGATTCACGAATCAGTCGAAATGACCTCAGATTGAAACTGTTGCGCAAAAGATCATTTAAGCAAAATGGGGGAGTTGTTGCAGAACGCAAGCAGATGGTCCCGCGCCCAAAGCTGTCAAAACCTGCTCACTCTGCAGTGAGGTATCAGATGCCACAGCACAAGTCAGAAACAAATGAGAGTAGCTTCCTGAGGCAAATTCCTCCCAGGGAAACTGCAGCTGATTTGTATCATGTAAATCCACAGAGGAACTTCAACTCTTCTCAAAGTAGAGAAGGATTCCGAGCTAGATCCCCTGATAACTTTGTGAACACTTCTTATGGGCCATCACCTCAAAGAAACTTTGGTGAGCTGCAGTCAGCATCATCCTTCAGGGCAGCTGACGCTCCTAGAGCTGGCCAGTTCCTGAGAAATGGTATGGTTGGTTCGTCTCAGCCAACAGATTCCTTCCTTTTTAGAACTAAATCTAACCCTCAAACTGCCAAGCCAGTTGGACAGCTTGTGCCAACAAATTACAGCATGCAAAAAGTCCCATATATGGGGGAGGAACCTCTAAGTGTTGCTAGTTTATTGCATAGACTTGGTTTGGGCAAATATGGTATTATTTTCCAGGCTGAAGAAGTAGACATGGGTGCTCTGAAGCAGATGGGGGATAAGGACCTTAAAGAGCTGGGTATACCAATGGGGCCAAGGAAAAAGATTCTTCTTGCGCTTTTGGCCCGTGGTAAACGACCACCGCCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

317

Amino Acids

35.06

Weight (kDa)

10.94

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 254 - 307 5.1e-12 SAM domain (Sterile alpha motif)
SAM_2 PF07647 255 - 306 4.8e-06 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 340
AccI GTMKAC 2 cut(s) 831, 883
AccII CGCG 1 cut(s) 313
AciI CCGC 3 cut(s) 160, 311, 944
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 3 cut(s) 562, 843, 866
AfiI CCNNNNNNNGG 3 cut(s) 602, 745, 746
AgsI TTSAA 2 cut(s) 244, 463
AjnI CCWGG 3 cut(s) 11, 413, 816
AluBI AGCT 9 cut(s) 322, 393, 428, 494, 559, 587, 602, 706, 877
AluI AGCT 9 cut(s) 322, 393, 428, 494, 559, 587, 602, 706, 877
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 2 cut(s) 123, 642
AlwI GGATC 1 cut(s) 492
AlwNI CAGNNNCTG 2 cut(s) 74, 613
AoxI GGCC 4 cut(s) 530, 604, 893, 924
ApeKI GCWGC 3 cut(s) 425, 559, 584
ApoI RAATTY 1 cut(s) 405
AspLEI GCGC 3 cut(s) 255, 315, 919
AspS9I GGNCC 5 cut(s) 307, 530, 865, 893, 925
AsuHPI GGTGA 5 cut(s) 10, 16, 206, 528, 566
AvaII GGWCC 2 cut(s) 307, 865
AxyI CCTNAGG 1 cut(s) 398
BalI TGGCCA 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 3 cut(s) 437, 546, 596
BccI CCATC 5 cut(s) 29, 167, 298, 541, 847
BciT130I CCWGG 3 cut(s) 13, 415, 818
BcoDI GTCTC 2 cut(s) 123, 642
BfaI CTAG 3 cut(s) 495, 597, 771
BfmI CTRYAG 3 cut(s) 342, 423, 560
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 4 cut(s) 161, 426, 560, 585
BlsI GCNGC 4 cut(s) 162, 427, 561, 586
Bme1390I CCNGG 3 cut(s) 13, 415, 818
Bme18I GGWCC 2 cut(s) 307, 865
BmgT120I GGNCC 5 cut(s) 307, 530, 865, 893, 925
BmiI GGNNCC 3 cut(s) 309, 756, 894
BmrFI CCNGG 3 cut(s) 13, 415, 818
BmsI GCATC 2 cut(s) 348, 578
BplI GAGNNNNNCTC 2 cut(s) 133, 165
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 7 cut(s) 11, 12, 28, 413, 414, 896, 928
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 3 cut(s) 602, 745, 746
Bse1I ACTGG 2 cut(s) 607, 695
Bse21I CCTNAGG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 13, 415, 818
BseDI CCNNGG 7 cut(s) 11, 12, 28, 413, 414, 896, 928
BseGI GGATG 1 cut(s) 572
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 3 cut(s) 602, 745, 746
BseMII CTCAG 4 cut(s) 250, 389, 605, 653
BseNI ACTGG 2 cut(s) 607, 695
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 3 cut(s) 437, 546, 596
BseYI CCCAGC 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 4 cut(s) 532, 606, 895, 926
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 844
BslFI GGGAC 3 cut(s) 76, 293, 722
BslI CCNNNNNNNGG 3 cut(s) 602, 745, 746
BsmAI GTCTC 2 cut(s) 123, 642
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 3 cut(s) 76, 293, 722
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 4 cut(s) 532, 606, 895, 926
Bsp1286I GDGCHC 1 cut(s) 844
Bsp143I GATC 3 cut(s) 119, 260, 497
BspACI CCGC 3 cut(s) 160, 311, 944
BspANI GGCC 4 cut(s) 532, 606, 895, 926
BspCNI CTCAG 4 cut(s) 249, 390, 606, 652
BspFNI CGCG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 309, 756, 894
BspMAI CTGCAG 3 cut(s) 346, 427, 564
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 492
BsrI ACTGG 2 cut(s) 607, 695
BssECI CCNNGG 7 cut(s) 11, 12, 28, 413, 414, 896, 928
BssMI GATC 3 cut(s) 119, 260, 497
BssNAI GTATAC 1 cut(s) 884
BssT1I CCWWGG 1 cut(s) 896
Bst1107I GTATAC 1 cut(s) 884
Bst2UI CCWGG 3 cut(s) 13, 415, 818
Bst4CI ACNGT 2 cut(s) 249, 951
Bst6I CTCTTC 1 cut(s) 472
BstC8I GCNNGC 3 cut(s) 299, 604, 728
BstDEI CTNAG 5 cut(s) 236, 398, 614, 639, 761
BstDSI CCRYGG 1 cut(s) 928
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 1 cut(s) 572
BstFNI CGCG 1 cut(s) 313
BstHHI GCGC 3 cut(s) 255, 315, 919
BstKTI GATC 3 cut(s) 122, 263, 500
BstMAI GTCTC 2 cut(s) 123, 642
BstMBI GATC 3 cut(s) 119, 260, 497
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 319, 341, 590, 599, 923
BstNI CCWGG 3 cut(s) 13, 415, 818
BstNSI RCATGY 2 cut(s) 85, 730
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 3 cut(s) 11, 413, 816
BstSFI CTRYAG 3 cut(s) 342, 423, 560
BstUI CGCG 1 cut(s) 313
BstV1I GCAGC 3 cut(s) 437, 546, 596
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BstZ17I GTATAC 1 cut(s) 884
Bsu36I CCTNAGG 1 cut(s) 398
BsuRI GGCC 4 cut(s) 532, 606, 895, 926
BtgI CCRYGG 1 cut(s) 928
BtsCI GGATG 1 cut(s) 572
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 351
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 3 cut(s) 299, 604, 728
CaiI CAGNNNCTG 2 cut(s) 74, 613
CfoI GCGC 3 cut(s) 255, 315, 919
Cfr13I GGNCC 5 cut(s) 307, 530, 865, 893, 925
CseI GACGC 1 cut(s) 599
CviAII CATG 5 cut(s) 82, 152, 440, 727, 835
DdeI CTNAG 5 cut(s) 236, 398, 614, 639, 761
DpnI GATC 3 cut(s) 121, 262, 499
DpnII GATC 3 cut(s) 119, 260, 497
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 896
Eco47I GGWCC 2 cut(s) 307, 865
Eco57I CTGAAG 3 cut(s) 562, 843, 866
Eco81I CCTNAGG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 865
EcoO65I GGTNACC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 11, 413, 816
EcoT14I CCWWGG 1 cut(s) 896
ErhI CCWWGG 1 cut(s) 896
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 5 cut(s) 85, 155, 443, 730, 838
FaqI GGGAC 3 cut(s) 76, 293, 722
FatI CATG 5 cut(s) 81, 151, 439, 726, 834
FauI CCCGC 1 cut(s) 318
FblI GTMKAC 2 cut(s) 831, 883
Fnu4HI GCNGC 4 cut(s) 161, 426, 560, 585
FokI GGATG 1 cut(s) 559
Fsp4HI GCNGC 4 cut(s) 161, 426, 560, 585
FspBI CTAG 3 cut(s) 495, 597, 771
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 3 cut(s) 254, 314, 918
GluI GCNGC 4 cut(s) 161, 426, 560, 585
GsaI CCCAGC 1 cut(s) 881
HaeIII GGCC 4 cut(s) 532, 606, 895, 926
HgaI GACGC 1 cut(s) 599
HhaI GCGC 3 cut(s) 255, 315, 919
Hin1II CATG 5 cut(s) 85, 155, 443, 730, 838
Hin6I GCGC 3 cut(s) 253, 313, 917
HinP1I GCGC 3 cut(s) 253, 313, 917
HinfI GANTC 6 cut(s) 71, 212, 219, 486, 650, 907
HphI GGTGA 5 cut(s) 10, 16, 206, 528, 566
Hpy166II GTNNAC 6 cut(s) 79, 116, 517, 832, 884, 935
Hpy188I TCNGA 6 cut(s) 70, 239, 357, 376, 491, 846
Hpy188III TCNNGA 4 cut(s) 108, 216, 397, 613
Hpy8I GTNNAC 6 cut(s) 79, 116, 517, 832, 884, 935
HpyAV CCTTC 3 cut(s) 476, 586, 664
HpyCH4III ACNGT 2 cut(s) 249, 951
HpyCH4V TGCA 6 cut(s) 290, 344, 425, 562, 730, 781
HpyF10VI GCNNNNNNNGC 5 cut(s) 319, 341, 590, 599, 923
HpyF3I CTNAG 5 cut(s) 236, 398, 614, 639, 761
Hsp92II CATG 5 cut(s) 85, 155, 443, 730, 838
HspAI GCGC 3 cut(s) 253, 313, 917
Kzo9I GATC 3 cut(s) 119, 260, 497
LmnI GCTCC 1 cut(s) 598
Lsp1109I GCAGC 3 cut(s) 437, 546, 596
LweI GCATC 2 cut(s) 348, 578
MaeI CTAG 3 cut(s) 495, 597, 771
MaeIII GTNAC 2 cut(s) 16, 194
MalI GATC 3 cut(s) 121, 262, 499
MboI GATC 3 cut(s) 119, 260, 497
MboII GAAGA 3 cut(s) 459, 836, 902
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 1 cut(s) 844
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 2 cut(s) 405, 718
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 679
Mox20I TGGCCA 1 cut(s) 606
MscI TGGCCA 1 cut(s) 606
MseI TTAA 2 cut(s) 267, 870
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 2 cut(s) 428, 587
MspR9I CCNGG 3 cut(s) 13, 415, 818
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 3 cut(s) 13, 415, 818
MvnI CGCG 1 cut(s) 313
MwoI GCNNNNNNNGC 5 cut(s) 319, 341, 590, 599, 923
NdeII GATC 3 cut(s) 119, 260, 497
NlaIII CATG 5 cut(s) 85, 155, 443, 730, 838
NlaIV GGNNCC 3 cut(s) 309, 756, 894
NmuCI GTSAC 2 cut(s) 16, 194
NsbI TGCGCA 1 cut(s) 254
NspI RCATGY 2 cut(s) 85, 730
PaeI GCATGC 1 cut(s) 730
PasI CCCWGGG 2 cut(s) 12, 414
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PctI GAATGC 1 cut(s) 162
PfeI GAWTC 5 cut(s) 212, 219, 486, 650, 907
PkrI GCNGC 4 cut(s) 162, 427, 561, 586
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 865
Psp5II RGGWCCY 1 cut(s) 865
Psp6I CCWGG 3 cut(s) 11, 413, 816
PspEI GGTNACC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 3 cut(s) 11, 413, 816
PspN4I GGNNCC 3 cut(s) 309, 756, 894
PspPI GGNCC 5 cut(s) 307, 530, 865, 893, 925
PspPPI RGGWCCY 1 cut(s) 865
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PstI CTGCAG 3 cut(s) 346, 427, 564
PstNI CAGNNNCTG 2 cut(s) 74, 613
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 2 cut(s) 428, 587
SaqAI TTAA 2 cut(s) 267, 870
SatI GCNGC 4 cut(s) 161, 426, 560, 585
Sau3AI GATC 3 cut(s) 119, 260, 497
Sau96I GGNCC 5 cut(s) 307, 530, 865, 893, 925
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 3 cut(s) 13, 415, 818
SduI GDGCHC 1 cut(s) 844
SfaNI GCATC 2 cut(s) 348, 578
SfcI CTRYAG 3 cut(s) 342, 423, 560
SinI GGWCC 2 cut(s) 307, 865
SphI GCATGC 1 cut(s) 730
Sse9I AATT 2 cut(s) 405, 718
SsiI CCGC 3 cut(s) 160, 311, 944
SspMI CTAG 3 cut(s) 495, 597, 771
StyD4I CCNGG 3 cut(s) 11, 413, 816
StyI CCWWGG 1 cut(s) 896
TaaI ACNGT 2 cut(s) 249, 951
TaqI TCGA 2 cut(s) 5, 226
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 405, 718
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 5 cut(s) 212, 219, 486, 650, 907
Tru1I TTAA 2 cut(s) 267, 870
Tru9I TTAA 2 cut(s) 267, 870
TscAI CASTG 1 cut(s) 351
TseFI GTSAC 2 cut(s) 16, 194
TseI GCWGC 3 cut(s) 425, 559, 584
Tsp45I GTSAC 2 cut(s) 16, 194
TspDTI ATGAA 1 cut(s) 194
TspRI CASTG 1 cut(s) 351
VpaK11BI GGWCC 2 cut(s) 307, 865
XapI RAATTY 1 cut(s) 405
XceI RCATGY 2 cut(s) 85, 730
XmiI GTMKAC 2 cut(s) 831, 883
XspI CTAG 3 cut(s) 495, 597, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.