Rw2G051330

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001403
Physical Location & Seq
Forward (+)
79068330 .. 79070702
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051330.1

Sequence Viewer

Length: 960 bp
ATGTCGAAGCCCAGGGTCACCATCACCCTCGGTCGCTCTGGTCAGGTTGTGGAGAGGGGAGGGACATCAGACTCTGTAAATATGCGTGGCGAAAGAGGTATGGTTTCGGGAGGTAGACGATCAATGAGAGACAGGCTTGGGAGTAATGCTTATGGAAAGCGGCAACGAGGAGATGGAAATGGAATAAAATGGAGAGGGGGTGACAATACATTACATGATTCACGAATCAGTCGAAATGACCTCAGATTGAAACTGTTGCGCAAAAGATCATTTAAGCAAAATGGGGGAGTTGTTGCAGAACGCAAGCAGATGGTCCCGCGTCCAAAGCTTTCAAAACCTGCTCATTCTGCAGTGAGGTATCAGATGCCACCGCACAAGTCAGAAACAAATGAGAGTAGCTTCCTGAGGCAAATTCCTCCCAGGGAAACTGCAGCTGATTTGTATCATGTAAATCCACAGAGGAGCTTCAACTCTTCTCAAAGTAGAGAAGGATTCCGAGCTAGATCCCCCGATAACTTTGTGAACACTTCTTATGGGCCATCACCTCAAAGAAACTTTGGTGAGCTGCAGTCAGCATCATCCTTCAGGGCAGCTGACGCTCCTAGAGCTGGCCAGTTCCTGAGAAATGGTATGGTTGGTTCGTCTCAGCCAACAGATTCCTTCCTTTTCAGAACAAAATCTAACCCTCAAACTGCCAAGCCAGTTGGACAGCATGTGCCAACAAATTACAGCATGCAAAAAGTCCCTTATATGGGGGAGGAACCTCTAAGTGTTGCTAGTTTATTGCATAGACTTGGTTTGGGCAAATATGCTATTATTTTCCAGGCTGAAGAAGTAGACATGGGTGCTCTGAAGCAGATGGGGGATAAGGACCTTAAAGAGCTGGGTATACCAATGGGGCCAAGGAAAAAGATTCTTCTTGCGCTTTTGGCCCGCGGGAAACGACCGCCACCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

319

Amino Acids

35.38

Weight (kDa)

11.02

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 256 - 309 1.2e-12 SAM domain (Sterile alpha motif)
SAM_2 PF07647 257 - 308 3.6e-07 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 3 cut(s) 115, 837, 889
AccII CGCG 2 cut(s) 319, 936
AciI CCGC 6 cut(s) 160, 317, 371, 934, 936, 947
AclWI GGATC 1 cut(s) 498
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 1 cut(s) 411
AcuI CTGAAG 3 cut(s) 568, 849, 872
AfiI CCNNNNNNNGG 3 cut(s) 608, 751, 752
AgsI TTSAA 3 cut(s) 250, 333, 469
AjnI CCWGG 3 cut(s) 11, 419, 822
AluBI AGCT 9 cut(s) 328, 399, 434, 465, 500, 565, 593, 608, 883
AluI AGCT 9 cut(s) 328, 399, 434, 465, 500, 565, 593, 608, 883
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 2 cut(s) 123, 648
AlwI GGATC 1 cut(s) 498
AlwNI CAGNNNCTG 2 cut(s) 74, 619
AoxI GGCC 4 cut(s) 536, 610, 899, 930
ApeKI GCWGC 3 cut(s) 431, 565, 590
ApoI RAATTY 1 cut(s) 411
AspLEI GCGC 2 cut(s) 261, 925
AspS9I GGNCC 5 cut(s) 313, 536, 871, 899, 931
AsuHPI GGTGA 5 cut(s) 10, 16, 212, 534, 572
AvaII GGWCC 2 cut(s) 313, 871
AxyI CCTNAGG 1 cut(s) 404
BalI TGGCCA 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 3 cut(s) 443, 552, 602
BccI CCATC 5 cut(s) 29, 167, 304, 547, 853
BciT130I CCWGG 3 cut(s) 13, 421, 824
BcoDI GTCTC 2 cut(s) 123, 648
BfaI CTAG 3 cut(s) 501, 603, 777
BfmI CTRYAG 3 cut(s) 348, 429, 566
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 4 cut(s) 161, 432, 566, 591
BlsI GCNGC 4 cut(s) 162, 433, 567, 592
Bme1390I CCNGG 3 cut(s) 13, 421, 824
Bme18I GGWCC 2 cut(s) 313, 871
BmgT120I GGNCC 5 cut(s) 313, 536, 871, 899, 931
BmiI GGNNCC 3 cut(s) 315, 762, 900
BmrFI CCNGG 3 cut(s) 13, 421, 824
BmsI GCATC 2 cut(s) 354, 584
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 7 cut(s) 11, 12, 28, 419, 420, 902, 934
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 751, 752
Bse1I ACTGG 2 cut(s) 613, 701
Bse21I CCTNAGG 1 cut(s) 404
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 3 cut(s) 13, 421, 824
BseDI CCNNGG 7 cut(s) 11, 12, 28, 419, 420, 902, 934
BseGI GGATG 1 cut(s) 578
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 608, 751, 752
BseMII CTCAG 4 cut(s) 256, 395, 611, 659
BseNI ACTGG 2 cut(s) 613, 701
BseRI GAGGAG 2 cut(s) 183, 475
BseXI GCAGC 3 cut(s) 443, 552, 602
BseYI CCCAGC 1 cut(s) 883
Bsh1236I CGCG 2 cut(s) 319, 936
Bsh1285I CGRYCG 2 cut(s) 34, 947
BshFI GGCC 4 cut(s) 538, 612, 901, 932
BsiEI CGRYCG 2 cut(s) 34, 947
BsiHKAI GWGCWC 1 cut(s) 850
BslFI GGGAC 3 cut(s) 76, 299, 728
BslI CCNNNNNNNGG 3 cut(s) 608, 751, 752
BsmAI GTCTC 2 cut(s) 123, 648
BsmBI CGTCTC 1 cut(s) 648
BsmFI GGGAC 3 cut(s) 76, 299, 728
BsnI GGCC 4 cut(s) 538, 612, 901, 932
Bsp1286I GDGCHC 1 cut(s) 850
Bsp143I GATC 3 cut(s) 119, 266, 503
BspACI CCGC 6 cut(s) 160, 317, 371, 934, 936, 947
BspANI GGCC 4 cut(s) 538, 612, 901, 932
BspCNI CTCAG 4 cut(s) 255, 396, 612, 658
BspFNI CGCG 2 cut(s) 319, 936
BspLI GGNNCC 3 cut(s) 315, 762, 900
BspMAI CTGCAG 3 cut(s) 352, 433, 570
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 498
BsrI ACTGG 2 cut(s) 613, 701
BssECI CCNNGG 7 cut(s) 11, 12, 28, 419, 420, 902, 934
BssMI GATC 3 cut(s) 119, 266, 503
BssNAI GTATAC 1 cut(s) 890
BssT1I CCWWGG 1 cut(s) 902
Bst1107I GTATAC 1 cut(s) 890
Bst2UI CCWGG 3 cut(s) 13, 421, 824
Bst4CI ACNGT 2 cut(s) 255, 957
Bst6I CTCTTC 1 cut(s) 478
BstC8I GCNNGC 4 cut(s) 305, 610, 734, 934
BstDEI CTNAG 5 cut(s) 242, 404, 620, 645, 767
BstDSI CCRYGG 1 cut(s) 934
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 1 cut(s) 578
BstFNI CGCG 2 cut(s) 319, 936
BstHHI GCGC 2 cut(s) 261, 925
BstKTI GATC 3 cut(s) 122, 269, 506
BstMAI GTCTC 2 cut(s) 123, 648
BstMBI GATC 3 cut(s) 119, 266, 503
BstMCI CGRYCG 2 cut(s) 34, 947
BstMWI GCNNNNNNNGC 5 cut(s) 325, 347, 596, 605, 929
BstNI CCWGG 3 cut(s) 13, 421, 824
BstNSI RCATGY 2 cut(s) 716, 736
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 3 cut(s) 11, 419, 822
BstSFI CTRYAG 3 cut(s) 348, 429, 566
BstUI CGCG 2 cut(s) 319, 936
BstV1I GCAGC 3 cut(s) 443, 552, 602
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BstZ17I GTATAC 1 cut(s) 890
Bsu36I CCTNAGG 1 cut(s) 404
BsuRI GGCC 4 cut(s) 538, 612, 901, 932
BtgI CCRYGG 1 cut(s) 934
BtsCI GGATG 1 cut(s) 578
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 305, 610, 734, 934
CaiI CAGNNNCTG 2 cut(s) 74, 619
CfoI GCGC 2 cut(s) 261, 925
Cfr13I GGNCC 5 cut(s) 313, 536, 871, 899, 931
Cfr42I CCGCGG 1 cut(s) 937
CseI GACGC 2 cut(s) 308, 605
CviAII CATG 5 cut(s) 215, 446, 713, 733, 841
DdeI CTNAG 5 cut(s) 242, 404, 620, 645, 767
DpnI GATC 3 cut(s) 121, 268, 505
DpnII GATC 3 cut(s) 119, 266, 503
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 902
Eco47I GGWCC 2 cut(s) 313, 871
Eco57I CTGAAG 3 cut(s) 568, 849, 872
Eco81I CCTNAGG 1 cut(s) 404
Eco91I GGTNACC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 871
EcoO65I GGTNACC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 11, 419, 822
EcoT14I CCWWGG 1 cut(s) 902
ErhI CCWWGG 1 cut(s) 902
Esp3I CGTCTC 1 cut(s) 648
FaeI CATG 5 cut(s) 218, 449, 716, 736, 844
FaqI GGGAC 3 cut(s) 76, 299, 728
FatI CATG 5 cut(s) 214, 445, 712, 732, 840
FauI CCCGC 3 cut(s) 324, 929, 941
FblI GTMKAC 3 cut(s) 115, 837, 889
Fnu4HI GCNGC 4 cut(s) 161, 432, 566, 591
FokI GGATG 1 cut(s) 565
Fsp4HI GCNGC 4 cut(s) 161, 432, 566, 591
FspBI CTAG 3 cut(s) 501, 603, 777
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 2 cut(s) 260, 924
GluI GCNGC 4 cut(s) 161, 432, 566, 591
GsaI CCCAGC 1 cut(s) 887
HaeIII GGCC 4 cut(s) 538, 612, 901, 932
HgaI GACGC 2 cut(s) 308, 605
HhaI GCGC 2 cut(s) 261, 925
Hin1II CATG 5 cut(s) 218, 449, 716, 736, 844
Hin6I GCGC 2 cut(s) 259, 923
HinP1I GCGC 2 cut(s) 259, 923
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 6 cut(s) 71, 218, 225, 492, 656, 913
HphI GGTGA 5 cut(s) 10, 16, 212, 534, 572
Hpy166II GTNNAC 4 cut(s) 116, 523, 838, 890
Hpy188I TCNGA 7 cut(s) 70, 245, 363, 382, 497, 671, 852
Hpy188III TCNNGA 4 cut(s) 108, 222, 403, 619
Hpy8I GTNNAC 4 cut(s) 116, 523, 838, 890
HpyAV CCTTC 3 cut(s) 482, 592, 670
HpyCH4III ACNGT 2 cut(s) 255, 957
HpyCH4V TGCA 6 cut(s) 296, 350, 431, 568, 736, 787
HpyF10VI GCNNNNNNNGC 5 cut(s) 325, 347, 596, 605, 929
HpyF3I CTNAG 5 cut(s) 242, 404, 620, 645, 767
Hsp92II CATG 5 cut(s) 218, 449, 716, 736, 844
HspAI GCGC 2 cut(s) 259, 923
KspI CCGCGG 1 cut(s) 937
Kzo9I GATC 3 cut(s) 119, 266, 503
LmnI GCTCC 2 cut(s) 462, 604
Lsp1109I GCAGC 3 cut(s) 443, 552, 602
LweI GCATC 2 cut(s) 354, 584
MaeI CTAG 3 cut(s) 501, 603, 777
MaeIII GTNAC 2 cut(s) 16, 200
MalI GATC 3 cut(s) 121, 268, 505
MboI GATC 3 cut(s) 119, 266, 503
MboII GAAGA 3 cut(s) 465, 842, 908
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 1 cut(s) 850
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 2 cut(s) 411, 724
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 685
Mox20I TGGCCA 1 cut(s) 612
MscI TGGCCA 1 cut(s) 612
MseI TTAA 2 cut(s) 273, 876
Msp20I TGGCCA 1 cut(s) 612
MspA1I CMGCKG 3 cut(s) 434, 593, 936
MspR9I CCNGG 3 cut(s) 13, 421, 824
MvaI CCWGG 3 cut(s) 13, 421, 824
MvnI CGCG 2 cut(s) 319, 936
MwoI GCNNNNNNNGC 5 cut(s) 325, 347, 596, 605, 929
NdeII GATC 3 cut(s) 119, 266, 503
NlaIII CATG 5 cut(s) 218, 449, 716, 736, 844
NlaIV GGNNCC 3 cut(s) 315, 762, 900
NmuCI GTSAC 2 cut(s) 16, 200
NsbI TGCGCA 1 cut(s) 260
NspI RCATGY 2 cut(s) 716, 736
PaeI GCATGC 1 cut(s) 736
PasI CCCWGGG 2 cut(s) 12, 420
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PfeI GAWTC 5 cut(s) 218, 225, 492, 656, 913
PkrI GCNGC 4 cut(s) 162, 433, 567, 592
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 3 cut(s) 11, 419, 822
PspEI GGTNACC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 3 cut(s) 11, 419, 822
PspN4I GGNNCC 3 cut(s) 315, 762, 900
PspPI GGNCC 5 cut(s) 313, 536, 871, 899, 931
PspPPI RGGWCCY 1 cut(s) 871
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 3 cut(s) 352, 433, 570
PstNI CAGNNNCTG 2 cut(s) 74, 619
PsuI RGATCY 1 cut(s) 503
PvuII CAGCTG 2 cut(s) 434, 593
SacII CCGCGG 1 cut(s) 937
SaqAI TTAA 2 cut(s) 273, 876
SatI GCNGC 4 cut(s) 161, 432, 566, 591
Sau3AI GATC 3 cut(s) 119, 266, 503
Sau96I GGNCC 5 cut(s) 313, 536, 871, 899, 931
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 3 cut(s) 13, 421, 824
SduI GDGCHC 1 cut(s) 850
SfaNI GCATC 2 cut(s) 354, 584
SfcI CTRYAG 3 cut(s) 348, 429, 566
Sfr303I CCGCGG 1 cut(s) 937
SgrBI CCGCGG 1 cut(s) 937
SinI GGWCC 2 cut(s) 313, 871
SphI GCATGC 1 cut(s) 736
Sse9I AATT 2 cut(s) 411, 724
SsiI CCGC 6 cut(s) 160, 317, 371, 934, 936, 947
SspMI CTAG 3 cut(s) 501, 603, 777
StyD4I CCNGG 3 cut(s) 11, 419, 822
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 2 cut(s) 255, 957
TaqI TCGA 2 cut(s) 5, 232
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 411, 724
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 5 cut(s) 218, 225, 492, 656, 913
Tru1I TTAA 2 cut(s) 273, 876
Tru9I TTAA 2 cut(s) 273, 876
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 2 cut(s) 16, 200
TseI GCWGC 3 cut(s) 431, 565, 590
Tsp45I GTSAC 2 cut(s) 16, 200
TspRI CASTG 1 cut(s) 357
VpaK11BI GGWCC 2 cut(s) 313, 871
XapI RAATTY 1 cut(s) 411
XceI RCATGY 2 cut(s) 716, 736
XmiI GTMKAC 3 cut(s) 115, 837, 889
XspI CTAG 3 cut(s) 501, 603, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.