pycom16g07370

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001403
Physical Location & Seq
Forward (+)
4827370 .. 4830795
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07370.1

Sequence Viewer

Length: 1017 bp
ATGTCAGGGAATTCGAGGGGTCGAGTCACCATCACGCTTGGGCGCGGTGGCCAGGTAGTGAAGAGGTCTGTTTCGGGGTCTTCATTAACTGATTCCATACCCGTGGCTGGGACCAAGCGGTCTGTTAGAGATAGGTTAGGAAGTAATGTAGACGGTTCTTTGTCGCATGGAAGCTTACACAACAATAAACGCCAGCGAGGAGACAATGGTAGGTCAAGTTTGAATTTCAATGGTTACGATGATGCACACATTGGTAAAGGTGATCTTCGATTTAAACTCATGCAAAAAAATGCATTTAGACGAGCTCAGAGTGATGACGGTCAGAGGCATGGGGACCTTCGTGAGAAGCTTTCAAAGGCAGTTCAGCGCCCTGTCACTGTCCATGACTCACGTCAGCACCTTCCTGAAATAAAAGAGACTAGCTTTATACGTCGAATTCCTTCTTCAAGGGCAGATGATTTGCCTCATGTGGACTCGTTAAGAAACTCTTATTCCCCTTGGACTCTGGACCATTTAAGACGAAGATCCCCAGATAGAGTTGTAGTCACTTCTAGGGGTCTCTCCTCTCCGAGGAATCCCAAAGAATTGGAGAGAAGGCCACTAAGCAGGGCATTTGATGATGTCAGATTGGATCCATATATGAGGAAAGATGTTCCCGGTGCTACAAGGCCTGCAAGTAGTGCTCCTTTCATGTCCAGTTCTCCATTGCCTCATGTGTCTGCGAAACCTGTGGCCCCACCCCCACCCCACTTGAGTCAAATTCCTCCACTGAGCAGCATTGTACAGAAAAGTTCATATGCGGGTGATGAACAGCAGACTGTAGATGGCTTGCTACATACGCTGGGATTGGGAAAATATGCCATTATTTTTAAGGCTGAGGAAATTGATATGACCGCATTGAAGCAGATGGGAGAAAATGATCTCAAAGAGCTGGGAATACCTATGGGACCTAGGAAGAAGCTTCTTCTTGCTCTCCTGCCTCGTTCCAAGCGGCCTCCAGCGACAAATGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

339

Amino Acids

37.33

Weight (kDa)

10.57

Isoelectric Point (pI)

62.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 271 - 325 6.4e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 272 - 324 6.5e-08 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 45
AciI CCGC 5 cut(s) 45, 118, 800, 894, 991
AclWI GGATC 3 cut(s) 519, 626, 639
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 4 cut(s) 10, 223, 435, 759
AfaI GTAC 2 cut(s) 783, 1011
AfiI CCNNNNNNNGG 3 cut(s) 107, 108, 570
AgsI TTSAA 5 cut(s) 223, 229, 354, 447, 901
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 6 cut(s) 174, 305, 349, 423, 931, 961
AluI AGCT 6 cut(s) 174, 305, 349, 423, 931, 961
Alw21I GWGCWC 2 cut(s) 307, 685
Alw26I GTCTC 3 cut(s) 195, 410, 563
AlwI GGATC 3 cut(s) 519, 626, 639
AoxI GGCC 5 cut(s) 49, 596, 668, 732, 992
ApeKI GCWGC 1 cut(s) 774
ApoI RAATTY 4 cut(s) 10, 223, 435, 759
Asp700I GAANNNNTTC 1 cut(s) 439
AspA2I CCTAGG 1 cut(s) 950
AspLEI GCGC 2 cut(s) 45, 369
AspS9I GGNCC 5 cut(s) 111, 334, 508, 733, 947
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 3 cut(s) 19, 272, 815
AvaII GGWCC 4 cut(s) 111, 334, 508, 947
AvrII CCTAGG 1 cut(s) 950
BalI TGGCCA 1 cut(s) 51
BamHI GGATCC 1 cut(s) 631
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 307, 685
BbvCI CCTCAGC 1 cut(s) 876
BbvI GCAGC 1 cut(s) 786
BccI CCATC 3 cut(s) 38, 818, 901
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 3 cut(s) 195, 410, 563
BfaI CTAG 3 cut(s) 420, 552, 951
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 370
BisI GCNGC 2 cut(s) 775, 992
BlnI CCTAGG 1 cut(s) 950
BlsI GCNGC 2 cut(s) 776, 993
Bme1390I CCNGG 2 cut(s) 53, 657
Bme18I GGWCC 4 cut(s) 111, 334, 508, 947
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 5 cut(s) 111, 334, 508, 733, 947
BmiI GGNNCC 5 cut(s) 112, 335, 633, 735, 948
BmrFI CCNGG 2 cut(s) 53, 657
BmsI GCATC 1 cut(s) 232
BoxI GACNNNNGTC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 981
Bpu10I CCTNAGC 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 772
BpuMI CCSGG 1 cut(s) 657
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 4 cut(s) 102, 497, 569, 950
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 108, 570
Bse1I ACTGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 704
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 102, 497, 569, 950
BseLI CCNNNNNNNGG 3 cut(s) 107, 108, 570
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 3 cut(s) 320, 761, 867
BseNI ACTGG 1 cut(s) 696
BseRI GAGGAG 2 cut(s) 213, 553
BseXI GCAGC 1 cut(s) 786
BseYI CCCAGC 3 cut(s) 107, 841, 931
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 51, 598, 670, 734, 994
BsiHKAI GWGCWC 2 cut(s) 307, 685
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 3 cut(s) 124, 347, 960
BslI CCNNNNNNNGG 3 cut(s) 107, 108, 570
BsmAI GTCTC 3 cut(s) 195, 410, 563
BsmFI GGGAC 3 cut(s) 124, 347, 960
BsnI GGCC 5 cut(s) 51, 598, 670, 734, 994
Bso31I GGTCTC 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 307, 685
Bsp1407I TGTACA 2 cut(s) 781, 1009
Bsp143I GATC 4 cut(s) 262, 524, 631, 919
BspACI CCGC 5 cut(s) 45, 118, 800, 894, 991
BspANI GGCC 5 cut(s) 51, 598, 670, 734, 994
BspCNI CTCAG 3 cut(s) 319, 762, 868
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 5 cut(s) 112, 335, 633, 735, 948
BspPI GGATC 3 cut(s) 519, 626, 639
BspTNI GGTCTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 704
BsrGI TGTACA 2 cut(s) 781, 1009
BsrI ACTGG 1 cut(s) 696
BssECI CCNNGG 4 cut(s) 102, 497, 569, 950
BssMI GATC 4 cut(s) 262, 524, 631, 919
BssT1I CCWWGG 2 cut(s) 497, 950
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 4 cut(s) 155, 320, 379, 820
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 680
BstAUI TGTACA 2 cut(s) 781, 1009
BstC8I GCNNGC 3 cut(s) 194, 672, 830
BstDEI CTNAG 4 cut(s) 306, 602, 770, 876
BstDSI CCRYGG 1 cut(s) 102
BstENI CCTNNNNNAGG 1 cut(s) 568
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 2 cut(s) 45, 369
BstKTI GATC 4 cut(s) 265, 527, 634, 922
BstMAI GTCTC 3 cut(s) 195, 410, 563
BstMBI GATC 4 cut(s) 262, 524, 631, 919
BstMWI GCNNNNNNNGC 2 cut(s) 680, 838
BstNI CCWGG 1 cut(s) 53
BstPAI GACNNNNGTC 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 51, 655
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 786
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 524, 631
BstXI CCANNNNNNTGG 2 cut(s) 103, 586
BstYI RGATCY 2 cut(s) 524, 631
BsuRI GGCC 5 cut(s) 51, 598, 670, 734, 994
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 392
BtsIMutI CAGTG 2 cut(s) 375, 767
Cac8I GCNNGC 3 cut(s) 194, 672, 830
CfoI GCGC 2 cut(s) 45, 369
Cfr13I GGNCC 5 cut(s) 111, 334, 508, 733, 947
Csp6I GTAC 2 cut(s) 782, 1010
CspCI CAANNNNNGTGG 2 cut(s) 732, 767
CviAII CATG 7 cut(s) 167, 280, 329, 383, 467, 691, 713
CviQI GTAC 2 cut(s) 782, 1010
DdeI CTNAG 4 cut(s) 306, 602, 770, 876
DpnI GATC 4 cut(s) 264, 526, 633, 921
DpnII GATC 4 cut(s) 262, 524, 631, 919
DraI TTTAAA 1 cut(s) 274
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 497, 950
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 4 cut(s) 111, 334, 508, 947
Eco53kI GAGCTC 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 568
EcoO109I RGGNCCY 2 cut(s) 334, 947
EcoRI GAATTC 2 cut(s) 10, 435
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 497, 950
EcoT22I ATGCAT 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 497, 950
FaeI CATG 7 cut(s) 170, 283, 332, 386, 470, 694, 716
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 472, 504
FaqI GGGAC 3 cut(s) 124, 347, 960
FatI CATG 7 cut(s) 166, 279, 328, 382, 466, 690, 712
FauI CCCGC 1 cut(s) 793
FauNDI CATATG 1 cut(s) 796
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 775, 992
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 2 cut(s) 775, 992
FspBI CTAG 3 cut(s) 420, 552, 951
GlaI GCGC 2 cut(s) 44, 368
GluI GCNGC 2 cut(s) 775, 992
GsaI CCCAGC 3 cut(s) 111, 845, 935
GsuI CTGGAG 1 cut(s) 981
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 5 cut(s) 51, 598, 670, 734, 994
HapII CCGG 1 cut(s) 657
HhaI GCGC 2 cut(s) 45, 369
Hin1II CATG 7 cut(s) 170, 283, 332, 386, 470, 694, 716
Hin6I GCGC 2 cut(s) 43, 367
HinP1I GCGC 2 cut(s) 43, 367
HindIII AAGCTT 3 cut(s) 172, 347, 959
HinfI GANTC 7 cut(s) 24, 92, 386, 473, 502, 574, 754
HpaII CCGG 1 cut(s) 657
HphI GGTGA 3 cut(s) 19, 272, 815
Hpy166II GTNNAC 2 cut(s) 151, 472
Hpy188I TCNGA 4 cut(s) 309, 324, 570, 626
Hpy188III TCNNGA 3 cut(s) 341, 404, 506
Hpy8I GTNNAC 2 cut(s) 151, 472
Hpy99I CGWCG 1 cut(s) 435
HpyAV CCTTC 4 cut(s) 347, 410, 450, 588
HpyCH4III ACNGT 4 cut(s) 155, 320, 379, 820
HpyCH4IV ACGT 2 cut(s) 391, 430
HpyCH4V TGCA 4 cut(s) 245, 283, 293, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 680, 838
HpyF3I CTNAG 4 cut(s) 306, 602, 770, 876
HpySE526I ACGT 2 cut(s) 391, 430
Hsp92II CATG 7 cut(s) 170, 283, 332, 386, 470, 694, 716
HspAI GCGC 2 cut(s) 43, 367
Kzo9I GATC 4 cut(s) 262, 524, 631, 919
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 1 cut(s) 786
LweI GCATC 1 cut(s) 232
MaeI CTAG 3 cut(s) 420, 552, 951
MaeII ACGT 2 cut(s) 391, 430
MaeIII GTNAC 4 cut(s) 25, 233, 373, 544
MalI GATC 4 cut(s) 264, 526, 633, 921
MboI GATC 4 cut(s) 262, 524, 631, 919
MboII GAAGA 7 cut(s) 72, 73, 257, 435, 534, 956, 967
MflI RGATCY 2 cut(s) 524, 631
MhlI GDGCHC 2 cut(s) 307, 685
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 6 cut(s) 10, 223, 435, 584, 759, 882
MluNI TGGCCA 1 cut(s) 51
MlyI GAGTC 5 cut(s) 33, 380, 467, 496, 763
Mox20I TGGCCA 1 cut(s) 51
Mph1103I ATGCAT 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 439
MscI TGGCCA 1 cut(s) 51
MseI TTAA 5 cut(s) 86, 273, 479, 515, 870
MslI CAYNNNNRTG 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 51
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 2 cut(s) 53, 657
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 680, 838
NciI CCSGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 796
NdeII GATC 4 cut(s) 262, 524, 631, 919
NlaIII CATG 7 cut(s) 170, 283, 332, 386, 470, 694, 716
NlaIV GGNNCC 5 cut(s) 112, 335, 633, 735, 948
NmuCI GTSAC 3 cut(s) 25, 373, 544
NsiI ATGCAT 1 cut(s) 295
PceI AGGCCT 1 cut(s) 670
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 2 cut(s) 92, 574
PkrI GCNGC 2 cut(s) 776, 993
PleI GAGTC 5 cut(s) 32, 380, 467, 496, 762
PpsI GAGTC 5 cut(s) 32, 380, 467, 496, 762
PpuMI RGGWCCY 2 cut(s) 334, 947
PshAI GACNNNNGTC 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 2 cut(s) 334, 947
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 3 cut(s) 107, 841, 931
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 5 cut(s) 112, 335, 633, 735, 948
PspPI GGNCC 5 cut(s) 111, 334, 508, 733, 947
PspPPI RGGWCCY 2 cut(s) 334, 947
PsuI RGATCY 2 cut(s) 524, 631
RsaI GTAC 2 cut(s) 783, 1011
RsaNI GTAC 2 cut(s) 782, 1010
RseI CAYNNNNRTG 1 cut(s) 101
SacI GAGCTC 1 cut(s) 307
SaqAI TTAA 5 cut(s) 86, 273, 479, 515, 870
SatI GCNGC 2 cut(s) 775, 992
Sau3AI GATC 4 cut(s) 262, 524, 631, 919
Sau96I GGNCC 5 cut(s) 111, 334, 508, 733, 947
SchI GAGTC 5 cut(s) 33, 380, 467, 496, 763
ScrFI CCNGG 2 cut(s) 53, 657
SduI GDGCHC 2 cut(s) 307, 685
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 4 cut(s) 111, 334, 508, 947
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
Sse9I AATT 6 cut(s) 10, 223, 435, 584, 759, 882
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 5 cut(s) 45, 118, 800, 894, 991
SspMI CTAG 3 cut(s) 420, 552, 951
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 2 cut(s) 51, 655
StyI CCWWGG 2 cut(s) 497, 950
TaaI ACNGT 4 cut(s) 155, 320, 379, 820
TaiI ACGT 2 cut(s) 394, 433
TaqI TCGA 4 cut(s) 14, 22, 268, 433
TasI AATT 6 cut(s) 10, 223, 435, 584, 759, 882
TatI WGTACW 2 cut(s) 781, 1009
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 2 cut(s) 92, 574
Tru1I TTAA 5 cut(s) 86, 273, 479, 515, 870
Tru9I TTAA 5 cut(s) 86, 273, 479, 515, 870
TscAI CASTG 2 cut(s) 382, 774
TseFI GTSAC 3 cut(s) 25, 373, 544
TseI GCWGC 1 cut(s) 774
Tsp45I GTSAC 3 cut(s) 25, 373, 544
TspDTI ATGAA 4 cut(s) 72, 679, 783, 822
TspRI CASTG 2 cut(s) 382, 774
VpaK11BI GGWCC 4 cut(s) 111, 334, 508, 947
XagI CCTNNNNNAGG 1 cut(s) 568
XapI RAATTY 4 cut(s) 10, 223, 435, 759
XmaJI CCTAGG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 3 cut(s) 420, 552, 951
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.