Rorug04G0355100

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001403
Physical Location & Seq
Reverse (-)
52728225 .. 52731258
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0355100.1

Sequence Viewer

Length: 1059 bp
ATGTCACGTTACGACTTCAGTGCTTCTGGAGTGAAACTGAAACCGAGAATGCAAAACGACGTCGCCTACTCCACCGCCTCAGCTCTCATCGGAACCTCCGCAATGTCATCATCGCAGCCTCAACCGTACGGCGGTGTTTACAGCTCCGTCTACTCCTCCTCGATCTTCGGTCCAAAGCCTTCTTCAGTCGCACCTTACGGCGTCCAATCAAACTCCGACACGTCGCAGGCCTACCGCAACAAACTCTACCAGTCTCTGCTGGTCCAAAACCGGCAGGAGATGGTCAACCGCCACAGCCTCTGCCTGAAGCGCCTCCACGAGGCGGCGGAGGAAGCCGACGCTCTCCGCCGTGAGAACAGCCACCTCCGCAACGTCAACGTCGAGCTGAACAAGCAACTCAGTCTCCTCATCCACGCCTCCATCCAGAAAGAGATCGAAGCTTCTTCAAGCTCTGTGCAGAGGTCGCCGTTTGGGATCGTCAACGGAATTCGCGGCCTGTCAATTGACAGCAAGTCGTCGGCTCAAGAGGAAGAGGAAGAGGAATTGAGCGATCAGAGTCCGACGAGTGAAGAGGCCGAGAATGTTGATGTGGAGAGGATTTCGCTGCCGAAGAGCATATCGGTGAGGTCCAACGGCTACGTGAAGCTTCCTCCTGTGACTGCTGCTACCAACGCCGCCGCTCGGACTCGGACCGCGGCCAGATCTCGAATCGCCAAGGAAATCAGTGCTTCACAAAAGGTGTACGTTCAAGGAGGGAAGAAAGAGGAGGTGCCACTTGAACTGGAGGTGTTCAACCAGGGAATGTTCAAGACTGAACTCTGCAACAAATGGCAGGAGTCTGGTGACTGCCCCTACAAAGACCACTGCCAATTTGCTCACGGCGTGGAGGAGCTGCGCCCGGTGATTCGCCACCCGCGCTACAAGACCGAGGTCTGCAGAATGGTGCTAGCCGGTGATGTCTGTCCATATGGCCATCGCTGCCATTTTCGCCACGCGCTCACTGAGGAGGAGAAGGAGCTTGCTGGTGGCAACAATACGCCCAGCAGGCCAATCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

352

Amino Acids

39.05

Weight (kDa)

8.09

Isoelectric Point (pI)

59.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 269 - 294 9.3e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 769
AccBSI CCGCTC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 150
AccII CGCG 4 cut(s) 492, 695, 916, 995
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 2 cut(s) 696, 970
AcsI RAATTY 1 cut(s) 486
AcuI CTGAAG 2 cut(s) 168, 326
AcyI GRCGYC 2 cut(s) 60, 201
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 2 cut(s) 128, 743
AfiI CCNNNNNNNGG 4 cut(s) 131, 319, 322, 681
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 5 cut(s) 447, 749, 779, 793, 808
AhdI GACNNNNNGTC 1 cut(s) 511
AjiI CACGTC 1 cut(s) 222
AjnI CCWGG 1 cut(s) 795
AjuI GAANNNNNNNTTGG 2 cut(s) 166, 198
AluBI AGCT 8 cut(s) 83, 144, 385, 440, 450, 646, 892, 1018
AluI AGCT 8 cut(s) 83, 144, 385, 440, 450, 646, 892, 1018
Alw26I GTCTC 2 cut(s) 258, 407
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 2 cut(s) 256, 300
AoxI GGCC 6 cut(s) 228, 493, 573, 696, 970, 1046
ApeKI GCWGC 5 cut(s) 115, 604, 662, 892, 978
ApoI RAATTY 1 cut(s) 486
AspLEI GCGC 4 cut(s) 312, 897, 918, 997
AspS9I GGNCC 4 cut(s) 170, 262, 627, 690
AsuC2I CCSGG 1 cut(s) 899
AsuHPI GGTGA 4 cut(s) 634, 854, 913, 965
AsuNHI GCTAGC 1 cut(s) 946
AvaII GGWCC 4 cut(s) 170, 262, 627, 690
BalI TGGCCA 1 cut(s) 972
BanI GGYRCC 1 cut(s) 769
BauI CACGAG 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 79
BbvI GCAGC 5 cut(s) 127, 591, 649, 879, 965
BccI CCATC 3 cut(s) 274, 428, 981
BceAI ACGGC 6 cut(s) 145, 214, 333, 451, 649, 895
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 797
BcnI CCSGG 1 cut(s) 899
BcoDI GTCTC 2 cut(s) 258, 407
BfaI CTAG 1 cut(s) 947
BfmI CTRYAG 1 cut(s) 934
BfoI RGCGCY 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 1045
BglII AGATCT 1 cut(s) 701
Bme1390I CCNGG 2 cut(s) 797, 899
Bme18I GGWCC 4 cut(s) 170, 262, 627, 690
BmeRI GACNNNNNGTC 1 cut(s) 511
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 4 cut(s) 170, 262, 627, 690
BmiI GGNNCC 2 cut(s) 94, 771
BmrFI CCNGG 2 cut(s) 797, 899
BmtI GCTAGC 1 cut(s) 950
BoxI GACNNNNGTC 1 cut(s) 929
BpmI CTGGAG 2 cut(s) 48, 803
Bpu10I CCTNAGC 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 507
BpuMI CCSGG 1 cut(s) 899
BsaAI YACGTR 1 cut(s) 640
BsaHI GRCGYC 2 cut(s) 60, 201
BsaJI CCNNGG 4 cut(s) 693, 714, 796, 927
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 319, 322, 681
Bse118I RCCGGY 2 cut(s) 270, 950
Bse1I ACTGG 2 cut(s) 250, 786
Bse3DI GCAATG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 797
BseDI CCNNGG 4 cut(s) 693, 714, 796, 927
BseGI GGATG 2 cut(s) 408, 420
BseLI CCNNNNNNNGG 4 cut(s) 131, 319, 322, 681
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 3 cut(s) 93, 412, 993
BseNI ACTGG 2 cut(s) 250, 786
BseRI GAGGAG 7 cut(s) 145, 148, 395, 779, 902, 1019, 1022
BseXI GCAGC 5 cut(s) 127, 591, 649, 879, 965
BseYI CCCAGC 1 cut(s) 1040
BsgI GTGCAG 1 cut(s) 476
Bsh1236I CGCG 4 cut(s) 492, 695, 916, 995
BshFI GGCC 6 cut(s) 230, 495, 575, 698, 972, 1048
BshNI GGYRCC 1 cut(s) 769
BsiSI CCGG 3 cut(s) 271, 899, 951
BsiWI CGTACG 1 cut(s) 126
BslI CCNNNNNNNGG 4 cut(s) 131, 319, 322, 681
BsmAI GTCTC 2 cut(s) 258, 407
BsmI GAATGC 1 cut(s) 54
BsnI GGCC 6 cut(s) 230, 495, 575, 698, 972, 1048
Bsp143I GATC 5 cut(s) 162, 432, 474, 550, 701
BspANI GGCC 6 cut(s) 230, 495, 575, 698, 972, 1048
BspCNI CTCAG 3 cut(s) 92, 411, 994
BspFNI CGCG 4 cut(s) 492, 695, 916, 995
BspLI GGNNCC 2 cut(s) 94, 771
BspMAI CTGCAG 1 cut(s) 938
BspOI GCTAGC 1 cut(s) 950
BspPI GGATC 1 cut(s) 482
BspQI GCTCTTC 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 769
BsrBI CCGCTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 270, 950
BsrI ACTGG 2 cut(s) 250, 786
BssAI RCCGGY 2 cut(s) 270, 950
BssECI CCNNGG 4 cut(s) 693, 714, 796, 927
BssMI GATC 5 cut(s) 162, 432, 474, 550, 701
BssNI GRCGYC 2 cut(s) 60, 201
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 714
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 1 cut(s) 126
Bst6I CTCTTC 4 cut(s) 525, 531, 564, 605
BstACI GRCGYC 2 cut(s) 60, 201
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 4 cut(s) 228, 948, 1020, 1046
BstDEI CTNAG 3 cut(s) 79, 398, 1002
BstDSI CCRYGG 1 cut(s) 693
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 408, 420
BstFNI CGCG 4 cut(s) 492, 695, 916, 995
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 4 cut(s) 312, 897, 918, 997
BstKTI GATC 5 cut(s) 165, 435, 477, 553, 704
BstMAI GTCTC 2 cut(s) 258, 407
BstMBI GATC 5 cut(s) 162, 432, 474, 550, 701
BstNI CCWGG 1 cut(s) 797
BstPAI GACNNNNGTC 1 cut(s) 929
BstSCI CCNGG 2 cut(s) 795, 897
BstSFI CTRYAG 1 cut(s) 934
BstUI CGCG 4 cut(s) 492, 695, 916, 995
BstV1I GCAGC 5 cut(s) 127, 591, 649, 879, 965
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
BsuRI GGCC 6 cut(s) 230, 495, 575, 698, 972, 1048
BtgI CCRYGG 1 cut(s) 693
BtgZI GCGATG 2 cut(s) 96, 959
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 2 cut(s) 408, 420
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 4 cut(s) 25, 730, 862, 999
Cac8I GCNNGC 4 cut(s) 228, 948, 1020, 1046
CaiI CAGNNNCTG 2 cut(s) 256, 300
CfoI GCGC 4 cut(s) 312, 897, 918, 997
Cfr10I RCCGGY 2 cut(s) 270, 950
Cfr13I GGNCC 4 cut(s) 170, 262, 627, 690
Cfr42I CCGCGG 1 cut(s) 696
CpoI CGGWCCG 1 cut(s) 690
CseI GACGC 2 cut(s) 190, 347
Csp6I GTAC 2 cut(s) 127, 742
CspI CGGWCCG 1 cut(s) 690
CviQI GTAC 2 cut(s) 127, 742
DdeI CTNAG 3 cut(s) 79, 398, 1002
DpnI GATC 5 cut(s) 164, 434, 476, 552, 703
DpnII GATC 5 cut(s) 162, 432, 474, 550, 701
DraIII CACNNNGTG 1 cut(s) 883
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 696, 970
Eam1104I CTCTTC 4 cut(s) 525, 531, 564, 605
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 4 cut(s) 525, 531, 564, 605
EciI GGCGGA 2 cut(s) 335, 341
Eco130I CCWWGG 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 230
Eco47I GGWCC 4 cut(s) 170, 262, 627, 690
Eco57I CTGAAG 2 cut(s) 168, 326
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
FaiI YATR 3 cut(s) 617, 967, 969
FauI CCCGC 1 cut(s) 921
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 1 cut(s) 150
FokI GGATG 2 cut(s) 395, 407
FspBI CTAG 1 cut(s) 947
GlaI GCGC 4 cut(s) 311, 896, 917, 996
GsaI CCCAGC 1 cut(s) 1044
GsuI CTGGAG 2 cut(s) 48, 803
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 6 cut(s) 230, 495, 575, 698, 972, 1048
HapII CCGG 3 cut(s) 271, 899, 951
HgaI GACGC 2 cut(s) 190, 347
HhaI GCGC 4 cut(s) 312, 897, 918, 997
Hin1I GRCGYC 2 cut(s) 60, 201
Hin6I GCGC 4 cut(s) 310, 895, 916, 995
HinP1I GCGC 4 cut(s) 310, 895, 916, 995
HincII GTYRAC 3 cut(s) 286, 376, 481
HindII GTYRAC 3 cut(s) 286, 376, 481
HindIII AAGCTT 2 cut(s) 438, 644
HinfI GANTC 5 cut(s) 556, 685, 708, 836, 904
HpaII CCGG 3 cut(s) 271, 899, 951
HphI GGTGA 4 cut(s) 634, 854, 913, 965
Hpy166II GTNNAC 6 cut(s) 139, 151, 286, 376, 481, 742
Hpy188I TCNGA 6 cut(s) 92, 217, 555, 561, 684, 690
Hpy188III TCNNGA 5 cut(s) 27, 424, 524, 705, 808
Hpy8I GTNNAC 6 cut(s) 139, 151, 286, 376, 481, 742
Hpy99I CGWCG 7 cut(s) 62, 65, 226, 341, 383, 520, 565
HpyAV CCTTC 2 cut(s) 189, 1006
HpyCH4III ACNGT 1 cut(s) 126
HpyCH4IV ACGT 7 cut(s) 7, 60, 221, 372, 378, 639, 744
HpyCH4V TGCA 4 cut(s) 52, 457, 822, 936
HpyF3I CTNAG 3 cut(s) 79, 398, 1002
HpySE526I ACGT 7 cut(s) 7, 60, 221, 372, 378, 639, 744
Hsp92I GRCGYC 2 cut(s) 60, 201
HspAI GCGC 4 cut(s) 310, 895, 916, 995
KspI CCGCGG 1 cut(s) 696
Kzo9I GATC 5 cut(s) 162, 432, 474, 550, 701
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 3 cut(s) 149, 889, 1015
Lsp1109I GCAGC 5 cut(s) 127, 591, 649, 879, 965
MaeI CTAG 1 cut(s) 947
MaeII ACGT 7 cut(s) 7, 60, 221, 372, 378, 639, 744
MaeIII GTNAC 4 cut(s) 3, 8, 655, 842
MalI GATC 5 cut(s) 164, 434, 476, 552, 703
MbiI CCGCTC 1 cut(s) 680
MboI GATC 5 cut(s) 162, 432, 474, 550, 701
MboII GAAGA 8 cut(s) 157, 174, 435, 542, 548, 581, 622, 769
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 1 cut(s) 701
MlsI TGGCCA 1 cut(s) 972
MluCI AATT 4 cut(s) 486, 501, 542, 869
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 3 cut(s) 565, 679, 845
MmeI TCCRAC 3 cut(s) 240, 584, 654
Mox20I TGGCCA 1 cut(s) 972
MscI TGGCCA 1 cut(s) 972
MslI CAYNNNNRTG 1 cut(s) 620
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 3 cut(s) 271, 899, 951
MspR9I CCNGG 2 cut(s) 797, 899
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 54
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 4 cut(s) 492, 695, 916, 995
NciI CCSGG 1 cut(s) 899
NdeI CATATG 1 cut(s) 967
NdeII GATC 5 cut(s) 162, 432, 474, 550, 701
NheI GCTAGC 1 cut(s) 946
NlaIV GGNNCC 2 cut(s) 94, 771
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 3 cut(s) 3, 655, 842
PceI AGGCCT 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 708, 904
Pfl23II CGTACG 1 cut(s) 126
PleI GAGTC 3 cut(s) 564, 679, 844
PpsI GAGTC 3 cut(s) 564, 679, 844
Ppu21I YACGTR 1 cut(s) 640
PshAI GACNNNNGTC 1 cut(s) 929
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 1040
PspGI CCWGG 1 cut(s) 795
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 94, 771
PspPI GGNCC 4 cut(s) 170, 262, 627, 690
PstI CTGCAG 1 cut(s) 938
PstNI CAGNNNCTG 2 cut(s) 256, 300
PsuI RGATCY 1 cut(s) 701
RsaI GTAC 2 cut(s) 128, 743
RsaNI GTAC 2 cut(s) 127, 742
RseI CAYNNNNRTG 1 cut(s) 620
Rsr2I CGGWCCG 1 cut(s) 690
RsrII CGGWCCG 1 cut(s) 690
SacII CCGCGG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 605
Sau3AI GATC 5 cut(s) 162, 432, 474, 550, 701
Sau96I GGNCC 4 cut(s) 170, 262, 627, 690
SchI GAGTC 3 cut(s) 565, 679, 845
ScrFI CCNGG 2 cut(s) 797, 899
SfcI CTRYAG 1 cut(s) 934
Sfr303I CCGCGG 1 cut(s) 696
SgrBI CCGCGG 1 cut(s) 696
SinI GGWCC 4 cut(s) 170, 262, 627, 690
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 522
SmoI CTYRAG 1 cut(s) 522
Sse9I AATT 4 cut(s) 486, 501, 542, 869
SseBI AGGCCT 1 cut(s) 230
SspMI CTAG 1 cut(s) 947
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 795, 897
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 1 cut(s) 126
TaiI ACGT 7 cut(s) 10, 63, 224, 375, 381, 642, 747
TaqI TCGA 4 cut(s) 161, 381, 435, 706
TaqII GACCGA 2 cut(s) 158, 941
TasI AATT 4 cut(s) 486, 501, 542, 869
TauI GCSGC 5 cut(s) 326, 495, 677, 680, 698
TfiI GAWTC 2 cut(s) 708, 904
TscAI CASTG 4 cut(s) 25, 730, 869, 1006
TseFI GTSAC 3 cut(s) 3, 655, 842
TseI GCWGC 5 cut(s) 115, 604, 662, 892, 978
Tsp45I GTSAC 3 cut(s) 3, 655, 842
TspGWI ACGGA 2 cut(s) 136, 498
TspRI CASTG 4 cut(s) 25, 730, 869, 1006
VpaK11BI GGWCC 4 cut(s) 170, 262, 627, 690
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 1 cut(s) 486
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 1 cut(s) 947
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.