Rh4DG423000

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001403
Physical Location & Seq
Reverse (-)
63024328 .. 63028598
4271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG423000.1

Sequence Viewer

Length: 894 bp
ATGTCAGGGTCTTCCAGGGGCCAAGTCACCATTACGCTTGGGCGTGGCCAGGTAGTAAAGAGGGCTGTTTCGGGTTCGGGTGCTGAGTCTCTACCCACGGCCGGGACGAAACGGTCGGTTAGAGATAGGTTAGGGAGTAATGCTGATAGCTCTGTGTCGAATGGAAGTGTAGGCAACAGCAACAAACGCCAGCGAGGACATAGTGGTATGTCAAGTTTGAACTCCAATGGTTGTGATGATCCATGCATTGGTAAAGACGACCTCCGGTTTAAACTCATGCAAAAAAATGCATGTAGACGGGCTCAGAGTGATGATGATGATCAGAGGCATGGAGATCTTCGTGAGAAGCTGTCAAAGGCAGTTCAGCGTCCTGTTCCTAGCGTTGAATCACGGTCACGGCAGCACCTGCCTGAACCACAAGAGAGTGGTTTTTTCGGTCGAGTTCCTTCTACGAGAAGTGCAGATGTTTTGCCTCGAGGTCCTGCTACAAGAAGTGCAGATGATTTGCCTCATATGGACTCGTTGAGAAATTCTTATTCTCCTTGGACACTGGACCATTTACGACGAAGATCCCCAGAAAGAGTTCAAGTCACTTATAGGGGTCTCTCCCCTCAGAGGAACGTAGAAGACTTGCAGAGAAGGCCATTAAACAGGACATATGATGATGGCAGATCTGTTCCATACATGAACAAAGATGTTCTAGACGCTACAAGGCCTGCGAGTAATGCACCTTTCATGTCTAATACTGCACTGCCTCCTGGACCTGCAAAGCATATGGCAGCCGCCCCGCCCCATATGAACCAAGTTCCTCCACTGAGTAGCACTGTACAGAAACCTCCATATGTGGTACATGTCATTTACTGTTTGCTCTTATGTTTTGTTGCCCCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

297

Amino Acids

32.56

Weight (kDa)

10.06

Isoelectric Point (pI)

71.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 414
AasI GACNNNNNNGTC 1 cut(s) 112
AbsI CCTCGAGG 1 cut(s) 474
Acc36I ACCTGC 2 cut(s) 414, 772
AccB7I CCANNNNNTGG 1 cut(s) 248
AccI GTMKAC 1 cut(s) 295
AciI CCGC 2 cut(s) 783, 788
AclWI GGATC 2 cut(s) 233, 564
AcoI YGGCCR 2 cut(s) 46, 99
AcsI RAATTY 1 cut(s) 529
AfaI GTAC 2 cut(s) 828, 849
AfiI CCNNNNNNNGG 4 cut(s) 101, 102, 248, 615
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 3 cut(s) 220, 386, 587
AjnI CCWGG 3 cut(s) 14, 48, 757
AluBI AGCT 2 cut(s) 150, 349
AluI AGCT 2 cut(s) 150, 349
Alw26I GTCTC 2 cut(s) 93, 608
AlwI GGATC 2 cut(s) 233, 564
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 5 cut(s) 19, 46, 99, 641, 713
ApeKI GCWGC 2 cut(s) 400, 779
ApoI RAATTY 1 cut(s) 529
Asp700I GAANNNNTTC 1 cut(s) 582
AspS9I GGNCC 4 cut(s) 19, 479, 553, 761
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 3 cut(s) 479, 553, 761
BalI TGGCCA 1 cut(s) 48
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 2 cut(s) 3, 633
BbvI GCAGC 2 cut(s) 412, 791
BccI CCATC 1 cut(s) 659
BceAI ACGGC 2 cut(s) 114, 413
BciT130I CCWGG 3 cut(s) 16, 50, 759
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 93, 608
BfaI CTAG 3 cut(s) 378, 701, 892
BfuAI ACCTGC 2 cut(s) 414, 772
BglII AGATCT 2 cut(s) 334, 671
BisI GCNGC 3 cut(s) 401, 780, 783
BlsI GCNGC 3 cut(s) 402, 781, 784
Bme1390I CCNGG 4 cut(s) 16, 50, 103, 759
Bme18I GGWCC 3 cut(s) 479, 553, 761
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 4 cut(s) 19, 479, 553, 761
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 4 cut(s) 16, 50, 103, 759
BpiI GAAGAC 2 cut(s) 3, 633
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 3 cut(s) 15, 96, 542
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 102, 248, 615
Bse1I ACTGG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 3 cut(s) 16, 50, 759
BseDI CCNNGG 3 cut(s) 15, 96, 542
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 4 cut(s) 101, 102, 248, 615
BseMII CTCAG 4 cut(s) 75, 317, 626, 806
BseNI ACTGG 1 cut(s) 555
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 412, 791
BsgI GTGCAG 3 cut(s) 480, 516, 732
Bsh1285I CGRYCG 3 cut(s) 102, 116, 439
BshFI GGCC 5 cut(s) 21, 48, 101, 643, 715
BsiEI CGRYCG 3 cut(s) 102, 116, 439
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 2 cut(s) 102, 265
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 4 cut(s) 101, 102, 248, 615
BsmAI GTCTC 2 cut(s) 93, 608
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 5 cut(s) 21, 48, 101, 643, 715
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 304
Bsp1407I TGTACA 1 cut(s) 826
Bsp143I GATC 5 cut(s) 238, 319, 334, 569, 671
BspACI CCGC 2 cut(s) 783, 788
BspANI GGCC 5 cut(s) 21, 48, 101, 643, 715
BspCNI CTCAG 4 cut(s) 76, 316, 625, 807
BspLI GGNNCC 1 cut(s) 20
BspMI ACCTGC 2 cut(s) 414, 772
BspPI GGATC 2 cut(s) 233, 564
BspTNI GGTCTC 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 826
BsrI ACTGG 1 cut(s) 555
BssECI CCNNGG 3 cut(s) 15, 96, 542
BssMI GATC 5 cut(s) 238, 319, 334, 569, 671
BssT1I CCWWGG 1 cut(s) 542
Bst2UI CCWGG 3 cut(s) 16, 50, 759
Bst4CI ACNGT 4 cut(s) 114, 393, 826, 863
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 826
BstC8I GCNNGC 2 cut(s) 191, 717
BstDEI CTNAG 4 cut(s) 84, 303, 612, 815
BstDSI CCRYGG 1 cut(s) 96
BstKTI GATC 5 cut(s) 241, 322, 337, 572, 674
BstMAI GTCTC 2 cut(s) 93, 608
BstMBI GATC 5 cut(s) 238, 319, 334, 569, 671
BstMCI CGRYCG 3 cut(s) 102, 116, 439
BstMWI GCNNNNNNNGC 4 cut(s) 186, 406, 640, 725
BstNI CCWGG 3 cut(s) 16, 50, 759
BstNSI RCATGY 2 cut(s) 294, 854
BstSCI CCNGG 4 cut(s) 14, 48, 101, 757
BstV1I GCAGC 2 cut(s) 412, 791
BstV2I GAAGAC 2 cut(s) 3, 633
BstX2I RGATCY 3 cut(s) 334, 569, 671
BstYI RGATCY 3 cut(s) 334, 569, 671
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 5 cut(s) 21, 48, 101, 643, 715
BtgI CCRYGG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 749
BtsIMutI CAGTG 4 cut(s) 548, 749, 812, 822
BveI ACCTGC 2 cut(s) 414, 772
Cac8I GCNNGC 2 cut(s) 191, 717
CaiI CAGNNNCTG 1 cut(s) 406
Cfr13I GGNCC 4 cut(s) 19, 479, 553, 761
CseI GACGC 2 cut(s) 356, 713
Csp6I GTAC 2 cut(s) 827, 848
CviAII CATG 7 cut(s) 243, 277, 291, 329, 685, 736, 851
CviQI GTAC 2 cut(s) 827, 848
DdeI CTNAG 4 cut(s) 84, 303, 612, 815
DpnI GATC 5 cut(s) 240, 321, 336, 571, 673
DpnII GATC 5 cut(s) 238, 319, 334, 569, 671
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 2 cut(s) 46, 99
EagI CGGCCG 1 cut(s) 99
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 542
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 3 cut(s) 479, 553, 761
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 479
EcoRII CCWGG 3 cut(s) 14, 48, 757
EcoT14I CCWWGG 1 cut(s) 542
EcoT22I ATGCAT 2 cut(s) 248, 292
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 7 cut(s) 246, 280, 294, 332, 688, 739, 854
FaqI GGGAC 1 cut(s) 118
FatI CATG 7 cut(s) 242, 276, 290, 328, 684, 735, 850
FauI CCCGC 1 cut(s) 795
FauNDI CATATG 5 cut(s) 513, 658, 774, 795, 841
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 401, 780, 783
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 401, 780, 783
FspBI CTAG 3 cut(s) 378, 701, 892
GluI GCNGC 3 cut(s) 401, 780, 783
HaeIII GGCC 5 cut(s) 21, 48, 101, 643, 715
HapII CCGG 2 cut(s) 102, 265
HgaI GACGC 2 cut(s) 356, 713
Hin1II CATG 7 cut(s) 246, 280, 294, 332, 688, 739, 854
HinfI GANTC 3 cut(s) 86, 386, 518
HpaII CCGG 2 cut(s) 102, 265
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 3 cut(s) 306, 324, 615
Hpy188III TCNNGA 2 cut(s) 341, 701
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 1 cut(s) 567
HpyAV CCTTC 2 cut(s) 456, 633
HpyCH4III ACNGT 4 cut(s) 114, 393, 826, 863
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 9 cut(s) 246, 280, 290, 461, 497, 634, 728, 749, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 406, 640, 725
HpyF3I CTNAG 4 cut(s) 84, 303, 612, 815
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 7 cut(s) 246, 280, 294, 332, 688, 739, 854
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 238, 319, 334, 569, 671
Lsp1109I GCAGC 2 cut(s) 412, 791
MaeI CTAG 3 cut(s) 378, 701, 892
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 3 cut(s) 25, 393, 589
MalI GATC 5 cut(s) 240, 321, 336, 571, 673
MboI GATC 5 cut(s) 238, 319, 334, 569, 671
MboII GAAGA 4 cut(s) 3, 329, 579, 638
MflI RGATCY 3 cut(s) 334, 569, 671
MhlI GDGCHC 1 cut(s) 304
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 1 cut(s) 529
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 95, 512
Mox20I TGGCCA 1 cut(s) 48
Mph1103I ATGCAT 2 cut(s) 248, 292
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 270, 647
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 102, 265
MspR9I CCNGG 4 cut(s) 16, 50, 103, 759
MssI GTTTAAAC 1 cut(s) 271
MvaI CCWGG 3 cut(s) 16, 50, 759
MwoI GCNNNNNNNGC 4 cut(s) 186, 406, 640, 725
NciI CCSGG 1 cut(s) 103
NdeI CATATG 5 cut(s) 513, 658, 774, 795, 841
NdeII GATC 5 cut(s) 238, 319, 334, 569, 671
NlaIII CATG 7 cut(s) 246, 280, 294, 332, 688, 739, 854
NlaIV GGNNCC 1 cut(s) 20
NmuCI GTSAC 3 cut(s) 25, 393, 589
NsiI ATGCAT 2 cut(s) 248, 292
NspI RCATGY 2 cut(s) 294, 854
PaeR7I CTCGAG 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 414
PceI AGGCCT 1 cut(s) 715
PciI ACATGT 1 cut(s) 850
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 3 cut(s) 402, 781, 784
PleI GAGTC 2 cut(s) 94, 512
PmeI GTTTAAAC 1 cut(s) 271
PpsI GAGTC 2 cut(s) 94, 512
PpuMI RGGWCCY 1 cut(s) 479
PscI ACATGT 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 3 cut(s) 14, 48, 757
PspGI CCWGG 3 cut(s) 14, 48, 757
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 4 cut(s) 19, 479, 553, 761
PspPPI RGGWCCY 1 cut(s) 479
PspXI VCTCGAGB 1 cut(s) 474
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 3 cut(s) 334, 569, 671
RsaI GTAC 2 cut(s) 828, 849
RsaNI GTAC 2 cut(s) 827, 848
SaqAI TTAA 2 cut(s) 270, 647
SatI GCNGC 3 cut(s) 401, 780, 783
Sau3AI GATC 5 cut(s) 238, 319, 334, 569, 671
Sau96I GGNCC 4 cut(s) 19, 479, 553, 761
SchI GAGTC 2 cut(s) 95, 512
ScrFI CCNGG 4 cut(s) 16, 50, 103, 759
SduI GDGCHC 1 cut(s) 304
Sfr274I CTCGAG 1 cut(s) 474
SinI GGWCC 3 cut(s) 479, 553, 761
SlaI CTCGAG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 1 cut(s) 529
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 2 cut(s) 783, 788
SspMI CTAG 3 cut(s) 378, 701, 892
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 4 cut(s) 14, 48, 101, 757
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 4 cut(s) 114, 393, 826, 863
TaiI ACGT 1 cut(s) 624
TaqI TCGA 3 cut(s) 158, 439, 475
TaqII GACCGA 1 cut(s) 425
TasI AATT 1 cut(s) 529
TatI WGTACW 1 cut(s) 826
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 270, 647
Tru9I TTAA 2 cut(s) 270, 647
TscAI CASTG 4 cut(s) 555, 756, 819, 829
TseFI GTSAC 3 cut(s) 25, 393, 589
TseI GCWGC 2 cut(s) 400, 779
Tsp45I GTSAC 3 cut(s) 25, 393, 589
TspDTI ATGAA 3 cut(s) 701, 724, 812
TspRI CASTG 4 cut(s) 555, 756, 819, 829
Van91I CCANNNNNTGG 1 cut(s) 248
VpaK11BI GGWCC 3 cut(s) 479, 553, 761
XapI RAATTY 1 cut(s) 529
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 2 cut(s) 294, 854
XhoI CTCGAG 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 3 cut(s) 378, 701, 892
Zsp2I ATGCAT 2 cut(s) 248, 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.