Rh4BG427000

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001403
Physical Location & Seq
Reverse (-)
57090821 .. 57094951
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG427000.1

Sequence Viewer

Length: 960 bp
ATGTCAGGGTCTTCCAGGGGCCAAGTCACCATTACGCTTGGGCGTGGCCAGGTAGTAAAGAGGGCTGTTTCGGGTTCGGGTGCTGAGTCTCTACCCACGGCCGGGACGAAACGGTCGGTTAGAGATAGGTTAGGGAGTAATGCTGATAGCTCTGTGTCGAATGGAAGTGTAGGCAACAGCAACAAACGCCAGCGAGGACATAGTGGTATGTCAAGTTTGAACTCCAATGGTTGTGATGTTTTAACCTCTCTGCCTTTAAATCCACAAGATCCATGCATTGGTAAAGACGACCTCCGGTTTAAACTCATGCAAAAAAATGCATGTAGACGGGCTCAGAGTGATGATGATGATCAGAGGCATGGAGATCTTCGTGAGAAGCTGTCAAAGGCAGTTCAGCGTCCTGTTCCTAGCGTTGAATCACGGTCACGGCAGCGCCTGCCTGAACCACAAGAGAGTGGTTTTTTCGGTCGAGTTCCTTCTACGAGAAGTGCAGATGTTTTGCCTCGAGGTCCTGCTACAAGAAGTGCAGATGATTTGCCTCATATGGACTCGTTGAGAAATTCTTATTCTCCTTGGACACTGGACCATTTACGACGAAGATCCCCAGAAAGAGTTCAAGTCACTTATAGGGGTCTCTCCCCTCAGAGGAACGTAGAAGACTTGCAGAGAAGGCCATTAAACAGGACATATGATGATGGCAGATCTGCTCCATACATGAACAAAGATGTTCTAGACGCTACAAGGCCTGCGAGTACTGCACCTTTCATGGACCTAGGAAAAAGCTTCTGCTTGCTCTCTTGCCTCGTTCCAAGCGGCCACTGCCCACAAATATACGTTAGGAGTGGATACATTGCGTCTGTGGATGCAAAAATGTTGGCTTTTACTTTCGGATTAAAACTGAATTTATATTGTGATTATATAAATCTTCTGCTGCATGCCTGGTCTGGTTCTGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

319

Amino Acids

35.15

Weight (kDa)

9.77

Isoelectric Point (pI)

69.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 112
AbsI CCTCGAGG 1 cut(s) 504
AccB7I CCANNNNNTGG 1 cut(s) 278
AccI GTMKAC 1 cut(s) 325
AciI CCGC 1 cut(s) 813
AclWI GGATC 2 cut(s) 263, 594
AcoI YGGCCR 3 cut(s) 46, 99, 814
AcsI RAATTY 2 cut(s) 559, 901
AfaI GTAC 1 cut(s) 754
AfiI CCNNNNNNNGG 4 cut(s) 101, 102, 278, 645
AgsI TTSAA 3 cut(s) 220, 416, 617
AjnI CCWGG 3 cut(s) 14, 48, 938
AluBI AGCT 3 cut(s) 150, 379, 783
AluI AGCT 3 cut(s) 150, 379, 783
Alw26I GTCTC 2 cut(s) 93, 638
AlwI GGATC 2 cut(s) 263, 594
AlwNI CAGNNNCTG 1 cut(s) 436
Ama87I CYCGRG 1 cut(s) 504
AoxI GGCC 6 cut(s) 19, 46, 99, 671, 743, 814
ApeKI GCWGC 2 cut(s) 430, 931
ApoI RAATTY 2 cut(s) 559, 901
Asp700I GAANNNNTTC 1 cut(s) 612
AspA2I CCTAGG 1 cut(s) 772
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 4 cut(s) 19, 509, 583, 769
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 504
AvaII GGWCC 3 cut(s) 509, 583, 769
AvrII CCTAGG 1 cut(s) 772
BalI TGGCCA 1 cut(s) 48
BanII GRGCYC 1 cut(s) 334
BbsI GAAGAC 2 cut(s) 3, 663
BbvI GCAGC 2 cut(s) 442, 918
BccI CCATC 1 cut(s) 689
BceAI ACGGC 2 cut(s) 114, 443
BciT130I CCWGG 3 cut(s) 16, 50, 940
BciVI GTATCC 1 cut(s) 839
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 93, 638
BfaI CTAG 3 cut(s) 408, 731, 773
BfoI RGCGCY 1 cut(s) 436
BfuI GTATCC 1 cut(s) 839
BglII AGATCT 2 cut(s) 364, 701
BisI GCNGC 3 cut(s) 431, 814, 932
BlnI CCTAGG 1 cut(s) 772
BlsI GCNGC 3 cut(s) 432, 815, 933
BmcAI AGTACT 1 cut(s) 754
Bme1390I CCNGG 4 cut(s) 16, 50, 103, 940
Bme18I GGWCC 3 cut(s) 509, 583, 769
BmeT110I CYCGRG 1 cut(s) 504
BmgT120I GGNCC 4 cut(s) 19, 509, 583, 769
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 4 cut(s) 16, 50, 103, 940
BmsI GCATC 1 cut(s) 853
BpiI GAAGAC 2 cut(s) 3, 663
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 4 cut(s) 15, 96, 572, 772
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 102, 278, 645
Bse1I ACTGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 3 cut(s) 16, 50, 940
BseDI CCNNGG 4 cut(s) 15, 96, 572, 772
BseGI GGATG 1 cut(s) 868
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 4 cut(s) 101, 102, 278, 645
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 4 cut(s) 75, 347, 656, 942
BseNI ACTGG 1 cut(s) 585
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 442, 918
BsgI GTGCAG 3 cut(s) 510, 546, 741
Bsh1285I CGRYCG 3 cut(s) 102, 116, 469
BshFI GGCC 6 cut(s) 21, 48, 101, 673, 745, 816
BsiEI CGRYCG 3 cut(s) 102, 116, 469
BsiHKCI CYCGRG 1 cut(s) 504
BsiSI CCGG 2 cut(s) 102, 295
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 4 cut(s) 101, 102, 278, 645
BsmAI GTCTC 2 cut(s) 93, 638
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 6 cut(s) 21, 48, 101, 673, 745, 816
Bso31I GGTCTC 1 cut(s) 638
BsoBI CYCGRG 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 5 cut(s) 268, 349, 364, 599, 701
BspACI CCGC 1 cut(s) 813
BspANI GGCC 6 cut(s) 21, 48, 101, 673, 745, 816
BspCNI CTCAG 4 cut(s) 76, 346, 655, 943
BspLI GGNNCC 1 cut(s) 20
BspPI GGATC 2 cut(s) 263, 594
BspTNI GGTCTC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 849
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 4 cut(s) 15, 96, 572, 772
BssMI GATC 5 cut(s) 268, 349, 364, 599, 701
BssT1I CCWWGG 2 cut(s) 572, 772
Bst2UI CCWGG 3 cut(s) 16, 50, 940
Bst4CI ACNGT 2 cut(s) 114, 423
BstAPI GCANNNNNTGC 1 cut(s) 436
BstC8I GCNNGC 5 cut(s) 191, 437, 747, 791, 936
BstDEI CTNAG 4 cut(s) 84, 333, 642, 951
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 868
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 5 cut(s) 271, 352, 367, 602, 704
BstMAI GTCTC 2 cut(s) 93, 638
BstMBI GATC 5 cut(s) 268, 349, 364, 599, 701
BstMCI CGRYCG 3 cut(s) 102, 116, 469
BstMWI GCNNNNNNNGC 5 cut(s) 186, 436, 670, 755, 819
BstNI CCWGG 3 cut(s) 16, 50, 940
BstNSI RCATGY 2 cut(s) 324, 938
BstSCI CCNGG 4 cut(s) 14, 48, 101, 938
BstV1I GCAGC 2 cut(s) 442, 918
BstV2I GAAGAC 2 cut(s) 3, 663
BstX2I RGATCY 4 cut(s) 268, 364, 599, 701
BstYI RGATCY 4 cut(s) 268, 364, 599, 701
BstZI CGGCCG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 6 cut(s) 21, 48, 101, 673, 745, 816
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 868
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 2 cut(s) 578, 817
Cac8I GCNNGC 5 cut(s) 191, 437, 747, 791, 936
CaiI CAGNNNCTG 1 cut(s) 436
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 4 cut(s) 19, 509, 583, 769
CseI GACGC 3 cut(s) 386, 743, 843
Csp6I GTAC 1 cut(s) 753
CviAII CATG 7 cut(s) 273, 307, 321, 359, 715, 766, 935
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 4 cut(s) 84, 333, 642, 951
DpnI GATC 5 cut(s) 270, 351, 366, 601, 703
DpnII GATC 5 cut(s) 268, 349, 364, 599, 701
DraI TTTAAA 2 cut(s) 258, 301
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 3 cut(s) 46, 99, 814
EagI CGGCCG 1 cut(s) 99
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 572, 772
Eco147I AGGCCT 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 638
Eco47I GGWCC 3 cut(s) 509, 583, 769
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 14, 48, 938
EcoT14I CCWWGG 2 cut(s) 572, 772
EcoT22I ATGCAT 2 cut(s) 278, 322
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 572, 772
FaeI CATG 7 cut(s) 276, 310, 324, 362, 718, 769, 938
FaqI GGGAC 1 cut(s) 118
FatI CATG 7 cut(s) 272, 306, 320, 358, 714, 765, 934
FauNDI CATATG 2 cut(s) 543, 688
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 431, 814, 932
FokI GGATG 1 cut(s) 875
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 431, 814, 932
FspBI CTAG 3 cut(s) 408, 731, 773
GlaI GCGC 1 cut(s) 434
GluI GCNGC 3 cut(s) 431, 814, 932
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 6 cut(s) 21, 48, 101, 673, 745, 816
HapII CCGG 2 cut(s) 102, 295
HgaI GACGC 3 cut(s) 386, 743, 843
HhaI GCGC 1 cut(s) 435
Hin1II CATG 7 cut(s) 276, 310, 324, 362, 718, 769, 938
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 781
HinfI GANTC 3 cut(s) 86, 416, 548
HpaII CCGG 2 cut(s) 102, 295
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 5 cut(s) 336, 354, 645, 890, 952
Hpy188III TCNNGA 2 cut(s) 371, 731
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 1 cut(s) 597
HpyAV CCTTC 2 cut(s) 486, 663
HpyCH4III ACNGT 2 cut(s) 114, 423
HpyCH4IV ACGT 2 cut(s) 651, 834
HpyCH4V TGCA 9 cut(s) 276, 310, 320, 491, 527, 664, 758, 866, 934
HpyF10VI GCNNNNNNNGC 5 cut(s) 186, 436, 670, 755, 819
HpyF3I CTNAG 4 cut(s) 84, 333, 642, 951
HpySE526I ACGT 2 cut(s) 651, 834
Hsp92II CATG 7 cut(s) 276, 310, 324, 362, 718, 769, 938
HspAI GCGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 5 cut(s) 268, 349, 364, 599, 701
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 2 cut(s) 442, 918
LweI GCATC 1 cut(s) 853
MaeI CTAG 3 cut(s) 408, 731, 773
MaeII ACGT 2 cut(s) 651, 834
MaeIII GTNAC 3 cut(s) 25, 423, 619
MalI GATC 5 cut(s) 270, 351, 366, 601, 703
MboI GATC 5 cut(s) 268, 349, 364, 599, 701
MboII GAAGA 5 cut(s) 3, 359, 609, 668, 917
MflI RGATCY 4 cut(s) 268, 364, 599, 701
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 2 cut(s) 559, 901
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 95, 542
Mox20I TGGCCA 1 cut(s) 48
Mph1103I ATGCAT 2 cut(s) 278, 322
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 1 cut(s) 48
MseI TTAA 5 cut(s) 242, 257, 300, 677, 893
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 102, 295
MspR9I CCNGG 4 cut(s) 16, 50, 103, 940
MssI GTTTAAAC 1 cut(s) 301
MvaI CCWGG 3 cut(s) 16, 50, 940
MwoI GCNNNNNNNGC 5 cut(s) 186, 436, 670, 755, 819
NciI CCSGG 1 cut(s) 103
NdeI CATATG 2 cut(s) 543, 688
NdeII GATC 5 cut(s) 268, 349, 364, 599, 701
NlaIII CATG 7 cut(s) 276, 310, 324, 362, 718, 769, 938
NlaIV GGNNCC 1 cut(s) 20
NmuCI GTSAC 3 cut(s) 25, 423, 619
NsiI ATGCAT 2 cut(s) 278, 322
NspI RCATGY 2 cut(s) 324, 938
PaeI GCATGC 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 504
PceI AGGCCT 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 278
PkrI GCNGC 3 cut(s) 432, 815, 933
PleI GAGTC 2 cut(s) 94, 542
PmeI GTTTAAAC 1 cut(s) 301
PpsI GAGTC 2 cut(s) 94, 542
PpuMI RGGWCCY 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 3 cut(s) 14, 48, 938
PspGI CCWGG 3 cut(s) 14, 48, 938
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 4 cut(s) 19, 509, 583, 769
PspPPI RGGWCCY 1 cut(s) 509
PspXI VCTCGAGB 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 436
PsuI RGATCY 4 cut(s) 268, 364, 599, 701
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
SaqAI TTAA 5 cut(s) 242, 257, 300, 677, 893
SatI GCNGC 3 cut(s) 431, 814, 932
Sau3AI GATC 5 cut(s) 268, 349, 364, 599, 701
Sau96I GGNCC 4 cut(s) 19, 509, 583, 769
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 2 cut(s) 95, 542
ScrFI CCNGG 4 cut(s) 16, 50, 103, 940
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 1 cut(s) 853
Sfr274I CTCGAG 1 cut(s) 504
SinI GGWCC 3 cut(s) 509, 583, 769
SlaI CTCGAG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
SphI GCATGC 1 cut(s) 938
Sse9I AATT 2 cut(s) 559, 901
SseBI AGGCCT 1 cut(s) 745
SsiI CCGC 1 cut(s) 813
SspMI CTAG 3 cut(s) 408, 731, 773
StuI AGGCCT 1 cut(s) 745
StyD4I CCNGG 4 cut(s) 14, 48, 101, 938
StyI CCWWGG 2 cut(s) 572, 772
TaaI ACNGT 2 cut(s) 114, 423
TaiI ACGT 2 cut(s) 654, 837
TaqI TCGA 3 cut(s) 158, 469, 505
TaqII GACCGA 1 cut(s) 455
TasI AATT 2 cut(s) 559, 901
TatI WGTACW 1 cut(s) 752
TauI GCSGC 1 cut(s) 816
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 5 cut(s) 242, 257, 300, 677, 893
Tru9I TTAA 5 cut(s) 242, 257, 300, 677, 893
TscAI CASTG 2 cut(s) 585, 824
TseFI GTSAC 3 cut(s) 25, 423, 619
TseI GCWGC 2 cut(s) 430, 931
Tsp45I GTSAC 3 cut(s) 25, 423, 619
TspDTI ATGAA 2 cut(s) 731, 754
TspRI CASTG 2 cut(s) 585, 824
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 509, 583, 769
XapI RAATTY 2 cut(s) 559, 901
XbaI TCTAGA 1 cut(s) 730
XceI RCATGY 2 cut(s) 324, 938
XhoI CTCGAG 1 cut(s) 504
XmaJI CCTAGG 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 3 cut(s) 408, 731, 773
ZrmI AGTACT 1 cut(s) 754
Zsp2I ATGCAT 2 cut(s) 278, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.